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1.
Technol Cancer Res Treat ; 18: 1533033819883633, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31684829

RESUMO

OBJECTIVE: To investigate the effect of long noncoding RNA GM16343 on interleukin 36ß promotion of CD8+T cells in tumor microenvironment regulation. METHODS: The differentially expressed long noncoding RNA in interleukin 36ß-stimulated mouse CD8+T cells was screened by gene chip technology, and the significant differentially expressed long noncoding RNAs were verified by real-time polymerase chain reaction. The lentiviral vector that overexpresses or knockdown GM16343 was constructed, transfected into CD8+T cells, and stimulated with interleukin 36ß, and the amount of interferon γ secreted was detected by enzyme-linked immunosorbent assay. A mouse subcutaneous xenograft model that stably express interleukin 36ß was established, and the tumor size and mouse survival time were observed by stimulation with CD8+T cells overexpression or knockdown of GM16343. RESULTS: A total of 12 long noncoding RNAs with significant differences were screened by gene chip analysis. Real-time polymerase chain reaction showed that the difference in GM16343 was larger, and the difference between the groups was observed to be the most significant. Compared to control group, CD8+T cells overexpressing GM16343 increased the secretion of interferon γ, and the tumor diameter of the mice after stimulation showed significant reduction, and the survival time showed significant prolongation. Compared to control group, the CD8+T cells after GM16343 were knocked down. The interferon γ secretion was decreased, and no significant change in tumor diameter and survival time was observed. CONCLUSION: Interleukin 36ß may enhance antitumor immune response of CD8+T cells by regulating GM16343.

2.
Int Urol Nephrol ; 50(11): 1981-1988, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30242548

RESUMO

PURPOSE: To explore the neuroprotective effects and its possible mechanisms of melatonin (MT) on erectile dysfunction in streptozotocin-induced diabetic rats. METHODS: Twenty-eight Sprague-Dawley rats received intraperitoneal injection of streptozotocin and 8 weeks later, the determined diabetic rats randomly got intraperitoneal injection of phosphate buffer solution (PBS) or MT. Another 12 normal rats received PBS treatment. Four weeks later, intracavernous pressure, mean arterial pressure, pathological changes in penis, and major pelvic ganglion (MPG) were measured. Malondialdehyde, superoxide dismutase, p38 and p-p38 levels in penis were detected. RESULTS: Diabetic rats showed significant decreases of erectile function accompanied with serious neuropathy in dorsal penile nerve (DPN) and MPG, meanwhile collagen deposition, oxidative stress, and p-p38 levels in penis were elevated. Melatonin treatment partially but significantly improved the erectile function, ameliorated neuropathy in DPN and MPG, and decreased collagen deposition, oxidative stress, and p-p38 levels in diabetic rats. CONCLUSIONS: Melatonin treatment helps improve erectile function and ameliorate neuropathy and fibrosis in diabetic rats. These may be associated with reductions in oxidative stress, p38MAPK signaling pathway, and neuropathy.


Assuntos
Antioxidantes/uso terapêutico , Diabetes Mellitus Experimental/complicações , Disfunção Erétil/tratamento farmacológico , Melatonina/uso terapêutico , Animais , Disfunção Erétil/etiologia , Masculino , Estresse Oxidativo , Ratos , Ratos Sprague-Dawley , Estreptozocina
3.
Langmuir ; 33(29): 7248-7255, 2017 07 25.
Artigo em Inglês | MEDLINE | ID: mdl-28681601

RESUMO

In this paper, we report the preparation of anisotropic wetting surfaces that could control various wetting behaviors of liquids in a wide surface tension range (from water to oil), which could be employed as a platform for controlling the flow of liquids in microfluidics (MFs). The anisotropic wetting surfaces are chemistry-asymmetric "Janus" silicon cylinder arrays, which are fabricated via selecting and regulating the functional groups on the surface of each cylinder unit. Liquids (in a wide surface tension range) wet in a unidirectional manner along the direction that was modified by the group with large surface energy. Through introducing the Janus structure into a T-shaped pattern and integrating it with an identical T-shaped poly(dimethylsiloxane) microchannel, the as-prepared chips can be utilized to perform as a surface tension admeasuring apparatus or a one-way valve for liquids in a wide surface tension range, even oil. Furthermore, because of the excellent ability in controlling the flowing behavior of liquids in a wide surface tension range in an open system or a microchannel, the anisotropic wetting surfaces are potential candidates to be applied both in open MFs and conventional MFs, which would broaden the application fields of MFs.

4.
Chronic Dis Transl Med ; 2(4): 250-256, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29063050

RESUMO

OBJECTIVE: To retrospectively evaluate appropriate treatment for patients with symptomatic caliceal diverticular calculi, by comparing the therapeutic outcomes for those undergoing minimally invasive percutaneous nephrolithotomy (MPCNL) and flexible ureterorenoscopy (F-URS). METHODS: From March 2009 to May 2014, 36 consecutive patients with caliceal diverticular calculi were divided into 2 groups: 21 patients underwent MPCNL, and 15 were treated by F-URS. All procedures were performed by one surgical group, which ensured relatively constant parameters. Patient characteristics, operative time, hospital stay after surgery, stone-free rate, symptomatic improvement rate, complications, diverticular obliteration, and stone composition were analyzed retrospectively in the 2 groups. RESULTS: Patient preoperative variables were comparable between the two groups, with no significant difference (P > 0.05). Mean operative time was 136.9 ± 22.8 min in the MPCNL group and 117.3 ± 24.3 min in the F-URS group (P = 0.019). Hospital stay was significantly longer in the MPCNL group than in the F-URS group (9.4 ± 3.1 vs. 6.9 ± 2.1 days, P = 0.010). The stone-free rates after MPCNL and F-URS were 90.5% (19/21) and 60.0% (9/15), respectively (P = 0.046). Additionally, 71.4% (15/21) of patients in the MPCNL group and 46.7% (7/15) of patients in the F-URS group had symptomatic improvement at the 6-month follow-up (P = 0.175); the rates of complications in the 2 groups were 19.0% (4/21) and 13.3% (2/15), respectively (P = 0.650). Complete diverticular obliteration was achieved in 16 (76.2%) cases in the MPCNL group and 5 (33.3%) cases in the F-URS group (P = 0.017). The distributions of calcium oxalate and hydroxyapatite in the stones were 66.7% (14/21) and 33.3% (7/21), respectively, in the MPCNL group; however, the distributions in the F-URS group were 46.7% (7/15) and 53.3% (8/15), respectively (P = 0.310). CONCLUSION: MPCNL is an effective method for the treatment of caliceal diverticular calculi. However, F-URS is an alternative technique in selected patients with a patent infundibulum, despite lower stone-free rates than with MPCNL. Fulguration of the diverticular lining with a high-power holmium laser and permitting the cavity to collapse are useful to increase the chance of diverticular obliteration.

5.
Chin Med J (Engl) ; 126(15): 2815-20, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23924448

RESUMO

BACKGROUND: Humoral immunity is an important factor for long-term survival of renal allograft. Here we performed a four-year follow-up to explore the clinical significance of monitoring anti-human leukocyte antigens (HLA) and anti-major histocompatibility complex class I-related chain A (MICA) antibody expression after kidney transplantation. METHODS: We obtained serial serum samples from 84 kidney transplant patients over a four-year period. All patients were followed up at least 6 months after transplantation and had at least two follow-up points. Anti-HLA and anti-MICA antibody titres and serum creatinine (SCr) levels were evaluated at each follow-up. Patients were divided into 4 groups: HLA(+) MICA(-), HLA(-)MICA(+), HLA(+)MICA(+) and HLA(-)MICA(-). The impact of post-transplant antibody level on kidney allograft function was evaluated. RESULTS: Antibodies were detected in 38.1% (32/84) of the renal allograft recipients. HLA, MICA and HLA+MICA expression was observed in 18.89%, 14.44% and 5.93% of the recipients respectively. The most frequent anti-HLA and anti-MICA specific antibodies identified were A11, A24, A29, A32, A33, A80; B7, B13, B37; DR17, DR12, DR18, DR52, DR53, DR1, DR4, DR9, DR51; DQ7, DQ4, DQ8, DQ2, DQ9, DQ5, DQ6 and MICA02, MICA18, MICA19, MICA07, MICA27. As the time after transplantation elapsed, more recipients developed de novo antibody expression. Total 11.91% (10/84) of the recipients had de novo antibody expression during the follow up. The average level of SCr and the percentage of recipients with abnormal allograft function were significantly higher in recipients with anti-HLA and/or anti-MICA antibody expression than those without. The appearance of anti-HLA and anti-MICA antibody expression always preceded the increase in SCr value. CONCLUSIONS: Anti-HLA and anti-MICA antibody expression has predictive value for early and late allograft dysfunction. The presence of donor specific antibody is detrimental to graft function and graft survival.


Assuntos
Antígenos HLA/imunologia , Antígenos de Histocompatibilidade Classe I/imunologia , Transplante de Rim , Feminino , Seguimentos , Sobrevivência de Enxerto , Humanos , Isoanticorpos/análise , Masculino , Antígenos de Histocompatibilidade Menor
6.
Urology ; 82(4): 974.e9-13, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23947989

RESUMO

OBJECTIVE: To examine the frequency of tumor-infiltrating Tie-2-expressing monocytes (TEMs) in renal cell carcinoma (RCC) and its association with microvessel density (MVD) and other clinical-pathologic features. MATERIALS AND METHODS: This study enrolled 65 consecutive patients with RCC treated with radical nephrectomy. The frequency of tumor-infiltrating TEMs, which was defined as CD14(+) Tie-2(+) cells, was assessed using flow cytometry. MVD was measured by immunohistochemistry using anti-CD34 antibody. The association between clinicopathologic parameters, MVD, and the frequency of tumor-infiltrating TEMs in RCC was assessed. RESULTS: High frequency of tumor-infiltrating TEMs was significantly associated with advanced stage (P = .018), positive lymph nodes (P = .013), high grade (P = .019), and metastases (P = .006). Correlation analysis revealed that the frequency of TEMs was positively correlated with MVD. CONCLUSION: Our findings revealed a significant association between prognostic tumor features, MVD, and the frequency of tumor-infiltrating TEMs in RCC and indicated that TEMs may play an important role in angiogenesis and progression of RCC.


Assuntos
Carcinoma de Células Renais/metabolismo , Neoplasias Renais/metabolismo , Monócitos/metabolismo , Receptor TIE-2/biossíntese , Adulto , Idoso , Carcinoma de Células Renais/patologia , Progressão da Doença , Feminino , Humanos , Neoplasias Renais/patologia , Masculino , Pessoa de Meia-Idade , Neovascularização Patológica
7.
Oncol Rep ; 29(3): 1019-26, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23254994

RESUMO

Angiogenesis is known to be essential to the survival, growth, invasion and metastasis of cancer cells. Vascular endothelial growth factor (VEGF) is an important factor regulating tumor angiogenesis. In the present study, we analyzed the effect of lentivirus-mediated shRNA interference targeting vascular endothelial growth factor (VEGF) on angiogenesis and progression in the pancreatic cancer cell line Patu8988 in vitro and in vivo. The study aimed to construct a recombinant lentivirus carrying targeted VEGF shRNA (LV-RNAi) to be used to transfect Patu8988 cells, and we investigated its anti-angiogenic and growth inhibitory effects on pancreatic cancer. VEGF expression was measured by RQ-PCR, western blotting and enzyme-linked immunosorbent assay (ELISA). In subcutaneous transplantation models, tumor volumes were determined, and the expression levels of VEGF and CD34 were assessed by immunohistochemistry. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was used to determine apoptosis. In the orthotopic transplantation models, tumor volume and liver metastasis were determined. We successfully constructed LV-RNAi and confirmed that it knocked down the VEGF gene at the mRNA and protein levels in Patu8988 cells. In the subcutaneous transplantation models, tumors with low levels of VEGF expression exhibited reduced pancreatic carcinoma angiogenesis and growth, and the apoptotic index was significantly higher. In the orthotopic transplantation models, tumors with low levels of VEGF expression exhibited significantly reduced pancreatic carcinoma growth, but no significant difference was observed between the three mouse groups, LV-RNAi, LV-NC and the control, in regards to liver metastasis. In summary, lentivirus-mediated RNAi silencing of VEGF inhibited tumor angiogenesis and growth, and increased apoptosis of the pancreatic cancer cell line Patu8988. VEGF targeted gene silencing approach has the potential to serve as a novel treatment for pancreatic cancer.


Assuntos
Adenocarcinoma/terapia , Terapia Genética , Neoplasias Hepáticas/terapia , Neovascularização Patológica/prevenção & controle , Neoplasias Pancreáticas/terapia , RNA Interferente Pequeno/genética , Fator A de Crescimento do Endotélio Vascular/genética , Adenocarcinoma/irrigação sanguínea , Adenocarcinoma/genética , Adenocarcinoma/secundário , Animais , Antígenos CD34/metabolismo , Apoptose , Sequência de Bases , Linhagem Celular Tumoral , Progressão da Doença , Técnicas de Silenciamento de Genes , Humanos , Lentivirus/genética , Neoplasias Hepáticas/irrigação sanguínea , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/secundário , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Dados de Sequência Molecular , Transplante de Neoplasias , Neovascularização Patológica/genética , Neoplasias Pancreáticas/irrigação sanguínea , Neoplasias Pancreáticas/genética , Neoplasias Pancreáticas/patologia , Interferência de RNA , Análise de Sequência de DNA , Transfecção , Carga Tumoral , Fator A de Crescimento do Endotélio Vascular/metabolismo
8.
Asian Pac J Cancer Prev ; 13(8): 3931-6, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23098495

RESUMO

BACKGROUND: Epidemiological studies evaluating the association of two variants rs9340799 and rs2234693 on estrogen receptor 1 (ESR1) with prostate risk have generated inconsistent results. METHODS: A meta-analysis was here conducted to systematically evaluate the relationship of these two variants with prostate cancer susceptibility. RESULTS: For rs9340799, heterozygosity of T/C carriers showed a significant increased prostate cancer risk with a pooled odds ratio (OR) of 1.34 (95% CI = 1.06-1.69) while homozygote C/C carriers showed an increased but not statistically significant association with prostate cancer risk (pooled OR = 1.29, 95% CI = 0.94-1.79). Compared to the homozygous TT carriers, the allele C carriers showed a 31% increased risk for prostate cancer (pooled OR = 1.31, 95% CI = 1.06-1.63). No significant association between the rs2234693 and prostate cancer risk was found with the pooled OR of 1.15 (95% CI = 0.97-1.39, T/C and C/C vs. T/T) under the dominant genetic model. Compared to the homozygote T/T carriers, the heterozygous T/C carriers did not show any significantly different risk of prostate cancer (pooled OR = 1.13, 95% CI = 0.94-1.36) and the homozygous C/C carriers also did not show a significant change for prostate cancer risk compared to the wide-type T/T carriers (pooled OR = 1.26, 95% CI = 0.98-1.62). CONCLUSIONS: These data suggested that variant rs9340799, but not rs2234693, on ESR1 confers an elevated risk of prostate cancer.


Assuntos
Receptor alfa de Estrogênio/genética , Predisposição Genética para Doença , Polimorfismo Genético/genética , Neoplasias da Próstata/etiologia , Estudos de Casos e Controles , Humanos , Masculino , Fatores de Risco
9.
Med Hypotheses ; 75(5): 422-4, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20457493

RESUMO

The traditional theories believe that vacuum constriction devices (VCD) is the mechanism through a suction chamber promoting penile blood engorgement and maintaining tumescence with a constriction band. For that mechanical nature of the device, many authors and patients consider the devices are passive engorgement, lack of spontaneous erections, and only rely on the constriction bands to prevent venous outflow to maintain the erection. However, recent clinical studies show that many patients using the VCD devices without the constriction band, but still maintain the erections and return to natural erections. The current theories cannot explain this phenomenon. We think that the VCD devices not only through the mechanical nature to prevent venous outflow by the constriction bands to maintain the erection, but also through the suction chamber to stretch and stimulate the corpora cavernosa nerves, muscles, and blood vessels, and led to the neurotransmitter nitric oxide (NO) released. NO as the principal messenger molecule though the NO/cGMP signaling pathway to mediate corporal smooth muscle relaxation and penile erection. The relaxation of corporal smooth muscle creates pressure in the tunica albuginea can prevent venous outflow and maintain erection. This can explains the phenomenon of many patients using the VCD devices without the constriction bands also maintain the erections and return to natural erections. Thus, we put forward the hypothesis that the classical NO/cGMP signaling pathway is also involved in the penile erections when using the VCD devices. In the future, we could design and apply rat-specific VCD devices without the constriction bands in rat model to analyze the cell and molecular changes in rat corpora cavernosa to test this hypothesis.


Assuntos
GMP Cíclico/metabolismo , Óxido Nítrico/metabolismo , Ereção Peniana , Transdução de Sinais , Humanos , Masculino , Vácuo
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