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1.
Carbohydr Polym ; 251: 117028, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33142587

RESUMO

In this work, the application of acid site-regulated solid acids in Se-functionalization of polysaccharide is evaluated for the first time, which aimed to further improve reaction efficiency and realize environmentally friendly chemistry. A series prepared MxOy/HZSM-5 catalysts possesses standard crystal structure, large specific surface area, pore volume, aperture as well as strong acidity. An efficient substitution of seleno-group on polysaccharide backbone is promoted by regulating the acid site of solid acids (Se content up to 15,170.49 µg/g) compared with the conventional Se-functionalization method (1703 µg/g). Strong Lewis and Brønsted acid sites lead to the driving forces toward low molecular mass polysaccharide fragments, but the deletion of main monosaccharide components is not observed. In summary, it is proved that solid acid can be employed in acid-dependent polysaccharide Se-functionalization which will promote useful in expanding our understanding of how to further develop polysaccharide resources.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33144505

RESUMO

Functional electrolyte is the key to stabilize the highly reductive lithium (Li) metal anode and the high-voltage cathode for long-life, high-energy-density rechargeable Li metal batteries (LMBs). However, fundamental mechanisms on the interactions between reactive electrodes and electrolytes are still not well understood. Recently localized high-concentration electrolytes (LHCEs) are emerging as a promising electrolyte design strategy for LMBs. Here, we use LHCEs as an ideal platform to investigate the fundamental correlation between the reactive characteristics of the inner solvation sheath on electrode surfaces due to their unique solvation structures. The effects of a series of LHCEs with model electrolyte solvents (carbonate, sulfone, phosphate, and ether) on the stability of high-voltage LMBs are systematically studied. The stabilities of electrodes in different LHCEs indicate the intrinsic synergistic effects between the salt and the solvent when they coexist on electrode surfaces. Experimental and theoretical analyses reveal an intriguing general rule that the strong interactions between the salt and the solvent in the inner solvation sheath promote their intermolecular proton/charge transfer reactions, which dictates the properties of the electrode/electrolyte interphases and thus the battery performances.

3.
Adv Mater ; : e2004898, 2020 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-33150628

RESUMO

High-energy-density batteries with a LiCoO2 (LCO) cathode are of significant importance to the energy-storage market, especially for portable electronics. However, their development is greatly limited by the inferior performance under high voltages and challenging temperatures. Here, highly stable lithium (Li) metal batteries with LCO cathode, through the design of in situ formed, stable electrode/electrolyte interphases on both the Li anode and the LCO cathode, with an advanced electrolyte, are reported. The LCO cathode can deliver a high specific capacity of ≈190 mAh g-1 and show greatly improved cell performances under a high charge voltage of 4.5 V (even up to 4.55 V) and a wide temperature range from -30 to 55 °C. This work points out a promising approach for developing Li||LCO batteries for practical applications. This approach can also be used to improve the high-voltage performance of other batteries in a broad temperature range.

4.
Protein Expr Purif ; : 105788, 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33221504

RESUMO

MEF2D-fusions have recently been identified as one of the major oncogenic drivers in precursor B-cell acute lymphoblastic leukemia (B-ALL). More importantly, they are often associated with patients with poor prognosis in B-ALL. To have a better understanding of the pathogenic mechanism underpinning MEF2D-fusions-driven leukemogenesis, it's essential to uncover the related structure information. In this study, we expressed and purified the MEF2D N-terminal DNA binding domain. The recombinant protein was engineered by cloning the encoding gene into the expression vector pET-32m. A series of chromatographic steps involving affinity, ion-exchange and gel-filtration chromatography were used to achieve a final purity of >95%. For the crystallization of the MEF2D-DNA complex, a double-stranded DNA encoding 5'-AACTATTTATAAGA-3' and 5'-TTCTTATAAATAGT-3' was used [1]. The MEF2D-DNA crystal with the size of about 20µm × 20µm × 20µm was obtained at a final concentration of 12mg ml-1 at the reservoir condition containing 30% PEG1500. The X-ray examination showed that the MEF2D-DNA crystal diffracted to 4.5 Å resolution, and belonged to space group P1, with unit-cell parameters of a = 77.2 Å, b = 77.2 Å, c = 231.4 Å.

5.
Technol Cancer Res Treat ; 19: 1533033820973282, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33176591

RESUMO

PURPOSE: Esophageal cancer is one of the most common malignancies worldwide. Ubiquitin-dependent degradation of regulatory proteins reportedly plays a central role in diverse cellular processes. This study investigated the expression levels of ubiquitin in esophageal squamous cell carcinoma tissues and the functions of ubiquitin in the context of esophageal squamous cell carcinoma progression. METHODS: The expression of ubiquitin in esophageal squamous cell carcinoma and normal esophageal samples was determined via immunohistochemistry. Serum ubiquitin levels were determined by enzyme-linked immunosorbent assay. The association between serum ubiquitin level and clinicopathological factors was analyzed. Real-time PCR analysis was employed to measure the mRNA levels of the ubiquitin coding genes ubiquitin B and ubiquitin C. Proliferation assays, colony formation assays, and Transwell-based assays were used to determine the influence of ubiquitin on cell growth and cell invasion. Proteomic analysis was performed to identify the proteins associated with ubiquitin. RESULTS: Ubiquitin expression in esophageal squamous cell carcinoma tissues was markedly higher than that in normal and tumor adjacent tissues. The levels of ubiquitin in esophageal squamous cell carcinoma serum samples were significantly higher than those in healthy controls. Serum ubiquitin levels were correlated with tumor stage and lymph node metastasis. To silence the expression of ubiquitin, we knocked down the ubiquitin coding genes ubiquitin B and ubiquitin C in TE-1 and Eca-109 cells. Silencing ubiquitin resulted in the suppression of cell growth, chemoresistance, colony formation and cell migration in esophageal squamous cell carcinoma cells. Proteomic analysis in esophageal squamous cell carcinoma cells showed that knockdown of ubiquitin coding genes deregulated the expression of 159 proteins (92 were upregulated and 67 were downregulated) involved in multiple pathways. These proteins included ferritin light chain, ferritin heavy chain, cellular retinoic acid-binding protein 2, and DNA replication factor 1. CONCLUSION: Ubiquitin expression is upregulated in esophageal squamous cell carcinoma tissues and serum samples. Serum ubiquitin levels were correlated with tumor stage and lymph node metastasis. Downregulation of ubiquitin suppresses the aggressive phenotypes of esophageal squamous cell carcinoma cells by complex mechanisms; ubiquitin may represent a novel target for the treatment of esophageal squamous cell carcinoma.

6.
Emerg Microbes Infect ; : 1-28, 2020 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-33179570

RESUMO

Shiga toxin (Stx)-producing Escherichia coli (STEC) can cause a wide range of symptoms from asymptomatic carriage, mild diarrhea to bloody diarrhea (BD) and hemolytic uremic syndrome (HUS). Besides the Stx, intimin, encoded by the eae gene, also plays a critical role in STEC pathogenesis. Herein, we investigated the prevalence and genetic diversity of eae among clinical STEC strains isolated from patients with diarrhea, BD, HUS as well as from asymptomatic STEC-positive individuals in Sweden with whole-genome sequencing. We found that 173 out of 239 (72.4%) of clinical STEC strains were eae positive. Six eae subtypes (ϵ1, γ1, ß3, θ, ζ and ρ) were identified, which were classified into 29 different genotypes. eae and its subtype γ1 were significantly overrepresented in O157:H7 strains isolated from BD and HUS patients. ϵ1 was associated with O121:H19 and O103:H2 strains, and ß3 to O26:H11 strains. The presence of stx 2+eae and stx 1+stx 2+eae was significantly more prevalent in strains from BD and HUS patients, respectively. The combination of a certain eae subtype (γ1) and stx subtype (stx 2 or stx 1+stx 2) is more likely to cause severe disease, suggesting the possibility of using eae genotypes in risk assessment of STEC infection. No association was observed between the presence of eae or subtypes or duration of stx shedding. In summary, this study demonstrated a high prevalence of eae in clinical STEC strains and considerable genetic diversity of eae in STEC strains in Sweden from 1994 through 2018, and revealed association between eae subtypes and disease severity.

7.
Mil Med Res ; 7(1): 53, 2020 11 05.
Artigo em Inglês | MEDLINE | ID: mdl-33148321

RESUMO

BACKGROUND: Acute mountain sickness (AMS) is the mildest form of acute altitude illnesses, and consists of non-specific symptoms when unacclimatized persons ascend to elevation of ≥2500 m. Risk factors of AMS include: the altitude, individual susceptibility, ascending rate and degree of pre-acclimatization. In the current study, we examined whether physiological response at low altitude could predict the development of AMS. METHODS: A total of 111 healthy adult healthy volunteers participated in this trial; and 99 (67 men and 32 women) completed the entire study protocol. Subjects were asked to complete a 9-min exercise program using a mechanically braked bicycle ergometer at low altitude (500 m). Heart rate, blood pressure (BP) and pulse oxygen saturation (SpO2) were recorded prior to and during the last minute of exercise. The ascent from 500 m to 4100 m was completed in 2 days. AMS was defined as ≥3 points in a 4-item Lake Louise Score, with at least one point from headache wat 6-8 h after the ascent. RESULTS: Among the 99 assessable subjects, 47 (23 men and 24 women) developed AMS at 4100 m. In comparison to the subjects without AMS, those who developed AMS had lower proportion of men (48.9% vs. 84.6%, P < 0.001), height (168.4 ± 5.9 vs. 171.3 ± 6.1 cm, P = 0.019), weight (62.0 ± 10.0 vs. 66.7 ± 8.6 kg, P = 0.014) and proportion of smokers (23.4% vs. 51.9%, P = 0.004). Multivariate regression analysis revealed the following independent risks for AMS: female sex (odds ratio (OR) =6.32, P < 0.001), SpO2 change upon exercise at low altitude (OR = 0.63, P = 0.002) and systolic BP change after the ascent (OR = 0.96, P = 0.029). Women had larger reduction in SpO2 after the ascent, higher AMS percentage and absolute AMS score. Larger reduction of SpO2 after exercise was associated with both AMS incidence (P = 0.001) and AMS score (P < 0.001) in men but not in women. CONCLUSIONS: Larger SpO2 reduction after exercise at low altitude was an independent risk for AMS upon ascent. Such an association was more robust in men than in women. TRIAL REGISTRATION: Chinese Clinical Trial Registration, ChiCTR1900025728 . Registered 6 September 2019.

8.
Chem Rev ; 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33174427

RESUMO

High-energy rechargeable lithium (Li) metal batteries (LMBs) with Li metal anode (LMA) were first developed in the 1970s, but their practical applications have been hindered by the safety and low-efficiency concerns related to LMA. Recently, a worldwide effort on LMA-based rechargeable LMBs has been revived to replace graphite-based, Li-ion batteries because of the much higher energy density that can be achieved with LMBs. This review focuses on the recent progress on the stabilization of LMA with nonaqueous electrolytes and reveals the fundamental mechanisms behind this improved stability. Various strategies that can enhance the stability of LMA in practical conditions and perspectives on the future development of LMA are also discussed. These strategies include the use of novel electrolytes such as superconcentrated electrolytes, localized high-concentration electrolytes, and highly fluorinated electrolytes, surface coatings that can form a solid electrolyte interphase with a high interfacial energy and self-healing capabilities, development of "anode-free" Li batteries to minimize the interaction between LMA and electrolyte, approaches to enable operation of LMA in practical conditions, etc. Combination of these strategies ultimately will lead us closer to the large-scale application of LMBs which often is called the "Holy Grail" of energy storage systems.

9.
J Steroid Biochem Mol Biol ; 205: 105775, 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33130021

RESUMO

Bile acids (BAs) are currently considered as causative agents for Cholangiocarcinoma (CCA). However, the profile of circulating BAs in CCA have not been well characterized. The aim of this study was to describe the alterations of BAs metabolism in patients with CCA compared to benign biliary diseases (BBD) and healthy controls (HC), and to discover the specific BAs as biomarkers for CCA diagnosis. The concentrations of 15 BAs in plasma were measured in a total of 329 subjects, including patients with BBD, CCA, gallbladder cancer (GC), hepatocellular carcinoma (HCC), and healthy subjects, using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Binary logistic regression analysis was used to build a diagnostic model for CCA. An imbalance in the ratio of conjugated to unconjugated BAs was observed in CCA patients compared to BBD and HC groups, with higher conjugated BAs and lower unconjugated BAs. A panel of 2 BA metabolites consisting of CDCA and TCDCA showed high diagnostic performance for CCA versus BBD and CCA versus HC, with higher AUC, sensitivity and specificity than carbohydrate antigen 19-9 (CA 199), clinically employed CCA biomarker. Importantly, HCC and GC samples were also included to confirm specificity of the BA biomarkers for CCA diagnosis. In summary, specific changes in plasma concentrations of BAs may serve as diagnostic biomarkers for distinguishing CCA from BBD and HC, with higher performance than CA199.

10.
Clin Nephrol ; 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33211000

RESUMO

BACKGROUND: The aim of this study was to investigate the predictors of renal outcomes in crescentic and mixed class of ANCA-associated glomerulonephritis. MATERIALS AND METHODS: We systematically reviewed the medical records of patients with ANCA-associated glomerulonephritis admitted to our hospital from December 2008 to December 2018, and found 30 patients with crescentic and 40 patients with mixed ANCA-associated glomerulonephritis. RESULTS: End-stage renal disease developed in 33.3 and 25% patients over a median follow-up of 45.1 and 46.7 months in the crescentic and mixed group, respectively. There was no significant difference in renal survival rates between the two histological subgroups (log-rank p = 0.558). In the Cox regression model, old age, lower estimated glomerular filtration rate (eGFR), lower normal glomeruli ratio, and a higher tubular atrophy and interstitial fibrosis ratio were significantly associated end-stage renal disease (p < 0.05 for all). Among our patients, 17.1% were at low risk, 57.1% were at medium risk, and 25.7% were at high risk according to antineutrophil cytoplasmic antibody renal risk score and end-stage renal disease developed in 8.3, 40, and 66.7%, respectively (p = 0.024). CONCLUSION: These findings indicated that the renal risk score was a better prognostic tool than Berden's classification in a cohort with crescentic and mixed histologic categories.

11.
Emerg Microbes Infect ; : 1-41, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33131455

RESUMO

In the era of "treat all", using normalization of CD4 counts as the main evaluation parameter of complete immune restoration for HIV-1 patients under antiretroviral therapy (ART) might not enough. A comprehensive evaluation system more accurately reflecting immune restoration are urgently needed. We established a retrospective cohort of 91,805 HIV-1 patients from 17 tertiary hospitals based on China's National Free Antiretroviral Treatment Program database from 2005 to 2018. Immune restoration and mortality were assessed. ART was started in HIV-1 patients with baseline CD4 counts <50, 50-199, 200-349, 350-499, and ≥500 cells/µL, and results showed an increase in the median CD4 counts to 445 (12-year), 467 (12-year), 581 (11-year), 644 (7-year), and 768 cells/µL (5-year), as well as the CD4/CD8 ratio to 0.59 (12-year), 0.65 (12-year), 0.79 (11-year), 0.82 (7-year), 0.9 (5-year), respectively. Meanwhile, the median CD8 count was relatively high (median range 732-845 cells/µL), regardless of the baseline CD4 counts, even after the longest follow-up period. Furthermore, the probabilities of death in patients achieving a combination of CD4 counts ≥500 cells/µL and CD4/CD8 ratio ≥0.8 were significantly lower than those in patients achieving either CD4 counts ≥500 cells/µL (2.77% vs 3.50%, p = 0.02) or CD4/CD8 ≥0.8 (2.77% vs 4.28%, p < 0.001) after 12 years of ART. In this study, a new binary-indicator would accurately assess the immune system and predict the probability of death after long-term ART in the era of "treat all".

12.
Plants (Basel) ; 9(10)2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33065969

RESUMO

Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the present study, we identified 100 NBS-LRR genes in the kiwifruit genome and they were grouped into six distinct classes based on their domain architecture. Of the 100 genes, 79 are truncated non-regular NBS-LRR genes. Except for 37 NBS-LRR genes with no location information, the remaining 63 genes are distributed unevenly across 18 kiwifruit chromosomes and 38.01% of them are present in clusters. Seventeen families of cis-acting elements were identified in the promoters of the NBS-LRR genes, including AP2, NAC, ERF and MYB. Pseudomonas syringae pv. actinidiae (pathogen of the kiwifruit bacterial canker) infection induced differential expressions of 16 detected NBS-LRR genes and three of them are involved in plant immunity responses. Our study provides insight of the NBS-LRR genes in kiwifruit and a resource for the identification of new R-genes in the fruit.

13.
Artigo em Inglês | MEDLINE | ID: mdl-33031947

RESUMO

OBJECTIVE: Rapid, reliable, and easy-to-implement diagnostics that can be adapted in early SARS-CoV-2 diagnosis are critical to combat the epidemic. SARS-CoV-2 nucleocapsid protein (NP) is an ideal target for viral antigen-based detection. A rapid and convenient method was developed based on fluorescence immunochromatographic (FIC) assay to detect the SARS-CoV-2 NP antigen. However, the accuracy of this diagnostic method needs to be examined. METHODS: This prospective study was carried out between February 10 and 15, 2020 in 7 hospitals of Wuhan and 1 hospital of Chongqing, China. Participants with clinically suspected SARS-CoV-2 infection were enrolled. NP antigen testing by FIC assay and nucleic acid (NA) testing by RT-PCR were performed simultaneously in a blind manner with the same nasopharyngeal swab sample. The diagnostic accuracy of NP antigen testing was calculated by taking NA testing of RT-PCR as reference standard, in which samples with cycle threshold (Ct) value ≤ 40 were interpreted as SARS-CoV-2 positives. RESULTS: A total of 253 participants were enrolled and 2 participants were excluded from the analyses due to invalid NP testing results. Of 251 participants (99.2%) that were included in the diagnostic accuracy analysis, a total of 201 participants (80.1%) had a Ct value ≤40. With Ct value 40 as the cut-off of NA testing, the sensitivity, specificity, and percent agreement of the FIC assay was 75.6% (95% CI 69.0%-81.3%), 100% (95% CI 91.1%-100%), and 80.5% (95% CI 75.1%-84.9%), respectively. CONCLUSIONS: With RT-PCR assay as reference standard, NP antigen testing by FIC assay shows high specificity and relative high sensitivity in SARS-CoV-2 diagnosis in the early phase of infection.

14.
Chin J Integr Med ; 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33037518

RESUMO

OBJECTIVE: To evaluate whether the efficacy of Getong Tongluo Capsule (, GTC, consisted of total flavone of Radix Puerariae) on improving patients' quality of life and lowering blood pressure are superior to the extract of Ginkgo biloba (EGB) for patients with convalescent-phase ischemic stroke and primary hypertension. METHODS: This randomized, positive-drug- and placebo-controlled, double-blind trial was conducted from September 2015 to October 2017. Totally 477 eligible patients from 18 hospitals in China were randomly assigned in a 2:1:1 ratio to the following interventions, twice a day for 12 weeks: (1) GTC 250 mg plus EGB-matching placebo 40 mg (237 cases, GTC group), (2) EGB 40 mg plus GTC-matching placebo 250 mg (120 cases, EGB group) or (3) GTC-matching placebo 250 mg plus EGB-matching placebo 40 mg (120 cases, placebo group). Moreover, all patients were orally administered aspirin enteric-coated tablets 100 mg, once a day for 12 weeks. The primary outcome was the Barthel Index (BI). The secondary outcomes included the control rate of blood pressure and National Institutes of Health Stroke Scale (NIHSS) scores. The incidence and severity of adverse events (AEs) were calculated and assessed. RESULTS: The BI relative independence rates, the clinical recovery rates of NIHSS, and the total effective rates of NIHSS in the GTC and EGB groups were significantly higher than the placebo group at 12 weeks after treatment (P<0.05), and no statistical significance was found between the GTC and EGB groups (P>0.05). The control rate of blood pressure in the GTC group was significantly higher than the EGB and placebo groups at 12, 18 and 24 weeks after treatment (P<0.01). There were no statistically significant differences in the incidences of AEs, adverse drug reactions, or serious AEs among the 3 groups (P>0.05). CONCLUSION: GTC exhibited significant efficacy in improving patients' quality of life as well as neurological function and controlling hypertension. (Registration No. ChiCTR1800016667).

15.
J Fluoresc ; 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33113563

RESUMO

In this study, a new Cd(II)-bearing coordination polymer with the chemical formula of {[Cd4(meda)3(dpe)4(H2O)4]·(NO3)2·2(H2O)}n (1, H2meda = 3,3'-methylenedibenzoic acid, dpe = 1,2-di(pyridin-4-yl)ethane) has been successfully prepared by reaction of Cd(NO3)·4H2O with a V-shape carboxyl ligand H2meda along with the linear dipyridine ligand dpe under the hydrothermal conditions. Due to its intensive luminescence, complex 1 could be utilized as the sensor of detecting Al3+ ion, and its detection limit is 4 × 10-6 M. Firstly, the toxicity of the compound on the normal liver cells was determined with Cell Counting Kit-8 detection kit. The triglyceride in liver cells was detected by detection kit after compound treatment and the relative expression of 15-lox and 12-lox in L02 cells was also measured by RT-PCR after compound treatment. In addition, multiple functional groups that provided by the synthesized Cd(II) complex have been studied by using molecular docking simulation for the confirmation of possible binding modes that formed between ligand and receptor.

16.
Neurobiol Dis ; 146: 105133, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33049318

RESUMO

Dendritic spines are specialized structures involved in neuronal processes on which excitatory synaptic contact occurs. The microtubule cytoskeleton is vital for maintaining spine morphology and mature synapses. Spastin is related to microtubule-severing proteases and is involved in synaptic bouton formation. However, it is not yet known if spastin can be modified by Small Ubiquitin-like Modifier (SUMO) or how this modification regulates dendritic spines. Spastin was shown to be SUMOylated at K427, and its deSUMOylation promoted microtubule stability. In addition, SUMOylation of spastin was shown to affect signalling pathways associated with long term synaptic depression. SUMOylated spastin promoted the development of dendrites and dendritic spines. Moreover, SUMOylated spastin regulated endocytosis and affected the transport of the AMPA receptor, GluA1. Our findings suggest that SUMOylation of spastin promotes GluA1 internalization and regulates dendritic spine morphology through targeting of microtubule dynamics.

17.
Biomed Environ Sci ; 33(9): 660-669, 2020 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-33106211

RESUMO

Objective: To estimate the association between three B-vitamin intakes and sociodemographic factors among adults in China. Methods: We derived our data from the China Health and Nutrition Survey (CHNS) among 12,241 individuals aged 18-64 years. Log binomial regression was used to estimate adjusted prevalence ratios for factors associated with the inadequate intake of B-vitamins. Results: Females with low incomes and living in the north had a higher prevalence of inadequate riboflavin intake than those with high incomes and living in the south. Both males and females living in a village had a higher prevalence of inadequate riboflavin intake than adults living in a city. Adults with low income, low education, and living in the north or in a village had a higher prevalence of inadequate niacin intake than adults with a high income, high education, and living in the south or in a city. Conclusion: We found that income, region, and area of residence were associated with riboflavin intake. Education, income, region, and area of residence were associated with niacin intake. Well-tailored strategies and policies are needed to improve nutritional status in China.


Assuntos
Niacina/análise , Riboflavina/análise , Tiamina/análise , Complexo Vitamínico B/análise , Adulto , China , Estudos de Coortes , Dieta , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Inquéritos Nutricionais , Fatores Socioeconômicos , Adulto Jovem
18.
J Phys Chem B ; 124(44): 9812-9819, 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33089685

RESUMO

The significance of karrikins (KARs) in plant physiology opens a door for their application in the agricultural production. As the first event of the whole signaling pathway, the binding of smoke-derived signal molecules KARs to the receptor protein KAI2 triggers the germination of the primary dormant seeds of all angiosperms, not just the "fire-prone" taxa. In the present study, all-atom molecular dynamics simulations, along with the accurate estimation of the ligand-receptor binding free energy, were used to investigate the atomic level interaction of all the members of the KARs family (from KAR1 to KAR6) with the receptor ShKAI2iB, an intermediate-evolving KAI2 from Striga hermonthica. The calculated binding energy value of KAR1 to ShKAI2iB, -5.64 kcal/mol, is in good agreement with the available experimental data, -5.67 kcal/mol. The further analysis of the detailed interaction between each KAR and the protein reveals the primary reasons for the difference of the affinity of the diverse ligands with the receptor and displays the regional characteristics of the protein's active site. Our research will not only provide clues for the study of equivalent endogenous phytohormone, but also contribute to the development of synthetic germinating chemicals.

19.
Curr Alzheimer Res ; 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33030131

RESUMO

BACKGROUND AND OBJECTIVE: Hypoxic preconditioning (HPC) has been well established to trigger endogenous mechanisms of neuroprotection basing on models of hypoxic and ischemic diseases in the central nervous system (CNS). However, its effects against Alzheimer's disease (AD) still lack substantial evidence and in-depth exploration. The present study aimed to investigate the impacts of HPC on AD-related memory decline and amyloid-ß (Aß) pathology in AßPP/PS1 transgenic mice. METHODS: Seven-week-old AßPP/PS1 transgenic mice were randomized into HPC and non-HPC groups. The HPC groups were treated with early and repetitive HPC for four weeks, while the non-HPC group was raised under normoxia condition. All the animals were then raised until the age of 28 weeks when Morris water maze tests were conducted to examine the animals' spatial memory. Indicators for Aß pathology (soluble Aß levels and numbers of Aß plaques) and the expression of relevant proteins were measured to explore potential mechanisms. RESULTS: The results showed that HPC ameliorated memory decline and Aß pathology in AßPP/PS1 mice. The protein levels of Amyloid-ß Precursor Protein (AßPP) and ß-site APP Cleaving Enzyme 1 (BACE1) were reduced while that of Hypoxic inducible factor 1α (HIF-1α) was elevated in HPC groups. CONCLUSION: HPC might be a promising strategy for AD intervention. Its potential protection might be realized via downregulating the expressions of AßPP and BACE1 and hence inhibiting Aß pathology. Notably, HIF-1α might play a key role in mediating subsequent neuroadaptive changes following HPC.

20.
Vet Parasitol ; 287: 109267, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33091629

RESUMO

Adonis coerulea Maxim. as a folk medicine, presented acaricidal acitvity. However, the mode of action and active compounds were unclear. In this study, using proteomics and surface plasmon resonance (SPR) technology the mode of action and active compounds of A. coerulea were investigated, as well as a sensitive and environmentally friendly analytical method developed. Proteomics analysis results showed that after treatment of mites with A. coerulea methanol extract (MEAC), 135 proteins were differentially expressed, most of them enriched in the myocardium pathway and participated in the function of the inflated cystic organ. Na+-K+-ATPase may be a potential target. Then, it was used as a target to capture the compounds from the extract using a SPR test. Twelve compounds were found, five compounds, namely ellagic acid, ouabain, convallatoxin, strophanthidin and cymarin presented the higher affinity with Na+-K+-ATPase in molecular docking test. Further study showed that the latter four compounds presented the stronger cytotoxicity and the inhibitory effect on Na+-K+-ATPase with IC50 values ranging with 2.38-0.56 µg/mL, and also showed toxicity against Psoroptes cuniculi. These results indicated that MEAC presented toxicity against mites by inhibiting Na+-K+-ATPase, and cardiac glycosides may be active compounds of this plant in terms of its acaricidal activity. Only 10 g of plant was used to investigate its active compounds. This study lays the foundation for developing sensitive methods for active compound detection.

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