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1.
Geriatr Gerontol Int ; 2021 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-34850514

RESUMO

AIMS: This study aimed to explore the safety and efficacy of endoscopic retrograde cholangiopancreatography (ERCP) in patients over 90 years of age. METHODS: The study included 176 patients aged over 85 years who received their ERCP from February 2002 to January 2021. In the case group (super-elderly group), 44 patients were 90 years old or above. In the control group (younger group), there were 132 patients aged 85-89 years. The control group was matched according to patient gender and the same indications of ERCP at a 1:3 ratio. Logistic regression models were employed to evaluate all complications. RESULTS: The case group had higher acute physiology and chronic health evaluation scoring system (APACHE-II) scores and rate of hypoalbuminemia. APACHE-II scores (≥6 or 7) were significantly more common in the case group. The rates of technical success and complete success in the case group were 100% and 100% respectively, which were similar to the rates in the control group, namely a technical success rate of 98.5% and a complete success rate of 98.5%. The rate of complication in the case group was 9.1%, which was slightly lower than that of the control group (15.2%, P > 0.05). ERCP-related death occurred in one patient in the control group, who had malignant biliary obstruction and died from cholangitis. There was no significant difference in the incidence of complications such as pancreatitis, hemorrhage, and infection between the two groups. In the multivariate analysis, the independent risk factor was Charlson Comorbidity Index (CCI) for overall complication. CONCLUSIONS: ERCP can be performed safely and successfully in patients aged ≥90 years. Geriatr Gerontol Int 2021; ••: ••-••.

2.
Anticancer Drugs ; 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34459456

RESUMO

Melanoma is a malignant form of cutaneous cancer with an increasing incidence since 1970s, accounting for nearly 75% of the death related to skin cancer especially in western countries. Highest recurrence and mortality were observed for the subtype with distal metastasis, demonstrating poor outcomes. However, high incidence of gastrointestinal metastasis of malignant melanoma is frequently misdiagnosed due to lack of specific clinical manifestations, especially for the rare observed cases presented amelanotic appearance, accounting for about 2% of all metastatic cases. In the present study, we reported a 36-year-old male patient, who was firstly diagnosed as gastric cancer, and then was confirmed as amelanotic melanoma metastasis by pathological examination, demonstrating positive for melanoma markers including Melan A, S-100, Hmb45 and CD79a. In conclusion, for the amelanotic neoplasm observed during gastroscopy in patients with melanoma history, pathological examination should be carried out to confirm the possibility of melanoma metastasis, providing evidences for the following treatment.

3.
Appl Bionics Biomech ; 2021: 9986395, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34335875

RESUMO

Craniocerebral injury has been a research focus in the field of injury biomechanics. Although experimental endeavors have made certain progress in characterizing the material behavior of the brain, the temperature dependency of brain mechanics appears to be inconclusive thus far. To partially address this knowledge gap, the current study measured the brain material behavior via unconstrained uniaxial compression tests under low strain rate (0.0083 s-1) and high strain rate (0.83 s-1) at four different sample temperatures (13°C, 20°C, 27°C, and 37°C). Each group has 9~12 samples. One-way analysis of variance method was used to study the influence of sample temperature on engineering stress. The results show that the effect of sample temperature on the mechanical properties of brain tissue is significant under the high strain rate, especially at low temperature (13°C), in which the hardening of the brain tissue is very obvious. At the low strain rate, no temperature dependency of brain mechanics is noted. Therefore, the current results highlight that the temperature of the brain sample should be ensured to be in accordance with the living subject when studying the biomechanical response of living tissue.

4.
Ann Biomed Eng ; 49(7): 1747-1760, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33479788

RESUMO

The 0.2% strain offset approach is mostly used to calculate the yield stress and serves as an efficient method for cross-lab comparisons of measured material properties. However, it is difficult to accurately determine the yield of the bone. Especially when computational models require accurate material parameters, clarification of the yield point is needed. We tested 24 cortical specimens harvested from six bovine femora in three-point bending mode, and 11 bovine femoral cortical specimens in the tensile mode. The Young's modulus and yield stress for each specimen derived from the specimen-specific finite element (FE) optimization method was regarded as the most ideal constitutive parameter. Then, the strain offset optimization method was used to find the strain offset closest to the ideal yield stress for the 24 specimens. The results showed that the 0 strain offsets underestimated (- 25%) the yield stress in bending and tensile tests, while the 0.2% strain offsets overestimated the yield stress (+ 65%) in three-point bending tests. Instead, the yield stress determined by 0.007 and 0.05% strain offset for bending and tensile loading respectively, can effectively characterize the biomechanical responses of the bone, thereby helping to build an accurate FE model.

5.
J Biomech Eng ; 143(2)2021 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-32839823

RESUMO

The bending fracture behaviors of long bone have gained great attention due to the high bending fracture risk during sports events, traffic accidents, and falling incidents, etc. For evaluating bone bending behaviors, most of the previous studies used an indenter in three point bending experiments while the effect of its rigidity was never considered. In this work, using the porcine long bones, the three point bending tests were conducted to explore the bone fracture behaviors under a rigid indenter. In addition to collecting the force applied, the bone fracture dynamic process was recorded by high-speed photography, and the fracture surface profile in mesoscale was observed by the scanning electron microscope (SEM). Based on CT scanning of long bones, the cross section properties of test specimens were calculated by a homemade matlab script for correlating with their failure strengths. Also, a subject-specific finite element (FE) model was developed to identify the outcomes induced by a rigid indenter on simulation. Findings led to conclusions as follows: (1) The tension fracture came with fracture path deflection, which was caused by the bone indentation induced mesoscale crack-opening. Due to this damage before the whole bone fracture, a bone fracture moment correction was established to compensate experimental data. (2) The plastic indentation caused the force fluctuation as suggested by correlation analysis. (3) The bone failure moment correlated with the inertial moment of the bone cross section at the fracture location higher than the traditional cross section area. (4) In the subject-specific simulation, the indentation caused compression fracture under a much lower failure force. Removing the element erosion on the indenter-contacted area only during the validation was verified as a good option to solve this issue.

6.
Int J Gynaecol Obstet ; 153(3): 405-411, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33230808

RESUMO

OBJECTIVE: To analyze the clinical profile and prognosis of primary Fallopian tube cancer (PFTC) in order to improve earlier diagnosis. METHODS: In this retrospective study, 57 women with PFTC were assessed from 2006 to 2016. Pathology, clinical index, recurrence, and survival were analyzed. RESULTS: Mean age was 57.35 ± 9.01 years, and 73% (19/26) of the patients with early-stage PFTC (I/II) were aged less than 60 years. Of patients who presented with abnormal vaginal bleeding, 75% (9/12) were at an early stage and their condition was often misdiagnosed as endometrial carcinoma preoperatively. In patients with Stages I/II and Stages III/IV PFTC, 59.09% (13/22) and 96.43% (27/28), respectively, had adnexal masses on color Doppler ultrasonography. The 5-year overall survival (OS) and disease-free survival rates were 69.23% and 44.23%, respectively, and univariate analysis showed that tumor stage and residual tumor size significantly affected the two survival rates. CONCLUSION: Primary Fallopian tube cancer is more likely to be misdiagnosed in patients aged less than 60 years or those presenting with vaginal bleeding at the premenopausal stage. Magnetic resonance imaging, cervical smear, and endometrial brush may be helpful for early PFTC diagnosis. Satisfactory cytoreductive surgery is critical because tumor stage and residual tumor size are significantly associated with the OS rate.


Assuntos
Carcinoma/mortalidade , Carcinoma/patologia , Neoplasias das Tubas Uterinas/mortalidade , Neoplasias das Tubas Uterinas/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígeno Ca-125/sangue , Carcinoma/diagnóstico por imagem , Carcinoma/terapia , Erros de Diagnóstico , Intervalo Livre de Doença , Neoplasias das Tubas Uterinas/diagnóstico por imagem , Neoplasias das Tubas Uterinas/terapia , Feminino , Humanos , Imageamento por Ressonância Magnética , Proteínas de Membrana/sangue , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Estudos Retrospectivos , Taxa de Sobrevida , Tomografia Computadorizada por Raios X , Ultrassonografia Doppler em Cores , Hemorragia Uterina/etiologia
7.
J Cell Mol Med ; 24(21): 12608-12618, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32951327

RESUMO

We previously demonstrated that cancer-associated fibroblasts (CAFs) promoted the proliferation of gallbladder cancer (GBC) cells, but the mechanism is not clear. Neuropilin-1 (NRP-1) plays an important role in various malignancies as transmembrane glycoprotein. Our goal was to reveal the relationship between CAFs and NRP-1 and their potential functions in GBC. In this study, we found NRP-1 was overexpressed in GBC tissue, associated with poor survival and was up-regulated by CAFs. The cytokine array cluster analysis revealed IL-8 secreted by CAFs facilitated the up-regulation of NRP-1 in tumour cells. NRP-1 knockdown suppressed tumour growth in vivo. Gene expression microarray analysis showed 581 differentially regulated genes under NRP-1 knockdown conditions. Ingenuity pathway analysis demonstrated that NRP-1 knockdown may inhibit tumour progression by affecting cell proliferation. We then confirmed that NRP-1 knockdown in NOZ and GBC-SD cells significantly inhibited cell proliferation. Additionally, the IL-8 mediated MDM2 and CCNA2 expression were affected by NRP-1 knockdown. Our findings suggested that NRP-1 was up-regulated by CAF-secreted IL-8, which subsequently promoted GBC cell proliferation, and these molecules may serve as useful prognostic biomarkers and therapeutic targets for GBC.


Assuntos
Fibroblastos Associados a Câncer/metabolismo , Fibroblastos Associados a Câncer/patologia , Neoplasias da Vesícula Biliar/genética , Neoplasias da Vesícula Biliar/patologia , Regulação Neoplásica da Expressão Gênica , Interleucina-8/metabolismo , Neuropilina-1/genética , Regulação para Cima/genética , Animais , Linhagem Celular Tumoral , Proliferação de Células , Colecistite/genética , Feminino , Humanos , Masculino , Camundongos Nus , Pessoa de Meia-Idade , Análise Multivariada , Análise de Sequência com Séries de Oligonucleotídeos , Prognóstico , RNA Interferente Pequeno/metabolismo , Análise de Sobrevida , Ensaio Tumoral de Célula-Tronco
8.
Cancer Sci ; 111(11): 4075-4087, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32860308

RESUMO

Suppressor of Ty 16 (Spt16) is a component of the facilitates chromatin transcription (FACT) complex, which is a histone chaperone and involved in gene transcription, DNA replication, and DNA repair. Previous studies showed that FACT is highly expressed in cancer, and cancer cells are more reliant on FACT than normal cells. However, the relationship between Spt16 and lung cancer remains unclear. In this study, we explored the functions of Spt16 in lung cancer cells. The effects of Spt16 on lung cancer cell proliferation, cell cycle progression, apoptosis, migration, and invasion were examined. We found that knockdown of Spt16 led to obvious decreases of both Rb and MCM7, and further activated the DNA damage response (DDR) pathway. In addition, a novel micro-RNA, miR-1227-5p, directly targeted the 3'-UTR of Spt16 and regulated the mRNA levels of Spt16. Furthermore, we found that CBL0137, the functional inhibitor of FACT, showed similar effects as loss of Spt16. Together, our data indicated that Spt16 is likely to be an essential regulator for lung cancer malignancy and is negatively regulated by miR-1227-5p.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , MicroRNAs/genética , Fatores de Transcrição/metabolismo , Regiões 3' não Traduzidas , Apoptose/genética , Carbazóis/farmacologia , Ciclo Celular/genética , Cromatina/genética , Cromatina/metabolismo , Dano ao DNA , Progressão da Doença , Técnicas de Silenciamento de Genes , Humanos , Neoplasias Pulmonares/patologia , Gradação de Tumores , Interferência de RNA , Transcrição Genética/efeitos dos fármacos
9.
Phys Chem Chem Phys ; 22(39): 22207-22216, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32807994

RESUMO

Polymers, especially polyethylene (PE), are widely employed as insulating materials in electrical power transmission systems. However, the insulation still faces the problem of space charge, which distorts the electric field distribution and accelerates electrical aging. Experimental results show that after the fluorination process, less charge injection occurs compared with pure PE. To clarify the mechanism, classical molecular dynamics was employed to build a PE/fluorinated layer interfacial model and first principles calculation was utilized to get the band offset at the interface. The results calculated by both the bulk plus band lineup method and the layer-decomposed density of states method show that the energy band of the fluorinated PE layer is overall lower than that of the PE side, and the band offsets are around 2 eV. Charge transport results based on Marcus theory and kinetic Monte Carlo simulations also show that charge can easily accumulate at the interfacial area under an electric field and the band offset can suppress charge injection. The conduction band offset acts as an energy barrier for the excess electrons at the fluorinated layer side to cross the interface, while the valence band offset has the same effect on hole transport because of the energy barrier caused by the inverted region. Our findings provide a fundamental and theoretical basis for material modification and space charge inhibition.

10.
Polymers (Basel) ; 12(6)2020 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-32471091

RESUMO

In order to realize the diagnosis of water distribution, this paper analyzes the interface polarization and macroscopic space charge polarization mechanism when the water distribution is non-uniform. The experimental results of this paper and bushing show that when the moisture distribution is non-uniform, there is a significant loss peak in the tanδ-f curve. The loss peak shifts to higher frequencies as the non-uniformity coefficient increases. There are common intersection points between multiple tanδ-f curves. Further, this paper realizes the diagnosis of the location of moisture distribution through Frequency Domain Spectroscopy (FDS) testing of different voltages and different wiring methods based on the macroscopic space charge polarization. In the single-cycle FDS test, when the positive electrode is first added to the area with higher moisture content, the amplitude of the tanδ-f curve is smaller. The tanδ-f curves under different wiring methods constitute a "ring-shaped" loss peak. As the voltage increases, the peak value of the loss peak shifts to the lower frequency band. As the temperature increases, the peak value of the loss peak shifts to higher frequencies. Based on the above rules and mechanism analysis, this research provides a new solution for the evaluation of moisture content of oil-immersed polymers equipment.

11.
Exp Mol Med ; 52(5): 854-864, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32467608

RESUMO

Tumor-promoting inflammation is a hallmark of cancer and is highly associated with tumor progression, angiogenesis, and metastasis. Tumor-associated macrophages (TAMs) are major drivers of tumor-promoting inflammation, but due to the complexity of the tumor microenvironment, the detailed regulatory mechanisms are still under investigation. Here, we investigated a novel role for transglutaminase 2 (TGM2) in the development of tumor-promoting inflammation and recruitment of TAMs to gastric cancer (GC) tissues. When estimated by array comparative genomic hybridization and droplet digital PCR, the copy numbers of the TGM2 gene were amplified in 13.6% (14/103) of GC patients and positively associated with TGM2 expression. Gene set enrichment analysis of expression microarray data for GC samples with high or low TGM2 expression showed that increased TGM2 expression was associated with tumor-promoting inflammation in GC. In addition, the expression of TGM2 was correlated with the expression of markers for macrophages, neutrophils, blood vessels, and lymphatic vessels. Overexpression of TGM2 in GC cells augmented the IL-1ß-induced secretion of macrophage-recruiting chemokines and NF-κB activation. TGM2 protein levels were associated with the expression levels of the macrophage marker CD163 in human GC tissue samples. Moreover, GC patients with high expression of TGM2 had a worse prognosis than those with low expression of TGM2. These results suggest TGM2 as a novel regulator of the tumor microenvironment of GC and provide a promising target for constraining tumor-promoting inflammation.


Assuntos
Proteínas de Ligação ao GTP/genética , Amplificação de Genes , Inflamação/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Transglutaminases/genética , Linhagem Celular Tumoral , Biologia Computacional/métodos , Proteínas de Ligação ao GTP/metabolismo , Dosagem de Genes , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Inflamação/metabolismo , Inflamação/patologia , Prognóstico , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/mortalidade , Transcriptoma , Transglutaminases/metabolismo , Microambiente Tumoral/genética , Macrófagos Associados a Tumor/imunologia , Macrófagos Associados a Tumor/metabolismo , Macrófagos Associados a Tumor/patologia
12.
Nanotechnology ; 31(36): 364002, 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32413876

RESUMO

Surface flashover properties of alumina/epoxy spacers, involving a surface charge accumulation process, are critical for the safe and reliable operation of a high-voltage direct-current (HVDC) gas-insulated transmission line (GIL). This study reports surface charging behavior and flashover performance of alumina/epoxy spacers with different surface conductivity graded coating (SCGC) schemes in SF6/N2 mixtures under DC stress. Four kinds of SCGC schemes, i.e. localized coating near high voltage (HV-coating), near grounded electrode (GND-coating), at the middle of spacer surface (SPM-coating) and near both high voltage and grounded electrode (HV-GND-coating), are designed by partially spraying SiC/epoxy composites on the spacer surface. Surface charge distribution patterns exhibit varied features with different SCGC schemes. The HV-coating and GND-coating schemes lead to aggravated homo-charge and hetero-charge accumulation respectively, whereas in the SPM-coating scheme surface charge shows a multi-tier distribution pattern with alternating polarity. A transition of the dominant surface charge mechanism from bulk conductivity to surface conductivity with increasing conductivity on the coated area is found. Flashover performance differs a lot with different SCGC schemes: the HV-coating and HV-GND-coating schemes increase the flashover voltage while the SPM-coating and GND-coating schemes degrade it. The optimal surface insulation strength is achieved in the HV-coating scheme with a coating width of about 10 mm. The impact of different SCGC schemes on flashover performance is revealed based on the electric field analysis by considering the effect of surface charges.

13.
Pathol Res Pract ; 216(6): 152926, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32327282

RESUMO

Recent studies showed that the tumor-infiltrating lymphocytes (TILs) are not randomly distributed, but organized to accumulate more or less densely in different regions within tumors, which have provoked new thoughts on cancer management. In this study we explored the characteristics of tumor immunemicroenvironment (TIME) for the tumor budding (TB) and lymphocytes in patients with gastric adenocarcinoma (GAC) as well as their prognostic significance. The TILs around the TB at the invasive margin were assessed by double-immunohistochemistry staining for the CD8, FOXP3, OX40 and GrB phenotypes. Results showed that there was a negative correlation between the density of TB and TILs in the budding area, tumor stroma and parenchyma. And the number of TILs around the TB was evidently reduced, compared with TILs in the non-budding region (P < 0.001). Additionally, the number of TILs in turn changed from non-budding area CD8+>FOXP3+>OX40+> GrB + T cells to FOXP3+>CD8+>OX40 + T > GrB + T cells in budding area. Survival rate was significantly lower in patients who had a higher density of TB (P < 0.001) and a lower density of TILs (P = 0.013). We concluded that TB was surrounded by a weak immune surveillance and immunosuppressive response supported the spatial heterogeneity in the TIME of gastric adenocarcinomas. The regional heterogeneity should be attached importance for identifying the influence of the TIME on cancer development and evolution.


Assuntos
Adenocarcinoma/imunologia , Linfócitos do Interstício Tumoral/imunologia , Neoplasias Gástricas/imunologia , Microambiente Tumoral/imunologia , Adenocarcinoma/patologia , Adulto , Idoso , Feminino , Humanos , Linfócitos do Interstício Tumoral/patologia , Masculino , Pessoa de Meia-Idade , Prognóstico , Neoplasias Gástricas/patologia
14.
Phys Rev E ; 101(3-1): 033203, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32289891

RESUMO

The plasma sheath is the non-neutral space charge region that isolates bulk plasma from a boundary. Radio-frequency (RF) sheaths are formed when applying RF voltage to electrodes. Generally, applied bias is mainly consumed by a RF sheath, which shields an external field. Here we report evidence that an intense boundary emission destroys a normal RF sheath and establishes a type of RF plasma where external bias is consumed by bulk plasma instead of a sheath. Ions are naturally confined while plasma electrons are unobstructed, generating a strong RF current in the entire plasma, combined with a unique particle and energy balance. The proposed model offers the possibility for ion erosion mitigation of a plasma-facing component. It also inspires techniques for reaction rate control in plasma processing and wave mode conversion.

15.
Mol Genet Genomic Med ; 8(5): e1209, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32163678

RESUMO

BACKGROUND: Shaanxi province, located in the upper Yellow River, has been evidenced as the geographic origin of Chinese civilization, Sino-Tibetan-speaking language, and foxtail or broomcorn millet farmers via the linguistic phylogenetic spectrum, archeological documents, and genetic evidence. Nowadays, Han Chinese is the dominant population in this area. The formation process of modern Shaanxi Han population reconstructed via the ancient DNA is on the way, however, the patterns of genetic relationships of modern Shaanxi Han, allele frequency distributions of high mutated short tandem repeats (STRs) and corresponding forensic parameters are remained to be explored. METHODS: Here, we successfully genotyped 23 autosomal STRs in 630 unrelated Shaanxi male Han individuals using the recently updated Huaxia Platinum PCR amplification system. Forensic allele frequency and parameters of all autosomal STRs were assessed. And comprehensive population genetic structure was explored via various typical statistical technologies. RESULTS: Population genetic analysis based on the raw-genotype dataset among 15,803 Eurasian individuals and frequency datasets among 56 populations generally illustrated that linguistic stratification is significantly associated with the genetic substructure of the East Asian population. Principal component analysis, multidimensional scaling plots and phylogenetic tree further demonstrated that Shaanxi Han has a close genetic relationship with geographically close Shanxi Han, and showed that Han Chinese is a homogeneous population during the historic and recent admixture from the STR variations. Except for Sinitic-speaking populations, Shaanxi Han harbored more alleles sharing with Tibeto-Burman-speaking populations than with other reference populations. Focused on the allele frequency correlation and forensic parameters, all loci are in accordance with the minimum requirements of HWE and LD. The observed combined probability of discrimination of 8.2201E-28 and the cumulative power of exclusion of 0.9999999995 in Shaanxi Han demonstrated that the studied STR loci are informative and polymorphic, and this system can be used as a powerful routine forensic tool in personal identification and parentage testing. CONCLUSION: Both the geographical and linguistic divisions have shaped the genetic structure of modern East Asian. And more forensic reference data should be obtained for ethnically, culturally, geographically and linguistically different populations for better routine forensic practice and population genetic studies.


Assuntos
Evolução Molecular , Polimorfismo Genético , População/genética , China , Grupos Étnicos/genética , Genética Forense/métodos , Técnicas de Genotipagem/métodos , Humanos , Masculino , Repetições de Microssatélites , Linhagem
16.
Nanotechnology ; 31(27): 275710, 2020 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-32203944

RESUMO

Surface hydroxylation of crude Al2O3 (c-Al2O3) nanoparticles by H2O2 was conducted to tailor the electrical properties of UV-cured resin. The hydroxyl groups on Al2O3 particles were designed to establish hydrogen bonding between the hydroxyl and carboxyl groups, which favors the enhancement of interfacial strength between fillers and UV-cured resin matrix. The effect of interfacial strength on the electrical properties was investigated. Owing to the improved interfacial strength, it can be conjectured that a larger volume of the interaction zone exists in UV-cured resin/hydroxylated Al2O3 (UV/h-Al2O3) composites. As a consequence, the number of deeper traps is increased, restraining the charge migration and raising the charge injection barrier. Thus, UV/h-Al2O3 composites exhibit remarkably enhanced breakdown strength, improved volume resistivity and suppressed space charge accumulation in comparison with that of UV/c-Al2O3 composites at the same filler content. It was found that the addition of 0.5 wt% h-Al2O3 increases the AC breakdown strength and volume resistivity by 15.5% and 367.9%, respectively. Our results suggest that hydroxylation is an efficient way to improve the electrical properties of UV-cured resin nanocomposites, thus promoting stereolithography 3D printing in the application of electrical and electronic fields.

17.
Acta Biochim Biophys Sin (Shanghai) ; 52(2): 168-179, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32047913

RESUMO

Y-box binding protein 1 (YB-1) is manifested as its involvement in cell proliferation and differentiation and malignant cell transformation. Overexpression of YB-1 is associated with glioma progression and patient survival. The aim of this study is to investigate the influence of YB-1 knockdown on glioma cell progression and reveal the mechanisms of YB-1 knockdown on glioma cell growth, migration, and apoptosis. It was found that the knockdown of YB-1 decreased the mRNA and protein levels of YB-1 in U251 glioma cells. The knockdown of YB-1 significantly inhibited cell proliferation, colony formation, and migration in vitro and tumor growth in vivo. Proteome and phosphoproteome data revealed that YB-1 is involved in glioma progression through regulating the expression and phosphorylation of major proteins involved in cell cycle, adhesion, and apoptosis. The main regulated proteins included CCNB1, CCNDBP1, CDK2, CDK3, ADGRG1, CDH-2, MMP14, AIFM1, HO-1, and BAX. Furthermore, it was also found that YB-1 knockdown is associated with the hypo-phosphorylation of ErbB, mTOR, HIF-1, cGMP-PKG, and insulin signaling pathways, and proteoglycans in cancer. Our findings indicated that YB-1 plays a key role in glioma progression in multiple ways, including regulating the expression and phosphorylation of major proteins associated with cell cycle, adhesion, and apoptosis.


Assuntos
Glioma/patologia , Proteína 1 de Ligação a Y-Box/deficiência , Apoptose , Proteínas Reguladoras de Apoptose/análise , Proteínas Reguladoras de Apoptose/metabolismo , Moléculas de Adesão Celular/análise , Moléculas de Adesão Celular/metabolismo , Proteínas de Ciclo Celular/análise , Proteínas de Ciclo Celular/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Técnicas de Silenciamento de Genes , Humanos , Proteínas de Neoplasias/análise , Proteínas de Neoplasias/metabolismo , Fosforilação , Proteômica , RNA Neoplásico/análise , Proteína 1 de Ligação a Y-Box/genética
18.
Waste Manag ; 101: 180-187, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31622863

RESUMO

With the rapid development of photovoltaic industry, the recycling of waste solar photovoltaic (PV) panels is becoming a critical and global challenge. Considering PV panels recycling is significantly effective and worthwhile to save natural resources and reduce the cost of production, how to selectively recycle valuable components of PV panels is the hot and dominant topic. Different from current mechanical crushing, heat treatment and chemical operation processes, novel and environment-friendly recycling approaches by using high voltage pulse discharge in water, called high voltage fragmentation (HVF), was discussed under different discharge conditions. The results showed that discharging across surface and interior of PV panels produced ablation round holes, sputter metal particles and dendritic channels. The average particle size decreased with the ascent of pulse number and voltage amplitude. Considering the energy consumption, the optimal condition of HVF in this paper was 160 kV for 300 pulses with the energy consumption of 192.99 J/g, crushing the PV panels into particles of 4.1 mm in average (13.7% of the initial size). More particle was distributed among the 0.1-2 mm size fractions as the energy increased. Selective fragmented products, such as Cu, Al, Pb, Ag and Sn, are concentrated on the fractions under 1 mm. Finally, hybrid crushing energy consumption model combined with fractal theory was discussed, which presented close relationship between energy and average particle size. Walker's model (n = 2.047 determined by fractal theory) had the best fitting effect.


Assuntos
Resíduo Eletrônico , Metais , Reciclagem
19.
Front Oncol ; 9: 561, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31338322

RESUMO

The aim of this study is to examine the immunocytochemical expression of p53, Ki-67, and CA125 in endometrial brush samples for endometrial cancer. Forty-four patients were recruited with liquid-based cytology preparations during a 5-month period. Both the histological and cytological samples were assessed by histology based on hematoxylin and eosin (H&E), and the expression of p53, CA125, and Ki-67 in endometrial cells was examined by immunocytochemistry. The percentage and intensity of endometrial cells were scored on a scale of 0-3. The final score was calculated by the addition of all partial scores, and then Probit model was used to predict the possibility for malignant lesions. The mean immunoreactivity score of the three immunocytochemical biomarkers (p53, CA125, and Ki-67) in the positive group (including atypical hyperplastic cells and malignant cells) was significantly higher than in the negative group (benign cells and non-atypical hyperplastic cells). The possibility value of the positive group was also significantly higher than the negative group (P < 0.05). The cutoff value of the possibility value was 0.754, the sensitivity and specificity of which were 86.4 and 95.5%. The assessment of p53, CA125, and Ki-67 combined with the prediction model is valuable for the detection of endometrial cancer and atypical hyperplasia in endometrial cytology.

20.
Onco Targets Ther ; 12: 2695-2702, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31114223

RESUMO

Background: The clinicopathological impacts of c-Met overexpression in bladder cancer have been investigated in several studies with conflicting results. We performed this systematic review and meta-analysis to assess the pathologic and prognostic roles of c-Met status in bladder cancer patients. Methods: Eligible studies were searched and identified from the PubMed and China National Knowledge Infrastructure (CNKI) databases (up until October 4, 2018). The DerSimonian-Laird random-effects model was used to calculate the pooled risk estimates. Results: Eight studies including 1,336 bladder cancer cases were eventually included in this meta-analysis. We detected a significantly increased risk of poor overall survival (OS) associated with the high expression of c-Met (HR=2.42, 95% CI 1.36-4.32). There was no association between c-Met status and nuclear grade (OR=0.82, 95% CI 0.29-2.31) or tumor stage (OR=1.42, 95% CI 0.41-4.89). Conclusion: This study shows that the overexpression of c-Met in primary cancer tissues is associated with a worse OS in human bladder cancer. However, larger studies using standardized methods and criteria are warranted to verify these findings.

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