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1.
Oncogene ; 2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33824474

RESUMO

Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal malignancies and rapidly progressive diseases. Exosomes and long noncoding RNAs (lncRNAs) are emerging as vital mediators in tumor cells and their microenvironment. However, the detailed roles and mechanisms of exosomal lncRNAs in PDAC progression remain unknown. Here, we aimed to clarify the clinical significance and mechanisms of exosomal lncRNA 01133 (LINC01133) in PDAC. We analyzed the expression of LINC01133 in PDAC and found that exosomal LINC01133 expression was high and positively correlated with higher TNM stage and poor overall survival rate of PDAC patients. Further research demonstrated that Periostin could increase exosome secretion and then enhance LINC01133 expression. In addition, Periostin increased p-EGFR, p-Erk, and c-myc expression, and c-myc could bind to the LINC01133 promoter region. These findings suggested that LINC01133 can be regulated by Periostin via EGFR pathway activity. We also observed that LINC01133 promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of pancreatic cancer cells. We subsequently evaluated the effect of LINC01133 on the Wnt/ß-catenin pathway and confirmed that LINC01133 can interact with Enhancer Of Zeste Homolog 2 (EZH2) and then promote H3K27 trimethylation. This can further silence AXIN2 and suppress GSK3 activity, ultimately activating ß-catenin. Collectively, these data indicate that exosomal LINC01133 plays an important role in pancreatic tumor progression, and targeting LINC01133 may provide a potential treatment strategy for PDAC.

2.
Chemosphere ; 278: 130349, 2021 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-33838424

RESUMO

Triclocarban (TCC), considered an endocrine-disrupting, persistent, and bioaccumulating organic matter, has attracted a great deal of attention for its pollution and health risks. However, studies on its toxicological mechanism, especially for embryo development are limited. This article explores the cardiac developmental toxicity induced in zebrafish embryos after exposure to different TCC concentrations. First, liquid chromatography-tandem mass spectrometry was used in detecting TCC in embryos in vivo after exposure to various TCC. Results showed that embryonic TCC content reached 9.23 ng after exposure to 300 µg/L TCC, the heart rates of the embryos markedly decreased, heart abnormalities significantly increased. In addition, obvious pericardial effusion was observed in the larvae. Through transcriptome sequencing, 200 differential gene expression (DGE) patterns were detected in the TCC (300 µg/L) experimental and control groups. The results of GO function analysis and KEGG pathway of DGE showed that aryl hydrocarbon receptor (AhR) activation and cyp-related genes (cyp1a, cyp1b1 and cyp1c) were significantly up-regulated. these affected the normal development of zebrafish embryonic heart, tissue edema, and hemorrhage. TCC exhibited strong cardiac teratogenic effects and developmental toxicity, which is partly related to AhR activation. Transcriptome-based results are helpful in precisely determining the risk of TCC exposure. The potential mechanism between TCC and AhR should be further investigated.

3.
Artigo em Inglês | MEDLINE | ID: mdl-33837566

RESUMO

OBJECTIVE: Effective family planning services (FPs) use significantly improves both women and men sexual and reproductive health. Recently, the Chinese government has launched the two-child policy allowing families to have additional child. This study aimed to explore the population interest for FPs, unmet needs and the populations' expectations. METHODS: Cross-sectional study using stratified random sampling was conducted in 102 counties of Hubei province and 17,555 randomized subjects from rural, transitional, and urban areas were interviewed through a guided semi-structured questionnaire. RESULTS: The Han ethnicity dominated our population and the living children per family ranged 0-6. Although 81% reported family planning services and sexual and reproductive health (FPs/SRH) as a shared responsibility, contraceptives use excluding condoms were mainly intrauterine devices (IUD) 76%, tubal ligation 16% and vasectomy 8%. Although 24% of subjects had a history of contraceptive failure, effective contraceptive usage after failure among men remained lower 6% (TL and vasectomy) and moreover, 74% of naïve contraceptive users strongly rejected vasectomy. Demographic factors, awareness on FPs/SRH, discussing and making joint decision, and gender discrepancies were strongly associated with FPs/SRH unmet needs (P<0.001). CONCLUSION: FPs use roughly varied with the population's unmet needs and expectations that should be considered to sustain the strategies.

4.
Int J Biochem Cell Biol ; : 105974, 2021 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-33831591

RESUMO

Homocysteine (Hcy) is a strong and independent risk factor of atherosclerosis. It can accelerate atherosclerosis through increased production of inflammatory factors, especially interleukin-1 ß (IL-1ß), while the precise mechanisms remain to be well elucidated. In this study, we investigated the role of the tumor suppressor gene SNF5 related to Switch/Sucrose Non-Fermentable complex (SWI/SNF) in the occurrence and development of atherosclerosis induced by Hcy. Using Hyperhomocysteinemia (HHcy) atherosclerotic model with apolipoprotein E knockout (ApoE-/-) mice fed with high-methionine diet, we showed that Hcy aggravates inflammation in macrophages during the atherosclerotic plaque formation. Further analysis showed that SNF5 promotes IL-1ß expression and secretion. In addition, due to the existence of H3K4 methylation signals in the vicinity of IL-1ß, we found that Hcy significantly promotes the expression of H3K4me1, and lysine-specific histone demethylase 1A (KDM1A) acts as a transcriptional repressor to regulate the expression of H3K4me1 by demethylating H3K4me1. In summary, our results demonstrated that Hcy up-regulates the expression of SNF5 through KDM1A, resulting in an increased level of H3K4me1 modification and IL-1ß in macrophages, which in turn promotes the formation of atherosclerosis. Our study will provide more evidence for further revealing the specific mechanism of Hcy-induced inflammation and the diagnosis, prevention, and treatment of atherosclerosis.

5.
Respir Res ; 22(1): 89, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33752670

RESUMO

OBJECTIVE: Thise study is aimed to identify the biomarkers for predicting refractory Mycoplasma pneumoniae pneumonia in Chinese children at the time of the hospital admission. METHODS: The case control study retrospectively analyzed the clinical characteristics and laboratory results of Chinese pediatric patients presenting with common and refractory Mycoplasma pneumoniae pneumonia (CMPP and RMPP). Overall, there were 216 cases in the CMPP group and 88 cases in the RMPP group. Venous blood was collected, and serum ferritin (SF), lactate dehydrogenase (LDH), D-dimer, C-reactive protein (CRP), procalcitonin (PCT), neutrophil count/lymphocyte count (NLR), and other indexes were measured. A single factor analysis, an ROC curve analysis, and a logistic regression analysis were used to determine the independent risk factors of RMPP and find combination of initial markers for RMPP. RESULTS: There were significant differences between the RMPP group and the CMPP group in mean SF (529.82 [357.86] vs. 147.22 [122.68] ng/mL), LDH (522.08 [389.08] vs. 286.85 [101.02] U/L), D-dimer (6.65 [5.66] vs. 1.46 [2.45] µg/mL), CRP (62.80 [52.15] vs. 19.03 [24.50] mg/L), PCT (0.80 [2.61] vs. 0.16 [0.44]) ng/mL, and NLR (4.14 [2.52] vs. 2.62 [1.55]), with P < 0.05 for each comparison. ROC cut-off values of the above indexes were 329.01 ng/mL, 375.50 U/L, 2.10 µg/mL, 43.08 mg/L, 0.08 ng/mL, and 2.96, respectively. The logistic regression analysis showed that SF, D-dimer, and CRP are independent risk factors to predict RMPP. CONCLUSION: SF, D-dimer, and CRP are statistically significant biomarkers to predict RMPP in Chinese children patients in the settings of pediatric emergency department.

6.
Environ Pollut ; 277: 116873, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33714789

RESUMO

Triclocarban (TCC), an antibacterial agent widely used in personal care products, can affect embryonic development. However, the specific molecular mechanism of TCC-induced embryonic developmental damage remains unclear. In this study, TCC exposure was found to increase the expression of tmbim4 gene in zebrafish embryos. The tmbim4 mutant embryos are more susceptible to TCC exposure than wild-type (WT) embryos, with tmbim4 overexpression reducing TCC-induced embryonic death in the former. Exposure of tmbim4 mutant larvae to 400 µg/L TCC substantially increased apoptosis in the hindbrain and eyes. RNA-sequencing of WT and tmbim4 mutant larvae indicated that knockout of the tmbim4 gene in zebrafish affects the autophagy pathway. Abnormalities in autophagy can increase apoptosis and TCC exposure caused abnormal accumulation of autophagosomes in the hindbrain of tmbim4 mutant zebrafish embryos. Pretreatment of TCC-exposed tmbim4 mutant zebrafish embryos with autophagosome formation inhibitors, substantially reduced the mortality of embryos and apoptosis levels. These results indicate that defects in the tmbim4 gene can reduce zebrafish embryo resistance to TCC. Additionally, apoptosis induced by abnormal accumulation of autophagosomes is involved in this process.


Assuntos
Poluentes Químicos da Água , Peixe-Zebra , Animais , Apoptose , Autofagia , Carbanilidas , Embrião não Mamífero , Desenvolvimento Embrionário , Larva , Poluentes Químicos da Água/toxicidade
7.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 37(3): 240-245, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33766232

RESUMO

Objective To study the role of long non-coding RNA growth arrest specific transcript 5 (lncGAS5) in the autophagy of hepatocytes induced by homocysteine (Hcy). Methods HL7702 human hepatocyte cells were cultured in vitro and divided into control group and Hcy group. Western blotting was used to detect the expression levels of microtubule-associated protein 1 light chain 3B (LC3B) and P62. The cells were transfected with mRFP-GFP-LC3 adenovirus to observe the autophagy flow with laser scanning confocal microscope. Real-time quantitative PCR was performed to detect the expression level of lncGAS5. lncGAS5 small interfering RNA (si-lncGAS5) and negative control small interfering RNA (si-NC) were transfected into the cells. After the transfected cells were treated with Hcy, the changes of LC3B, P62 and autophagy flow were analyzed with the above methods. Results Compared with the control group, the LC3BII/LC3BI ratio increased and the expression of P62 protein decreased in the Hcy group. When the lever of Hcy lifted, the number of autophagosomes and autolysosomes and the expression of lncGAS5 increased in the cells. After knock-down of lncGAS5, the ratio of LC3BII/LC3BI decreased and the expression of P62 increased. Moreover, the number of autophagosomes and autolysosomes were reduced in the cells. Conclusion lncGAS5 can promote the autophagy of hepatocytes induced by Hcy.


Assuntos
RNA Longo não Codificante , Autofagia/genética , Hepatócitos , Homocisteína/farmacologia , Humanos , RNA Longo não Codificante/genética , RNA Nucleolar Pequeno
8.
Brain Behav ; 11(4): e02046, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33599398

RESUMO

INTRODUCTION: To explore the changes in C-reactive protein (CRP) level in different regions of one old offspring rats exposed to prenatal stress (PS). METHODS: The rat model was constructed with prenatal restraint stress on pregnant dams on days 14-20 of gestation. Offspring rats were randomly divided into PS susceptibility (PS-S) group and control (CON) group. Behavioral experiments including sucrose preference test (SPT), open-field test (OFT), and forced swimming test (FST) were used to measure depressive-like behaviors. Immunohistochemistry, qRT-PCR, and Western blotting were applied to detect the changes in CRP level. RESULTS: The results showed that PS could cause depressive-like behaviors in all SPT, OFT, and FST. Concomitantly, CRP mRNA and protein expression significantly increased in hippocampus, prefrontal cortex, and hypothalamus in the PS-S group when compared that in the CON group, while no significantly changes in liver, heart, olfactory bulb, striatum, and cerebellum in the PS-S group when compared that in the CON group. CONCLUSION: Increasing of CRP expression in hippocampus, prefrontal cortex, and hypothalamus may play a critical role in the mechanism under depressive-like behavior in offspring rats exposed to PS.

9.
Mol Oncol ; 15(4): 1054-1068, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33540470

RESUMO

Epidermal growth factor receptor (EGFR) is often overexpressed in head and neck squamous cell carcinoma (HNSCC) and represents a top candidate for targeted HNSCC therapy. However, the clinical effectiveness of current Food and Drug Administration (FDA)-approved drugs targeting EGFR is moderate, and the overall survival rate for HNSCC patients remains low. Therefore, more effective treatments are urgently needed. In this study, we generated a novel diphtheria toxin-based bivalent human epidermal growth factor fusion toxin (bi-EGF-IT) to treat EGFR-expressing HNSCC. Bi-EGF-IT was tested for in vitro binding affinity, cytotoxicity, and specificity using 14 human EGFR-expressing HNSCC cell lines and three human EGFR-negative cancer cell lines. Bi-EGF-IT had increased binding affinity for EGFR-expressing HNSCC compared with the monovalent version (mono-EGF-IT), and both versions specifically depleted EGFR-positive HNSCC, but not EGFR-negative cell lines, in vitro. Bi-EGF-IT exhibited a comparable potency to that of the FDA-approved EGFR inhibitor, erlotinib, for inhibiting HNSCC tumor growth in vivo using both subcutaneous and orthotopic HNSCC xenograft mouse models. When tested in an experimental metastasis model, survival was significantly longer in the bi-EGF-IT treatment group than the erlotinib treatment group, with a significantly reduced number of metastases compared with mono-EGF-IT. In addition, in vivo off-target toxicities were significantly reduced in the bi-EGF-IT treatment group compared with the mono-EGF-IT group. These results demonstrate that bi-EGF-IT is more effective and markedly less toxic at inhibiting primary HNSCC tumor growth and metastasis than mono-EGF-IT and erlotinib. Thus, the novel bi-EGF-IT is a promising drug candidate for further development.

10.
Aging (Albany NY) ; 13(4): 5226-5237, 2021 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-33535188

RESUMO

Few studies have investigated whether associations between smoking, sex hormone levels, and symptoms of late-onset hypogonadism (LOH) in men are affected by age. This multi-center, cross-sectional study involving 6,296 men aged 40-79 years was conducted between June 1, 2013 and August 31, 2016 in 6 provinces of China. Total testosterone, free testosterone, and Aging Males' Symptoms scale (AMS) scores were compared depending on smoking status and the number of cigarettes smoked. Total testosterone was higher in smokers than in non-smokers in all except the 70-79 year old subgroup. Free testosterone was higher in smokers than non-smokers for the 40-49 and 50-59 year old subgroups, but not the 60-69 and 70-79 year old subgroups. Total testosterone was positively associated with number of cigarettes consumed in smokers aged 40-49 and 50-59 years. Sexual and somatic AMS scores were higher in current and ex-smokers than in non-smokers in all age subgroups from 40 to 79 years and were negatively associated with cigarette consumption in smokers aged 40-49 years. These results indicate that, as men age, the positive association between smoking and testosterone weakens, while the positive association between smoking and LOH symptoms becomes stronger.

11.
J Sex Res ; : 1-9, 2021 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-33528275

RESUMO

Western studies have demonstrated that female sexual assertiveness is positively associated with sexual satisfaction in a committed heterosexual relationship. Furthermore, the ability of women to refuse sex has been shown to protect them from unwanted sexual contact and to lead to fewer sexual victimization experiences and less risky sexual behavior. However, there is little research on female sexual assertiveness that included both the own (actor effect) and partner (partner effect) impact of female sexual assertiveness on sexual satisfaction from a dyadic approach, involving both members of a couple. The data for the present study came from a representative sample of Chinese couples collected by the Hong Kong Family Planning Association in 2017. Using the actor-partner interdependence model, the results indicated that female sexual initiation was positively associated with both their own and their partner's sexual satisfaction. However, female sexual refusal was not associated with the sexual satisfaction of either party. These findings suggest the importance of adopting a dyadic approach in sex research to capture the mutual influences between partners. Couples should be encouraged to express their sexual desires and refuse unwanted sex requests honestly to improve their and their partner's sexual satisfaction.

12.
Toxins (Basel) ; 13(2)2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33499131

RESUMO

Two different types of polycyclic ether toxins, namely brevisulcenals (KBTs) and brevisulcatic acids (BSXs), produced by the red tide dinoflagellate Karenia brevisulcata, were the cause of a toxic incident that occurred in New Zealand in 1998. Four major components, KBT-F, -G, -H, and -I, shown to be cytotoxic and lethal in mice, were isolated from cultured K. brevisulcata cells, and their structures were elucidated by spectroscopic analyses. New analogues, brevisulcenal-A1 (KBT-A1) and brevisulcenal-A2 (KBT-A2), toxins of higher polarity than that of known KBTs, were isolated from neutral lipophilic extracts of bulk dinoflagellate culture extracts. The structures of KBT-A1 and KBT-A2 were elucidated as sulfated analogues of KBT-F and KBT-G, respectively, by NMR and matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI TOF/TOF), and by comparison with the spectra of KBT-F and KBT-G. The cytotoxicities of the sulfate analogues were lower than those of KBT-F and KBT-G.

13.
Reproduction ; 161(2): 99-109, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33434164

RESUMO

Indian hedgehog (Ihh) signaling regulates endometrial receptivity and is an indispensable mediator of embryonic implantation. Hedgehog signaling is known to regulate autophagy, and aberrant regulation of autophagy is critically implicated in the pathogenesis of endometriosis and adenomyosis. However, potential dysregulation of Ihh signaling and its role in autophagy modulation in these diseases remain obscure. In this study, we found that components of Ihh signaling were significantly decreased, whereas the autophagy marker protein, LC3BII, was significantly increased in endometrial tissues of women with endometriosis or adenomyosis. Inhibition of Ihh signaling with the small-molecule inhibitor GANT61 or Gli1 silencing in primary endometrial stromal cells increased autophagic activity, as measured by LC3 turnover assay and tandem mCherry-eGFP-LC3B fluorescence microscopy. Furthermore, we observed that GANT61 treatment significantly attenuated hydrogen peroxide-induced cell death, whereas disruption of autophagy with chloroquine diminished this effect. Collectively, these findings reveal that Ihh signaling is suppressed in endometrial tissues of patients with endometriosis or adenomyosis. This abnormal decrease may contribute to endometrial autophagy activation, which may promote aberrant survival of endometrial cells in ectopic sites in these two gynecological diseases.

14.
J Sex Marital Ther ; : 1-23, 2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33504270

RESUMO

Substantial evidence has demonstrated that sexual dysfunction is negatively associated with the sexual satisfaction of individuals in a committed heterosexual relationship. However, little is known about their relationship based on couple data, especially in non-Western societies. We extended this study to examine the extent to which men's and women's sexual dysfunction were associated with their own as well as their partners' sexual satisfaction. Participants were 1,014 heterosexual couples who participated in a community-based survey in Hong Kong in 2017. Using the actor-partner interdependence model and structural equation modeling, our results indicated that the dyadic model fit the data better than the individual model. Women's orgasm and men's sexual desire functioning were significant predictors of both own and partner's sexual satisfaction, and these two domains had similar effects on the sexual satisfaction of both men and women. These findings are discussed in terms of the importance of taking a dyadic approach to research and enhance sexual health and well-being of heterosexual couples.

15.
Bioorg Med Chem Lett ; 35: 127783, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33422607

RESUMO

A new unique sesquiterpene lactone, bicyclolamellolactone A (1), was isolated together with two known monocyclofarnesol-type sesquiterpenes, lamellolactones A (2) and B (3), from the Indonesian marine sponge Lamellodysidea sp. (cf. L. herbacea). The planar structure of 1 was assigned based on its spectroscopic data (1D and 2D NMR, HRESIMS, UV, and IR spectra). The relative and absolute configuration of 1 was determined by comparison of its calculated and experimental electronic circular dichroism spectra in combination with NOESY correlations. Compounds 1-3 inhibited bone morphogenic protein (BMP)-induced alkaline phosphatase activity in mutant BMP receptor-carrying C2C12 cells with IC50 values of 51, 4.6, and 20 µM, respectively.

16.
World J Gastroenterol ; 26(46): 7416-7424, 2020 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-33362393

RESUMO

BACKGROUND: It is important to differentiate benign and malignant focal liver lesions (FLLs) accurately. Despite the wide use and acceptance of shear wave elastography (SWE), its value for assessing the elasticity of FLLs and differentiating benign and malignant FLLs is still investigational. Previous studies of SWE for FLLs used mean elasticity as the parameter to reflect the stiffness of FLLs. Considering the inhomogeneity of tumor stiffness, maximal elasticity (Emax) might be the suitable parameter to reflect the stiffness of FLLs and to differentiate malignant FLLs from benign ones. AIM: To explore the value of SWE with Emax in differential diagnosis of solid FLLs. METHODS: We included 104 solid FLLs in 95 patients and 50 healthy volunteers. All the subjects were examined using conventional ultrasound (US) and virtual touch tissue quantification(VTQ) imaging. A diagnosis of benign or malignant FLL was made using conventional US. Ten VTQ values were acquired after 10 consecutive measurements for each FLL and each normal liver, and the largest value was recorded as Emax. RESULTS: There were 56 cases of malignant FLLs and 48 cases of benign FLLs in this study. Emax of malignant FLLs (3.29 ± 0.88 m/s) was significantly higher than that of benign FLLs (1.30 ± 0.46 m/s, P < 0.01) and that of livers in healthy volunteers (1.15 ± 0.17 m/s, P < 0.01). The cut-off point of Emax was 1.945, and the area under the curve was 0.978. The sensitivity and specificity of Emax were 92.9% and 91.7%, respectively, higher (but not significantly) than those of conventional US (80.4% for sensitivity and 81.3% for specificity). Combined diagnosis of conventional US and Emax using parallel testing improved the sensitivity to 100% with specificity of 75%. CONCLUSION: SWE is a convenient and easy method to obtain accurate stiffness information of solid FLLs. Emax is useful for differential diagnosis of FLLs, especially in combination with conventional US.

17.
J Hazard Mater ; : 124371, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33248822

RESUMO

Novel nitrogen-doped carbon nanotubes encapsulating Fe3C nanocrystals coated paper-like sintered stainless steel fibers (PSSF) structured catalyst (Fe3C@NCNT/PSSF) was designed for continuous catalytic wet peroxide oxidation of phenol. Firstly, Fe3C@NCNT/PSSF was fabricated by directly growing the Fe3C encapsulated NCNTs onto the three-dimensional PSSF substrate through CVD method using melamine as precursor, the monolithic PSSF substrate served as a self-catalyzing agent for catalyst preparation. Secondly, the surface morphology and structure of Fe3C@NCNT/PSSF were investigated to optimize the synthesis condition. Then Fe3C@NCNT/PSSF was employed as a structured catalyst for continuous CWPO of phenol, effect of operating conditions was studied. Catalytic results showed that the encapsulated Fe3C nanoparticles significantly enhanced the degradation efficiency of phenol, and catalytic performance was improved with the increase of temperature. However, catalytic performance appeared unusual when residence time was considered, due to the effect of strongly polar surface of NCNTs on the contact efficiency between pollutants and hydroxyl radicals. Reusability experiments showed that catalytic performance of catalyst was improved with the increase of reusability cycles although the iron leaching concentration decreased, attributing to enhanced reaction within internal channel of Fe3C@NCNT. The fourth reaction run achieved a stable phenol conversion of 90%, TOC conversion around 41% under optimized conditions.

18.
Aging (Albany NY) ; 122020 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-33229621

RESUMO

OBJECTIVE: This systematic review and meta-analysis was aimed at determining whether paternal age is a risk factor for offspring birth defects. RESULTS: A total of 38 and 11 studies were included in the systematic review and meta-analysis, respectively. Compared with reference, fathers aged 25 to 29, young fathers (< 20 years) could increase the risk of urogenital abnormalities (OR: 1.50, 95 % CI: 1.03-2.19) and chromosome disorders (OR: 1.38, 95 % CI: 1.12-1.52) in their offsprings; old fathers (≥ 40 years) could increase the risk of cardiovascular abnormalities (OR: 1.10, 95 % CI: 1.01-1.20), facial deformities (OR: 1.08, 95 % CI: 1.00-1.17), urogenital abnormalities (OR: 1.28, 95 % CI: 1.07-1.52), and chromosome disorders (OR: 1.30, 95 % CI: 1.12-1.52). CONCLUSIONS: Our study indicated that paternal age is associated with a moderate increase in the incidence of urogenital and cardiovascular abnormalities, facial deformities, and chromosome disorders. METHODS: PubMed, Web of Science, the Cochrane Library, and Embase were searched for relevant literatures from 1960 to February 2020. The systematic review follows PRISMA guidelines. Relevant meta-analyses were performed.

19.
J Psychiatr Res ; 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33243459

RESUMO

Telomeres protect chromosome ends from degradation. Telomere length (TL) can be altered by aging and environmental stress. Shortened TL has been observed in peripheral blood leukocytes of alcohol dependent subjects and ethanol-exposed somatic cells. To understand the impact of ethanol on telomeres in pluripotent stem cells, we investigated the influence of ethanol on TL and the expression of six Shelterin complex subunit or telomere-regulating genes (POT1, RAP1, TIN2, TPP1, TRF1, and TRF2) in human embryonic stem cells (hESCs), which were exposed to 0, 25, 50, or 100 mM of ethanol for 3, 7, or 14 days. Ethanol-induced TL and Shelterin complex subunit gene expression changes were examined by quantitative polymerase chain reactions. Two-way ANOVA tests indicated that TL variation and expression changes of four associated Shelterin complex subunit genes (POT1, TPP1, TIN2, and TRF2) were mainly dependent on the length of ethanol exposure, while TRF1 and RAP1expression was influenced by ethanol concentration, exposure time, and the interaction of ethanol concentration and exposure time. Tukey's multiple comparison tests showed that TL and the expression of POT1, RAP1, TIN2, TPP1, and TRF1 were decreased after a 7-day (versus a 3-day) ethanol exposure. However, the decreased expression of all six Shelterin complex subunit genes was recovered and TL was not further shortened after a 14-day (versus a 7-day) ethanol exposure, likely due to the adaptation of hESCs to ethanol-induced stress. Our study provided further evidence that TL is regulated and maintained by telomere-regulating genes in stem cells under ethanol stress.

20.
Environ Toxicol Pharmacol ; 81: 103523, 2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33191202

RESUMO

Wastewater and organic oxygen-demanding pollutants (ODPs) are produced by various factories in China, the United States and other countries. However, whether ODP affects reproductive health remains unclear. To investigate the impact of environmental concentrations of ODP exposure on reproductive health, adult male zebrafish were used to evaluated the effects ODP exposure on the fertility in this study. We found that exposure to ODP reduced the sperm motility of adult male zebrafish. Similarly, the testosterone content of the experimental zebrafish was obviously decreased. Transcription of immune response-related genes, including tumor necrosis factor (tnf)-α, il-1ß, and il-8, was upregulated upon exposure to ODP. Mating experiments indicated that the hatching time of the offspring embryos was clearly prolonged upon ODP exposure, but the embryo fertilization rate was not different. These results assumed that exposure to ODP at ambient concentrations visibly affected the sperm motility in adult zebrafish maybe due to the expression of immune response-related genes in the zebrafish male gonads and the release of pro-inflammatory mediators. Therefore, we assumed that the impact of ODP on the reproductive health of aquatic organisms cannot be ignored.

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