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1.
Plant Dis ; : PDIS12192733RE, 2020 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-32441580

RESUMO

Black point is a common disease in wheat all over the world. The disease could downgrade wheat quality and cause human health problems. In this study, 406 wheat cultivars were used to investigate black point resistance. In the field tests, 20, 65.5, and 14.5% of the tested cultivars were resistant, moderately resistant, and susceptible, respectively, suggesting that improving black point resistance is necessary in Chinese wheat breeding. A genome-wide association study (GWAS) identified 386 single-nucleotide polymorphisms (SNPs) significantly related to black point resistance in the tested wheat cultivars, and they were located on all chromosomes. Linkage mapping in a biparental population identified three quantitative trait loci (QTL) for black point resistance-QBP.hau-3A, QBP.hau-6D, and QBP.hau-7D-with 6.76, 7.79, and 8.84% phenotypic variation explained, respectively. Based on both the GWAS and linkage analyses, QBP.hau-6D covered six significant SNPs from the GWAS, and the position of these SNPs indicated that this QTL is a new locus for black point resistance. This study provides valuable germplasm for breeding wheat cultivars with resistance to black point and information for further understanding of molecular and genetic basis of black point resistance.

2.
Sci Data ; 7(1): 113, 2020 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-32265447

RESUMO

As the human population grows from 7.8 billion to 10 billion over the next 30 years, breeders must do everything possible to create crops that are highly productive and nutritious, while simultaneously having less of an environmental footprint. Rice will play a critical role in meeting this demand and thus, knowledge of the full repertoire of genetic diversity that exists in germplasm banks across the globe is required. To meet this demand, we describe the generation, validation and preliminary analyses of transposable element and long-range structural variation content of 12 near-gap-free reference genome sequences (RefSeqs) from representatives of 12 of 15 subpopulations of cultivated Asian rice. When combined with 4 existing RefSeqs, that represent the 3 remaining rice subpopulations and the largest admixed population, this collection of 16 Platinum Standard RefSeqs (PSRefSeq) can be used as a template to map resequencing data to detect virtually all standing natural variation that exists in the pan-genome of cultivated Asian rice.

3.
Arch Med Res ; 51(2): 160-166, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32111500

RESUMO

BACKGROUND: Interleukin-19 (IL-19) has been shown to be involved in coronary artery diseases and atherosclerosis, while its expression in myocardial infarction is poorly understood. In this study, the dynamic increase in circulating IL-19 in acute ST-segment elevation myocardial infarction (STEMI) patients was detected. METHOD: Both plasma IL-19 levels and IL-19 mRNA expression in peripheral blood mononuclear cells (PBMCs) from STEMI patients and chest pain syndrome (CPS) patients were detected at different time points, including 1 d, 3 d, 7 d and 14 d after treatment and on admission. RESULTS: Compared with the CPS patients, IL-19 levels and IL-19 gene expression were significantly increased in STEMI patients and peaked at 1 d. From 1-14 d, refocusing treatment, including emergency percutaneous coronary intervention (PCI) and thrombolysis, markedly reduced IL-19 expression and promoted its recovery; of the treatments, the effect of emergency PCI was most significant. In addition, similar trends were also observed with cTnI, NT-proBNP and C-reactive protein (CRP) levels. Furthermore, correlation analysis showed that IL-19 levels were positively correlated with cTnI, NT-proBNP, CRP levels and left ventricular ejection fraction (LVEF) in STEMI patients. CONCLUSIONS: IL-19 is correlated with the severity of acute myocardial infarction, which may be a new idea for the clinical treatment of myocardial infarction.

4.
Genome Biol Evol ; 12(4): 413-428, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32125373

RESUMO

The Oryza officinalis complex is the largest species group in Oryza, with more than nine species from four continents, and is a tertiary gene pool that can be exploited in breeding programs for the improvement of cultivated rice. Most diploid and tetraploid members of this group have a C genome. Using a new reference C genome for the diploid species O. officinalis, and draft genomes for two other C genome diploid species Oryza eichingeri and Oryza rhizomatis, we examine the influence of transposable elements on genome structure and provide a detailed phylogeny and evolutionary history of the Oryza C genomes. The O. officinalis genome is 1.6 times larger than the A genome of cultivated Oryza sativa, mostly due to proliferation of Gypsy type long-terminal repeat transposable elements, but overall syntenic relationships are maintained with other Oryza genomes (A, B, and F). Draft genome assemblies of the two other C genome diploid species, Oryza eichingeri and Oryza rhizomatis, and short-read resequencing of a series of other C genome species and accessions reveal that after the divergence of the C genome progenitor, there was still a substantial degree of variation within the C genome species through proliferation and loss of both DNA and long-terminal repeat transposable elements. We provide a detailed phylogeny and evolutionary history of the Oryza C genomes and a genomic resource for the exploitation of the Oryza tertiary gene pool.

5.
Curr Microbiol ; 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32123982

RESUMO

A Gram-stain-negative, non-motile and short-rod-shaped bacterium, designated as strain SY7T, was isolated from rhizosphere soil of the mangrove Kandelia obovata of Fugong village, in Zhangzhou, China. The isolate grew at 10-45 °C (optimum 30 °C), pH 6.0-10.0 (optimum pH 7.0) and 0-8% NaCl (optimum 3%, w/v). The 16S rRNA gene sequence and phylogenetic analysis revealed that strain SY7T located within the radiation of genus Nitratireductor and showed the highest sequence similarity of 97.23% to Nitratireductor pacificus MCCC 1A01024T. The DNA G+C content was 64.9%. In silico DNA-DNA hybridization and average nucleotide identity values between strain SY7T with reference strains of N. pacificus MCCC 1A01024T, N. basaltis KCTC 22119T and N. aquibiodomus DSM 15645T were 16.7%, 14.3%, 14.7% and 75.2%, 72.6%, 73.5%, respectively. The major isoprenoid quinone was Q-10. The dominant fatty acids were 11-methyl C18:1ω7c, iso-C17:0, C19:0ω8c cyclo and summed feature 8 (C18:1ω6c/C18:1ω7c), a profile that almost matched the other members of the genus Nitratireductor. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol. On the basis of the phenotypic, phylogenetic and chemotaxonomic analysis, strain SY7T represents a novel species of the genus Nitratireductor, for which the name Nitratireductor mangrovi sp. nov., is proposed. The type strain is SY7T (= KCTC 72110T = MCCC 1K03723T).

6.
Biomed Chromatogr ; 34(5): e4814, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32100317

RESUMO

Lu-Jiao Fang (LJF), a traditional Chinese medicine prescription, can improve the cardiac function of chronic heart failure (CHF) patients; however, knowledge about the cardiac distribution of LJF, especially in CHF animal models, is rather limited. This work aimed to explore the cardiac distribution of LJF in pressure overload-induced CHF rats at the last gavage administration of LJF after 30 weeks of treatment. LC-MS/MS methods for analyzing nine active components (i.e. loganin, hesperidin, epimedin C, icariin, psoralen, isopsoralen, baohuoside I, morroniside and specnuezhenide) of LJF in cardiac tissue samples were established, and the components were then analyzed in left ventricular wall (LVW) and right ventricular wall (RVW) in parallel at same time point postdose for three dose groups. The results showed that most analytical component levels in LVW (hypertrophic myocardium) were only 39-74% of those in RVW (normal myocardium); however, psoralen and isopsoralen levels in LVW were equal to or even greater than the levels in RVW, suggesting that the hypertrophic myocardium tissue affinity of psoralen and isopsoralen might overcome the negative effect of decreased blood flow on distribution. This study indicated that the pathological state may influence drug distribution, and the efficacy of psoralen and isopsoralen for improving CHF deserves further investigation.

7.
Biomed Pharmacother ; 125: 109896, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32007918

RESUMO

Cisplatin (DDP) is the first-line drug for the treatment of gastric cancer (GC). However, DDP resistance is common. Autophagy, which is closely related to chemoresistance, is a process of resolving and recycling proteins and damaged cellular organs. Additionally, O-6-methylguanine-DNA methyltransferase (MGMT) is responsible for alkylating drug resistance. However, the relationship between autophagy and MGMT in response to DDP in GC is still unknown. In the present study, we determined that autophagy induced by DDP decreases chemosensitivity in GC cell lines. DDP may have induced autophagy in GC by inhibiting MGMT to increase autophagy-related gene (ATG) 4B. Inhibition of MGMT-mediated ATG4B suppression resulted in autophagy induction and DDP resistance. In vivo, combined DDP and autophagy inhibitor chloroquine (CQ) enhanced the anti-tumor effect of DDP; additionally, the negative correlation of MGMT and ATG4B was confirmed. High expression of MGMT and low expression of ATG4B were significantly correlated with favorable five-year survival rate (P < 0.05) in 66 clinicopathologically characterized GC cases. Our study demonstrate that DDP inhibits MGMT-mediated autophagy suppression to decrease chemosensitivity in GC, which provides a novel therapeutic strategy to promote DDP chemosensitivity in GC.

8.
Sensors (Basel) ; 20(4)2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32075193

RESUMO

Grasping force control is important for multi-fingered robotic hands to stabilize the grasped object. Humans are able to adjust their grasping force and react quickly to instabilities through tactile sensing. However, grasping force control through tactile sensing with robotic hands is still relatively unexplored. In this paper, we make use of tactile sensing for multi-fingered robot hands to adjust the grasping force to stabilize unknown objects without prior knowledge of their shape or physical properties. In particular, an online detection module based on Deep Neural Network (DNN) is designed to detect contact events and object material simultaneously from tactile data. In addition, a force estimation method based on Gaussian Mixture Model (GMM) is proposed to compute the contact information (i.e., contact force and contact location) from tactile data. According to the results of tactile sensing, an object stabilization controller is then employed for a robotic hand to adjust the contact configuration for object stabilization. The spatio-temporal property of tactile data is exploited during tactile sensing. Finally, the effectiveness of the proposed framework is evaluated in a real-world experiment with a five-fingered Shadow Dexterous Hand equipped with BioTac sensors.

9.
J Clin Lab Anal ; 34(2): e23073, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31628681

RESUMO

OBJECTIVE: To detect the levels of signal transducer and activator of transcription 4 (STAT4) and soluble endoglin (sEng) in preeclampsia patients and analyze the diagnostic values of STAT4 and sEng in preeclampsia. METHODS: Fifty-four pregnant women with preeclampsia from October 2017 to June 2018 were included in this study. Twenty-eight matched healthy pregnant women were set as the control group. The general clinical characteristics were measured. Serum STAT4 and sEng were detected by ELISA. Correlation between STAT4 and sEng, and their diagnostic value in preeclampsia were analyzed. RESULTS: Compared with control, the prothrombin time in preeclampsia was significantly lower, while the mean arterial pressure, 24-hour urine protein, serum creatinine, fibrinogen, and ALT were significantly higher. The circulating levels of STAT4 and sEng were significantly increased in the preeclampsia. The serum levels of STAT4 and sEng in preeclampsia were positively correlated. For the diagnosis of preeclampsia by the serum STAT4, AUC is 0.902, and the sensitivity and specificity are 0.893 and 0.929. By the serum sEng, AUC is 0.873, and the sensitivity and specificity are 0.816 and 0.905. CONCLUSION: The serum levels of STAT4 and sEng were significantly increased in preeclampsia with disease severity status, which have promise as diagnostic markers in preeclampsia.

10.
J Hazard Mater ; 384: 121428, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31699486

RESUMO

Three types of mosquito-repellent incenses including disc, electric liquid and electric mat, were selected to investigate the emission of volatile organic compounds in the respiratory zone during the burning. VOCs were analyzed by GC-MS. Results showed that the average concentration of TVOCs released by using the disc, electric liquid and electric mat mosquito repellent incense types were 7.760 ±â€¯4.724, 3.122 ±â€¯0.866 and 1.192 ±â€¯0.062 mg/m3, respectively. The TVOCs pollution level produced during the burning of different mosquito-repellent incense types was ranked in the order: disc > electric liquid > electric mat. The concentration of TVOCs produced by Q-liquid, L-liquid, Q-disc and L-disc mosquito-repellent incense types could cause discomfort to the human body. Other types of mosquito-repellent incense induced multiple synergistic effects on human response. About 230 kinds of VOCs including 14 types of VOCs, were found in the smoke of mosquito-repellent incense. The number and content of alkanes was the highest, followed by aromatic hydrocarbons and esters. The level of non-carcinogenic health risk presented by exposure to BTEX (toluene, xylene and ethylbenzene) was acceptable. The chronic daily intake of VOCs decreased with age for both males and females, with male exposure being higher than that of the female, except for children.

11.
J Clin Endocrinol Metab ; 105(3)2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31702013

RESUMO

CONTEXT: Gonadotropin-releasing hormone analogues (GnRHa) and recombinant human growth hormone (rhGH) have been widely used to treat idiopathic central precocious puberty (CPP) or early and fast puberty (EFP). However, large-scale studies to evaluate the treatment effects on final adult height (FAH) are still lacking. OBJECTIVE: To assess the effects of long-term treatment for CPP/EFP on FAH and its main influencing factors. DESIGN AND SETTING: Retrospective, multicenter observational study from 1998 to 2017. PARTICIPANTS: Four hundred forty-eight Chinese girls with CPP/EFP received GnRHa and rhGH treatment (n = 118), GnRHa alone (n = 276), or no treatment (n = 54). MAIN OUTCOME MEASURES: FAH, target height (Tht), and predictive adult height (PAH). RESULTS: The height gain (FAH-PAH) was significantly different among the GnRHa and rhGH treatment, GnRHa alone, and no treatment groups (P < 0.05; 9.51 ± 0.53, 8.07 ± 0.37, and 6.44 ± 0.91 cm, respectively). The genetic height gain (FAH-Tht) was 4.0 ± 0.5 cm for the GnRHa + rhGH group and 2.0 ± 0.27 cm for the GnRHa group, while the control group reached their Tht. In addition, 5 critical parameters derived from PAH, bone age, and Tht, showed excellent performance in predicting which patients could gain ≥5 cm (FAH-PAH), and this was further validated using an independent study. CONCLUSIONS: The overall beneficial effect of GnRHa + rhGH or GnRHa on FAH was significant. The control group also reached their genetic target height. Clinicians are recommended to consider both the potential gains in height and the cost of medication.

12.
Cancer Med ; 9(1): 151-159, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31724334

RESUMO

BACKGROUND: Monosialotetrahexosylganglioside (GM1) is a neuroprotective glycosphingolipid that repairs nerves. Oxaliplatin-based chemotherapy is neurotoxic. This study assessed the efficacy of GM1 for preventing oxaliplatin-induced peripheral neurotoxicity (OIPN) in colorectal cancer (CRC) patients receiving oxaliplatin-based chemotherapy. METHODS: In total, 196 patients with stage II/III CRC undergoing adjuvant chemotherapy with mFOLFOX6 were randomly assigned to intravenous GM1 or a placebo. The primary endpoint was the rate of grade 2 or worse cumulative neurotoxicity (NCI-CTCAE). The secondary endpoints were chronic cumulative neurotoxicity (EORTC QLQ-CIPN20), time to grade 2 neurotoxicity (NCI-CTCAE or the oxaliplatin-specific neuropathy scale), acute neurotoxicity (analog scale), rates of dose reduction or withdrawal due to OIPN, 3-year disease-free survival (DFS) and adverse events. RESULTS: There were no significant differences between the arms in the rate of NCI-CTCAE grade 2 or worse neurotoxicity (GM1: 33.7% vs placebo: 31.6%; P = .76) or neuropathy measured by the EORTC QLQ-CIPN20 or time to grade 2 neurotoxicity using NCI-CTCAE and the oxaliplatin-specific neuropathy scale. GM1 substantially decreased participant-reported acute neurotoxicity (sensitivity to cold items [P < .01], discomfort swallowing cold liquids [P < .01], throat discomfort [P < .01], muscle cramps [P < .01]). The rates of dose reduction or withdrawal were not significantly different between the arms (P = .08). The 3-year DFS rates were 85% and 83% in the GM1 and placebo arms, respectively (P = .19). There were no differences in toxicity between the arms. CONCLUSION: Patients receiving GM1 were less troubled by the symptoms of acute neuropathy. However, we do not support the use of GM1 to prevent cumulative neurotoxicity. (ClinicalTrials.gov number, NCT02251977).

13.
ACS Appl Mater Interfaces ; 12(4): 4432-4442, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31838854

RESUMO

It is well-known that the formation of ferroalloy with the addition of the second or third metal during the steel-making process usually can improve the performance of the iron. Inspired by ferroalloy materials, it is speculated that the pore environment, framework charge, and catalytic properties of metal-organic frameworks (MOFs) could be optimized dramatically via the introduction of ferroalloy-like inorganic building blocks. However, different to ferroalloy, the accurate integration of different metals into one MOF platform is still challenging. Herein, taking advantages of the good compatibility for metals in trigonal prismatic trinuclear cluster, a series of Fe-based alloy-like [M3O(O2C)6] motifs (M3 = Fe3, Fe1.5Ni1.5, Fe1.5Co1.5, Fe1.5Ti1.5, FeCoNi, and FeTiCo) are successfully generated, which further lead to a robust Fe-MOF material family (SNNU-5s). These multicomponent MOFs not only provide a good chance to explore the impact of pore environment on gas adsorption/separation but also offer an opportunity to the efficient electrocatalytic reaction directly. Accordingly, compared with the SNNU-5-Fe parent structure, the pore characters of heterometallic SNNU-5 MOFs are clearly regulated by the type of alloy-like building blocks. SNNU-5-FeTi displays more superior gas separation performance for CO2/CH4, C2H2/CH4, C2H4/CH4, and C2H2/CO2 gas mixtures. What is more, benefited from the multimetallic active sites and their catalytic synergy, FeCoNi-ternary alloy-like cluster-based SNNU-5 MOF material exhibits an exceptional oxygen evolution reaction activity in aqueous solution at pH = 13, which delivers a low overpotential (ηj=10 = 317 mV), a fast reaction kinetics (Tafel slope = 37 mV dec-1), and excellent catalytic stability. This facile multialloy-like building block strategy holds promise to accurately design and improve the performance of MOFs, as well as open an avenue to understand the related mechanisms.

14.
Angew Chem Int Ed Engl ; 59(14): 5647-5650, 2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-31859408

RESUMO

A highly atroposelective (up to 97 % ee) Au-catalyzed synthesis of 1,1'-binaphthalene-2,3'-diols is reported starting from a range of substituted benzyl alkynones. Essential for the achievement of high enantioselectivity during the key assembly of the naphto-3-ol unit is the use of TADDOL-derived α-cationic phosphonites as ancillary ligands. Preliminary results demonstrate that the transformation of the obtained binaphthyls into axially chiral monodentate phosphines is possible without degradation of enantiopurity.

15.
Inorg Chem ; 58(24): 16792-16799, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31762269

RESUMO

Evaluating the effect of ligand substitution on metal ions and/or clusters during the MOF growth process is conducive to rational design of isoreticular MOFs with improved performance. Through topological direction and ligand substitution strategy, we herein constructed two Sc-soc-MOFs (Sc-EBTC and Sc-ABTC) based on two similar rectangular-planar diisophthalate ligands, linear-shaped H4EBTC (1,1'-ethynebenzene-3,3',5,5'-tetracarboxylic acid) and zigzag-shaped H4ABTC (3,3',5,5'-azobenzenetetracarboxylic acid), under solvothermal conditions with formic acid as a modulator. {Sc[(Sc3O)(H2O)3]3(EBTC)6} (Sc-EBTC) possesses two distinct clusters as SBUs, trinuclear [Sc3O(CO2)6] (SBU1) and mononuclear cluster [ScO6] (SBU2), which maintain the soc-topology except for the mononuclear [ScO6] instead of the corresponding trinuclear [Sc3O(CO2)6] in Sc-ABTC ({(Sc3O)(H2O)3(ABTC)1.5(NO3)}). Notably, Sc-EBTC represents a rare soc-MOF with two distinct clusters as SBUs. Due to similar pore spaces, the two Sc-soc-MOF materials both exhibit enhanced and comparable gas sorption and selectivity performances. Specially, their remarkable C2H2, C2H4, and CO2 storage capacity along with prominent CO2/CH4 and C2-hydrocarbons/CH4 separations indicate that these Sc-soc-MOFs are promising adsorbents for natural gas purification under ambient conditions.

16.
ACS Sens ; 4(12): 3175-3185, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31670508

RESUMO

Wireless implantable neural interfaces can record high-resolution neuropotentials without constraining patient movement. Existing wireless systems often require intracranial wires to connect implanted electrodes to an external head stage or/and deploy an application-specific integrated circuit (ASIC), which is battery-powered or externally power-transferred, raising safety concerns such as infection, electronics failure, or heat-induced tissue damage. This work presents a biocompatible, flexible, implantable neural recorder capable of wireless acquisition of neuropotentials without wires, batteries, energy harvesting units, or active electronics. The recorder, fabricated on a thin polyimide substrate, features a small footprint of 9 mm × 8 mm × 0.3 mm and is composed of passive electronic components. The absence of active electronics on the device leads to near zero power consumption, inherently avoiding the catastrophic failure of active electronics. We performed both in vitro validation in a tissue-simulating phantom and in vivo validation in an epileptic rat. The fully passive wireless recorder was implanted under rat scalp to measure neuropotentials from its contact electrodes. The implanted wireless recorder demonstrated its capability to capture low voltage neuropotentials, including somatosensory evoked potentials (SSEPs), and interictal epileptiform discharges (IEDs). Wirelessly recorded SSEP and IED signals were directly compared to those from wired electrodes to demonstrate the efficacy of the wireless data. In addition, a convoluted neural network-based machine learning algorithm successfully achieved IED signal recognition accuracy as high as 100 and 91% in wired and wireless IED data, respectively. These results strongly support the fully passive wireless neural recorder's capability to measure neuropotentials as low as tens of microvolts. With further improvement, the recorder system presented in this work may find wide applications in future brain machine interface systems.

17.
Appl Opt ; 58(28): 7774-7780, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31674459

RESUMO

A compact, low-cost, and highly sensitive optical fiber hydrophone (OFH) based on an incident-angle sensing mechanism (IA-OFH) was demonstrated. An off-center positioned collimator was used for light emitting and receiving, which is very sensitive to the incident angle due to the very small beam divergence of the collimator. Moreover, owing to the angle-sensitive detection (without using any optical interference effect), precise control on the light wavelength and the cavity length is no longer needed, which can greatly simplify the device fabrication and the interrogation system. A quartz IA-OFH with a detection limit of 0.7 mPa/Hz1/2, a dynamic range of ∼110 dB, and a response bandwidth over 1.0 MHz was experimentally demonstrated.

18.
Oncoimmunology ; 8(12): e1663108, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31741760

RESUMO

Introduction: PD-1 inhibitors have been approved for the treatment of dMMR patients with metastatic colorectal cancer, but the efficacy of neoadjuvant treatment with PD-1 in dMMR locally advanced rectal cancer (LARC) patients has not yet been defined. Patients and methods: Two patients with LARC received Nivolumab as neoadjuvant treatment in July 2017. Whole-exome sequencing (WES) and multiplex immunofluorescence analysis were performed. Results: Of the two patients, one achieved pathological complete response after six cycles of nivolumab followed by surgery. The other patient was confirmed to be clinical complete response after six cycles of nivolumab. "Watch and wait" strategy was performed for anal preservation. WES showed high tumor mutation burden. Multiplex immunofluorescence analysis showed immune microenvironment alternation between pretreatment specimen and post-treatment specimen. Conclusion: Neoadjuvant nivolumab induced complete response in both of the two patients with LARC. Immunotherapy might be an alternative strategy for neoadjuvant treatment for dMMR/MSI rectal cancer.

19.
Nanoscale Res Lett ; 14(1): 345, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31754842

RESUMO

Prepared composite materials based on [Zn4O(benzene-1,4-dicarboxylate)3] (MOF-5) and graphene oxide (GO) via a simple green solvothermal method, at which GO was used as platform to load MOF-5, and applied to the thermal decomposition of AP. The obtained composites were characterized by various techniques such as scanning electron microscopy (SEM), X-ray diffraction (XRD), nitrogen adsorption, Fourier transform infrared (FT-IR), differential scanning calorimetry and thermalgravimetric (DSC-TG). The analyses confirmed that the composite material (GO@) MOF-5 can not only improve the decomposition peak temperature of AP from the initial 409.7 °C to 321.9 °C, but also can improve the enthalpy (△H) from 576 J g-1 to 1011 J g-1 and reduce the activation energy (Ea), thereby accelerating the decomposition reaction. The high-specific surface area of the MOF material can provide a large number of active sites, so that the transition metal ions supported thereon can participate more effectively in the electron transfer process, and GO plays its role as a bridge by its efficient thermal and electrical conductivity. Together, accelerate the thermal decomposition process of AP.

20.
Medicine (Baltimore) ; 98(40): e17244, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31577716

RESUMO

RATIONALE: Gitelman syndrome (GS) is a rare autosomal recessive hereditary salt-losing tubulopathy caused by loss-of-function mutations in the SLC12A3 gene. It is usually characterized by hypokalemia, metabolic alkalosis, hypomagnesemia, and hypocalciuria. There are only a few reports on GS combined with growth hormone deficiency (GHD). PATIENT CONCERNS: Three patients presented with weakness, spasm, and growth retardation, respectively. DIAGNOSES: GS was diagnosed based on the clinical symptoms, laboratory test results, and genetic analysis. GH stimulation tests were performed when the magnesium level returned to normal under magnesium oxide (MgO) therapy. INTERVENTIONS: Initially, all patients received oral replacement of MgO and potassium chloride, and 2 of them received simultaneous spironolactone therapy. Recombinant human growth hormone (rhGH) therapy was initiated after they were diagnosed with GHD. OUTCOMES: All 3 patients exhibited satisfactory growth velocity and normal serum magnesium level, although the potassium level was still slightly lower than normal. LESSONS: We suggest that all GS patients should undergo genetic evaluation, especially regarding SLC12A3 gene mutation. GHD should be considered if these patients have short stature. rhGH therapy is useful for stimulating the patients' growth, and it may increase the serum magnesium level.


Assuntos
Síndrome de Gitelman/complicações , Hormônio do Crescimento/deficiência , Criança , Pré-Escolar , Feminino , Síndrome de Gitelman/genética , Hormônio do Crescimento Humano/uso terapêutico , Humanos , Óxido de Magnésio/uso terapêutico , Masculino , Cloreto de Potássio/uso terapêutico , Membro 3 da Família 12 de Carreador de Soluto/genética , Espironolactona/uso terapêutico
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