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1.
Neural Regen Res ; 16(2): 333-337, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32859793

RESUMO

The arcuate fasciculus is a critical component of the neural substrate of human language function. Surgical resection of glioma adjacent to the arcuate fasciculus likely damages this region. In this study, we evaluated the outcome of surgical resection of glioma adjacent to the arcuate fasciculus under the guidance of magnetic resonance imaging and diffusion tensor imaging, and we aimed to identify the risk factors for postoperative linguistic deficit. In total, 54 patients with primary glioma adjacent to the arcuate fasciculus were included in this observational study. These patients comprised 38 men and 16 women (aged 43 ± 11 years). All patients underwent surgical resenction of glioma under the guidance of magnetic resonance imaging and diffusion tensor imaging. Intraoperative images were updated when necessary for further resection. The gross total resection rate of the 54 patients increased from 38.9% to 70.4% by intraoperative magnetic resonance imaging. Preoperative language function and glioma-to-arcuate fasciculus distance were associated with poor language outcome. Multivariable logistic regression analyses showed that glioma-to-arcuate fasciculus distance was the major independent risk factor for poor outcome. The cutoff point of glioma-to-arcuate fasciculus distance for poor outcome was 3.2 mm. These findings suggest that intraoperative magnetic resonance imaging combined with diffusion tensor imaging of the arcuate fasciculus can help optimize tumor resection and result in the least damage to the arcuate fasciculus. Notably, glioma-to-arcuate fasciculus distance is a key independent risk factor for poor postoperative language outcome. This study was approved by the Ethics Committee of the Chinese PLA General Hospital, China (approval No. S2014-096-01) on October 11, 2014.

2.
J Ethnopharmacol ; 265: 113228, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32777517

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The uses of medicinal plants have a long history and become one of the important sources of the health cares in Gaomi City, Shandong Province, China. However, limited studies have been done to identify these medicinal plant species and to scientifically document their associated traditional knowledge. Many species used by indigenous people could potentially represent a novel resource of medicine. The study can aid in further investigations of modern pharmacology and planning of the wild species conservation. AIM OF THE STUDY: The study aimed to investigate and record the medicinal plant taxa and their associated traditional knowledge in Gaomi City, China. MATERIALS AND METHODS: Field study was conducted from March 2018 to May 2019 with 184 residents of Gaomi City. Traditional medicinal plant specimens were collected from the field with the help of these residents and were identified and authenticated in the Herbarium of the School of Pharmacy, Binzhou Medical University. Ethnobotanical knowledge was collected by semi-structured face-to-face interviews. The quantitative data were analyzed by using the informant consensus factor (ICF) method and the number of citations. RESULTS: A total of 181 species belonging to 137 genera and 65 families were collected in Gaomi City. Asteraceae was the predominant family and Fabaceae took the second place. River basins and the southern hills in Gaomi were rich in vegetation. However, the cultivated area of medicinal plants only accounted for 10% of agricultural acreage. The main preparation method was decocting (170, 94.48%) and the most frequent mode of administration was oral (177, 97.97%). The highest numerical ICF value was recorded for treating endocrine, metabolic, and nutritional (ICF: 0.85) conditions. Seven of the medicinal plant species used by the people in Gaomi have not been reported previously in China. Verbena officinalis L. was found in Gaomi City, which is a new distribution record for this species. CONCLUSIONS: People in Gaomi hold valuable knowledge about the use of medicinal plants; however, their knowledge has not been comprehensively documented. The therapeutic uses of the documented medicinal plants will provide a basis for further pharmacological and phytochemical investigations. Additionally, the result of this study indicated that the elder people in Gaomi have more traditional knowledge of plant medicines than the younger ones.

3.
Mol Med Rep ; 23(1)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33179090

RESUMO

Apelin­36 is able to mediate a range of effects on various diseases, and is upregulated in lipopolysaccharide (LPS)­induced acute lung injury (ALI). However, to the best of our knowledge, whether apelin­36 is able to regulate LPS­induced ALI has yet to be investigated. The present study aimed to investigate the role of apelin­36 in LPS­induced ALI, and the putative underlying mechanisms. Rats were assigned to one of four treatment groups: The Control group, apelin­36 group, LPS group and LPS + apelin­36 group. At 4 h after intratracheal instillation of LPS (5 mg/kg), rats were intraperitoneally treated with 10 nmol/kg apelin­36. Subsequently, pathological manifestations and the extent of inflammation and apoptosis of the lung tissues were assessed. Untransfected and apoptosis signal­regulating kinase 1 (ASK1)­overexpressing Beas­2B cells were treated with LPS in the absence or presence of apelin­36, and subsequently the levels of inflammation and apoptosis were assessed. The results obtained showed that the level of apelin­36 was increased in the bronchoalveolar lavage fluid (BALF) of LPS­treated rats. Co­treatment with apelin­36 alleviated LPS­induced lung injury and pulmonary edema, reduced the levels of pro­inflammatory cytokines, including interleukin­6, monocyte chemoattractant protein­1 and tumor necrosis factor­α, in BALF, and inhibited apoptosis in the lung tissues. The presence of apelin­36 also blocked the activation of LPS­induced ASK1, p38, c­Jun N­terminal kinase and extracellular signal­regulated kinase in lung tissues. In vitro studies performed with Beas­2B cells showed that the addition of apelin­36 led to an increase in the cell viability of LPS­induced Beas­2B cells in a concentration­dependent manner. Additionally, co­treatment with 1 µM apelin­36 prevented LPS­induced inflammation and apoptosis. However, overexpression of ASK1 significantly reversed the inhibitory effects of apelin­36 on LPS­induced inflammation and apoptosis. Taken together, the results of the present study demonstrated that apelin­36 was able to protect against LPS­induced lung injury both in vivo and in vitro, and these actions may be dependent on inhibition of the ASK1/mitogen­activated protein kinase signaling pathway.

4.
J Hazard Mater ; 401: 123444, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-32763719

RESUMO

Hydrothermal instability restricts performances of silica-based catalysts, which have wide applications in both industry and environment. For the first time, plasma-thermal slag was revealed to be a catalyst with a born hydrothermal stability in selective catalytic reduction of nitric oxide. The slag catalyst removed 98.5 % of NO with a high N2 selectivity (> 95 %) at 200 °C. After a hydrothermal treatment at 900 °C, the activity of the slag only decreased to 84.0 %. According to characterizations of XRD, HTREM, XPS, and EPR, active metals existed in coordination states in the slag at first. Under hydrothermal conditions, these species transformed to short-range single crystals, which were hindered from sintering by surrounded Si-O bands. At the same time, in-situ DRIFT indicated that more Brønsted and Lewis acid sites were formed. Hence, enough active sties were reserved for effective catalytic reduction of nitric oxide. The main result of this work helps us to understand hydrothermal stability of a catalyst. What's more, the high-value-added utilization of plasma-thermal slag is in favor of the development of hazardous-waste treatment.

5.
J Cell Physiol ; 236(2): 931-945, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32583428

RESUMO

Ischemia reperfusion (I/R)-induced acute kidney injury (AKI) is a common and serious condition. Irisin, an exercise-induced hormone, improves mitochondrial function and reduces reactive oxygen species (ROS) production. Glutathione peroxidase 4 (GPX4) is a key regulator of ferroptosis and its inactivation aggravates renal I/R injury by inducing ROS production. However, the effect of irisin on GPX4 and I/R-induced AKI is still unknown. To study this, male adult mice were subjected to renal I/R by occluding bilateral renal hilum for 30 min, which was followed by 24 hr reperfusion. Our results showed serum irisin levels were decreased in renal I/R mice. Irisin (250 µg/kg) treatment alleviated renal injury, downregulated inflammatory response, improved mitochondrial function, and reduced ER stress and oxidative stress after renal I/R, which were associated with upregulation of GPX4. Treated with RSL3 (a GPX4 inhibitor) abolished irisin's protective effect. Thus, irisin attenuates I/R-induced AKI through upregulating GPX4.

6.
PLoS Negl Trop Dis ; 14(11): e0008873, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-33253321

RESUMO

Bungarus multicinctus is the most venomous snake distributed in China and neighboring countries of Myanmar, Laos, north Vietnam and Thailand. The high mortality rate of B. multicinctus envenomation is attributed to the lethal components of α-, ß-, γ- and κ- bungarotoxins contained in the venom. Although anti-B. multicinctus sera were produced in Shanghai, Taiwan and Vietnam, the most widely clinic used product was term as B. multicinctus antivenin and manufactured by Shanghai Serum Bio-technology Co. Ltd. In the present investigation, high purity α-, ß- and γ-bungarotoxins were separately isolated from B. multicinctus crude venom. Rabbit anti- α-, ß- and γ-bungarotoxin antisera were prepared by common methods, respectively. LD50 values of α-, ß- and γ-bungarotoxins were systematically determined via three administration pathways (intraperitoneal, intramuscular and intravenous injections) in Kunming mice. LD50 values of ß-bungarotoxin were closely related with injection routines but those of both α- and γ-bungarotoxins were not dependent on the injection routines. Commercial B. multicinctus antivenin showed strong immunoreaction with high molecular weight fractions of the B. multicinctus but weakly recognized low molecular weight fractions like α- and γ-bungarotoxins. Although B. multicinctus antivenin showed immunoreaction with high molecular weight fractions of Bungarus fasciatus, Naja atra, Ophiophagus hannah venoms but the antivenin only demonstrated animal protection efficacy against O. hannah venom. These results indicated that the high molecular weight fractions of the O. hannah played an important role in venom lethality but those of B. fasciatus and N. atra did not have such a role.

7.
Clin Lab ; 66(11)2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33180427

RESUMO

BACKGROUND: Chest CT is widely used in clinical diagnosis and efficacy evaluation of CAP. While repeated chest CT examinations to evaluate dynamic changes in chest CT images in a short period of time is a common phenomenon, it causes a lot of waste of medical resources, and due to the large dose of CT radiation, it can cause some harm to the human body. The purpose of this study is to establish a new model to predict the dynamic chest CT image changes of CAP patients by analyzing the age, smoking history, and serum inflammatory markers. METHODS: This is a retrospective study. All patients had received chest CT scan and serum inflammatory indexes were measured, including procalcitonin (PCT), high-sensitivity C-reactive protein (hs-CRP), white blood cell (WBC) and erythrocyte sedimentation rate (ESR). The second chest CT examination was performed after a week of treatment. General information on the medical record was also recorded (including age, smoking history, drinking history, and others). Main outcome measures were the changes of chest CT images, including absorption and non-absorption (including patients with progressive inflammation). Single factor analysis and two-dimensional logistic regression analysis were used to explore the independent risk factors of the new CT image change prediction model for CAP patients. ROC was used to evaluate the sensitivity and specificity of the new model. RESULTS: Among 220 patients with CAP, 150 patients had absorption in chest CT after a week of treatment (150/220), the remaining 70 patients had no absorption or even progression (70/220). Age, PCT, and smoking history were independent risk factors for inflammatory absorption. The AUC of ROC curve was 0.89 (95% CI 0.83 - 0.94), the sensitivity was 88.70%, and the specificity was 80.00%. CONCLUSIONS: A new prediction model consists of serum PCT, age, and smoking history has high specificity and sensitivity in predicting dynamic CT changes in adult CAP patients.

8.
J Neuroeng Rehabil ; 17(1): 150, 2020 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-33187532

RESUMO

BACKGROUND: Virtual reality (VR) has been broadly applied in post-stroke rehabilitation. However, studies on occupational performance and self-efficacy as primary outcomes of stroke rehabilitation using VR are lacking. Thus, this study aims to investigate the effects of VR training on occupational performance and self-efficacy in patients with stroke. METHODS: This was an assessor-blinded, randomized controlled trial. Sixty participants with first-ever stroke (< 1-year onset) underwent rehabilitation in a single acute hospital. Participants were randomly assigned to either the VR group (n = 30) or control group (n = 30). Both groups received dose-matched conventional rehabilitation (i.e., 45 min, five times per week over 3 weeks). The VR group received additional 45-min VR training for five weekdays over 3 weeks. The primary outcome measures were the Canadian Occupational Performance Measure and Stroke Self-Efficacy Questionnaire. Secondary outcome measures included Modified Barthel Index, Fugl-Meyer Assessment-Upper Extremity, and Functional Test for the Hemiplegic Upper Extremity. The assessment was conducted at baseline and after the 3-week intervention. RESULTS: A total of 52 participants (86.7%) completed the trial. Significant between-group differences in Stroke Self-Efficacy Questionnaire (Median Difference = 8, P = 0.043) and Modified Barthel Index (Median Difference = 10, P = 0.030) were found; however, no significant between-group differences in Canadian Occupational Performance Measure, Fugl-Meyer Assessment-Upper Extremity, and Functional Test for the Hemiplegic Upper Extremity were noted. No serious adverse reactions related to the program were reported. CONCLUSIONS: Additional VR training could help improve the self-efficacy and activities of daily living of patients with stroke; however, it was not superior to conventional training in the improvement of upper limb functions, occupational performance, and satisfaction. Nevertheless, VR could be integrated into conventional rehabilitation programs to enhance self-efficacy of patients after stroke. TRIAL REGISTRATION: This study was successfully registered under the title "Effects of virtual reality training on occupational performance and self-efficacy of patients with stroke" on October 13 2019 and could be located in https://www.chictr.org with the study identifier ChiCTR1900026550.

9.
Mol Microbiol ; 2020 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-33231899

RESUMO

Caseinolytic proteases (Clp) are central to bacterial proteolysis and control cellular physiology and stress responses. They are composed of a double-ring compartmentalized peptidase (ClpP) and a AAA+ unfoldase (ClpX or ClpA/ClpC). Unlike many bacteria, the opportunistic pathogen P. aeruginosa contains two ClpP homologs: ClpP1 and ClpP2. The specific functions of these homologs, however, are largely elusive. Here, we report that the active form of PaClpP2 is a part of a heteromeric PaClpP17 P27 tetradecamer that is required for proper biofilm development. PaClpP114 and PaClpP17 P27 complexes exhibit distinct peptide cleavage specificities and interact differentially with P. aeruginosa ClpX and ClpA. Crystal structures reveal that PaClpP2 has non-canonical features in its N- and C-terminal regions that explain its poor interaction with unfoldases. However, experiments in vivo indicate that the PaClpP2 peptidase active site uniquely contributes to biofilm development. These data strongly suggest that the specificity of different classes of ClpP peptidase subunits contributes to the biological outcome of proteolysis. This specialized role of PaClpP2 highlights it as an attractive target for developing antimicrobial agents that interfere specifically with late-stage P. aeruginosa development.

10.
Sci Adv ; 6(48)2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33246955

RESUMO

Genetic signatures caused by demographic and adaptive processes during past climatic shifts can inform predictions of species' responses to anthropogenic climate change. To identify these signatures in Acropora tenuis, a reef-building coral threatened by global warming, we first assembled the genome from long reads and then used shallow whole-genome resequencing of 150 colonies from the central inshore Great Barrier Reef to inform population genomic analyses. We identify population structure in the host that reflects a Pleistocene split, whereas photosymbiont differences between reefs most likely reflect contemporary (Holocene) conditions. Signatures of selection in the host were associated with genes linked to diverse processes including osmotic regulation, skeletal development, and the establishment and maintenance of symbiosis. Our results suggest that adaptation to post-glacial climate change in A. tenuis has involved selection on many genes, while differences in symbiont specificity between reefs appear to be unrelated to host population structure.

11.
J Hazard Mater ; : 124529, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-33218908

RESUMO

Widespread Fe(III)-humic acid (HA) coprecipitates (FHCs) have substantial impacts on the adsorption and reduction of Cr(VI) in soils and sediments, but whether this process is equal to the sum of their individual components remains unknown. In this study, ferrihydrite (Fh)- and HA-like FHCs (C/Fe<3 and C/Fe>3, respectively) were synthesized by controlling the initial C/Fe ratios (0.5-18) to explore the potential synergistic/antagonistic effects during the adsorption and reduction of Cr(VI). According to the results, antagonistic effects on Cr(VI) adsorption (5%-80%) were observed on Fh- and HA-like FHCs, where the antagonistic intensity increased with increasing HA proportions, respectively caused by the more serious occupation of adsorption sites and the stronger electrostatic repulsion to Cr(VI). Notably, significant synergistic reduction effects (5%-650%) occurred on Fh-like FHCs were found to be achieved by the activation of low-molecular HA (0.1-0.3 kDa) with primary/secondary hydroxylic groups, which might be induced by the inductive effect of Fh on complexed HA molecules according to density-functional theory (DFT) calculation. While slight antagonistic reduction effects (2%-45%) by HA-like FHCs were attributed to the decreasing accessibility of Cr(VI) to reductive phenolic groups, which might be blocked within FHC particles or complexed with Fe(III) ions through cation bridges.

12.
Proteins ; 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33210347

RESUMO

Cobaltochelatase in aerobic cobalamin biosynthesis is a complex composed of three subunits. The large subunit CobN is a 140-kDa protein and is homologous to the ChlH subunit of magnesium chelatase. Previously we have reported the 2.5-Å structure of a cyanobacterial ChlH. Here we present the 1.8-Å structure of CobN from Mycobacterium tuberculosis. The overall structure of CobN and ChlH is similar, but significant difference occurs in the head domain. Structural comparison of domains between the two proteins unravels candidate regions for substrate binding and helps to locate a triad of residues that may be essential for metal ion binding.

13.
Sci Rep ; 10(1): 20556, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33239739

RESUMO

Senescence is a degenerative process triggered by intricate and coordinated regulatory networks, and the mechanisms of age-dependent senescence and stress-induced premature senescence still remain largely elusive. Thus we selected leaf samples of developmental senescence (DS) and premature senescence (PS) to reveal the regulatory divergence. Senescent leaves were confirmed by yellowing symptom and physiological measurement. A total of 1171 and 309 genes (DEGs) were significantly expressed respectively in the whole process of DS and PS. Up-regulated DEGs in PS were mostly related to ion transport, while the down-regulated DEGs were mainly associated with oxidoreductase activity and sesquiterpenoid and triterpenoid biosynthesis. In DS, photosynthesis, precursor metabolites and energy, protein processing in endoplasmic reticulum, flavonoid biosynthesis were notable. Moreover, we found the vital pathways shared by DS and PS, of which the DEGs were analyzed further via protein-protein interaction (PPI) network analysis to explore the alteration responding to two types of senescence. In addition, plant hormone transduction pathway was mapped by related DEGs, suggesting that ABA and ethylene signaling played pivotal roles in formulating the distinction of DS and PS. Finally, we conducted a model containing oxidative stress and ABA signaling as two hub points, which highlighted the major difference and predicted the possible mechanism under DS and PS. This work gained new insight into molecular divergence of developmental senescence and premature senescence and would provide reference on potential mechanism initiating and motivating senescence for further study.

14.
J Dermatol ; 2020 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-33222230

RESUMO

Hypotrichosis simplex (HS) is a rare form of hereditary alopecia caused by a variety of genetic mutations. Currently, only four studies regarding LSS-related HS have been reported. In this study, we try to make a definite diagnosis in two unrelated Chinese families with three pediatric patients clinically suspected of HS. Whole-exome sequencing (WES) was performed for these two families to reveal the pathogenic mutation. WES revealed two different compound heterozygous mutations in LSS in two probands that confirmed the diagnosis, including three novel mutations. In this paper, we describe a new accompanying phenotype of teeth dysplasia in a HS patient. Moreover, we provide a review of all reported LSS mutation-related patients and infer some potential genotype-phenotype correlations for the first time.

15.
J Phys Chem Lett ; : 10323-10328, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33227199

RESUMO

In hybrid metal halide perovskites, electrons carry both orbital and spin momenta through s-p wave function hybridization. This leads to a hypothesis that the orbit-orbit interaction between excitons can occur through orbital magnetic dipoles forming short-range interaction or through orbital polarizations forming long-range interaction to influence optoelectronic properties. This Letter reports an interesting phenomenon: the orbit-orbit interaction can be electrically switched between orbital magnetic dipoles and orbital polarizations in a flexible perovskite (MAPbI3-xClx) solar cell by scanning an external voltage between forward and reverse biases (0.2 and -0.2 V). Essentially, this phenomenon presents an external mechanism for electrically controlling the internal orbit-orbit interaction in hybrid perovskites. It was further observed that this bias-switchable orbit-orbit interaction is sensitive to temperature, becoming negligible when the temperature is decreased from 300 to 250 K. This observation indicates that the mobile ions driven by an external electrical field provide an intrinsic mechanism for electrically switching the orbit-orbit interaction through polarization and spin parameters while applying an external voltage between forward and reverse biases. These results provide a comprehensive understanding of tuning the orbit-orbit interaction in flexible perovskites toward developing orbitronic actions.

16.
Food Chem ; : 128498, 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33229156

RESUMO

The acquisition of resveratrol from Polygonum cuspidatum is complicated and costs organic solvents due to extraction and hydrolysis of its corresponding glycoside (polydatin). In this work, a novel one-pot method based on deep eutectic solvent (DES) was developed for simultaneous extraction and conversion of polydatin to resveratrol from Polygonum cuspidatum for the first time. The extraction yield of resveratrol by DES-based one-pot method were significantly higher than that of water, methanol and ethanol. After optimization by One-Variable-at-a-Time and response surface methodology, the extraction yield of resveratrol reached 12.26 ± 0.14 mg/g within 80 min. The conversation efficiency of polydatin to resveratrol in Polygonum cuspidatum from five different origins was more than 96.3%. Scanning electron microscope results indicated the selected DES disrupted plant cell walls to enhance the yield of resveratrol. The results indicated that one green method was successfully established for efficient extraction and conversion of polydatin to resveratrol from Polygonum cuspidatum.

17.
Clin Psychol Rev ; 83: 101932, 2020 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-33176244

RESUMO

To evaluate the global prevalence of antenatal depression and clarify its potential associated factors, we conducted two systematic reviews and meta-analyses, where appropriate. PubMed, Web of Science, and Embase were used to identify studies published up to Feb 28, 2019. The pooled prevalence of any antenatal depression across 173 studies with 182 reports was 20.7% (95% CI 19.4-21.9%, P = 0.000, I2 = 98.4%), and the pooled prevalence of major antenatal depression across 72 studies with 79 reports was 15.0% (95% CI 13.6-16.3%, P = 0.000, I2 = 97.8%). The prevalence of antenatal depression was higher in low- or lower-middle-income countries, and in studies using self-report instruments or conducted after the year 2010. History of depression, lack of social support, single/separated/divorced status, unplanned pregnancy, unemployment, experience of violence, and smoking before or during pregnancy were significantly associated with antenatal depression. The results of our study indicated that a significant number of pregnant women experience depression and verified some factors that are related to this disorder. As countermeasures, it is important to develop effective risk assessment strategies as well as prevention and intervention strategies for antenatal depression based on its associated factors.

19.
Pain Physician ; 23(6): 541-551, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33185370

RESUMO

BACKGROUND: Postherpetic neuralgia (PHN) is a neuropathic pain that causes a reduction in patients' quality of life. There are many topical drugs for PHN, including topical lidocaine patch, topical application of capsaicin, and others. OBJECTIVES: This study aims to compare the efficacy and safety of topical drugs for PHN. STUDY DESIGN: Relevant studies were found by systemically searching for terms including "topical" and "Postherpetic neuralgia" in PubMed, Cochrane library, MEDLINE, and EMBASE databases (inception through June 12, 2019). The primary outcome was the percentage of change in the Numeric Rating Scale or the Visual Analog Scale scores from baseline. The secondary outcome was the number of adverse events. METHODS: The efficacy and safety of topical drugs for PHN was investigated by the pairwise meta-analysis and Bayesian network meta-analysis, applying Revman 5.3, the Stata 14.0 software, and GeMTC 0.14.3. RESULTS: Twelve studies met the inclusion criteria, and eligible studies were selected for the ultimate meta-analysis. Our meta-analysis displayed 6 topical drugs for PHN. Lidocaine, high-concentration capsaicin, and aspirin/diethyl ether (ADE) had a higher possibility of bringing pain relief than placebo. Among them, lidocaine had the highest possibility of being the most effective drug for PHN and had the statistical significances compared with diclofenac, high-concentration capsaicin, indomethacin, low-concentration capsaicin, and placebo, and lidocaine was significantly preferable than other effective drugs in the aspect of safety. LIMITATIONS: (1) The small number of included studies; (2) a small number of patients and short-term trials in progress, including lidocaine and ADE; (3) both randomized controlled trial and crossover randomized trial were included in our network meta-analysis; (4) only studies published in English were evaluated; (5) lack of head-to-head comparisons of some treatments; (6) different measurement methods were used in different trial, which may cause deviation; and (7) with the lack of cycles in the included trials, the inconsistency factors cannot be calculated, and node-splitting method cannot be performed in our network meta-analysis to check the inconsistency. CONCLUSIONS: Compared with other topical drugs, lidocaine was the most effective and most tolerable drug to be recommended for PHN.

20.
J Dairy Sci ; 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33162072

RESUMO

The fat content of milk determines the quality of milk, and triglycerides are the major components of milk fat. Milk fat synthesis is regulated by many factors. Lipopolysaccharide (LPS) has been shown to inhibit milk fat synthesis in bovine mammary epithelial cells, but research on the underlying mechanisms has been limited. MicroRNA (miRNA) are involved in many physiological processes, but there have been few studies on their regulation in milk fat synthesis. In this study, we aimed to investigate whether LPS upregulates miR-27a-3p, which targets PPARγ, thereby inhibiting the synthesis of triglycerides in a dairy cow mammary epithelial cell line (MAC-T). After LPS stimulation of MAC-T cells, PPARγ gene expression and milk fat synthesis were inhibited. TargetScan software was used to predict miRNA targeting PPARγ, and miR-27a-3p was selected as a candidate. A dual luciferase reporter assay further confirmed the targeting connection between miR-27a-3p and the PPARγ gene. To investigate the functions of miR-27a-3p, miR-27a-3p mimic and inhibitors were transfected into MAC-T cells. The mRNA and protein levels of PPAR-γ were negatively correlated with the expression of miR-27a-3p. Lipid droplet accumulation and triglyceride synthesis were also negatively correlated with miR-27a-3p expression. Inhibition of miR-27a-3p partially reversed the LPS-induced decreases in PPARγ expression and milk fat synthesis. In summary, our results reveal that LPS can inhibit MAC-T cell milk fat synthesis by upregulating miR-27a-3p, which targets the PPARγ gene.

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