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1.
Nat Methods ; 2020 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-33288960

RESUMO

Circular RNAs (circRNAs) produced from back-spliced exons are widely expressed, but individual circRNA functions remain poorly understood owing to the lack of adequate methods for distinguishing circRNAs from cognate messenger RNAs with overlapping exons. Here, we report that CRISPR-RfxCas13d can effectively discriminate circRNAs from mRNAs by using guide RNAs targeting sequences spanning back-splicing junction (BSJ) sites featured in RNA circles. Using a lentiviral library that targets sequences across BSJ sites of highly expressed human circRNAs, we show that a group of circRNAs are important for cell growth mostly in a cell-type-specific manner and that a common oncogenic circRNA, circFAM120A, promotes cell proliferation by preventing the mRNA for family with sequence similarity 120A (FAM120A) from binding the translation inhibitor IGF2BP2. Further application of RfxCas13d-BSJ-gRNA screening has uncovered circMan1a2, which has regulatory potential in mouse embryo preimplantation development. Together, these results establish CRISPR-RfxCas13d as a useful tool for the discovery and functional study of circRNAs at both individual and large-scale levels.

2.
J Dermatol ; 2020 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-33264455

RESUMO

Chronic actinic dermatitis (CAD) is a common debilitating photodermatosis. Patients often have to completely avoid outdoor activities, which severely impacts their quality of life. Phototherapy is effective for CAD and seems to increase patients' tolerance towards sunlight and consequently decrease the extent of disease. Unfortunately, the slower onset and time-consuming nature of phototherapy limits the clinical application. Considering the effectiveness and time-saving nature of ultraviolet (UV)-A rush hardening in solar urticaria, we performed a pilot study to determine whether UV-A rush hardening is effective in CAD. Six patients with CAD were exposed to multiple sessions of UV-A for 4-5 days at 1-h intervals/day. Subsequently, maintenance UV-A exposure was performed at 1-2-week intervals. Phototesting at baseline showed that three patients were sensitive to both UV-A and -B, and the other three patients only showed UV-A sensitivity. All of the patients responded well to UV-A rush hardening and four (67%) maintained a good remission status after 1 year. The results of this pilot study suggest that UV-A rush hardening phototherapy is effective and well tolerated in the treatment of CAD, while future larger prospective studies using objective scores of disease activity and quality of life are needed.

3.
Front Endocrinol (Lausanne) ; 11: 587450, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33363516

RESUMO

Islet culture prior to transplantation is a standard practice in many transplantation centers. Nevertheless, the abundant islet mass loss and function impairment during this serum-deprivation culture period restrain the success of islet transplantation. In the present study, we used a natural biomaterial derived product, amniotic membrane extract (AME), as medium supplementation of islet pretransplant cultivation to investigate its protective effect on islet survival and function and its underlying mechanisms, as well as the engraftment outcome of islets following AME treatment. Results showed that AME supplementation improved islet viability and function, and decreased islet apoptosis and islet loss during serum-deprived culture. This was associated with the increased phosphorylation of PI3K/Akt and MAPK/ERK signaling pathway. Moreover, transplantation of serum-deprivation stressed islets that were pre-treated with AME into diabetic mice revealed better blood glucose control and improved islet graft survival. In conclusion, AME could improve islet survival and function in vivo and in vitro, and was at least partially through increasing phosphorylation of PI3K/Akt and MAPK/ERK signaling pathway.

4.
ACS Appl Mater Interfaces ; 12(47): 53247-53256, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33185423

RESUMO

Conductive hydrogels have shown great potential in the field of flexible strain sensors. However, their application is greatly limited due to the low conductivity and poor mechanical properties at subzero temperatures. Herein, an ultrastretchable, tough, antifreezing, and conductive cellulose hydrogel was fabricated by grafting acrylonitrile and acrylamide copolymers onto the cellulose chains in the presence of zinc chloride using ceric ammonium nitrate as the initiator. The resulting hydrogel exhibited ultrastretchability (1730%), excellent tensile strength (160 kPa), high elasticity (90%), good toughness (1074.7 kJ/m3), and fatigue resistance property due to the existence of dipole-dipole and multiple hydrogen-bonding interactions on the hydrogel network. In addition, the introduced zinc chloride endowed the cellulose-based hydrogel with remarkable electric conductivity (1.54 S/m) and excellent antifreezing performance (-33 °C). Finally, the hydrogel showed high sensitivity and stability to monitor human activities. In summary, this work presented a facile strategy to construct conductive hydrogel with excellent antifreezing and mechanical properties simultaneously, which showed great potential for wearable strain sensors.

5.
Fish Shellfish Immunol ; 107(Pt B): 444-451, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33160021

RESUMO

Poly-ß-hydroxybutyrate (PHB) can be hydrolyzed to ß-hydroxybutyrate (ß-HB) in the intestinal tract of animals, and dietary PHB supplementation could enhance the immunity and disease resistance of aquatic animals. Antioxidant system is responsive to PHB stimuli via MAPK/PI3K-Akt/TNF/NF-κB/TCR/TLR signaling pathways. However, the precise immunopotentiation mechanism needs further study. In this study, macrophages from spleen in Liza haematocheila was used to study the effect of ß-HB on cell viability and antioxidant function to illustrate the immunopotentiation mechanism of PHB. The results showed that ß-HB (100 µg/mL) promoted the viability of macrophages and balanced the production of reactive oxygen species, but inhibited the excessive production of intracellular nitric oxide. In order to further explore the immunopotentiation mechanism of ß-HB, LPS (100 µg/mL) was used to induce the inflammation and investigated the inhibitory effect of ß-HB on inflammation. The results showed that LPS could induce inflammation successfully, and ß-HB exerted anti-inflammatory and antioxidant effects in LPS-stimulated macrophages. Compared with LPS stimuli alone, the expression of anti-inflammatory genes NF-κBIA, MAP3K8 and TLR5 in ß-HB pretreatment group was up-regulated, and the expression of pro-inflammatory genes TNFSF6, TNF-α, PI3K, NF-κB and TLR1 down-regulated. It suggested that ß-HB inhibited the inflammatory response by up-regulation of anti-inflammatory genes such as NF-κBIA, thereby enhancing the immunity of the body.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33137406

RESUMO

BACKGROUND: Individuals with internet gaming disorder (IGD) usually report a higher sense of loneliness. Although studies have suggested a key role of the prefrontal cortex-based resting-state functional connectivity (rsFC) in both IGD and loneliness, the potential mechanism between IGD and loneliness remains unclear. METHODS: Fifty-seven IGD and 81 matched recreational internet gamer users (RGU) underwent resting-state fMRI scans. The UCLA loneliness scale was used to measure loneliness. We first explored the brain areas that are both associated with loneliness and IGD severity. Then, the neuroimaging findings were extracted to test whether the rsFC of these brain regions moderates the relationship between IGD and loneliness. RESULTS: We observed reduced rsFC between the left dorsolateral prefrontal cortex (DLPFC) and the left precentral and the postcentral gyri and the supplementary motor area (SMA), which also correlated with increased IAT (Young''s internet addiction test) scores. More importantly, the rsFC of the DLPFC-precentral gyrus and the DLPFC-postcentral gyrus moderated the relationship between IGD severity and loneliness scores. Additionally, we also found that the rsFC of the left DLPFC-precentral gyrus, the DLPFC-postcentral gyrus and the right DLPFC-SMA moderated the relationship between self-reported gaming craving and the UCLA scores. CONCLUSIONS: The current study confirmed the role of the DLPFC in reward control (game craving) and emotion regulation (loneliness). Additionally, the rsFC of the prefrontal cortex-supplementary motor area moderates IGD and loneliness. These findings provide valuable understanding of the two-way relationship between IGD and loneliness.

7.
Food Res Int ; 137: 109714, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33233286

RESUMO

In this paper, we report a newly developed non-target 1H NMR detection associated with chemometrics method to classify the botanical and geographical origins of the monofloral Chinese honey. 1H NMR tests of 218 monofloral honey samples of 8 classes (Acacia, Jujube, Linden, Longan, Orange, Rape, Sunflower, Vitex) collected in 2017-2019 across China were conducted under the optimal sample preparation conditions and NMR acquisition parameters. The whole profiles of NMR spectra instead of individual or partial signals from specific components were processed and extracted, then fed to SIMCA-P to classify the botanical and geographical origins through non-target statistical analysis. For the botanical origins, most of them could be classified clearly according to Principal Component Analysis (PCA) with both R2 and Q2 close to 1. Orthogonal Partial Least Squares Discrimination Analysis (OPLS-DA) model could classify the honey floral types successfully with R2Y and Q2 greater than 0.85. It is found that the integral bin for data extraction has no obvious influence on the classification. For the geographical origins, the classification at different geographical levels (providence and town) could be successfully distinguished by OPLS-DA model. The promising preliminary results with the geographical classification at 40 km level unambiguously demonstrate the application of this NMR-based multi-species non-targeted method for the honey authenticity. Successful result is obtained on a pilot prediction of the geographical classification. Comparing with the methods based on other techniques, the advantages of this reported one are less sample amount needed, simple preparation, short test time, and non-targeted multi-species detection.

8.
Addict Biol ; : e12985, 2020 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-33236526

RESUMO

Cognitive, functional, and structural brain factors involving frontal executive and striatal reward networks have been implicated in Internet gaming disorder (IGD). However, frontostriatal network connectivity and its association with addiction severity are poorly understood in IGD. Resting-state fMRI data from 337 subjects (130 with IGD, 207 with recreational game use [RGU]) were collected. Striatal-cortical communications were measured with resting-state functional connectivity (FC) using coherent spontaneous fluctuations in the blood-oxygenation-level-dependent fMRI signal. Correlations were calculated between FC measures and IGD-related assessments (addiction severity and craving scores). Decreased FC was predominantly observed in IGD subjects, with IGD subjects showing decreased FC between the putamen and superior frontal gyrus (SFG), middle frontal gyrus (MFG), and inferior frontal gyrus (IFG) and the ventral striatum and IFG, superior temporal gyrus, and MFG. Disorder severity and craving scores were negatively correlated with FC between striatal and frontal brain regions. Associations between diminished FC in corticostriatal circuitry and clinical features (IGD craving, severity) suggest potential therapeutic targets for neuromodulation treatments. The extent to which frontostriatal circuits involving executive control over reward processes may be altered to treat IGD warrants additional study.

9.
J Behav Addict ; 9(3): 642-653, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33031057

RESUMO

Background: Internet gaming disorder (IGD) is included in the DSM-5 as a provisional diagnosis. Whether IGD should be regarded as a disorder and, if so, how it should be defined and thresholded have generated considerable debate. Methods: In the current study, machine learning was used, based on regional and interregional brain features. Resting-state data from 374 subjects (including 148 IGD subjects with DSM-5 scores ≥5 and 93 IGD subjects with DSM-5 scores ≥6) were collected, and multivariate pattern analysis (MVPA) was employed to classify IGD from recreational game use (RGU) subjects based on regional brain features (ReHo) and communication between brain regions (functional connectivity; FC). Permutation tests were used to assess classifier performance. Results: The results demonstrated that when using DSM-5 scores ≥5 as the inclusion criteria for IGD subjects, MVPA could not differentiate IGD subjects from RGU, whether based on ReHo or FC features or by using different templates. MVPA could differentiate IGD subjects from RGU better than expected by chance when using DSM-5 scores ≥6 with both ReHo and FC features. The brain regions involved in the default mode network and executive control network and the cerebellum exhibited high discriminative power during classification. Discussion: The current findings challenge the current IGD diagnostic criteria thresholding proposed in the DSM-5, suggesting that more stringent criteria may be needed for diagnosing IGD. The findings suggest that brain regions involved in the default mode network and executive control network relate importantly to the core criteria for IGD.

10.
Front Endocrinol (Lausanne) ; 11: 568864, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33101203

RESUMO

The traditional Chinese medicine has long been used in the treatment of diabetes, one major disease threatening the public health. It has been reported that artemether exerts antidiabetic effects on type 2 diabetes in db/db mice, however the underlying mechanisms remain unknown. In the present study, we show that artemether regulates expression of related enzymes participating in the glucose and lipid metabolism in the liver of db/db mice, which could at least partly explain the improved glucose and lipid metabolism in artemether-treated mice. Additionally, artemether also regulates expression of glycogen synthesis related enzymes in the skeletal muscle of db/db mice, supporting its promotive role in glycogen synthesis. Mechanistically, artemether activates AMPK pathway as well as PI3K/Akt pathway in the liver and skeletal muscle of db/db mice, suggesting that these two signaling pathways are both involved in the antidiabetic effects of artemether on type 2 diabetes in db/db mice. In conclusion, our study connects the antidiabetic effects of artemether to the regulation of metabolic enzymes and signaling pathways, and also provides molecular basis for the potential application of artemether in treating type 2 diabetes.

11.
Brief Bioinform ; 2020 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-32894863

RESUMO

Cancer is a highly heterogeneous disease caused by dysregulation in different cell types and tissues. However, different cancers may share common mechanisms. It is critical to identify decisive genes involved in the development and progression of cancer, and joint analysis of multiple cancers may help to discover overlapping mechanisms among different cancers. In this study, we proposed a fusion feature selection framework attributed to ensemble method named Fisher score and Gradient Boosting Decision Tree (FS-GBDT) to select robust and decisive feature genes in high-dimensional gene expression datasets. Joint analysis of 11 human cancers types was conducted to explore the key feature genes subset of cancer. To verify the efficacy of FS-GBDT, we compared it with four other common feature selection algorithms by Support Vector Machine (SVM) classifier. The algorithm achieved highest indicators, outperforms other four methods. In addition, we performed gene ontology analysis and literature validation of the key gene subset, and this subset were classified into several functional modules. Functional modules can be used as markers of disease to replace single gene which is difficult to be found repeatedly in applications of gene chip, and to study the core mechanisms of cancer.

12.
BMC Genomics ; 21(1): 650, 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32962626

RESUMO

BACKGROUND: The small number of samples and the curse of dimensionality hamper the better application of deep learning techniques for disease classification. Additionally, the performance of clustering-based feature selection algorithms is still far from being satisfactory due to their limitation in using unsupervised learning methods. To enhance interpretability and overcome this problem, we developed a novel feature selection algorithm. In the meantime, complex genomic data brought great challenges for the identification of biomarkers and therapeutic targets. The current some feature selection methods have the problem of low sensitivity and specificity in this field. RESULTS: In this article, we designed a multi-scale clustering-based feature selection algorithm named MCBFS which simultaneously performs feature selection and model learning for genomic data analysis. The experimental results demonstrated that MCBFS is robust and effective by comparing it with seven benchmark and six state-of-the-art supervised methods on eight data sets. The visualization results and the statistical test showed that MCBFS can capture the informative genes and improve the interpretability and visualization of tumor gene expression and single-cell sequencing data. Additionally, we developed a general framework named McbfsNW using gene expression data and protein interaction data to identify robust biomarkers and therapeutic targets for diagnosis and therapy of diseases. The framework incorporates the MCBFS algorithm, network recognition ensemble algorithm and feature selection wrapper. McbfsNW has been applied to the lung adenocarcinoma (LUAD) data sets. The preliminary results demonstrated that higher prediction results can be attained by identified biomarkers on the independent LUAD data set, and we also structured a drug-target network which may be good for LUAD therapy. CONCLUSIONS: The proposed novel feature selection method is robust and effective for gene selection, classification, and visualization. The framework McbfsNW is practical and helpful for the identification of biomarkers and targets on genomic data. It is believed that the same methods and principles are extensible and applicable to other different kinds of data sets.

13.
Nanomaterials (Basel) ; 10(9)2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32882830

RESUMO

A broadband near-perfect absorber is analyzed by an amorphous silicon (a-Si) hook shaped nanostructure metasurface. The transmission and reflection coefficients of the metasurface are investigated in the point electric and magnetic dipole approximation. By combining square and semicircle nanostructures, the effective polarizabilities of the a-Si metasurface calculated based on discrete dipole approximation (DDA) exhibit broadened peaks of electric dipole (ED) and magnetic dipole (MD) Mie resonances. The optical spectra of the metasurface are simulated with different periods, in which suppressed transmission are shifted spectrally to overlap with each other, leading to broadened enhanced absorption induced by interference of ED and MD Mie resonances. The angle insensitive absorption of the metasurface arrives 95% in simulation and 85% in experiment in spectral range from 564 nm to 584 nm, which provides potential applicability in nano-photonic fields of energy harvesting and energy collection.

14.
BMC Gastroenterol ; 20(1): 278, 2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32819277

RESUMO

BACKGROUND: Almost all liver tumours can be removed laparoscopically, but some difficult tumour locations complicate laparoscopic surgery. Recurrent liver tumours often pose great difficulties to laparoscopic surgery due to adhesions caused by previous operations. Referring to laparoscopic adrenalectomy, a retroperitoneal approach is proposed to remove liver tumours near the adrenal gland, which will provide a new method for liver surgery. CASE PRESENTATION: Our case involves a patient with recurrent hepatocellular carcinoma (HCC) whose last operation was laparoscopic hepatectomy in our department, with a recurrence of HCC 2 years after the first surgery. In this case, based on preoperative CT and MRI, through a retroperitoneal approach, combined with intraoperative ultrasound (IOUS) localization and indocyanine green (ICG) fluorescence navigation, laparoscopic hepatectomy was successfully performed to precisely resect recurrent hepatocellular carcinoma in segment VII. The patient was discharged on the third day after the operation. The AFP decreased to normal levels on the 28th postoperative day. CONCLUSIONS: Retroperitoneal hepatectomy has the advantages of less trauma, shorter operation times, fewer complications and faster recovery for hepatic tumours near the adrenal gland. Accurate localization of tumours is needed to ensure accurate resection; therefore, IOUS and ICG fluorescence are very important. Liver parenchyma was severed strictly according to fluorescent labelling during hepatectomy, which prevented the deviation of liver parenchyma from the plane and ensured that the margin of hepatectomy was tumour-free. In order to ensure a radical resection of the tumour, it may be necessary to enter the abdominal cavity.

15.
Front Psychol ; 11: 1605, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32793040

RESUMO

Since the core of talent training in modern society has changed from allowing students to master knowledge to adapting them to a lifelong learning society, this paper studied the innovation education in higher education by combining human comprehensive development theory and deep learning. First, this paper described the training needs of innovative education talents. Second, based on the human comprehensive development theory and deep learning, it puts forward views on innovative quality education in colleges and evaluates the students' deep learning status by designing a deep learning evaluation model. From the perspective of students, a questionnaire for student-side use was designed according to the deep learning evaluation scale so that students could understand their deep learning status, make improvements based on their current situation, and improve the level of deep learning, thereby obtaining the inspiration of innovation education in higher education. The research shows that the students' mastery of knowledge and skills is the highest, indicating that the mastery of students in this respect has reached the standard. Although the mean value obtained by the process and method is greater than the median, it is almost the same as the median, which indicates that the students' mastery of the process and method is still lacking and further efforts are needed. In terms of psychology and values, the mean value is the lowest among the three dimensions and less than the median, indicating that students are the weakest in this respect and further improvement is required. In addition, a new teaching evaluation method was designed based on human comprehensive development theory and deep learning, contributing to innovation education in higher education in China.

16.
Cell Transplant ; 29: 963689720943576, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32731817

RESUMO

Although 90% of clinical islet transplantations are performed via the portal vein approach, it is still far from the ideal transplant site. Alternative islet transplant sites are promising to reduce the islet dose required to reverse hyperglycemia, thereby improving the efficiency of islet transplantation. The aim of this study was to compare the differences in survival and metabolic function of islet grafts transplanted into the hepatic sinus tract (HST) and the splenic parenchyma (SP). Approximately 300 syngeneic mouse islets were transplanted into the HST (n = 6) and the SP (n = 6) of recipient diabetic mice, respectively. After transplantation, the glycemic control, glucose tolerance, and morphology of islet grafts were evaluated and compared in each group. The nonfasting blood glucose of the two groups of mice receiving islet transplantation gradually decreased to the normal range and sustained for more than 100 d. There is no significant difference in the time required to restore normoglycemia (P > 0.05). The results of the glucose tolerance test showed that the SP group presented a smaller area under the curve than the HST group (P < 0.05). Histopathological results showed that islet grafts in the HST and the SP were characterized with normal islet morphology and robust insulin production. Compared with the HST, islet transplantation in the SP presents better blood glucose regulation, although there is no significant difference in the time required to restore normoglycemia.

17.
Opt Lett ; 45(13): 3649-3652, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32630921

RESUMO

The transport-of-intensity equation (TIE) is one of the most well-known approaches for phase retrieval and quantitative phase imaging. It directly recovers the quantitative phase distribution of an optical field by through-focus intensity measurements in a non-interferometric, deterministic manner. Nevertheless, the accuracy and validity of state-of-the-art TIE solvers depend on restrictive pre-knowledge or assumptions, including appropriate boundary conditions, a well-defined closed region, and quasi-uniform in-focus intensity distribution, which, however, cannot be strictly satisfied simultaneously under practical experimental conditions. In this Letter, we propose a universal solution to TIE with the advantages of high accuracy, convergence guarantee, applicability to arbitrarily shaped regions, and simplified implementation and computation. With the "maximum intensity assumption," we first simplify TIE as a standard Poisson equation to get an initial guess of the solution. Then the initial solution is further refined iteratively by solving the same Poisson equation, and thus the instability associated with the division by zero/small intensity values and large intensity variations can be effectively bypassed. Simulations and experiments with arbitrary phase, arbitrary aperture shapes, and nonuniform intensity distributions verify the effectiveness and universality of the proposed method.

18.
BMC Psychiatry ; 20(1): 357, 2020 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-32635911

RESUMO

BACKGROUND: Many studies have found an interesting issue in the Internet gaming disorder (IGD): males are always observed to be the majority. However, there are little research to exploring the differences in the neural mechanisms between males and females in decision-making process among people with IGD. Therefore, explore the reward/loss processing between different gender with IGD could help in understanding the underlying neural mechanism of IGD. METHODS: Data from functional magnetic resonance imaging (fMRI) were collected from 111 subjects (IGD: 29 males, 25 females; recreational internet game user (RGU): 36 males, 21 females) while they were performing a card-guessing task. We collected and compared their brain features when facing the win and loss conditions in different groups. RESULTS: For winning conditions, IGD group showed hypoactivity in the lingual gyrus than RGU group, male players showed hyperactivity in the left caudate nucleus, bilateral cingulate gyrus, right middle frontal gyrus (MFG), right precuneus and inferior parietal lobule relative to the females. And significant sex-by-group interactions results showed higher brain activities in the thalamus, parahippocampal gyrus and lower brain activities in Inferior frontal gyrus (IFG) were observed in males with IGD than females. For losing conditions, IGD group showed hypoactivity in the left lingual gyrus, parahippocampal gyrus and right anterior cingulate cortex (ACC) compared to the RGU group, male players showed hyperactive left caudate nucleus and hypoactive right middle occipital gyrus relative to females. And significant sex-by-group interactions results showed that compared to females with IGD, males with IGD showed decreased brain activities in the IFG and lingual gyrus. CONCLUSIONS: First, there appeared to be no difference in reward processing between the IGD and RGU group, but IGD showed less sensitivity to loss. Secondly, male players showed more sensitivity to rewards and less sensitivity to losses. Last but not least, males and females showed opposite activation patterns in IGD degree and rewards/losses processing. And male IGD subjects are more sensitive to reward and less sensitive to loss than females, which might be the reason for the gender different rates on IGD.

19.
Diabetes Metab Syndr Obes ; 13: 2081-2092, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32606867

RESUMO

Introduction: Yu Nu compound (YNJ) is a traditional Chinese medicine widely utilized to treat type 2 diabetes possibly through mediating autophagy. Abnormal podocyte autophagy and apoptosis could result in podocyte loss in diabetics nephropathy (DN). The mechanism of Yu Nu compound in DN is still unclear. Therefore, the study aims to investigate the effects of Yu Nu compound and analyze the potential mechanism. Methods: Goto-Kakizaki (GK) rats were administered using YNJ with different doses once a day by gavage for 4 weeks. The renal cortex injury was observed by HE staining and electron microscope. Cell apoptosis of renal cortex was analyzed by TUNNEL staining. The mTOR, autophagy-related proteins and apoptosis-related proteins were detected by Western blot or real-time PCR in vivo and vitro. MPC5 cells were exposed to high glucose (HG, 30mM) for 12h to simulate podocyte injury in DN. MPC5 cells were treated by serum containing YNJ with different dosages. Cell activities and apoptosis were, respectively, detected through Cell Counting Kit-8 (CCK8) assay and flow cytometry. Results: The results showed that the medium dose of YNJ had better effects on decreasing blood glucose and improving renal injury in GK rats, followed by decreasing mTOR levels. The autophagy levels were enhanced in renal cortex, accompanied with the increase of cell apoptosis in vivo. Besides, the proteins regulating autophagy and apoptosis were significantly modulated by YNJ in GK rats. Then, we found that the decreasing endogenous mTOR could reverse the effects of YNJ on podocyte apoptosis and autophagy in vivo. Discussion: The study suggested that YNJ recovered normal autophagy and suppressed apoptosis through regulating mTOR. The maintenance of normal basal autophagic activity possibly based on the effect of YNJ on multiple target was essential for maintaining podocyte function.

20.
Materials (Basel) ; 13(14)2020 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-32708632

RESUMO

At present, there are no specific indicators and requirements for the low-temperature crack resistance of emulsified asphalt cold recycled mixture (CRME) in the Chinese road mixture specifications. In order to expand the application of this technology in the asphalt surface layer in cold areas, this paper studied the influence of 10 influencing factors on the low-temperature anti-cracking performance of CRME through the semicircular bending test (SCB) with fracture energy as the evaluation index. The research results show that the fracture energy index of the SCB test can be used to evaluate the low temperature crack resistance of CRME. After 10 kinds of influencing factors were analyzed, it was found that the biggest factor affecting the low-temperature cracking resistance of the mixture was the recycling agent, which had an effect on the fracture energy index of over 60%. Followed by cement, fiber and compaction work, the degree of influence exceeded 30%. Finally, combined with engineering application experience, some specific measures to improve the low-temperature anti-cracking performance of CRME were proposed.

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