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1.
Food Chem ; 367: 130659, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34343800

RESUMO

This study presents a sensitive approach for electrochemical determination of 5-hydroxymethylfurfural (5-HMF) in food. The electrochemical sensor was fabricated on a copper electrode (CuE) modified with co-electrodeposited Cu-Ni bimetallic particles. This sensor, fabricated by 30 cycles of cyclic voltametric scanning with a scan rate of 50 mV s-1, exhibits good electrocatalytic ability to 5-HMF oxidation. Under the optimal conditions, linear scan voltammetry (LSV) and chronoamperometry were conducted for the determination of 5-HMF. The results of LSV show that a linear dependency within the 0.4-10 mM range with a detection limit (LOD) of 3.51 µM (S/N = 3) was achieved, while a linear range in 1 × 10-4-11 mM with a LOD of 0.043 µM (S/N = 3) was obtained by chronoamperometric measurement. The electrochemical sensor was finally applied in determination of 5-HMF in various foods, and the reliability and accuracy of the method were assessed by adopting an UV method as a standard method. Results show that the concentrations of 5-HMF in real samples are close to those measured by the standard method. In addition, standard addition method was further performed to evaluate the accuracy of our approach. The recoveries ranged from 90.0% to 110.0% are calculated, demonstrating good accuracy of the electrochemical sensor.


Assuntos
Cobre , Técnicas Eletroquímicas , Eletrodos , Furaldeído/análogos & derivados , Limite de Detecção , Reprodutibilidade dos Testes
2.
Opt Lett ; 46(19): 4781-4784, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34598198

RESUMO

We report an omnidirectional light absorption enhancement of a perovskite solar cell (PSC) using antireflection (AR) film with soft imprinted microstructures from master molds via holographic lithography technology, which has high throughput and repeatability. The PSC's omnidirectional power conversion efficiency (PCE) enhancement is achieved by reducing Fresnel surface reflections and enhancing the optical path length. The maximum PCE of PSCs with AR film is up to 20.27%, corresponding to an absolute increase of 0.93% compared to 19.34% of control devices. Significantly, the enhancements of PCE increase with incident angle enlargement, which attributes to more effective Fresnel surface reflection suppression. Moreover, AR films exhibit water and dust repellent properties due to hydrophobicity, which is beneficial for PSC's long-term stability and light harvesting.

3.
Medicine (Baltimore) ; 100(35): e26544, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34477114

RESUMO

BACKGROUND: To lower albuminuria and to achieve blood pressure (BP) goals, dual renin-angiotensin-aldosterone system (RAAS) inhibitors are sometimes used in clinical practice for the treatment of CKD. However, the efficacy and safety of dual RAAS blockade therapy remains controversial. METHODS: PubMed, EMBASE, and Cochrane Library were searched, and random effects model was used to calculate the effect sizes of eligible studies. Potential sources of heterogeneity were detected by meta-regression and subgroup analysis. RESULTS: The present meta-analysis of 72 randomized controlled trials with 10,296 patients demonstrated that dual RAAS blockade therapy was superior to monotherapy in reducing the urine albumin excretion, urine protein excretion, and BP. These beneficial effects were related to the decrease of glomerular filtration rate, the increase of serum potassium level, and higher rates of hyperkalemia and hypotension. Meanwhile, these effects did not lead to improvements in short-term or long-term outcomes, including doubling of serum creatinine, acute kidney injury, end-stage renal disease, mortality, and hospitalization. Compared with the single therapy, angiotensin-converting enzyme inhibitor (ACEI) in combination with angiotensin-receptor blocker (ARB) was a better dual therapy than ACEI or ARB in combination with renin inhibitor or aldosterone receptor antagonist in decreasing urine albumin excretion, urine protein excretion and BP, and the combination was not associated with a lower glomerular filtration rate. CONCLUSION: Compared with the single therapy, ACEI in combination with ARB was a better dual therapy than ACEI or ARB in combination with renin inhibitor or aldosterone receptor antagonist. Although ACEI in combination with ARB was associated with higher incidences of hyperkalemia and hypotension, careful individualized management and potassium binders may further expand its application (PROSPERO number CRD42020179398).


Assuntos
Antagonistas de Receptores de Angiotensina/uso terapêutico , Insuficiência Renal Crônica/tratamento farmacológico , Sistema Renina-Angiotensina/efeitos dos fármacos , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto/estatística & dados numéricos , Insuficiência Renal Crônica/fisiopatologia
4.
Aging (Albany NY) ; 13(17): 21642-21658, 2021 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-34516406

RESUMO

In this study, we investigated the mechanisms through which astrocyte-derived exosomes (AS-Exos) alleviate traumatic brain injury (TBI)-induced neuronal defects in TBI model rats and mice. Treatment with AS-Exos alleviated neurobehavioral deficits, cognitive impairment, and brain edema in TBI rats. AS-Exos also significantly reduced neuronal cell loss and atrophy in the TBI rats. AS-Exos significantly reduced oxidative stress and mitochondrial H2O2 levels by increasing the activity of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) in the hippocampal neurons of TBI rats. TUNEL-staining assays showed that AS-Exos significantly reduced TBI-induced neuronal apoptosis. Mechanistically, AS-Exos ameliorated oxidative stress by activating Nrf2/HO-1 signaling in the hippocampus of TBI rats. In addition, the neuroprotective effects of AS-Exos were abrogated in brain-specific Nrf2-knockout mice subjected to TBI. These findings demonstrate that AS-Exos protects against TBI-induced oxidative stress and neuronal apoptosis by activating Nrf2 signaling in both rat and mouse models.

5.
Phys Rev Lett ; 127(11): 110506, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34558952

RESUMO

Active control of quantum systems enables diverse applications ranging from quantum computation to manipulation of molecular processes. Maximum speeds and related bounds have been identified from uncertainty principles and related inequalities, but such bounds utilize only coarse system information and loosen significantly in the presence of constraints and complex interaction dynamics. We show that an integral-equation-based formulation of conservation laws in quantum dynamics leads to a systematic framework for identifying fundamental limits to any quantum control scenario. We demonstrate the utility of our bounds in three scenarios-three-level driving, decoherence suppression, and maximum-fidelity gate implementations-and show that in each case our bounds are tight or nearly so. Global bounds complement local-optimization-based designs, illuminating performance levels that may be possible, as well as those that cannot be surpassed.

6.
J Nucl Med ; 2021 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34385337

RESUMO

Digital autoradiography (DAR) is a powerful tool to quantitatively determine the distribution of a radiopharmaceutical within a tissue section and is widely used in drug discovery and development. However, the low image resolution and significant background noise can result in poor correlation, even errors, between radiotracer distribution, anatomical structure, and molecular expression profiles. Differing from conventional optical systems, the point spread function (PSF) in DAR is determined by properties of radioisotope decay, phosphor and digitizer. Calibration of an experimental PSF a priori is difficult, prone to error, and impractical. We have developed a content-adaptive restoration algorithm to address these problems. Methods: We model the DAR imaging process using a mixed Poisson-Gaussian model, and blindly restore the image by a Penalized Maximum-Likelihood Expectation-Maximization algorithm (PG- PEM). PG-PEM implements a patch-based estimation algorithm with "Density-Based Spatial Clus- tering of Applications with Noise" to estimate noise parameters, and utilizes L2 and Hessian Frobenius (HF) norms as regularization functions to improve performance. Results: First, PG-PEM outperformed other restoration algorithms at the denoising task (p<0.01). Next, we implemented PG-PEM on pre-clinical DAR images (18F-FDG treated mice tumor and heart, 18F-NaF treated mice femur) and clinical DAR images (bone biopsy sections from 223RaCl2 treated castrate resistant prostate cancer patients). DAR images restored by PG-PEM of all samples achieved significantly higher effective resolution, contrast to noise ratio (CNR), and a lower standard deviation of background (STDB) (p<0.0001). Additionally, by comparing the registration results between the clinical DAR images and the segmented bone masks from the corresponding histological images, the radiopharmaceutical distribution was significantly improved (p<0.0001). Conclusion: PG-PEM is able to increase resolution and contrast while robustly accounting for DAR noise, and demonstrates the capacity to be widely implemented to improve pre- and clinical DAR imaging of radiopharmaceutical distribution.

7.
J Hazard Mater ; 421: 126639, 2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34396974

RESUMO

Developing catalysts with high activity, durability, and water resistance for ozone decomposition is crucial to regulate the pollution of ozone in the troposphere, especially in indoor air. To overcome the shortcomings of metal oxide catalysts with respect to their durability and water resistance, Fe-Co double-atom catalyst (DAC) is proposed as a novel catalyst for ozone decomposition. Here, through a systematic study using density functional theory (DFT) calculations and microkinetic modeling, the adsorption and catalytic decomposition of O3 on Fe-Co DAC have been examined based on adsorption configuration, orbital hybridization, and electron transfer. Based on Eley-Rideal (E-R) and Langmuir-Hinshelwood (L-H) reaction mechanisms, the mechanisms of ozone decomposition on Fe-Co DAC were explored by analyzing reaction paths and energy variations. To confirm the water-resistant of Fe-Co DAC, competitive adsorption behavior between O3 and dominant environmental gases was discussed through ab initio molecular dynamic (AIMD) simulation. The dominant reaction mechanism of ozone decomposition is L-H and the rate-determining step is the desorption of the first oxygen molecule from the surface of Fe-Co DAC which has an energy barrier of 0.78 eV. Due to this relatively low energy barrier and high turnover frequency (TOF), the optimal operation window of catalytic O3 decomposition on Fe-Co DAC is <500 K suggesting that catalytic decomposition of O3 on Fe-Co DAC can occur at room temperature. This theoretical study provides new insights for designing novel catalysts for ozone decomposition and fundamental guidance for subsequent experimental research.

8.
Mol Immunol ; 138: 172-180, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34438225

RESUMO

Interleukin-27 (IL-27), a member of the IL-6/IL-12 family, has diverse regulatory functions in various immune responses, and is recognised as a potent agonist and antagonist of CD4+T cells in different contexts. However, this dual role and underlying mechanisms have not been completely defined. In the present review, we summarise the dual role of IL-27 in CD4+T cells. In particular, we aimed to decipher its mechanism to better understand the context-dependent function of IL-27 in CD4+T cells. Furthermore, we propose a possible mechanism for the dual role of IL-27. This may be helpful for the development of appropriate IL-27 treatments in various clinical settings.

9.
Mol Ther ; 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34450253

RESUMO

The protein-coding ability of circular RNAs (circRNAs) has recently been a hot topic, but the expression and roles of protein-coding circRNAs in triple-negative breast cancer (TNBC) remain uncertain. By intersecting circRNA sequencing data from clinical samples and cell lines, we identified a circRNA, termed circ-EIF6, which predicted a poorer prognosis and correlated with clinicopathological characteristics in a cohort of TNBC patients. Functionally, we showed that circ-EIF6 promoted the proliferation and metastasis of TNBC cells in vitro and in vivo. Mechanistically, we found that circ-EIF6 contains a 675-nucleotide (nt) open reading frame (ORF) and that the -150-bp sequence from ATG functioned as an internal ribosome entry site (IRES), which is required for translation initiation in 5' cap-independent coding RNAs. circ-EIF6 encodes a novel peptide, termed EIF6-224 amino acid (aa), which is responsible for the oncogenic effects of circ-EIF6. The endogenous expression of EIF6-224aa was further examined in TNBC cells and tissues by specific antibody. Moreover, EIF6-224aa directly interacted with MYH9, an oncogene in breast cancer, and decreased MYH9 degradation by inhibiting the ubiquitin-proteasome pathway and subsequently activating the Wnt/beta-catenin pathway. Our study provided novel insights into the roles of protein-coding circRNAs and supported circ-EIF6/EIF6-224aa as a novel promising prognostic and therapeutic target for tailored therapy in TNBC patients.

10.
Bioengineered ; 12(1): 3201-3218, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34224308

RESUMO

Preterm birth (PTB) is an immune-inflammatory disease that needs to be resolved. This study aimed to identify the role of interleukin-27 (IL-27), an immunomodulatory factor, in PTB and its associated mechanisms. Here, we analyzed the high-throughput of samples data from the maternal-fetal interface to the peripheral circulation obtained from public databases and reported that the elevated IL-27 was involved with the onset of PTB. Further bioinformatics analyses (e.g. GeneMANIA and GSEA) revealed that IL-27 overexpression in the peripheral circulation as well as maternal-fetal interface is related to the activation of the immune-inflammatory process represented by IFN-γ signaling, etc. In addition, IL-27 and immune infiltration correlation analysis demonstrated that IL-27 mediates this immune-inflammatory imbalance, plausibly mainly through monocyte-macrophage and neutrophils. This finding was further validated by analyzing additional datasets. Overall, this is the first study to elaborate on the role of IL-27-mediated immuno-inflammation in PTB from the perspective of bioinformatics, which may provide a novel strategy for the prevention and treatment of PTB.

11.
Nat Ecol Evol ; 5(9): 1266-1272, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34211141

RESUMO

Proboscideans were keystone Cenozoic megaherbivores and present a highly relevant case study to frame the timing and magnitude of recent megafauna extinctions against long-term macroevolutionary patterns. By surveying the entire proboscidean fossil history using model-based approaches, we show that the dramatic Miocene explosion of proboscidean functional diversity was triggered by their biogeographical expansion beyond Africa. Ecomorphological innovations drove niche differentiation; communities that accommodated several disparate proboscidean species in sympatry became commonplace. The first burst of extinctions took place in the late Miocene, approximately 7 million years ago (Ma). Importantly, this and subsequent extinction trends showed high ecomorphological selectivity and went hand in hand with palaeoclimate dynamics. The global extirpation of proboscideans began escalating from 3 Ma with further extinctions in Eurasia and then a dramatic increase in African extinctions at 2.4 Ma. Overhunting by humans may have served as a final double jeopardy in the late Pleistocene after climate-triggered extinction trends that began long before hominins evolved suitable hunting capabilities.


Assuntos
Fósseis , Hominidae , África , Animais , Humanos
12.
Arch Gerontol Geriatr ; 96: 104477, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34280597

RESUMO

BACKGROUND: CircRNA circFADS2 has been reported to protect cells from LPS (Lipopolysaccharide)-induced cell injury, which contributes to osteoarthritis (OA). This study was carried out to study the role of circFADS2 in OA. METHODS: The expression of circFADS2 and miR-195-5p in synovial fluid samples from 63 OA patients and 63 healthy controls were detected by RT-qPCRs. The effects of overexpression of circFADS2 on the expression of miR-195-5p and the methylation of miR-195-5p gene in chondrocytes were evaluated by RT-qPCRs and MSPs, respectively. The roles of circFADS2 and miR-195-5p in regulating the LPS-induced apoptosis of chondrocytes were explored by cell apoptosis assay. RESULTS: CircFADS2 was downregulated in OA and inversely correlated with miR-195-5p. In chondrocytes, overexpression of circFADS2 and downregulation of miR-195-5p were observed after LPS treatment. In chondrocytes, overexpression of circFADS2 decreased the expression levels of miR-195-5p, and increased the methylation of miR-195-5p gene. Cell apoptosis assay showed that overexpression of circFADS2 reduced the apoptosis of chondrocytes under LPS treatment. Overexpression of miR-195-5p increased cell apoptosis and attenuated the effects of overexpression of circFADS2 on cell apoptosis. CONCLUSION: CircFADS2 may downregulate miR-19-5p through methylation to suppress LPS-induced apoptosis of chondrocytes, thereby suppressing OA.


Assuntos
MicroRNAs , Osteoartrite , Apoptose/genética , Condrócitos/metabolismo , Humanos , Lipopolissacarídeos , Metilação , MicroRNAs/genética , Osteoartrite/genética
13.
Huan Jing Ke Xue ; 42(7): 3304-3315, 2021 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-34212656

RESUMO

Urban surface runoff is an important non-point pollution source, and research on contamination characteristics of runoff is urgent for improving urban aquatic environment quality. Typical cities along the downstream Yangtze River, Wuxi and Nanjing, were selected in this study, and runoff samples from various underlying surfaces were collected to investigate contamination characteristics of heavy metals and dissolved organic matter (DOM). The results indicated that the concentration of heavy metals have exceed water quality standards in underlying surfaces partly, the decreasing order of metal contaminations was Zn > Cu > Pb > Sb > Cd. Contamination characteristics of surface runoff were found to exhibit significant differences, and arterial traffic exhibited the severe contamination of both heavy metals and DOM. Spectral characteristics indicated that the structure of DOM exhibited significant differences in various underlying surfaces, and DOM humification levels in Wuxi showed the following decreasing order:roof covering > sidewalk > parking area > grassy area > arterial traffic, aromaticity indexes exhibited order as:arterial traffic > grassy area > sidewalk > roof covering > parking area. Differential absorption spectroscopy (DAS) and FT-IR analyses indicated that DOM exhibited strong combination effects with cationic metal pollutants (Cu), which mainly occur with C=C structure and carboxylic and phenolic groups; DOM presented weak combination with anion metal pollutant (Sb), indicating that Sb likely exists as a free anion in runoff. The annual total loadings of metal contaminations in the study area were as follows:Zn > Cu > Pb > Sb > Cd. The loadings of Zn were 4.83 kg·a-1 and 3.21 kg·a-1 in Wuxi and Nanjing, respectively. Annual loadings of DOM reached 0.93 g·(m2·a)-1 and 8.72 g·(m2·a)-1. The results indicated that corresponding pollution reduction measures should be implemented for different underlying surfaces to reduce contaminant concentrations; our findings may provide essential information for improving the aquatic environment quality in downstream Yangtze River.


Assuntos
Metais Pesados , Poluentes Químicos da Água , China , Cidades , Monitoramento Ambiental , Metais Pesados/análise , Espectroscopia de Infravermelho com Transformada de Fourier , Poluentes Químicos da Água/análise
14.
Sci Total Environ ; 790: 148139, 2021 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-34098274

RESUMO

Water crisis across the globe has placed high pressure on social development due to the need to balance the water consumption between sustainable economy and functioning ecosystem. Integrated process-based modeling has been reported as an effective tool to better understand the complex mechanisms of water issues on a basin scale. Considering that it is still relatively difficult to simulate the water quantity-quality processes simultaneously, this study proposed an integrated modeling framework by coupling a hydrological model with a water quality model. Taking the Xiaoqing River Basin in the Shandong Province of northern China as an example, this study coupled a distributed hydrological model, SWAT, with a one-dimensional hydrodynamic-water quality model, HEC-RAS, to investigate its ability to simulate water quality and quality at the basin scale. The coupling of the two models adopted the "output-input" scheme, where the runoff modeling results from SWAT are input into HEC-RAS for hydrodynamic and water quality simulations of the river channel. The results show that the SWAT model can adequately reproduce runoff with accepted accuracy for the calibration and validation periods with acceptable R2 and Nash-Sutcliffe coefficients for the two hydrological stations. Further analysis also shows that the coupled model can simulate the concentration of ammonia nitrogen (NH4-N) and the chemical oxygen demand (COD) in the middle and upper stream of the river for both low and high flow periods. The coupling of the hydrological and hydraulic models in this study provides a good tool for identifying the spatial patterns of the water pollutants over the basin and, thus, helps simplify precision water management.


Assuntos
Ecossistema , Monitoramento Ambiental , China , Modelos Teóricos , Água , Qualidade da Água
16.
Curr Med Chem ; 2021 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-34126881

RESUMO

BACKGROUND: It is unclear whether angiotensin-converting enzyme inhibitors (ACEIs) in combination with angiotensin II receptor blockers (ARBs) are superior to ACEIs or ARBs alone in the treatment of nondiabetic chronic kidney disease (CKD). The present meta-analysis was designed to assess the efficacy and safety of ACEIs in combination with ARBs in nondiabetic CKD. METHODS: The PubMed, Embase, and Cochrane Library databases were searched to identify randomized controlled trials (RCTs) published prior to March 2020. A random-effects model was used to calculate the effect sizes of eligible studies. RESULTS: The present meta-analysis of 20 RCTs encompassing 1,398 patients with nondiabetic CKD demonstrated that ACEIs in combination with ARBs were superior to ACEIs or ARBs alone in reducing urine albumin excretion (SMD, -0.69; 95% CI, -1.13 to -0.25; P=0.002), urine protein excretion (SMD, -0.34; 95% CI, -0.46 to -0.23; P<0.001) and blood pressure (systolic blood pressure: WMD, -1.43; 95% CI, -2.42 to -0.44; P=0.005; diastolic blood pressure: WMD, -1.85; 95% CI, -2.67 to -1.04; P<0.001) without decreased glomerular filtration rate (SMD, -0.07; 95% CI, -0.20 to 0.06; P=0.30) or increased incidences of hyperkalaemia (RR, 1.25; 95% CI, 0.47 to RR=0.80; P=0.20 to 0.06; P=0.30). CONCLUSION: Compared with ACEIs or ARBs alone, ACEIs in combination with ARBs are effective and safe in the treatment of nondiabetic CKD. ACEIs combined with ARBs may be a better choice to reduce proteinuria as long as it can be tolerated. (PROSPERO number: CRD42020179398).

17.
Adv Sci (Weinh) ; 8(16): e2100965, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34174177

RESUMO

Rapid progress has been made to identify and study the causative agent leading to coronavirus disease 2019 (COVID-19) but many questions including who is most susceptible and what determines severity remain unanswered. Angiotensin-converting enzyme 2 (ACE2) is a key factor in the infection process of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). In this study, molecularly specific positron emission tomography imaging agents for targeting ACE2 are first developed, and these novel agents are evaluated in vitro, in preclinical model systems, and in a first-in-human translational ACE2 imaging of healthy volunteers and a SARS-CoV-2 recovered patient (NCT04422457). ACE2 expression levels in different organs in live subjects are quantitatively delineated and observable differences are measured in the patient recovered from COVID-19. Surprising sites of uptake in the breast, reproductive system and very low uptake in pulmonary tissues are reported. This novel method can add a unique tool to facilitate SARS-CoV-2 related research and improve understanding of this enigmatic disease. Molecular imaging provides quantitative annotation of ACE2, the SARS-CoV-2 entry receptor, to noninvasively monitor organs impacted by the COVID-19.


Assuntos
Enzima de Conversão de Angiotensina 2/metabolismo , COVID-19/metabolismo , COVID-19/virologia , Peptídeos/farmacocinética , SARS-CoV-2/metabolismo , Animais , COVID-19/patologia , Células Cultivadas , Feminino , Radioisótopos de Gálio/farmacocinética , Humanos , Masculino , Camundongos , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Ligação Proteica , SARS-CoV-2/isolamento & purificação , SARS-CoV-2/patogenicidade , Distribuição Tecidual , Ensaios Antitumorais Modelo de Xenoenxerto
18.
Hepatology ; 74(4): 2118-2132, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33999437

RESUMO

BACKGROUND AND AIMS: Liver ischemia reperfusion injury (IRI) remains an unresolved clinical problem. This study dissected roles of liver-resident macrophage Kupffer cells (KCs), with a functional focus on efferocytosis receptor T-cell immunoglobulin and mucin domain-containing protein-4 (TIM-4), in both the activation and resolution of IRI in a murine liver partial warm ischemia model. APPROACH AND RESULTS: Fluorescence-activated cell sorting results showed that TIM-4 was expressed exclusively by KCs, but not infiltrating macrophages (iMФs), in IR livers. Anti-TIM-4 antibody depleted TIM-4+ macrophages in vivo, resulting in either alleviation or deterioration of liver IRI, which was determined by the repopulation kinetics of the KC niche with CD11b+ macrophages. To determine the KC-specific function of TIM-4, we reconstituted clodronate-liposome-treated mice with exogenous wild-type or TIM-4-deficient KCs at either 0 hour or 24 hours postreperfusion. TIM-4 deficiency in KCs resulted in not only increases in the severity of liver IRI (at 6 hours postreperfusion), but also impairment of the inflammation resolution (at 7 days postreperfusion). In vitro analysis revealed that TIM-4 promoted KC efferocytosis to regulate their Toll-like receptor response by up-regulating IL-10 and down-regulating TNF-α productions. CONCLUSIONS: TIM-4 is critical for KC homeostatic function in both the activation and resolution of liver IRI by efferocytosis.

19.
World J Surg Oncol ; 19(1): 138, 2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-33941214

RESUMO

BACKGROUND: To investigate the influence of different risk factors on central lymph node metastasis (CLNM) in the cervical region in patients with papillary thyroid carcinoma (PTC). METHODS: This retrospective study included 2586 PTC patients. Potential risk factors were identified by univariate analysis, and the relationships between these factors and CLNM were ascertained by multivariable analysis. A scoring system was constructed, and the optimal cut-off value was determined. RESULTS: On univariate analysis, sex, age, tumor diameter, multifocality, capsule invasion, vascular invasion, total number of lymph nodes in the central region, and serum thyroid peroxidase antibody (TPOAb) concentration were identified as potential risk factors for CLNM in the cervical region, whereas nerve invasion, thyroid-stimulating hormone concentration, and thyroglobulin antibody (TgAb) concentration were not. Multivariable analysis indicated that male sex, young age, large tumor diameter, multifocality, vascular invasion, a large number of central lymph nodes, and a low TPOAb concentration were significant risk factors. From these factors, a preoperative CLNM risk assessment scale was constructed for predicting CLNM in the cervical region for PTC patients. CONCLUSION: Male sex, young age, large tumor diameter, multifocality, vascular invasion, a large number of central lymph nodes, and a low TPOAb concentration were positively correlated with CLNM in the cervical region in PTC patients. The preoperative CLNM risk assessment scale based on these risk factors is expected to offer accurate preoperative assessment of central lymph node status in PTC patients.


Assuntos
Carcinoma Papilar , Neoplasias da Glândula Tireoide , Carcinoma Papilar/cirurgia , Humanos , Linfonodos/cirurgia , Metástase Linfática , Masculino , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Câncer Papilífero da Tireoide/cirurgia , Neoplasias da Glândula Tireoide/cirurgia
20.
J Crohns Colitis ; 2021 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-33987665

RESUMO

Macrophages in the intestinal mucosa can rapidly engage Toll-like receptor (TLR)-mediated inflammatory responses to defect against pathogen invasion, but these same innate immune responses can also drive the induction of colitis. Our previous research revealed that metadherin (MTDH), is overexpressed in multiple cancers and plays vital roles in tumor progression. However, the role of MTDH in intestinal inflammation is largely unknown. In this study, we found the MTDH expression in colonic lamina propria (CLP) macrophages was positively correlated with inflammatory colitis severity. MTDH -/- mice were protected against the symptoms of DSS-induced colitis, however, adoptive transfer of MTDH wild-type (WT) monocytes partially restored the susceptibility of MTDH -/- mice to DSS-induced colitis. TLR stimulation was sufficient to induce the expression of MTDH, whereas the absence of MTDH was sufficient to suppress TLR-induced production of inflammatory cytokines by macrophages. From a mechanistic perspective, MTDH recruited TRAF6 to TAK1, leading to TRAF6-mediated TAK1 K63 ubiquitination and phosphorylation, ultimately facilitating TLR-induced NF-κB and MAPK signaling. Taken together, our results indicate that MTDH contributes to colitis development by promoting TLR induced proinflammatory cytokines production in CLP macrophages and might represent a potential therapeutic approach for intestine inflammation intervention.

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