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1.
J Oleo Sci ; 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32051356

RESUMO

Functional oils have broad application prospects in functional foods and beverages because of their rich beneficial ingredients and healthier intake. The small droplets of the nanoemulsion enhance the effective delivery, solubility and bioavailability of the various hydrophobic food components. This study used a mixed oil phase of green tea seed oil and fractionated coconut oil, compared the emulsifying properties of natural surfactants: Whey protein isolate, soy lecithin, tea saponin and synthetic surfactant: Tween 80 in the preparation of nanoemulsions by ultrasonic method. In particular, the impact of emulsifier type and concentration, pH, ionic strength, and heat treatment on the mean particle size and ξ- potential were investigated. The long-term storage stability of the fabricated nanoemulsions was also monitored during storage at different temperatures. In addition, the effects of emulsifier type on the bioavailability of nanoemulsions were evaluated. For all nanoemulsions studied, the mean particle size decreasing with increasing emulsifier concentration. Tea saponin and soy lecithin can produce smaller droplets of nanoemulsion than Whey protein isolate. Tea saponin has the same emulsifying ability as Tween 80. Presumably tea saponin-stabilized droplets may be maintained by electrostatic repulsion and steric repulsion. All of the nanoemulsions significantly improved the bioavailability of the mixed oil phase compared to the unemulsified oil phase. This study highlights the potential of natural surfactants in the ultrasonic preparation of nanoemulsions containing functional oils, and provides a basis for the application of natural surfactants and new functional oils in food industry.

2.
Inorg Chem ; 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32052961

RESUMO

The alkali-metal molybdate iodate Na3(MoO4)(IO3) (I) and mixed-alkali-metal fluoromolybdate Na3Cs(MoO2F4)2 (II) were obtained via a mild hydrothermal reaction using a "Teflon-pouch" method. I crystallizes in the triclinic space group P1, whose structure comprises a 3D backbone made up of isolated [IO3]- pyramids and [MoO4]2- tetrahedra connected via 5- and 6-fold coordinated sodium cations. II crystallizes in the monoclinic space group P21/c and comprises isolated [MoO2F4]2- octahedra with strong out-of-center distortions and the Na+ as well as Cs+ cations acting as interstitial ions. Both compounds have been characterized by infrared (IR) spectra and ultraviolet-visible-near-infrared (UV-vis-NIR) diffuse reflectance spectra. First-principles calculations respectively reveal that they exhibit birefringence values with Δn = 0.078 and 0.210 at 1064 nm for I and II, and the origin of the birefringence is discussed.

3.
Cancer Sci ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31943575

RESUMO

Upstream ORF (uORF) is a translational initiation element located in the 5'UTR of eukaryotic mRNAs. Studies have found that uORFs play an important regulatory role in many diseases. Based on The Cancer Genome Atlas database, the results of our experiments and previous research evidence, we investigated transcription factor AP-4 (TFAP4) and its uORF, LIM and SH3 protein 1 (LASP1), long noncoding RNA 00520 (LINC00520), and microRNA (miR)-520f-3p as candidates involved in glioma malignancy, which is a poorly understood process. Both TFAP4-66aa-uORF and miR-520f-3p were downregulated, and TFAP4, LASP1, and LINC00520 were highly expressed in glioma tissues and cells. TFAP4-66aa-uORF or miR-520f-3p overexpression or TFAP4, LASP1, or LINC00520 knockdown inhibited glioma cell proliferation, migration, and invasion, but promoted apoptosis. TFAP4-66aa-uORF inhibited the translation of TFAP4 by binding to the TFAP4 mRNA. MicroRNA-520f-3p inhibited TFAP4 expression by binding to its 3'UTR. However, LINC00520 could promote the expression of TFAP4 by competitively binding to miR-520f-3p. In addition, TFAP4 transcriptionally activated LASP1 and LINC00520 expression by binding to their promoter regions, forming a positive feedback loop of TFAP4/LINC00520/miR-520f-3p. Our findings together indicated that TFAP4-66aa-uORF inhibited the TFAP4/LINC00520/miR-520f-3p feedback loop by directly inhibiting TFAP4 expression, subsequently leading to inhibition of glioma malignancy. This provides a basis for developing new therapeutic approaches for glioma treatment.

4.
J Med Virol ; 92(3): 356-363, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31663617

RESUMO

Gansu province is a region with the highest gastric cancer incidence and mortality in Northwest China. Epstein-Barr virus-associated gastric carcinoma (EBVaGC) is an important subtype of gastric cancer which shows specific clinicopathological features such as older-age bias, male predominance, lower lymph-node-metastasis, and a better cancer-related survival comparing to EBV-negative gastric cancers. However, the prevalence of EBVaGC has never been studied in Gansu Province, Northwest China. The present study investigated the incidence, characteristics, and EBV messenger RNA (mRNA) profile of EBVaGC in this area. We have collected 270 stomach samples from gastric cancer patients and analyzed the presence of EBV DNA and EBV-encoded small RNAs (EBERs) by nested polymerase chain reaction (PCR) and in situ hybridization, respectively. The EBV mRNA profiling was investigated by quantitative reverse transcription PCR (qRT-PCR). EBV DNA was detected in 51/95 patients (53.7%), while EBER transcripts were detected in 18/270 patients (6.7%). EBER positivity was significantly associated with older age and less lymph node metastasis, but no obvious association with gender or histological type of tumors. The expression of EBV genes was observed with different patterns, and the mRNA of glycoprotein BMRF2 was detected in EBVaGC. The present study showed unique clinicopathological features and mRNA expression patterns of EBVaGC in Gansu Province, Northwest China, suggesting that geographic variation can contribute to new epidemiological features in EBVaGC. The transcript of glycoprotein BMRF2 was observed consistently in EBVaGC, which may serve as a biomarker and play a role in the pathogenesis of EBVaGC in Gansu Province, Northwest China.

5.
Mol Ther ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31813799

RESUMO

Studies have found that RNA-binding proteins (RBPs) and long non-coding RNAs (lncRNAs) are dysregulated and play an important regulatory role in the development of tumors. Based on The Cancer Genome Atlas (TCGA) database, our findings from experiments, and the evidence of previous studies, we screened DiGeorge syndrome critical region gene 8 (DGCR8), ZFAT antisense RNA 1 (ZFAT-AS1), and caudal type homeobox 2 (CDX2) as research candidates. In the present study, DGCR8 and CDX2 were highly expressed and ZFAT-AS1 was markedly downregulated in glioma tissues and cells. DGCR8 or CDX2 knockdown or ZFAT-AS1 overexpression suppressed glioma cell proliferation, migration, and invasion and facilitated apoptosis. DGCR8 might decrease ZFAT-AS1 expression by attenuating its stability in a manner of inducing its cleavage. Importantly, ZFAT-AS1 could inhibit CDX2 transcription by mediating the methylation of histone H3 on lysine 27 (H3K27me3) modification induced by PRC2 in the CDX2 promoter region. In addition, CDX2 transcriptionally activated DGCR8 expression by binding to its promoter regions, forming a positive feedback loop of DGCR8/ZFAT-AS1/CDX2. In conclusion, DGCR8/ZFAT-AS1 promotes CDX2 transcription in a PRC2 complex-dependent manner to facilitate the malignant biological behavior of glioma cells.

6.
J Orthop Surg Res ; 14(1): 439, 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31836021

RESUMO

BACKGROUND: A detailed understanding of the blood supply to the femoral head is required to plan the surgery in the femoral neck and head area. However, information about the blood vessel networks in the femoral head is inadequate. METHODS: The surface of the femoral neck of 100 dry cadaveric adult femur specimens was scanned using a 3D scanner. The scanning distance was 200 mm, precision 0.01 mm, and measuring point 0.04 mm. The images were acquired at a resolution of 1,310,000 pixels. Digital imaging data were recorded from the femoral neck surface. The diameters of the nutrient foramina of the superior, inferior and anterior retinacular arteries, and the ligamentum teres arteries were determined and divided into five groups. RESULTS: The mean cumulative cross-sectional area of the nutrient foramina was as follows: canals of the superior, inferior, anterior, and ligamentum retinacular arteries were 15.59 mm2, 3.63 mm2, 4.32 mm2, and 1.58 mm2, respectively. Next, we analyzed the canals of the superior, inferior, anterior and ligamentum retinacular arteries, respectively, via 3D scanner. We found that the canals of the superior retinacular arteries appear to supply more blood to the femoral head than the canals of the other three types of arteries. CONCLUSIONS: Our results demonstrated that surgeries of the femoral neck and femoral head will be improved with prior 3D scanning and lead to better outcomes in surgeries involving the hip area.

7.
Adv Sci (Weinh) ; 6(22): 1901679, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31763150

RESUMO

The conflict between beneficial layered structure for performances and layered growth habits in KBe2BO3F2 (KBBF) always restricts its practical applications. A beryllium-free KBBF family member, Zn2BO3(OH), is explored to feature the same topological layer with KBBF by replacing [BeO3F]5- with [ZnO3(OH)]5- and excellent UV performances. It exhibits a second harmonic generation response of about 1.5 × KH2PO4 with the UV cutoff edge of 204 nm. The birefringence of Zn2BO3(OH) in the visible region is about 0.067, which is larger than those of commercial UV crystals LiB3O5, CsB3O5, and CsLiB6O10. Additionally, it has excellent thermal and water-resistant stabilities. Owing to the removal of interlayer cations, Zn2BO3(OH) shows better growth habits than KBBF while achieving the balance between beneficial layered structure and layer tendency.

8.
J Mater Chem B ; 7(48): 7771-7775, 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31754675

RESUMO

A new class of aggregation-induced emission (AIE) fluorophores HAPHs with excellent two-photon properties is developed from styrylquinoline. Among them, the probe HAPH-1 exhibits good sensitivity towards pH and viscosity and has been successfully used for simultaneous pH and viscosity detection in living cells through two-photon microscopy.

9.
Int J Neurosci ; : 1-5, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31675909

RESUMO

Objective: Anterior funiculus lesion is uncommon in subacute combined degeneration of the spinal cord with few data available. Aim of the study was to describe a case with the rare manifestation and summarize existing literatures.Methods: We report a case of a 42-year-old woman with anterior and lateral funiculus lesions on cervicothoracic spine magnetic resonance imaging, who presented with unsteady gait, sensory level and weakness of lower limbs. Besides, we reviewed and analyzed literatures about subacute combined degeneration of the spinal cord with anterior funiculus lesions published during the past two decades.Results: The diagnosis of subacute combined degeneration of the spinal cord was considered due to her presence of low serum vitamin B12 levels, pernicious anemia and gastric carcinoid.Conclusion: Physicians should consider subacute combined degeneration of the spinal cord as a possible differential diagnosis when faced with atypical lesions distributed in the anterior funiculus.

10.
Biomater Sci ; 7(12): 5404-5413, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31633702

RESUMO

A polyester hernia patch has received extensive attention in mesh hernia repair. However, it is still a challenge to develop polyester-based implants with inherent antibacterial properties due to the lack of active functional groups. In this study, poly(butylene succinate-co-butylene aspartate) (PBSA) was constructed by introducing aspartic acid on a polybutylene succinate (PBS) polyester chain (PBSA). Antimicrobial treatment was conducted by grafting levofloxacin (Lv) on the surface of a PBSA polymer (PBSA-g-Lv). In vitro antibacterial test results showed that PBSA-g-Lv had sufficient local antimicrobiotic effects against Staphylococcus aureus and Escherichia coli and no side effect on L929 cells was observed. Furthermore, almost no change was observed in the thermodynamic properties of PBS and PBSA; in vivo tests demonstrated that this contact-active antibacterial PBSA-g-Lv nanofiber is a promising material to fulfill the dual functions of promoting tissue regeneration and preventing bacterial infection. The presented data confirmed that an antibiotic surface modification of PBSA polyesters was expected to be used as hernia repair materials.

11.
Exp Ther Med ; 18(4): 2648-2652, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31572513

RESUMO

Local anesthetic of ropivacaine was demonstrated to reduce the postoperative pain in elderly patients. This study investigated the pharmacokinetics of ropivacaine at different concentrations in elderly patients subjected to fascia iliaca compartment block. Forty patients with femoral neck fracture at American Society of Anesthesiologists (ASA) I-II status, undergoing fascia iliaca compartment block (FICB) were randomized to two groups receiving 0.7 ml/kg of solution containing 0.375% ropivacaine (group L) or 0.5% ropivacaine (group H). Samples of venous blood were obtained immediately at different time points after FICB, and the total and free plasma concentrations of ropivacaine were measured by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Statistical analysis was carried out using a pharmacokinetic calculation program (DAS 3.0). Visual Analogue Scale (VAS) scores were significantly decreased after FICB in both groups, and VAS score in group H was lower compared with group L. The total maximum plasma concentration (Cmax) and the free Cmax of ropivacaine in group H was higher than that in group L (P<0.05). The decrease of the total and free plasma concentration was operation time-dependent. Neither group showed signs of central nervous system and circulatory system toxicity. On the basis of these results, the concentrations of 0.375 and 0.5% ropivacaine held an efficiently analgesic effect for FICB, suggesting that ropivacaine can be employed in analgesic therapy. However, both concentrations have a potentially theoretical risk of local anesthetics poisoning, suggesting that a lower concentration may be a safer option for a single large volume of FICB.

12.
Opt Express ; 27(15): 20824-20836, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31510171

RESUMO

A gain-tailored Ge-free Yb/Ce codoped aluminosilicate fiber is fabricated by MCVD combined with solution doping technique. Through regulating the temperature in the tube and designing the solution doping process, the refractive index profile of this fiber is close to a step-index without any center dip. The laser performance of this fiber is proved through contrast experiments with conventional fiber in a kW-level MOPA setup. The gain-tailored fiber amplifier presents a beam quality of M2 ~1.43 at 1.2 kW. Its MI threshold is 1.25 kW, about 1.74 times as much as that of the conventional fiber amplifier. The laser slope efficiency of the gain-tailored fiber amplifier is 86.75%. Stabilized at an output power of 1.1 kW for 15 hours, the MI threshold does not decrease after this long-term operation, demonstrating a strong resistance to photodarkening effect. These results have confirmed that MCVD-fabricated gain-tailored Yb/Ce codoped aluminosilicate fibers have great potential in power scaling and output stability of high-power fiber lasers and amplifiers.

13.
Polymers (Basel) ; 11(8)2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31370210

RESUMO

Improving the electrocatalytic performance of oxygen evolution reaction (OER) is essential for oxygen-involved electrochemical devices, including water splitting and rechargeable metal-air batteries. In this work, we report that the OER performance of commercial catalysts of IrO2, Co3O4, and Pt-C can be improved by replacing the traditional Nafion® ionomer with newly synthesized copolymers consisting of protonated imidazolium moieties such as ion conductors and binders in electrodes. Specifically, such an improvement in OER performance for all the tested catalysts is more significant in basic and neutral environments than that under acidic conditions. We anticipate that the results will provide new ideas for the conceptual design of electrodes for oxygen-involved electrochemical devices.

14.
J Colloid Interface Sci ; 555: 214-223, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31382140

RESUMO

In this work, Bi2S3/MoS2/Bi2MoO6 hetero-structured porous films were fabricated via a facile anion exchange process using the as-prepared Bi2MoO6 nanoflake array film as substrate material. The formation of Bi2S3/MoS2/Bi2MoO6 ternary hetero-structured porous film is both thermodynamically controllable and reaction time dependent. Systematic experiments were done to investigate the products at each reaction stage and disclose the relationships between the composite components and reaction temperature and time. The study showed that the energy barrier need to be overpassed when MoS2 and Bi2S3 were simultaneously produced. The optimized Bi2S3/MoS2/Bi2MoO6 photoelectrode exhibited significantly higher photoelectrocatalytic efficiency than Bi2MoO6, binary Bi2S3/Bi2MoO6 and Bi2S3/MoS2 photoelectrodes. The remarkable degradation efficiency of the Bi2S3/MoS2/Bi2MoO6 photoelectrode comes from the synergy of high quality assembly and heterostructure interfaces between the three components. The optimized film assembly and stepwise band alignment in the ternary heterostructure composite contribute to visible light utilization, transport and separation of charge carriers, mass transport, and accessibility of active sites. The generated active species such as superoxide anions (O2-) and holes were detected to promote the decomposition of organic pollutants. The reasonable photoeletrocatalytic degradation mechanism was also proposed.

15.
Biotechnol Biofuels ; 12: 180, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31338122

RESUMO

Background: DNA assembly is an essential technique enabling metabolic engineering and synthetic biology. Combining novel DNA assembly technologies with rational metabolic engineering can facilitate the construction of microbial cell factories. Amino acids and derived biochemicals are important products in industrial biotechnology with wide application and huge markets. DNA assembly scenarios encountered in metabolic engineering for the construction of amino acid and related compound producers, such as design-build-test-learn cycles, construction of precise genetic circuits and repetitive DNA molecules, usually require for iterative, scarless and repetitive sequence assembly methods, respectively. Results: Restriction endonuclease (RE)-assisted strategies constitute one of the major categories of DNA assembly. Here, we developed a Type IIP and IIS RE-assisted method named PS-Brick that comprehensively takes advantage of the properties of PCR fragments and REs for iterative, seamless and repetitive sequence assembly. One round of PS-Brick reaction using purified plasmids and PCR fragments was accomplished within several hours, and transformation of the resultant reaction product from this PS-Brick assembly reaction exhibited high efficiency (104-105 CFUs/µg DNA) and high accuracy (~ 90%). An application of metabolic engineering to threonine production, including the release of feedback regulation, elimination of metabolic bottlenecks, intensification of threonine export and inactivation of threonine catabolism, was stepwise resolved in E. coli by rounds of "design-build-test-learn" cycles through the iterative PS-Brick paradigm, and 45.71 g/L threonine was obtained through fed-batch fermentation. In addition to the value of the iterative character of PS-Brick for sequential strain engineering, seamless cloning enabled precise in-frame fusion for codon saturation mutagenesis and bicistronic design, and the repetitive sequence cloning ability of PS-Brick enabled construction of tandem CRISPR sgRNA arrays for genome editing. Moreover, the heterologous pathway deriving 1-propanol pathway from threonine, composed of Lactococcus lactis kivD and Saccharomyces cerevisiae ADH2, was assembled by one cycle of PS-Brick, resulting in 1.35 g/L 1-propanol in fed-batch fermentation. Conclusions: To the best of our knowledge, the PS-Brick framework is the first RE-assisted DNA assembly method using the strengths of both Type IIP and IIS REs. In this study, PS-Brick was demonstrated to be an efficient DNA assembly method for pathway construction and genome editing and was successfully applied in design-build-test-learn (DBTL) cycles of metabolic engineering for the production of threonine and threonine-derived 1-propanol. The PS-Brick presents a valuable addition to the current toolbox of synthetic biology and metabolic engineering.

16.
Stem Cells Dev ; 28(18): 1236-1252, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31311463

RESUMO

Restoring ß-cell mass by the transplantation of pancreatic islets is an effective diabetes treatment, but it is limited by the shortage of donor organs. CD133-expressing pancreatic ductal epithelial cells (PDECs) have the ability to generate insulin-producing cells. The expansion of these cells is dependent on extrinsic niche factors, but few of those signals have been identified. In this study, CD133-expressing PDECs were purified by sorting from adult wild-type C57BL/6 mice and TGFßRIInull/null mice. Furthermore, using immunofluorescence and transplantation assays, we found that the inhibition of the transforming growth factor-ß (TGF-ß) pathway promoted the expansion of CD133-expressing PDECs for many generations and maintained the ability of CD133-expressing PDECs to generate insulin-producing cells. Moreover, western blot, qRT-PCR, and dual luciferase assays using TGF-ß inhibitors were performed to identify the mechanisms by which TGF-ß signaling regulates proliferation and differentiation. The results showed that the inhibition of TGF-ß signaling enhanced Id2 binding to the promoter region of the cell proliferation repressor p16 and promoted the expansion of CD133-expressing PDECs, and the increased Id2 binding to NeuroD1 decreased the transcription of Pax6 to maintain CD133-expressing PDECs in the Pdx1-expression stage. Taken together, the effect of TGF-ß antagonists on CD133-expressing PDECs reveals a novel paradigm of signaling that explains the balance between the expansion and differentiation of pancreatic duct epithelial progenitors.

17.
Org Biomol Chem ; 17(29): 6975-6979, 2019 07 24.
Artigo em Inglês | MEDLINE | ID: mdl-31309215

RESUMO

Hydrazine is a substance harmful to humans and the environment, necessitating the development of a sensitive and specific detection method. Herein, a novel chemiluminescent probe based on Schaap's adamantylidene-dioxetane (CL-HZ) for detecting hydrazine was synthesized and evaluated. The probe was activated by the removal of a protecting group to form free phenoxy-dioxetane, when treated with hydrazine. It then showed a noticeable and sustainable fluorescent response without excitation, with intensity increasing 51-fold in the presence of hydrazine. CL-HZ can be used in 96-well high-throughput assays for analysis of hydrazine in polluted water samples and was successfully applied for the detection of hydrazine in living cells.


Assuntos
Adamantano/química , Corantes Fluorescentes/química , Compostos Heterocíclicos com 1 Anel/química , Hidrazinas/análise , Poluentes Químicos da Água/química , Células HeLa , Humanos , Medições Luminescentes , Estrutura Molecular , Imagem Óptica
18.
Clin Biomech (Bristol, Avon) ; 68: 73-79, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31158592

RESUMO

BACKGROUND: This study aimed to analyze the effects of intracapsular pressure (IAP) on blood flow in the femur after a femoral neck fracture. METHODS: Four simplified vascular models were used to measure the effect of vessel length on arterial blood flow in 10 New Zealand white rabbits. Ten models were evaluated under 10 different blood pressures. FINDINGS: IAP increased following fracture of the femoral neck, and deformation had the greatest potential effect on blood flow in the retinacular artery. When blood pressure was fixed at 60 mm Hg, an increase in IAP caused a reduction in blood flow. When the IAP was relatively high (above 60 mm Hg), and higher than the blood pressure, blood flow continued to drop as intracapsular pressure increased. Shortening of blood vessels had no significant effect on blood supply. However, the p-value was uniformly significant (<0.05) when stretched and twisted blood vessels were compared with normal blood vessels. INTERPRETATION: The results of computational fluid-structure interaction similarly indicated that a smaller blood vessel diameter and twisted blood vessels will result in decreased flow velocity when IAP increases. This study also revealed a close relationship between IAP and the hip joint's position and traction.

19.
R Soc Open Sci ; 6(4): 182040, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31183135

RESUMO

The ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate ([EMIm]Ac) was investigated as a promising absorbent for absorption refrigeration. To improve the thermal conductivity of pure [EMIm]Ac, IL-based nanofluids (ionanofluids, INFs) were prepared by adding graphene nanoplatelets (GNPs). The thermal stability of the IL and INFs was analysed. The variations of the thermal conductivity, viscosity and specific heat capacity resulting from the addition of the GNPs were then measured over a wide range of temperatures and mass fractions. The measured data were fitted with appropriate equations and compared with the corresponding classical models. The results revealed that the IL and INFs were thermally stable over the measurement range. The thermal conductivity greatly increased with increasing mass fraction, while only slightly changed with increasing temperature. A maximum enhancement in thermal conductivity of 43.2% was observed at a temperature of 373.15 K for the INF with a mass fraction of 5%. The numerical results revealed that the dispersion of the GNPs in the pure IL effectively improved the local heat transfer coefficient by up to 28.6%.

20.
Int J Mol Med ; 44(1): 67-78, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31115486

RESUMO

Lysophosphatidic acid (LPA), a simple water­soluble glycerophospholipid with growth factor­like activity, regulates certain behaviors of multiple cancer types by binding to its receptor, LPA receptor 2 (LPA2). Notch1 is a key mediator in multiple human cancer cell types. The association between LPA2 and Notch1 in gastric cancer cells is not well known. The present study aimed to investigate the function of LPA2 and Notch1 in controlling the migration and invasion activities of SGC­7901 gastric cancer cells following stimulation with LPA. It was revealed that LPA may stimulate the expression of Notch1 and Hes family bHLH transcription factor 1, and the phosphorylation of protein kinase B which belongs to the Notch pathway. Furthermore, by performing transwell migration and invasion assays, immunofluorescent staining, analyzing the expression of markers for the epithelial­mesenchymal transition (EMT) and downregulating LPA2 and Notch1 expression, it was verified that LPA2 and Notch1 mediated the metastasis, invasion, EMT and rebuilding of the cytoskeleton of SGC­7901 cells upon LPA treatment. An immunoprecipitation assay revealed that LPA2 interacted with Notch1 in SGC­7901 cells. The present study may provide novel ideas and an experimental basis for identifying the factors that affect the functions of SGC­7901 cells.


Assuntos
Movimento Celular/efeitos dos fármacos , Lisofosfolipídeos/farmacologia , Proteínas de Neoplasias/metabolismo , Receptor Notch1/metabolismo , Receptores de Ácidos Lisofosfatídicos/metabolismo , Transdução de Sinais/efeitos dos fármacos , Neoplasias Gástricas/metabolismo , Linhagem Celular Tumoral , Humanos , Invasividade Neoplásica , Neoplasias Gástricas/patologia
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