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1.
Infect Drug Resist ; 16: 87-104, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36636379

RESUMO

Objective: To analyze the clinical characteristics and treatment process of 14 patients with aneurysm due to brucellosis, summarize the morbidity characteristics, and improve the knowledge and diagnosis of the disease. Methods: The clinical data of patients with aneurysms who were diagnosed to have brucellosis through Rose Bengal Test (RBT), Serum Agglutination Test (SAT), blood culture, and computed tomography angiography from January 2012 to November 2022 in Bethune Hospital, Shanxi Province, China, were retrospectively analyzed, and their clinical characteristics and disease regression findings were summarized. Results: Of the 14 patients, 13 were men, and 1 was a woman; the youngest patient was 48-year-old, and the oldest patient was of 74 years. All had a history of smoking. The sites of lesion occurrence included the abdominal aorta (5 patients), abdominal aorta +iliac artery (5 patients), iliac artery (2 patients), thoracic aorta and brachial artery were less common (1 case each). Symptoms in the patients mostly began with abdominal pain, which was accompanied by fever, nausea, and vomiting. Six patients had a clear history of livestock exposure. Fourteen patients showed elevated D-dimer and C-reactive protein levels and decreased hemoglobin and albumin levels. Thirteen of the 14 cases were treated surgically, there were no complications of surgery, and 3 patients were readmitted with recurrence of brucellosis, it is related to the treatment that did not reach the early stage, combination, full dose and full course of treatment, with a mean recurrence time of 8 months. In the telephone follow-up, the patient's recovery proceeded well. Conclusion: The present study revealed that the recurrence in male gender, advanced age, smoking and irregular treatment is higher. On the contrast, early intervention, a combination of adequate course of anti-infection treatment and timely surgery can improve the prognosis.

2.
J Cell Mol Med ; 27(3): 422-434, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36651446

RESUMO

Idiopathic pulmonary fibrosis (IPF) is a chronic progressive interstitial lung disease that seriously threatens the health of patients. The pathogenesis of IPF is still unclear, and there is a lack of effective therapeutic drugs. Myofibroblasts are the main effector cells of IPF, leading to excessive deposition of extracellular matrix (ECM) and promoting the progression of fibrosis. Inhibiting the excessive activation and relieving autophagy blockage of myofibroblasts is the key to treat IPF. PI3K/Akt/mTOR pathway plays a key regulatory role in promoting fibroblast activation and autophagy inhibition in lung fibrosis. Duvelisib is a PI3K inhibitor that can simultaneously inhibit the activities of PI3K-δ and PI3K-γ, and is mainly used for the treatment of relapsed/refractory chronic lymphocytic leukaemia (CLL) and small lymphocytic lymphoma tumour (SLL). In this study, we aimed to examine the effects of Duvelisib on pulmonary fibrosis. We used a mouse model of bleomycin-induced pulmonary fibrosis to evaluate the effects of Duvelisib on pulmonary fibrosis in vivo and further explored the potential pharmacological mechanisms of Duvelisib in lung fibroblasts in vitro. The in vivo experiments showed that Duvelisib significantly alleviated bleomycin-induced collagen deposition and improved pulmonary function. In vitro and in vivo pharmacological experiments showed that Duvelisib dose-dependently suppressed lung fibroblast activation and improved autophagy inhibition by inhibiting the phosphorylation of PI3K, Akt and mTOR. Our results indicate that Duvelisib can alleviate the severity of pulmonary fibrosis and provide potential drugs for the treatment of pulmonary fibrosis.

3.
Artigo em Inglês | MEDLINE | ID: mdl-36630316

RESUMO

Studies have previously demonstrated that anxious attachment shapes the association between childhood traumatic experiences and somatic pain; however, it remains unclear how this relationship is influenced by anxious attachment in patients with depression. This study investigated how anxious attachment influences the relationship between childhood traumatic experiences and pain symptoms in depressed patients from a social psychological perspective. A total of 139 adult patients with depression participated in this study; the level of depression was assessed by a psychiatric professional. Childhood trauma, pain symptoms, and attachment dimensions were tested by various questionnaires. The moderating role of anxious attachment in the trauma-pain association was examined using the PROCESS Model 1. Our findings showed that in depressed patients, childhood maltreatment had a significant positive impact on the severity of pain ratings. Moreover, anxious attachment influenced the relationship between childhood trauma and pain symptoms. Our study indicated that anxious attachment is not necessarily a negative outcome for depressed patients; moderate levels of anxious attachment alleviate childhood trauma-related pain symptoms in individuals with highly traumatic experiences. Understanding the traumatic experiences and attachment styles of depressed patients with pain complaints can help to develop intervention strategies.

4.
Clin Anat ; 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36692147

RESUMO

The current study aimed to investigate the sagittal morphology of the spinopelvic complex and the components of the lumbar spine in the normal population. In total, 132 adult volunteers were retrospectively evaluated and divided into four groups according to the Roussouly classification. Statistical analysis of radiological parameters, including lumbar lordosis (LL), thoracic kyphosis (TK), pelvic incidence (PI), pelvic tilt (PT), sacral slope (SS), PI-LL, LL-TK, lumbar vertebral lordosis from L1 to L5 (L1L-L5L), the intervertebral angle from L1-L2 to L5-S1 (IVA1-2-IVA5-1), segmental lordosis from L1 to L5 (S1L-S5L), the proportion of L1-L5 (L1%-L5%), the proportion of the intervertebral angle from L1-L2 to L5-S1 (IVA1-2%-IVA5-1%), and proportion of segmental lordosis from L1 to L5 (S1L%-S5L%), was performed. Based on the classification, type II (n = 46) was the most common, followed by type I (n = 39), type III (n = 36), and type IV (n = 11). The quantitative values of the sagittal parameters of the four groups were obtained. Results showed a significant difference in terms of LL, PI, SS, and LL-TK. Further, L1%, L2%, L3%, IVA1-2%, IVA2-3%, S1L%, S2L%, and S3L% had an increasing trend. PI was positively correlated with LL, S1L, S2L, S3L, S4L, S1L%, and S2L%, but not with S5L%. In conclusion, pelvic parameters had a significant effect on lumbar curvature and lordosis distribution. Further, the abovementioned results were beneficial for individual surgical decision-making regarding targeted intervertebral angle, screw-insertion dimension, and rod contouring.

5.
Cancers (Basel) ; 15(2)2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36672503

RESUMO

The encouraging effects of HER2-ADC in patients with HER2-low expression cancers indicated the classical classifications based on positive and negative HER2 might no longer be suitable. However, the biology and prognosis of colorectal cancer patients with different HER2 expression status were still not clear. This is a multi-center retrospective study that included patients with histologically confirmed colorectal cancer and determined HER2 status who received radical surgical resection. HER2 immunohistochemistry (IHC) 1+ and IHC 2+ groups were combined and defined as a HER2-low group because of the concordance of clinicopathological characteristics. As compared with the HER2-high group, both the HER2-zero and the HER2-low group had less tumor with perineural invasion (14.3%, 13.1% vs. 31.6%, p = 0.001 and p < 0.001), less stage III disease (41.8%, 39.9% vs. 56.1%, p = 0.044 and p = 0.022), more RAS/BRAF mutation (52.1%, 49.9% vs. 19.5%, p < 0.001 and p < 0.001) and better disease-free survival (DFS) (3y-DFS rate of 78.7%, 82.4% vs. 59.3%, p < 0.001 and p < 0.001). Multivariate analysis and propensity score matching also revealed that HER2-high expression was an independent prognostic factor of DFS. In conclusion, our study revealed that HER2-low colorectal cancer tumors are close to HER2-zero tumors, but different from HER2-high tumors. The routine examination of HER2 IHC is needed in early-stage colorectal cancer.

6.
Transl Oncol ; 28: 101604, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36542990

RESUMO

Circular RNAs (circRNAs) have been demonstrated to be important regulators in human malignant tumors, including colorectal cancer (CRC). While the role circ-ZEB1 played in CRC remains unclear. In this study, we aim to explore the biological function and the underlying mechanism of circ-ZEB1 in CRC. RNAscope was used to analyze the expression and localization of circ-ZEB1 in CRC tissues. Loss of function experiments were conducted, including CCK-8, transwell assays, flow cytometry analysis, and murine xenograft models, so as to detect the effect of circ-ZEB1 on CRC cells. IC50 assay was used to evaluate the influence of circ-ZEB1 on the chemoresistance of CRC cells. Epithelial-mesenchymal transition (EMT) related markers were detected. The relationship between circ-ZEB1 and miR-200c-5p was investigated by FISH, dual-luciferase reporter assay, and RIP assay. We found in our study that circ-ZEB1 was significantly upregulated in CRC tissues. Downregulation of circ-ZEB1 inhibited cell proliferation, colony formation, as well as cell migration and invasion abilities of CRC cell lines. In vivo experiments indicated that knockdown of circ-ZEB1 suppressed tumorigenesis and distant metastasis of CRC cells in nude mice. What's more, EMT and chemoresistance of CRC cells were also attenuated following circ-ZEB1 knockdown. Mechanistically, we proved that circ-ZEB1 could directly bind with miR-200c and functioned as miR-200c sponge to exert its biological functions in CRC cells. In conclusion, circ-ZEB1 could promote CRC cells progression, EMT, and chemoresistance via acting on miR-200c, elucidating a potential therapeutic target to inhibit CRC progression.

7.
Rev. bras. med. esporte ; 29: e2022_0238, 2023. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1387949

RESUMO

ABSTRACT Introduction High-intensity interval training contributes expressively to physical training and has been gradually applied to the soccer field. This sport has received more attention in colleges and universities in the country. However, studies are needed on the most appropriate training methods to improve the players' ability among college students, increasing the competitive level among athletes. Objective Investigate the influence of high-intensity intermittent training on soccer players' sports performance. Methods 30 student soccer players were selected at a university for six weeks, and four training sessions were held per week. The experimental group performed intermittent high-intensity training for training; the control group used the traditional aerobic resistance training method. For the experiment, indicator information was collected for integration and analysis before the start of activities and at the end of the six weeks. Results High-intensity intermittent training can improve the fitness index and FMS index of players and also can keep the fluctuation level in players' performance higher; the range of performance improvement is considerably larger than the traditional aerobic training method. Conclusion High-intensity intermittent training can improve players' fitness and agility, reduce sports injuries, improve physical reserve, and promote the progress of soccer players' sports achievements. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.


RESUMO Introdução O treinamento intervalado de alta intensidade contribui expressivamente ao treino físico e gradualmente tem sido aplicado no campo de futebol, esporte que tem recebido maior atenção nas faculdades e universidades do país. Porém, são necessários estudos sobre os métodos de treino mais adequado para melhorar a capacidade dos jogadores entre estudantes universitários, aumentando o nível competitivo entre os atletas. Objetivo Investigar a influência do treinamento intermitente de alta intensidade no desempenho esportivo dos jogadores de futebol. Métodos 30 estudantes futebolistas foram selecionados em uma universidade durante seis semanas e quatro sessões de treinamento foram realizadas por semana. O grupo experimental efetuou treinamento intermitente de alta intensidade para treinamento, o grupo controle utilizou o método tradicional de treinamento de resistência aeróbica para treinamento. Para o experimento, foram coletadas informações de indicadores para integração e análise antes do início das atividades e ao final das seis semanas. Resultados O treinamento intermitente de alta intensidade pode melhorar o índice de aptidão física e o índice FMS dos jogadores, também pode manter o nível de flutuação no desempenho dos jogadores mais elevado, a gama de melhoria de desempenho é consideravelmente maior do que o método tradicional de treinamento aeróbico. Conclusão O treinamento intermitente de alta intensidade pode melhorar o condicionamento físico e a agilidade dos jogadores, reduzir lesões esportivas, melhorar a reserva física e promover o progresso das conquistas esportivas dos jogadores de futebol. Nível de evidência II; Estudos terapêuticos - investigação dos desfechos do tratamento.


RESUMEN Introducción El entrenamiento de intervalos de alta intensidad contribuye de forma expresiva al entrenamiento físico y poco a poco se ha ido aplicando al campo de fútbol, un deporte que ha recibido más atención en los colegios y universidades del país. Sin embargo, se necesitan estudios sobre los métodos de entrenamiento más adecuados para mejorar la capacidad de los jugadores entre los estudiantes universitarios, aumentando el nivel competitivo entre los atletas. Objetivo Investigar la influencia del entrenamiento intermitente de alta intensidad en el rendimiento deportivo de los futbolistas. Métodos Se seleccionaron 30 estudiantes de fútbol en una universidad durante seis semanas y se realizaron cuatro sesiones de entrenamiento por semana. El grupo experimental realizó un entrenamiento intermitente de alta intensidad para entrenar, el grupo de control utilizó el método tradicional de entrenamiento aeróbico de resistencia para entrenar. Para el experimento, se recogió información de los indicadores para su integración y análisis antes del inicio de las actividades y al final de las seis semanas. Resultados El entrenamiento intermitente de alta intensidad puede mejorar el índice de aptitud física y el índice FMS de los jugadores, también puede mantener el nivel de fluctuación en el rendimiento de los jugadores más alto, el rango de mejora del rendimiento es considerablemente mayor que el método de entrenamiento aeróbico tradicional. Conclusión El entrenamiento intermitente de alta intensidad puede mejorar la condición física y la agilidad de los jugadores, reducir las lesiones deportivas, mejorar la reserva física y promover el progreso de los logros deportivos de los futbolistas. Nivel de evidencia II; Estudios terapêuticos - investigación de los resultados del tratamiento.

8.
Sensors (Basel) ; 22(23)2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36501941

RESUMO

We demonstrate a narrow-linewidth, high side-mode suppression ratio (SMSR) semiconductor laser based on the external optical feedback injection locking technology of a femtosecond-apodized (Fs-apodized) fiber Bragg grating (FBG). A single frequency output is achieved by coupling and integrating a wide-gain quantum dot (QD) gain chip with a Fs-apodized FBG in a 1-µm band. We propose this low-cost and high-integration scheme for the preparation of a series of single-frequency seed sources in this wavelength range by characterizing the performance of 1030 nm and 1080 nm lasers. The lasers have a maximum SMSR of 66.3 dB and maximum output power of 134.6 mW. Additionally, the lasers have minimum Lorentzian linewidths that are measured to be 260.5 kHz; however, a minimum integral linewidth less than 180.4 kHz is observed by testing and analyzing the power spectra of the frequency noise values of the lasers.

9.
Front Neurorobot ; 16: 1068274, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36531919

RESUMO

In human-robot collaboration scenarios with shared workspaces, a highly desired performance boost is offset by high requirements for human safety, limiting speed and torque of the robot drives to levels which cannot harm the human body. Especially for complex tasks with flexible human behavior, it becomes vital to maintain safe working distances and coordinate tasks efficiently. An established approach in this regard is reactive servo in response to the current human pose. However, such an approach does not exploit expectations of the human's behavior and can therefore fail to react to fast human motions in time. To adapt the robot's behavior as soon as possible, predicting human intention early becomes a factor which is vital but hard to achieve. Here, we employ a recently developed type of brain-computer interface (BCI) which can detect the focus of the human's overt attention as a predictor for impending action. In contrast to other types of BCI, direct projection of stimuli onto the workspace facilitates a seamless integration in workflows. Moreover, we demonstrate how the signal-to-noise ratio of the brain response can be used to adjust the velocity of the robot movements to the vigilance or alertness level of the human. Analyzing this adaptive system with respect to performance and safety margins in a physical robot experiment, we found the proposed method could improve both collaboration efficiency and safety distance.

10.
Am J Alzheimers Dis Other Demen ; 37: 15333175221144367, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36515911

RESUMO

OBJECTIVE: The present study aims to investigate the underlying neurochemical mechanism of physical exercise on striatum synapsis and memory function in vascular dementia model. METHODS: 32 Sprague-Dawley (SD) rats were randomly divided into 4 groups: control group (C group, n = 6), vascular dementia group (Vascular dementia group, n = 7), physical exercise and vascular dementia group (Exe-VD group, n = 6), physical exercise and black group (Exe group, n = 6). 4 weeks of voluntary wheel running were used as pre-exercise training. Vascular dementia model was established by bilateral common carotid arteries occlusion (BCCAo) for 1 week. Passive avoidance test (PAT) were used to test memory function. The level of striatum catecholamine in the microdialysate were detected by enzyme linked immunosorbent assy (ELISA). Golgi staining was used to analyze striatum neuronal spine density. RESULTS: Behavioral data indicated that 4 weeks of physical exercise ameliorated memory impairment in vascular dementia model. Striatum catecholamine level significantly decreased in VD group when compared with C group (P < .001). But this phenomenon can be rescue by physical exercise (P < .001). In addition, compared with C group, neuronal spine density significantly decreased in VD group (P < .01), but 4 weeks of physical exercise can rescue this phenomenon (P < .05). CONCLUSION: 4 weeks of physical exercise improves memory function by mitigate the decline of striatum catecholamine and spine density in VD model.


Assuntos
Demência Vascular , Fármacos Neuroprotetores , Ratos , Animais , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/uso terapêutico , Ratos Sprague-Dawley , Catecolaminas/farmacologia , Catecolaminas/uso terapêutico , Atividade Motora , Modelos Animais de Doenças , Transtornos da Memória/prevenção & controle , Hipocampo
11.
Sci Rep ; 12(1): 21859, 2022 12 17.
Artigo em Inglês | MEDLINE | ID: mdl-36528731

RESUMO

The study aimed to explore predictors of Mycoplasma pneumoniae pneumonia (MPP) in adults and develop a nomogram predictive model in order to identify high-risk patients early. We retrospectively analysed the clinical data of a total of 337 adult patients with community-acquired pneumonia (CAP) and divided them into MPP and non-MPP groups according to whether they were infected with MP. Univariate and multivariate logistic regression analyses were used to screen independent predictors of MPP in adults and to developed a nomogram model. Receiver operating characteristic (ROC) curve, calibration curve, concordance index (C-index), and decision curve analysis (DCA) were used for the validation of the evaluation model. Finally, the nomogram was further evaluated by internal verification. Age, body temperature, dry cough, dizziness, CRP and tree-in-bud sign were independent predictors of MPP in adults (P < 0.05). The nomogram showed high accuracy with C-index of 0.836 and well-fitted calibration curves in both the training and validation sets. The area under the receiver operating curve (AUROC) was 0.829 (95% CI 0.774-0.883) for the training set and 0.847 (95% CI 0.768-0.925) for the validation set. This nomogram prediction model can accurately predict the risk of MPP occurrence in adults, which helps clinicians identify high-risk patients at an early stage and make drug selection and clinical decisions.


Assuntos
Nomogramas , Pneumonia por Mycoplasma , Adulto , Humanos , Mycoplasma pneumoniae , Estudos Retrospectivos , Pneumonia por Mycoplasma/diagnóstico , Área Sob a Curva
12.
BMC Med ; 20(1): 437, 2022 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-36352434

RESUMO

BACKGROUND: Cervical squamous cell carcinoma (SCC) is known to arise through increasingly higher-grade squamous intraepithelial lesions (SILs) or cervical intraepithelial neoplasias (CINs). This study aimed to describe sequential molecular changes and identify biomarkers in cervical malignant transformation. METHODS: Multidimensional data from five publicly available microarray and TCGA-CESC datasets were analyzed. Immunohistochemistry was carried out on 354 cervical tissues (42 normal, 62 CIN1, 26 CIN2, 47 CIN3, and 177 SCC) to determine the potential diagnostic and prognostic value of identified biomarkers. RESULTS: We demonstrated that normal epithelium and SILs presented higher molecular homogeneity than SCC. Genes in the region (e.g., 3q, 12q13) with copy number alteration or HPV integration were more likely to lose or gain expression. The IL-17 signaling pathway was enriched throughout disease progression with downregulation of IL17C and decreased Th17 cells at late stage. Furthermore, we identified AURKA, TOP2A, RFC4, and CEP55 as potential causative genes gradually upregulated during the normal-SILs-SCC transition. For detecting high-grade SIL (HSIL), TOP2A and RFC4 showed balanced sensitivity (both 88.2%) and specificity (87.1 and 90.1%), with high AUC (0.88 and 0.89). They had equivalent diagnostic performance alone to the combination of p16INK4a and Ki-67. Meanwhile, increased expression of RFC4 significantly and independently predicted favorable outcomes in multi-institutional cohorts of SCC patients. CONCLUSIONS: Our comprehensive study of gene expression profiling has identified dysregulated genes and biological processes during cervical carcinogenesis. RFC4 is proposed as a novel surrogate biomarker for determining HSIL and HSIL+, and an independent prognostic biomarker for SCC.


Assuntos
Infecções por Papillomavirus , Displasia do Colo do Útero , Neoplasias do Colo do Útero , Feminino , Humanos , Prognóstico , Biomarcadores Tumorais/metabolismo , Neoplasias do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/patologia , Expressão Gênica , Infecções por Papillomavirus/diagnóstico , Infecções por Papillomavirus/genética , Proteína de Replicação C/genética , Proteína de Replicação C/metabolismo , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Displasia do Colo do Útero/diagnóstico , Displasia do Colo do Útero/genética , Displasia do Colo do Útero/patologia
13.
Front Psychol ; 13: 1029081, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36337470

RESUMO

Previous studies have paid more attention to the impact of non-balanced reciprocity in the organization on employees' behaviors and outcomes, and have expected that the reciprocity norm could improve employees' compliance behavior. However, there are two distinct types of non-balanced reciprocity, and whether generalized reciprocity affects employees' compliance behavior rather than negative reciprocity and its mechanisms has not been further explored so far. Building on the social exchange theory and cognitive appraisal theory, we established and examined a model in a scenario-based experiment across a two-stage survey of 316 participants. In this article, we propose that generalized reciprocity (relative to negative reciprocity) positively influences employees' compliance behavior, and thriving at work mediates its relationship. Furthermore, we argue that the positive association between generalized reciprocity and thriving at work is moderated by the perceived cognitive capabilities of artificial intelligence (AI). This association is amplified for people high in the perceived cognitive capabilities of AI. We also propose that the positive association between thriving at work and compliance behavior is moderated by conscientiousness, such that the association is amplified for people high in conscientiousness. These findings have theoretical and practical implications.

14.
HardwareX ; 12: e00372, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36393916

RESUMO

While for vision and audio the same mass-produced units can be embedded in many different systems from smartphones to robots, tactile sensors have to be built in application-specific shapes and sizes. To use a commercially available tactile sensor, it can be necessary to develop the entire system around an existing sensor model. We present a set of open-source solutions for designing, manufacturing, reading and integrating custom application-specific tactile matrix sensors. Our manufacturing process only requires an off-the-shelf cutting plotter and widely available plastic and metal foils. This allows creating sensors of diverse sizes, shapes, and layouts, which can be adapted to various specific use cases as demonstrated with exemplary robot integrations. For interfacing and readout, we develop an Arduino-like prototype board (Tacduino) with amplifier circuits to ensure good resolution and to suppress crosstalk. As an example, we give step-by-step instructions to build tactile fingertips for the RobotiQ 3-Finger Gripper, and we provide design files for the readout circuit board together with Arduino firmware and driver software. Both, wired and wireless communication between the sensors and a host PC are supported by this system. The hardware was originally presented and investigated in [1].

16.
Int Immunopharmacol ; 113(Pt B): 109427, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36375321

RESUMO

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and fibrotic interstitial lung disease with lesions confined to the lungs and is prevalent in the middle-aged and elderly population. The average survival time after diagnosis of IPF is only 3-5 years, and the mortality rate is higher than that of most tumours. IPF is called a "tumour-like disease". Entrectinib is a new oral formulation developed by Roche and was approved by the FDA to treat a wide variety of tumours. In this study, we explored the potential effects and mechanisms of entrectinib on pulmonary fibrosis in vitro and in vivo. In vivo studies showed that entrectinib is effective in alleviating bleomycin-induced pulmonary fibrosis. In vitro studies demonstrated that entrectinib dose-dependently inhibits TGF-ß1/non-Smad signaling and attenuates TGF-ß1-induced fibroblast activation and epithelial-mesenchymal transition (EMT). In conclusion, entrectinib blocks TGF-ß1-induced lung fibroblast activation and EMT and then attenuates bleomycin-induced pulmonary fibrosis in mice.


Assuntos
Bleomicina , Fibrose Pulmonar Idiopática , Idoso , Camundongos , Humanos , Animais , Bleomicina/efeitos adversos , Fator de Crescimento Transformador beta1/metabolismo , Matriz Extracelular , Transdução de Sinais , Transição Epitelial-Mesenquimal , Fibrose Pulmonar Idiopática/metabolismo , Pulmão/patologia , Fibroblastos , Camundongos Endogâmicos C57BL
17.
Front Genet ; 13: 971588, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36338955

RESUMO

Structural variants (SVs) are one of the main sources of genetic variants and have a greater impact on phenotype evolution, disease susceptibility, and environmental adaptations than single nucleotide polymorphisms (SNPs). However, SVs remain challenging to accurately type, with several detection methods showing different limitations. Here, we explored SVs from 10 different chickens using PacBio technology and detected 49,501 high-confidence SVs. The results showed that the PacBio long-read detected more SVs than Illumina short-read technology genomes owing to some SV sites on chromosomes, which are related to chicken growth and development. During chicken domestication, some SVs beneficial to the breed or without any effect on the genomic function of the breed were retained, whereas deleterious SVs were generally eliminated. This study could facilitate the analysis of the genetic characteristics of different chickens and provide a better understanding of their phenotypic characteristics at the SV level, based on the long-read sequencing method. This study enriches our knowledge of SVs in chickens and improves our understanding of chicken genomic diversity.

18.
Nutrients ; 14(21)2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36364801

RESUMO

An energy-restricted weight-loss approach has limitations when it used in the elderly, especially because of muscle loss. We aimed to assess the effects of whey protein (WP) or WP hydrolysate (WPH) combined with an energy-restricted diet (ERD) on weight reduction and muscle preservation in older women with overweight and obesity. A total of 60 women were randomized to the control (ERD), WP (ERD + 20 g/d WP) or WPH (ERD + 20 g/d WPH) group, using a 1:1:1 allocation ratio. After an 8-week intervention, body composition, gut microbiota, and serum metabolomics changes were compared among the three groups. The reductions in body weight (-1.11 ± 1.11 vs. -2.34 ± 1.35, p < 0.05), BMI (-0.46 ± 0.45 vs. -0.97 ± 0.54, p < 0.05), and body fat (-0.70 ± 0.92 vs. -2.45 ± 1.65, p < 0.01) were higher in the WPH group than in the control group. Body fat (%) was significantly decreased in the two protein groups. Fat-free mass did not significantly change among the three groups. Serum metabolomics showed that the tricarboxylic acid cycle pathway was upregulated in the WPH group. No significant changes in microbiota were observed among the groups. In conclusion, WP or WPH supplementation combined with an energy-restricted diet benefits older women during weight loss. WPH was more effective, possibly due to increased energy metabolism.


Assuntos
Suplementos Nutricionais , Redução de Peso , Humanos , Feminino , Idoso , Proteínas do Soro do Leite , Composição Corporal , Dieta
19.
Sensors (Basel) ; 22(21)2022 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-36366000

RESUMO

As one of the pioneering data representations, the point cloud has shown its straightforward capacity to depict fine geometry in many applications, including computer graphics, molecular structurology, modern sensing signal processing, and more. However, unlike computer graphs obtained with auxiliary regularization techniques or from syntheses, raw sensor/scanner (metric) data often contain natural random noise caused by multiple extrinsic factors, especially in the case of high-speed imaging scenarios. On the other hand, grid-like imaging techniques (e.g., RGB images or video frames) tend to entangle interesting aspects with environmental variations such as pose/illuminations with Euclidean sampling/processing pipelines. As one such typical problem, 3D Facial Expression Recognition (3D FER) has been developed into a new stage, with remaining difficulties involving the implementation of efficient feature abstraction methods for high dimensional observations and of stabilizing methods to obtain adequate robustness in cases of random exterior variations. In this paper, a localized and smoothed overlapping kernel is proposed to extract discriminative inherent geometric features. By association between the induced deformation stability and certain types of exterior perturbations through manifold scattering transform, we provide a novel framework that directly consumes point cloud coordinates for FER while requiring no predefined meshes or other features/signals. As a result, our compact framework achieves 78.33% accuracy on the Bosphorus dataset for expression recognition challenge and 77.55% on 3D-BUFE.


Assuntos
Reconhecimento Facial , Imageamento Tridimensional/métodos
20.
Discov Oncol ; 13(1): 120, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36334145

RESUMO

We previously found that the Rho-associated kinase 1 (ROCK1) activated Cancer-associated fibroblasts (CAFs) to promote LSCC metastasis. Accumulating evidence indicates that pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is an oncogene; however, the crosstalk between ROCK1 and PYROXD1 in LSCC metastasis remains largely unknown. Here, we found that ROCK1 could target PYROXD1. The knockdown of ROCK1 expression reduces the expression of PYROXD1, while the knockdown of PYROXD1 expression did not alter the expression of ROCK1 indicating that ROCK1 is upstream of PYROXD1. Further, LSCC cells cocultured with PYROXD1 knocked-down CAFs exhibited lower proliferation, migration, invasion and metastasis abilities. Conversely, LSCC cells cocultured with PYROXD1-overexpressing CAFs showed opposite results. In conclusion, the crosstalk between ROCK1 and PYROXD1 regulated CAFs activation and promoted LSCC metastasis.

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