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1.
Int J Mol Sci ; 22(4)2021 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-33562315

RESUMO

Plasma membrane intrinsic proteins (PIPs) transport water, CO2 and small neutral solutes across the plasma membranes. In this study, we used the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 system (CRISPR/Cas9) to mutate PIP1;4 and PIP1;5 in a pip1;1,2,3 triple mutant to generate a pip1;1,2,3,4,5 (pip1s-) quintuple mutant. Compared to the wild-type (WT) plant, the pip1s- mutants had smaller sized rosette leaves and flowers, less rosette leaf number, more undeveloped siliques, shorter silique and less seeds. The pollen germination rate of the pip1s- mutant was significantly lower than that of the WT and the outer wall of the pip1s- mutant's pollen was deformed. The transcriptomic analysis showed significant alterations in the expression of many key genes and transcription factors (TFs) in the pip1s- mutant which involved in the development of leaf, flower and pollen, suggesting that the mutant of PIP1s not only directly affects hydraulics and carbon fixation, but also regulates the expression of related genes to affect plant growth and development.

2.
J Plant Physiol ; 257: 153328, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33373828

RESUMO

Anthocyanidins are important pigments that cause plant tissues to develop colors. They have attracted much attention due to their crucial regulatory roles in plant growth as well as their health benefits. In order to reveal the molecular mechanism of anthocyanidin synthesis and regulation in purple corn (Zea mays L.) in this study, purple corn 963 was used to compare differences in gene expression during three stages of grain development by transcriptome analysis. A total of 17,168 differentially expressed genes (DEGs) (7564 up-regulated and 9604 down-regulated DGEs) were identified. The DEGs were significantly enriched in "Phenylpropanoid biosynthesis", "Biosynthesis of secondary metabolites", and "Plant hormone signal transduction". In addition, 72 % of the structural genes that regulate anthocyanidin synthesis were up-regulated, and the transcription factors related to the accumulation of anthocyanidins were enriched during grain development. Moreover, the differential expression of phytohormone genes might also be an important factor in anthocyanidin accumulation. Transcriptomic analysis presents a molecular basis for the study of grain color changes in the three stages of grain development, and provides information for further research on the mechanism of anthocyanidin synthesis.

3.
Front Plant Sci ; 11: 574316, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32983222

RESUMO

Arabidopsis thaliana ENO2 (AtENO2) encodes two proteins AtENO2 (enolase) and AtMBP-1 (c-Myc binding protein 1-like). The loss of AtENO2 function causes the constitutive developmental defects which are correlated with reduced enolase activity, but not AtMBP-1 transcript abundance. However, the regulation mechanism of AtENO2 on the seed properties is still not clear. In this study, we found that the mutation of AtENO2 reduced the seed size and weight. The level of glucose in seed was significantly elevated but that of starch was decreased in AtENO2 mutants compared to WT plants. We also found that AtENO2 mutation reduced the content of cytokinin which resulted in smaller cotyledons. The RNA-seq data showed that there were 1892 differentially expressed genes and secondary metabolic pathways were significantly enriched. Instead of AtMBP-1, AtENO2 protein interacted with AtbZIP75 which may mediate the secondary metabolism. Therefore, ENO2 alters the size and weight of seeds which is not only regulated by the content of cytokinin and secondary metabolism, but may be affected by the interaction of ENO2 and bZIP57. These results are helpful to understand the novel function of AtENO2 which provide a foundation for further exploration of the key candidate genes for crop breeding.

4.
Physiol Plant ; 168(3): 660-674, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31343741

RESUMO

Arabidopsis thaliana ENO2 (AtENO2) plays an important role in plant growth and development. It encodes two proteins, a full-length AtENO2 and a truncated version, AtMBP-1, alternatively translated from the second start codon of the mRNA. The AtENO2 mutant (eno2- ) exhibited reduced leaf size, shortened siliques, a dwarf phenotype and higher sensitivity to abiotic stress. The objectives of this study were to analyze the regulatory network of the ENO2 gene in plant growth development and understand the function of AtENO2/AtMBP-1 to abiotic stresses. An eno2- /35S:AtENO2-GFP line and an eno2- /35S:AtMBP-1-GFP line of Arabidopsis were obtained. Results of sequencing by 454 GS FLX identified 578 upregulated and 720 downregulated differential expressed genes (DEGs) in a pairwise comparison (WT-VS-eno2- ). All the high-quality reads were annotated using the Gene Ontology (GO) terms. The DEGs with KEGG pathway annotations occurred in 110 pathways. The metabolic pathways and biosynthesis of secondary metabolites contained more DEGs. Moreover, the eno2- /35S:AtENO2-GFP line returned to the wild-type (WT) phenotype and was tolerant to drought and salt stresses. However, the eno2- /35S:AtMBP-1-GFP line was not able to recover the WT phenotype but it has a higher tolerance to drought and salt stresses. Results from this study demonstrate that AtENO2 is critical for the growth and development, and the AtMBP-1 coded by AtENO2 is important in tolerance of Arabidopsis to abiotic stresses.


Assuntos
Proteínas de Arabidopsis/fisiologia , Arabidopsis/fisiologia , Secas , Estresse Salino , Proteínas de Transporte , Regulação da Expressão Gênica de Plantas , Plantas Geneticamente Modificadas
5.
Genes (Basel) ; 10(6)2019 06 21.
Artigo em Inglês | MEDLINE | ID: mdl-31234426

RESUMO

Chitinase is a kind of hydrolase with chitin as a substrate and is proposed to play an essential role in plant defense system by functioning against fungal pathogens through degrading chitin. Recent studies indicated chitinase is also involved in abiotic stress response in plants, helping plants to survive in stressful environments. A. nanus, a rare evergreen broad-leaved shrub distrusted in deserts in Central Asia, exhibits a high level of tolerance to drought and low temperature stresses. To identify the chitinase gene involved in drought and low temperature responses in A. nanus, we performed genome-wide identification, classification, sequence alignment, and spatio-temporal gene expression analysis of the chitinases in A. nanus under osmotic and low temperature stress. A total of 32 chitinase genes belonging to glycosyl hydrolase 18 (GH18) and GH19 families were identified from A. nanus. Class III chitinases appear to be amplified quantitatively in A. nanus, and their genes carry less introns, indicating their involvement in stress response in A. nanus. The expression level of the majority of chitinases varied in leaves, stems, and roots, and regulated under environmental stress. Some chitinases, such as EVM0022783, EVM0020238, and EVM0003645, are strongly induced by low temperature and osmotic stress, and the MYC/ICE1 (inducer of CBF expression 1) binding sites in promoter regions may mediate the induction of these chitinases under stress. These chitinases might play key roles in the tolerance to these abiotic stress in A. nanus and have potential for biotechnological applications. This study provided important data for understanding the biological functions of chitinases in A. nanus.


Assuntos
Quitinases/genética , Resposta ao Choque Frio/genética , Fabaceae/genética , Filogenia , Quitinases/classificação , Secas , Fabaceae/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas/genética , Genoma de Planta/genética , Pressão Osmótica/fisiologia , Folhas de Planta/genética , Alinhamento de Sequência , Estresse Fisiológico/genética
6.
Int J Mol Sci ; 20(2)2019 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-30634475

RESUMO

Jojoba (Simmondsia chinensis) is a semi-arid, oil-producing industrial crop that have been widely cultivated in tropical arid region. Low temperature is one of the major environmental stress that impair jojoba's growth, development and yield and limit introduction of jojoba in the vast temperate arid areas. To get insight into the molecular mechanisms of the cold stress response of jojoba, a combined physiological and quantitative proteomic analysis was conducted. Under cold stress, the photosynthesis was repressed, the level of malondialdehyde (MDA), relative electrolyte leakage (REL), soluble sugars, superoxide dismutase (SOD) and phenylalanine ammonia-lyase (PAL) were increased in jojoba leaves. Of the 2821 proteins whose abundance were determined, a total of 109 differentially accumulated proteins (DAPs) were found and quantitative real time PCR (qRT-PCR) analysis of the coding genes for 7 randomly selected DAPs were performed for validation. The identified DAPs were involved in various physiological processes. Functional classification analysis revealed that photosynthesis, adjustment of cytoskeleton and cell wall, lipid metabolism and transport, reactive oxygen species (ROS) scavenging and carbohydrate metabolism were closely associated with the cold stress response. Some cold-induced proteins, such as cold-regulated 47 (COR47), staurosporin and temperature sensitive 3-like a (STT3a), phytyl ester synthase 1 (PES1) and copper/zinc superoxide dismutase 1, might play important roles in cold acclimation in jojoba seedlings. Our work provided important data to understand the plant response to the cold stress in tropical woody crops.


Assuntos
Resposta ao Choque Frio , Proteoma , Proteômica , Traqueófitas/metabolismo , Biologia Computacional/métodos , Regulação da Expressão Gênica de Plantas , Redes Reguladoras de Genes , Anotação de Sequência Molecular , Folhas de Planta/metabolismo , Mapeamento de Interação de Proteínas , Mapas de Interação de Proteínas , Plântula/metabolismo , Estresse Fisiológico , Traqueófitas/genética , Madeira
7.
Int J Mol Sci ; 20(1)2019 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-30609831

RESUMO

Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporin proteins located on plasma membranes where they facilitate the transport of water and small uncharged solutes. PIPs play an important role throughout plant development, and in response to abiotic stresses. Jojoba (Simmondsia chinensis (Link) Schneider), as a typical desert plant, tolerates drought, salinity and nutrient-poor soils. In this study, a PIP1 gene (ScPIP1) was cloned from jojoba and overexpressed in Arabidopsis thaliana. The expression of ScPIP1 at the transcriptional level was induced by polyethylene glycol (PEG) treatment. ScPIP1 overexpressed Arabidopsis plants exhibited higher germination rates, longer roots and higher survival rates compared to the wild-type plants under drought and salt stresses. The results of malonaldehyde (MDA), ion leakage (IL) and proline content measurements indicated that the improved drought and salt tolerance conferred by ScPIP1 was correlated with decreased membrane damage and improved osmotic adjustment. We assume that ScPIP1 may be applied to genetic engineering to improve plant tolerance based on the resistance effect in transgenic Arabidopsis overexpressing ScPIP1.


Assuntos
Aquaporinas/metabolismo , Arabidopsis/metabolismo , Sequência de Aminoácidos , Aquaporinas/classificação , Aquaporinas/genética , Arabidopsis/genética , Secas , Magnoliopsida/genética , Malondialdeído/metabolismo , Fenótipo , Filogenia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Folhas de Planta/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Polietilenoglicóis/farmacologia , Prolina/metabolismo , Tolerância ao Sal , Alinhamento de Sequência , Estresse Fisiológico
8.
Int J Mol Sci ; 19(12)2018 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-30518090

RESUMO

Alternatively translated from the ENO gene and expressed in an array of vertebrate and plant tissues, c-Myc binding protein 1 (MBP-1) participates in the regulation of growth in organisms, their development and their environmental responses. As a transcriptional repressor of multiple proto-oncogenes, vertebrate MBP-1 interacts with other cellular factors to attenuate the proliferation and metastasis of lung, breast, esophageal, gastric, bone, prostrate, colorectal, and cervical cancer cells. Due to its tumor-suppressive property, MBP-1 and its downstream targets have been investigated as potential prognostic markers and therapeutic targets for various cancers. In plants, MBP-1 plays an integral role in regulating growth and development, fertility and abiotic stress responses. A better understanding of the functions and regulatory factors of MBP-1 in plants may advance current efforts to maximize plant resistance against drought, high salinity, low temperature, and oxidative stress, thus optimizing land use and crop yields. In this review article, we summarize the research advances in biological functions and mechanistic pathways underlying MBP-1, describe our current knowledge of the ENO product and propose future research directions on vertebrate health as well as plant growth, development and abiotic stress responses.


Assuntos
Proteínas de Ligação a DNA/metabolismo , Animais , Carcinogênese/metabolismo , Carcinogênese/patologia , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Humanos , Neoplasias/metabolismo , Preparações Farmacêuticas , Desenvolvimento Vegetal , Vertebrados/metabolismo
9.
Mol Biol Rep ; 45(5): 1331-1338, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30120651

RESUMO

There is a growing awareness that some dual-function enzymes may provide a directly evidence that metabolism could feed into the regulation of gene expression via metabolic enzymes. However, the mechanism by which metabolic enzymes control gene expression to optimize plant stress responses remains largely unknown in Arabidopsis thaliana. LOS2/ENO2 is a bifunctional gene transcribed a functional RNA that translates a full-length version of the ENO2 protein and a truncated version of the MBP-1 protein. Here, we report that eno2 negatively regulates plant tolerance to salinity stress. NaCl treatment caused the death of the mutant eno2/eno2 homozygote earlier than the wild type (WT) Arabidopsis. To understand the mechanism by which the mutant eno2 had a lower NaCl tolerance, an analysis of the expressed sequence tag (EST) dataset from the WT and mutant eno2 Arabidopsis was conducted. Firstly, the most identified up- and down-regulated genes are senescence-associated gene 12 (SAG12) and isochorismate mutase-related gene, which are associated with salicylic acid (SA) inducible plant senescence and endogenous SA synthesis, respectively. Secondly, the differentially regulated by salt stress genes in mutant eno2 are largely enriched Gene Ontology(GO) terms associated with various kinds of response to stimulations. Thirdly, in the Kyoto Encyclopedia of Genes and Genomes (KEGG) mapping, we find that knocking out ENO2-influenced genes were most enriched into metabolite synthesis with extra plant-pathogen interaction pathway and plant hormone signal transduction pathway. Briefly, with the translation shifting function, LOS2/ENO2 not only influenced the genes involved in SA synthesis and transduction, but also influenced genes that participate in metabolite synthesis in cytoplasm and gene expression variation in nuclear under salt stress.


Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Regulação da Expressão Gênica de Plantas , Fosfopiruvato Hidratase/genética , Plantas Tolerantes a Sal/genética , Arabidopsis/enzimologia , Proteínas de Arabidopsis/metabolismo , Ácido Corísmico/metabolismo , Cisteína Endopeptidases/metabolismo , Técnicas de Silenciamento de Genes , Fosfopiruvato Hidratase/metabolismo , Ácido Salicílico/metabolismo , Salinidade , Plantas Tolerantes a Sal/enzimologia , Transdução de Sinais , Cloreto de Sódio/farmacologia , Estresse Fisiológico/genética
10.
Yi Chuan ; 39(4): 293-301, 2017 04 20.
Artigo em Inglês | MEDLINE | ID: mdl-28420608

RESUMO

Plasma membrane intrinsic proteins (PIPs) are plant channel proteins located on the plasma membrane. PIPs transfer water, CO2 and small uncharged solutes through the plasma membrane. PIPs have high selectivity to substrates, suggestive of a central role in maintaining cellular water balance. The expression, activity and localization of PIPs are regulated at the transcriptional and post-translational levels, and also affected by environmental factors. Numerous studies indicate that the expression patterns and localizations of PIPs can change in response to abiotic stresses. In this review, we summarize the mechanisms of PIP trafficking, transcriptional and post-translational regulations, and abiotic stress responses. Moreover, we also discuss the current research trends and future directions on PIPs.


Assuntos
Membrana Celular/metabolismo , Proteínas de Membrana/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Aquaporinas/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Regulação da Expressão Gênica de Plantas/fisiologia , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Transporte Proteico/genética , Transporte Proteico/fisiologia , Estresse Fisiológico/genética , Estresse Fisiológico/fisiologia
11.
PLoS One ; 11(4): e0154042, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27097028

RESUMO

Soil salinization is a resource and ecological problem in the world. Thellungiella salsuginea is becoming a new model plant because it resembles its relative species, Arabidopsis thaliana, in small genome and short life cycle. It is highly tolerant to salinity and drought stresses. Ascorbate peroxidase (APX) is an enzyme that clears H2O2 in plants. The function and molecular and regulation mechanisms of APX in T. salsuginea have rarely been reported. In this study, an APX gene, TsApx6, was cloned from T. salsuginea and its responses to abiotic stresses in transgenic Arabidopsis were studied. Under high salinity treatment, the expression of TsApx6 was significantly induced. Under drought treatment, overexpression of TsApx6 increased the survival rate and reduced leaf water loss rate in Arabidopsis. Compared to the wild type plants, high salinity treatment reduced the concentrations of MDA, H2O2 and proline but elevated the activities of APX, GPX, CAT and SOD in the TsApx6-overexpressing plants. Meanwhile, germination rate, cotyledon greening, and root length were improved in the transgenic plants compared to the wild type plants under salt and water deficit conditions. Based on these findings, TsApx6 has an important function in the resistance of plants to certain abiotic stresses. The TsApx6 promoter sequence was obtained using Genome Walking technology. Bioinformatics analysis indicated that it contains some cis-acting elements related to stress response. The treatments of salt, dehydration, and ABA induced the expression of Gus gene under the regulation of the TsApx6 promoter. Mutation analysis showed that the MBS motif present in the TsApx6 promoter might be a key negative regulatory element which has an important effect on the growth and developmental process of plants.


Assuntos
Ascorbato Peroxidases/metabolismo , Brassicaceae/fisiologia , Proteínas de Plantas/metabolismo , Arabidopsis/química , Arabidopsis/genética , Arabidopsis/metabolismo , Arabidopsis/fisiologia , Ascorbato Peroxidases/química , Ascorbato Peroxidases/genética , Brassicaceae/química , Brassicaceae/genética , Secas , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/química , Proteínas de Plantas/genética , Salinidade , Cloreto de Sódio/metabolismo , Estresse Fisiológico
12.
Yi Chuan ; 36(3): 208-19, 2014 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-24846961

RESUMO

Histone modification is one important sort of the epigenetic modifications, including acetylation, formylation, methylation, phosphorylation, ubiquitination and SUMOylation. By forming a complicated network, these modifications control the expression of genes. Histone methylation occurs mainly on the lysine residues, and plays a key role during flowering and stress response of plants, through changing the methylation status of lysine residues and the ratio of methylation. Triple-methylation of H3K4 promotes FLC expression but triple-methylation of H3K27 inhibits its expression. H3K4me3 activates the expression of PtdIns5P gene to initiate lipid synthesis signal pathway in response to drought stress. On the contrary, the low levels of H3K27me3 induce the expression of COR15A and ATGOLS3, which encode for low temperature protective proteins of chloroplast (Cor15am) and Galactional Synthase (GOLS), in order to resist cold stress. In this review, we summarize the molecular mechanisms of histone lysine methylation involved in DNA methylation, plant flowering and stress response.


Assuntos
Regulação da Expressão Gênica de Plantas , Histonas/química , Histonas/metabolismo , Lisina/metabolismo , Plantas/genética , Plantas/metabolismo , Metilação , Desenvolvimento Vegetal/genética
13.
Yi Chuan ; 35(1): 45-54, 2013 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-23357264

RESUMO

Ascorbate peroxidase (APX), a type I heme peroxidase, catalyzes oxidation of ascorbic acid. It possesses a high degree of specificity to ascorbic acid. APX gene cluster consists of four sub-clusters: the gene clusters of cytosol, chloroplast, mitochondria, and peroxidase. As a key component of hydrogen peroxide detoxification system, the ascorbate-glutathione cycle, APX plays a vital role in the metabolism of H2O2 of plant cells. Studies showed that APX is one of the most important enzymes, which modulate the cellular H2O2 level in redox signaling system. The expression mechanisms of APX isoenzymes are quite complex. Briefly, cytosolic APX is regulated by a variety of signals; two chloroplastic APX isoenzymes are tissue-dependently regulated by alternative splicing. Generated APXs could regulate redox signaling in cells, which further boosts plants tolerance to abiotic stresses. This review focuses on recent advances concerning catalytic prop-erties, physiological function, and gene expressing regulation and abio-stress responding mechanism of APX.


Assuntos
Ascorbato Peroxidases/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/metabolismo , Plantas/enzimologia , Ascorbato Peroxidases/genética , Ácido Ascórbico/metabolismo , Oxirredução , Proteínas de Plantas/genética , Plantas/genética , Plantas/metabolismo
14.
Plant Cell ; 24(8): 3235-47, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22892321

RESUMO

Chromatin modifications affect flowering time in the long-day plant Arabidopsis thaliana, but the role of histone methylation in flowering time regulation of rice (Oryza sativa), a short-day plant, remains to be elucidated. We identified a late-flowering long vegetative phase1 (lvp1) mutant in rice and used map-based cloning to reveal that lvp1 affects the SET domain group protein 724 (SDG724). SDG724 functions as a histone methyltransferase in vitro and contributes to a major fraction of global histone H3 lysine 36 (H3K36) methylation in vivo. Expression analyses of flowering time genes in wild-type and lvp1 mutants revealed that Early heading date1, but not Heading date1, are misregulated in lvp1 mutants. In addition, the double mutant of lvp1 with photoperiod sensitivity5 (se5) flowered later than the se5 single mutant, indicating that lvp1 delays flowering time irrespective of photoperiod. Chromatin immunoprecipitation assays showed that lvp1 had reduced levels of H3K36me2/3 at MADS50 and RFT1. This suggests that the divergent functions of paralogs RFT1 and Hd3a, and of MADS50 and MADS51, are in part due to differential H3K36me2/3 deposition, which also correlates with higher expression levels of MADS50 and RFT1 in flowering promotion in rice.


Assuntos
Flores/fisiologia , Histona-Lisina N-Metiltransferase/metabolismo , Histonas/metabolismo , Oryza/enzimologia , Proteínas de Plantas/metabolismo , Sequência de Aminoácidos , Montagem e Desmontagem da Cromatina , Imunoprecipitação da Cromatina , Mapeamento Cromossômico , Clonagem Molecular , Flores/enzimologia , Flores/genética , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Loci Gênicos , Vetores Genéticos , Técnicas de Genotipagem , Histona Metiltransferases , Histona-Lisina N-Metiltransferase/genética , Histonas/genética , Metilação , Dados de Sequência Molecular , Mutação , Oryza/genética , Oryza/fisiologia , Fotoperíodo , Proteínas de Plantas/genética , Fatores de Tempo , Transformação Genética
15.
J Genet Genomics ; 38(3): 95-109, 2011 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-21477781

RESUMO

This article reviews basic concepts, general applications, and the potential impact of next-generation sequencing (NGS) technologies on genomics, with particular reference to currently available and possible future platforms and bioinformatics. NGS technologies have demonstrated the capacity to sequence DNA at unprecedented speed, thereby enabling previously unimaginable scientific achievements and novel biological applications. But, the massive data produced by NGS also presents a significant challenge for data storage, analyses, and management solutions. Advanced bioinformatic tools are essential for the successful application of NGS technology. As evidenced throughout this review, NGS technologies will have a striking impact on genomic research and the entire biological field. With its ability to tackle the unsolved challenges unconquered by previous genomic technologies, NGS is likely to unravel the complexity of the human genome in terms of genetic variations, some of which may be confined to susceptible loci for some common human conditions. The impact of NGS technologies on genomics will be far reaching and likely change the field for years to come.


Assuntos
Genômica/instrumentação , Genômica/métodos , Medicina de Precisão/tendências , Análise de Sequência de DNA/instrumentação , Análise de Sequência de DNA/métodos , Biologia Computacional , Variação Genética , Genômica/economia , Humanos , Nanotecnologia , Alinhamento de Sequência/métodos , Alinhamento de Sequência/tendências , Análise de Sequência de DNA/economia , Software/tendências
16.
Plant Mol Biol ; 76(1-2): 1-18, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21387125

RESUMO

The sweet melon fruit is characterized by a metabolic transition during its development that leads to extensive accumulation of the disaccharide sucrose in the mature fruit. While the biochemistry of the sugar metabolism pathway of the cucurbits has been well studied, a comprehensive analysis of the pathway at the transcriptional level allows for a global genomic view of sugar metabolism during fruit sink development. We identified 42 genes encoding the enzymatic reactions of the sugar metabolism pathway in melon. The expression pattern of the 42 genes during fruit development of the sweet melon cv Dulce was determined from a deep sequencing analysis performed by 454 pyrosequencing technology, comprising over 350,000 transcripts from four stages of developing melon fruit flesh, allowing for digital expression of the complete metabolic pathway. The results shed light on the transcriptional control of sugar metabolism in the developing sweet melon fruit, particularly the metabolic transition to sucrose accumulation, and point to a concerted metabolic transition that occurs during fruit development.


Assuntos
Cucumis melo/genética , Cucumis melo/metabolismo , Perfilação da Expressão Gênica , Sacarose/metabolismo , Análise por Conglomerados , Cucumis melo/crescimento & desenvolvimento , Enzimas/classificação , Enzimas/genética , Enzimas/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Biblioteca Gênica , Redes e Vias Metabólicas/genética , Filogenia , Proteínas de Plantas/classificação , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Solubilidade , Sacarose/química
17.
Radiat Res ; 175(5): 599-609, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21385072

RESUMO

This study evaluated changes in DNA methylation in Arabidopsis thaliana plants grown from seeds implanted with low-energy N(+) and Ar(+) ions. Methylation-sensitive amplified polymorphism (MSAP) testing revealed altered DNA methylation patterns after ion implantation at doses of 1 × 10(14) to 1 × 10(16) ions/cm(2). Comparison of the MSAP electrophoretic profiles revealed nine types of polymorphisms in ion-implanted seedlings relative to control seedlings, among which four represented methylation events, three represented demethylation events, and the methylation status of two was uncertain. The diversity of plant DNA methylation was increased by low-energy ion implantation. At the same time, total genomic DNA methylation levels at CCGG sites were unchanged by ion implantation. Moreover, a comparison of polymorphisms seen in N(+) ion-implanted, Ar(+) ion-implanted, and control DNA demonstrated that the species of incident ion influenced the resulting DNA methylation pattern. Sequencing of eight isolated fragments that showed different changing patterns in implanted plants allowed their mapping onto variable regions on one or more of the five Arabidopsis chromosomes; these segments included protein-coding genes, transposon and repeat DNA sequence. A further sodium bisulfite sequencing of three fragments also displayed alterations in methylation among either different types or doses of incident ions. Possible causes for the changes in methylation are discussed.


Assuntos
Arabidopsis/genética , Arabidopsis/efeitos da radiação , Metilação de DNA/genética , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Sequência de Bases , Análise por Conglomerados , Ilhas de CpG/genética , Ilhas de CpG/efeitos da radiação , Citosina/metabolismo , Epigênese Genética/genética , Epigênese Genética/efeitos da radiação , Loci Gênicos/genética , Loci Gênicos/efeitos da radiação , Genômica , Íons , Mutação/genética , Mutação/efeitos da radiação , Reação em Cadeia da Polimerase , Reprodutibilidade dos Testes , Análise de Sequência de DNA
18.
J Genet Genomics ; 38(1): 29-37, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21338950

RESUMO

Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied. By a large-scale screening of our T-DNA mutant population, we identified a mutant rice lesion initiation 1 (rlin1), which was controlled by a single nuclear recessive gene. Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen III oxidase in tetrapyrrole biosynthesis pathway. Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr. Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype. Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated. These results suggest that tetrapyrrole participates in lesion formation in rice.


Assuntos
Coproporfirinogênio Oxidase/genética , Coproporfirinogênio Oxidase/metabolismo , Necrose/genética , Oryza/citologia , Oryza/genética , Sequência de Aminoácidos , Substituição de Aminoácidos , Mapeamento Cromossômico , Cromossomos de Plantas/genética , Coproporfirinogênio Oxidase/química , Éxons/genética , Regulação da Expressão Gênica de Plantas/efeitos da radiação , Genes Recessivos/genética , Teste de Complementação Genética , Marcadores Genéticos/genética , Humanos , Luz , Dados de Sequência Molecular , Mutação de Sentido Incorreto , Fases de Leitura Aberta/genética , Oryza/enzimologia , Oryza/efeitos da radiação , Fenótipo , Tetrapirróis/biossíntese
19.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 27(4): 423-7, 2010 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-20677150

RESUMO

OBJECTIVE: To investigate the human mitochondrial DNA (mtDNA) variations associated with longevity in Bama elderly population from Guangxi. METHODS: Mitochondrial genome of 20 individuals over 96 years of age was sequenced, and seven target single nucleotide polymorphism (SNPs) were observed by comparing with the standard rCRS sequence, and two were tested by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in a larger population including 208 individuals of 90-113 years old, and 586 unrelated control individuals from Guangxi. RESULTS: The 4824G frequency of the mtDNA4824A/G locus increased with age both in the long-lived elderly and in controls. And it was significantly higher in controls than that in long-lived population (P<0.05). CONCLUSION: The mtDNA4824 A/G is not only an age-related locus, its mutation is also negatively correlated with longevity.


Assuntos
DNA Mitocondrial/análise , Genoma Mitocondrial/genética , Longevidade/genética , Polimorfismo de Nucleotídeo Único/genética , Idoso , China/etnologia , DNA Mitocondrial/genética , Haplótipos , Humanos , Mutação , Mianmar/etnologia , Polimorfismo Genético , Polimorfismo de Fragmento de Restrição , Grupos Populacionais
20.
Mutat Res ; 680(1-2): 64-9, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19815092

RESUMO

In order to study the impact of incident ions on organism mutation, positively and negatively charged low-energy oxygen and hydrogen ions were implanted into the dry seeds of Arabidopsis thaliana using three different dosages. The effects of ion irradiation on peroxidase abundance, activity, isozyme patterns, and transcription were analyzed. The results showed that ion irradiation increases peroxidase activity, transcription, and translation. In all analyses, the relative magnitude of effect of the three ions was consistent, with the implantation of O(+) and H(+) affecting peroxidase more than O(-). This finding suggests that the charge of an incident ion influences cellular systems more than its mass.


Assuntos
Arabidopsis/efeitos da radiação , Íons Pesados/efeitos adversos , Peroxidases/metabolismo , Arabidopsis/enzimologia , Western Blotting , Efeito Espectador/genética , Efeito Espectador/efeitos da radiação , Relação Dose-Resposta à Radiação , Expressão Gênica/efeitos da radiação , Transferência Linear de Energia , Peroxidases/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , RNA Mensageiro/metabolismo , Sementes/genética , Sementes/efeitos da radiação
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