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1.
J Hazard Mater ; 383: 121207, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31539664

RESUMO

China has prohibited an extensive list of solid waste from abroad since 2017. While China seeks to move away from being the world's dumping ground, cleaning up its own backyard is proving to be a great challenge. China's Yangtze River economic zone, which covers 11 provinces and accounts for 40% of the country's Gross Domestic Product, has been found to be alarmingly polluted: 74 million metric tons of solid wastes, including industrial solid waste, construction debris, municipal solid waste, and hazardous waste, have been disposed of by dumping. In this study, the statistics and spatial patterns of waste dumping were determined and mapped, and then the subsequent environmental impacts on the local and downstream marine ecosystem were evaluated. The results indicated the largest dumped-waste volume was found in Sichuan province (industrial solid waste) and Hubei province (solid waste mixture). The potential environmental impacts aroused by waste dumping in Hubei, Jiangxi and Sichuan provinces were serious, while the impacts in Yunnan and Zhejiang were slight. It is imperative for the Yangtze River Economic Zone to develop stringent measures for curbing the dumping of solid waste, assessing the implications from existing dumping activities, and enhancing the capacity for responsible waste management.

2.
Acta Trop ; 201: 105212, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31600521

RESUMO

Fasciolosis is one of the biggest threats to livestock and human population. For this purpose, the seroprevalence of Fasciola hepatica was investigated in yaks and sheep living on the Qinghai-Tibet plateau, China by piloting commercial ELISA kits. A total of 3276 yaks and 1092 sheep were incorporated in this study. The prevalence of the parasite in yaks and sheep was 38.3% and 26.4%, respectively. The serological results revealed a relatively high prevalence of F. hepatica infection in yaks and sheep, respectively. The present study may greatly contribute to the prevention of this parasitic zoonosis and great importance should be given to the potential threat caused by F. hepatica in this special plateau.

3.
Biochim Biophys Acta Mol Basis Dis ; 1866(1): 165554, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31513833

RESUMO

Activation of interferon (IFN)-I signaling in B cells contributes to the pathogenesis of systemic lupus erythematosus (SLE). Recent studies have shown that myeloid-derived suppressor cells (MDSCs) significantly expand in SLE patients and lupus-prone MRL/lpr mice and contribute to the pathogenesis of SLE. However, the role of SLE-derived MDSCs in regulating IFN-I signaling activation of B cells remains unknown. Here, we demonstrate that expansions of MDSCs, including granulocyte (G)-MDSCs and monocytic (M)-MDSCs, during the progression of SLE were correlated with the IFN-I signature of B cells. Interestingly, G-MDSCs from MRL/lpr mice, but not M-MDSCs, could significantly promote IFN-I signaling activation of B cells and contribute to the pathogenesis of SLE. Mechanistically, we identified that the long non-coding RNA NEAT1 was over-expressed in G-MDSCs from MRL/lpr mice and could induce the promotion of G-MDSCs on IFN-I signaling activation of B cells through B cell-activating factor (BAFF) secretion. Importantly, NEAT1 deficiency significantly attenuated the lupus symptoms in pristane-induced lupus mice. In addition, there was a positive correlation between NEAT1 and BAFF with the IFN signature in SLE patients. In conclusion, G-MDSCs may contribute to the IFN signature in SLE B cells through the NEAT1-BAFF axis, highlighting G-MDSCs as a potential therapeutic target to treat SLE.

4.
J Pharm Biomed Anal ; 177: 112855, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31561061

RESUMO

FSH plays a key role in the function of the reproductive system of human beings and is widely used both diagnostically and therapeutically in reproductive medicine. With the growing incidence of infertility, the demand for FSH pharmaceutical products is increasing. For this reason, the quality control process for FSH products is becoming more stringent. An accurate determination of bioactivity is crucial for the safety and efficacy of recombinant human follicle stimulating hormone (rhFSH). Up to now, in-vivo bioassay based on FSH-induced increases in rat ovarian weight has been the only method widely accepted by different pharmacopoeias. However this method has such drawbacks as the complex procedures, long assay period and high variability. Here, we established a reporter gene assay (RGA) based on the CHO-K1-FSHR-CRE-Luc cell line that stably expresses human follicle stimulating hormone receptor (hFSHR), as well as a luciferase reporter under the control of cyclic adenosine monophosphate (cAMP) response elements (CRES). Our study showed that our new assay not only has good dose-dependent responsiveness to rhFSH, but it also performs excellently in terms of specificity, precision, linearity, and simplicity compared with in-vivo rat bioassays. These results implied that this robust reporter gene assay may be a viable supplement to the animal in-vivo bioassay and may be employed in potency determination of rhFSH pharmaceutical products.

5.
Food Chem ; 308: 125599, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31648098

RESUMO

In this work, sequential electrospinning was utilized to fabricate a multilayer film with ethylcellulose nanofibers as the outer layer and curcumin-loaded gelatin nanofibers as the inner layer. Field-emission scanning electronic microscopy observations showed that the outer and inner layers had a smooth surface and clear boundary. The hydrophobic outer layers decreased the water vapor permeability and improved the water contact resistance of the hydrophilic inner layer, and the intimate interactions of hydrogen bonds between two adjacent layers enhanced the thermal stability. The multilayer film exhibited a sustained release manner of the encapsulated curcumin for 96 h, compared to the burst release within 30 min from the gelatin film. In addition, the antioxidant activities of the released curcumin from the multilayer film were well retained within 96 h. These results suggested that the multilayer nanofibrous film fabricated by sequential electrospinning has potential applications in bioactive encapsulation and controlled release.

6.
Talanta ; 207: 120260, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594598

RESUMO

Metabolomics tactics have been applied in the research associated with embryonic zebrafish. However, the report regarding the evaluation of impacts of sample pretreatment on metabolomics results from zebrafish embryos is limited. In the present study, different data normalization approaches, extraction solvents, and extraction strategies for off-line derivatization gas chromatography coupled with mass spectrometry-based metabolomics analysis of zebrafish eleutheroembryos were evaluated and optimized. The results showed that, when 4-chlorophenylalanine normalization, sample homogenization and pure methanol combined with ultrasonic extraction were conducted, better repeatabilities, higher signals and broader coverages of detected metabolites can be achieved. The recovery and standard deviation of most standards were in the range of 82%-121% and 6.6%-12%, respectively, while the relative standard deviation of major detected metabolites ranged from 5.4% to 19%, indicating good extraction efficiencies and method precision. Under the developed method, 87 important endogenous metabolites such as citric acid and hypoxanthine were identified by universal databases or standards among 270 extracted metabolites, which consisted of sugars, amines, amino acids, nucleotides, fatty acids, and sterols. Therefore, the results could provide a proper pretreatment protocol for the analysis of wide-coverage metabolome in embryonic zebrafish. In addition, this study highlights the impact of normalization and extraction methods on the data quality of metabolomics analysis.

7.
Food Microbiol ; 86: 103330, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31703878

RESUMO

Pickle is a type of mildly lactic acid fermented vegetable and is a traditional dish favored in China, Japan, and Korea. Corruption of spoilage bacteria and accumulation of nitrite during vegetable fermentation are common problems that affect the pickle industry and consumer health. In this work, cucumber juice was used as a vegetable model to study the dominant mesophilic aerobic bacteria (MAB) producing nitrite during pickle fermentation. Virulent phages infecting the dominant MABs combined with Lactobacillus plantarum M6 were used to control these bacteria. Enterobacter cloacae and Pseudomonas fluorescens are the dominant MABs in the fermentation of cucumber juice containing 4% or 8% NaCl, with isolation percentages reaching 30.6% and 23.1%, respectively. Virulent phages PspYZU5415 and EcpYZU01 were isolated using P. fluorescens J5415 and E. cloacae J01 as the host bacteria, respectively. These two phages show a broad host range and strong lytic activity, and their genomes contain no toxins and antibiotic resistance genes. PspYZU5415 and EcpYZU01 were combined into a cocktail (designated as Phage MIX) that effectively inhibits the growth of E. cloacae and P. fluorescens in cucumber juice with different salt concentrations. PhageMIX combined with L. plantarum M6 decreased the counts of P. mendocina and E. cloacae to undetectable levels at 48 h during the fermentation of cucumber juice artificially contaminated with P. mendocina and E. cloacae. In addition, nitrite content increased to 11.3 mg/L at 20 h and then degraded completely at 36 h. By contrast, P. mendocina and E. cloacae remained in the groups without PhageMIX during fermentation (0-48 h). Nitrite content rapidly increased to 65.7 mg/L at 12 h and then decreased to 21.6 mg/L at 48 h in the control group. This study suggests that PhageMIX combined with lactic acid bacterial strains can be used as an ecological starter for controlling the dominant MABs P. mendocina and E. cloacae and for reducing nitrate production during the early stage of pickle fermentation.

8.
Chemosphere ; 238: 124611, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31524605

RESUMO

An eco-friendly material, activated carbon cloth (ACC) was used as the heterogeneous catalyst in activation of peroxydisulfate (PDS) for the efficient degradation of organic pollutant in water. Besides, the effects of several parameters in the ACC/PDS process including initial pH, PDS concentration, reaction temperature, stirring speed and co-existing anions were investigated. Under optimum conditions, almost complete removal (98.6%) of AO7 in 60 min and 67.4% of total organic carbon (TOC) removal within 180 min were obtained, accompanied by the remarkable destruction of azo band and naphthalene ring on AO7. The electron paramagnetic resonance and radical quenching experiments were carried out to identify the reactive radicals in the ACC/PDS process. Surface characteristic techniques such as XRD, BET, SEM, FTIR, XPS were applied to analysis the change of crystal structure, surface area, surface morphology, functional groups on the surface of fresh and spent ACC samples. Hydroxyl groups (C‒OH) and π-π transitions significantly affected the catalytic activity of ACC. The intermediate products of AO7 oxidation were identified by LC-MS and the corresponding degradation pathway was proposed.

9.
Chemosphere ; 238: 124863, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31551201

RESUMO

Peripheral blood leukocyte telomere length in omethoate-exposed workers is related to environmental exposure and single nucleotide polymorphisms (SNPs) in genes including p21, GSTM1, miR-145, etc. However, the roles of SNPs in tankyrase (TNKS) gene in telomere length are still unknown. The aim of this study was to explore the association between SNPs in TNKS gene and telomere length in omethoate-exposed workers. Telomere length in peripheral blood leukocyte DNA from 180 omethoate-exposed workers and 115 healthy controls was measured using Real-time quantitative polymerase chain reaction (PCR). Genotyping of the selected functional and susceptible SNPs was performed by the flight mass spectrometry based on PCR and single-base extension. The analysis of covariance was performed to find effects of SNPs on telomere length. Generalized linear models were used to analyze the environment, gene, and interaction on telomere length. The results showed that telomere length in the CG + CC genotypes in rs1055328 in TNKS gene was significantly longer than that in the wild homozygous GG genotype both in exposure group (P = 0.017) and in control group (P = 0.038) after adjusting the covariates. The variables kept in the generalized linear models included omethoate-exposure (ß = 0.580, P = 0.001) and rs1055328 (CG + CC) in TNKS gene (ß = 0.339, P = 0.002). The study suggests that the prolongation of telomere length is associated with omethoate-exposure and the CG + CC genotypes in rs1055328 in TNKS gene.

10.
Water Res ; 168: 115164, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31629229

RESUMO

Carbamazepine (CBZ) is a typical pharmaceutical residue commonly found in aqueous environments, but its removal through activated carbon or advanced oxidation processes is often disrupted by co-existing organic matter. An imprinting system which consisted of the target pollutant CBZ (template molecule) and 10 different kinds of functional monomers was constructed via molecular simulation to screen for appropriate monomers, thereby addressing CBZ removal disruptions. An annealing method simulation was used to search for stable, low-energy conformations of the template-monomer interaction system to calculate the binding energy of these different monomers with CBZ. The order of binding affinity calculated was: 4-vinylbenzoic acid > itaconic acid > methacrylic acid, which was consistent with the experimental observations. The adsorption capacity of the molecular imprinted polymer (MIP) prepared using 4-vinylbenzoic acid reached 28.40 mg/g, and the imprinting factor reached 2.72. The simulation and measurement of the ultraviolet spectrum of the imprinting system showed that a new interaction system was formed between the template and monomers, and that multiple binding conformations between them took place when specific recognition occurred. Energy calculation and hydrogen bond analysis revealed that the van der Waals force, including the π-π conjugate and electrostatic forces including hydrogen bonding, played an important role during selective adsorption, which was confirmed by infrared spectroscopy analysis.


Assuntos
Poluentes Ambientais , Impressão Molecular , Preparações Farmacêuticas , Adsorção , Carbamazepina
11.
IEEE Trans Vis Comput Graph ; 26(1): 407-417, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31442999

RESUMO

Simulative analysis in competitive sports can provide prospective insights, which can help improve the performance of players in future matches. However, adequately simulating the complex competition process and effectively explaining the simulation result to domain experts are typically challenging. This work presents a design study to address these challenges in table tennis. We propose a well-established hybrid second-order Markov chain model to characterize and simulate the competition process in table tennis. Compared with existing methods, our approach is the first to support the effective simulation of tactics, which represent high-level competition strategies in table tennis. Furthermore, we introduce a visual analytics system called Tac-Simur based on the proposed model for simulative visual analytics. Tac-Simur enables users to easily navigate different players and their tactics based on their respective performance in matches to identify the player and the tactics of interest for further analysis. Then, users can utilize the system to interactively explore diverse simulation tasks and visually explain the simulation results. The effectiveness and usefulness of this work are demonstrated by two case studies, in which domain experts utilize Tac-Simur to find interesting and valuable insights. The domain experts also provide positive feedback on the usability of Tac-Simur. Our work can be extended to other similar sports such as tennis and badminton.

12.
Glia ; 68(1): 27-43, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31429156

RESUMO

Ischemic stroke leads to neuronal damage induced by excitotoxicity, inflammation, and oxidative stress. Astrocytes play diverse roles in stroke and ischemia-induced inflammation, and autophagy is critical for maintaining astrocytic functions. Our previous studies showed that the activation of G protein-coupled receptor 30 (GPR30), an estrogen membrane receptor, protected neurons from excitotoxicity. However, the role of astrocytic GPR30 in maintaining autophagy and neuroprotection remained unclear. In this study, we found that the neuroprotection induced by G1 (GPR30 agonist) in wild-type mice after a middle cerebral artery occlusion was completely blocked in GPR30 conventional knockout (KO) mice but partially attenuated in astrocytic or neuronal GPR30 KO mice. In cultured primary astrocytes, glutamate exposure induced astrocyte proliferation and decreased astrocyte autophagy by activating mammalian target of rapamycin (mTOR) and c-Jun N-terminal kinase (JNK) and inhibiting p38 mitogen-activated protein kinase (MAPK) pathway. G1 treatment restored autophagy to its basal level by regulating the p38 pathway but not the mTOR and JNK signaling pathways. Our findings revealed a key role of GPR30 in neuroprotection via the regulation of astrocyte autophagy and support astrocytic GPR30 as a potential drug target against ischemic brain damage.

13.
Oncogene ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31705065

RESUMO

Circular RNAs (circRNAs) have been increasingly indicated to be important participants in the development and progression of various malignant tumors. Our previous studies found that hundreds of circRNAs were aberrantly expressed in bladder cancer (BC) by high-throughput sequencing and we have confirmed that the downregulated circRNAs circHIPK3, circRNA BCRC-3, and circNR3C1 played inhibitory roles in BC progression. In this study, we focused on the upregulated circRNAs and identified a novel circular RNA, hsa_circ_0001361 (circ0001361), was expressed at high levels in BC tissues and cell lines based on RNA-Seq data and qRT-PCR analysis, and it was positively corelated with pathologic grade and muscle invasion. Moreover, Kaplan-Meier survival analysis implied that BC patients with high circ0001361 expression level had a poor overall survival. Functionally, circ0001361 promoted BC cell invasion and metastasis both in vitro and in vivo, but had no effect on cell cycle and proliferation. Mechanistically, RNA sequencing analysis indicated that MMP9 was upregulated in circ0001361-overexpressed BC cells, and MMP9 was verified to mediate circ0001361-induced cell migration and invasion. Furthermore, we demonstrated that circ0001361 could directly interact with miR-491-5p to upregulate MMP9 expression. Collectively, our findings indicate that circ0001361 plays oncogenic role in BC invasion and metastasis through targeting the miR-491-5p/MMP9 axis, and it might be a potential novel target for BC therapy.

14.
Medicine (Baltimore) ; 98(46): e18042, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31725681

RESUMO

BACKGROUND: An increasing number of studies indicate that circular RNAs (circRNAs) participate in tumorigenesis. The aim of this study was to elucidate the regulatory mechanisms of circRNAs in breast cancer based on the construction of the circRNA-related ceRNA network. METHODS: The expression profiles of circRNAs, miRNAs, and mRNAs were obtained from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. A ceRNA network was constructed by Cytoscape. The interactions among proteins were analyzed using the STRING database, and hub genes were extracted using the cytoHubba application. The functions of the differentially expressed mRNAs (DEmRNAs) were analyzed by the Kyoto Encyclopedia of Gene and Genomes (KEGG) and the Gene Ontology (GO) database. RESULTS: In total, 7 differentially expressed circRNAs (DEcircRNAs), 27 differentially expressed miRNAs (DEmiRNAs), and 102 DEmRNAs were selected for the construction of the ceRNA network of breast cancer. We established a protein-protein interaction network and identified 6 hub genes. Then, a circRNA-miRNA-hub gene regulatory module was established based on 2 DEcircRNAs, 2 DEmiRNAs, and 2 DEmRNAs. GO and KEGG pathway analyses indicated the possible association of DEmRNAs with breast cancer onset and progression. CONCLUSIONS: The circRNA hsa_circ_0000519 is likely critical in the pathogenesis of breast cancer and may serve as a future therapeutic biomarker.

15.
Int J Biol Macromol ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31726120

RESUMO

Leaching and re-absorption of leached solids and changes in kernel structure are of prime importance for the quality of cooked rice. This paper describes the contents and structural changes of both starch and protein in leached solids and rice kernels. Under actual cooking conditions, the leached solids increased with heating temperature and then decreased at specific temperatures relating gelatinization. Starch content of leached solids increased with the heating temperature, especially after gelatinization. As proteins are mainly distributed on the surface of the kernel, their release increased between 30 and 50 °C but then decreased with increasing leaching of starch at high temperatures. The molecular weight distribution of leached solids at different temperatures is significantly different, while it is similar in rice kernels. At 30-50 °C, the amylose contents in the leached solids were slightly higher than those in rice kernel, while the contents were similar at high temperatures. The transformation of the kernel structure during heating confirmed that the kernel surface and the formed cracks are the main path for leaching; the rearranged structures of the surface and cracks influence the final texture of cooked rice.

16.
Food Sci Technol Int ; : 1082013219883054, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31726872

RESUMO

The purpose of this study is to reveal the mechanism of preparing high quality modified starch by ultrasonic technology. In this paper, ultrasonic modified starch and octenyl succinic anhydride modified starch with low degree of substitution were prepared under ultrasonic conditions, using sweet potato starch as raw material. The effects of ultrasound on the structure and properties of native sweet potato starch were studied to see whether ultrasound could produce mechanochemical effect on starch. Then the mechanism of ultrasonic effect on quality of octenyl succinic anhydride modified starch was studied by mechanochemical effect. The results showed that after ultrasonic treatment for 1 min, the crystallinity decreased from 37.6 to 33.8% and reaction efficiency increased from 49.43 to 54.39%, while after ultrasonic treatments for 8 and 32-60 min had different changes. These changes showed that ultrasonic treatment produced significant mechanochemical effect on native sweet potato starch. Ultrasound significantly improved the quality of octenyl succinic anhydride modified starch, and its influence mechanism was revealed using the theory of mechanochemistry. This study provides a feasible method for the research of high quality modified starch and lays a theoretical foundation for expanding the application of ultrasound in various fields.

17.
Int Immunopharmacol ; : 105934, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31727560

RESUMO

BACKGROUNDS: C1q tumor necrosis factor-related protein 9 (CTRP9) has been suggested to exert an atheroprotective effect by modulating the inflammation, foam cell formation, endothelia and smooth muscle cell function via Adenosine Monophosphate Activated Protein Kinase (AMPK) pathway. On the other hand, the NLR Family Pyrin Domain Containing 3 (NLRP3) inflammasome plays an critical role in the atherosclerosis development, which is regulated by the AMPK. However, whether the CTRP9 affects the activity of NLRP3 inflammasome during the atherosclerosis development remains unclear, which would be elucidated in the current study. METHODS: The macrophage cells were stimulated with the oxidized low-density lipoprotein (ox-LDL) and also treated with the recombinant CTRP9 in the meantime. The activation of NLRP3 inflammasome was determined by measuring the releasing of IL-1ß and caspase-1 p10 via ELISA and western blot, respectively. Then the AMPK was inhibited in macrophages by Dorsomorphin. Finally, the CTRP9-AMPK-NLRP3 inflammasome pathway was validated in the mouse model of atherosclerosis. RESULTS: The CTRP9 could down-regulate the expression of NLRP3 protein and also the activity of NLRP3 inflammasome in the ox-LDL activated macrophages. Inhibiting the AMPK significantly restored the activities of NLRP3 inflammasome. In the apolipoprotein E-deficient mice, lentiviral expression of CTRP9 could suppress the atherosclerosis development, which could be abolished by AMPK inhibition. CONCLUSION: Our data here indicated that the CTRP9 showed atheroprotective function via CTRP9-AMPK- NLRP3 inflammasome pathway.

18.
BMJ Open ; 9(11): e031798, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727657

RESUMO

OBJECTIVES: We aimed to analyse the healthcare seeking behaviours (HSB) of populations with different migration status and examine the relationship between migration status and HSB. DESIGN: A secondary analysis of cross-sectional data of China Labor-force Dynamics Survey wave 2016, which was conducted by the Sun Yat-sen University every 2 years since 2010. SETTING: 29 provinces/cities/autonomous regions in mainland China, excluding Tibet and Hainan province. PARTICIPANTS: 3522 respondents in a condition in the previous 2 weeks or during the last 12 months were analysed. MAIN OUTCOME MEASURES: The migration status included internal migrants (IMs), returned population, rural residents and urban residents. The HSB including health services utilisation and choice of health facilities for their health services. Multivariable logistic regressions were used to examine the association between migration status and HSB. RESULTS: 2193 and 1898 of 3522 respondents were in need of outpatient services (having a condition in the previous 2 weeks) or inpatient services (being asked to use the inpatient services during the last 12 months). Compared with urban residents, the returned population (OR=0.66, 95% CIs 0.49 to 0.89) and rural residents (OR=0.65, 95% CIs 0.51 to 0.82) were less likely to have an unmet need for outpatient services. The latter tended to choose lower-level hospitals, while the IMs preferred the lowest-level healthcare facilities for health services. After controlling for confounding factors, the results above did not reach any statistical significance. CONCLUSION: Association between migration status and HSB might be through enabling resource and needs. Returned population, IMs and rural residents tended to choose low-level hospitals for their health service needs, but this association was mainly associated with their poorer social and economic resources. Improving their social and economic resources would be helpful for reducing the barriers of HSB.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31670674

RESUMO

Small object arrangement is very important for creating detailed and realistic 3D indoor scenes. In this paper, we present an interactive framework based on active learning to help users create customized arrangements for small objects according to their preferences. To achieve this with minimal user effort, we first learn the prior knowledge about small object arrangement from a 3D indoor scene dataset through a probability mining method, which forms the initial guidance for arranging small objects. Then, users are able to express their preferences on a few small object categories, which are automatically propagated to all the other categories via a novel active learning approach.In the propagation process, we introduce a novel metric to obtain the propagation weights, which measures the degree of interchangeability between two small object categories, and is calculated based on a spatial embedding model learned from the small object neighborhood information extracted from the 3D indoor scene dataset. Experiments show that our framework is able to help users effectively create customized small object arrangements with little effort.

20.
J Agric Food Chem ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31674784

RESUMO

l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties. In this study, we aim to investigate whether l-arabinose could inhibit colitis by modulating gut microbiota. l-Arabinose was administered in mice daily in a dextran sodium sulfate (DSS)-induced colitis model. The histological analysis, disease index, and the expression of inflammatory genes were measured. 16S-rRNA sequence analysis was performed to investigate gut microbiota. Intriguingly, we found that l-arabinose could repress DSS-induced colitis and inhibit p38-/p65-dependent inflammation activation. Besides that, our data revealed that l-arabinose-modulated DSS-induced gut microbiota were disturbed. Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics. Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease. As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha. Our work suggested that l-arabinose exerts anti-inflammation effects in DSS-induced colitis. These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose. l-Arabinose could be a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health.

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