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1.
J Environ Manage ; 325(Pt A): 116454, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36252328

RESUMO

Optimized fertilization is an effective strategy for improving nitrogen (N) use efficiency and maintaining high crop yield, but its long-term impacts on soil organic carbon (C) and inorganic N dynamics remain unclear. The objectives of this study were to 1) explore the economic optimum N rate and evaluate the DSSAT CERES-Maize model using the measurements from three 3-year maize (Zea mays L.) field experiments, in Gongzhuling and Yushu County, Northeast China, and 2) assess the long-term impacts of farmers' N rate (N250), optimum N rate (N180) and organic-inorganic combined N rate (MN180) on maize yields, soil N and C changes from 1985 to 2020. Results showed that similar maize yields of 8000-11,000 kg ha-1 were achieved under the average economic optimum N rate of 170 kg N ha-1 relative to N250 in both counties. Good agreements were observed between the simulated and measured maize yield, above-ground biomass, N uptake and soil nitrate (NO3--N). Long-term simulation confirmed that N180 and MN180 can achieve the same yield as N250 in both counties. The lowest annual soil inorganic N balance, NO3--N leaching, and nitrous oxide (N2O) and ammonia (NH3) emissions were achieved under MN180, followed by N180 in both sites. Higher NO3--N leaching was found in sandy clay loam soil than silt clay loam and clay loam soils. Average soil organic C (SOC, 0-0.2 m) increased from 1.3 to 2.4% in Gongzhuling and from 2.2 to 2.4% in Yushu under MN180 during the 35-year period, but it showed declining trends under N180 and N250. We concluded that the economic optimum N rate could be an option to replace current farmers' N rate for the continuous maize. Substitution of inorganic fertilizer by 20-30% manure under the optimum N rate showed advantage on maintaining high yield, reducing soil inorganic N losses as well as increasing SOC stock for sustainable agriculture.


Assuntos
Solo , Zea mays , Carbono/análise , Argila , Fertilizantes/análise , Agricultura/métodos , Nitrogênio/análise , Fertilização , China
2.
Food Chem ; 399: 133991, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36037681

RESUMO

Fish oil develops particular off-odors, mainly fishy odor, from the oxidation of its characteristic fatty acids, docosahexaenoic (DHA) and eicosapentaenoic (EPA). Anchovy oil (AO) was taken as representative of fish oils. This was compared to three vegetable oils with different fatty acid compositions, i.e. camellia, sunflower and linseed oil, and differential volatile compounds were identified by static-headspace gas-chromatography ion-mobility-spectrometry (SHS-GC-IMS) and orthogonal partial-least-squares discriminant analysis (OPLS-DA) during oxidation at 60 °C. Three groups of differential volatile compounds detected at higher concentrations in the AO were screened out and two compounds, identified as 5-methylfurfural and 2-acetylfuran, were characteristic to the AO and not found in the vegetable oils. They were formed from both EPA and DHA, only present in the AO, and their formation mechanisms were proposed. The contents of 5-methylfurfural and 2-acetylfuran increased linearly with the oxidation time and consequently they could be used as oxidative markers of fish oils.


Assuntos
Quimiometria , Óleos de Peixe , Ácidos Graxos/análise , Óleos de Peixe/química , Furaldeído/análogos & derivados , Furanos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Óleos Vegetais
3.
Food Chem ; 404(Pt B): 134670, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36323023

RESUMO

Major yolk proteins (MYPs), one class of the main abundant proteins in sea cucumber body wall, seem to garner more attention in recent years. Herein, a method using multiple reactions monitoring mass spectrometry (MRM-MS) was deliberatively developed to perform quantification analysis of three MYPs, i.e. BAH79576.1, BAH79577.1 and PIK45784.1. Contents of MYPs in body wall of fresh and dried sea cucumbers as well as in waste liquid of boiling and steaming were determined using their corresponding signature peptides of VDEFTGIVGSLR, KLDMYPPPLAR, LDMYPPPLAR, and SGHGEVMFVDSK. The loss of MYPs in the processing of sea cucumbers was directly verified by quantitation data of MYPs in sea cucumber body wall and the waste liquids. This study not only evidenced the loss of MYPs during the processing of sea cucumbers, but also implicated the potential of recycling MYPs from the processing waste water, providing helpful suggestions in maximizing the value of sea cucumbers.

4.
Food Chem ; 404(Pt B): 134724, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36332587

RESUMO

The aim of the present research was to explore the development of off-odors in fish oil from the perspective of fatty acid oxidation. It was found that the off-odors elicited by the two major ω-3 PUFAs in fish oil, i.e. DHA and EPA, were different from those by fish oil. Results showed that simultaneous oxidation of fatty acids other than DHA and EPA can be involved. The off-odors of fish oil was successfully simulated by combining oxidized samples of DHA, EPA and sunflower oil. Therefore, oxidation of oleic and linoleic acids also contributed to the off-odors in fish oil. A novel analytical approach that consisted in the combination of gas chromatography-ion mobility spectrometry (GC-IMS) and orthogonal partial least squares discriminant analysis (OPLS-DA) was applied to identify differences in the volatile components between the recombinant oil and the fish oil.

5.
J Oncol ; 2022: 5873002, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36339647

RESUMO

Background: Activation of chronic hepatitis B virus (HBV) infection is an important cause of acute-on-chronic liver failure (ACLF). However, the effect of HBV-ACLF episode on hepatocellular carcinoma (HCC) occurrence remains largely unknown. Methods: A total of 769 HBV-ACLF patients and 2114 HBV-related chronic liver disease (HBV-CLD) patients diagnosed between August 1998 and December 2011 were enrolled in this prospective cohort study. Of the HBV-CLD patients, 380 received lifetime antiviral treatment with nucleos(t)ide analogues. Propensity score matching was applied to reduce baseline differences between HBV-ACLF and HBV-CLD cohorts. Results: The survival rate of HBV-ACLF patients was 53.6%, 50.3%, 47.8%, and 46.2% at 90-day, 1-year, 5-year, and 10-year, respectively. The cumulative incidence of HCC was lower in HBV-ACLF cohort with 369 eligible patients survived for >90 days than in HBV-CLD cohort with the 380 patients (5.77/1,000 vs. 9.78/1,000 person-years, p = 0.0497). HBV-ACLF episode decreased HCC risk regardless of liver cirrhosis, and in patients without family history of HCC. Multivariate Cox analyses indicated that male, increasing age, liver cirrhosis, and platelet count (≤100 × 109/L) increased, whereas HBV-ACLF episode decreased, HCC risk independently. In the propensity score-matched cohorts, HBV-ACLF episode reduced HCC incidence (10.20/1,000 vs. 4.66/1,000 person-years, p = 0.0326). The area under curve of nomogram was 0.812 for 3-year HCC probability. Conclusions: HBV-ACLF episode decreases HCC occurrence in chronic HBV patients. Older age and liver cirrhosis independently increased HCC occurrence. A nomogram-enrolled episode of ACLF reliably predicts the occurrence of HCC.

6.
Cell Death Dis ; 13(11): 926, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36335087

RESUMO

As the most important RNA epigenetic regulation in eukaryotic cells, N6-metheyladenosine (m6A) modification has been demonstrated to play significant roles in cancer progression. However, this modification in long intergenic non-coding RNAs (lincRNAs) and the corresponding functions remain elusive. Here, we showed a lincRNA LINC02551 was downregulated by AlkB Homolog 5 (ALKBH5) overexpression in a m6A-dependent manner in hepatocellular carcinoma (HCC). Functionally, LINC02551 was required for the growth and metastasis of HCC. Mechanistically, LINC02551, a bona fide m6A target of ALKBH5, acted as a molecular adaptor that blocked the combination between DDX24 and a E3 ligase TRIM27 to decrease the ubiquitination and subsequent degradation of DDX24, ultimately facilitating HCC growth and metastasis. Thus, ALKBH5-mediated LINC02551 m6A methylation was required for HCC growth and metastasis.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , RNA Longo não Codificante , Humanos , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/patologia , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Homólogo AlkB 5 da RNA Desmetilase/genética , Homólogo AlkB 5 da RNA Desmetilase/metabolismo , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/patologia , Epigênese Genética , RNA Helicases DEAD-box/metabolismo
7.
Cell Death Dis ; 13(11): 917, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36319631

RESUMO

Thioredoxin reductase 1 (TXNRD1) is one of the major redox regulators in mammalian cells, which has been reported to be involved in tumorigenesis. However, its roles and regulatory mechanism underlying the progression of HCC remains poorly understood. In this study, we demonstrated that TXNRD1 was significantly upregulated in HCC tumor tissues and correlated with poor survival in HCC patients. Functional studies indicated TXNRD1 knockdown substantially suppressed HCC cell proliferation and metastasis both in vitro and in vivo, and its overexpression showed opposite effects. Mechanistically, TXNRD1 attenuated the interaction between Trx1 and PTEN which resulting in acceleration of PTEN degradation, thereby activated Akt/mTOR signaling and its target genes which conferred to elevated HCC cell mobility and metastasis. Moreover, USF2 was identified as a transcriptional suppressor of TXNRD1, which directly interacted with two E-box sites in TXNRD1 promoter. USF2 functioned as tumor suppressor through the downstream repression of TXNRD1. Further clinical data revealed negative co-expression correlations between USF2 and TXNRD1. In conclusion, our findings reveal that USF2-mediated upregulation of TXNRD1 contributes to hepatocellular carcinoma progression by activating Akt/mTOR signaling.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Animais , Humanos , Carcinoma Hepatocelular/patologia , Tiorredoxina Redutase 1/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Hepáticas/patologia , Regulação para Cima , Proliferação de Células , Serina-Treonina Quinases TOR/metabolismo , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica , Mamíferos , Fatores Estimuladores Upstream/genética
8.
BMC Health Serv Res ; 22(1): 1341, 2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36371216

RESUMO

BACKGROUND: Segmentation models such as latent class analysis are an increasingly popular approach to inform group-tailored interventions for high-risk complex patients. Multiple studies have identified clinically meaningful high-risk segments, but few have evaluated change in groupings over time. OBJECTIVES: To describe population-level and individual change over time in latent comorbidity groups among Veterans at high-risk of hospitalization in the Veterans Health Administration (VA). RESEARCH DESIGN: Using a repeated cross-sectional design, we conducted a latent class analysis of chronic condition diagnoses. We compared latent class composition, patient high-risk status, and patient class assignment in 2018 to 2020. SUBJECTS: Two cohorts of eligible patients were selected: those active in VA primary care and in the top decile of predicted one-year hospitalization risk in 2018 (n = 951,771) or 2020 (n = 978,771). MEASURES: Medical record data were observed from January 2016-December 2020. Latent classes were modeled using indicators for 26 chronic health conditions measured with a 2-year lookback period from study entry. RESULTS: Five groups were identified in both years, labeled based on high prevalence conditions: Cardiometabolic (23% in 2018), Mental Health (18%), Substance Use Disorders (16%), Low Diagnosis (25%), and High Complexity (10%). The remaining 8% of 2018 patients were not assigned to a group due to low predicted probability. Condition prevalence overall and within groups was stable between years. However, among the 563,725 patients identified as high risk in both years, 40.8% (n = 230,185) had a different group assignment in 2018 versus 2020. CONCLUSIONS: In a repeated latent class analysis of nearly 1 million Veterans at high-risk for hospitalization, population-level groups were stable over two years, but individuals often moved between groups. Interventions tailored to latent groups need to account for change in patient status and group assignment over time.


Assuntos
Veteranos , Humanos , Estudos Transversais , Comorbidade , Doença Crônica , Atenção Primária à Saúde
9.
Front Public Health ; 10: 1019198, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36408017

RESUMO

Background: Understanding multidrug-resistant tuberculosis (MDR-TB) transmission patterns is crucial for controlling the disease. We aimed to identify high-risk populations and geographic settings of MDR-TB transmission. Methods: We conducted a population-based retrospective study of MDR-TB patients in Beijing from 2018 to 2020, and assessed MDR-TB recent transmission using whole-genome sequencing of isolates. Geospatial analysis was conducted with kernel density estimation. We combined TransPhylo software with epidemiological investigation data to construct transmission networks. Logistic regression analysis was utilized to identify risk factors for recent transmission. Results: We included 241 MDR-TB patients, of which 146 (60.58%) were available for genomic analysis. Drug resistance prediction showed that resistance to fluoroquinolones (FQs) was as high as 39.74% among new cases. 36 (24.66%) of the 146 MDR strains were grouped into 12 genome clusters, suggesting recent transmission of MDR strains. 44.82% (13/29) of the clustered patients lived in the same residential community, adjacent residential community or the same street as other cases. The inferred transmission chain found a total of 6 transmission events in 3 clusters; of these, 4 transmission events occurred in residential areas and nearby public places. Logistic regression analysis revealed that being aged 25-34 years-old was a risk factor for recent transmission. Conclusions: The recent transmission of MDR-TB in Beijing is severe, and residential areas are common sites of transmission; high levels of FQs drug resistance suggest that FQs should be used with caution unless resistance can be ruled out by laboratory testing.


Assuntos
Mycobacterium tuberculosis , Tuberculose Resistente a Múltiplos Medicamentos , Humanos , Adulto , Pequim/epidemiologia , Estudos Retrospectivos , Mycobacterium tuberculosis/genética , Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia , Tuberculose Resistente a Múltiplos Medicamentos/genética , Genômica
10.
Curr Res Food Sci ; 5: 2171-2177, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36387594

RESUMO

The liver is easily injured by exogenous chemicals through reactive oxygen species (ROS), which lead to ferroptosis, a ROS-dependent programmed cell death characterized by iron accumulation and lipid peroxidation. However, whether iron restriction has a positive role in chemicals-induced liver injuries is unknown. The present study investigated the effects of an iron-deficient diet on liver injuries induced by alcohol or diethylnitrosamine (DEN). Mice were fed an iron-deficient diet for four weeks, then treated with three doses of alcohol (5 g/kg, 24 h interval, gavage) to mimic mild liver injury or five doses of DEN (50 mg/kg, 24 h interval, i. p.) to mimic severe liver failure. The results showed that mice were iron-deficient after four weeks of feeding. Interestingly, as evaluated by H&E staining of liver slices, liver/body weight ratio, serum ALT and AST, iron deficiency significantly alleviated liver injuries triggered by alcohol or DEN. The activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), and the expression of CYP2E1 were increased by iron deficiency. Mechanistically, iron deficiency prevented the decrease of glutathione peroxidase 4 (GPX4), which eliminated malondialdehyde (MDA) by utilizing glutathione (GSH). In summary, alcohol- or DEN-induced liver injuries were mitigated by the iron-deficient diet by inhibiting ferroptosis, which might be a promising measure for preventing liver injuries induced by alcohol, DEN, or other exogenous chemicals.

11.
Sci Total Environ ; 858(Pt 2): 159877, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36343802

RESUMO

Condensable particulate matter (CPM), as an air pollutant that has received wide attention in recent years, has a high emission concentration compared to filterable particulate matter (FPM), yet there is not a well-developed removal method. Air pollution control devices (APCDs) with a condensation process have a certain effect on CPM removal, which inspired us to study the condensation behavior of CPM. During the condensation process, the condensed CPM may exist in two final forms: one was collected by the cold surface that caused the condensation; the other was converted to fine particles and suspended in the space of the flue. In a sense, the surface collection form can reflect the removal of CPM, while the CPM in the space suspension form should be further separated with the aim of removal. In this work, we adopted a CPM sampling system based on EPA Method 202 to reveal the distribution of the condensation behavior of CPM. In this sampling system, the CPM collected by all the cooling surfaces, including the cooling coil and impingers, can be counted as the surface collection form, while those collected by the terminal CPM filter can be regarded as the space suspension form. It was found that about 75 % of CPM was collected by the cooling surfaces, which suggested that CPM preferred to be in the surface collection form than the space suspension form. This preference characteristic also could be observed in the inorganic (CPMi) and organic components of the CPM (CPMo). Among the CPMi, almost all NH4+ and SO42- condensed in the form of surface collection. The preference characteristics in CPM's (and its components') condensation behavior are similar under every temperature reduction condition. In this work, the interference of CPM measurement error was resolved by the statistical method of ANOVA.

12.
JAMA Cardiol ; 7(11): e223042, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36350316

RESUMO

This case report describes a diagnosis of cardiac tamponade secondary to ruptured coronary artery aneurysm in a man who presented with acute chest pain and dyspnea.


Assuntos
Aneurisma Roto , Tamponamento Cardíaco , Aneurisma Coronário , Masculino , Humanos , Tamponamento Cardíaco/etiologia , Vasos Coronários/diagnóstico por imagem , Aneurisma Coronário/diagnóstico , Aneurisma Coronário/diagnóstico por imagem , Aneurisma Roto/complicações , Aneurisma Roto/diagnóstico por imagem , Aneurisma Roto/cirurgia
13.
IEEE Trans Med Imaging ; PP2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36327189

RESUMO

Main coronary segmentation from the X-ray angiography images is important for the computer-aided diagnosis and treatment of coronary disease. However, it confronts the challenge at three different image granularities (the semantic, surrounding, and local levels). The challenge includes the semantic confusion between the main and collateral vessels, low contrast between the foreground vessel and background surroundings, and local ambiguity near the vessel boundaries. The traditional hand-crafted feature-based methods may be insufficient because they may lack the semantic relationship information and may not distinguish the main and collateral vessels. The existing deep learning-based methods seem to have issues due to the deficiency in the long-distance semantic relationship capture, the foreground and background interference adaptability, and the boundary detail information preservation. To solve the main coronary segmentation challenge, we propose the progressive perception learning (PPL) framework to inspect these three different image granularities. Specifically, the PPL contains the context, interference, and boundary perception modules. The context perception is designed to focus on the main coronary vessel based on the semantic dependence capture among different coronary segments. The interference perception is designed to purify the feature maps based on the foreground vessel enhancement and background artifact suppression. The boundary perception is designed to highlight the boundary details based on boundary feature extraction through the intersection between the foreground and background predictions. Extensive experiments on 1085 subjects show that the PPL is effective (e.g., the overall Dice is greater than 95%), and superior to thirteen state-of-the-art coronary segmentation methods.

14.
Front Med (Lausanne) ; 9: 957478, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388878

RESUMO

Background: Sleeve gastrectomy is an effective bariatric procedure; however, sleeve gastrectomy-related adverse skeletal outcomes have been increasingly reported. High levels of sex hormone-binding globulin (SHBG) have been documented to be a risk factor of bone mineral density (BMD) loss with different effects observed between sexes. The aim of this study was to identify sex-specific changes in BMD following sleeve gastrectomy and to evaluate the role of SHBG in this process. Methods: This retrospective study included 19 middle-aged men and 30 non-menopausal women with obesity who underwent sleeve gastrectomy in China. Anthropometrics, bone turnover markers, calciotropic hormones, BMD, SHBG, and gonadal steroids were measured preoperatively and at 6 and 12 months postoperatively. Longitudinal changes in BMD, bone turnover markers and SHBG were compared between sexes by linear mixed models. Multiple stepwise regression analysis was used to identify the predictors of BMD loss at the investigated bone sites. Results: Over the 12-month study period, total hip and femoral neck BMD decreased, while lumbar spine BMD remained largely unchanged in both sexes. Linear mixed models revealed significant sex × time interaction effects in total hip BMD and SHBG, showing that men had a significantly greater reduction in total hip BMD and less increase in SHBG after sleeve gastrectomy than women. In the multivariate model, SHBG was significantly associated with total hip BMD loss in men (adjusted ß = -0.533, P = 0.019) but not women while total estrogen was significantly associated with total hip BMD loss in women (adjusted ß = 0.508, P = 0.01) but not men. Conclusion: Significant sex-specific BMD changes were observed after sleeve gastrectomy in the current study. Sleeve gastrectomy-related increase in SHBG may be a specific risk factor for total hip BMD loss in men. Our results indicate that sex-specific screening may be warranted to facilitate personalized postoperative bone care in this population.

15.
Liver Int ; 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36385489

RESUMO

BACKGROUND: Long non-coding RNAs (LncRNAs) have been demonstrated to associate with a variety of cancers. However, the mechanisms of LncRNAs in hepatocellular carcinoma (HCC) progression are still not fully clarified. METHODS: LINC01608 expression level in HCC and adjacent normal tissues was detected by real-time-quantitively PCR (RT-qPCR) in clinical samples and in situ hybridization (ISH) in tissue microarray. Several functional assays were performed to determine the biological effects of LINC01608 in HCC cells in vitro, while subcutaneous xenograft models and lung metastasis models in nude mice and immunohistochemistry (IHC) results showed the role of LINC01608 in HCC progression in vivo. The combination of LINC01608 with miR-875-5p and target genes was elucidated by dual-luciferase report assays, RNA immunoprecipitation (RIP) assays and fluorescence in situ hybridization (FISH) assays. Finally, bioinformatics analysis and chromatin immunoprecipitation (CHIP) were performed to investigate the mechanism of Yin Yang-1 (YY1) regulating LINC01608 transcription. RESULTS: LINC01608 was overexpressed in HCC tissues, and high LINC01608 expression predicted poor overall survival (OS) and disease-free survival (DFS) in HCC patients. LINC01608 could promote HCC cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) in vitro and in vivo. Furthermore, we demonstrated that LINC01608 could sponge to miR-875-5p and activate the EGFR/ERK pathway. Moreover, we identified transcriptional factor YY1 could bind to the promoter of LINC01608 and induce its transcription. CONCLUSION: LINC01608 could serve as a promising prognostic biomarker of HCC. YY1-activated LINC01608 could promote HCC progression by associating with miR-875-5p to induce the EGFR/ERK signalling pathway. This discovery might provide therapeutic strategies for HCC.

16.
Resour Policy ; : 103098, 2022 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-36340700

RESUMO

The COVID-19 pandemic has led to extensive news coverage, causing investor sentiment to swing, which has further increased financial market price volatility. There is an increasing need to find a hedge against sentiment risk. This paper examines the hedge capabilities of gold and Bitcoin against COVID-19-related news sentiment (CNS) risk under a nonlinear autoregressive distributed lag (NARDL) model. Our empirical results reveal that there is an obvious asymmetric effect from the CNS on gold prices in the short run and that the decrease in the COVID-19-related news index would have a greater impact on gold prices than when it increases. The impact of CNS on Bitcoin prices is asymmetric in the long and short term, especially in the long term. In addition, we conclude that gold is a hedge against CNS risk in the long term, and the hedging effect of Bitcoin is mainly reflected in the short-term.

17.
Oncogene ; 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36380240

RESUMO

Transforming growth factor beta (TGF-ß) signaling pathway plays important roles in hepatocellular carcinoma (HCC) progression. Long intergenic non-protein coding RNAs (lincRNAs) are important components of TGF-ß signaling pathway and perform their functions through different mechanisms. Here, we found that LINC02551 was activated by TGF-ß transcriptionally and identified a 174-amino-acid peptide, Jun binding micropeptide (JunBP), encoded by LINC02551 in HCC tissues and HCC cell lines. Functional study showed that JunBP promotes HCC metastasis through binding to c-Jun and subsequent promotion of its phosphorylated activation. Activated c-Jun has higher binding affinity to SMAD3, which in turn leads to more SMAD3 recruited to the promoter region of LINC02551. We find a positive feedback among them, and this mechanism provides a novel potential prognostic biomarker and therapeutic target in HCC.

18.
ACS Nano ; 16(11): 17572-17592, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36331385

RESUMO

Single atom catalysts (SACs) have been attracting extensive attention in electrocatalysis because of their unusual structure and extreme atom utilization, but the low metal loading and unified single site induced scaling relations may limit their activity and practical application. Tailoring of active sites at the atomic level is a sensible approach to break the existing limits in SACs. In this review, SACs were first discussed regarding carbon or non-carbon supports. Then, five tailoring strategies were elaborated toward improving the electrocatalytic activity of SACs, namely strain engineering, spin-state tuning engineering, axial functionalization engineering, ligand engineering, and porosity engineering, so as to optimize the electronic state of active sites, tune d orbitals of transition metals, adjust adsorption strength of intermediates, enhance electron transfer, and elevate mass transport efficiency. Afterward, from the angle of inducing electron redistribution and optimizing the adsorption nature of active centers, the synergistic effect from adjacent atoms and recent advances in tailoring strategies on active sites with binuclear configuration which include simple, homonuclear, and heteronuclear dual atom catalysts (DACs) were summarized. Finally, a summary and some perspectives for achieving efficient and sustainable electrocatalysis were presented based on tailoring strategies, design of active sites, and in situ characterization.

19.
Meat Sci ; 196: 109044, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36410055

RESUMO

This study was designed to investigate the utilization of seaweed dietary fiber (SDF) as a potential alternative to phosphates on the quality profiles of phosphate-free frankfurters. The results showed that the incorporation of SDF obviously promote the textural parameters of phosphate-free frankfurters, which was effectively verified by scanning electron microscopy. Meanwhile, SDF effectively retarded lipid oxidation of phosphate-free frankfurters during storage (P < 0.05). Moreover, 1.00% SDF was proven to show the best optimal phosphates replacing effect, as well as overcome the quality defects of phosphate-free frankfurters to the maximum extent. In addition, hydrogen bonding and hydrophobic interactions were the primary molecular force in SDF-added phosphate-free frankfurters. Therefore, our results implied that SDF can be considered as a feasible alternative for the processing of phosphate-free frankfurters with improved quality profile and superior health benefits which consistent with the demand of healthier meat products.

20.
Sci Rep ; 12(1): 20299, 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36434095

RESUMO

Rockbursts are some of the most severe dynamic disasters in coal mines. In this paper, the discrimination method of the tectonic stress field is proposed by analyzing the modern stress field in China. The tectonic stress field formed by modern tectonic movement guides in situ stress measurements. According to the stress state classification, most rockbursts in coal mines in China are closely associated with tectonic stress. For tectonic stress-driven rockbursts, modern tectonic movement and modern tectonic stress fields must be considered. The stress change and energy transfer caused by tectonic movement affect the geological structure where coal mines are located. Energy accumulation under rockburst conditions is mainly formed by natural geo-dynamic movement and the mining configuration, and energy accumulation is the basis for rockbursts. The application of the geo-dynamic environmental evaluation method to determine the coalfield geo-dynamic process and the influence of modern tectonic movement is proposed. Accordingly, the classification method of rockbursts in coal mines is established. Based on the distribution characteristics of modern tectonic conditions in China, it is revealed that these dynamic disasters follow a "110" distribution. Finally, a "three condition" criteria of rockbursts is proposed: the geo-dynamic environment is a necessary condition for rockbursts, mining disturbance is a sufficient condition for rockbursts, and risk-releasing measures are a condition controlling rockburst risk mitigation.

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