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1.
Int J Syst Evol Microbiol ; 68(10): 3301-3306, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30152751

RESUMO

A novel dark pink pigmented bacterium, designated strain CPCC 100847T (deposited with strain code 0113-15), was isolated from the urban air of Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CPCC 100847T was related to members of the genus Roseomonas and had the highest 16S rRNA gene sequence similarity to Roseomonas aestuarii JC17T (97.5 %). A low level of DNA-DNA relatedness (18.7 %) with its closest type strain R. aestuarii JC17T (KCTC 22692T) proved that strain CPCC 100847T belonged to a unique genomic species. CPCC 100847T had many common characteristics of the genus Roseomonas, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Roseomonas species. Cells were Gram-negative, cocci- to oval-shaped, non-motile, non-endospore-forming and strictly aerobic. The respiratory ubiquinone was Q-10. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified phospholipid. The major fatty acids (>5 %) were C18 : 1ω7c, anteiso-C15 : 0, C16 : 0, iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Roseomonas. The name proposed for this species is Roseomonasglobiformis sp. nov., with CPCC 100847T (=KCTC 52094T) as the type strain. The DNA G+C composition is 65.2 mol%.


Assuntos
Microbiologia do Ar , Methylobacteriaceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , Pequim , DNA Bacteriano/genética , Ácidos Graxos/química , Methylobacteriaceae/genética , Methylobacteriaceae/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
2.
J Nat Prod ; 80(10): 2595-2601, 2017 10 27.
Artigo em Inglês | MEDLINE | ID: mdl-29016131

RESUMO

Thirty-three metabolites including five phenalenone derivatives (1-5), seven cytochalasins (6-12), thirteen butenolides (13-25), and eight phenyl derivatives (26-33) were isolated from Aspergillus sp. CPCC 400735 cultured on rice. The structures of all compounds were elucidated by NMR, MS, and CD experiments, of which 1-5 (asperphenalenones A-E), 6 (aspochalasin R), and 13 (aspulvinone R) were identified as new compounds. Specifically, asperphenalenones A-E (1-5) represent an unusual structure composed of a linear diterpene derivative linked to a phenalenone derivative via a C-C bond. Compounds 1, 4, 10, and 26 exhibited anti-HIV activity with IC50 values of 4.5, 2.4, 9.2, and 6.6 µM, respectively (lamivudine 0.1 µM; efavirenz, 0.4 × 10-3 µM).


Assuntos
4-Butirolactona/análogos & derivados , Fármacos Anti-HIV/isolamento & purificação , Fármacos Anti-HIV/farmacologia , Aspergillus/química , Citocalasinas/isolamento & purificação , Citocalasinas/farmacologia , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Fenalenos/isolamento & purificação , Fenalenos/farmacologia , 4-Butirolactona/química , 4-Butirolactona/isolamento & purificação , 4-Butirolactona/farmacologia , Fármacos Anti-HIV/química , China , Citocalasinas/química , Diterpenos/química , Endófitos/química , Concentração Inibidora 50 , Kadsura/microbiologia , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Fenalenos/química
3.
J Asian Nat Prod Res ; 19(6): 541-549, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28395517

RESUMO

Five monoterpenoids were isolated from the endophytic fungus Periconia sp. F-31, including three new carene-type monoterpenoids, 2-carene-5,8-diol (1), 2-carene-8,10-diol (2), 2-carene-8-acetamide (3), one new menthene-type monoterpenoid 8-hydroxy-1,7-expoxy-2-menthene (4), and one known monoterpenoid anethofuran (5). The structures of all compounds were elucidated based on a comprehensive spectroscopic data analysis, electronic circular dichroism (ECD), and calculated ECD.


Assuntos
Antineoplásicos/isolamento & purificação , Ascomicetos/química , Monoterpenos/isolamento & purificação , Antineoplásicos/química , Antineoplásicos/farmacologia , Dicroísmo Circular , Cristalografia por Raios X , Ensaios de Seleção de Medicamentos Antitumorais , Estrutura Molecular , Monoterpenos/química , Monoterpenos/farmacologia , Ressonância Magnética Nuclear Biomolecular
4.
Zhongguo Zhong Yao Za Zhi ; 40(12): 2349-51, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26591523

RESUMO

To investigate the secondary metabolites of endophytic fungi Pericinia sp. F-31. Column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds. Two compounds were isolated from the fermentation broth of Periconia sp. Their structures were identified as 5-(1-hydroxyhexyl) -6-methyl-2H-pyran-2-one (1) and 2-(3-hydroxy-4-methylphenyl) -propanoic acid (2). Compound 1 was a new lactone compound, compound 2 was new natural product, and the NMR data of compound 2 was reported for the first time.


Assuntos
Annona/microbiologia , Ascomicetos/metabolismo , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Endófitos/metabolismo , Lactonas/química , Lactonas/isolamento & purificação , Ascomicetos/química , Ascomicetos/genética , Ascomicetos/isolamento & purificação , Medicamentos de Ervas Chinesas/metabolismo , Endófitos/química , Endófitos/genética , Endófitos/isolamento & purificação , Lactonas/metabolismo , Espectrometria de Massas , Estrutura Molecular
5.
J Asian Nat Prod Res ; 17(6): 683-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26027676

RESUMO

A new 2-arylbenzofuran compound, 5-dehydroxy-moracin U (1), along with 10 known compounds (2-11), were isolated from cell cultures of Morus alba. Their structures were elucidated on the basis of extensive spectroscopic analyses. The anti-inflammatory activity assay of 1-8 showed that 2 and 8 exhibited significant inhibitory effect on LPS-induced NO production with the values of 76.4% and 98.7% at 10(- 5) M, respectively.


Assuntos
Benzofuranos/isolamento & purificação , Morus/química , Anti-Inflamatórios , Benzofuranos/química , Benzofuranos/farmacologia , Lipopolissacarídeos/farmacologia , Estrutura Molecular , Óxido Nítrico/biossíntese , Casca de Planta/química , Extratos Vegetais/química
6.
J Asian Nat Prod Res ; 17(6): 656-61, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26074011

RESUMO

Biotransformations of icariin (1) by cell suspension cultures of Glycyrrhiza uralensis and Morus alba yielded two new metabolites, icaruralins A and B (2 and 3), and one known metabolite, baohuoside I (4). Their structures were determined on the basis of extensive spectroscopic analysis. This is the first report that the cell suspension cultures of G. uralensis and M. alba possess deglycosylation functionality.


Assuntos
Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Glycyrrhiza uralensis/química , Morus/química , Biotransformação , Flavonoides/química , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Raízes de Plantas/química
7.
Chem Pharm Bull (Tokyo) ; 61(5): 576-80, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23649200

RESUMO

A new spiroketallactone, epi-danshenspiroketallactone A (1) and a new C18-norditerpenoid, normiltioane (2) along with 21 known compounds, were isolated from cell cultures of Salvia miltiorrhiza. Their structures were elucidated on the basis of extensive spectroscopic analyses. In the in vitro assays, the compounds 9-11, 21-23 exhibited the significant antitumor activity with the IC(50) ranges of 1.0-8.3 µM.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Técnicas de Cultura de Células , Diterpenos/farmacologia , Salvia miltiorrhiza/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Diterpenos/química , Diterpenos/isolamento & purificação , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Salvia miltiorrhiza/citologia , Relação Estrutura-Atividade
8.
J Asian Nat Prod Res ; 14(9): 913-7, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22924543

RESUMO

One new abietane-type norditerpenoid, named militibetin A (1), was isolated from the dry roots of Salvia miltiorrhiza, along with two known diterpenoids, yunnannin A (2) and ferruginol (3). Their structures were established by means of extensive spectroscopic analyses. In vitro, compounds 1-3 were found to show cytotoxicities against selected cancer cells, including P-388, HONE-1, and HT-29, and gave ED(50) values in the range of 2.9-5.4 µg ml(- 1).


Assuntos
/isolamento & purificação , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Salvia miltiorrhiza/química , /química , Animais , Antineoplásicos Fitogênicos/química , Ensaios de Seleção de Medicamentos Antitumorais , Células HT29 , Humanos , Leucemia P388 , Camundongos , Estrutura Molecular , Raízes de Plantas/química
9.
J Nat Med ; 66(2): 362-6, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21898133

RESUMO

A new coumestan (solalyratin A, 1) and a novel cyclic eight-membered α,ß-unsaturated ketone (solalyratin B, 3), together with three known compounds, puerariafuran (2), coumestrol (4) and 9-hydroxy-2',2'-dimethylpyrano[5',6':2,3]-coumestan (5), were isolated from the whole plant of Solanum lyratum. Their structures were elucidated on the basis of spectroscopic analyses. In vitro, compounds 1-5 showed anti-inflammatory activities, with IC(50) values in the range 6.3-9.1 µM.


Assuntos
Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Cumarínicos/química , Cumarínicos/farmacologia , Cetonas/química , Cetonas/farmacologia , Animais , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Ratos , Solanum
10.
Zhongguo Zhong Yao Za Zhi ; 37(24): 3738-42, 2012 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-23627170

RESUMO

The column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds from the EtOAc extract of medium and MeOH extract of cell cultures of Morus alba. Eight compounds were isolated. Based on physico-chemical properties and spectroscopic data, their structures were identified as isobavachalcone (1), genistein (2), norartocarpetin (3), albanin A (4), guangsangon E (5), mulberrofuran F (6), chalcomoracin (7), kuwanon J (8). Compounds 3-6 were isolated from the cell cultures of M. alba for the first time.


Assuntos
Técnicas de Cultura de Células/métodos , Morus/química , Folhas de Planta/química , Plantas Medicinais/química , Benzofuranos/isolamento & purificação , Chalconas/isolamento & purificação , Cromatografia em Gel/métodos , Cromatografia Líquida de Alta Pressão , Dextranos , Genisteína/isolamento & purificação , Morus/citologia , Folhas de Planta/citologia , Plantas Medicinais/citologia , Sílica Gel , Terpenos/isolamento & purificação
11.
Chem Pharm Bull (Tokyo) ; 59(12): 1541-4, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22130377

RESUMO

Two new terpenoids, (+)-(3S,6S,7R,8S)-periconone A (1) and (-)-(1R,4R,6S,7S)-2-caren-4,8-olide (2), have been isolated from an endophytic fungus Periconia sp., which was collected from the plant Annona muricata. Their structures were elucidated on the basis of extensive spectroscopic analyses. In the in vitro assays, the two compounds showed low cytotoxic activities against six human tumor cell lines (HCT-8, Bel-7402, BGC-823, A549, A2780 and MCF-7) with IC(50)>10(-5) M.


Assuntos
Annona/microbiologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Ascomicetos/química , Terpenos/química , Terpenos/farmacologia , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Diterpenos/química , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Modelos Moleculares , Neoplasias/tratamento farmacológico , Análise Espectral , Terpenos/isolamento & purificação
12.
Nat Prod Res ; 25(11): 1019-24, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21337255

RESUMO

Two new norditerpenoid alkaloids, named scutebarbatines M-N (1 and 2), were isolated from the whole plant of Scutellaria barbata D. Don. Their structures were established on the basis of detailed spectral analyses. In vitro, two new compounds showed significant cytotoxic activities against three human cancer lines (HONE-1 nasopharyngeal, KB oral epidermoid carcinoma and HT29 colorectal carcinoma cells), and gave IC50 values in the range of 3.5-6.3 µM.


Assuntos
Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Scutellaria/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Células HT29 , Humanos , Concentração Inibidora 50 , Espectroscopia de Ressonância Magnética , Estrutura Molecular
13.
Chem Pharm Bull (Tokyo) ; 58(6): 840-2, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20522996

RESUMO

Three new 4-hydroxyisoflavans, named lyratin A (1), lyratin B (2) and lyratin C (3), along with a known compound, 4,7,2'trihydroxy-4'-methoxyisoflavan (4), were isolated from the whole plant of Solanum lyratum. Their structures were established by means of detailed physical data analyses. In vitro, four compounds showed anti-inflammatory activities with inhibitory ratios of release of beta-glucuronidase from polymorphonuclear leukocytes of rats in the range of 30.3-38.6% at 10 microM.


Assuntos
Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Isoflavonas/química , Isoflavonas/farmacologia , Neutrófilos/efeitos dos fármacos , Solanum/química , Animais , Anti-Inflamatórios/isolamento & purificação , Neutrófilos/imunologia , Ratos
14.
Fitoterapia ; 81(7): 737-41, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20079810

RESUMO

Two new neo-clerodane diterpenoids have been isolated from the whole plant of Scutellaria barbata D. Don, and their structures were established by detailed spectral analyses as scutehenanine H (1) and 6-(2,3-epoxy-2-isopropyl-n-propoxyl)barbatin C (2). In vitro, the isolated two new compounds showed significant cytotoxic activities against three human cancer lines, and gave IC(50) values in the range OF 2.0-4.2 µΜ.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Diterpenos Clerodânicos/isolamento & purificação , Neoplasias/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Scutellaria/química , Antineoplásicos Fitogênicos/farmacologia , Antineoplásicos Fitogênicos/uso terapêutico , Linhagem Celular Tumoral , Diterpenos Clerodânicos/farmacologia , Diterpenos Clerodânicos/uso terapêutico , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/farmacologia
15.
J Nat Prod ; 72(10): 1793-7, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19785430

RESUMO

Six new neo-clerodane diterpenoid alkaloids, named scutehenanines A-D (1, 4, 5, 6), 6-O-acetylscutehenanine A (2), and 6-O-(2-carbonyl-3-methylbutanoyl)scutehenanine A (3), were isolated from the whole plant of Scutellaria barbata. Their structures were established on the basis of detailed physical data analyses. In vitro, the six new isolated compounds showed cytotoxic activities against three human cancer lines (HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells) and gave IC50 values in the range 2.8-6.4 microM.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Diterpenos Clerodânicos/isolamento & purificação , Diterpenos Clerodânicos/farmacologia , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Plantas Medicinais/química , Scutellaria/química , Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos Fitogênicos/química , Diterpenos Clerodânicos/química , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Humanos , Concentração Inibidora 50 , Estrutura Molecular
16.
J Asian Nat Prod Res ; 11(5): 451-6, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19504388

RESUMO

Two new neo-clerodane diterpenoid alkaloids, scutebarbatine O (1) and 6-O-nicotinoylscutebarbatine G (2), were isolated from the whole plant of Scutellaria barbata. Their structures were elucidated by spectroscopic methods including extensive 1D and 2D NMR analyses. In vitro, compounds 1 and 2 showed cytotoxic activities against three human tumor cell lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and with IC(50) values in the range of 2.1-5.7 microM.


Assuntos
Alcaloides/isolamento & purificação , Alcaloides/farmacologia , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Diterpenos Clerodânicos/isolamento & purificação , Diterpenos Clerodânicos/farmacologia , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Plantas Medicinais/química , Scutellaria/química , Alcaloides/química , Antineoplásicos Fitogênicos/química , Diterpenos Clerodânicos/química , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Células HT29 , Humanos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular
17.
Chem Pharm Bull (Tokyo) ; 57(4): 408-10, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19336938

RESUMO

Two new sesquiterpenoids, lyratol C (1) and lyratol D (2), together with two known sesquiterpenoids, dehydrovomifoliol (3) and blumenol A (4), were isolated from the whole plant of Solanum lyratum. Their structures were established by spectroscopic analyses. In vitro, the four compounds showed significant cytotoxic activities against three human cancer cell lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and gave IC(50) values in the range 3.7-8.1 microM.


Assuntos
Antineoplásicos/isolamento & purificação , Sesquiterpenos/isolamento & purificação , Solanum/química , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Sesquiterpenos/farmacologia
18.
Planta Med ; 75(4): 375-7, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19148863

RESUMO

Two new alkaloids, named suspensine A (1) and (-)-7'-O-methylegenine (2), along with two known alkaloids, (-)-egenine (3) and (-)-bicuculline (4), were isolated from the ethanolic extract of the fruits of Forsythia suspensa. Their structures were established by means of chemical methods and spectroscopic analyses. In vitro, four alkaloids showed anti-inflammatory activities, with inhibition rates of release of beta-glucuronidase from polymorphonuclear leukocytes of rats being in the range 34.8%-39.6% at 10 microM.


Assuntos
Alcaloides/química , Alcaloides/farmacologia , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacologia , Forsythia/química , Frutas/química , Estrutura Molecular
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