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1.
Artigo em Inglês | MEDLINE | ID: mdl-34639810

RESUMO

Exploring and analyzing the common demands and behavioral responses of different stakeholders is important for revealing the mediating mechanisms of ecosystem service (ES) and realizing the management and sustainable supply of ES. This study took Mizhi County, a poverty-stricken area on the Loess Plateau in China, as an example. First, the main stakeholders, common demands, and behavioral responses in the food provision services were identified. Second, the relationship among stakeholders was analyzed. Finally, this study summarized three types of mediating mechanisms of food provision services and analyzed the influence of the different types of mediating mechanisms. The main conclusions are as follows: (1) Five main stakeholders in the study area were identified: government, farmers, enterprises, cooperatives, and middlemen. (2) Increasing farmers' income is the common demand of most stakeholders in the study area, and this common demand has different effects on the behavioral responses of different stakeholders. (3) There are three types of mediating mechanisms in the study area: government + farmers mediating corn and mutton, government + enterprises mediating millet, and government + cooperatives mediating apples. On this basis, the effects of the different types of mediating mechanisms on variations in food yield, and trade-offs and synergies in typical townships, were analyzed.

2.
Adv Mater ; : e2104562, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34595770

RESUMO

The redox-targeting (RT) process or redox-mediated process, which provides great operation flexibility in circumventing the constraints intrinsically posed by the conventional electrochemical systems, is intriguing for various energy storage and conversion applications. Implementation of the RT reactions in redox-flow cells, which involves a close-loop electrochemical-chemical cycle between an electrolyte-borne redox mediator and an energy storage or conversion material, not only boosts the energy density of flow battery system, but also offers a versatile research platform applied to a wide variety of chemistries for different applications. Here, the recent progress of RT-based energy storage and conversion systems is summarized and great versatility of RT processes for various energy-related applications is demonstrated, particularly for large-scale energy storage, spatially decoupled water electrolysis, electrolytic N2 reduction, thermal-to-electrical conversion, spent battery material recycling, and more. The working principle, materials aspects, and factors dictating the operation are highlighted to reveal the critical roles of RT reactions for each application. In addition, the challenges lying ahead for deployment are stated and recommendations for addressing these constraints are provided. It is anticipated that the RT concept of energy materials will provide important implications and eventually offer a credible solution for advanced large-scale energy storage and conversion.

3.
Psychol Rep ; : 332941211043458, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34596458

RESUMO

While there has been research focused on interpersonal relationships and their impact on stress and well-being, no instrument has been developed to comprehensively evaluate interpersonal stressors. This research sought to develop and validate an Interpersonal Stressors Scale (ISS) for Chinese college students through three studies. Focus groups were used to generate the initial item pool (Study 1). Then two large samples (N1 = 511; N2 = 330) were collected to explore the factor structure of the ISS and subsequently examine its reliability and validity estimates (study 2 and 3). Initial results indicated a model with 27 items and five first-order factors (interaction difficulty, behaving as expected, social criticism, relationship maintenance, and indebtedness avoidance) as well as two second-order factors (self-imposed stressors and other-imposed stressors) with strong psychometric properties. Criterion-related validity estimates indicated these two kinds of stressors were both associated with stress while having different relationships with general anxiety, depression, social anxiety, interpersonal satisfaction, and self-efficacy in social interactions. The nature and function of the structure for the ISS were discussed as well as the practical and research implications.

4.
Neural Plast ; 2021: 4430594, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34616448

RESUMO

Background: In recent years, a growing number of researchers showed significant interest in psychological and social interventions to manage chronic musculoskeletal (MSK) pain. Cognitive and emotional empathy is an attractive and valuable sociopsychological factor that may provide protection and resilience against chronic MSK pain. However, its effect on outpatients remains underexplored. Objective: To compare the empathy ability between chronic MSK pain outpatients and healthy controls and explore the relationship between cognitive/emotional empathy and chronic pain. Methods: Patients with chronic MSK pain (n = 22) and healthy controls (n = 26) completed the pain assessment and empathy ability task, utilizing a multidimensional empathy assessment tool with satisfactory reliability and validity (i.e., the Chinese version of the Multifaceted Empathy Test (MET-C)). Results: The data indicated that the chronic MSK pain outpatients had impaired cognitive empathy (i.e., lower squared cognitive empathy accuracy: Student's t = -2.119, P = 0.040, and longer task completion time: Student's t = 3.382, P = 0.002) compared to healthy controls, and cognitive empathy was negatively correlated with pain intensity (r = -0.614, P = 0.002). Further, the impaired cognitive empathy was present in identifying positive, but not negative emotions. Conclusion: These results indicate that chronic MSK pain is associated with impaired empathy ability. Our studies contribute to offering a potential direction for developing psychosocial interventions to treat chronic MSK pain.

5.
Dev Comp Immunol ; 127: 104296, 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34656644

RESUMO

Stimulator of interferon gene (STING), an adaptor molecule in the immune system, is involved in mediating the response to viral and bacterial infections, anti-tumor immunity, autoimmune diseases, and lipid metabolism. There have been reports on the cloning and function of STING in humans, pigs, chickens, and cats; however, STING has not been characterized in non-human primates or monkeys to date. Therefore, in this study, the rhesus macaque (Macaca mulata) STING gene was cloned, and we performed preliminary functional tests to examine its role in the interferon (IFN) pathway. The M. mulatta STING complementary DNA was 1140 bp in length and encoded 380 amino acid residues. Phylogenetic analysis showed that Homo sapiens and M. mulatta STING are closely related and clustered on the same branch. M. mulatta STING was confirmed to increase the promoter activities of IFN-ß, nuclear factor-κB, and interferon-sensitive response element, and STING overexpression increased the mRNA levels of IFN-α, IFN-ß, and interferon regulatory factor 3. Infection of Marc-145 cells with porcine reproductive and respiratory syndrome virus activated STING, and its expression increased along with increases in viral multiplicity of infection titer and time. Moreover, STING expression was time- and dose-dependently up-regulated by poly (I:C) and poly (dA:dT) treatments in Marc-145 cells. In summary, these results highlight STING as a vital immune system signal protein in the IFN pathway. This study provides a basis for understanding the immune characteristics of M. mulatta, and may have important implications for both monkey and human diseases.

6.
Neuroimage Clin ; 32: 102854, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34666289

RESUMO

Accurate detection and segmentation of multiple sclerosis (MS) brain lesions on magnetic resonance images are important for disease diagnosis and treatment. This is a challenging task as lesions vary greatly in size, shape, location, and image contrast. The objective of our study was to develop an algorithm based on deep convolutional neural network integrated with anatomic information and lesion-wise loss function (ALL-Net) for fast and accurate automated segmentation of MS lesions. Distance transformation mapping was used to construct a convolutional module that encoded lesion-specific anatomical information. To overcome the lesion size imbalance during network training and improve the detection of small lesions, a lesion-wise loss function was developed in which individual lesions were modeled as spheres of equal size. On the ISBI-2015 longitudinal MS lesion segmentation challenge dataset (19 subjects in total), ALL-Net achieved an overall score of 93.32 and was amongst the top performing methods. On the larger Cornell MS dataset (176 subjects in total), ALL-Net significantly improved both voxel-wise metrics (Dice improvement of 3.9% to 35.3% with p-values ranging from p < 0.01 to p < 0.0001, and AUC of voxel-wise precision-recall curve improvement of 2.1% to 29.8%) and lesion-wise metrics (lesion-wise F1 score improvement of 12.6% to 29.8% with all p-values p < 0.0001, and AUC of lesion-wise ROC curve improvement of 1.4% to 20.0%) compared to leading publicly available MS lesion segmentation tools.

7.
Nanoscale ; 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34668498

RESUMO

Transition-metal selenides are gaining prominence as promising electrode materials for energy storage applications owing to their low electronegativity and environment-friendliness compared with metal sulfides/oxides. Herein, a CuCoSe@NC nanocomposite with copper-cobalt selenides embedded in highly graphitized N-doped porous carbon was synthesized by an in situ encapsulation strategy with metal-organic framework crystals (CuCo-BDC) as templates followed by selenization, and used as a bifunctional electrocatalyst for Zn-air batteries in lye. The result shows that the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) catalytic activity of the optimal CuCoSe@NC-2 was enhanced, and the assembled Zn-air batteries exhibited a remarkable electrochemical performance with the use of the CuCoSe@NC-2 electrode, including a high power density (137.1 mW cm-2) and excellent charge-discharge cycling stability, which were better than those of the Pt/C + RuO2 electrocatalyst. Such improvement is attributed not only to the higher porosity and larger specific surface area (342 m2 g-1) of the carbon matrix, which increased the contact area with oxygen-containing species, but also the encapsulation effect of the highly graphitized N-doped carbon layer and the high content of pyridine-N species also further improved the conductivity of selenide composites. This work has introduced N-doped bimetallic selenides as an ideal candidate for bifunctional electrocatalysts.

8.
Chin Med J (Engl) ; 134(19): 2316-2321, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34629417

RESUMO

BACKGROUND: Multiple techniques are commonly used for posterior cruciate ligament (PCL) reconstruction. However, the optimum method regarding the fixation of PCL reconstruction after PCL tears remains debatable. The purpose of this study was to compare the biomechanical properties among three different tibial fixation procedures for transtibial single-bundle PCL reconstruction. METHODS: Thirty-six porcine tibias and porcine extensor tendons were randomized into three fixation study groups: the interference screw fixation (IS) group, the transtibial tubercle fixation (TTF) group, and TTF + IS group (n = 12 in each group). The structural properties of the three fixation groups were tested under cyclic loading and load-to-failure. The slippage after the cyclic loading test and the stiffness and ultimate failure load after load-to-failure testing were recorded. RESULTS: After 1000 cycles of cyclic testing, no significant difference was observed in graft slippage among the three groups. For load-to-failure testing, the TTF + IS group showed a higher ultimate failure load than the TTF group and the IS group (876.34 ±â€Š58.78 N vs. 660.92 ±â€Š77.74 N [P < 0.001] vs. 556.49 ±â€Š65.33 N [P < 0.001]). The stiffness in the TTF group was significantly lower than that in the IS group and the TTF + IS group (92.77 ±â€Š20.16 N/mm in the TTF group vs. 120.27 ±â€Š15.66 N/m in the IS group [P = 0.001] and 131.79 ±â€Š17.95 N/mm in the TTF + IS group [P < 0.001]). No significant difference in the mean stiffness was found between the IS group and the TTF + IS group (P = 0.127). CONCLUSIONS: In this biomechanical study, supplementary fixation with transtibial tubercle sutures increased the ultimate failure load during load-to-failure testing for PCL reconstruction.


Assuntos
Reconstrução do Ligamento Cruzado Posterior , Animais , Fenômenos Biomecânicos , Suturas , Suínos , Tendões/cirurgia , Tíbia/cirurgia
9.
Zhongguo Zhong Yao Za Zhi ; 46(16): 4131-4138, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34467724

RESUMO

Eleven condensed tannins were isolated from the roots of Indigofera stachyodes by various column chromatography techniques including silica gel, octadecyl silica(ODS), Sephadex LH-20, and semi-preparative high performance liquid chromatography(HPLC). These compounds were identified on the basis of physicochemical properties, nuclear magnetic resonance(NMR) and mass spectrometry(MS) data as stachyotannin A(1), epicatechin-(2ß→O→7,4ß→8)-epiafzelechin-(4ß→8)-catechin(2), cinnamtannin D1(3), cinnamtannin B1(4), epicatechin-(2ß→O→7,4ß→8)-epiafzelechin-(4α→8)-epicatechin(5), gambiriin C(6), proanthocyanidin A1(7), proanthocyanidin A2(8), aesculitannin B(9), proanthocyanidin A4(10), and procyanidin B5(11). Compound 1 is a new compound. Compounds 2-11 were isolated from Indigofera for the first time. Furthermore, compounds 1, 2, and 4-11 showed inhibitory effects on thrombin-induced ATP release in platelets.


Assuntos
Indigofera , Proantocianidinas , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética , Extratos Vegetais
10.
Proc Inst Mech Eng H ; : 9544119211041186, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34479452

RESUMO

Beta-tricalcium phosphate (ß-TCP) refers to one ideal bone repair substance with good biocompatibility and osteogenicity. A digital light processing (DLP)-system used in this study creates bioceramic green part by stacking up layers of photocurable tricalcium phosphate-filled slurry with various ß-TCP weight fractions. Results show that the sintering shrinkage is anisotropic and the shrinkage vertically reaches over that horizontally. The obtained porous ß-TCP parts have both macroporous outer structure and microporous inner structure, the macropore size is 400-600 µm and the micropore size is 500-1500 nm. The mechanical tests show that the porous ß-TCP bioceramic's compressive strength reaches 16.53 MPa. The cell culture confirmed that the porous ß-TCP bioceramic is capable of achieving the effective attaching, growing, and proliferating pertained to mouse osteoblast cells. This study identified considerable blood vessels and significant ectopic bone forming obviously based on the histologically-related assessment when implanting to rabbit femoral condyle deficiency for 3 months. Thus, under high bioactive property and osteoinductivity, and large precision and mechanical strength that can be adjusted, the DLP printed porous ß-TCP ceramics is capable of being promising for special uses of bones repairing.

11.
Adv Mater ; : e2007778, 2021 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-34510563

RESUMO

Cancer has been one of the most common life-threatening diseases for a long time. Traditional cancer therapies such as surgery, chemotherapy (CT), and radiotherapy (RT) have limited effects due to drug resistance, unsatisfactory treatment efficiency, and side effects. In recent years, photodynamic therapy (PDT), photothermal therapy (PTT), and chemodynamic therapy (CDT) have been utilized for cancer treatment owing to their high selectivity, minor resistance, and minimal toxicity. Accumulating evidence has demonstrated that selective delivery of drugs to specific subcellular organelles can significantly enhance the efficiency of cancer therapy. Mitochondria-targeting therapeutic strategies are promising for cancer therapy, which is attributed to the essential role of mitochondria in the regulation of cancer cell apoptosis, metabolism, and more vulnerable to hyperthermia and oxidative damage. Herein, the rational design, functionalization, and applications of diverse mitochondria-targeting units, involving organic phosphine/sulfur salts, quaternary ammonium (QA) salts, peptides, transition-metal complexes, guanidinium or bisguanidinium, as well as mitochondria-targeting cancer therapies including PDT, PTT, CDT, and others are summarized. This review aims to furnish researchers with deep insights and hints in the design and applications of novel mitochondria-targeting agents for cancer therapy.

12.
Adv Mater ; : e2106175, 2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34561930

RESUMO

Liquid crystal elastomers (LCEs) are a class of soft active materials of increasing interest, because of their excellent actuation and optical performances. While LCEs show biomimetic mechanical properties (e.g., elastic modulus and strength) that can be matched with those of soft biological tissues, their biointegrated applications have been rarely explored, in part, due to their high actuation temperatures (typically above 60 °C) and low biaxial actuation performances (e.g., actuation strain typically below 10%). Here, unique mechanics-guided designs and fabrication schemes of LCE metamaterials are developed that allow access to unprecedented biaxial actuation strain (-53%) and biaxial coefficient of thermal expansion (-33 125 ppm K-1 ), significantly surpassing those (e.g., -20% and -5950 ppm K-1 ) reported previously. A low-temperature synthesis method with use of optimized composition ratios enables LCE metamaterials to offer reasonably high actuation stresses/strains at a substantially reduced actuation temperature (46 °C). Such biocompatible LCE metamaterials are integrated with medical dressing to develop a breathable, shrinkable, hemostatic patch as a means of noninvasive treatment. In vivo animal experiments of skin repair with both round and cross-shaped wounds demonstrate advantages of the hemostatic patch over conventional strategies (e.g., medical dressing and suturing) in accelerating skin regeneration, while avoiding scar and keloid generation.

13.
Cell Rep ; 36(11): 109708, 2021 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-34506741

RESUMO

Cellular immunity is important in determining the disease severity of COVID-19 patients. However, current understanding of SARS-CoV-2 epitopes mediating cellular immunity is limited. Here we apply T-Scan, a recently developed method, to identify CD8+ T cell epitopes from COVID-19 patients of four major HLA-A alleles. Several identified epitopes are conserved across human coronaviruses, which might mediate pre-existing cellular immunity to SARS-CoV-2. In addition, we identify and validate four epitopes that were mutated in the newly circulating variants, including the Delta variant. The mutations significantly reduce T cell responses to the epitope peptides in convalescent and vaccinated samples. We further determine the crystal structure of HLA-A∗02:01/HLA-A∗24:02 in complex with the epitope KIA_S/NYN_S, respectively, which reveals the importance of K417 and L452 of the spike protein for binding to HLA. Our data suggest that evading cellular immunity might contribute to the increased transmissibility and disease severity associated with the new SARS-CoV-2 variants.


Assuntos
Linfócitos T CD8-Positivos/imunologia , COVID-19/imunologia , Epitopos de Linfócito T/imunologia , Imunidade Celular/imunologia , SARS-CoV-2/imunologia , Sequência de Aminoácidos , Humanos , Glicoproteína da Espícula de Coronavírus/imunologia
14.
Chem Commun (Camb) ; 57(77): 9894-9897, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34494043

RESUMO

Herein, we report the first example of controllable magnetoresistance in a semiconducting carbonized phthalonitrile resin. This special phenomenon is explained using the different ratios of graphite-like (sp2) and diamond-like (sp3) bonds and localization length (a0) as well as the density of states at the Fermi-level (N(EF)).

15.
Bioorg Chem ; 116: 105273, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34474304

RESUMO

The ubiquitin-specific protease 7 (USP7)-murine double minute 2 (MDM2)-p53 network plays an important role in the regulation of p53, a tumor suppressor which plays critical roles in regulating cell growth, proliferation, cell cycle progression, apoptosis and immune response. The overexpression of USP7 and MDM2 in human cancers contributes to cancer initiation and progression, and their inhibition reactivates p53 signalings and causes cell cycle arrest and apoptosis. Herein, the current state of pharmacological characterization, potential applications in cancer treatment and mechanism of action of small molecules used to target and inhibit MDM2 and USP7 proteins are highlighted, along with the outcomes in clinical and preclinical settings. Moreover, challenges and advantages of these strategies, as well as perspectives in USP7-MDM2-p53 field are analyzed in detail. The investigation and application of MDM2 and USP7 inhibitors will deepen our understanding of the function of USP7-MDM2-p53 network, and feed in the development of effective and safe cancer therapies where USP7-MDM2-p53 network is implicated.

16.
J Colloid Interface Sci ; 606(Pt 1): 167-176, 2021 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-34388569

RESUMO

Layered metal sulfides are considered as promising candidates for potassium ion batteries (KIBs) owing to the unique interlayer passages for ion diffusion. However, the insufficient electronic conductivity, inevitable volume expansion, and sulfur loss hinder the promotion of K-ion storage performance. Herein, few-layered Ti3C2Tx nanosheets were selected as the multi-functional substrate for cooperating few-layered SnS2 nanosheets, constructing SnS2/Ti3C2Tx hetero-structural nanosheets (HNs) with the thickness as thin as about 5 nm. In this configuration, the formed Ti-S bonds provide robust interaction between SnS2 and Ti3C2Tx nanosheets, which hinders the agglomeration of SnS2 and the restack of Ti3C2Tx, endowing the hybrid material with robust nanostructure. Thus, the shortcomings of the SnS2 anode are muchly relieved. In this way, the as-prepared SnS2/Ti3C2Tx HNs electrode delivers reversible capacities of 462.1 mAh g-1 at 0.1 A g-1 and 166.1 mAh g-1 at 2.0 A g-1, respectively, and a capacity of 85.5 mAh g-1 is remained even after 460 cycles at 2.0 A g-1. These results are superior to those of the counterpart electrode, confirming aggressive promotion of K-ion storage performance of SnS2 anode brought by the cooperation of Ti3C2Tx, and presenting a reliable strategy to improve the electrochemical performance of sulfide anodes.

17.
BMC Psychiatry ; 21(1): 395, 2021 08 09.
Artigo em Inglês | MEDLINE | ID: mdl-34372807

RESUMO

BACKGROUND: The Coronavirus Disease 2019 (COVID-19) pandemic continues to threaten the physical and mental health of people across the world. This study aimed to understand the psychological impact of this disease on adolescents with major depressive disorder (MDD) at 1 month after the start of the outbreak in China. METHODS: Using the Children's Impact of Event Scale (CRIES-13) questionnaire, we investigated the occurrence of posttraumatic stress disorder (PTSD) in two groups of adolescents: MDD patients who were in continuous antidepressant therapy and healthy controls. Total scores and factor subscores were compared between the two groups and subgroups stratified by sex and school grade. Logistic regression was used to identify variables associated with high total CRIES-13 scores. RESULTS: Compared to controls (n = 107), the MDD group (n = 90) had higher total CRIES-13 scores and a higher proportion with a total score ≥ 30. They also had a lower intrusion subscore and a higher arousal subscore. In the MDD group, males and females did not differ significantly in total CRIES-13 scores or factor subscores, but junior high school students had higher avoidance subscores than senior high school students. Logistic regression showed high total CRIES-13 scores to be associated with MDD and the experience of "flashbacks" or avoidance of traumatic memories associated with COVID-19. CONCLUSIONS: It is crucial to understand the psychological impact of COVID-19 on adolescents with MDD in China, especially females and junior high school students. Long-term monitoring of adolescents with a history of mental illness is required to further understand these impacts. TRIAL REGISTRATION: ChiCTR, ChiCTR2000033402 , Registered 31 May 2020.


Assuntos
COVID-19 , Transtorno Depressivo Maior , Transtornos de Estresse Pós-Traumáticos , Adolescente , Criança , China/epidemiologia , Estudos Transversais , Depressão , Transtorno Depressivo Maior/epidemiologia , Surtos de Doenças , Feminino , Humanos , Masculino , SARS-CoV-2 , Transtornos de Estresse Pós-Traumáticos/epidemiologia
19.
Reprod Sci ; 2021 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-34406638

RESUMO

In the past 5 years, the time-lapse culture system (TLS) has gradually been applied to the field of assisted reproduction. However, there are few reports on the comparison between this system and the conventional culture system (CS) on the outcome of newborns. As a new model in embryo culture, we should pay attention to its neonatal outcomes. We examined 15,252 fresh transfer cycles processed between January 2016 and December 2019. After propensity score matching, embryos in two groups, TLS and CS, had similar rates of miscarriages, ectopic pregnancy, and live delivery (P > 0.05). Embryos in the TLS group achieved higher clinical pregnancy and implantation rates (P < 0.05). Of the perinatal and neonatal outcomes, singletons and twins were analyzed separately. No significant differences were observed in gestational age, preterm deliveries, birth weight, and sex ratio (P > 0.05). Incidences of malformations of babies delivered from CS and TLS groups were also similar between the two groups. Embryos cultured in a TLS are as safe as CS with regard to neonatal outcomes, including congenital malformations.

20.
Int J Biol Macromol ; 188: 72-81, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34364938

RESUMO

The extrusion 3D printing of hydrogels has evolved as a promising approach that can be applied for specific tissue repair. However, the printing process of hydrogel scaffolds with high shape fidelity is inseparable from the complex crosslinking strategy, which significantly increases the difficulty and complexity of printing. The aim of this study was to develop a printable hydrogel that can extrude at room temperature and print scaffolds with high shape fidelity without any auxiliary crosslinking during the printing process. To this end, a novel formulation consisting of a Laponite suspension with a high solid concentration and a gelatine methacrylate (GelMA) nanocomposite hydrogel was developed. A homogeneously dispersed high-concentration (up to 20% w/v) Laponite suspension was obtained by stirring at 0 °C. The addition of Laponite with high concentration improved the rheological properties, the degradation stability, and the mechanical strength of the hydrogel. The formulation of 15% (w/v) GelMA and 8% (w/v) Laponite nanocomposite hydrogel exhibited desirable printability and biocompatibility. The GelMA/Laponite hydrogels significantly promoted bone marrow mesenchymal stem cell (BMSC) proliferation and osteogenic differentiation. Both desirable printability under mild conditions and cyto-compatibility enable composite hydrogel a potential candidate as biomaterial inks to be applied for bone tissue regeneration.

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