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1.
Am J Mens Health ; 16(4): 15579883221113203, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35864746

RESUMO

This meta-analysis was to evaluate the efficacy and safety of holmium laser enucleation of prostate (HoLEP) in the treatment of benign prostatic hyperplasia (BPH) with large volume. PubMed, Embase, and Cochrane Library databases (until March 2022) were used to search related randomized controlled trials. A total of 11 studies including 1,258 patients were involved. HoLEP could significantly decrease the length of hospital stay and accelerate recovery. In subanalysis, HoLEP had better perioperative outcomes than bipolar transurethral resection of the prostate (B-TURP) and bipolar transurethral enucleation of the prostate (BPEP). The improvement in operative time and enucleation time was better in thulium laser enucleation of the prostate (ThuLEP) than HoLEP. In the follow-up period, the HoLEP decreased post-void residual urine (PVR) in short-term intervals and improved patients' maximum flow rate (Qmax) and prostate-specific antigen (PSA) in mid- and long-term intervals. In subanalysis, HoLEP presented significant improvements in Qmax, PSA, and quality of life (QoL) than B-TURP, and HoLEP could also improve Qmax than ThuLEP after 6 months of surgery. The HoLEP reduced the risk of postoperative bleeding compared with other surgeries in safety. In our study, we confirmed the advantages of HoLEP in treating BPH when the prostate size was larger than 80 mL, which indicated that HoLEP could be the best choice for treatment of large volume of prostate.


Assuntos
Lasers de Estado Sólido , Hiperplasia Prostática , Ressecção Transuretral da Próstata , Humanos , Lasers de Estado Sólido/uso terapêutico , Masculino , Próstata , Antígeno Prostático Específico , Hiperplasia Prostática/cirurgia , Qualidade de Vida , Resultado do Tratamento
3.
Front Pharmacol ; 13: 816995, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35833034

RESUMO

Objectives: We conducted meta-analysis to demonstrate the efficacy and safety of ketamine on postoperative catheter-related bladder discomfort (CRBD). Methods: A systematic search was performed through PubMed, Embase, and Cochrane Library to identify all randomized controlled trials that used ketamine in postoperative CRBD. This study was carried out by using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses. We used RevMan version 5.3.0. to analyze the data. Results: Five RCTs involving 414 patients were included in the analysis. The incidence and severity of postoperative CRBD were assessed at 0, 1, 2, and 6 h. According to our results of meta-analysis, ketamine reduced the incidence of postoperative CRBD at 2 h (RR 0.39; 95% CI, 0.21-0.71; p = 0.002, I2 = 40%) and 6 h (RR 0.29; 95% CI, 0.16-0.50; p < 0.0001, I2 = 0%) significantly; however, there were no statistical differences at 0 h (RR 0.81; 95% CI, 0.35-1.88; p = 0.62, I2 = 96%) and 1 h (RR 0.57; 95% CI, 0.13-2.54; p = 0.46, I2 = 97%). In two studies, we compared the incidence of moderate-to-severe CRBD between groups according to the scaling system (none, mild, moderate, and severe), and data are presented as numbers. Patients in the ketamine group showed a significantly lower severity of CRBD than those in the placebo group at 1 h (RR 0.09; 95% CI, 0.03-0.31; p = 0.0001) and 2 h (RR 0.06; 95% CI, 0.01-0.44; p = 0.005). In contrast, there were no meaningful differences between the two groups in the severity of CRBD at 0 h (RR 0.18; p = 0.84) or 6 h (RR 0.20; 95% CI, 0.03-1.59; p = 0.13). There were no meaningful differences on the rate of adverse events between the ketamine group and control group, mainly including postoperative nausea and vomiting (RR 1.24; 95% CI, 0.89-1.72; p = 0.21), diplopia (RR 3.00; 95% CI, 0.48-18.67; p = 0.24), and hallucination (RR 3.00; 95% CI, 0.32-28.24; p = 0.34). Conclusion: Our meta-analysis demonstrated that a sub-hypnotic dose of ketamine administration can reduce the incidence and severity of postoperative CRBD without causing evident side effects.

4.
Int J Stem Cells ; 2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35769056

RESUMO

Background and Objectives: The search for a suitable alternative for urethral defect is a challenge in the field of urethral tissue engineering. Induced pluripotent stem cells (iPSCs) possess multipotential for differentiation. The in vitro derivation of urothelial cells from mouse-iPSCs (miPSCs) has thus far not been reported. The purpose of this study was to establish an efficient and robust differentiation protocol for the differentiation of miPSCs into urothelial cells. Methods and Results: Our protocol made the visualization of differentiation processes of a 2-step approach possible. We firstly induced miPSCs into posterior definitive endoderm (DE) with glycogen synthase kinase-3ß (GSK3ß) inhibitor and Activin A. We investigated the optimal conditions for DE differentiation with GSK3ß inhibitor treatment by varying the treatment time and concentration. Differentiation into urothelial cells, was directed with all-trans retinoic acid (ATRA) and recombinant mouse fibroblast growth factor-10 (FGF-10). Specific markers expressed at each stage of differentiation were validated by flow cytometry, quantitative real-time polymerase chain reaction (qRT-PCR) assay, immunofluorescence staining, and western blotting Assay. The miPSC-derived urothelial cells were successfully in expressed urothelial cell marker genes, proteins, and normal microscopic architecture. Conclusions: We built a model of directed differentiation of miPSCs into urothelial cells, which may provide the evidence for a regenerative potential of miPSCs in preclinical animal studies.

5.
Langmuir ; 38(21): 6720-6730, 2022 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-35584361

RESUMO

Water flow in a nanoscale channel is known to be affected by strong water-wall interactions; as a result, the flow considerably deviates from the conventional continuum flow. Nanochannel with a sudden contraction or expansion is the most fundamental morphological nanostructure in many nanoporous systems such as shale matrix, mudrock, membrane, etc. However, the nanoconfinement effects of water flow in nanoporous systems and its effect on macroscopic flow behavior are still evolving research topics. In this work, our recently developed pore-scale lattice Boltzmann method (LBM) considering the nanoscale effects is extended to directly simulate water flow in nanoporous systems. The results show that the flow rate is dramatically decreased in hydrophobic nanopores because of additional flow resistances at the contraction and expansion junctions. This indicates that the bundle of capillary models or the permeability averaging method overestimates the water flow rate in nanoporous media if the contraction/expansion effects between different nanopores are ignored. This work highlights the importance of wettability of the nanochannel in the determination of dynamic water flow behaviors in the contraction/expansion nanosystem. Other important aspects, including velocity distribution, flow patterns, and vortex characteristics as well as pressure variation along the flow direction, are for the first time revealed and quantified. Large differences can be found comparing gas or larger-scale water flows through the same system. A new type of pressure variation curve along the axis of flow direction is found in the hydrophobic nanochannel with a sudden contraction/expansion. This work provides the fundamental understanding of water transport through the nanoscale system with contraction and expansion effects, giving implications to a wide range of industry applications.

6.
Comput Methods Biomech Biomed Engin ; 25(9): 961-970, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35620897

RESUMO

Nucleic acid testing (NAT) has been widely used in many fields such as medical diagnosis, food safety testing and forensic identification. However, it can only be carried out in professional laboratory because the test process is complicated and rigorous. In this paper, a nucleic acid amplification system based on polymerase chain reaction (PCR) was developed to meet the requirements of point-of-care testing (POCT) for nucleic acids. Firstly, the mechanical structure and electronic control system were designed and constructed. Secondly, an integral separation PID algorithm for temperature control and an intelligent temperature compensation method based on support vector regression (SVR) were proposed. Finally, temperature measurement and biological experiments were performed to prove the stability and availability of the nucleic acid amplification system. The results showed that the system achieved a rapid temperature change velocity of 4.5 °C/s, and the steady-state error was within ± 0.5 °C. The nucleic acids in samples of different concentrations were well amplified, the system can be used for quantitative detection of nucleic acid with the help of a fluorescence detection system, and has higher sensitivity than Tianlong PCR instrument.


Assuntos
Ácidos Nucleicos , Técnicas de Amplificação de Ácido Nucleico/métodos , Ácidos Nucleicos/análise , Ácidos Nucleicos/genética , Sistemas Automatizados de Assistência Junto ao Leito , Testes Imediatos
7.
Chem Commun (Camb) ; 58(39): 5837-5840, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35420089

RESUMO

Rh-catalyzed highly chemo- and enantioselective hydrogenation of chromone-2-carboxylic acids was successfully established for the first time, providing a wide range of enantiopure chromanone-2-carboxylic acids with excellent results (up to 97% yield and 99% ee) and high efficiency (up to 10 000 TON). The carboxylic group in the substrate was demonstrated to play a vital role and an enantio-induction mode was elucidated by DFT calculation. This hydrogenation protocol provided straightforward access to various bioactive chromanoids.


Assuntos
Ródio , Ácidos Carboxílicos , Catálise , Hidrogenação , Estereoisomerismo
8.
Sci Total Environ ; 829: 154610, 2022 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-35307438

RESUMO

The CO2 and CH4 fluxes across the water-air interface were determined in two groups of swimming crab (Portunus trituberculatus)-ridgetail white prawn (Exopalaemon carinicauda) polyculture ponds. One group of ponds with sediment improver application were referred to as SAPs, and the other group receiving no sediment improver were as NSPs. During the farming season, both the SAPs and NSPs acted as CO2 sinks and CH4 sources. The cumulative CO2-C fluxes from the SAPs and NSPs were -26.78 and -23.49 g m-2, respectively, and the cumulative CH4-C emissions from the SAPs and NSPs were 0.24 and 0.28 g m-2, respectively. CO2 fluxes were significantly related to net primary production and water pH, and CH4 fluxes were mainly regulated by water temperature during the farming season. The application of the oxidation-based sediment improver had a positive effect on reducing the CH4 emissions across the water-air interface but had no effect on CO2 fluxes. The sediment improver reduced the organic matter contents and improved the sediment pH and redox potential, which may have facilitated a decrease in CH4 production in the sediment. The CO2 produced through the oxidation of organic material in the sediment may have been absorbed by strong photosynthesis, resulting in a nonsignificant difference in CO2 fluxes between the SAPs and NSPs. The results indicated that the application of sediment improvers in coastal polyculture ponds can reduce carbon emissions, especially CH4 emissions, during the farming period and could help mitigate global warming with regard to the sustained-flux global warming potential (SGWP) and sustained-flux global cooling potential (SGCP) models over a 20-year time horizon. Future studies on the CO2 and CH4 production rates of the sediment and the related microbial community could improve our understanding of the effect mechanism of the application of sediment improvers on CO2 and CH4 emissions from mariculture ponds.


Assuntos
Braquiúros , Metano , Animais , Aquicultura , Dióxido de Carbono , Lagoas , Água
9.
Zhongguo Zhong Yao Za Zhi ; 47(6): 1546-1557, 2022 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-35347952

RESUMO

Zhachong Shisanwei Pills, composed of 13 Chinese medicinal materials, are used for treating the diseases such as hemiplegia, pain of muscles and bones, rheumatism, and joint pain. The chemical composition and pharmacodynamics of Zhachong Shisanwei Pills have not been reported. Ultra-performance liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS) was employed to quickly identify the chemical components of Zhachong Shisanwei Pills, which was performed with Shim-pack GIST C_(18) column(4.6 mm×150 mm, 5 µm). The gradient elution was conducted with methanol-0.05% acetic acid as the mobile phase. Electrospray ionization mass spectrometry(ESI-MS) was carried out in both positive and negative ion modes. The compounds were identidied based on accurate relative molecular weight, fragment ion species, and the MS data of reference substances and in literature. In conclusion, a total of 98 compounds were identified, including 19 organic acids, 36 flavonoids, 13 volatile oils, 8 tannins, 5 2-(2-phenylethyl)chromones, 5 amino acids, 3 sesquiterpenoids, 3 alkaloids, and 2 other compounds. This study characte-rized the chemical components of Zhachong Shisanwei Pills rapidly for the first time, laying a foundation for further research on the pharmacodynamic material basis and quality evaluation.


Assuntos
Medicamentos de Ervas Chinesas , Espectrometria de Massas em Tandem , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Medicamentos de Ervas Chinesas/química , Espectrometria de Massas por Ionização por Electrospray
10.
Clin Res Hepatol Gastroenterol ; 46(6): 101869, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35108656

RESUMO

BACKGROUND: Apatinib exhibits the synergistic effect with transarterial chemoembolization (TACE) though inhibiting the neoangiogenetic reaction caused by TACE. In this real-world study, we aimed to evaluate the efficacy and safety of TACE plus apatinib-combined therapy (ACT) in intermediate to advanced hepatocellular carcinoma (HCC) patients. METHODS: Data from 168 intermediate to advanced HCC patients who received TACE alone (N = 49) or TACE plus ACT (N = 119) were extracted. Besides, ACT was defined as apatinib with or without other therapy, such as arsenic trioxide, microwave ablation and radioactive seed implantation. RESULTS: In TACE plus ACT group, the median overall survival (OS) was 30 months (95% confidence interval (CI): 24-40 months) with 1-year, 3-year and 5-year OS rate of 84.0%, 41.2% and 21.5%, respectively. While in TACE group, the median OS was only 14 months (95%CI: 11-17 months) with 1-year, 3-year and 5-year OS rate of 55.1%, 18.4% and 16.1%, separately. By comparation, the OS was prolonged in TACE plus ACT group compared with TACE group (P<0.001). After adjusted by multivariate Cox's regression analysis, TACE plus ACT (vs. TACE) independently related to the longer OS (hazard ratio: 0.504, P = 0.001). In TACE plus ACT group, the most frequent adverse events included hand-foot syndrome (95.8%), hypertension (95.8%), fatigue (90.8%), albuminuria (85.7%), anorexia (79.0%), diarrhea (66.4%), myelosuppression (58.8%), nausea/vomiting (49.6%) and abdominal pain (39.5%), besides, no grade 4 adverse events and treatment-related death occurred. CONCLUSION: TACE plus ACT is a promising treatment choice for the intermediate to advanced HCC patients.


Assuntos
Antineoplásicos , Carcinoma Hepatocelular , Quimioembolização Terapêutica , Neoplasias Hepáticas , Antineoplásicos/efeitos adversos , Carcinoma Hepatocelular/patologia , Quimioembolização Terapêutica/efeitos adversos , Terapia Combinada , Humanos , Neoplasias Hepáticas/patologia , Piridinas , Estudos Retrospectivos , Resultado do Tratamento
11.
J Environ Manage ; 306: 114392, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-34998090

RESUMO

Bivalve shellfish aquaculture has been proposed to abate eutrophication and increase carbon sink in integrated multi-trophic aquaculture ecosystems. An individual growth model for razor clams Sinonovacula constricta in an integrated aquaculture pond of Portunus trituberculatus- Penaeus japonicus- S. constricta was constructed based on dynamic energy budget (DEB) theory after parameter measurement and model validation. Goodness-of-fit indices (R-squared, mean difference, and absolute and relative root mean square error) showed that the DEB model accurately reproduced razor clam growth. The growth performance evaluation of razor clams under different environmental conditions showed that warming climate and food shortages inhibited the razor clam growth. The quantification results of ecological service showed that individual razor clam have the potential for nutrient (nitrogen and phosphorus) removal and CO2 fixation, but exhibit a source of CO2 in individual month. The possible applications and contribution of this aquaculture model in China are also discussed, and the assessment results can provide important support for the low carbon bivalve integrated aquaculture.


Assuntos
Bivalves , Ecossistema , Animais , Aquicultura , Eutrofização , Alimentos Marinhos
12.
Sci Total Environ ; 815: 152904, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-34998772

RESUMO

The chemistry of dissolved organic matter (DOM) in soil determines its bioavailability and is crucial to soil nutrient cycling and vegetation restoration. However, the response of DOM to revegetation in degraded ecosystems is not well understood. This study analyzed soil chronosequences from semifixed (SF) sand in the Mu Us Desert, China that was afforested with arbor and shrub 23-54 years ago. The DOM organic composition and chemical properties were investigated by fluorescence excitation-emission matrix spectrophotometry and UV-visible spectroscopy. As the revegetation progressed, DOM concentration at 0-20 cm continually increased. In arbor land, the DOM was mainly influenced by dissolved organic carbon, and the average concentration significantly increased from 67.5 to 203.2 mg C•kg-1. In the shrubland, changes in DOM were mainly influenced by dissolved organic nitrogen, with the average concentration significantly increasing from 3.7 to 8.9 mg N•kg-1. Arbor afforestation significantly increased the proportion of hydrophobic humic-like components (C1), with a decline in bioactive tryptophan-like components (C3) and higher DOM molecular complexity (i.e., higher molecular weight, aromaticity, and degree of humification). However, in shrubland, the proportions of C1 and C3 were significantly lower than those in arbor forest soil, with DOM molecular complexity slightly increased, and DOM tended to become more bioavailable over time. Linear regression analysis indicated that DOM rich in carbon had a high molecular complexity, while DOM rich in nitrogen is more bioavailable. These findings reveal a dissimilar evolution in the DOM composition and chemistry in the two forest types throughout the SF sand revegetation period. The DOM under arbor forest was more stable and conducive to soil organic matter sequestration, while the DOM under shrub forest contained more bioactive substances and was more favorable for nutrient cycling.


Assuntos
Substâncias Húmicas , Solo , Ecossistema , Florestas , Substâncias Húmicas/análise , Espectrometria de Fluorescência
13.
Complex Intell Systems ; 8(3): 1929-1939, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34777962

RESUMO

Bone age assessment using hand-wrist X-ray images is fundamental when diagnosing growth disorders of a child or providing a more patient-specific treatment. However, as clinical procedures are a subjective assessment, the accuracy depends highly on the doctor's experience. Motivated by this, a deep learning-based computer-aided diagnosis method was proposed for performing bone age assessment. Inspired by clinical approaches and aimed to reduce expensive manual annotations, informative regions localization based on a complete unsupervised learning method was firstly performed and an image-processing pipeline was proposed. Subsequently, an image model with pre-trained weights as a backbone was utilized to enhance the reliability of prediction. The prediction head was implemented by a Multiple Layer Perceptron with one hidden layer. In compliance with clinical studies, gender information was an additional input to the prediction head by embedded into the feature vector calculated from the backbone model. After the experimental comparison study, the best results showed a mean absolute error of 6.2 months on the public RSNA dataset and 5.1 months on the additional dataset using MobileNetV3 as the backbone.

14.
IEEE Trans Neural Netw Learn Syst ; 33(2): 452-472, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34932487

RESUMO

Recently, single-particle cryo-electron microscopy (cryo-EM) has become an indispensable method for determining macromolecular structures at high resolution to deeply explore the relevant molecular mechanism. Its recent breakthrough is mainly because of the rapid advances in hardware and image processing algorithms, especially machine learning. As an essential support of single-particle cryo-EM, machine learning has powered many aspects of structure determination and greatly promoted its development. In this article, we provide a systematic review of the applications of machine learning in this field. Our review begins with a brief introduction of single-particle cryo-EM, followed by the specific tasks and challenges of its image processing. Then, focusing on the workflow of structure determination, we describe relevant machine learning algorithms and applications at different steps, including particle picking, 2-D clustering, 3-D reconstruction, and other steps. As different tasks exhibit distinct characteristics, we introduce the evaluation metrics for each task and summarize their dynamics of technology development. Finally, we discuss the open issues and potential trends in this promising field.

15.
Front Pharmacol ; 13: 837499, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35370654

RESUMO

Background: Programmed death 1 (PD-1) inhibitors-tislelizumab, toripalimab, camrelizumab, and sintilimab-are used for advanced urothelial carcinoma (UC) in China. To date, the efficacy and adverse events (AEs) of these PD-1 inhibitors have been poorly reported for advanced UC. Methods: We reviewed 118 patients treated with PD-1 inhibitors for advanced UC from July 2019 to October 2021 at Yantai Yuhuangding Hospital. Patient data were obtained from hospital records and telephone follow-ups. The safety and efficacy of PD-1 inhibitors were assessed by RESIST and Common Terminology Criteria for Adverse Events (version 4.0), respectively. Results: During a median follow-up period of 6 months, 112 patients (95%) experienced AEs; of these, 104 (88%) were grade 1-2 AEs, and 60 (51%) were grade 3-4 AEs. The most common AE was anemia, and no patients died as a result of treatment. A subanalysis according to treatment method (PD-1 inhibitor vs. PD-1 inhibitor plus chemotherapy) was performed. The incidence of grade 1-2 AEs was not different between the groups (85% vs. 94%), but combination therapy significantly increased grade 3-4 AEs (32% vs. 89%). Monotherapy and combination therapy also did not differ with regard to immune-related AEs of grades 1-2 (13% vs. 22%) or grades 3-4 (1% vs. 6%). In efficacy, complete response was not observed, but 33 patients (28%) had partial response, 30 (25%) had stable disease, and 47 had progressive disease (40%). The overall response and disease control rates were 28% and 53%, respectively. The preliminary efficacy of disease control was better with combination therapy versus monotherapy (78 vs. 43%). Conclusion: PD-1 inhibitors show promising tolerance and efficacy in advanced UC. PD-1 inhibitors combined with chemotherapy offered better disease control but had more grade 3-4 AEs. The clinical use of combination therapy warrants caution.

16.
Front Pharmacol ; 12: 710943, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34867321

RESUMO

Urothelial carcinoma (UC) occupies a high incidence among all the genitourinary malignancies. Immune checkpoint inhibitors (ICIs), as alternative treatments of metastatic urothelial carcinoma (mUC), have been applied in the treatment of mUC after chemotherapy failure, with comparable efficacy and safety. ICIs can enhance anti-tumor T cell reactivity and promote immune control over the cancerous cells by blocking cytotoxic T-lymphocyte antigen 4 (CTLA-4) or the combination of PD-1 and PD-L1. In the treatment of urothelial carcinoma, ICIs show obvious advantage and can enhance survival rates. However, their adverse effects are gradually manifested with increasing clinical applications. Therefore, we review the adverse effects and toxicity of ICIs in patients with UC, aiming to provide sound theoretical references and therapeutic strategies for their clinical application.

17.
Front Pharmacol ; 12: 756582, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34867373

RESUMO

Background and Objective: Over the past few years, mirabegron has been increasingly used as a therapeutic option for neurogenic lower urinary tract dysfunction. Here, we carried out a meta-analysis to investigate the efficacy and safety of mirabegron for the treatment of neurogenic lower urinary tract dysfunction. Methods: We used a range of databases to retrieve randomized controlled trials (RCTs) relating to mirabegron in patients with neurogenic lower urinary tract dysfunction: PubMed, Embase, and Cochrane Library; our strategy conformed to the PICOS (populations, interventions, comparators, outcomes, and study designs) strategy. Results: Our analyses involved four RCTs involving 245 patients. We found that mirabegron treatment resulted in a significant improvement in bladder compliance [mean difference (MD) = 19.53, 95% confidence interval (CI): 14.19 to 24.87, P < 0.00001], urinary incontinence episodes (MD = -0.78, 95% CI: -0.89 to -0.67, P < 0.00001) and Incontinence Quality of Life (I-QOL) (MD = 8.02, 95% CI: 3.20 to 12.84, P = 0.001). Significant differences were detected in terms of Patient Perception of Bladder Condition (PPBC) (MD = -0.54, 95% CI: -1.46 to 0.39, P = 0.26) and urinary urgency episodes (MD = -0.72, 95% CI: -3.1 to 1.66, P = 0.55). With regard to safety, there were no significant differences between mirabegron and control groups in terms of the incidence of drug-related adverse events [odds ratio (OR): 0.83, 95% CI: 0.43 to 1.59, P = 0.57], arrhythmias (OR: 1.27, 95% CI: 0.37 to 4.38, P = 0.70), hypertension (OR: 0.70, 95% CI: 0.13 to 3.82, P = 0.68), or post-voiding residual volume (MD: 1.62, 95% CI: -9.00 to 12.24, P = 0.77). Conclusion: Mirabegron is an efficacious and safe treatment for patients with neurogenic lower urinary tract dysfunction.

18.
Front Plant Sci ; 12: 766389, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34880890

RESUMO

In-depth genome characterization is still lacking for most of biofuel crops, especially for centromeres, which play a fundamental role during nuclear division and in the maintenance of genome stability. This study applied long-read sequencing technologies to assemble a highly contiguous genome for yellowhorn (Xanthoceras sorbifolium), an oil-producing tree, and conducted extensive comparative analyses to understand centromere structure and evolution, and fatty acid biosynthesis. We produced a reference-level genome of yellowhorn, ∼470 Mb in length with ∼95% of contigs anchored onto 15 chromosomes. Genome annotation identified 22,049 protein-coding genes and 65.7% of the genome sequence as repetitive elements. Long terminal repeat retrotransposons (LTR-RTs) account for ∼30% of the yellowhorn genome, which is maintained by a moderate birth rate and a low removal rate. We identified the centromeric regions on each chromosome and found enrichment of centromere-specific retrotransposons of LINE1 and Gypsy in these regions, which have evolved recently (∼0.7 MYA). We compared the genomes of three cultivars and found frequent inversions. We analyzed the transcriptomes from different tissues and identified the candidate genes involved in very-long-chain fatty acid biosynthesis and their expression profiles. Collinear block analysis showed that yellowhorn shared the gamma (γ) hexaploidy event with Vitis vinifera but did not undergo any further whole-genome duplication. This study provides excellent genomic resources for understanding centromere structure and evolution and for functional studies in this important oil-producing plant.

19.
Talanta ; 235: 122797, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34517655

RESUMO

As the outbreak of coronavirus disease 2019 (COVID-19), on-site molecular diagnosis is becoming increasingly important. In this study, a freeze-drying method was introduced for PCR reagents to meet the requirements of microfluidic molecular diagnosis. Using this method, PCR components were pre-mixed and freeze-dried as a bead, which could be transferred into microfluidic chips easily. As this bead only required reconstitution in water, operational steps of PCR were simplified, pipetting errors and errors associated with improper handling of wet reagents could also be reduced. In addition, 19 PCR mixes for different targets (including both RNA and DNA) detection were stable when stored at room temperature (18-25 °C) for 1-2 years and may be stored longer as activity monitoring remains ongoing. To shorten the stability testing time, accelerated stability testing at higher temperatures was proposed. The evaluation periods of the freeze-dried PCR mixes were shortened to less than one month when stored at 56 °C and 80 °C. When attempts were further tried to predict the shelf lives for freeze-dried PCR mixes, our findings challenged the classic view of the Q10 method as a prediction model for freeze-dried PCR mixes and confirmed for the first time that this prediction was influenced by different factors at varying degrees. These studies and findings are important for the development of molecular diagnosis at both central laboratories and resource-limited areas.


Assuntos
COVID-19 , Microfluídica , Humanos , Patologia Molecular , Reação em Cadeia da Polimerase , SARS-CoV-2 , Temperatura
20.
Ther Adv Urol ; 13: 17562872211032485, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34345251

RESUMO

AIM: This meta-analysis aimed to evaluate the efficacy of magnetic stimulation (MS) in treating female stress urinary incontinence (SUI) and providing an alternative treatment for patients who are unwilling to undergo surgery. METHODS: Randomized controlled trials (RCTs) that evaluated MS as a remedy for female SUI were retrieved from various electronic databases, including MEDLINE, EMBASE, and the Cochrane Controlled Trial Registry system. Moreover, reference lists for related papers were carefully screened for relevant studies. RESULTS: A total of six RCTs evaluating the effect of MS in treating female SUI were included in this study. Compared with the placebo group, the MS group exhibited higher quality-of-life scores [mean difference (MD) 0.59, 95% credibility interval (CI) 0.23-0.95; p = 0.001] and lower International Consultation on Incontinence Questionnaire scores (MD -3.93, 95% CI -5.85 to -2.01; p < 0.0001). Moreover, they exhibited a higher objective cure rate (odds ratio 8.49, 95% CI 3.08-23.37). In addition, MS treatment reduced the number of episodes of urinary incontinence (MD -1.42, 95% CI -2.24 to -0.59; p = 0.0007) and urine loss on pad test (MD -4.67, 95% CI -8.05 to -1.28; p = 0.007). There were no significant treatment-related adverse reactions. CONCLUSION: This study evaluated the efficacy and safety of MS in the treatment of female SUI. The results have important implications for patients who do not wish to undergo surgical therapy. We found that MS treatment for SUI has positive outcomes, however, future studies should aim at establishing the best protocol for optimizing the therapeutic effect.

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