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1.
J Infect ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32027871

RESUMO

OBJECTIVES: this study aims to identify the potential marker for hepatic cystic echinococcosis (CE) activity. METHODS: totally 97 CE patients and 47 health control (HC) subjects were included. Based on the ultrasound (US) images, CE patients were further categorized as active CE (ACE; 52 cases) and inactive CE (IACE; 45 cases). The sST2 and IL (interleukin)-33 levels were measured by enzyme-linked immunosorbent assay. Univariate, multivariate and subgroup analysis were performed. The relationship between sST2 and CE activity was analyzed by the smooth curve fitting. The receiver operating characteristic (ROC) curve analysis was also performed. RESULTS: the serum sST2 level in the CE patients was significantly higher than the HC subjects. Moreover, there was significant difference in the serum sST2 level between the ACE and IACE group. However, no significant difference was observed in the IL-33 level between the ACE and IACE groups. Univariate analysis showed that CE activity was significantly correlated with the sST2 level when the sST2 was greater than 1496.6 pg/mL. Multivariable analysis after adjustment for potential confounding factors, and subgroup analysis showed that sST2 had independent effect on CE activity. ROC curve showed that sST2 could differentially diagnose CE activity at the cut-off value of 1246.550 pg/mL with an AUC of 0.770. CONCLUSION: the sST2 could be used as a biomarker for hepatic CE activity. High levels of sST2 suggest that the hepatic CE is more likely to be active. Our findings have important guiding significance for the diagnosis of CE activity and the choice of treatment methods.

2.
Int J Clin Oncol ; 25(1): 175-186, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31473884

RESUMO

PURPOSE: To determine whether patients can avoid systematic prostate biopsy (PBx) if their Prostate Imaging Reporting and Data System version 2 (PI-RADs v2) score is ≤ 3 and how we clinicians make decisions that can maximize benefit. MATERIALS AND METHODS: We reviewed our prospectively maintained database of consecutive men who received transrectal ultrasound-guided 24-core biopsy as well as pre-biopsy multi-parametric magnetic resonance imaging (mp-MRI). Of the 1276 men who were performed PBx in our institution from 2012 to July 2018, 491 patients conformed to the criteria. Negative predictive value (NPV) of negative mp-MRI (defined as PI-RADs < 3) combined prostate-specific antigen density (PSAD) were calculated. Models based on PI-RADs v2 were developed to predict the absence of clinically significant prostate cancer (CSPCa) and prostate cancer (PCa). Nomograms as well as receiver operating curves (ROC) were established to estimate the discrimination. Calibration curves were used to assess the concordance between predictive value and true risk. Decision curves were made to measure the overall net benefit. RESULTS: Prostate cancer and CSPCa detection rates were 21.6%, 7.3% and 36.7%, 23.4% in PIRADs v2 < 3 cohort and PIRADs v2 = 3 cohort, respectively. Men with biopsy-proved CSPCa had higher prostate-specific antigen (PSA), lower prostate volume (PV) and higher PSAD (all p < 0.05 in the two cohorts) than patients with clinically insignificant prostate cancer (CIPCa) or negative results. NPV of negative mp-MRI for detection of PCa was much higher when the PSAD was less than 0.15 (p < 0.001) and 0.2 for CSPCa (p = 0.007). According to multivariate analysis, we developed the model comprising Age, PSAD and PI-RADs v2 to predict the absence of CSPCa and PCa. The area under the curve (AUC) of the model for non-CSPCa was 0.75 (95% CI 0.68-0.80, PSAD cutoff 0.20), better than 0.71 (95% CI 0.65-0.80, PSAD cutoff 0.15). As for model for non-PCa, the AUC was 0.76 (95% CI 0.70-0.80, PSAD cutoff 0.15), higher than 0.71(95% CI 0.67-0.78, PSAD cutoff 0.20). Internally validated calibration curves showed that the model might overestimated the risk of the absence of CSPCa when the threshold was between 53 and 72%, and if the threshold was between 72 and 87%, it might underestimate the risk. As for the absence of PCa, the model might overestimate the risk between 52 and 76%. Decision curves showed that a better clinical net benefit was met when the threshold was 55% for non-PCa and 70% for non-CSPCa. CONCLUSIONS: NPV of negative mp-MRI for detection of CSPCa and PCa was improved with decreasing PSAD. The nomograms based on PI-RADs v2, age and PSAD showed internally validated high discrimination and calibration for the absence of PCa and CSPCa. When the predictive value was greater than 70% for the absence of CSPCa and 55% for the absence of PCa, we could avoid unnecessary PBx to maximize net benefit.

3.
Exp Ther Med ; 18(5): 3829-3836, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31656538

RESUMO

Research conducted previously has indicated that microRNAs (miRs) have potential effects on the pathogenesis of hepatocellular carcinoma (HCC). The biological functions of miR-552 have been well documented in colon cancer; however, the role of miR-552 in HCC remains unclear. The present study evaluated the effects of miR-552 in HCC physiology, using HCC cell lines as model. An miR-552 inhibitor was transfected into HCC cell lines to knock down the expression of miR-552. Reverse transcription-quantitative PCR and western blot analysis were used to detect the expression of miR-552 and Runt-related transcription factor 3 (RUNX3), respectively. MTT assay was used to analyze cell viability, whilst Transwell and wound-healing assay were used to investigate cell migration. Flow cytometry was performed to measure cell apoptosis. The direct association between RUNX3 and miR-552 was evaluated using dual luciferase reporter assay. The expression of miR-552 was significantly elevated in HCC tumor tissues compared with the adjacent healthy samples. Additionally, transfection with the miR-552 inhibitor decreased cell viability and migration. miR-552 knockdown also increased HCC cell apoptosis in vitro. In conclusion, these results suggest that miR-552 has an oncogenic function in HCC and is a potential biomarker for detecting HCC.

4.
Exp Ther Med ; 18(4): 3109-3116, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31572551

RESUMO

The aim of the present study was to investigate the roles of type 2 innate lymphoid cells (ILC2s) and interleukin-33 (IL-33) in chronic obstructive pulmonary disease (COPD). Serum and peripheral blood mononuclear cells (PBMCs) were isolated from healthy controls and COPD patients. ILC2 cells from the peripheral blood of COPD patients were stimulated with IL-33 or neutralizing ST2 antibody+IL-33 in vitro. The cell viability was assessed using a Cell Counting Kit-8 assay. ELISA was used to detect serum IL-33 and the levels of IL-4, IL-5, IL-6, IL-13 and soluble ST2 (sST2) in the culture supernatant. The percentage of ILC2 cells was measured by flow cytometry. The mRNA expression levels of GATA binding protein 3 (GATA3), RAR-related orphan receptor (ROR)α, ST2 and prostaglandin D2 receptor 2 (CRTH2) were detected by reverse transcription-quantitative PCR. It was revealed that IL-33, IL-5, IL-6 and IL-13 were significantly elevated in peripheral blood of patients with COPD. The proportion of ILC2s in peripheral blood of COPD patients was significantly increased, and the expression of RORA and CRTH2 was increased. The proportion of ST2+ ILC2 cells was significantly increased. After 48 h of IL-33 stimulation in vitro, the ratio of linage-CD45+CD127+CRTH2+ cells reached a maximum. In addition, the viability of ILC2 cells, the expression levels of RORA, GATA3, ST2 and CRTH2 mRNA and the cytokines IL-4, IL-6, IL-5, IL-13 and sST2 were significantly increased. These effects were abrogated by treatment with anti-ST2. In conclusion, IL-33 is upregulated in the serum of patients with COPD and the proportion of ILC2s among the PBMCs is increased. IL-33 may promote the proliferation of ILC2 cells and secrete type 2 T-helper cell cytokines to participate in the immune response in COPD.

5.
Mol Immunol ; 114: 651-660, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31557626

RESUMO

Brucella poses a serious threat to human health. High quality vaccines for Brucella are urgently needed to effectively reduce the incidence of brucellosis. OMP2b and BCSP31 are important component proteins of the Brucella outer membrane and are highly immunogenic. Here, we used the bioinformatics software ProtParam, SOPMA, SWISS-MODEL, Rasmol, BepiPred, SYFPEITHI and IEDB to analyze the structure of these two proteins and predict the epitopes of T cells and B cells. Through analysis, we predicted three Th cell epitopes, seven CTL epitopes, eight B cell epitopes, and one T-B combined epitope of OMP2b protein. Subsequently, we also obtained three Th cell epitopes, six CTL epitopes, nine B cell epitopes and one T-B combined epitope of BCSP31 protein. The T-B combined epitopes and CTL epitopes of OMP2b and those of BCSP31 were synthesized to detect their immunogenicity. The IFN-γ ELISPOT assay showed that the T-B combined epitope peptides of OMP2b and BCSP31 activated Th cell immune responses. ELISA analysis detected the specific antibodies against the T-B combined epitope peptide of OMP2b and BCSP31 in the serum of Brucellosis patients. Additionally, CTL epitope peptide of OMP2b and BCSP31 proteins promoted the secretion of soluble perforin and granzyme B in the culture supernatant. In conclusion, our study shows that the T-B combined epitopes and CTL epitopes of OMP2b and BCSP31 have immunogenicity and immunoreactivity. Our results may lay a theoretical foundation for the development of vaccines against Brucella.


Assuntos
Proteínas de Bactérias/imunologia , Brucella/imunologia , Brucelose/imunologia , Epitopos/imunologia , Porinas/imunologia , Vacinas/imunologia , Adolescente , Adulto , Antígenos de Bactérias/imunologia , Biologia Computacional/métodos , ELISPOT/métodos , Epitopos de Linfócito T/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
6.
Mediators Inflamm ; 2019: 6519427, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31316301

RESUMO

This study is to investigate the role of regulatory B (Breg) cells in cervical cancer. In total, 70 cases of cervical cancer, 52 cases of cervical intraepithelial neoplasia (CIN), and 40 normal controls were enrolled. The percentage of Breg cells was detected by flow cytometry. Serum levels of IL-10 were measured by ELISA. The correlation between Breg cells and the clinical characterizations of cervical cancer was analyzed. The inhibition effect of Breg cells on CD8+ T cells was tested by blocking IL-10 in vitro. The percentage of CD19+CD5+CD1d+ Breg cells and the level of IL-10 of patients with cervical cancer or CIN were significantly higher than those in the control group (P < 0.05). And the postoperative levels of Breg cells and IL-10 were significantly lower than the preoperative levels (P < 0.05). Breg cells and the IL-10 level were positively correlated in cervical cancer patients (r = 0.516). In addition, the Breg cell percentage was closely related to the FIGO stages, lymph node metastasis, tumor differentiation, HPV infection, and the tumor metastasis of cervical cancer (P < 0.05). The Breg cell percentage was negatively correlated with CD8+ T cells of cervical cancer patients (r = -0.669). The level of IL-10 in the culture supernatant of Bregs treated with CpG was significantly higher than that of non-Bregs (P < 0.05). After coculture with Bregs, the quantity of CD8+ T cells to secrete perforin and Granzyme B was significantly decreased, and this effect was reversed after blocking IL-10 by a specific antibody. Breg cells are elevated in cervical cancer and associated with disease progression and metastasis. Moreover, they can inhibit the cytotoxicity of CD8+ T cells.

7.
Mediators Inflamm ; 2019: 3140183, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31320835

RESUMO

To investigate the effect of ILC2s on Th2-type adaptive immunity during the acute exacerbation of chronic obstructive pulmonary disease (AECOPD), the study enrolled healthy people, stable COPD patients, and AECOPD patients. Flow cytometry was used to detect Th1, Th2, and ILC2 in the peripheral blood and CD80 and MHC II levels on ILC2. The mRNA levels of GATA3, RORα, and CRTH2 of ILC2s were detected by RT-PCR. In addition, ILC2s from the peripheral blood of AECOPD patients were cocultured with CD4+ T cells from the peripheral blood of healthy controls. Cytokine levels in serum of the three groups and the in vitro coculture supernatants were measured by ELISA. Compared with the stable COPD group or the healthy control group, Th2 in the peripheral blood of AECOPD group increased dramatically, inducing an increase of Th2/Th1 ratio in AECOPD patients. Meanwhile, the level of IL-4 in the serum of this group was also increased. However, we also detected ILC2s in the peripheral blood of the AECOPD group and found that it was also increased, alone with the increased GATA3, RORα, and CRTH2 mRNA levels. We also found that the CD80 and MHC II on ILC2 were significantly upregulated and the proportion of MHC II+ ILC2 cells was significantly positively correlated with the proportion of Th2 cells in AECOPD patients. To further demonstrate the effect of ILC2 on Th2 cells, we cocultured ILC2 with CD4+ T cells in vitro, which also showed a significant increase of Th2 ratio as well as Th2-associated cytokines IL-4, IL-5, and IL-13. However, we found that this effect of ILC2s on Th2 cells could be inhibited by the addition of anti-MHC II. The Th2/Th1 balance shifts to Th2 in AECOPD. ILC2s may function as APC by the upregulation of MHC II and regulate adaptive immunity shift to Th2-type response in AECOPD.

8.
Infect Genet Evol ; 73: 98-108, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31022474

RESUMO

Cystic echinococcosis (CE) is a global zoonosis caused by the larvae of the parasite Echinococcus granulosus (E. granulosus). According to its life cycle and previous studies of antigen candidates for vaccines against E. granulosus, we chose two proteins expressed at different stages of the E. granulosus life cycle to design a multi-epitope vaccine. The EgA31 antigen gene is derived from the adult stage of E. granulosus, and the EgG1Y162 antigen gene is derived from the larval stage of E. granulosus. In this study, we used several bioinformatics methods to analyze various aspects of the EgA31 and EgG1Y162 proteins, including the physicochemical properties, secondary and tertiary structures, and the dominant T-cell and B-cell epitopes. The results showed that EgA31 protein was an unstable and hydrophilic protein, while EgG1Y162 was stable and hydrophobic. The secondary structure of the EgA31 protein consisted of 82.36% alpha helixes, 4.16% extended strands, 3.16% beta turns and 10.32% random coils. The secondary structure of EgG1Y162 consisted of 33.33% alpha helixes, 25.49% extended strands, 5.88% beta turns and 35.29% random coils. Moreover, our results identified 6 dominant T-cell epitopes and 5 dominant B-cell epitopes in the EgA31 protein structure and 6 dominant T-cell epitopes and 3 dominant B-cell epitopes in EgG1Y162. In conclusion, this study provides comprehensive biological information about the EgA31 and EgG1Y162 proteins, which will lay a theoretical foundation for multi-epitope vaccines against Echinococcus granulosus.

9.
J Cancer Res Ther ; 15(2): 380-385, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30964115

RESUMO

Background: The value of saturation prostate biopsy (SPBx) in patients without biopsy history remains controversial. Materials and Methods: A total of 644 consecutive suspected prostate cancer (PCa) cases without biopsy history were retrospectively grouped as 24-core transperineal SPBx (n = 368) guided by coaxial needle and prostate transperineal biopsy (PBx) (systematic 14-core scheme, n = 276). PCa detection rates were compared based on prostate-specific antigen (PSA) levels, PSA density (PSAD), and Gleason scores. Complications associated with those procedures were assessed. Results: Coaxial SPBx was superior to the 14-core scheme for overall PCa detection rates (39.67% vs. 24.64%), at PSA levels of 4.1-10.0 ng/ml (37.37% vs. 23.48%) or 10.1-20.0 ng/ml (43.31% vs. 27.21%), and PSAD <0.15 (47.80% vs. 29.23%) or 0.15-0.24 (34.07% vs. 20.93%) (all P < 0.05). In patients with positive biopsy, the rates of nonclinically significant PCa were comparable between the two biopsy groups. Conclusions: Transperineal coaxial needle SPBx as the initial scheme improves PCa detection compared with initial systematic 14-core PBx, without increasing complications and overdiagnosis.


Assuntos
Biópsia com Agulha de Grande Calibre , Neoplasias da Próstata/diagnóstico , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais , Biópsia com Agulha de Grande Calibre/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Estadiamento de Neoplasias , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/terapia , Carga Tumoral
10.
Sci Rep ; 9(1): 3512, 2019 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-30837598

RESUMO

Given brucellosis is a widespread zoonosis in the world, a safe and effective vaccine is urgently needed. Recent trend in vaccine design has shifted to epitope-based vaccines that are safe and specific. In this study, peptide containing both T-cell and B-cell epitopes of OMP31 was synthesized and used to immunize the mice by nasal administration. The protective efficacy was evaluated. Mice immunized with the B epitope or TB epitope peptides of OMP31 had higher levels of IgG1 and IgG2a in the serum. While the BALB/c mice immunized with peptides containing T cell epitope or TB epitope of OMP31 showed high degree of IFN-γ-producing T cells in the lymphocytes from the respiratory draining lymph nodes and spleen. After intranasally challenged with 5 × 105 CFU of Brucella melitensis (strain 16 M), the bacterial loads in lung of the immunized mice were significantly lower than control group. These data demonstrate for the first time that peptides of OMP31 containing T epitope, B epitope or TB epitopes are of high immunogenicity and thus can protect host from Brucella melitensis infection in lung.

11.
Proc Natl Acad Sci U S A ; 115(20): E4584-E4593, 2018 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-29712835

RESUMO

Formation of the androgen receptor splicing variant 7 (AR-V7) is one of the major mechanisms by which resistance of prostate cancer to androgen deprivation therapy occurs. The histone demethylase JMJD1A (Jumonji domain containing 1A) functions as a key coactivator for AR by epigenetic regulation of H3K9 methylation marks. Here, we describe a role for JMJD1A in AR-V7 expression. While JMJD1A knockdown had no effect on full-length AR (AR-FL), it reduced AR-V7 levels in prostate cancer cells. Reexpression of AR-V7 in the JMJD1A-knockdown cells elevated expression of select AR targets and partially rescued prostate cancer cell growth in vitro and in vivo. The AR-V7 protein level correlated positively with JMJD1A in a subset of human prostate cancer specimens. Mechanistically, we found that JMJD1A promoted alternative splicing of AR-V7 through heterogeneous nuclear ribonucleoprotein F (HNRNPF), a splicing factor known to regulate exon inclusion. Knockdown of JMJD1A or HNRNPF inhibited splicing of AR-V7, but not AR-FL, in a minigene reporter assay. JMJD1A was found to interact with and promote the recruitment of HNRNPF to a cryptic exon 3b on AR pre-mRNA for the generation of AR-V7. Taken together, the role of JMJD1A in AR-FL coactivation and AR-V7 alternative splicing highlights JMJD1A as a potentially promising target for prostate cancer therapy.


Assuntos
Processamento Alternativo , Regulação Neoplásica da Expressão Gênica , Ribonucleoproteínas Nucleares Heterogêneas Grupo F-H/metabolismo , Histona Desmetilases com o Domínio Jumonji/metabolismo , Neoplasias da Próstata/genética , Receptores Androgênicos/genética , Animais , Proliferação de Células , Epigênese Genética , Éxons , Ribonucleoproteínas Nucleares Heterogêneas Grupo F-H/genética , Histonas/genética , Histonas/metabolismo , Humanos , Histona Desmetilases com o Domínio Jumonji/genética , Masculino , Camundongos Endogâmicos NOD , Camundongos SCID , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , RNA Mensageiro , Células Tumorais Cultivadas , Ensaios Antitumorais Modelo de Xenoenxerto
12.
J Infect ; 76(4): 406-416, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29391143

RESUMO

OBJECTIVES: Cystic echinococcosis (CE) is a zoonotic disease caused by Echinococcus granulosus (Eg) infection. Th9 cells are reported to be involved in the immune responses in CE patients. This study aims to investigate the role of TGF-ß/Smad pathway in the regulation of Th9 cells in CE patients. METHODS: Using Western blot analysis, flow cytometry, qPCR, immunohistochemistry, ELISA and MTT assay, we measured the expression levels of TGF-ß/Smad, PU.1, IRF-4, and IL-9 in CE patients. RESULTS: The levels of TGF-ß, p-Samd3, PU.1 and IL-9 were elevated in the liver of CE patients. IL-9 and IL-9R expressions were also elevated in the infected liver tissue, and IL-9 level was positively correlated with the liver inflammation. The levels of IL-9, IL-4, TGF-ß and IL-10 in the supernatant were also significantly increased after stimulating hepatic lymphocytes of CE patients with Eg antigen B. After blocking the TGF-ß pathway signaling in vitro, PU.1 and IL-9 were obviously reduced. CONCLUSIONS: IL-9 may aggravate the inflammatory response in the liver of CE patients. The TGF-ß/Smad signaling pathway is activated, and the signaling pathway may promote the differentiation of Th9 cells and IL-9 expression in active CE patients.


Assuntos
Linfócitos T CD4-Positivos/citologia , Equinococose/imunologia , Interleucina-9/imunologia , Transdução de Sinais , Proteínas Smad/imunologia , Fator de Crescimento Transformador beta/imunologia , Adulto , Animais , Antígenos de Helmintos/farmacologia , Linfócitos T CD4-Positivos/imunologia , Diferenciação Celular , Citocinas/imunologia , Echinococcus granulosus , Hepatócitos/efeitos dos fármacos , Hepatócitos/imunologia , Humanos , Fígado/imunologia , Fígado/parasitologia , Masculino , Pessoa de Meia-Idade , Proteínas Proto-Oncogênicas/imunologia , Transativadores/imunologia , Regulação para Cima
13.
Mol Immunol ; 94: 183-189, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29328998

RESUMO

AIMS: This study is to predict and purify the T-B combined epitopes of egG1Y162 antigen in Echinococcus granulosus, and to evaluate their immunogenicity in mice. METHODS: The bioinformatics software was used to predict the T-B combined epitopes of egG1Y162 antigen. Recombinant egG1Y161/2 peptides were constructed, expressed and purified. Mice were immunized with egG1Y161/2 peptides. The serum and spleen cells were isolated. The isolated spleen cells were stimulated with egG1Y161/2 peptides in vitro and the culture supernatant was collected. The levels of IgG in serum and levels of IL-4 and IFN-γ in the culture supernatant were measured by ELISA. The weight and number of the fresh hydatid cysts were evaluated. The serum ptotoscolicidal activity was measured by the complement dependent cytotoxicity assay. RESULTS: Peptides of 6-19aa, 64-82aa, 106-119aa were predicted as T-B combined epitopes of egG1Y162 antigen. And, recombinant protein egG1Y162-1 or egG1Y162-2, which contained T-B combined epitope(s) of the 6-19aa, or the 64-82aa and the 106-119aa in egG1Y162 antigen, respectively, was successfully expressed and purified. Serum IgG levels of mice immunized with egG1Y162-1/2 were significantly increased during the immune response to Echinococcus granulosus. The levels of IFN-γ, IL-4 and the ratio of IFN-γ/IL-4 after egG1Y162-1/2 immunization were significantly higher. Weight and number of the fresh hydatid cysts in egG1Y162-1/2 immunized mice was significantly decreased. And, the serum protoscolicidal activity after egG1Y162-1/2 immunization was enhanced. CONCLUSIONS: The egG1Y162-1/2 induces production of serum IgG levels and Th1 cell immune response, which enhances the protective immunity in Echinococcus granulosus challenged mice and thus may be used as a potential vaccine candidate.


Assuntos
Antígenos de Helmintos/uso terapêutico , Equinococose/prevenção & controle , Echinococcus granulosus/imunologia , Imunização/métodos , Sequência de Aminoácidos , Animais , Antígenos de Helmintos/química , Antígenos de Helmintos/imunologia , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Proteínas Recombinantes/química , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/uso terapêutico
14.
Zhongguo Zhong Yao Za Zhi ; 42(4): 663-668, 2017 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-28959834

RESUMO

Collecting different commodity grade Gardenia jasminoides of wild and cultivated varieties all over the country, obtaining color information from each batch of G. jasminoides by the standard D65 light source and image acquisition system, quantifing the gardenia plumpness information by the digital display vernier caliper, determinating 6 kinds of effective components of G. jasminoides by HPLC, classifing from ten indicators by two step clustering analysis and correspondence analysis method of statistics, clearing the importance of the traditional identification indexes, establishing multiple corresponding relation between the skin color and commercial specification of G. jasminoides,exploring the correlation of the skin color and chemical composition, to provide the reference for the reasonable division of commercial specifications and grades of G. jasminoides. Medicine is divided into two classes and has obvious distinguish meaning, The importance of the skin color is greater than the plumpness in traditional identification characteristics, it can accurately distinguish the specifications of G. jasminoides. We improve and rebuild the standard of commodity specifications and grades of Gardenia jasminoides Ellis and establish the rapid evaluation method by the study, it provide a new way and idea for the comprehensive evaluation of G. jasminoides quality.


Assuntos
Gardenia/química , Controle de Qualidade , Cromatografia Líquida de Alta Pressão , Plantas Medicinais/química
15.
PLoS Negl Trop Dis ; 11(5): e0005577, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28459811

RESUMO

BACKGROUND: This study aims to analyze the clinical characteristics and treatment outcomes of 590 patients with brucellosis in Xinjiang, China. METHODOLOGY AND PRINCIPAL FINDINGS: The clinical characteristics, laboratory findings, complications and prognosis of 590 patients infected with brucellosis were retrospectively analyzed. These patients had a mean age of 44.24 ± 15.83 years with 60.5% having a history of close contacting with cattle and sheep. Of them, 53.6% (316 /590) were in acute phase and 21.5% were in chronic phase. Agglutination test showed 98.5% positive with 34% blood culture positive of Brucella. The major symptoms were fatigue (91%), hyperhidrosis(88.1%), fever(86.9%), and joint pain(81%) with 29.8% having enlarged liver, 26.1% having enlarged spleen and 23.2% having osteoarticular complications. Combination of doxycycline plus rifampicin for 12 weeks was an effective regimen for patients without complications. The 3-drug regimen (doxycycline+rifampicin+levofloxacin) for 12 weeks was recommended for these with complications. There were 6 patients died (1.02%) with overall relapse rate of 5.98%. CONCLUSIONS: Brucellosis is mostly associated with contacting with domestic animal production in Xinjiang, China. Clinical symptoms include fever, fatigue, hyperhidrosis, and joint pain with common complication of osteoarticular involvement. Three-drug-regimen of doxycycline+rifampicin+levofloxacin for 12 weeks was effective for these patients with complications.


Assuntos
Brucella/isolamento & purificação , Brucelose/complicações , Brucelose/tratamento farmacológico , Adolescente , Adulto , Idoso , Animais , Animais Domésticos , Antibacterianos/administração & dosagem , Artralgia/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Doxiciclina/administração & dosagem , Quimioterapia Combinada , Feminino , Febre/epidemiologia , Hepatomegalia/epidemiologia , Humanos , Levofloxacino/administração & dosagem , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Rifampina/administração & dosagem , Esplenomegalia/epidemiologia , Resultado do Tratamento , Adulto Jovem
17.
Zhongguo Zhong Yao Za Zhi ; 42(23): 4636-4640, 2017 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-29376264

RESUMO

Seven compounds(deacetylasperulasidic acid methyl ester, gardenoside, chlorogenic acid, geniposide, crocin-Ⅰ, crocin-Ⅱ, chikusetsu saponin Ⅳa)were determined simultaneously by multiple wavelength HPLC with diode array detector(DAD) in different parts of Gardenia jasminoides. The results showed that these components in different parts of G. jasminoides had a different distribution, and there was a large difference in content of each component. Geniposide was mainly distributed in fruits and leaves; chikusetsu saponin Ⅳa was mainly distributed in roots and stems; crocus glycosides existed mainly in fruits; chlorogenic acid had a higher distribution in leaves and stems; gardenoside had a higher distribution in leaves and roots, while ceacetylasperulasidic acid methyl ester had a higher distribution in roots and stems. Based on the analysis of the chemical composition and content difference in different parts of G. jasminoides, the basis for the comprehensive utilization and quality evaluation of resources of G. jasminoides was provided.


Assuntos
Frutas/química , Gardenia/química , Compostos Fitoquímicos/análise , Folhas de Planta/química , Ácido Clorogênico/análise , Cromatografia Líquida de Alta Pressão , Iridoides/análise
18.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 32(12): 1675-1678, 2016 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-27916101

RESUMO

Objective To investigate the alterations of soluble molecule programmed death 1 (sPD-1) and soluble programmed death ligand 1 (sPD-L1), as well as related Th1 cytokines IFN-γ and Th2 cytokines IL-4 in patients infected with Brucella. Methods The study enrolled 56 patients infected with Brucella and 48 healthy controls. Serum sPD-1 and sPD-L1 were detected by ELISA and the levels of Th1/Th2 cytokines were measured with cytometric bead array (CBA). Among them, 37 patients received follow-up. Results Compared with the control group, the serum levels of sPD-1/sPD-L1 and IL-4 increased significantly, while the level of IFN-γ decreased significantly. In the 37 patients receiving follow-up after treatment, serum levels of sPD-1/sPD-L1 and IL-4 were remarkably lower than those in pretherapy, while the level of IFN-γ was obviously higher than that in pretherapy. Conclusion The increase of sPD-1 and sPD-L1 may be involved in the process of Brucella infection.


Assuntos
Antígeno B7-H1/sangue , Brucella/patogenicidade , Brucelose/sangue , Receptor de Morte Celular Programada 1/sangue , Adulto , Citocinas/sangue , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Interferon gama , Interleucina-4/sangue , Masculino , Células Th1/metabolismo , Células Th2/metabolismo , Adulto Jovem
19.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 32(7): 968-71, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-27363280

RESUMO

Objective To detect the serum levels of soluble T cell immunoglobulin mucin molecule 3 (sTim-3), IFN-γ and IL-4 in patients with pulmonary tuberculosis. Methods Peripheral blood was collected from 48 patients with pulmonary tuberculosis and 20 healthy controls. The serum level of sTim-3 was detected by ELISA. The levels of IFN-γ and IL-4 were determined by cytokine bead array (CBA). The correlations between sTim-3, IFN-γ and IL-4 levels in sera of patients with pulmonary tuberculosis were analyzed by Pearson correlation test. Results Compared with the control group, the serum levels of sTim-3 and IL-4 increased significantly, while the level of IFN-γ decreased significantly. Pearson analysis showed that there was a negative correlation between sTim-3 and IFN-γ as well as between IFN-γ and IL-4 in patients with pulmonary tuberculosis, while sTim-3 and IL-4 were positively correlated. Conclusion The serum levels of sTim-3 and IL-4 increase, but IFN-γ decreases in the patients with pulmonary tuberculosis.


Assuntos
Receptor Celular 2 do Vírus da Hepatite A/sangue , Interferon gama/sangue , Interleucina-4/sangue , Tuberculose Pulmonar/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Solubilidade , Adulto Jovem
20.
Zhong Yao Cai ; 39(1): 37-41, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30079699

RESUMO

Objective: To clone the acetyl-CoA C-acetyl transferase( AACT) gene from Isodon rubescens, and to analyze the bioinformatics and expression of the gene. Methods: According to the IrAACT gene sequence of Isodon rubescens transcriptome,a pair of primers was designed, and the ORF of cDNA sequence was obtained by reverse transcription PCR. Bioinformatic analysis of this gene and its corresponding protein were performed. Real-time quantitative PCR( q PCR) was used to detect the relative expression levels of IrAACT different tissues of Isodon rubescens. Results: The IrAACT cDNA sequence contained a 1 254 bp open reading frame and encoded a predicted protein of 417 amino acids. IrAACT had extensive homology with AACTs from other plant species, such as Salvia miltiorrhiza, et al. Bioinformatic analysis showed that IrAACT-encoding protein contained the thiolase Ⅱ catalytic domain. q PCR analysis showed that the expression of IrAACT was tissue-specific, and accumulation of transcripts was greater in flowers and leaves, followed by stems, roots and callus. Conclusion: It is the first time to report IrAACT gene and its relative expression level. The results will provide a groundwork for studying the function of IrAACT in terpenoid biosynthesis of Isodon rubescens.


Assuntos
Acetil-CoA C-Acetiltransferase/metabolismo , Sequência de Aminoácidos , Clonagem Molecular , DNA Complementar , Regulação da Expressão Gênica de Plantas , Isodon , Fases de Leitura Aberta , Filogenia , Folhas de Planta , Raízes de Plantas , Salvia miltiorrhiza
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