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1.
Sci Total Environ ; 701: 134772, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31731204

RESUMO

Monolayer graphene film (Gr) as a metal-free catalyst was synthesized on the paper-like sintered stainless steel fibers (PSSF) with three-dimensional net structure by chemical vapor deposition (CVD) technique. The prepared PSSF-Gr was characterized by SEM, EDS, XRD, AFM, TEM, and Raman spectroscopy. Then, the optimum reaction conditions for catalytic wet peroxide oxidation (CWPO) of phenol in a continuous reactor using PSSF-Gr catalysts were explored by analyzing the effects of reaction temperature, feed flow rate, and catalyst bed height on catalytic performance. Moreover, the long-term stability of PSSF-Gr catalyst was investigated and demonstrated complete phenol oxidation and dramatic TOC removal (values ranging between 80.7% and 91.0%) after continuously operating for 72 h under optimum condition. Finally, a reasonable reaction mechanism for CWPO of phenol was proposed by analyzing the HPLC results and evolution of aromatic intermediates content. From these results, seldom toxic aromatic intermediates were observed on account of the production of short-chain organic acids by opening of aromatic ring, which subsequently mineralized to CO2 and H2O. The simple preparation method, unique structure, extraordinary catalytic activity and stability of the graphene-based material would provide a new potential catalyst for environmental catalysis.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31786691

RESUMO

Schizophrenia is a psychiatric condition characterized by poor prognosis and severe symptoms that decrease the quality of life of patients. It is therefore important to develop rapid and reliable methods for early diagnosis. To achieve this aim, identification of accurate biomarkers will promote research into the mechanisms of schizophrenia and the design of effective diagnosis tools. Discriminative peptides in the serum of participants (277 schizophrenia patients and 294 healthy controls) were detected using the matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS). The diagnostic model for schizophrenia was validated using the ClinProTool software. Discrimination models were tested in the training set (200 schizophrenia patients and 200 healthy controls), and the robustness of the models was evaluated using the independent test set (77 cases and 94 controls). A total of 77 peaks were significantly different between schizophrenia patients and healthy controls with a signal-to-noise ratio of over 5. Among them, 35 peptides were down-regulated and 42 peptides were up-regulated in schizophrenia patients. With a cross-validation rate of 92.7% and a recognition capability rate of 96.5%, the supervised neural network comprising 25 discriminative peaks was found to be the most efficient model for schizophrenia diagnosis (sensitivity = 96.10%, specificity = 94.68%). Peptides at m/z = 2022.18 corresponded to complement C3f, and peptides at m/z = 1020.89, 1351.02, 1466.1 were identified as fragments of fibrinopeptide A. These two peptides may be potential biomarkers for schizophrenia in the Chinese population. The serum peptide levels present a potential clinical diagnostic tool for schizophrenia.

3.
Sci Rep ; 9(1): 18774, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31801960

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

4.
J Mol Cell Cardiol ; 138: 34-48, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31733201

RESUMO

Homocysteine (Hcy) is an independent risk factor for atherosclerosis, which is characterized by lipid accumulation in the atherosclerotic plaque. Increasing evidence supports that as the main receptor of high-density lipoprotein, scavenger receptor class B member 1 (SCARB1) is protective against atherosclerosis. However, the underlying mechanism regarding it in Hcy-mediated atherosclerosis remains unclear. Here, we found the remarkable inhibition of SCARB1 expression in atherosclerotic plaque and Hcy-treated foam cells, whereas overexpression of SCARB1 can suppress lipid accumulation in foam cells following Hcy treatment. Analysis of SCARB1 promoter showed that no significant change of methylation level was observed both in vivo and in vitro under Hcy treatment. Moreover, it was found that the negative regulation of DNMT3b on SCARB1 was due to the decreased recruitment of SP1 to SCARB1 promoter. Thus, we concluded that inhibition of SCARB1 expression induced by DNMT3b at least partly accelerated Hcy-mediated atherosclerosis through promoting lipid accumulation in foam cells, which was attributed to the decreased binding of SP1 to SCARB1 promoter. In our point, these findings will provide novel insight into an epigenetic mechanism for atherosclerosis.

5.
J Affect Disord ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31780131

RESUMO

BACKGROUND: The present study aimed to determine whether there were gender differences in the effects of prenatal maternal depression on motor development in newborn infants, and further to explore the role of plasma homocysteine in the delayed motor development in male newborn infants following prenatal maternal depression. METHODS: The term pregnant women within 37-42 weeks of gestation were assessed depressive symptoms by Hamilton Rating Scale for Depression. According to the gender of the newborn infants, all the subjects were divided into four groups: female control group (n = 45), male control group (n = 47), female depression group (n = 50), male depression group (n = 60). Motor development in newborn infants were assessed by Neonatal Behavioral Assessment Scale. Plasma homocysteine concentrations both in mothers and newborn infants were measured by enzymatic cycling assay. RESULTS: There were the worse scores of the items of motor development and significantly higher plasma homocysteine concentrations  in the male newborn infants of depression group than those of the female depression group and female control group, male control group, respectively. Plasma homocysteine concentrations significantly correlated with the items of motor development in all newborn infants, including the depression group and control group. LIMITATIONS: We should further explore homocysteine-mediated gender-dependent effects of prenatal maternal depression on motor development in newborn infants in the long-term follow-up. CONCLUSIONS: Prenatal maternal depression could result in delayed motor development in male newborn infants, but not female newborn infants. Plasma homocysteine may mediate gender-dependent effects of prenatal maternal depression on motor development in newborn infants.

6.
Asian J Androl ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31744994

RESUMO

Varicocele is one of the most important causes of male infertility, as this condition leads to a decline in sperm quality. It is generally believed that the presence of varicocele induces an increase in reactive oxygen species levels, leading to oxidative stress and sperm apoptosis; however, the specific pathogenic mechanisms affecting spermatogenesis remain elusive. Prokineticin 2 (PK2), a secretory protein, is associated with multiple biological processes, including cell migration, proliferation, and apoptosis. In the testis, PK2 is expressed in spermatocytes under normal physiological conditions. To investigate the role of PK2 in varicocele, a rat varicocele model was established to locate and quantify the expression of PK2 and its receptor, prokineticin receptor 1 (PKR1), by immunohistochemistry and quantitative real-time PCR assays (qPCR). Moreover, H2O2 was applied to mimic the oxidative stress state of varicocele through coculturing with a spermatocyte-derived cell line (GC-2) in vitro, and the apoptosis rate was detected by flow cytometry. Here, we illustrated that the expression levels of PK2 and PKR1 were upregulated in the spermatocytes of the rat model. Administration of H2O2 stimulated the overexpression of PK2 in GC-2. Transfection of recombinant pCMV-HA-PK2 into GC-2 cells promoted apoptosis by upregulating cleaved-caspase-3, caspase-8, and B cell lymphoma 2-associated X; downregulating B cell lymphoma 2; and promoting the accumulation of intracellular calcium. Overall, we revealed that the varicocele-induced oxidative stress stimulated the overexpression of PK2, leading to apoptosis of spermatocytes. Our study provides new insight into the mechanisms underlying oxidative stress-associated male infertility and suggests a novel therapeutic target for male infertility.

7.
J Hazard Mater ; 384: 121246, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31585277

RESUMO

Different layers of graphene (Gr) films are prepared on the paper-like sintered stainless steel fibers (PSSF) support with three-dimensional structure by CVD method. The effects of acetylene flow rate, deposition time, and deposition temperature on the properties of PSSF-Gr are investigated by EDS, AFM, SEM, TEM, and Raman spectroscopy, respectively. Then, the catalytic performances of PSSF-Gr with different layers of Gr films as metal-free catalysts for catalytic wet peroxide oxidation (CWPO) of phenol are assessed in the continuous fixed-bed reactor. The catalytic results demonstrate that the PSSF-Gr catalyst with single layer graphene film achieves the best catalytic performance (phenol and TOC removal efficiency reach 99% and 73%, respectively) after continuously operating for 6 h. Under the treatment of the PSSF-Gr catalyst with single-layer graphene, total phenol oxidation and excellent TOC removal (maintain about 71%) have been achieved for the long-term operation (38 h). Moreover, the phenol conversion of blank experiment (without catalyst) and PSSF are around 40%, which are caused by thermal degradation and thus, the excellent catalytic activity of PSSF-Gr is ascribed to graphene. Like other Fenton's catalysts, the catalytic mechanism of PSSF-Gr catalyst in phenol degradation is also a ·OH mechanism.

8.
Violence Vict ; 34(5): 838-849, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31575818

RESUMO

Intimate partner violence is an important public health issue affecting the well-being of the young adults. However, there is little epidemiological evidence on the incidence and associated mental health problems of both intimate partner violence perpetration and victimization in Chinese context. Using one representative community sample of 1,227 young adults aged 18-27 years in 2016, this study aimed to examine the prevalence, sociodemographic characteristics, and possible mental health consequences of intimate partner violence among young adults in Hong Kong. It is found that the prevalence of preceding 1-year intimate partner violence perpetration and victimization was 5.3% and 9.1% respectively. The perpetrators were worse educated, unemployed, and married, while the victims were also unemployed and cohabited. Compared with those without involving in any intimate partner violence, perpetrators of intimate partner violence were more likely to engage in frequent smoking and alcohol use, and victims of intimate partner violence reported lower life satisfaction, more self-harm behaviors, higher suicide ideation and attempt, and heavy alcohol use. Both perpetrators and victims were more likely to report the worst mental health problems and high risky behaviors.

9.
Front Immunol ; 10: 1872, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31474981

RESUMO

Infections of the reproductive tract are known to contribute to testicular inflammatory impairment, leading to an increase of pro-inflammatory cytokines such as IL-1ß, and a decline in sperm quality. Prokineticin 2 (PK2), a secretory protein, is closely associated with the secretion of pro-inflammatory cytokines in inflamed tissue. It was reported that increased PK2 is related to the upregulation of IL-1ß, but the underlying mechanism remains elusive. Here, we illustrated that PK2 was upregulated in testicular macrophages (TM) in a rat model of uropathogenic Escherichia coli (UPEC) infection, which induced the activation of the NLRP3 inflammasome pathway to boost IL-1ß secretion. Administration of PK2 inhibitor alleviated the inflammatory damage and suppressed IL-1ß secretion. Moreover, PK2 promoted NLRP3 expression and the release of cleaved IL-1ß from TM to the supernatants after the challenge with UPEC in vitro. IL-1ß in the supernatants affected Leydig cells by suppressing the expression of genes encoding for the enzymes P450scc and P450c17, which are involved in testosterone production. Overall, we revealed that increased PK2 levels in TM in UPEC-induced orchitis may impair testosterone synthesis via the activation of the NLRP3 pathway. Our study provides a new insight into the mechanisms underlying inflammation-associated male infertility and suggests an anti-inflammatory therapeutic target for male infertility.

10.
Chem Pharm Bull (Tokyo) ; 67(8): 775-777, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31366826

RESUMO

Nocardia is a potent bacterial producer of bioactive compounds. From a culture of Nocardia beijingensis NBRC 16342, we isolated four aromatic compounds, named beijinchromes A-D (1-4). We purified them by silica gel chromatography and reverse phase HPLC, and identified their structures by NMR and high resolution (HR)-MS analyses. 1, 2, and 4 are novel 1,2,3,8-tetrasubstituted naphthalenes, and 3 is a novel 3,8-disubstituted ortho-naphthoquinone. 1 and 2 exert antioxidant activities, and 3 exhibits antibiotic activity. Remarkably, the putative biosynthetic gene clusters for 1-4 are widely distributed in 37 Nocardia species, implying their potential to produce this family of compounds and important biological functions of beijinchromes.


Assuntos
Naftalenos/química , Naftoquinonas/química , Nocardia/química , Estrutura Molecular , Naftalenos/isolamento & purificação , Naftalenos/farmacologia , Naftoquinonas/isolamento & purificação , Naftoquinonas/farmacologia , Estereoisomerismo
11.
Neuroscience ; 414: 186-199, 2019 08 21.
Artigo em Inglês | MEDLINE | ID: mdl-31283971

RESUMO

Substantial evidence has demonstrated that prenatal stress (PS) impairs spatial learning and memory in offspring. The neuron-specific protein kinase C gamma (PKCγ) has been proposed to be unique in spatial learning and memory. The present study proposes to determine whether hippocampal PKCγ is involved in the detrimental effects of PS on spatial learning and memory in offspring, and to further explore the effects of PS-induced PKCγ-dependent growth-associated protein 43 (GAP-43) and neurogranin (Ng) phosphorylation alteration on calcium/calmodulin-dependent protein kinase II (CaMKII) activation. Prenatal restraint stress models were established, and lentivirus-mediated overexpression of PKCγ in the hippocampal CA1 area was applied. The results demonstrated that PS impaired spatial learning acquisition and memory retrieval on the Morris Water Maze test, especially in juvenile female rats. Hippocampal PKCγ membrane translocation and cytosolic PKCγ levels were decreased in PS females. The expression of phosphorylated GAP-43 (p-GAP-43) and phosphorylated Ng (p-Ng), as well as phosphorylated CaMKII (p-CaMKII), was significantly reduced in the hippocampus of PS females. Overexpression of PKCγ in the hippocampal CA1 area recovered the ability of spatial learning and memory in PS female offspring. Furthermore, enhancing PKCγ reversed PS-induced membrane and cytosolic PKCγ reduction, and restored levels of GAP-43 and Ng phosphorylation, and CaMKII activation in the hippocampus. In conclusion, PS possibly decreases hippocampal PKCγ activity, resulting in a reduction of p-GAP-43 and p-Ng, which underlies insufficient CaMKII activation, thereby impairing spatial learning and memory.

12.
J Affect Disord ; 257: 241-249, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31301627

RESUMO

BACKGROUND: Increased cortisol has been shown to be negatively correlated with infant motor development. Sunlight help decrease the level of cortisol. Vitamin D is associated with infant motor development. The present study aimed to determine whether natural sunlight exposure plus vitamin D supplements could ameliorate delayed early motor development in little infants from maternal perinatal depression. METHODS: The term pregnant women waiting for delivery from the department of gynecology and obstetrics were assessed depressive symptoms by Hamilton Rating Scale for Depression (HAMD). 120 normal and 229 depressed subjects were recruited. During 2 days postpartum, infant motor development were assessed by Neonatal Behavioral Assessment Scale (NBAS). Infants of 2-day-old in maternal depression group were divided into four groups: control group, conventional vitamin D supplements (400IU/d) group, high dose of vitamin D supplements group (1000IU/d), sunlight plus conventional vitamin D supplement group (400IU/d). Serum and hair cortisol (HairF) in mothers and infants were measured. RESULTS: The infants of perinatal depressed mothers displayed early motor developmental delay accompanied by increased cortisol. Sunlight plus conventional vitamin D supplement (400IU/d) were better than exclusive vitamin D supplements for the amelioration delayed early motor development in infants (p < 0.05). The infants exposure to sunlight 7-14 h/week plus conventional vitamin D supplement reached the best scores of motor development and the lowest HairF (p < 0.05). LIMITATIONS: We should have measured the serum 25OH-vitamin D concentrations. CONCLUSIONS: Sunlight plus vitamin D supplements could ameliorate delayed early motor development in little infants by decreasing cortisol from perinatal depression.

13.
Child Abuse Negl ; 96: 104072, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31319239

RESUMO

BACKGROUND: Bullying victimization among school-aged children is an important public health issue that may affect their well-being and mental health. However, few studies have been conducted on left-behind children in rural China, who are defined as the children who stay in rural areas for more than six months and have one or both parents migrating to urban areas for work. The mechanisms through which bullying victimization will influence depression have disproportionally adopted a psychopathological perspective, and the protective factors are understudied. OBJECTIVE: This study aims at investigating the factors that might contribute to breaking up the vicious circle between bullying victimization and developmental problems, focusing on the protective role of self-compassion and hope in the association between bullying victimization and depression. METHODS: Using questionnaires, data were collected from a sample of 1091 school-aged left-behind children from west and central China. RESULTS: Compared with rural children living with their parents, left-behind children reported a higher level of bullying victimization. Bullying victimization was positively associated with depression through decreased self-compassion and hope, and self- compassion played a more crucial role than hope in the linkage between bullying victimization and depression. CONCLUSIONS: Our study's findings suggest that it is necessary to incorporate self-compassion and hope in mental health prevention and intervention programs targeting left-behind children with bullying victimization experiences.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31233264

RESUMO

OBJECTIVE: Little is known about how metabolic perturbations are linked to hyperuricemia in the general population. Therefore we aimed to examine metabolomics profiles in relation to uric acid change and incident hyperuricemia. METHODS: This study included 1,621 community-dwelling Chinese participants aged 50-70 years without hyperuricemia at baseline, with mean duration of follow-up 6 years. A total of 56 metabolites (22 amino acids and 34 acylcarnitines) at baseline were quantified by gas or liquid chromatography coupled to mass spectrometry. Annual change in uric acid was calculated, and incident hyperuricemia was defined as plasma uric acid >420 µmol/L in men and >360 µmol/L in women. RESULTS: The mean (SD) annual change in uric acid was 9.6 ± 12.1 µmol/L and the incidence of hyperuricemia was 23.1% (375/1,621). After adjustment for conventional risk factors, 9 metabolites (cysteine, glutamine, phenylalanine, threonine, and long-chain acylcarnitines C14:1OH, C18, C18:2, C20, and C20:4) were significantly associated with uric acid change (Bonferroni corrected P <0.05) and incident hyperuricemia (relative risks ranged from 1.14-1.21 per SD increment of metabolites; P <0.05). A network analysis showed significant associations between the module containing long-chain acylcarnitines and incident hyperuricemia. Moreover, levels of these 9 metabolites were specifically correlated with intake of foods including red and processed meat or soy products. CONCLUSIONS: Plasma cysteine, glutamine, phenylalanine, threonine, and long-chain acylcarnitines are positively associated with incident hyperuricemia. The levels of these metabolites may be partially driven by intakes of meat and soy products that are associated with hyperuricemia. This article is protected by copyright. All rights reserved.

15.
Org Lett ; 21(12): 4788-4792, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31180682

RESUMO

Heterologous expression of a unique biosynthetic gene cluster (BGC) comprising a highly reducing polyketide synthase and stand-alone thioesterase genes in Aspergillus oryzae enabled us to isolate a novel 34-membered polyene macrolide, phaeospelide A (1). This is the first isolation of a fungal polyene macrolide and the first demonstration of fungal aliphatic macrolide biosynthetic machinery. In addition, sequence similarity network analysis demonstrated the existence of a large number of BGCs for novel fungal macrolides.

16.
Epigenomics ; 11(7): 739-749, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31140863

RESUMO

Aim: Salivary miRNA can be easily accessible biomarkers of alcohol dependence (AD). Materials & methods: The miRNA transcriptome in the saliva of 56 African-Americans (AAs; 28 AD patients/28 controls) and 64 European-Americans (EAs; 32 AD patients/32 controls) was profiled using small RNA sequencing. Differentially expressed miRNAs were identified. Salivary miRNAs were used to predict the AD presence using machine learning with Random Forests. Results: Seven miRNAs were differentially expressed in AA AD patients, and five miRNAs were differentially expressed in EA AD patients. The AD prediction accuracy based on top five miRNAs (ranked by Gini index) was 79.1 and 72.2% in AAs and EAs, respectively. Conclusion: This study provided the first evidence that salivary miRNAs are AD biomarkers.

17.
J Interpers Violence ; : 886260519843287, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30983481

RESUMO

Bullying victimization among Chinese school-aged children has recently been recognized as a serious problem. However, studies on the prevalence and correlates of bullying victimization among left-behind children-whose parents live and work in urban areas while they remain at home in a rural area-have fallen far behind. We conducted a study based on one representative sample of 742 left-behind children in Grades 6 to 10 from rural China in 2010. The results indicate that a total of 31.6% of the left-behind children report recurrent bullying victimization, which is higher than that of their rural counterparts who live with their parents. Furthermore, left-behind children who experience bullying victimization are more likely to report being drunk frequently, poorer peer and family relationships, and academic problems. Future bullying prevention and intervention programs should be designed to meet the special needs of left-behind children.

18.
Sci Rep ; 9(1): 4660, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30874594

RESUMO

There is currently an epidemic of opioid use, overdose, and dependence in the United States. Although opioid dependence (OD) is more prevalent in men, opioid relapse and fatal opioid overdoses have recently increased at a higher rate among women. Epigenetic mechanisms have been implicated in the etiology of OD, though most studies to date have used candidate gene approaches. We conducted the first epigenome-wide association study (EWAS) of OD in a sample of 220 European-American (EA) women (140 OD cases, 80 opioid-exposed controls). DNA was derived from whole blood samples and EWAS was implemented using the Illumina Infinium HumanMethylationEPIC array. To identify differentially methylated CpG sites, we performed an association analysis adjusting for age, estimates of cell proportions, smoking status, and the first three principal components to correct for population stratification. After correction for multiple testing, association analysis identified three genome-wide significant differentially methylated CpG sites mapping to the PARG, RERE, and CFAP77 genes. These genes are involved in chromatin remodeling, DNA binding, cell survival, and cell projection. Previous genome-wide association studies have identified RERE risk variants in association with psychiatric disorders and educational attainment. DNA methylation age in the peripheral blood did not differ between OD subjects and opioid-exposed controls. Our findings implicate epigenetic mechanisms in OD and, if replicated, identify possible novel peripheral biomarkers of OD that could inform the prevention and treatment of the disorder.

19.
Biosci Rep ; 39(3)2019 03 29.
Artigo em Inglês | MEDLINE | ID: mdl-30765615

RESUMO

Background. The results of previous studies on the association between polymorphisms of CYP1A1 and CYP1B1 and prostate cancer (PCa) susceptibility are inconsistent. The aim of the present study was to conduct a meta-analysis in order to better estimate this association. Methods. A systematic search was carried out on PubMed, Embase, Cochrane Library, and China National Knowledge Infrastructure (CNKI) databases for relevant articles published up to 15 August 2018. Pooled odds ratios (ORs) and 95% confidence intervals were obtained using fixed-effect or random-effect models. Results. A significant association was found between the CYP1A1 rs1048943 polymorphism and PCa in the overall population (B [the minor allele] vs. A [the major allele]: OR = 1.20, 95% confidence interval (CI) = 1.04-1.39, P=0.014; AB vs. AA: OR = 1.24, 95% CI = 1.02-1.51, P=0.029; BB + AB vs. AA: OR = 1.25, 95% CI = 1.04-1.50, P=0.018) and Asian population (B vs. A: OR = 1.32, 95% CI = 1.11-1.56, P=0.001; BB vs. AA: OR = 1.81, 95% CI = 1.20-2.72, P=0.005; AB vs. AA: OR = 1.30, 95% CI = 1.03-1.64, P=0.029; BB + AB vs. AA: OR = 1.38, 95% CI = 1.11-1.73, P=0.004; BB vs. AA + AB: OR = 1.58, 95% CI = 1.08-2.01, P=0.019), but not in the Caucasian population. Moreover, we found that the rs4646903 polymorphism was associated with a significant increase in the risk of PCa in the Asian population (AB vs. AA: OR = 1.43, 95% CI = 1.13-1.80, P=0.003) and Caucasian population (BB vs. AA: OR = 2.12, 95% CI = 1.29-3.49, P=0.003). Conclusion. This meta-analysis revealed a clear association between rs1048943 and rs4646903 polymorphisms of the CYP1A1 gene but not between CYP1B1 rs10012, rs162549, rs1800440, and rs2551188 polymorphisms and the risk of PCa.

20.
Fitoterapia ; 133: 35-42, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30572089

RESUMO

Three new lignoids, premnan A (1), premnan B (2), and tauntangyiol C (3), were isolated from Premna serratifolia wood, a traditional cosmetic plant in Myanmar, together with a new lignoid, premnan C (4) assumed to be an artifact, one natural new lignoid (5), and three known lignoids (6-8). The structures of the new compounds 1-4 were elucidated based on 1D and 2D NMR, IR spectroscopy, and HRESIMS. The absolute configurations of 1-4 were also determined by optical rotation, circular dichroism (CD) data analyses, and comparisons with the reported literature. All isolated compounds were tested for their melanogenesis activities against the B16-F10 mouse melanoma cell line. Compounds 1 and 4 showed melanogenesis enhancing activities of 31% and 50%, respectively, at a 50 µM concentration. Compounds 2, 3, and 6 increased melanin production by 67%, 30%, and 45%, respectively, at a 100 µM concentration, without any cytotoxicity.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Lamiaceae/química , Lignanas/farmacologia , Madeira/química , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Lignanas/isolamento & purificação , Melaninas , Melanoma Experimental , Camundongos , Estrutura Molecular , Mianmar , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
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