Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 279
Filtrar
1.
Bioresour Technol ; 347: 126678, 2022 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-34999192

RESUMO

Periodic oxygen supplementation (A-O) strategy was proposed to improve pollutant removal and enhance bioresource production of photosynthetic bacteria (PSB). The A-O strategy obtained higher COD (91.4%) and NH4+-N (78.6%) removal compared with the non-oxygen supplementation (N-O) strategy, which was similar to the continuous oxygen supplementation (C-O) strategy. A-O strategy achieved the highest biomass concentration of 1338.5 mg/L. Bacteriochlorophyll and carotenoids concentration in the A-O strategy were 24.9-31.1% and 15.1-23.7% higher than those in the other two strategies; coenzyme Q10 concentration and content were 52.5% and 21.3% higher than that in the N-O strategy. The metabolomic analysis showed that the A-O strategy enhanced the tricarboxylic acid cycle after fumaric acid formation and ß-alanine metabolism, then caused higher biomass accumulation. The A-O strategy reduced the inhibition of photophosphorylation by oxidative-phosphorylation and maintained both characteristics. Hence, A-O might be an economic strategy for enhancing pollutant removal and bioresource production in PSB-based wastewater treatment.

2.
Bioresour Technol ; 343: 126107, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34637910

RESUMO

High light is beneficial for purple non-sulfur bacteria (PNSB) growth. However, excessive light causes photoinhibition. In this novel study, flashing light was used to alleviate photoinhibition and promote biomass growth in PNSB wastewater treatment. Results showed that flashing light effectively increased biomass production. The highest biomass concentration (2688.8 mg/L) and chemical oxygen demand removal (in 177 µmol/m2/s-0.75 duty cycle-1000 Hz group) were 41.5% and 28.4% higher than that in the constant stress light group (same incident light). This group also increased biomass concentration by 21.3% and reduced energy consumption by 26.2% compared with the constant normal light group (same energy input). The shortened single light provision time of flashing light increased the relative electron transportation rate by 116.6%, avoiding photoinhibition, promoting energy utilisation, and enhancing substance synthesis. Flashing light can be used as a light regulation strategy to enhance biomass accumulation and reduce energy consumption in PNSB-based industries.


Assuntos
Proteobactérias , Purificação da Água , Análise da Demanda Biológica de Oxigênio , Biomassa , Luz , Enxofre
3.
J Environ Manage ; 301: 113859, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34597949

RESUMO

In order to strengthen the treatment of low-concentration ammonia nitrogen wastewater at low temperature, iron-loaded activated carbon (Fe-AC) with ultrasonic impregnation method was used as the filter material of biofilter process. The performance and mechanism of ammonia nitrogen removal from simulated secondary wastewater by iron-loaded biological activated carbon filter (Fe-BACF) were studied at 10 °C. The characterization results showed that iron was loaded on the surface of AC in the form of Fe2O3, and the specific surface area, total pore volume, pore size and alkaline functional group content of Fe-AC were obviously increased. After the formation of biofilm on the surface of filter media, the average removal rate of ammonia nitrogen by Fe-BACF (97.9%) was significantly higher than that of conventional BACF (87.8%). The improved surface properties increased the number and metabolic activity of microorganisms, and promoted the secretion of EPS on the surface of Fe-BAC. The results of high-throughput sequencing showed that the existence of Fe optimized the bacterial community structure on the surface of Fe-BAC, with the increase of the abundances of psychrophilic bacteria and ammonia nitrogen removal bacteria. The mechanism of enhanced ammonia nitrogen removal by Fe-BACF was the joint action of many factors, among which the main causal relationship was that modification of iron could optimize the number and category of microorganisms on Fe-BAC surface by improving the surface properties, thus improving the biological nitrogen removal ability. Results of this study provided a practical way for the treatment of low ammonia nitrogen wastewater in cold regions.


Assuntos
Amônia , Águas Residuárias , Amônia/análise , Reatores Biológicos , Carvão Vegetal , Desnitrificação , Filtração , Ferro , Nitrogênio/análise , Temperatura
4.
CNS Neurosci Ther ; 2021 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-34904361

RESUMO

AIMS: Bone morphogenetic proteins (BMPs) are a group of proteins related to bone morphogenesis. BMP-binding endothelial regulator (BMPER), a secreted protein that interacts with BMPs, is known to be involved in ischemic injuries. Here, we explored the effects of BMPER on cerebral ischemia and its mechanism of action. METHODS: A mouse model of brain ischemia was induced by middle cerebral artery occlusion (MCAO). An in vitro ischemic model was established by subjecting primary cultured neurons to oxygen-glucose deprivation/reperfusion (OGD/R). Serum levels of BMPs/BMPER were measured in MCAO mice and in patients with acute ischemic stroke (AIS). Brain damages were compared between BMPER- and vehicle-treated mice. Quantitative polymerase chain reaction (qPCR), immunohistochemistry, and immunofluorescence staining were performed to examine neuroinflammation and cell death. BMPER-related pathways were assessed by Western blotting. RESULTS: BMPER level was elevated in MCAO mice and AIS patients. BMPER administration reduced mortality, infarct size, brain edema, and neurological deficit after MCAO. Neuroinflammation and cell death after ischemia were alleviated by BMPER both in vivo and in vitro. BMPER activated the Smad3/Akt/Nrf2 pathway in OGD/R-challenged neurons. CONCLUSION: BMPER is a neuroprotective hormone that alleviates ischemic brain injury via activating the Smad3/Akt/Nrf2 pathway. These findings may provide potential therapeutic strategies for stroke.

5.
J Hazard Mater ; 424(Pt C): 127554, 2021 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-34736196

RESUMO

Photocatalytic oxidation of contaminants in water has recently gained extensive attentions. In this study, Cu2+-doped BiOCl microsphere photocatalysts were prepared using solvothermal method. The effects of Cu2+ doping ratio on the morphological structures and photoelectric and photocatalytic properties of BiOCl were studied in detail. Results showed that Cu2+ doping affected the particle size of BiOCl microspheres. The introduction of Cu2+ ions gradually increased the light absorption range and decreased the electron recombination rate of photocatalysts as shown by ultraviolet-visible diffuse reflection and photoluminescence spectra. The best doping ratio was 0.25 Cu2+-BiOCl, showing the highest photocatalytic activity for rhodamine B (14.25 time higher than BiOCl) and a good inhibition of algal growth. The main reactants in the photocatalytic system were·OH and h+ (electron holes). Density functional theory (DFT) calculations further demonstrated that the doping of Cu2+ ions made the photogenerated carriers in BiOCl easier to generate and ensured the charge was transferred more rapidly. In conclusion, a novel high-efficiency multifunctional photocatalyst is proposed for the efficient organic pollutants removal and algae growth inhibition from water.

6.
Comput Math Methods Med ; 2021: 4186648, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34795790

RESUMO

Dilated cardiomyopathy (DCM) is a cardiomyopathy with left ventricle or double ventricle enlargement and systolic dysfunction. It is an important cause of sudden cardiac death and heart failure and is the leading indication for cardiac transplantation. Major heart diseases like heart muscle damage and valvular problems are diagnosed using cardiac MRI. However, it takes time for cardiologists to measure DCM-related parameters to decide whether patients have this disease. We have presented a method for automatic ventricular segmentation, parameter extraction, and diagnosing DCM. In this paper, left ventricle and right ventricle are segmented by parasternal short-axis cardiac MR image sequence; then, related parameters are extracted in the end-diastole and end-systole of the heart. Machine learning classifiers use extracted parameters as input to predict normal people and patients with DCM, among which Random forest classifier gives the highest accuracy. The results show that the proposed system can be effectively utilized to detect and diagnose DCM automatically. The experimental results suggest the capabilities and advantages of the proposed method to diagnose DCM. A small amount of sample input can generate results comparable to more complex methods.

8.
Open Med (Wars) ; 16(1): 1378-1385, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34595351

RESUMO

Aim: Renal fibrosis (RF) is a common clinical condition leading to irreversible renal function loss. Tyrosine kinase proteins and microRNAs (miRs) are associated with pathogenesis and we aim to investigate the role of Fer and its partner miR(s) in RF. Method: In silico reproduction of Mouse Kidney FibrOmics browser was performed to identify potential miR(s) and target gene(s). In vivo validation was performed in C57BL/6 mice with unilateral ureteral obstruction (UUO). In vitro validation was performed in rat kidney fibroblast NRK-49F cells. Mimics and inhibitors of miR-29c-3p were constructed. The target gene Fer was monitored by RT-PCR and western blotting. The levels of interleukin (IL)-6, IL-1ß, and tumor necrosis factor (TNF)-α in serum and media were measured by ELISA. Results: The Fer expression and protein level were gradually increased during 14 days of UUO modeling. miR-29c-3p expression was strongly correlated with that of Fer. In vivo validation showed increased expressions of fibrosis-associated genes and increased phospoho-Smad3 level in the UUO model. Fer-knockdown (KD) significantly decreased expressions of fibrosis-associated genes. Pharmaceutical inhibition of Fer showed similar effects to miR-29c-3p, and miR inhibition showed a significant decrease of excretion of inflammatory factors. Conclusion: Dysregulation of miR-29c-3p and Fer plays a role in RF. Pharmaceutical or genetic inhibition of Fer may serve as the potential treatment for RF.

9.
Front Genet ; 12: 729768, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34659354

RESUMO

Background: Although the treatment of cancer has made evident progress, its morbidity and mortality are still high. A tumor marker is a critical indicator for early cancer diagnosis, and timely cancer detection can efficiently help improve the prognosis of patients. Therefore, it is necessary to identify novel markers associated with cancer. LncRNA myocardial infarction associated transcript (MIAT) is a newly identified tumor marker, and in this study, we aimed to explore the relationship between MIAT and clinicopathological features and patient prognosis. Methods: We searched PubMed, Embase, Web of Science, and The Cochrane Library from inception to September 2020 to identify correlational studies. Then, we extracted valid data and used Stata software to make forest plots. We used the hazard ratio (HR) or odds ratio (OR) with 95% CI to evaluate the relationship between aberrant expression of MIAT and patients' prognosis and clinicopathological features. Results: The study included 21 studies, containing 2,048 patients. Meta-analysis showed that overexpression of lncRNA MIAT was associated with poor overall survival (OS) (HR = 1.60, 95% CI, 1.31-1.96, p < 0.001). In addition, high expression of MIAT could forecast tumor size (OR = 2.26, 95% CI 1.34-3.81, p = 0.002), distant metastasis (OR = 2.54, 95% CI 1.84-3.50, p < 0.001), TNM stage (OR = 2.38, 95% CI 1.36-4.18, p = 0.002), lymph node metastasis (OR = 2.59, 95% CI 1.25-5.36, p = 0.011), and the degree of differentiation (OR = 2.65, 95% CI 1.54-4.58, p < 0.001). However, other clinicopathological features, including age (OR = 1.07, 95% CI 0.87-1.32, p = 0.516), gender (OR = 0.95, 95% CI 0.77-1.19, p = 0.668), and histology (OR = 0.72, 95% CI 0.48-1.10, p = 0.128) were not significantly different from high expression of MIAT. Conclusions: Our study showed that overexpression of MIAT is related to poor overall survival and clinicopathological features. MIAT can be considered a novel tumor marker to help diagnose tumors earlier and improve patient prognosis.

10.
Cell Transplant ; 30: 9636897211041585, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34470528

RESUMO

Global cerebral ischemia induced by cardiac arrest usually leads to poor neurological outcomes. Numerous studies have focused on ways to prevent ischemic damage in the brain, however clinical therapies are still limited. Our previous studies revealed that delta opioid receptor (DOR) activation with [d-Ala2, d-Leu5] enkephalin (DADLE), a DOR agonist, not only significantly promotes neuronal survival on day 3, but also improves spatial memory deficits on days 5-9 after ischemia. However, the neurological mechanism underlying DADLE-induced cognitive recovery remains unclear. This study first examined the changes in neuronal survival in the CA1 region at the advanced time point (day 7) after ischemia/reperfusion (I/R) injury and found a significant amelioration of damaged CA1 neurons in the rats treated with DADLE (2.5 nmol) when administered at the onset of reperfusion. The structure and function of CA1 neurons on days 3 and 7 post-ischemia showed significant improvements in both the density of the injured dendritic spines and the basic transmission of the impaired CA3-CA1 synapses following DADLE treatment. The molecular changes involved in DADLE-mediated synaptic modulation on days 3 and 7 post-ischemia implied the time-related differential regulation of PKCα-MARCKS on the dendritic spine structure and of BDNF- ERK1/2-synapsin I on synaptic function, in response to ischemic/reperfusion injury as well as to DADLE treatment. Importantly, all the beneficial effects of DADLE on ischemia-induced cellular, synaptic, and molecular deficits were eliminated by the DOR inhibitor naltrindole (2.5 nmol). Taken together, this study suggested that DOR activation-induced protective signaling pathways of PKCα-MARCKS involved in the synaptic morphology and BDNF-ERK-synapsin I in synaptic transmission may be engaged in the cognitive recovery in rats suffering from advanced cerebral ischemia.

11.
Bioresour Technol ; 342: 126004, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34583109

RESUMO

Anaerobic fermentation of corn straw with rumen microorganisms as inoculum to produce volatile fatty acids (VFAs) is important for biomass valorization. In this study, dynamic variation in bacterial and fungal community composition, carbohydrate-active enzymes (CAZymes) and key functional genes related with VFA production was explored via metagenomic sequencing. Rumen microorganisms efficiently hydrolyzed and acidified corn straw, and VFA concentration reached 8.99 g/L in 72 h. Bacterial and fungal community significantly changed, but the core genera kept stable. Low pH and VFA accumulation were the main factors affecting bacterial and fungal communities. The positive correlations between bacteria were more complex than those between fungi. Most CAZyme abundance significantly decreased after 72 h fermentation, and functional gene abundance participating in VFA generation also decreased. This study provided new insights into dynamic variation of bacteria and fungi during anaerobic ruminal fermentation in vitro, promoting the application of rumen microorganisms in practice.

12.
Bioengineered ; 12(1): 4768-4779, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34348577

RESUMO

Nitrous Oxide (N2O) has been shown to be neurotoxic, but its specific mechanism is still unclear. The purpose of this work is to probe into the impact of N2O on nerve cell injury through regulating thioredoxin-interacting protein (TXNIP)/the NOD-like receptor domain of pyrin containing 3 (NLRP3) pathway. The results indicated that, N2O exposure elevated TXNIP/NLRP3 expression in vivo and in vitro, led to declined learning and memory capabilities in mice, reduced apoptosis rate in hippocampal neuron and Nissl bodies, elevated inflammatory factors TNF-α, IL-1ß and IL-6 levels, as well as cleaved caspase-3 and Bax expressions, and reduced Bcl-2 expression. Overexpressing TXNIP or NLRP3 further aggravated these injuries, but knocking down TXNIP or NLRP3 improved them. CO-IP indicated that TXNIP and NLRP3 can be combined, with interaction relationship. All in all, the results manifested that N2O is available to promote nerve cell inflammation and apoptosis through activating the TXNIP/NLRP3 pathway that can be used as a potential target for N2O-induced nerve damage in the future.

13.
Oxid Med Cell Longev ; 2021: 8869908, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34373769

RESUMO

Delayed ischemic neurological deficit (DIND) is a severe complication after subarachnoid hemorrhage (SAH). Previous studies have suggested that bilirubin oxidation end products (BOXes) are probably associated with the DIND after SAH, but there is a lack of direct evidence yet even on cellular levels. In the present study, we aim to explore the potential role of BOXes and the involved mechanisms in neuronal function. We synthesized high-purity (>97%) BOX A and BOX B isomers. The pharmacokinetics showed they are permeable to the blood-brain barrier. Exposure of a moderate concentration (10 or 30 µM) of BOX A or BOX B to isolated primary cortical neurons increased the production of reactive oxygen species. In the human neuroblastoma SH-SY5Y cells, BOX A and BOX B decreased the mitochondrial membrane potential and enhanced nuclear accumulation of the protein Nrf2 implicated in oxidative injury repair. In addition, both chemicals increased the mRNA and protein expression levels of multiple antioxidant response genes including Hmox1, Gsta3, Blvrb, Gclm, and Srxn1, indicating that the antioxidant response element (ARE) transcriptional cascade driven by Nrf2 is activated. In conclusion, we demonstrated that primary cortical neurons and neuroblastoma cells undergo an adaptive response against BOX A- and BOX B-mediated oxidative stress by activation of multiple antioxidant responses, in part through the Nrf2 pathway, which provides in-depth insights into the pathophysiological mechanism of DIND after SAH or other neurological dysfunctions related to cerebral hemorrhage.

14.
World J Microbiol Biotechnol ; 37(9): 161, 2021 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-34436687

RESUMO

Shortage of water, energy, and bioresources in the world has led to the exploration of new technologies that achieve resource recovery from wastewater, which has become a new sustainable trend. Photosynthetic non-sulfur bacteria (PNSB), the most ancient photo microorganism, not only treats different wastewater types, but also generates PNSB cells, which are non-toxic bioresources and containing many value-added products. These bioresources can be used as raw materials in the agricultural, food, and medical industries. Therefore, PNSB or PNSB-based wastewater resource recovery technology can be simultaneously used to treat wastewater and produce useful bioresources. Compared with traditional wastewater treatment, this technology can reduce CO2 emissions, promote the N recovery ratio and prevent residual sludge disposal or generation. After being developed for over half a century, PNSB wastewater resource recovery technology is currently extended towards industrial applications. Here, this technology is comprehensively introduced in terms of (1) PNSB characteristics and metabolism; (2) PNSB wastewater treatment and bioresource recovery efficiency; (3) the relative factors influencing the performance of this technology, including light, oxygen, strains, wastewater types, hydraulic retention time, on wastewater treatment, and resource production; (4) PNSB value-added bioresources and their generation from wastewater; (5) the scale-up history of PNSB technology; (6) Finally, the future perspectives and challenges of this technology were also analysed and summarised.

15.
Sci Prog ; 104(3): 368504211031114, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34261389

RESUMO

Sound quality (SQ) has become an important index to measure the competitiveness of motor products. To better evaluate and optimize SQ, a novelty SQ evaluation and prediction model of high-speed permanent magnet motor (HSPMM) with better accuracy is presented in this research. Six psychoacoustic parameters of A-weighted sound pressure level (ASPL), loudness, sharpness, roughness, fluctuation strength (FS), and perferred-frequency speech interference (PSIL) were adopted to objectively evaluate the SQ of HSPMM under multiple operating conditions and subjective evaluation was also conducted by the combination of semantic subdivision method and grade scoring method. The evaluation results show that the SQ is poor, which will have a certain impact on human psychology and physiology. The correlation between the objective evaluation parameters and the subjective scores is analyzed by coupling the subjective and objective evaluation results. The average error of multiple linear regression (MLR) model is 7.10%. It has good accuracy, but poor stability. In order to improve prediction accuracy, a new predicted model of radial basis function (RBF) artificial neural network was put forward based on genetic algorithm (GA) optimization. Compared with MLR, its average error rate is reduced by 3.16% and the standard deviation is reduced by 1.841. In addition, the weight of each objective parameter was analyzed. The new predicted model has a better accuracy. It can evaluate and optimize the SQ exactly. The research methods and conclusions of this paper can be extended to the evaluation, prediction, and optimization of SQ of other motors.

16.
Front Bioeng Biotechnol ; 9: 615090, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34307314

RESUMO

Background: Cardiac conduction disturbance requiring new permanent pacemaker implantation (PPI) is an important complication of TAVR that has been associated with increased mortality. It is extremely challenging to optimize the valve size alone to prevent a complete atrioventricular block (AVB). Methods: In this study, we randomly took 48 patients who underwent TAVR and had been followed for at least 2 years to assess the risk of AVB. CT images of 48 patients with TAVR were analyzed using three-dimensional (3D) anatomical models of the aortic valve apparatus. The stresses were formulated according to loading force and tissue properties. Support vector regression (SVR) was used to model the relationship between AVB risk and biomechanical stresses. To avoid AVB, overlapping regions on the prosthetic valve where AV bundle passes will be removed as cylindrical sector with the angle θ. Thus, the optimization of the valve shape will be predicted with the joint optimization of the θ and valve size R. Results: The average AVB risk prediction accuracy was 83.33% in the range from 0.8-0.85 with 95% CI for all cases; specifically, 85.71% for Group A (no AVB), and 80.0% for Group B (undergoing AVB after the TAVR). Conclusions: This model can estimate the optimal valve size and shape to avoid the risk of AVB after TAVR. This optimization may eliminate the excessive stresses to keep the normal function of both AV bundle and valve leaflets, leading to a favorable clinical outcome. The combination of biomechanical properties and machine learning method substantially improved prediction of surgical results.

17.
Front Aging Neurosci ; 13: 692942, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34326767

RESUMO

Objective: The aim of this study was to perform a systematic review and meta-analysis to assess whether cerebral small vessel disease (CSVD) on neuroimaging of patients with acute ischemic stroke (AIS) treated with intravenous thrombolysis (IVT) is associated with an increased risk of hemorrhagic transformation (HT), symptomatic intracranial hemorrhage (sICH), and poor functional outcome (PFO). Methods: A thorough search of several databases was carried out to identify relevant studies up to December 2020. We included studies of patients with AIS and neuroimaging markers of CSVD treated with IVT. The primary outcome was HT, and the secondary outcomes were sICH and 3-month PFO. The quality of the studies involved was evaluated using the Newcastle-Ottawa Scale (NOS). The meta-analysis with the fixed effects model was performed. Results: Twenty-four eligible studies (n = 9,419) were pooled in the meta-analysis. All included studies were regarded as high quality with the NOS scores of at least 6 points. The meta-analysis revealed associations between the presence of CSVD and HT, sICH, and the 3-month PFO after IVT. Compared with no CSVD, the presence of CSVD was associated with an increased risk of HT (OR: 1.81, 95% CI: 1.52-2.16), sICH (OR: 2.42, 95% CI: 1.76-3.33), and 3-month PFO (OR: 2.15, 95% CI: 1.89-2.44). For patients with AIS complicated with CSVD, compared with a CSVD score of 0-1, a CSVD score of 2-4 was associated with an increased risk of HT (OR: 3.10, 95% CI: 1.67-5.77), sICH (OR: 2.86, 95% CI: 1.26-6.49), and 3-month PFO (OR: 4.58, 95% CI: 2.97-7.06). Conclusion: Patients with AIS complicated with neuroimaging markers of CSVD are at increased risk of HT and 3-month PFO after IVT. However, it is still necessary to clarify the exact role of CSVD in the occurrence, development, and prognosis of AIS. Systematic Review Registration: www.ClinicalTrials.gov, identifier CRD4202123 3900.

18.
Aging (Albany NY) ; 13(14): 19028-19047, 2021 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-34315829

RESUMO

BACKGROUND: Autophagy, a process of self-digestion, is closely related to multiple biological processes of colon cancer. This study aimed to construct and evaluate prognostic signature of autophagy-related genes (ARGs) to predict overall survival (OS) in colon cancer patients. MATERIALS AND METHODS: First, a total of 234 ARGs were downloaded via The Cancer Genome Atlas (TCGA) database. Based on the TCGA dataset, differentially expressed ARGs were identified in colon cancer. The univariate and multivariate Cox regression analysis was performed to screen prognostic ARGs to construct the prognostic model. The feasibility of the prognostic model was evaluated using receiver operating characteristic curves and Kaplan-Meier curves. A prognostic model integrating the gene signature with clinical parameters was established with a nomogram. RESULTS: We developed an autophagy risk signature based on the 6 ARGs (ULK3, ATG101, MAP1LC3C, TSC1, DAPK1, and SERPINA1). The risk score was positively correlated with poor outcome and could independently predict prognosis. Furthermore, the autophagy-related signature could effectively reflect the levels of immune cell type fractions and indicate an immunosuppressive microenvironment. CONCLUSION: We innovatively identified and validated 6 autophagy-related gene signature that can independently predict prognosis and reflect overall immune response intensity in the colon cancer microenvironment.

19.
ACS Appl Mater Interfaces ; 13(30): 36295-36306, 2021 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-34293853

RESUMO

Cylindrical microlens arrays (CMLAs) play a key role in many optoelectronic devices, and 100% fill-factor CMLAs also have the advantage of improving the signal-to-noise ratio and avoiding stray-light effects. However, the existing preparation technologies are complicated and costly, which are not suitable for mass production. Herein, we propose a simple, efficient, and low-cost manufacturing method for CMLAs with a high fill-factor via the electric-field-driven (EFD) microscale 3D printing of polydimethylsiloxane (PDMS). By adjusting the printing parameters, the profile and the fill-factor of the CMLAs can be controlled to improve their optical performance. The optical performance test results show that the printed PDMS CMLAs have good image-projecting and light-diffraction properties. Using the two printing modes of this EFD microscale 3D-printing technology, a cylindrical dual-microlens array with a double-focusing function is simply prepared. At the same time, we print a series of specially shaped microlenses, proving the flexible manufacturing capabilities of this technology. The results show that the prepared CMLAs have good morphology and optical properties. The proposed method may provide a viable route for manufacturing large-area CMLAs with 100% fill-factor in a very simple, efficient, and low-cost manner.

20.
Bioresour Technol ; 337: 125362, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34116280

RESUMO

So as to accelerate the resuscitation of starved anaerobic granular sludge after long-term stagnation, an innovative method was tried derived from the regulation of N-acyl-homoserine lactones (AHLs)-mediated quorum sensing (QS). The mixture of four AHLs was added to the starved anaerobic granular sludge system in this research. The results confirmed that the exogenous AHLs shortened the recovery time of the granular sludge, and improved the treatment performance and methanogenic capacity of the recovered anaerobic sludge to the level before stagnation. At the same time, exogenous AHLs enhanced the synthesis of extracellular polymeric substances (EPS) during the resuscitation period of starved anaerobic granular sludge. The outcomes of microbial composition detection showed that the change of bacterial and methanogenic bacteria communities towards accelerated performance recovery was significantly correlated with exogenous AHLs. This exploration provided a new technical idea for speeding up the recovery of starved anaerobic granular sludge.


Assuntos
Acil-Butirolactonas , Esgotos , Anaerobiose , Matriz Extracelular de Substâncias Poliméricas , Lactonas , Percepção de Quorum
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...