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1.
Molecules ; 24(20)2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31601043

RESUMO

Download ash and emission dust samples were collected from sintering, coking, ironmaking and steelmaking processes of iron and steel enterprises in Laiwu. Sixteen kinds of polycyclic aromatic hydrocarbons (PAHs) in the United States Environmental Protection Agency (USEPA) priority controlled lists were quantitatively analyzed using Gas Chromatography-Mass Spectrometer (GC-MS). Laser particle size analyzer was used to obtain the distribution pattern of download ash. It was found that the diameter distribution pattern from four production processes was quite different. The proportion of fine particulate (0-2.5 µm) was the highest (72.62%) in the steelmaking refining process, and was 28.962% in the ironmaking process. Moreover, the particle size in download ash from steelmaking refining is all less than 10 µm and that from the ironmaking process was 52.92%. The medium-sized particles (10-100 µm) were dominant in sinter and coking download ashes. The total PAHs (∑16PAHs) mass concentration ranged from 0.49 ± 0.06 to 69.63 ± 5.57 µg·g-1 in download ash samples, and varied from 2.815 ± 0.253 to 19.429 ± 2.545 µg·m-3 in emission dust samples. The ∑16PAHs values were both largest in download ash and dust emission from the coking process (69.63 ± 5.57 µg·g-1 and 19.429 ± 2.545 µg·m-3, respectively). The most abundant individual PAHs were benzo[b]fluoranthene, benzo[k]fluoranthene, phenanthrene, benzo[a]anthracene in ash samples, and benzo[a]anthracene, benzo[k]fluoranthene, benzo[b]fluoranthene and indeno[1,2,3-cd]pyrene in emission dust samples. Dominant compounds were high-molecular weight (four- to six-ring) PAHs in both ash and dust samples. The concentration order of individual compounds in PM10 and PM2.5 in ambient air around the steel plant was completely consistent with each other, and the concentration of ∑16PAHs was the highest in the steel plant and lowest in Daqin village because of upwind of the steel plant. The concentrations of benzo[b]fluoranthene and fluoranthene in ambient air were comparatively high, and were in accordance with the higher concentration of the two monomers in the download ash samples, which suggested that the effect of the emission flue gas from the steel plant on ambient air was necessary to concern.

2.
Artif Cells Nanomed Biotechnol ; 47(1): 3438-3447, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31411066

RESUMO

Gliomas are the most common brain tumors in humans. Different chemotherapeutics are available to treat gliomas. However, they are costly and pose numerous side effects. The development of nanocomposite based on chemotherapeutic drug and metallic nanoparticle loaded with polymer could be highly useful against glioma. In this study, carmustine loaded with gold NPs and linked with PLGA-PSPE was produced as a bio-nanocomposite and its efficacy in treating glioma and burn wound were investigated. The synthesized biocomposite was characterized by biophysical techniques. It was observed that the synthesized composite was hexagonal and crystalline nature. TGA analysis showed that the particle had good combustible property. Interestingly, the Cm-Au-PLGA-PSPE composite had exhibited remarkable anti-tumor property against U251 human glioma. The flow cytometry showed a greater increase in the apoptosis rate (62.31%) of glioma cells exposed to the bio-nanocomposite. In addition, a greater reduction in the viability of U251 cells was recorded following treatment with Cm-Au-PLGA-PSPE composite. Quick healing of the heart, liver, spleen, lung and kidney tissue wounds in mouse was noticed with Cm-Au-PLGA-PSPE composite treatment. This study concludes that the newly produced Cm-Au-PLGA-PSPE composite would be a promising alternative in treating human gliomas and associated wounds with increased biomedical applications.

3.
J Photochem Photobiol B ; 197: 111539, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31301638

RESUMO

Treatment of burn injury is clinically challenging one, therefore several steps and noteworthy approaches have been taken to improve wound mechanisms. Citrus pectin plays a stabilizing agent to synthesis of ZnO nanoparticles (ZnO NPs). The present study is focused on ZnO loaded collagen/chitosan nanofibrous were synthesized by electrospinning method using ZnO NPs. The chemical structure, phase purity and morphological observation were investigated under spectroscopic and mircoscopic techniques and demonstrated their suitable properties as a wound healing material. In addition, that prepared nanoparticles loaded biopolymeric fibrous nanomaterial showed suitable antibacterial activity against S. aureus and E. coli bacterial pathogens and also in vitro studies was confirmed the enhanced proliferation, cell viability and biocompatibility. In vitro evaluations have been exhibited acceptable cell proliferation is observed throughout the ZnO loaded Coll/CS nanofibrous within 3 days, which was comparable to the control material. In vivo wound healing ability was monitored on the rat wound experimental model. From the in vivo observations, revealed that the loaded of ZnO NPs with Coll/CS nanofibrous can effectively quicken wound healing mechanism, expressed in the initial stage healing process. These results suggest that ZnO loaded collagen/chitosan nanofibrous is a potential candidate for wound healing applications with enhanced biological properties.


Assuntos
Queimaduras/patologia , Quitosana/química , Colágeno/química , Nanopartículas Metálicas/química , Nanofibras/química , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Bacteriemia/prevenção & controle , Queimaduras/veterinária , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Humanos , Nanofibras/uso terapêutico , Nanofibras/toxicidade , Ratos , Pele/efeitos dos fármacos , Pele/patologia , Staphylococcus aureus/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Óxido de Zinco/química
4.
Medicine (Baltimore) ; 97(20): e10734, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29768346

RESUMO

This study aimed to explore the underlying mechanism of relapsed acute lymphoblastic leukemia (ALL).Datasets of GSE28460 and GSE18497 were downloaded from Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) between diagnostic and relapsed ALL samples were identified using Limma package in R, and a Venn diagram was drawn. Next, functional enrichment analyses of co-regulated DEGs were performed. Based on the String database, protein-protein interaction network and module analyses were also conducted. Moreover, transcription factors and miRNAs targeting co-regulated DEGs were predicted using the WebGestalt online tool.A total of 71 co-regulated DEGs were identified, including 56 co-upregulated genes and 15 co-downregulated genes. Functional enrichment analyses showed that upregulated DEGs were significantly enriched in the cell cycle, and DNA replication, and repair related pathways. POLD1, MCM2, and PLK4 were hub proteins in both protein-protein interaction network and module, and might be potential targets of E2F. Additionally, POLD1 and MCM2 were found to be regulated by miR-520H via E2F1.High expression of POLD1, MCM2, and PLK4 might play positive roles in the recurrence of ALL, and could serve as potential therapeutic targets for the treatment of relapsed ALL.


Assuntos
DNA Polimerase III/genética , MicroRNAs/genética , Componente 2 do Complexo de Manutenção de Minicromossomo/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Proteínas Serina-Treonina Quinases/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Leucemia-Linfoma Linfoblástico de Células Precursoras/fisiopatologia , Recidiva
5.
Medicine (Baltimore) ; 96(18): e6696, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28471964

RESUMO

BACKGROUND: The aim of this meta-analysis was to investigate the prenatal, perinatal, and postnatal risk factors for children autism. METHODS: PubMed, Embase, Web of Science were used to search for studies that examined the prenatal, perinatal, and postnatal risk factors for children autism. A fixed-effects model or random-effects model was used to pool the overall effect estimates. RESULTS: Data from 37,634 autistic children and 12,081,416 nonautistic children enrolled in 17 studies were collated. During the prenatal period, the factors associated with autism risk were maternal and paternal age≥35 years, mother's and father's race: White and Asian, gestational hypertension, gestational diabetes, maternal and paternal education college graduate+, threatened abortion, and antepartum hemorrhage. During perinatal period, the factors associated with autism risk were caesarian delivery, gestational age≤36 weeks, parity≥4, spontaneous labor, induced labor, no labor, breech presentation, preeclampsia, and fetal distress. During the postnatal period, the factors associated with autism risk were low birth weight, postpartum hemorrhage, male gender, and brain anomaly. Parity≥4 and female were associated with a decreased risk of autism. In addition, exposure to cigarette smoking, urinary infection, mother's and father's race: Black and Hispanic, mother's country of birth outside Europe and North America, umbilical cord around neck, premature membrane rupture, 5-minutes Apgar score<7, and respiratory infection were not associated with increased risk of autism. CONCLUSION: The present meta-analysis confirmed the relation between some prenatal, perinatal, and postnatal factors with autism. All these factors were examined individually, thus it was still unclear that whether these factors are causal or play a secondary role in the development of autism. Further studies are needed to verify our findings, and investigate the effects of multiple factors on autism, rather than the single factor.


Assuntos
Transtorno Autístico/epidemiologia , Feminino , Humanos , Gravidez , Complicações na Gravidez/epidemiologia , Fatores de Risco
6.
FEBS Lett ; 591(9): 1295-1304, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28380253

RESUMO

Griseoviridin (GV) is a broad-spectrum antibiotic with antibacterial and antifungal activity. In the GV biosynthetic pathway, SgvP catalyzes formation of the carbon-sulfur bond in GV. Herein, we report the recombinant expression and characterization of SgvP from Streptomyces griseoviridis NRRL2427. We also present the 2.6 Å crystal structure of SgvP, which is the first structure of a cytochrome P450 involved in carbon-sulfur bond formation in GV. Structural analysis indicates that Pro237 in the I-helix of SgvP may play a critical role in dioxygen binding and proton transfer during the catalytic cycle. Of the three channels we observed in SgvP, channel 3 may be essential for substrate ingress and egress from the active site, while channels 1 and 2 may be the solvent and water pathway, respectively. DATABASE: Coordinate and structure factor were deposited in the Protein Data Bank database under the accession number 4MM0.


Assuntos
Proteínas de Bactérias/química , Carbono/química , Peptídeos/química , Enxofre/química , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Biocatálise , Carbono/metabolismo , Domínio Catalítico , Cristalografia por Raios X , Sistema Enzimático do Citocromo P-450/química , Sistema Enzimático do Citocromo P-450/metabolismo , Modelos Moleculares , Estrutura Molecular , Oxigênio/química , Oxigênio/metabolismo , Peptídeos/metabolismo , Prolina/química , Prolina/metabolismo , Conformação Proteica , Homologia de Sequência de Aminoácidos , Espectrofotometria , Streptomyces/genética , Streptomyces/metabolismo , Especificidade por Substrato , Enxofre/metabolismo
7.
Artigo em Chinês | MEDLINE | ID: mdl-26080525

RESUMO

OBJECTIVE: To investigate the application value of empty puparia in species identification of common sarcosaphagous flies. METHODS: Fifty-five samples of adult flies and their empty puparia were collected. All the samples were identified as 2 families, 6 genera and 8 species by morphological characteristics. The samples were divided into 3 groups according to their time period between eclosion and our analyses: less than 2 years (n = 23), 2-5 years (n = 20), and more than 5 years (n = 12). The mtDNA of each sample was extracted by CTAB method. The purity and concentration of DNA were tested. PCR products were amplified using two sets of primers. Two sequences of CO I gene (sequence I: 498 bp, sequence II : 841 bp) from each sample were compared to the sequences in GenBank using BLAST for species identification. RESULTS: The mtDNA was extracted successfully from all the samples. DNA concentration of adult chest muscle preserved less than or equal to 5 years and empty puparia preserved less than 2 years ranged from 1.0 to 3.0 µg/µl, and the value of A260/A280 ranged from 1.6 to 1.8. The purity and concentration was lower than 1.6 and 1.0 µg/µl, when the adult chest muscle and empty puparia preserved more than 5 years and 2 years, respectively. DNA concentration of the samples significantly decreased with the prolonged preservation time (P < 0.01). Two sequences of CO I gene was amplified in adult chest muscle and empty puparia which preserved less than 2 years. The success rates of amplification decreased with the prolonged preservation time, especially for the sequence II (P < 0.01). The morphological identification of 8 species did not match exactly with the results based on the COI gene, correct species identification occurred in 6 and 7 species out of 8 based on the two sequences, respectively, and their Max ident value exceeded 97% CONCLUSION: Empty puparium samples can be used to extract mtDNA and identify species.


Assuntos
Dípteros/classificação , Animais , Primers do DNA , DNA Mitocondrial/genética , Medicina Legal , Reação em Cadeia da Polimerase , Pupa/classificação , Análise de Sequência de DNA , Especificidade da Espécie
8.
Public Health Nutr ; 18(9): 1692-7, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25373938

RESUMO

OBJECTIVE: To explore (i) the prevalence of thyroid dysfunction in populations with adequate and excessive iodine intakes and (ii) the effect of iodine exposure on the prevalence of thyroid dysfunction. DESIGN: Cross-sectional study was conducted in Hebei in 2010. The population was classified as having adequate or excessive iodine intake according to the iodine concentration in drinking water. Demographic information was collected by questionnaire. Levels of serum thyroid hormones, thyroid autoantibodies and iodine in drinking water and urine were measured. SETTING: Villages with adequate or excessive drinking water iodine in Hebei Province, People's Republic of China. SUBJECTS: A total of 854 men and women aged 20-50 years who had lived in the surveyed areas for over 5 years, including 348 from the adequate iodine area (AIA) and 506 from the excessive iodine area (EIA). RESULTS: Median urinary iodine concentration was 185 µg/l in AIA and 1152 µg/l in EIA. The prevalence of thyroid dysfunction in AIA was 10.3%, which included 1.1% with hypothyroidism and 8.1% with subclinical hypothyroidism; and 20.6% in EIA, which included 3.6% with hypothyroidism and 13.6% with subclinical hypothyroidism. The positive rates of thyroglobulin antibody were 16.1% in AIA and 11.9% in EIA; the positive rates of thyroperoxidase antibody were 20.7% in AIA and 16.4% in EIA. CONCLUSIONS: Excessive iodine intake may lead to increased prevalence of biochemical thyroid dysfunction, especially biochemical hypothyroidism. This is not related to an increase in prevalence of thyroid antibodies. Women are more susceptible to iodine excess.


Assuntos
Iodo/administração & dosagem , Iodo/efeitos adversos , Doenças da Glândula Tireoide/epidemiologia , Adulto , China/epidemiologia , Estudos Transversais , Água Potável/análise , Feminino , Humanos , Iodeto Peroxidase/análise , Iodo/urina , Masculino , Pessoa de Meia-Idade , Prevalência , Inquéritos e Questionários , Tireoglobulina/análise , Urinálise
9.
Clin Endocrinol (Oxf) ; 83(3): 399-404, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25280177

RESUMO

OBJECTIVE: The effects of long-term excessive maternal iodine intake on neonatal thyroid function are less known. This study aimed to assess the effects of maternal excessive iodine intake from drinking water on thyroid functions of both mothers and their neonates. DESIGN AND METHODS: This observational study was performed in high iodine (HI) areas and adequate iodine (AI) intake areas, including 384 healthy pregnant women in late gestation (mean week 39·3 ± 1·6 weeks) and their newborns. Blood and urine samples were obtained from pregnant women, while cord blood samples were obtained from neonates. Urinary iodine concentration (UIC) and thyroid function were evaluated. RESULTS: The median maternal UIC was 1241 and 217 µg/l in HI and AI areas, respectively (P < 0·001). The concentrations of serum thyroid-stimulating hormone (TSH), free triiodothyronine (FT3) and free thyroxine (FT4) in neonates in HI areas were 7·33 mIU/l (range 5·47, 11·06 mIU/l), 2·93 ± 0·59 and 15·03 ± 1·92 pmol/l, respectively, while that were 4·71 mIU/l (range 3·96, 6·04 mIU/l), 2·31 ± 0·28 and 16·50 ± 1·35 pmol/l in AI neonates (P < 0·05). Similar changes were also observed in neonates in HI areas when excluding the effect of maternal thyroid autoimmunity. Cord blood TSH concentration (r = 0·31, P = 0·001) and FT3 concentration (r = 0·43, P = 0·001) were positively correlated with maternal UIC. Cord blood FT4 concentration was negatively correlated with maternal UIC (r = -0·25, P = 0·001). Mothers living in HI areas (ß = 0·296, 95% CI: 0·163, 0·255) and with subclinical hypothyroidism (ß = 0·360, 95% CI: 0·034, 0·175) contributed to elevated cord blood TSH concentration in neonates, while male neonates were more likely to present with higher TSH concentration compared with female infants (ß = -0·760, 95% CI: -0·119, -0·033). CONCLUSIONS: Excessive iodine intake during pregnancy was associated with an increased rate of hyperthyrotropinaemia in neonates and their mothers, especially in male neonates.


Assuntos
Água Potável/química , Iodo/efeitos adversos , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Glândula Tireoide/efeitos dos fármacos , Adulto , China , Estudos Transversais , Feminino , Sangue Fetal/química , Geografia , Humanos , Recém-Nascido , Iodo/administração & dosagem , Iodo/urina , Masculino , Gravidez , Efeitos Tardios da Exposição Pré-Natal/sangue , Efeitos Tardios da Exposição Pré-Natal/urina , Testes de Função Tireóidea , Glândula Tireoide/fisiopatologia , Tireotropina/sangue , Tiroxina/sangue , Tri-Iodotironina/sangue , Adulto Jovem
10.
J Nutr ; 143(12): 2038-43, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24108132

RESUMO

Previous studies have indicated an association between iodine excess and increased incidence of thyroid dysfunction in adults. However, there have been few studies on how the intake of excessive iodine affects thyroid function in children. The objective of this study was to assess the effects of a long-term exposure to excessive iodine on thyroid dysfunction in children. Urinary iodine concentration (UIC) and thyroid function in 371 children from a high iodine (HI) area (water iodine: 150-963 µg/L) and 150 children from an adequate iodine (AI) area (water iodine: 12.8-50.9 µg/L) were measured. The water iodine concentration in the HI area was higher than that in the AI area (P < 0.001) and the median urinary iodine concentration of children in the HI area was 1030 µg/L, which was 8.6 times that of children in the AI area (123 µg/L) (P < 0.001). Children in the HI area had a higher concentration of sensitive thyroid stimulating hormone and higher positivity of both thyroid peroxidase antibody (TPOAb) and thyroglobulin antibody (TGAb). The prevalence of thyroid diseases was higher in HI area children than that in AI area children (P = 0.000), especially subclinical hypothyroidism (SCH; P = 0.004). A body mass index (BMI) of ≥ 22.3 kg/m(2) was associated with the incidence of SCH (OR: 5.51; 95% CI: 1.52, 19.9; P = 0.009). UIC ≥ 600 µg/L (OR: 3.62; 95% CI: 1.22, 10.8; P = 0.024) and TPOAb or TGAb-positivity (Ab+ OR: 6.48; 95% CI: 1.78, 23.6; P = 0.005) in children were significantly and independently associated with SCH. Interactions between UIC ≥ 800 µg/L and Ab+ (P-interaction = 0.004) were found. Furthermore, increased thyroid volume was correlated with higher UIC (ß = 0.22; P = 0.002). Excessive iodine intake in children in HI areas is associated with impaired thyroid function; UIC ≥ 600 µg/L and Ab+ are the risk factors for SCH. Effective measures need to be taken for reducing excessive iodine intake.


Assuntos
Iodo/administração & dosagem , Glândula Tireoide/fisiopatologia , Água/química , Criança , Estudos Transversais , Feminino , Humanos , Masculino , Testes de Função Tireóidea
11.
Wei Sheng Yan Jiu ; 42(6): 966-9, 2013 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-24459910

RESUMO

OBJECTIVE: To investigate the iodine nutrition status of the susceptible population after stop supplying iodized salt and discuss whether there is iodine nutritional status difference in different populations in high-iodine areas in China. METHODS: Spot investigation methods were being used, 371 children, 506 adults and 210 cases of pregnant women during the third trimester were selected as respondents. The morning Urine was collected to determinate the urine iodine level. Drinking water was collected to determinate the local water iodine level. RESULTS: The water iodine level was between 150.3-962.6 microg/L in investigation areas; The median urinary iodine (MUI) of children, adults and pregnant woman was respective 1032.08 microg/L, 1152.01 microg/L and 1240.70 microg/L. Meanwhile 96.2% children and 93.1% adults urine iodine level was more than 300 microg/L and 84.3% of pregnant women urine iodine level was more than 500 microg/L, which belongs to excessive iodine intake. The chi-square test on the distribution of urinary iodine indicate that there is statistical differences in three different population (chi2 = 44.84, P = 0.000). Kruskal-Wallis H test show that the MUI in three different crowd are not all the same (chi2 = 12.83, P = 0.002), when compared by pairs, the difference in MUI between pregnant and children or adults was founded. CONCLUSION: The iodine nutrition status of children, adult and the third trimester pregnant women in high water regions were iodine excess. The monitor on iodine nutrition status in different peoples should be enhanced. Urinary iodine level of Children can't completely represent the iodine nutritional status of pregnant women, iodine nutritional status monitor standard for pregnant women should be established.


Assuntos
Iodo/análise , Iodo/urina , Estado Nutricional , Abastecimento de Água/análise , Adolescente , Adulto , Criança , China , Feminino , Humanos , Iodo/administração & dosagem , Masculino , Pessoa de Meia-Idade , Gravidez , Terceiro Trimestre da Gravidez , População Rural , Cloreto de Sódio na Dieta , Adulto Jovem
12.
Wei Sheng Yan Jiu ; 41(4): 536-9, 542, 2012 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-23057312

RESUMO

OBJECTIVE: To investigate the iodine status and the prevalence of thyroid disease and dyslipidemia in adults living in areas of excessive iodine in water in Hebei Province, and to explore the impact of excessive iodine intake on dyslipidemia. METHODS: Subjects were selected from Haixing County in Cangzhou, Hebei. Fasting morning urine and venous blood were collected to test the levels of urinary iodine and serum free triiodothyronine (FT3), free thyroxine (FT4), and sensitive thyroid-stimulating hormone (sTSH). Thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb) and total cholesterol (CHO), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were also measured. RESULTS: The median of urinary iodine was 1094.92 (627.38 - 1511.81) microg/L. There were 66 (22.0%) adults diagnosed as thyroid disorder, including 3 (1.0%) hyperthyroidism patients, 7 (2.3%) subclinical hyperthyroidism patients, 12 (4.0%) hypothyroidism patients and 44 (14.7%) subclinical hypothyroidism patients. The levels of CHO, TG, HDL-C and LDL-C were (5.46 +/- 1.06) mmol/L, 2.19 (1.70 - 2.96) mmol/L, 1.18 (1.03 - 1.45) mmol/L and (3.08 +/- 1.05) mmol/L respectively, no significant difference was observed between the thyroid disorder patients or non-patients. CONCLUSION: The prevalence of dyslipidemia in adults living in areas of excessive iodine in water was high.


Assuntos
Dislipidemias/epidemiologia , Iodo/análise , Adulto , Autoanticorpos , China/epidemiologia , Humanos , Hipotireoidismo , Iodetos , Iodo/metabolismo , Lipídeos/sangue , Prevalência , Doenças da Glândula Tireoide , Testes de Função Tireóidea , Tireotropina , Tiroxina , Tri-Iodotironina , Abastecimento de Água
13.
Zhonghua Yu Fang Yi Xue Za Zhi ; 46(3): 225-7, 2012 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-22800592

RESUMO

OBJECTIVE: To reveal the iodine status of pregnant women and its impact on thyroid function of neonates in high iodine area. METHODS: A total of 210 pregnant women were chosen from a hospital in Haixing county, Hebei province. Pregnant women's random urinary and neonates' cord blood were collected. The urinary iodine concentration (UIC) was examined by arsenic-cerium catalytic spectrophotometry. The levels of free triiodothyronine (FT3), free thyroxine (FT4) and sensitive thyroid-stimulating hormone (sTSH) in serum were measured by chemiluminescence. RESULTS: The age of 210 pregnant women was (27.69 +/- 4.73) years old, whose urinary iodine median (inter-quartile range ) was 1240.70 (1292.68) microg/L. 84.3% (177/210) of the pregnant women had excessive iodine intake (UIC > or = 500 microg/L), 13.8% (29/210) had more than adequate iodine intake( UIC within 250 - 499 microg/L), 0.5% (1/210) had adequate iodine intake (UIC within 150 - 249 microg/L) and 1.4% (3/210) had insufficient iodine intake (UIC < 150 microg/L). The average serum level of FT3, FT4 in neonates were (2.93 +/- 0.59), (15.03 +/- 1.92) pmol/L, respectively. The median (inter-quartile range) of serum sTSH in neonates were 7.33 (5.59) mU/L 78.1% (164/210) of the neonates' serum TSH were beyond 5 mU/L. There were no correlation between pregnant women's urinary iodine level and neonates' serum FT3 and sTSH level (P > 0.05), but there was a positive correlation between pregnant women's urinary iodine level and neonates' serum FT4 level (P < 0.01). Serum FT4 level of the neonates with whose mothers had insufficient iodine intake ((12.99 +/- 1.10) pmol/L) were statistically lower than those with their mothers had excessive iodine intake (15.16 +/- 1.83) pmol/L) (P < 0.05). CONCLUSION: Most of the pregnant women in high iodine area were excessive for iodine nutrition, but still a few pregnant women had insufficient iodine nutrition. The level of neonates' serum sTSH were relatively high and monitoring of risk of hypothyroidism and subclinical hypothyroidism should be strengthened.


Assuntos
Sangue Fetal/química , Iodo/urina , Estado Nutricional , Adulto , China/epidemiologia , Feminino , Humanos , Hipotireoidismo/epidemiologia , Recém-Nascido , Gravidez , Testes de Função Tireóidea , Glândula Tireoide , Tireotropina/sangue , Tiroxina/sangue , Topografia Médica , Tri-Iodotironina/sangue , Adulto Jovem
14.
J Clin Endocrinol Metab ; 97(8): E1363-9, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22669304

RESUMO

CONTEXT: Adequate iodine intake during pregnancy is essential for both the synthesis of maternal thyroid hormones and the maintenance of normal fetal brain development. Scant evidence is available on the effects of excessive iodine intake during pregnancy. OBJECTIVE: The study assesses the relationship between iodine nutritional status and thyroid function of pregnant women with excessive iodine intake during late gestation. DESIGN AND PARTICIPANTS: A cross-sectional study of 384 pregnant women was carried out in Tianjin and Haixing from April to October in 2010. MAIN OUTCOME MEASURES: Morning urine samples and blood samples were obtained from all subjects. Serum levels of free T(3), free T(4), and sensitive TSH and urinary iodine concentration were measured. RESULTS: The median urinary iodine concentration of pregnant women with excessive iodine intake was significantly higher than those with adequate iodine intake (P < 0.001). The prevalence of thyroid disease, especially subclinical hypothyroidism, in pregnant women with excessive iodine intake was significantly higher than in those with adequate iodine intake (P < 0.05). Subclinical hypothyroidism was the most frequent pattern of thyroid disease for pregnant women and those with positive or negative thyroid autoantibodies. Living with high water iodine content and having urinary iodine concentration higher than 250 µg/liter are associated risk factors for subclinical hypothyroidism in pregnant women (OR(1) = 41.822, OR(2) = 6.202; P < 0.05, where OR(1) is the odds ratio for living with high water iodine content and hypothyroidism and OR(2) is the odds ratio for urinary iodine concentration >250 µg/liter and hypothyroidism). CONCLUSIONS: Excessive iodine intake during late pregnancy may lead to maternal thyroid dysfunction, particularly subclinical hypothyroidism. The appropriate measurements should be performed to monitor the onset of hypothyroidism in pregnant women with excessive iodine intake.


Assuntos
Iodo/administração & dosagem , Complicações na Gravidez/etiologia , Doenças da Glândula Tireoide/etiologia , Adulto , Autoanticorpos/sangue , Estudos Transversais , Feminino , Humanos , Iodo/urina , Gravidez , Complicações na Gravidez/fisiopatologia , Doenças da Glândula Tireoide/fisiopatologia , Hormônios Tireóideos/sangue , Tireotropina/sangue
15.
Zhonghua Yu Fang Yi Xue Za Zhi ; 46(2): 148-51, 2012 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-22490198

RESUMO

OBJECTIVE: To understand the level of thyroid function and the prevalence of the thyroid diseases of children in high water iodine areas. METHOD: Two primary schools were selected by purposive sampling from Haixing county, Hebei province in April 2010. A total of 371 children were selected from six classes by cluster sampling in the schools. Morning-urine and venous blood (5 ml) were collected to measure the levels of urinary iodine, free triiodothyronine (FT(3)), free thyroxine (FT(4)) and sensitive thyroid-stimulating hormone (sTSH) in serum and to evaluate the iodine status and thyroid function of children. RESULTS: The median urinary iodine of the children was 1032.08 µg/L. Overall, 96.2% (357/371) of the children's urinary iodine were ≥ 300 µg/L and 68.5% (254/371) were ≥ 800 µg/L. The level of FT(3) and FT(4) were (6.28 ± 0.81) pmol/L and (16.37 ± 2.72) pmol/L, respectively. The median of sTSH was 4.01 mU/L. The FT(4) among boys ((16.63 ± 2.60) pmol/L) was significantly higher than that among girls ((15.99 ± 2.85) pmol/L) (P < 0.05). Overall, 44 subjects were diagnosed as thyroid disease (11.9%), 25 were subclinical hypothyroidism (57% of the total patients), 4 were hypothyroidism (9%), 10 were hyperthyroidism (23%) and 5 were subclinical hyperthyroidism (11%). In the urinary iodine of 200 - 799 µg/L, the prevalence of thyroid disease and hypothyroidism were 6.3% (7/111) and 14.6% (27/254), respectively. When urinary iodine ≥ 800 µg/L, the prevalence of thyroid diseases and hypothyroidism were 3.6% (4/111) and 8.3% (21/254), respectively. CONCLUSION: Most of the children in high water iodine areas had excessive iodine intake. Levels of the children's thyroid hormone were within the normal range. The prevalence of thyroid disease were high, in these areas and the thyroid disease were mainly the subclinical hypothyroidism.


Assuntos
Hipotireoidismo/epidemiologia , Iodo/análise , Água/química , Adolescente , Criança , China/epidemiologia , Feminino , Humanos , Iodo/urina , Masculino , Prevalência , Testes de Função Tireóidea
16.
Free Radic Biol Med ; 52(8): 1343-52, 2012 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-22330063

RESUMO

Excessive oxidative stress has been suggested as one of the underlying mechanisms in the development of thyroid cytotoxicity. Although the involvement of mitochondria has been hypothesized, the effect of early acute high concentrations of iodide on mitochondrial superoxide production remains largely unknown, especially within a 24 h time frame. By using a novel fluorescent probe, MitoSOX Red, we demonstrated the concentration response and time-course response of KI-induced mitochondrial superoxide production in the Fischer rat thyroid cell line (FRTL). A strong increase of MitoSOX Red fluorescence intensity in FRTL cells can be seen at 2 h following high concentrations of iodide exposure. Besides, we indicated that 6-propyl-2-thiouracil (PTU, 300 µM), thyroid-stimulating hormone (TSH, 10 mU/ml), and perchlorate (KClO(4), 30 µM) can inhibit excessive iodide-induced strong mitochondrial superoxide production; however, diethyldithiocarbamic acid (DETC, 2 mM) can further increase excessive iodide-induced mitochondrial superoxide production. By using transmission electron microscopy (TEM), we noted accumulated myelinoid bodies with lipid droplets and numerous apoptotic nuclear bodies at 24 h in FRTL cells. In addition, we demonstrated a significant decrease in cytochrome c (cyt c) content in the mitochondria by enzyme linked immunosorbent assay (ELISA), and DNA fragments and significant increases in lactate dehydrogenase (LDH) activity were detected. We propose a sequence of events mediated by a strong mitochondrial superoxide production at 2 h, followed by lipid peroxidation, cell membrane damage with significant cyt c release, culminating in DNA fragmentation and apoptotic nuclear formation at 24 h, which may partly contribute to the underlying mechanisms of early acute iodide excess.


Assuntos
Iodetos/farmacologia , Mitocôndrias/efeitos dos fármacos , Superóxidos/metabolismo , Animais , Linhagem Celular , Citocromos c/metabolismo , Fragmentação do DNA/efeitos dos fármacos , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo , Corantes Fluorescentes , Peróxido de Hidrogênio/metabolismo , Imuno-Histoquímica , Iodetos/administração & dosagem , Camundongos , Microscopia Eletrônica de Transmissão , Mitocôndrias/enzimologia , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura
17.
Fa Yi Xue Za Zhi ; 24(5): 330-2, 2008 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18979914

RESUMO

OBJECTIVE: To screen the AFLP primers with good diversity to distinguish various species of Cannabis. METHODS: The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations. RESULTS: A total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphic and 10 bands were special, with 47-76 bands amplified in each pair of primers. CONCLUSION: AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.


Assuntos
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos , Cannabis/genética , DNA de Plantas/genética , Variação Genética , Polimorfismo Genético , Cannabis/classificação , DNA de Plantas/análise , Genética Forense
18.
Biochem Biophys Res Commun ; 370(1): 129-33, 2008 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-18358830

RESUMO

Interleukin (IL)-23 plays a predominant role in the development of autoimmune diseases by inducing IL-17-producing helper T (Th17) cells. The receptor for IL-23 consists of a heterodimer composed of the IL-12 receptor beta1 (IL-12Rbeta1) and the IL-23 receptor (IL-23R), which is mainly expressed on Th17 cells. A recent study showed that macrophages express IL-23R mRNA and can be distinguished from microglia by IL-23R expression. However, in this study, we show by RT-PCR and immunocytochemistry that microglia express IL-23R and IL-12Rbeta1 mRNA and protein, respectively. Additionally, microglia expressed a functional receptor for IL-23, as IL-23 enhanced the Interferon (IFN)-gamma-induced signal transducer and activator of transcription (STAT)1 phosphorylation and chemokine production. Thus, IL-23R expression does not discriminate peripheral macrophages from microglia. Moreover, since microglia produce IL-23, it may function in an autocrine manner to recruit inflammatory cells by inducing chemokine production.


Assuntos
Interleucina-23/metabolismo , Microglia/imunologia , Receptores de Interleucina-12/metabolismo , Receptores de Interleucina/metabolismo , Animais , Quimiocinas/genética , Quimiocinas/metabolismo , Interferon gama/farmacologia , Interleucina-23/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Microglia/química , Microglia/efeitos dos fármacos , Fosforilação , RNA Mensageiro/análise , RNA Mensageiro/metabolismo , Receptores de Interleucina/análise , Receptores de Interleucina/genética , Receptores de Interleucina-12/análise , Receptores de Interleucina-12/genética , Fator de Transcrição STAT1/metabolismo
19.
FASEB J ; 22(6): 1797-806, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18198214

RESUMO

Interferon-gamma (IFN-gamma) is a proinflammatory cytokine that plays a pivotal role in pathology of diseases in the central nervous system (CNS), such as multiple sclerosis. However, the direct effect of IFN-gamma on neuronal cells has yet to be elucidated. We show here that IFN-gamma directly induces neuronal dysfunction, which appears as dendritic bead formation in mouse cortical neurons and enhances glutamate neurotoxicity mediated via alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic (AMPA) receptors but not N-methyl-D-aspartate receptors. In the CNS, IFN-gamma receptor forms a unique, neuron-specific, calcium-permeable receptor complex with AMPA receptor subunit GluR1. Through this receptor complex, IFN-gamma phosphorylates GluR1 at serine 845 position by JAK1.2/STAT1 pathway, increases Ca(2+) influx and following nitric oxide production, and subsequently decreases ATP production, leading to the dendritic bead formation. These findings provide novel mechanisms of neuronal excitotoxicity, which may occur in both inflammatory and neurodegenerative diseases in the CNS.


Assuntos
Interferon gama/farmacologia , Complexos Multiproteicos/metabolismo , Neurônios/patologia , Síndromes Neurotóxicas/etiologia , Receptores de AMPA/metabolismo , Receptores de Interferon/metabolismo , Trifosfato de Adenosina/biossíntese , Animais , Cálcio/metabolismo , Células Cultivadas , Córtex Cerebral/citologia , Camundongos , Fosforilação
20.
Ann N Y Acad Sci ; 1088: 219-29, 2006 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17192568

RESUMO

Accumulation of activated microglia and reactive astrocytes is observed around degenerating neurons in various inflammatory or degenerative disorders in the central nervous system. These reactive glial cells may play either neurotoxic or neuroprotective roles. In this study, we examined the effects of glia-derived cytokines on neuronal degeneration and regeneration. Neuron-rich cultures were stimulated with supernatant of microglia and astrocytes stimulated with LPS, or a various concentrations of recombinant cytokines. Neurotoxicity was evaluated by an MTS assay. Neuronal damage was also evaluated by a frequency of dendritic beading, which was found to be an early feature of neuronal damage toward cell death. Effects of the cytokines on production of neurotrophic factors by astrocytes were also examined by RT-PCR for the expression of mRNA. Supernatant of LPS-stimulated microglia induced neuronal cell death. However, all the recombinant cytokines examined did not induce cell death, while IFNgamma and TNFalpha induced dendrite beading, an early feature of neuronal damage. IL-1beta and TNFalpha enhanced the production of neurotrophic factors by astrocytes. These observations suggest that glial cell-derived cytokines may synergistically function in neuronal degeneration with other toxic factors produced by activated microglia, and that some of them may also function in regeneration by inducing neurotrophic factors.


Assuntos
Citocinas/imunologia , Microglia/imunologia , Degeneração Neural/imunologia , Regeneração Nervosa/imunologia , Neuroimunomodulação/imunologia , Animais , Humanos
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