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1.
Acta Trop ; : 105285, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31786108

RESUMO

Dipstick Dye Immunoassay (DDIA) and Indirect Haemagglutination Assay(IHA), are two commercially available kits which have been widely used for screening Schistosoma japonicum in P.R. China. Whether they can be used for screening of Schistosoma haematobium are not clear. In order to evaluate the diagnostic efficiency of DDIA and IHA for screening Schistosoma haematobium, serum samples were collected from pupils in endemic areas in Zambia, Southern Africa, and tested by DDIA and IHA by single-blind manner. Meanwhile, the pupils were microscopically examined by infection with Schistosoma and soil-transmitted helminths, visually observed for parasite eggs. Of the enrolled 148 pupils, 61% tested positive for S. haematobium infection, while 31% and 36% of pupils were infected with hookworm and Ascaris respectively. Regarding the parasitological tests as reference standard, for the diagnosis of S. haematobium infection, IHA performed higher sensitivity (74%, 95% CI: 65%-83%) than that of DDIA (60%, 95%CI: 49%-70%). The sensitivities of IHA and DDIA are significant higher in 10-14 years old students than those of 7-9 years old group. The specificity of DDIA and IHA were 61% (95%CI: 49%-74%) and 72% (95%CI: 60%-84%), respectively. The co-infection with STHs decreased the specificity of DDIA but had no impact on that of IHA. Our study indicated that IHA has more potential as an alternative diagnostic tool for identifying schistosomiasis haematobium but need further improvement.

2.
Pain Physician ; 22(6): E635-E647, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31775417

RESUMO

BACKGROUND: Thalamic pain is a neuropathic pain syndrome that occurs as a result of thalamic damage. It is difficult to develop therapeutic interventions for thalamic pain because its mechanism is unclear. To better understand the pathophysiological basis of thalamic pain, we developed and characterized a new rat model of thalamic pain using a technique of microinjecting cobra venom into the ventral posterolateral nucleus (VPL) of the thalamus. OBJECTIVES: This study will establish a new thalamic pain rat model produced by administration of cobra venom to the unilateral ventral posterolateral nucleus. STUDY DESIGN: This study used an experimental design in rats. SETTING: The research took place in the laboratory at the Aviation General Hospital of China Medical University and Beijing Institute of Translational Medicine. METHODS: Male Sprague-Dawley rats were subjected to the administration of cobra venom or saline into the left VPL. The development of mechanical hyperalgesia and changes in pain-related behaviors and motor function were measured after intrathalamic cobra venom microinjection using the von Frey test, video recording, and cylinder test, respectively. On postoperative days 7 to 35, both electroacupuncture and pregabalin (PGB) were administered to verify that the model reproduced the findings in humans. Moreover, the organizational and structural alterations of the thalamus were examined via transmission electron microscopy (TEM). RESULTS: The threshold for mechanical stimuli in the left facial skin was significantly decreased on day 3 after thalamic pain modeling as compared with pre-venom treatment. Furthermore, the ultrastructural alterations of neurons such as indented neuronal nuclei, damaged mitochondria and endoplasmic reticulum, and dissolved surrounding tissues were observed under TEM. Moreover, electroacupuncture treatment ameliorated mechanical hyperalgesia, pain-like behaviors, and motor dysfunction, as well as restore normal structures of neurons in the thalamic pain rat model. However, no such beneficial effects were noted when PGB was administered. LIMITATIONS: The pathophysiological features were different from the present model and the patients in clinical practice (in most cases strokes, either ischemic or hemorrhagic). CONCLUSION: The cobra venom model may provide a reasonable model for investigating the mechanism of thalamic pain and for testing therapies targeting recovery and pain after thalamic lesions. KEY WORDS: Thalamic pain, cobra venom, electroacupuncture, pregabalin, indented neuronal nuclei, damaged mitochondria, dissolved endoplasmic reticulum, golgi body.

3.
Inflammation ; 42(5): 1843-1856, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31256292

RESUMO

Ozone is a strong oxidant in air pollution that exacerbates respiratory disorders and is a major risk factor for acute asthma exacerbation. Ozone can induce reactive oxygen species (ROS) and airway neutrophilic inflammation. In addition, γδT17 cells contribute to IL-17A production upon ozone challenge, resulting in neutrophilic inflammation. It is known, however, that Nrf2 can ameliorate oxidative stress. We therefore investigated whether RTA-408, an Nrf2 activator, can attenuate airway inflammation and inhibit ROS production and whether this effect involves γδT17 cells. Balb/c mice were sensitized/challenged with ovalbumin (OVA) and followed by ozone exposure. We investigated the effect of Nrf2 activator RTA-408 on airway hyperresponsiveness, neutrophilic airway inflammation, cytokine/chemokine production, and OVA-specific IgE level in a mouse model of O3 induced asthma exacerbation. Furthermore, malondialdehyde (MDA) and glutathione (GSH) levels in lung and intracellular ROS were measured. IL-17+ γδT cell percentage by flow cytometer was determined. Nrf2 protein expression by western blot was also examined. We observed that RTA-408 attenuated ROS release during ozone-induced asthma exacerbation and suppressed neutrophil lung infiltration. RTA-408 decreased pro-inflammatory cytokine production and reduced the percentage of IL-17+ γδT cells. Thus, our results suggest that RTA-408 does attenuate airway inflammation in a murine model of ozone-induced asthma exacerbation.

4.
Mol Med Rep ; 19(6): 4613-4622, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30957180

RESUMO

Gastric cancer (GC) is a leading cause of cancer­associated mortality worldwide. Previous studies demonstrated that long noncoding RNAs (lncRNAs) may be dysregulated in GC and may serve important roles in cancer progression. The present study aimed to investigate the role of the novel lncRNA stomach cancer­associated transcript 16 (STCAT16; Assembly Gene ID G038291) in the development and progression of GC. The present data suggested that the expression level of STCAT16 was decreased in GC tissues. The expression level of STCAT16 was identified to be associated with lymph node and tumour node metastasis stages. Furthermore, the expression level of STCAT16 was identified to be significantly associated with poor survival and prognosis. Knockdown of STCAT16 promoted proliferation, colony formation, migration and invasion of BGC­823 cells. In contrast, these features were suppressed in AGS cells following overexpression of STCAT16. In vivo, tumour growth was significantly decreased following STCAT16 overexpression. Collectively, the present data suggested that the lncRNA STCAT16 may act as a tumour suppressor and may inhibit GC tumour cell growth and migration. Additionally, the decreased expression level of STCAT16 was identified to be associated with poor prognosis in patients with GC.


Assuntos
Proliferação de Células , RNA Longo não Codificante/genética , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Linhagem Celular Tumoral , Transformação Celular Neoplásica/genética , Progressão da Doença , Regulação para Baixo , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , RNA Longo não Codificante/metabolismo , Neoplasias Gástricas/genética
5.
Orthop Surg ; 11(2): 294-303, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30989820

RESUMO

OBJECTIVE: Relatively few studies have reported on the morphology of the supracondylar femur, which is a fundamental factor affecting prosthetic reconstruction. The objectives of the present study were to measure the morphological parameters of the supracondylar femur, to classify the supracondylar femur, and to provide theoretical guidance for the development of distal femoral prostheses. METHODS: The study consisted of 82 patients of Han Chinese nationality in North China. There were 57 men and 25 women included in the study, with an average age of 50.9 years (range, 18-87 years). Effective CT data should include a range of more than 10 cm for the distal femur. CT data for the right distal supracondylar femurs was obtained from DICOM files. Results for the cancellous bone and marrow cavity were retained, and information for the cortical bone was erased to obtain information of the lumen. Measurements of the intracortical cavity have not been reported previously. Lumen models were reconstructed with Mimics 17.0 software. The surfaces of the lumen models were smoothed with Geomagic studio 12.0 software. Using the Solidworks 2014 software, we established a 3-D coordinate system, where variables of the lumen were examined. Correlations between the various measurements were calculated. RESULTS: The supracondylar region of the femur was divided into five levels, and the length, breadth, height, and angle values were measured at each level. There were strong correlations between the length indexes (transverse diameter [EF], medial anteroposterior diameter [AC], middle anteroposterior diameter [GH], and lateral anteroposterior diameter [BD]) and the volume index (V). There were also strong correlations among the length indicators (EF, AC, GH, and BD) in each layer. Angle γ was correlated with the lateral anteroposterior diameter (BD) at L2-L6 layers (r = -0.383, -0.385, -0.296, -0.258, -0.24; all P < 0.05) and with the height index (h) at L4-L6 layers (r = -0.244, -0.385, -0.506; all P < 0.05). The most representative parameters were the medial anteroposterior diameter (AC2 R2 = 0.865; AC6 R2 = 0.932), the coronal width ratio, and the sagittal width ratio with volume. The analysis found that the lumen shape of flower-top hat accounted for 81% at most. CONCLUSIONS: The supracondylar femur has an asymmetrical structural area. The coronal plane is dominated by a flowerpot-like morphology, and the sagittal plane is narrowest in the lateral 1/3 and resembles a top-hat-like morphology. Our results provide theoretical guidance for developing distal femoral prostheses and for their clinical application.

6.
J Am Chem Soc ; 141(13): 5326-5333, 2019 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-30856325

RESUMO

We report structural properties, physical properties, and the electronic structure of van der Waals (vdW) crystal VI3. Detailed analysis reveals that VI3 exhibits a structural transition from monoclinic C2/ m to rhombohedral R3̅ at Ts ≈ 79 K, similar to CrX3 (X = Cl, Br, I). Below Ts, a long-range ferromagnetic (FM) transition emerges at Tc ≈ 50 K. The local moment of V in VI3 is close to the high-spin state V3+ ion ( S = 1). Theoretical calculations suggest that VI3 may be a Mott insulator with a band gap of about 0.90 eV. In addition, VI3 has a relatively small interlayer binding energy and can be exfoliated easily down to a few layers experimentally. Therefore, VI3 is a candidate for two-dimensional FM semiconductors. It also provides a novel platform to explore 2D magnetism and vdW heterostructures in S = 1 system.

7.
Gene ; 698: 34-40, 2019 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-30802538

RESUMO

MicroRNA-499 (miR-499) rs3746444 polymorphism has been associated with the risk of coronary heart disease (CHD). However, results from several studies are inconsistent. This meta-analysis aimed to further investigate the possible association between miR-499 rs3746444 polymorphism and CHD risk. A total of 9 case-control studies included 5063 CHD cases and 4603 healthy subjects. The A allele at rs374644 was associated with significantly decreased CHD risk in the total population according to the allelic model (OR = 0.80, 95% CI = 0.68-0.93, P = 0.005), homozygous model (OR = 0.52, 95% CI = 0.39-0.71, P < 0.001) and heterozygous model (OR = 0.57, 95% CI = 0.43-0.77, P < 0.001). A similar trend was found specifically in Asian and Chinese populations. In contrast, the wild-type GG genotype at rs374644 was associated with significantly increased CHD risk in the total population, according to the dominant model (OR = 1.83, 95% CI = 1.39-2.42, P < 0.001), and a similar trend was found in Asian and Chinese populations. These results indicate that in the total population, as well as in Asian and Chinese populations, the wild-type GG genotype at rs374644 may be related to increased susceptibility to CHD, while the A allele may be protective against CHD.


Assuntos
Doença das Coronárias/genética , MicroRNAs/genética , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China , Frequência do Gene/genética , Predisposição Genética para Doença/genética , Genótipo , Humanos , MicroRNAs/metabolismo , Polimorfismo de Nucleotídeo Único/genética , Fatores de Risco
8.
J Plast Reconstr Aesthet Surg ; 72(4): 628-635, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30655243

RESUMO

We described a treatment of fingertip avulsion injury by two periposition pedicled flaps, the reversed pedicle island flap of dorsal branch of proper digital artery and the cross-finger flap. From February 2009 to January 2017, 22 patients (22 fingers) with fingertip avulsion injury were treated with two periposition pedicled flaps, 14 male and 8 female, aged from 17 to 47 years (mean 32 ± 9 years). All of the patients were followed up more than 6 months after operations (mean 9.6 ± 2.6 months). All flaps survived completely. Compared with the traditional abdominal flaps, two periposition pedicled flaps had less pedicle division time. At last follow-up, the 2PD of the palmar part of the flaps, the TAM of the injured finger and the MHQ summary score of the two periposition pedicled flaps were much better than the traditional abdominal flaps. The reconstruction using two periposition pedicled flaps is a versatile treatment with better functions, less morbidity and better aesthetics. Level of evidence: Therapeutic, level III.

9.
Huan Jing Ke Xue ; 40(2): 701-707, 2019 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-30628333

RESUMO

Chromium (Cr) contamination caused by industrial manufacturing poses a severe challenge in the environment. Titanium dioxide (TiO2) has potential application in Cr removal due to its adsorption and photocatalytic performance. High-index TiO2 with exposed {201} facet was synthesized using the solvothermal method and characterized by SEM, TEM, XRD, and XPS. The adsorption of Cr(Ⅲ/Ⅵ) and photocatalytic reduction of Cr(Ⅵ) on TiO2{201} was examined for the removal from water. The synthesized TiO2{201} was constructed by a dandelion-like hierarchical structure. The adsorption isotherms of Cr(Ⅲ) and Cr(Ⅵ) on TiO2{201} conformed to the Langmuir model, with maximum adsorption capacities of 22.7 mg·g-1 and 13.2 mg·g-1, respectively. The best fitted results from the Freundlich model show that the adsorption of Cr(Ⅲ) and Cr(Ⅵ) on TiO2{201} were favorable with the parameter of 1/n less than 0.5. The results of photocatalytic reduction show that TiO2{201} can reduce Cr(Ⅵ) to Cr(Ⅲ) under UV irradiation, and Cr(Ⅲ) was further precipitated on the surface of TiO2 in the form of Cr(OH)3 and Cr2O3, which was evidenced by XPS characterization. To explore the mechanism of photocatalytic reduction of Cr(Ⅵ), the effect of scavengers for photogenerated holes (EDTA-2Na) and electrons (KBrO3) on Cr(Ⅵ) reduction was studied, and the results suggested that photogenerated electrons were the main reductant.

10.
Orthop Surg ; 10(4): 306-311, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30474324

RESUMO

OBJECTIVE: Turnover of cartilage endplate extracellular matrix (ECM) may play an important role in disc degeneration and low back pain (LBP). However, the expression pattern of pro-inflammatory factors, matrix metalloproteinases (MMP), and tissue inhibitors of metalloproteinases (TIMP) in the cartilage endplates (CEP) of intervertebral discs (IVD) is not understood. We aimed to examine the transcriptional levels of MMP, TIMP, and interleukins (IL), and the correlations between them. METHODS: Thirty degenerated cartilage endplate samples from patients with LBP who underwent lumbar fusion surgery were included in the degenerated group. Ten patients without LBP history who underwent lumbar surgery because of vertebral burst fractures were included in the control group. The degenerative severity of the samples was evaluated by MRI, and hematoxylin-eosin and safranin O-fast green (SO-FG) staining. Real-time polymerase chain reaction (RT-PCR) was used to detect the mRNA levels of MMP-1, MMP-3, MMP-9, MMP-13, TIMP-1, TIMP-2, TIMP-3, IL-1α, IL-1ß, and IL-6. The correlations between the levels of these genes were tested using Spearman's rho test. RESULTS: Hematoxylin-eosin and SO-FG staining confirmed a decrease in cell number and proteoglycans in the degenerated cartilage endplate. MRI showed significant signal changes in degenerated cartilage endplates. Patients in the degenerated group showed a higher rate of endplate Modic changes when compared with the control group. MMP-3, MMP-9, TIMP-3, IL-1α, and IL-1ß were elevated with statistical significance, while MMP-1, MMP-13, TIMP-1, TIMP-2, and IL-6 were changed without statistical significance or remained unchanged. Expression of MMP-3 was positively correlated with IL-1α (Spearman coefficient, 0.486; P < 0.05); expression of TIMP-3 was positively correlated with MMP-9, IL-1α, and IL-1ß (Spearman coefficient, 0.577, 0.407, and 0.571, respectively; P < 0.05). CONCLUSION: MMP-3, MMP-9, TIMP-3, IL-1α, and IL-1ß may play a role in the process of cartilage endplate degeneration. MMP-3 may be regulated by IL-1α, and TIMP-3 might be associated with MMP-9 and regulated by IL-1α and IL-1ß.

11.
J Cell Physiol ; 2018 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-30480800

RESUMO

Patent ductus arteriosus is the third most common congenital heart disease and resulted from the persistence of ductal patency after birth. Ductus arteriosus closure involves functional and structural remodeling, controlled by many factors. The changes in plasma protein levels associated with PDA closure are not known. Here we for the first time demonstrate six key differential plasma proteins in human patent ductus arteriosus patients using proteomic technology and present a model to illustrate the constriction and closure of ductus arteriosus. Differentially expressed proteins were analyzed by using isobaric tags for relative and absolute quantification and validated by enzyme-linked immunosorbent assay in new samples. The proteomic data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the data set identifier PXD008568. We found 74 upregulated and 98 downregulated proteins in the plasma of patients with PDA. Five decreased proteins (platelet factor 4, fibrinogen, von Willebrand factor, collagen, and mannose binding lectin-associated serine protease-2) and one increased protein (fibronectin) may increase the risk of patent ductus arteriosus. Those proteins are closely related to platelet activation and coagulation cascades, complement mannan-binding-lectin, and other systemic signaling pathways. Our findings for the first time indicate that the differential proteins involved in different pathways may play key roles in the nonclosure of the ductus arteriosus in humans and may be developed as biomarkers for diagnosis. All those findings may be served as the basis of understanding the etiology and pathogenesis of patent ductus arteriosus.

12.
Ying Yong Sheng Tai Xue Bao ; 29(10): 3416-3424, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30325168

RESUMO

Phosphorus is the necessary element for plant growth, and its concentration is one of the main indices for water eutrophication. Hence, it is significant to understand how woody plants purify phosphorus in eutrophic water. The purpose of this study is to reveal the P absorption and removal mechanism of Salix matsudana in eutrophic water with different P concentration. We selected new S. matsudana clone (A42) as experimental material and set three levels of P concentration (low P: 0.1, 0.2 mg·L-1; medium P: 1.0, 2.0 mg·L-1, high P: 10.0 mg·L-1), and the floating bed hydroponic experiment was conducted at the greenhouse from July to September, 2017. We found that S. matsudana efficiently removed P in water (removal rate >79% in 21 days). There was a positive correlation between the removal quantity and P concentration in the water. The removal ratio rose at first and then fell with increasing P. Owing to the purification of S. matsudana, the P concentrations ranging from 0.1 mg·L-1 to 1.0 mg·L-1 were reduced to minimum threshold concentration of eutrophication (0.016-0.032 mg·L-1) in seven days. The percentage of phosphorus input in water that assimilated by S. matsudana ranged from 29.0% to 66.9%. The quantity and ratio of assimilated P were respectively positive and negative relation with P concentration. Salix matsudana adapted to eutrophic water with different P concentrations and normally grew during experiment period, with root-shoot ratio being significantly increased with decreases of water P concentrations. The characteristic of phosphorus distribution in plant organs was: stem> leave>root, while the translocation factors (TF) of nitrogen and phosphorus were both greater than 3. When S. matsudana grew in eutrophic water with high phosphorus concentration, the TF of nitrogen and phosphorus significantly increased to 4.53±0.24 and 4.92±0.62 respectively. Our results indicated that S. matsudanais could purify the eutrophic water and it could normally grow. New clone of S. matsudana could effectively absorb phosphorus in the water and accumulated it in the stem, which could reduce secondary pollution. In conclusion, S. matsudana could be used for a short-term treatment on the eutrophic water with low P concentration, while for the long-term treatment it is adapted to eutrophic water with high phosphorus concentrations.


Assuntos
Salix , Biodegradação Ambiental , Eutrofização , Hidroponia , Nitrogênio , Fósforo , Desenvolvimento Vegetal , Água
13.
Aging Dis ; 9(5): 785-797, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30271656

RESUMO

Aging is an inevitable physiological challenge occurring in organisms over time, and is also the most important risk factor of neurodegenerative diseases. In this study, we observed cellular and molecular changes of different age mice and LPS-induced Parkinson disease (PD) model. The results showed that behavioral performance and dopaminergic (DA) neurons were declined, accompanied by increased expression of pro-inflammatory factors (TLR2, p-NF-kB-p65, IL-1ß and TNF-α), as well as pro-oxidative stress factor gp91phox in aged mice compared with young mice. Aging exaggerated inflammatory M1 microglia, and destroyed the balance between oxidation and anti-oxidation. The intranasal LPS instillation induced PD model in both young and aged mice. The poor behavioral performance and the loss of DA neurons as well as TLR2, p-NF-kB-p65, IL-1ß, TNF-α, iNOS and gp91phox were further aggravated in LPS-aged mice. Interestingly, the expression of Nrf2 and HO-1 was up-regulated by LPS only in young LPS-PD mice, but not in aged mice. The results indicate that the synergy of aging process and LPS exposure may prominently aggravate the DA neurons loss caused by more serious neuroinflammation and oxidative stress in the brain.

14.
Life Sci ; 210: 1-8, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-30121199

RESUMO

AIMS: Although therapeutic strategies for acute respiratory distress syndrome (ARDS) have achieved improvements, its mortality remains high. It has been reported that microRNAs (miRs) serve as therapeutic strategies for ARDS, while specific mechanisms of miR-494 remain poorly understood. Thus, the present study aimed to assess the effects of miR-494 on acute lung injury (ALI) in rat models of sepsis-associated ARDS and its regulatory mechanism. METHODS: Following establishment of sepsis-associated ARDS rat models, the ratio of wet to dry weight (W/D) in right lung tissues was detected. Moreover, the expression patterns of miR-494, NQO1 and Nrf2 were evaluated in left lung tissues of rats. The miR-494 was exogenously overexpressed in rats so as to analyze the effects of miR-494 on ALI, inflammatory response and oxidative stress. Meanwhile, the Nrf2 signaling pathway was activated in rats in order to show the regulatory mechanism of miR-494 in ALI. And the target gene of miR-494 was identified by dual-luciferase reporter assay. KEY FINDINGS: The findings firstly revealed upregulated miR-494, and enhanced inflammatory response, oxidative stress and ALI in rat models of sepsis-associated ARDS. Additionally, MiR-494 negatively regulated NQO1 and blocked the Nrf2 signaling pathway. Moreover, ectopic expression of miR-494 promoted inflammatory response, oxidative stress and ALI. However, the activation of Nrf2 signaling pathway reversed these effects of miR-494. SIGNIFICANCE: Our key findings highlight the value of miR-494 inhibition as a therapeutic target for sepsis-associated ARDS, as a result of miR-494 accelerated ALI in rats with sepsis-associated ARDS through NQO1-mediated inactivation of Nrf2 signaling pathway.


Assuntos
Lesão Pulmonar Aguda/prevenção & controle , Modelos Animais de Doenças , MicroRNAs/genética , NAD(P)H Desidrogenase (Quinona)/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Síndrome do Desconforto Respiratório do Adulto/patologia , Sepse/complicações , Lesão Pulmonar Aguda/etiologia , Lesão Pulmonar Aguda/genética , Animais , Masculino , NAD(P)H Desidrogenase (Quinona)/genética , Fator 2 Relacionado a NF-E2/genética , Estresse Oxidativo , Ratos , Ratos Sprague-Dawley , Síndrome do Desconforto Respiratório do Adulto/etiologia , Síndrome do Desconforto Respiratório do Adulto/metabolismo , Transdução de Sinais , Regulação para Cima
15.
Orthop Surg ; 10(3): 281-284, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30073780

RESUMO

Diaphyseal defects in long bones of extremities following segmental resection are very common in the clinic, and reconstruction remains a great challenge. Although there are many treatments for diaphyseal defects at present, reconstruction with an intercalary endoprosthesis may be an optimal method. We demonstrate a surgical technique for reconstruction of a humeral shaft defect with an intercalary endoprosthesis following tumor resection, and achieve a good clinical outcome. We conclude that in comparison with other methods, reconstruction with an intercalary endoprosthesis is simple, effective, and allows for earlier weight bearing and more rapid restoration of function.

16.
Ying Yong Sheng Tai Xue Bao ; 29(6): 1960-1968, 2018 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-29974706

RESUMO

A field experiment was conducted with sandy fluvo-aquic soil under wheat-maize rotation system during 2012 to 2016 in Henan Province, with four treatments: Chemical fertilizer (CK), composted sewage sludge (CS) 15 t·hm-2(CS1), 30 t·hm-2(CS2), 45 t·hm-2(CS3). We investigated the effects of CS on soil active organic nitrogen and its allocation ratio. Results showed that CS significantly enhanced soil water content, decreased soil pH, increased contents of soil organic carbon and total nitrogen (TN) and available nutrients. Soil TN was increased by 93.1%-284.3%. The CS3 treatment significantly improved the content of light fraction organic nitrogen (LFON), particulate organic nitrogen (PON) and microbial biomass nitrogen (MBN). CS treatments promoted the allocation of PON/TN and MBN/TN. with an enhancement of 12.3% and 539.9% in the CS2 treatment, respectively. The distributions of LFON/TN and DON/TN were decreased by 17.3%-40.1% and 38.5%-71.3%, respectively. The contribution of soil active organic nitrogen fractions to TN was: PON>LFON>DON>MBN. Results from the principal component analysis showed that organic nitrogen and their distribution had high load value in CS2 and CS3 treatments. Results from redundancy analysis showed that positive correlations existed between the soil physicochemistry, enzymatic activity and soil active organic nitrogen as well as their distribution. In summary, CS had positive impacts on soil organic matter, soil properties, and the formation of active organic nitrogen. The rates of 30 and 45 t·hm-2 CS application had significant effects on soil fertility, which could be an effective way to improve sandy fluvo-aquic soil quality.


Assuntos
Compostagem , Nitrogênio/análise , Esgotos , Carbono , Fertilizantes , Solo
17.
Infect Dis Poverty ; 7(1): 62, 2018 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-29961423

RESUMO

BACKGROUND: The prevalence of schistosomiasis japonica has decreased significantly, and the responses changing from control to elimination in Jiangsu Province, P.R. China. How to estimate the change in prevalence of schistosomiasis using only serological data will be important and useful. METHODS: We collected serum samples from 2011 to 2015 to build a serum bank from Dantu County of Jiangsu, China. Serum samples were detected by enzyme-linked immunosorbent assay (ELISA), the positive rate and optical density (OD) value were obtained. The Bayesian model including the prior information of sensitivity and specificity of ELISA was established, and the estimated infection rates were obtained for different years, genders and age groups. RESULTS: There was no significant difference in the mean OD between different years and genders, but there was a significant difference between the different age groups. There were statistically significant differences in the positive rate for different years and age groups, but no significant difference at different genders. The estimated infection rate for the five years was 1.288, 1.456, 1.032, 1.485 and 1.358%, respectively. There was no significant difference between different years and between genders, but a significant difference between different age groups. CONCLUSIONS: The risk of schistosomiasis transmission in this area still exists, and risk monitoring of schistosomiasis should be strengthened.


Assuntos
Schistosoma japonicum/isolamento & purificação , Esquistossomose Japônica/epidemiologia , Adolescente , Adulto , Idoso , Animais , Teorema de Bayes , Criança , China/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Esquistossomose Japônica/parasitologia , Sensibilidade e Especificidade , Estudos Soroepidemiológicos , Adulto Jovem
18.
Huan Jing Ke Xue ; 39(3): 1241-1247, 2018 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965469

RESUMO

Titanium dioxide (TiO2) was widely used to remove arsenic (As) from groundwater due to its excellent properties. Previous studies show that the coexisting silicate ions (Si) could occupy the available surface sites of TiO2 and further inhibit As adsorption and TiO2 regeneration. To investigate the effect of Si adsorption on the As molecular surface structure, an extended X-ray absorption fine structure (EXAFS) analysis was conducted in this work. The results indicated that the presence of Si exhibited no impact on the As adsorption configuration on TiO2. In situ attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy results demonstrated that the polymerization of Si that formed on the TiO2 surface compete with As adsorption sites, increasing the difficulty for TiO2 regeneration. To effectively regenerate TiO2, the removal efficiency of Si polymers on TiO2 via sodium fluoride (NaF) was studied. The results showed that NaF could remove Si monomer and polymer from TiO2, and the regenerated TiO2 could be reused with a stable adsorption performance. In situ ATR-FTIR spectroscopy suggested that NaF desorbed the Si monomer and polymer effectively. When spent TiO2 was regenerated with NaOH and NaF in three treatment cycles, As and Si desorption rates were 86.8%-100.3% and 67.9%-82.0%, respectively. The present study provides a new insight into regenerating absorbents with coadsorbed As and Si in groundwater.

19.
Chin J Integr Med ; 2018 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-29790062

RESUMO

OBJECTIVE: To collect and analyze multi-dimensional pulse diagram features with the array sensor of a pressure profile system (PPS) and study the characteristic parameters of the new multi-dimensional pulse diagram by pulse diagram analysis technology. METHODS: The pulse signals at the Guan position of left wrist were acquired from 105 volunteers at the Shanghai University of Traditional Chinese Medicine. We obtained the pulse data using an array sensor with 3×4 channels. Three dimensional pulse diagrams were constructed for the validated pulse data, and the array pulse volume (APV) parameter was computed by a linear interpolation algorithm. The APV differences among normal pulse (NP), wiry pulse (WP) and slippery pulse (SP) were analyzed using one-way analysis of variance. The coefficients of variation (CV) were calculated for WP, SP and NP. RESULTS: The APV difference between WP and NP in the 105 volunteers was statistically significant (6.26±0.28 vs. 6.04±0.36, P=0.048), as well as the difference between WP and SP (6.26±0.28 vs. 6.07±0.46, P=0.049). However, no statistically significant difference was found between NP and SP (P=0.75). WP showed a similar CV (4.47%) to those of NP (5.96%) and SP (7.58%). CONCLUSION: The new parameter APV could differentiate between NP or SP and WP. Accordingly, APV could be considered an useful parameter for the analysis of array pulse diagrams in Chinese medicine.

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