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Microorganisms ; 8(5)2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32443502


Growing concern for public health and food safety has prompted a special interest in developing nutritional strategies for removing waterborne and foodborne pathogens, including Salmonella. Strong links between manganese (Mn) and intestinal barrier or immune function hint that dietary Mn supplementation is likely to be a promising approach to limit the loads of pathogens in broilers. Here, we provide evidence that Salmonella Typhimurium (S. Typhimurium, 4 × 108 CFUs) challenge-induced intestinal injury along with systemic Mn redistribution in broilers. Further examining of the effect of dietary Mn treatments (a basal diet plus additional 0, 40, or 100 mg Mn/kg for corresponding to Mn-deficient, control, or Mn-surfeit diet, respectively) on intestinal barrier and inflammation status of broilers infected with S. Typhimurium revealed that birds fed the control and Mn-surfeit diets exhibited improved intestinal tight junctions and microbiota composition. Even without Salmonella infection, dietary Mn deficiency alone increased intestinal permeability by impairing intestinal tight junctions. In addition, when fed the control and Mn-surfeit diets, birds showed decreased Salmonella burdens in cecal content and spleen, with a concomitant increase in inflammatory cytokine levels in spleen. Furthermore, the dietary Mn-supplementation-mediated induction of cytokine production was probably associated with the nuclear factor kappa-B (NF-κB)/hydrogen peroxide (H2O2) pathway, as judged by the enhanced manganese superoxide dismutase activity and the increased H2O2 level in mitochondria, together with the increased mRNA level of NF-κB in spleen. Ingenuity-pathway analysis indicated that acute-phase response pathways, T helper type 1 pathway, and dendritic cell maturation were significantly activated by the dietary Mn supplementation. Our data suggest that dietary Mn supplementation could enhance intestinal barrier and splenic inflammatory response to fight against Salmonella infection in broilers.

J Anim Physiol Anim Nutr (Berl) ; 103(1): 53-63, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30328153


The present study was conducted to investigate the effects of dietary vitamin level on sternum growth, calcification and carcass traits in meat duck. A total of 432 1-d-old mixed-sex Cherry Valley ducks (216 males and 216 females) were randomly allocated and fed low-vitamin level diet (70% NRC vitamin regimen), high-vitamin level diet (DSM vitamin regimen) or medium-vitamin level diet (50% low-vitamin level diet and 50% high-vitamin level diet). Sternum and serum were harvested after 49 d of feeding. Compared with the low-vitamin level group, dietary high-vitamin level increased body weight (BW) at d 49 (p = 0.029) but did not alter all parameters of carcass trait (p > 0.05). Medium- and high-vitamin level increased sternum defatted weight, density, ash and calcium (Ca) concentration (p < 0.05). Meanwhile, the medium and high-vitamin level group significantly decreased the relative proportions of the keel cartilage at 49 d (p < 0.05) and decreased the sternum length and height (p < 0.05) in meat ducks at 49 d. Likewise, high-vitamin level improved serum Ca and phosphate (P) content (p < 0.05) and declined serum Alkaline phosphatase (ALP) activity (p = 0.003) compared with the low-vitamin level group. Our study indicates that high-vitamin level did not affect the examined carcass traits; however, high-vitamin level improved growth performance and sternum calcification.

Densidade Óssea/efeitos dos fármacos , Desenvolvimento Ósseo/efeitos dos fármacos , Patos/crescimento & desenvolvimento , Vitaminas/farmacologia , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Composição Corporal , Dieta/veterinária , Feminino , Masculino , Distribuição Aleatória , Vitaminas/administração & dosagem
Br J Nutr ; 120(11): 1217-1229, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30309398


Both genetic selection and increasing nutrient density for improving growth performance had inadvertently increased leg problems of meat ducks, which adversely affects animal welfare. We hypothesised that slowing weight gain with improving tibia quality probably enhanced tibial mechanical properties and alleviated leg deformities. Therefore, the present study aimed to evaluate the effect of graded Ca supplementation in a low-nutrient density (LND) diet on tibia composition and bone turnover in meat ducks. A total of 720 15-d-old male meat ducks were randomly assigned and fed a standard nutrient density positive control (PC) diet containing 0·9 % Ca, and four LND diets with 0·5, 0·7, 0·9 and 1·1 % Ca, respectively. Ducks fed the 0·5 % Ca LND diet and the PC diet had higher incidence of tibial dyschondroplasia (TD). When compared with the 0·5 % Ca LND diet, LND diets with ≥0·7 % Ca significantly improved tibia composition, microarchitecture and mechanical properties, and consequently decreased the incidence of TD. Furthermore, LND diets with ≥0·7 % Ca increased osteocyte-specific gene mRNA expression, blocked the expression of osteoblast differentiation marker genes including osteocalcin, collagenase-1 and alkaline phosphatase (ALP), and also decreased the expression of osteoclast differentiation genes, such as vacuolar-type H+-ATPase, cathepsin K and receptor activator of NF-κB. Meanwhile bone markers such as serum ALP, osteocalcin (both osteoblast markers) and tartrate-resistant acid phosphatase (an osteoclast marker) were significantly decreased in at least 0·7 % Ca treated groups. These findings indicated that LND diets with ≥0·7 % Ca decreased bone turnover, which subsequently increased tibia quality for 35-d-old meat ducks.

Ração Animal , Remodelação Óssea , Osso e Ossos/efeitos dos fármacos , Cálcio/metabolismo , Suplementos Nutricionais , Tíbia/efeitos dos fármacos , Ciências da Nutrição Animal , Animais , Peso Corporal , Densidade Óssea/efeitos dos fármacos , Patos , Regulação da Expressão Gênica , Masculino , Carne , Osteócitos/metabolismo , Tíbia/fisiopatologia
Br J Nutr ; 115(4): 585-93, 2016 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-26824729


To determine the effects of dietary Fe concentration on Mn bioavailability in rats fed inorganic or organic Mn sources, fifty-four 22-d-old male rats were randomly assigned and fed a basal diet (2·63 mg Fe/kg) supplemented with 0 (low Fe (L-Fe)), 35 (adequate Fe (A-Fe)) or 175 (high Fe (H-Fe)) mg Fe/kg with 10 mg Mn/kg from MnSO4 or Mn-lysine chelate (MnLys). Tissues were harvested after 21 d of feeding. Serum Mn was greater (P<0·05) in MnLys rats than in MnSO4 rats, and in L-Fe rats than in A-Fe or H-Fe rats. Duodenal divalent metal transporter-1 (DMT1) mRNA was lower (P<0·05) in H-Fe rats than in A-Fe rats for the MnSO4 treatment; however, no significant difference was observed between them for MnLys. Liver DMT1 mRNA abundance was greater (P<0·05) in MnSO4 than in the MnLys group for H-Fe rats. The DMT1 protein in duodenum and liver and ferroportin 1 (FPN1) protein in liver was greater (P<0·05) in the MnSO4 group than in the MnLys group, and in L-Fe rats than in H-Fe rats. Duodenal FPN1 protein was greater (P<0·05) in L-Fe rats than in A-Fe rats for the MnLys treatment, but it was not different between them for the MnSO4 treatment. Results suggest that MnLys increased serum Mn concentration as compared with MnSO4 in rats irrespective of dietary Fe concentration, which was not because of the difference in DMT1 and FPN1 expression in the intestine and liver.

Suplementos Nutricionais , Hematínicos/efeitos adversos , Absorção Intestinal , Ferro na Dieta/efeitos adversos , Manganês/administração & dosagem , Animais , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Quelantes/administração & dosagem , Complexos de Coordenação/administração & dosagem , Suplementos Nutricionais/efeitos adversos , Duodeno/metabolismo , Compostos Ferrosos/administração & dosagem , Regulação da Expressão Gênica no Desenvolvimento , Hematínicos/administração & dosagem , Hematínicos/metabolismo , Mucosa Intestinal/metabolismo , Ferro/sangue , Ferro na Dieta/administração & dosagem , Ferro na Dieta/metabolismo , Fígado/metabolismo , Lisina/administração & dosagem , Masculino , Manganês/sangue , Manganês/química , Manganês/metabolismo , Compostos de Manganês/administração & dosagem , Valor Nutritivo , Distribuição Aleatória , Ratos Sprague-Dawley , Sulfatos/administração & dosagem , Desmame