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1.
J Nanosci Nanotechnol ; 21(2): 1293-1299, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33183475

RESUMO

Cancer is a serious threat to human health and longevity, and is an important cause of disease death. At present, cancer is mainly treated by surgery, radiotherapy, chemotherapy, etc. The existing various methods of treating tumors have their limitations. Although there are immune, genetic and other treatment methods, they are still immature. Therefore, tumor-targeted drug delivery systems have attracted more and more attention in cancer treatment. Targeted nano-drugs are selectively targeted to the tumor surface to achieve targeted drug delivery. New nano-drugs have created new hotspots in medical research. It could be a new strategy for treating cancer. Carboxymethyl chitosan (CMC) is formed by the carboxylation of chitosan. It has good water solubility and biodegradability, biocompatibility and antibacterial properties, so CMC is the best choice as a nanomaterial. Isorhamnetin (Iso) is an important anticancer drug. This article uses nanomedicine technology to construct CMC as a carrier, Iso as an antitumor drug, and using polydopamine (PDA) to modify the surface of the particles. Through in vitro and in vivo experiments, the Iso/CMC-PDA nanosphere Targeting and Growth Inhibition of Cervical Cancer Cells.

2.
BMC Genomics ; 21(1): 756, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33138772

RESUMO

BACKGROUND: Recent advances in single-cell RNA sequencing have allowed researchers to explore transcriptional function at a cellular level. In particular, single-cell RNA sequencing reveals that there exist clusters of cells with similar gene expression profiles, representing different transcriptional states. RESULTS: In this study, we present SCPPIN, a method for integrating single-cell RNA sequencing data with protein-protein interaction networks that detects active modules in cells of different transcriptional states. We achieve this by clustering RNA-sequencing data, identifying differentially expressed genes, constructing node-weighted protein-protein interaction networks, and finding the maximum-weight connected subgraphs with an exact Steiner-tree approach. As case studies, we investigate two RNA-sequencing data sets from human liver spheroids and human adipose tissue, respectively. With SCPPIN we expand the output of differential expressed genes analysis with information from protein interactions. We find that different transcriptional states have different subnetworks of the protein-protein interaction networks significantly enriched which represent biological pathways. In these pathways, SCPPIN identifies proteins that are not differentially expressed but have a crucial biological function (e.g., as receptors) and therefore reveals biology beyond a standard differential expressed gene analysis. CONCLUSIONS: The introduced SCPPIN method can be used to systematically analyse differentially expressed genes in single-cell RNA sequencing data by integrating it with protein interaction data. The detected modules that characterise each cluster help to identify and hypothesise a biological function associated to those cells. Our analysis suggests the participation of unexpected proteins in these pathways that are undetectable from the single-cell RNA sequencing data alone. The techniques described here are applicable to other organisms and tissues.

3.
Reprod Sci ; 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33237516

RESUMO

Hypoxia-inducible factor-1α (HIF-1α) participates in the regulation of spermatogenic function in rats with varicocele (VC), and the PI3K/Akt pathway plays an important role in it. In the present research, we applied the CRISPR/Cas9 gene editing technique to silence the HIF-1α gene of VC rat testis, to explore the effect of HIF-1α on apoptosis of spermatogenic cells in VC rats through the PI3K/Akt pathway. Sprague Dawley rats were randomly assigned to four groups, including the normal rat group (group N), VC model group (group V), VC + HIF-1α-lentivirus group (group H), and VC + luciferase-lentivirus group (group L). Apoptosis of spermatogenic cells in rat testis was tested by TUNEL Kit. The morphologic changes of seminiferous tubules were viewed by a light microscope. Expressions of VEGF, Akt, p-Akt, p70S6K, and p-p70S6K were detected by means of Western blot, immunofluorescence, or immunohistochemistry methods. One-way ANOVA was applied to analyze the diverseness between groups. Compared with group N, the distribution of germ cells was disordered, apoptosis of spermatogenic cells increased significantly, and the expression of VEGF, p-Akt, and p-p70S6K was also increased in group V. Compared with group V, the damage of seminiferous epithelium in group H was improved, and the arrangement of the seminiferous epithelium was almost orderly. Apoptosis of spermatogenic cells decreased significantly, and the expression of VEGF, p-Akt, and p-p70S6K protein was decreased (P < 0.05). There was no significant difference between group N and group H (P > 0.05).In conclusion, HIF-1α is regulated by hypoxia in rats with varicocele to regulate its downstream gene VEGF which regulates spermatogenesis, and the PI3K/Akt signaling pathway plays a regulatory role in this process.

4.
Sci Total Environ ; : 143239, 2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33158512

RESUMO

In this work, we developed an efficient pathway construction strategy, consisting of DNA assembler-assisted pathway assembly and counterselection system-based chromosomal integration, for the rapid and efficient integration of synthetic biodegradation pathways into the chromosome of Pseudomonas putida KT2440. Using this strategy, we created a novel degrader capable of complete mineralization of γ-hexachlorocyclohexane (γ-HCH) and 1,2,3-trichloropropane (TCP) by integrating γ-HCH and TCP biodegradation pathways into the chromosome of P. putida KT2440. Furthermore, the chromosomal integration efficiencies of γ-HCH and TCP biodegradation pathways were improved to 50% and 41.6% in P. putida KT2440, respectively, by the inactivation of a type I DNA restriction-modification system. The currently developed pathway construction strategy coupled with the mutant KTUΔhsdRMS will facilitate implantation of heterologous catabolic pathways into the chromosome for rapid evolution of the biodegradation capacity of P. putida. More importantly, the successful removal of γ-HCH (10 mg/kg soil) and TCP (0.2 mM) from soil and wastewater within 14 days, respectively, highlighted the potential of the novel degrader for in situ bioremediation of γ-HCH- and TCP-contaminated sites. Moreover, chromosomal integration of gfp made the degrader to be monitored easily during bioremediation. In the future, this strategy can be expanded to a broad range of bacterial species for widespread applications in bioremediation.

5.
Food Chem ; : 128475, 2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33168257

RESUMO

To solve the stability and oxidation issues of alpha-linolenic acid (ALA), this study focused on developing ALA nanoemulsion system (ALA-NE, oil-in-water) and evaluating the effect of environmental conditions on physical stability and the effect of antioxidants on oxidative stability. The physicochemical properties of nanoemulsions were measured at different conditions, including particle diameter, zeta potential, retention rate and peroxidation value (POV). The particle diameter increased significantly and the retention rate decreased after 25 days storage under the conditions of high temperature and metal ions. However, the influence of ionic strength, pH and light was insignificant. As an antioxidant, Vitamin E was more effective at retarding lipid oxidation of nanoemulsions than that of vitamin C. These results provided reference information in preparing effective and stable ALA-NE systems and enlarging the application fields.

6.
J Diabetes ; 2020 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-33249774

RESUMO

OBJECTIVE: To explore the impact of ferritin level on the disassociation of glycated hemoglobin A1c (HbA1c) and mean plasma glucose (MPG). RESEACH DESIGN AND METHODS: We used a 2012-2013 cross-sectional survey conducted in Pinggu district, Beijing including 3,095 Chinese participants aged 25-75 years. We categorized their glycemic status by interviewing for diagnosed diabetes and by measuring HbA1c, fasting plasma glucose (FPG) and 2-h post-load plasma glucose (2-hPPG). We fitted a multivariable regression model to explore the impact of ferritin on the association of HbA1c or glycated albumin (GA) and mean plasma glucose. RESULTS: 5.65% of participants were diagnosed as diabetes using HbA1c criteria, while 9.79% using OGTT criteria. Compared with male, female had significantly lower hemoglobin levels (159.82 ± 11.56 vs. 135.93 ± 12.62) and lower ferritin levels [113.00 (68.55, 185.50) vs. 33.40 (12.40, 70.13)]. Linear regression analysis performed in different groups classified by different diagnose criterion indicated that the correlation between MPG and HbA1c differs in different tertiles of ferritin (lowest vs. middle vs. highest: R2 = 0.507 vs. 0.645 vs. 0.687 in female; R2 = 0.415 vs. 0.715 vs. 0.615 in male), and the association between MPG and HbA1c diminished in the lowest tertile of ferritin. CONCLUSIONS: Ferritin level might affect the association between glucose and HbA1c, which should be taken into account when using HbA1c as a diagnosis criterion for diabetes and pre-diabetes. This article is protected by copyright. All rights reserved.

7.
Chemosphere ; : 128932, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-33220977

RESUMO

Biofertilizers can replace chemical fertilizers to promote the plant growth without causing any pollution. The study of hydrogen-oxidizing bacteria (HOB) enrichment as biofertilizers from mixed culture is scarce. Our recent study shows that biofertilizing HOB are successfully enriched in a short hydraulic retention time of 10 h. While, the mechanism is unknown. This study intentionally used a two-stage method to enrich biofertilizing HOB specifically with nitrate as nitrogen source in Stage 1 and then 1-aminocyclopropane-1-carboxylate (ACC) as nitrogen source in Stage 2. It was found Pseudomonas (34.46%, reported HOB) predominated in Stage 1, while Azospirillum (59.35%), Azoarcus (36%) were dominant genera and Azospirillum lipoferum strain DSM 1691 (50%), Azoarcus olearius strain DQS-4 (50%) were dominant species in Stage 2. The enriched HOB of Stage 2 showed ACC deaminase activity. Furthermore, they could also fix N2 and consume Ca3(PO4)2. Thus, the two-stage method can be used as a specific enrichment for HOB as biofertilizers, which extends the application of HOB in agriculture.

8.
Nutrients ; 12(11)2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33238490

RESUMO

Nutrition is an essential part of oncology care; however, nutrition advice and guidance are not always provided. This six-week pilot pretest-posttest intervention was designed to test the feasibility and effectiveness of integrating a nutrition education program (NutriCare) into outpatient oncology care. Twenty breast cancer survivors were recruited through Tufts Medical Centre. Nutrition impact symptoms and demographics were collected at baseline, dietary quality and quality of life measures were collected pre and post-intervention and an evaluation form was completed post-intervention. Forty-four percent of eligible participants were recruited, and 90% of those completed the study. The NutriCare program was well received with participants reporting that goals were feasible (94.4%), the program had a positive impact on their diet (77.8%), and over 80% would recommend the program. There was an interest in continuing with the program (89%) and in receiving additional guidance from the healthcare team (83%). There was a significant improvement (p = 0.04) in physical function over the six weeks; however, no additional significant differences in quality of life or dietary quality were seen. In conclusion, cancer survivors were positive about the NutriCare program and its integration into practice.

9.
Environ Pollut ; : 115831, 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33213947

RESUMO

The effects of algae on the removal of contaminant by iron sulfide (FeS) are still unknown. Chlorella vulgaris (CV), a remarkable algal specie, was used to prepare the CV-supported FeS (CV-FeS) and to investigate the role that CV plays in the removal of a heavy metal (i.e., hexavalent chromium (Cr(VI)) by FeS. The stabilized effect from algal extracellular polymeric substance (EPS) enhanced the reactivity of FeS due to the decrease of FeS aggregation, thus increasing Cr(VI) removal rate from 0.21 min-1 to 0.79 min-1. Furthermore, the strong buffering induced by the algal functional groups could effectively prevent the solution pH from increasing, which improved Cr(VI) removal because acidic solution facilitated Cr(VI) reduction by FeS. However, the complexing capacity from algal EPS made Fe(II) unavailable for Cr(VI) reduction, which led to 35% decrease of Cr(VI) removal. The Fe(II) was oxidized to α-FeOOH by Cr(VI) in the absence of CV, while the unreacted Fe(II) was detected as in the form of Fe(OH)2 in CV-FeS. Cr(VI) was reduced to Cr(III) and S(-II) was oxidized to elemental sulfur (S8) regardless of the CV. This work showed the different roles of algae in the removal of Cr(VI) by FeS and provided value information for the application of FeS in the polluted algae-containing water system.

10.
Am J Emerg Med ; 2020 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-33214018

RESUMO

INTRODUCTION: Thrombotic thrombocytopenic purpura (TTP) is a rare, life-threatening and easily misdiagnosed thrombotic microangiopathy disease. Few studies have reported the use of therapeutic plasma exchange (TPE) for TTP in emergency departments in China. The present study was a retrospective analysis of patients with TTP who were treated with TPE in our emergency intensive care unit (EICU). METHODS: This study retrospectively analyzed patients with TTP who received TPE management from July 1, 2014 to February 1, 2020. The following clinical data of these patients were collected: laboratory results, first symptoms, ADAMTS13 levels, glucocorticoid levels, TPE times and outcomes. RESULTS: The study included 19 patients (9 male and 10 female) with 20 clinical episodes, and 1 female patient had two episodes. TPE was used in 17 patients, and TPE was performed once every 2-3 days in patients. The volume for each TPE treatment was 2000 ml. In total, 4 male patients died, and 15 patients survived. One female experienced a relapse. No significant differences in age, RBC, HGB, PLT, ALT, AST, BUN, Cr, LDH, or bilirubin were noted between the survival and death groups. The mortality rate of male patients was significantly higher than that of female patients(p = 0.0325, p < 0.05), and the mean age of deceased patients was 64.25 ± 4.78 years, which was older than the mean age of survivors (47.38 ± 4.30). However, no significant difference was noted (p = 0.0787). CONCLUSION: TPE had satisfactory results for TTP patients although it was not performed every day. Older male TTP patients exhibited a relatively increased risk of death.

11.
Mikrochim Acta ; 187(12): 668, 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33215333

RESUMO

A chloramphenicol (CAP)-binding aptamer of 80 nucleotides (nt) was reported in 2011. In 2014, it was truncated to 40 nt and has since been used by most researchers, although a careful binding study is still lacking. In this work, binding assays using isothermal titration calorimetry and various DNA-staining dyes were performed. By comparing the truncated aptamer with three control sequences, no specific binding of CAP was observed in each case. The secondary structures of the original and truncated aptamers were analyzed, and it was shown that the likelihood of the truncated aptamer to retain the same binding mechanism as the original sequence is low. We further examined gold nanoparticle (AuNP)-based label-free colorimetric assays. By quantifying the extinction ratio at 620 nm over that at 520 nm, a similar color response was observed regardless of the sequence of DNA, suggesting the color change mainly reflected other events such as the adsorption of CAP by the AuNPs, instead of aptamer binding to CAP. Salt-induced aggregation experiments suggested direct adsorption of CAP on AuNPs. CAP only weakly inhibited DNA adsorption by AuNPs but did not displace pre-adsorbed DNA. Therefore, CAP adsorption by AuNPs needs to be considered when designing related sensors, for example, by using non-aptamer sequences as controls. This work calls for careful confirmation of aptamer binding and control experiments for designing aptamer and AuNP-based biosensors.

12.
Cancer Lett ; 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33152402

RESUMO

Immunoglobulin-like transcript (ILT) 3 is an immunosuppressive molecule that negatively regulates myeloid cell activation. ILT3 overexpression in tumor cells induces immune escape of solid tumors and facilitates invasion of monocytic acute myelocytic leukemia cells. However, the expression and function of ILT3 in non-small cell lung cancer (NSCLC) cells remain elusive. Herein, we found that ILT3 was enriched in human NSCLC cells, and predicted advanced disease and poor overall survival. ILT3 overexpression enhanced the migration and invasion of NSCLC cells and tubule formation of human umbilical vein endothelial cells by upregulating and interacting with its ligand apolipoprotein E (ApoE) in vitro. Mechanistically, ILT3 recruited SHP2 and SHIP1, and subsequently activated ERK1/2 signaling mediating epithelial-mesenchymal transition (EMT) and increasing vascular endothelial growth factor (VEGF)-A expression in NSCLC cells, which are responsible for tumor cell motility and angiogenesis, respectively. Using murine metastasis models, we further confirmed ILT3 promoted NSCLC metastasis and explored the exact correlation of ILT3 with ApoE, EMT, and VEGF-A in vivo. These results unraveled novel mechanisms for ILT3-induced tumor progression and proposed ILT3 as a potential therapeutic target and prognostic biomarker for NSCLC patients.

13.
Anal Chem ; 92(21): 14517-14527, 2020 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-33054169

RESUMO

Discovery of a new drug is time-consuming, laborious, and expensive. Herein, a novel integrative strategy for discovering potential new lead compounds has been developed, which was based on the characteristics of mass spectrometry (MS). MS was used to predict the potential forced degradation products (DPs) and metabolites of drugs by electrospray ionization and collision-induced dissociation (CID). Special rearrangement ions representing unique predicted DPs and metabolites were identified. The consistency between the predicted and the measured results was proven by in vitro metabolism and forced degradation of a commercial drug, respectively. From this, new chemical scaffold rearrangement ions named (aza)-biphenylenes, as potent anticancer agents, were discovered. As a representative aza-biphenylene analogue, 2-azabiphenylene was proven in vitro to induce apoptosis and inhibit the growth of various human cancer cells in a dose-dependent manner. Surprisingly, 2-azabiphenylene exhibited the best comparable bioactivity with the positive control sorafenib, but showed significantly lower in vitro cytotoxicity than sorafenib (at least a 5-fold decrease in cytotoxicity) because it could be targeted to the tumor microenvironment at low pH. A biradical mechanism accompanied by a mitochondrion-dependent oxidative stress mechanism was proposed to explore its anticancer mechanism. The highly reactive intermediate aza-biphenylenediyl worked as an active pharmaceutical ingredient and induced apoptosis of cancer cells. This provided the basis for the potential applications of CID-induced special rearrangement ions in developing new lead compounds.

14.
J Acad Nutr Diet ; 2020 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-33109503

RESUMO

BACKGROUND: Despite being motivated to improve nutrition and physical activity behaviors, cancer survivors are still burdened by suboptimal dietary intake and low levels of physical activity. OBJECTIVE: The aim of this study was to assess changes in nutrition and physical activity behaviors after cancer diagnosis or treatment, barriers to eating a healthy diet and staying physically active, and sources for seeking nutrition advice reported by breast cancer survivors. DESIGN: This was a cross-sectional study. PARTICIPANTS/SETTING: The study included 315 survivors of breast cancer who were recruited through social media and provided completed responses to an online exploratory survey. MAIN OUTCOME MEASURES: Self-reported changes in nutrition and physical activity behaviors after cancer diagnosis or treatment, perceived barriers to healthy eating and physical activity, and sources of nutrition advice were measured. STATISTICAL ANALYSIS: Frequency distribution of nutrition and physical activity behaviors and changes, barriers to healthy eating and physical activity, and sources of nutrition advice were estimated. RESULTS: About 84.4% of the breast cancer survivors reported at least 1 positive behavior for improving nutrition and physical activity after cancer diagnosis or treatment. Fatigue was the top barrier to both making healthy food choices (72.1%) and staying physically active (65.7%), followed by stress (69.5%) and treatment-related changes in eating habits (eg, change in tastes, loss of appetite, and craving unhealthy food) (31.4% to 48.6%) as barriers to healthy eating, and pain or discomfort (53.7%) as barriers to being physically active. Internet search (74.9%) was the primary source for seeking nutrition advice. Fewer than half reported seeking nutrition advice from health care providers. CONCLUSIONS: Despite making positive changes in nutrition and physical activity behaviors after cancer diagnosis or treatment, breast cancer survivors experience treatment-related barriers to eating a healthy diet and staying physically active. Our results reinforce the need for developing tailored intervention programs and integrating nutrition into oncology care.

15.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 2833-2836, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018596

RESUMO

Seizure types and characteristics may vary with time in a patient with distinct mechanisms underlying the propagation of ictal activity. Similarly, we found that both focal and generalized seizures coexist in some pilocarpine-induced chronic temporal lobe epilepsy (TLE) rats. In different seizure patterns, mapping complex networks and analyzing epileptic characteristics involved in seizure propagation are likely to reflect seizure propagation mechanisms, and indicate the establishment of stimulation strategy for epilepsy treatment, especially on the selection of stimulation targets. In our study, we used Granger causality method to track the time-variant epileptic brain functional connectivity in focal and generalized seizures from multi-site local field potentials (LFPs). Results showed that these two major types of seizures had different propagation patterns during ictal period. When comparing them, generalized seizures involved in a network with more complex relationships and spread to more extensive brain regions than in local seizures at mid-ictal stage. Moreover, we observed that focal seizures had a focused causal hub with strong interactions, while generalized seizures had relative distributed causal hubs to drive the development of seizure during seizure-onset stage. These findings suggest that stimulation strategy might need to be adapted to different seizure types thus allowing for retuning abnormal epileptic brain network and obtaining better treatment effect on seizure suppression.


Assuntos
Epilepsia do Lobo Temporal , Epilepsia , Animais , Encéfalo , Humanos , Pilocarpina , Ratos , Convulsões
16.
Accid Anal Prev ; 148: 105821, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33120183

RESUMO

This study applies a simulation-based traffic conflict technique to evaluate the hypothesis that sun glare under upper vents exerts negative impacts on traffic safety in urban tunnels. A modified cellular automata (CA) model is applied to simulate the deceleration behavior due to sun glare (DBSG) in real traffic. And the model is calibrated and validated against the empirical data. Conflict occurrences are generated through simulating vehicular interactions based on this model. Simulation experiments are conducted with different density and illuminance to evaluate the safety impacts of sun glare. Comparison of simulated conflict occurrences shows that rear-end conflicts occur more frequently as illuminance and density get higher. And the impacts of sun glare are more obvious on weak conflicts in moderate-density flow and more severe conflicts in high-density flows, respectively. To alleviate the negative impacts of sun glare, a sunshade system is designed based on the quantitative results.

17.
Anal Chim Acta ; 1136: 62-71, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33081950

RESUMO

Ultrasonic extraction and nebulization in real-time/carbon fiber ionization mass spectrometry (UEN/CFI-MS) was developed to screen the synthetic drugs adulterated into herbal products such as antidiabetic drug, antihypertensive drug, and hypolipidemic drug. Recently, ambient ionization MS techniques have achieved great advance for rapid analysis of sample surface. However, direct analysis of the analytes inside samples remains a challenge due to a lack of effective online sample extraction procedures. Owing to disappointing desorption efficiency, analytes inside the sample suffer from low detecting sensitivity when applying ambient ionization MS techniques. In this study, online ultrasonic extraction combined with carbon fiber ionization was used for real-time extraction, nebulization and detection of the analytes inside samples. The ultrasonic atomizer could produce a high-frequency vibration to realize online extraction and nebulization of sample. Then, the produced sample droplets could be immediately ionized by the carbon fiber ionization mass spectrometry. UEN/CFI-MS has shown great compatibility to solvents and compounds with a wide range of polarity and has few limitations for the shape of sample. UEN/CFI-MS was successfully applied for the rapid screening of synthetic drugs adulterated into herbal products. Among 37 batches of herbal products, 1 batch of Chinese patent medicine and 6 batches of dietary supplements were detected to be adulterated with the synthetic chemicals without labeling.

18.
Surg Obes Relat Dis ; 2020 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-33109444

RESUMO

BACKGROUND: Hiatal hernias are often repaired concurrently with bariatric surgery to reduce risk of gastroesophageal reflux disease-related complications. OBJECTIVES: To examine the association between concurrent hiatal hernia repair (HHR) and bariatric outcomes. SETTING: A 2010-2017 U.S. commercial insurance claims data set. METHODS: We conducted a retrospective cohort study. We identified adults who underwent sleeve gastrectomy (SG) or Roux-en-Y gastric bypass (RYGB) alone or had bariatric surgery concurrently with HHR. We matched patients with and without HHR and followed patients up to 3 years for incident abdominal operative interventions, bariatric revisions/conversions, and endoscopy. Time to first event for each outcome was compared using multivariable Cox proportional hazards modeling. RESULTS: We matched 1546 SG patients with HHR to 3170 SG patients without HHR, and we matched 457 RYGB patients with HHR to 1156 RYGB patients without HHR. A total of 73% had a full year of postoperative enrollment. Patients who underwent concurrent SG and HHR were more likely to have additional abdominal operations (adjusted hazard ratio [aHR], 2.1; 95% CI, 1.5-3.1) and endoscopies (aHR, 1.5; 95% CI, 1.2-1.8) but not bariatric revisions/conversions (aHR, 1.7; 95% CI, .6-4.6) by 1 year after surgery, a pattern maintained at 3 years of follow-up. Among RYGB patients, concurrent HHR was associated only with an increased risk of endoscopy (aHR, 1.4; 95% CI, 1.1-1.8)) at 1 year of follow-up, persisting at 3 years. CONCLUSIONS: Concurrent SG/HHR was associated with increased risk of some subsequent operative and nonoperative interventions, a pattern that was not consistently observed for RYGB. Additional studies could examine whether changes to concurrent HHR technique could reduce risk.

19.
Chem Commun (Camb) ; 56(90): 14075-14078, 2020 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-33107534

RESUMO

Thioredoxin reductase (TrxR) enzymes are critical in regulating redox homeostasis in cells. We report the first two-photon fluorescent probe of mammalian TrxR (TP-TRFS). TP-TRFS retains high specificity in recognizing TrxR. More importantly, the two-photon absorbing character of TP-TRFS enables it to be used in vivo. With the aid of TP-TRFS, a remarkable decline of the TrxR function was observed in the brain of a mouse model of stroke for the first time, providing a mechanistic link of TrxR dysfunction with stroke.

20.
Colloids Surf B Biointerfaces ; 197: 111385, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33049660

RESUMO

Biofilm infections pose a rising threat to public health due to its existing protective shield, which preventing biocide penetration. Here, the oleate-capped iron oxide nanoparticles (OIONPs) were synthesized by the high-temperature method first; after then, the poly(acrylic acid)-capped iron oxide nanoparticles (PIONPs) were obtained via a ligand exchange reaction between OIONPs and sodium poly(acrylic acid). The physicochemical properties of PIONPs were evaluated by Fourier-transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Scanning Transmission Electron Microscopy (STEM), Dynamic Light Scattering (DLS), and zeta potential. The FT-IR analysis confirmed the successful ligand exchange on the surface of iron oxide nanoparticles. STEM images displayed that the prepared PIONPs were monodisperse spherical nanoparticles. The PIONPs were stable in ultrapure water and could be kept for 5 weeks without aggregation. Next, Cell Counting Kit-8 (CCK-8) assay and fluorescent images confirmed the excellent cytocompatibility of PIONPs, while the iron concentration of PIONPs was in the range of 5∼120 mg/L. Finally, PIONPs exhibited efficient antibacterial activity against E. coli and S. aureus, and Staphylococcus aureus subsp. aureus Rosenbach (SASAR) biofilm could be destroyed by treating PIONPs under alternating current (AC) applied field conditions.

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