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1.
Int J Nanomedicine ; 17: 3269-3286, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35924260

RESUMO

Background: Naringin is a naturally occurring flavanone that promotes osteogenesis. Owing to the high lipophilicity, poor in vivo bioavailability, and extensive metabolic alteration upon administration, the clinical efficacy of naringin is understudied. Additionally, information on the molecular mechanism by which it promotes osteogenesis is limited. Methods: In this study, we prepared TAT & RGD peptide-modified naringin-loaded nanoparticles (TAT-RGD-NAR-NPs), evaluated their potency on the osteogenic differentiation of human dental pulp stem cells (hDPSCs), and studied its mechanism of action through metabolomic analysis. Results: The particle size and zeta potential of TAT-RGD-NAR-NPs were 160.70±2.05 mm and -20.77±0.47mV, respectively. The result of cell uptake assay showed that TAT-RGD-NAR-NPs could effectively enter hDPSCs. TAT-RGD-NAR-NPs had a more significant effect on cell proliferation and osteogenic differentiation promotion. Furthermore, in metabolomic analysis, naringin particles showed a strong influence on the glycerophospholipid metabolism pathway of hDPSCs. Specifically, it upregulated the expression of PLA2G3 and PLA2G1B (two isozymes of phospholipase A2, PLA2), increased the biosynthesis of lysophosphatidic acid (LPA). Conclusion: These results suggested that TAT-RGD-NPs might be used for transporting naringin to hDPSCs for modulating stem cell osteogenic differentiation. The metabolomic analysis was used for the first time to elucidate the mechanism by which naringin promotes hDPSCs osteogenesis by upregulating PLA2G3 and PLA2G1B.


Assuntos
Flavanonas , Nanopartículas , Diferenciação Celular/fisiologia , Proliferação de Células , Células Cultivadas , Polpa Dentária , Flavanonas/farmacologia , Fosfolipases A2 do Grupo IB/metabolismo , Fosfolipases A2 do Grupo III/metabolismo , Humanos , Lipossomos , Oligopeptídeos/metabolismo , Osteogênese , Células-Tronco
2.
Comput Intell Neurosci ; 2022: 7091476, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35755748

RESUMO

With the continuous development of computer science and technology, the level of medical image processing and analysis technology has been significantly improved. In order to further optimize the medical imaging technology and provide assistance for medical diagnosis and treatment, this study will explore the head CT image segmentation technology and three-dimensional reconstruction technology based on human anatomy, using two morphological operation methods of image expansion and image corrosion, as well as the triangulation method based on surface contour, Optimize CT image segmentation technology and three-dimensional reconstruction technology. The results show that the CT image segmentation technology based on human anatomy can obtain the more essential morphology and features of the target image, and significantly improve the image quality. The size of the threshold can have a certain impact on the 3D reconstruction effect and reconstruction time to a certain extent. The larger the threshold, the shorter the reconstruction time, but the worse the 3D reconstruction effect. This shows that the target image after fitting has a good reconstruction effect, but the threshold level should be kept at a low level. The head CT image segmentation technology and three-dimensional reconstruction technology based on human anatomy have good application effects and can be popularized and applied in clinical diagnosis and treatment.


Assuntos
Imageamento Tridimensional , Tomografia Computadorizada por Raios X , Humanos , Processamento de Imagem Assistida por Computador/métodos , Tecnologia , Tomografia Computadorizada por Raios X/métodos
3.
Front Bioeng Biotechnol ; 10: 915181, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35757798

RESUMO

Background: Bone tissue defect, one of the common orthopaedicdiseases, is traumatizing and affects patient's lifestyle. Although autologous and xenograft bone transplantations are performed in bone tissue engineering, clinical development of bone transplantation is limited because ofvarious factors, such as varying degrees of immune rejection, lack of bone sources, and secondary damage to bone harvesting. Methods: We synthesised a heparinised gelatine-hydroxyapatite-tricalcium phosphate (HG-HA-TCP) scaffold loaded with sustained-release vascular endothelial growth factor (VEGF) analysed their structure, mechanical properties, and biocompatibility. Additionally, the effects of HG-HA-TCP (VEGF) scaffolds on osteogenic differentiation and vascularisation of stem cells from human exfoliated deciduous teeth (SHED) in vitro and bone regeneration in vivo were investigated. Results: HG-HA-TCP scaffold possessed good pore structure, mechanical properties, and biocompatibility. HG-HA-TCP scaffold loaded with VEGF could effectively promote SHED proliferation, migration, and adhesion. Moreover, HG-HA-TCP (VEGF) scaffold increased the expression of osteogenesis- and angiogenesis-related genes and promoted osteogenic differentiation and vascularisation in cells. In vivo results demonstrated that VEGF-loaded HG-HA-TCP scaffold improved new bone regeneration and enhanced bone mineral density, revealed byhistological, micro-CT and histochemical straining analyses. Osteogenic and angiogenic abilities of the three biological scaffolds wereranked as follows: HG-HA-TCP (VEGF) > G-HA-TCP (VEGF) > G-HA-TCP. Conclusion: HG-HA-TCP (VEGF) scaffold with good biocompatibility could create an encouraging osteogenic microenvironment that could accelerate vessel formation and osteogenesis, providing an effective scaffold for bone tissue engineering and developing new clinical treatment strategies for bone tissue defects.

4.
J Mol Model ; 28(6): 147, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35556181

RESUMO

In this research, the interaction mechanism between H and CH3 of chemisorption on diamond (100)-2 × 1 surface was studied through the density functional theory (DFT) method. The H or CH3 adsorbates were assumed to be directly chemisorbed to the final position on the surface in thermodynamic studies. The adsorption energies of individual H and CH3 chemisorbed on the diamond surface were calculated, respectively. Subsequently, the adsorption energies for another H or CH3 in five different positions adjacent to the initial H or CH3 were calculated and compared. We find the universal law of the most likely chemisorption position. The results revealed that when one carbon atom of the dimer chemisorbed one radical, the other carbon atom of the dimer generated a dangling bond and had more ability to chemisorb other radicals. Therefore, the growth rate is faster along the direction of the carbon dimer than in other directions during the growth of the diamond film. The dimer is exactly towards the [110] direction. As films thicken, diamond tends to grow along the [110] direction. Therefore, it could explain that the [110] texture appears easily in the experiment. And, the results can be used to analyze the detailed process relative to the growth of the diamond film.

5.
Genes (Basel) ; 13(4)2022 03 30.
Artigo em Inglês | MEDLINE | ID: mdl-35456426

RESUMO

Lactic acid was previously considered a waste product of glycolysis, and has now become a key metabolite for cancer development, maintenance and metastasis. So far, numerous studies have confirmed that tumor lactic acid levels are associated with increased metastasis, tumor recurrence and poor prognosis. However, the prognostic value of lactic acid metabolism and transporter related genes in patients with clear cell renal cell carcinoma has not been explored. We selected lactic acid metabolism and transporter related twenty-one genes for LASSO cox regression analysis in the E-MTAB-1980 cohort, and finally screened three genes (PNKD, SLC16A8, SLC5A8) to construct a clinical prognostic model for patients with clear cell renal cell carcinoma. Based on the prognostic model we constructed, the over survival (hazard ratio = 4.117, 95% CI: 1.810-9.362, p < 0.0001) of patients in the high-risk group and the low-risk group in the training set E-MTAB-1980 cohort had significant differences, and similar results (hazard ratio = 1.909, 95% CI: 1.414-2.579 p < 0.0001) were also observed in the validation set TGCA cohort. Using the CIBERSORT algorithm to analyze the differences in immune cell infiltration in different risk groups, we found that dendritic cells, M1 macrophages, and CD4+ memory cells in the high-risk group were significantly lower than those in the low-risk group, while Treg cells were higher than in the low-risk group. Finally, through gene enrichment analysis, we found that the signal pathway that is strongly related to the prognostic model is the cell cycle.


Assuntos
Carcinoma de Células Renais , Neoplasias Renais , Biomarcadores Tumorais/genética , Carcinoma de Células Renais/patologia , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Renais/patologia , Ácido Láctico , Masculino , Transportadores de Ácidos Monocarboxílicos/genética , Transportadores de Ácidos Monocarboxílicos/metabolismo , Recidiva Local de Neoplasia/genética , Prognóstico
6.
Sci Total Environ ; 833: 155153, 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35413343

RESUMO

Fire plays an important role in carbon emissions and climate changes in the Earth system and global/regional fires in the past have been detected. However, palaeofire history and their potential drivers are poorly understood in Central Asia. We in this study synthesized existing sedimentary charcoal records to reconstruct Holocene fire history in Central Asia and then assessed the relative importance of two potential controls (climatic and anthropogenic factors) on its trends. The results are: (1) Fire activity had insignificantly increased since ~7.9 cal. yr BP in Central Asia, although there was an obvious difference when fire history at different elevations is considered; (2) Periods of high fire activity occurred in the early-middle Holocene following by a decreasing trend in the late Holocene at high elevation, which might be attributed to a cooling temperature and the cooling-caused low biomass burning in the Holocene interval; (3) Episodes of high fire activity were common in the late Holocene and were primarily driven by the wetting-induced increased biomass burning, reinforced by the intensified human activities in middle-low elevation. Our findings could not only provide new unique insight about the long-time-scale fire-human-climate-vegetation interactions and also provide reasonable guidance for fire management given current climate change in Central Asia.


Assuntos
Ecossistema , Incêndios , Ásia , Biomassa , Mudança Climática , Humanos
7.
Stem Cells ; 40(3): 290-302, 2022 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-35356984

RESUMO

Cellular senescence severely limits the research and the application of dental pulp stem cells (DPSCs). A previous study conducted by our research group revealed a close implication of ROR2 in DPSC senescence, although the mechanism underlying the regulation of ROR2 in DPSCs remains poorly understood so far. In the present study, it was revealed that the expression of the ROR2-interacting transcription factor MSX2 was increased in aging DPSCs. It was demonstrated that the depletion of MSX2 inhibits the senescence of DPSCs and restores their self-renewal capacity, and the simultaneous overexpression of ROR2 enhanced this effect. Moreover, MSX2 knockdown suppressed the transcription of NOP2/Sun domain family member 2 (NSUN2), which regulates the expression of p21 by binding to and causing the 5-methylcytidine methylation of the 3'- untranslated region of p21 mRNA. Interestingly, ROR2 downregulation elevated the levels of MSX2 protein, and not the MSX2 mRNA expression, by reducing the phosphorylation level of MSX2 and inhibiting the RNF34-mediated MSX2 ubiquitination degradation. The results of the present study demonstrated the vital role of the ROR2/MSX2/NSUN2 axis in the regulation of DPSC senescence, thereby revealing a potential target for antagonizing DPSC aging.


Assuntos
Senescência Celular , Polpa Dentária , Senescência Celular/genética , Polpa Dentária/metabolismo , Regulação para Baixo/genética , Regulação da Expressão Gênica , RNA Mensageiro/genética
8.
Micromachines (Basel) ; 13(2)2022 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-35208351

RESUMO

Graphene has been widely used due to its excellent electrical, mechanical and chemical properties. Defects produced during its transfer process will seriously affect the performance of graphene devices. In this paper, single-layer graphene was transferred onto glass and silicon dioxide (SiO2) substrates by wet transfer technology, and the square resistances thereof were tested. Due to the different binding forces of the transferred graphene surfaces, there may have been pollutants present. PMMA residues, graphene laminations and other defects that occurred in the wet transfer process were analyzed by X-ray photoelectron spectroscopy and Raman spectroscopy. These defects influenced the square resistance of the produced graphene films, and of these defects, PMMA residue was the most influential; square resistance increased with increasing PMMA residue.

9.
J Food Sci ; 87(3): 1184-1196, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35122248

RESUMO

Microencapsulated procyanidins by extruding starch (MPS) were used in meat and meat products as an antioxidant for their simple production process and high stability. This study investigated the controlled released properties of MPS and their effect on antioxidant capacity, physicochemical properties, and sensory qualities of chicken sausages during 4°C storage within 28 days. Antioxidant capacity, particle size analysis, and simulated digestion in vitro demonstrated that microencapsulation by extruding starch delayed the procyanidins release. The reduced crystal structure of MPS was determined by the morphology observation (SEM) and the decrease of the typical diffraction peak at 2θ of 20.9° (XRD). The MPS-added sausage had a higher (p < 0.05) ABTS and DPPH radical scavenging ratio (97.6% and 67.3%) and sulfhydryl contents (114.69 nmol/g protein) than other groups. Moreover, lower (p < 0.05) thiobarbituric acid reactive substances (TBARS) (0.67 mg MDA/kg sausage) and carbonyl values (3.24 nmol/mg protein) were detected in MPS-added sausages than others at the end of storage. The MPS addition increased redness (a* value) and decreased the lightness (L* value). The sensory analysis suggested that the sausage with the increased redness was favorable. These results denominated that MPS was an alternative antioxidant in chicken sausages. Practical Application: In this study, microencapsulated procyanidins were prepared by extrusion technology, and the effect on the quality of chicken sausages was investigated, which provides an alternative natural antioxidant for meat and meat products.


Assuntos
Produtos da Carne , Proantocianidinas , Animais , Galinhas , Lipídeos/química , Produtos da Carne/análise , Oxidantes , Amido
10.
J Nanobiotechnology ; 20(1): 3, 2022 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-34983531

RESUMO

BACKGROUND AND AIMS: Renal fibrosis is the common outcome in all progressive forms of chronic kidney disease. Unfortunately, the pathogenesis of renal fibrosis remains largely unexplored, among which metabolic reprogramming plays an extremely crucial role in the evolution of renal fibrosis. Ceria nanoparticles (CeNP-PEG) with strong ROS scavenging and anti-inflammatory activities have been applied for mitochondrial oxidative stress and inflammatory diseases. The present study aims to determine whether CeNP-PEG has therapeutic value for renal fibrosis. METHODS: The unilateral ureteral obstructive fibrosis model was used to assess the therapeutic effects in vivo. Transforming growth factor beta1-induced epithelial-to-mesenchymal transition in HK-2 cells was used as the in vitro cell model. The seahorse bioscience X96 extracellular flux analyzer was used to measure the oxygen consumption rate and extracellular acidification rate. RESULTS: In the present study, CeNP-PEG treatment significantly ameliorated renal fibrosis by increased E-cadherin protein expression, and decreased α-SMA, Vimentin and Fibronectin expression both in vitro and in vivo. Additionally, CeNP-PEG significantly reduced the ROS formation and improved the levels of mitochondrial ATP. The seahorse analyzer assay demonstrated that the extracellular acidification rate markedly decreased, whereas the oxygen consumption rate markedly increased, in the presence of CeNP-PEG. Furthermore, the mitochondrial membrane potential markedly enhanced, hexokinase 1 and hexokinase 2 expression significantly decreased after treatment with CeNP-PEG. CONCLUSIONS: CeNP-PEG can block the dysregulated metabolic status and exert protective function on renal fibrosis. This may provide another therapeutic option for renal fibrosis.


Assuntos
Cério , Glicólise/efeitos dos fármacos , Rim , Nanopartículas Metálicas/química , Fosforilação Oxidativa/efeitos dos fármacos , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Linhagem Celular , Cério/química , Cério/farmacologia , Fibrose/metabolismo , Humanos , Rim/efeitos dos fármacos , Rim/metabolismo , Nefropatias/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL
11.
Cytokine ; 150: 155777, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34954494

RESUMO

Due to their susceptibility to several human viruses, the mink has been proposed as potential animal models for the study of human viral infections. However, there are no specific monoclonal antibody (mAbs) currently available for the detection of mink-specific interferon-gamma (miIFN-γ). The BALB/c mice were immunized intraperitoneally with purified recombinant miIFN-γ protein. The splenocytes were obtained and fused with murine myeloma cells. Five of 24 hybridoma clones were obtained to produce mAbs steadily with the strongest affinity to recombinant miIFN-γ protein. The isotype of the 31A, 31B and 31G were lgG 2b. The isotype of 44 and 46 were lgG 2a and 1. All five mAbs were κ light chains. Western blotting and indirect ELISA method showed that 5 mAbs were positive to miIFN-γ. Immunofluorescence showed that 2 mAbs (44 and 46) had a positive reaction to miIFN-γ. The hybridoma clone 46 had the highest sensitivity for the detection of miIFN-γ. Most importantly, our primary sandwich ELISA system (mAbs 46 and polyclonal antiserum) detected endogenous IFN-γ in mink lymphocytes infected with canine distemper virus (CDV). We have thus developed a novel mAbs could recognize miIFN-γ, and have demonstrated the first ELISA-based measurement of IFN-γ in lymphocyte of the mink.


Assuntos
Anticorpos Monoclonais , Vison , Animais , Ensaio de Imunoadsorção Enzimática/métodos , Hibridomas/metabolismo , Interferon gama/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Vison/metabolismo
12.
Nanomaterials (Basel) ; 11(11)2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34835668

RESUMO

Reflective imaging systems such as Cassegrain-type telescopes are widely utilized in astronomical observations. However, curved mirrors in traditional Cassegrain telescopes unavoidably make the imaging system bulky and costly. Recent developments in the field of metasurfaces provide an alternative way to construct optical systems, possessing the potential to make the whole system flat, compact and lightweight. In this work, we propose a design for a miniaturized Cassegrain telescope by replacing the curved primary and secondary mirrors with flat and ultrathin metasurfaces. The meta-atoms, consisting of SiO2 stripes on an Al film, provide high reflectance (>95%) and a complete phase coverage of 0~2π at the operational wavelength of 4 µm. The optical functionality of the metasurface Cassegrain telescope built with these meta-atoms was confirmed and studied with numerical simulations. Moreover, fabrication errors were mimicked by introducing random width errors to each meta-atom; their influence on the optical performance of the metasurface device was studied numerically. The concept of the metasurface Cassegrain telescope operating in the infrared wavelength range can be extended to terahertz (THz), microwave and even radio frequencies for real-world applications, where metasurfaces with a large aperture size are more easily obtained.

13.
J Transl Med ; 19(1): 477, 2021 11 25.
Artigo em Inglês | MEDLINE | ID: mdl-34823548

RESUMO

Obesity is associated with chronic low-grade inflammation, contributing to an increasing prevalence of chronic metabolic diseases, such as insulin resistance, non-alcoholic fatty liver disease (NALFD), and steatohepatitis. Macrophages are the predominant immune cells in adipose tissues. Adipose tissue macrophages (ATMs) would switch to pro-inflammatory M1 state during obesity, causing local and systemic inflammation. However, the regulatory mechanism of ATMs has not yet been well described within this process. Using a high-fat diet (HFD)-induced mouse obesity model, we found that the costimulatory molecule CD226 was highly expressed on ATMs and knockout (KO) of CD226 alleviated obesity caused by HFD. Loss of CD226 reduced the accumulation of ATMs and hindered macrophage M1 polarization, with lower serum proinflammatory cytokine levels. Furthermore, deficiency of CD226 on ATMs decreased the phosphorylation levels of VAV1, AKT, and FOXO1 and thereby upregulated PPAR-γ. Further administration of PPAR-γ inhibitor restored M1 phenotype in CD226KO ATMs. In summary, loss of CD226 alleviates the HFD-induced obesity and systemic inflammation through inhibition of the accumulation and M1 polarization of ATMs in which PPAR-γ-dependent signaling pathway is involved, suggesting that CD226 may be identified as a potential molecular target for the clinical treatment of obesity.


Assuntos
Dieta Hiperlipídica , Resistência à Insulina , Tecido Adiposo , Animais , Dieta Hiperlipídica/efeitos adversos , Inflamação , Macrófagos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Obesidade/genética , Fenótipo
14.
Int J Gen Med ; 14: 8455-8461, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34824544

RESUMO

AIM: To analyze the movement of anterior teeth by changing the height of the power-arm and changing the force application points during whole maxillary dentition distalization with the aid of micro-implants in lingual orthodontics to set a biomechanical reference for effective clinical use of lingual orthodontic appliance. METHODS: A three-dimensional finite element model of the maxillary teeth with lingual appliance and the associated support tissue was established. Maxillary dentition with the force of 200g was distalized using implant as anchorage, then the movement of anterior teeth was analyzed by changing the length of power-arm (1mm, 3mm, 6mm, 9mm) and by changing the force location from lingual side to buccal side. RESULTS: During whole maxillary dentition distalization with aid of the implants in lingual orthodontics: when the height of power arm was 1mm, the anterior teeth rotated clockwise, with the increasing of the height of power-arm, the anterior teeth rotated counterclockwise gradually. When the height of power-arm was 9mm, all anterior teeth rotated counterclockwise. Central incisor and lateral incisor rotated counterclockwise and canine rotated clockwise when the buccal side force was applied. CONCLUSION: With the increase of the height of the power-arm, the movement pattern of the upper anterior teeth is different. The canine is more sensitive to the height of the power-arm than the central incisor and the lateral incisor. When the height of the power-arm reaches 9mm, the upper anterior teeth are displayed as crown tipping buccally movement. Compare with lingual side force, the buccal side force do better in preventing the loss of anterior tooth torque. If the upper anterior teeth are up-right or lingually tipped before treatment, it is preferable to use longer power-arm or buccal side traction force. If the anterior teeth are already tipped buccally, then short power-arm or lingual side force is advised.

15.
Chem Commun (Camb) ; 57(97): 13186-13189, 2021 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-34816269

RESUMO

A PGP-1-specific one/two-photon fluorogenic probe (BH1), capable of high sensitivity, super selectivity, and visual imaging of endogenous PGP-1 activity from live mammalian cells and serum/skin tissues from patients by using one/two-photon fluorescence microscopy (O/TPFM).


Assuntos
Corantes Fluorescentes/química , Inflamação/enzimologia , Fótons , Piroglutamil-Peptidase I/metabolismo , Animais , Linhagem Celular , Humanos , Inflamação/patologia , Camundongos , Microscopia de Fluorescência , Piroglutamil-Peptidase I/sangue
16.
Materials (Basel) ; 14(21)2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34772026

RESUMO

Flexible pressure sensors are widely used in different fields, especially in human motion, robot monitoring and medical treatment. Herein, a flexible pressure sensor consists of the flat top plate, and the microstructured bottom plate is developed. Both plates are made of polydimethylsiloxane (PDMS) by molding from the 3D printed template. The contact surfaces of the top and bottom plates are coated with a mixture of poly (3,4-ethylenedioxythiophene) poly (styrene sulfonate) (PEDOT:PSS) and polyurethane dispersion (PUD) as stretchable film electrodes with carbon nanotubes on the electrode surface. By employing 3D printing technology, using digital light processing (DLP), the fabrication of the sensor is low-cost and fast. The sensor models with different microstructures are first analyzed by the Finite Element Method (FEM), and then the models are fabricated and tested. The sensor with 5 × 5 hemispheres has a sensitivity of 3.54 × 10-3 S/kPa in the range of 0-22.2 kPa. The zero-temperature coefficient is -0.0064%FS/°C. The durability test is carried out for 2000 cycles, and it remains stable during the whole test. This work represents progress in flexible pressure sensing and demonstrates the advantages of 3D printing technology in sensor processing.

17.
Talanta ; 235: 122719, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34517587

RESUMO

Here, a dual lock-and-key fluorescence probe was developed for visualizing the inflammatory process in myocardial H9C2 cells. The probe possessed two-photon properties, viscosity sensitivity, and hydrogen peroxide (H2O2) responsiveness. A thiocarbamate spacer between fluorophore and H2O2 responsive unit enabled the release of carbonyl sulfide (COS). This rapidly converts to the anti-inflammatory hydrogen sulfide (H2S) by the ubiquitous enzyme carbon anhydrase. The probe displayed a dual response towards hydrogen peroxide and viscosity in vitro. No obvious fluorescence changes were observed towards either hydrogen peroxide or viscosity alone. In cellular experiments, the probe demonstrated good biocompatibility, low toxicity, and was shown responses towards exogenous and endogenous hydrogen peroxide under viscosity conditions. LPS induced cell inflammation showed it was able to effectively alleviate the inflammation-caused damage by releasing H2S and eliminating H2O2. The new protocol demonstrates its promising to achieve diagnosis and treatment of cellular inflammatory process.


Assuntos
Corantes Fluorescentes , Sulfeto de Hidrogênio , Fluorescência , Células HeLa , Humanos , Peróxido de Hidrogênio , Inflamação/tratamento farmacológico , Viscosidade
18.
Front Surg ; 8: 686629, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34447782

RESUMO

A 33-year-old female with a mild elevation of liver transaminase was sent to the general surgery department for medical services due to upper-right abdominal pain for 2 weeks. A liquid dark area ~4 × 3 × 3 cm in size in the theoretical location of the pancreatic segment of the common bile duct was detected by abdominal CT with no enhancement of the cystic wall found in the enhanced CT scan. The patient was then diagnosed with a choledochal cyst based on the results of the radiological images preoperatively. During the operation, the isolated cystic dilatation was found in the middle part of the cystic duct, and its caudal portion was found behind the head of the pancreas and converged into the common bile duct at an acute angle and low insertion. According to the intraoperative evaluation, the female was then diagnosed with a cystic duct cyst (CDC). The surgery was converted to a laparotomy for the unclear structure and the possibility of anatomic variation of the bile duct. The caudal portion of the cystic duct was found communicated with the common bile duct with a narrow base, and the extrahepatic bile duct was not cystic. The CDC was removed in the surgery. One week later, the patient was discharged from the hospital for the disappearance of abdominal pain and normal liver transaminase and did not report any discomfort in the 1-month-long follow-up. The lessons drawn from this case were as follows: (1) the distinction between the relatively frequent choledochal cyst and the isolated CDC should always be taken in mind; (2) a surgical strategy should be given priority for an intraoperatively confirmed CDC; (3) a common bile duct exploration is recommended for patients with choledocholithiasis or jaundice.

19.
Aging Cell ; 20(8): e13430, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34278704

RESUMO

Dental pulp stem cells (DPSCs) play a vital role in tooth restoration, regeneration, and homeostasis. The link between DPSC senescence and tooth aging has been well-recognized. ROR2 plays an important role in aging-related gene expression. However, the expression and function of ROR2 in DPSC aging remain largely unknown. In this study, we found that ROR2 expression was significantly decreased in aged pulp tissues and DPSCs. The depletion of ROR2 in young DPSCs inhibits their self-renewal capacity, while its overexpression in aged DPSCs restores their self-renewal capacity. Interestingly, we found that sphingomyelin (SM) is involved in the senescence of DPSCs regulated by ROR2. Mechanistically, we confirmed that ROR2 inhibited the phosphorylation of STK4, which promoted the translocation of Forkhead Box O1 (FOXO1) to the nucleus. STK4 inhibition or knockdown of FOXO1 markedly increased the proliferation of DPSCs and upregulated the expression of SMS1, which catalyzed SM biogenesis. Moreover, FOXO1 directly bound to the SMS1 promoter, repressing its transcription. Our findings demonstrated the critical role of the ROR2/STK4-FOXO1/SMS1 axis in the regulation of SM biogenesis and DPSC senescence, providing a novel target for antagonizing tooth aging.


Assuntos
Polpa Dentária/metabolismo , Proteína Forkhead Box O1/antagonistas & inibidores , Receptores Órfãos Semelhantes a Receptor Tirosina Quinase/metabolismo , Esfingomielinas/biossíntese , Células-Tronco/metabolismo , Regulação para Baixo , Humanos
20.
Front Genet ; 12: 688241, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34306029

RESUMO

BACKGROUND: The low-density lipoprotein receptor-related protein 6 (LRP6) gene is a recently defined gene that is associated with the autosomal-dominant inherited tooth agenesis (TA). In the present study, a family of four generations having TA was recruited and subjected to a series of clinical, genetic, in silico, and in vitro investigations. METHODS: After routine clinical evaluation, the proband was subjected to whole-exome sequencing (WES) to detect the diagnostic variant. Next, in silico structural and molecular dynamics (MD) analysis was conducted on the identified novel missense variant for predicting its intramolecular impact. Subsequently, an in vitro study was performed to further explore the effect of this variant on protein maturation and phosphorylation. RESULTS: WES identified a novel variant, designated as LRP6: c.2570G > A (p.R857H), harbored by six members of the concerned family, four of whom exhibited varied TA symptoms. The in silico analysis suggested that this novel variant could probably damage the Wnt bonding function of the LRP6 protein. The experimental study demonstrated that although this novel variant did not affect the LRP6 gene transcription, it caused a impairment in the maturation and phosphorylation of LRP6 protein, suggesting the possibility of the disruption of the Wnt signaling. CONCLUSION: The present study expanded the mutation spectrum of human TA in the LRP6 gene. The findings of the present study are insightful and conducive to understanding the functional significance of specific LRP6 variants.

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