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J Biosci Bioeng ; 111(1): 50-4, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20801714

RESUMO

Response surface methodology (RSM) was used to optimize the extraction parameters for Pholiota adiposa SX-02 intracellular polysaccharide (IPS) produced during submerged culture. The optimum conditions of IPS extraction were predicted to be, ultrasonic power at 564.93 W, precipitation time 30.34 h and pH 8.28, and IPS yield was estimated at 19.75%. The actual value of IPS under these conditions was 20.51%. The in vitro antioxidant results showed that the inhibition effects of IPS at a dosage of 250 mg/l on superoxide anion, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were 74.66 ± 5.31%, 69.20 ± 5.13%, and 75.20 ± 6.73%, respectively, which were 27.51 ± 2.23%, 16.58 ± 1.33%, and 9.46 ± 0.72% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.32 ± 0.02 (absorbance at 700 nm), 39.13 ± 3.47% higher than that of BHT. The results provide a reference for large-scale extraction of IPS by P. adiposa SX-02 in industrial fermentation and the IPS can be used as a potential antioxidant which enhances adaptive immune responses.


Assuntos
Depuradores de Radicais Livres/isolamento & purificação , Pholiota/metabolismo , Polissacarídeos/isolamento & purificação , Compostos de Bifenilo/metabolismo , Meios de Cultura , Depuradores de Radicais Livres/farmacologia , Concentração de Íons de Hidrogênio , Radical Hidroxila/metabolismo , Picratos/metabolismo , Polissacarídeos/farmacologia
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