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1.
Sensors (Basel) ; 22(14)2022 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-35890798

RESUMO

Effective denoising can ensure fast and accurate target detection. This paper presents an electric field measurement system based on a high-speed motion platform, which was built to analyze the characteristics of low frequency electric field noise. An offshore test has shown that it is possible to detect a low-frequency electric field using a high-speed motion platform. Low frequency electric field noise was then collected to analyze its characteristics in terms of time and frequency domains. Based on the noise characteristics, complete ensemble empirical mode decomposition with adaptive noise (ICEEMDAN) was improved and combined with an adaptive threshold algorithm for denoising and reconstructing target containing noise signals. As revealed in the results, the proposed algorithm achieved highly effective denoising to overcome the line spectrum detection failure resulting from a high-speed motion platform. The detection range had also been improved from the original 853 m to 1306 m, a 53.1% increase.


Assuntos
Algoritmos , Ruído , Razão Sinal-Ruído
2.
CRISPR J ; 2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35900273

RESUMO

B lymphocytes are activated and regulated by their interactions with T cells, a process that results in one-way class switching of immunoglobulins (ig) from IgM to IgG, IgE, or IgA. In this study, we show the application of clustered regularly interspaced short palindromic repeat-Cas9-induced nonhomologous end joining in B cells to achieve reverse-directional Ig class switching. By electroporating Cas9 and guide RNA and a Cµ encoding donor into cells, we engineered IgG-secreting human B cell lines to switch to express IgM antibody. This approach offers a new potential path for the production of IgM antibodies.

3.
J Neuroinflammation ; 19(1): 189, 2022 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-35842640

RESUMO

BACKGROUND: Nafamostat mesylate (nafamostat, NM) is an FDA-approved serine protease inhibitor that exerts anti-neuroinflammation and neuroprotective effects following rat spinal cord injury (SCI). However, clinical translation of nafamostat has been limited by an unclear administration time window and mechanism of action. METHODS: Time to first dose of nafamostat administration was tested on rats after contusive SCI. The optimal time window of nafamostat was screened by evaluating hindlimb locomotion and electrophysiology. As nafamostat is a serine protease inhibitor known to target thrombin, we used argatroban (Arg), a thrombin-specific inhibitor, as a positive control in the time window experiments. Western blot and immunofluorescence of thrombin expression level and its enzymatic activity were assayed at different time points, as well its receptor, the protease activated receptor 1 (PAR1) and downstream protein matrix metalloproteinase-9 (MMP9). Blood-spinal cord barrier (BSCB) permeability leakage indicator Evans Blue and fibrinogen were analyzed along these time points. The infiltration of peripheral inflammatory cell was observed by immunofluorescence. RESULTS: The optimal administration time window of nafamostat was 2-12 h post-injury. Argatroban, the thrombin-specific inhibitor, had a similar pattern. Thrombin expression peaked at 12 h and returned to normal level at 7 days post-SCI. PAR1, the thrombin receptor, and MMP9 were significantly upregulated after SCI. The most significant increase of thrombin expression was detected in vascular endothelial cells (ECs). Nafamostat and argatroban significantly downregulated thrombin and MMP9 expression as well as thrombin activity in the spinal cord. Nafamostat inhibited thrombin enrichment in endothelial cells. Nafamostat administration at 2-12 h after SCI inhibited the leakage of Evans Blue in the epicenter and upregulated tight junction proteins (TJPs) expression. Nafamostat administration 8 h post-SCI effectively inhibited the infiltration of peripheral macrophages and neutrophils to the injury site. CONCLUSIONS: Our study provides preclinical information of nafamostat about the administration time window of 2-12 h post-injury in contusive SCI. We revealed that nafamostat functions through inhibiting the thrombin-mediated BSCB breakdown and subsequent peripheral immune cells infiltration.


Assuntos
Metaloproteinase 9 da Matriz , Traumatismos da Medula Espinal , Animais , Benzamidinas , Barreira Hematoencefálica/metabolismo , Células Endoteliais/metabolismo , Azul Evans/metabolismo , Azul Evans/farmacologia , Guanidinas , Metaloproteinase 9 da Matriz/metabolismo , Ratos , Ratos Sprague-Dawley , Receptor PAR-1/metabolismo , Inibidores de Serino Proteinase/farmacologia , Inibidores de Serino Proteinase/uso terapêutico , Medula Espinal , Traumatismos da Medula Espinal/tratamento farmacológico , Traumatismos da Medula Espinal/metabolismo , Trombina/metabolismo
4.
Front Immunol ; 13: 927761, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35844530

RESUMO

The need to be diagnosed with liver biopsy makes the clinical progression of chronic HBV infection diagnosis a challenge. Existing HBV serum biochemical assays are used throughout clinical but have limited effects. Studies have shown that mitochondrial function is tightly coupled to HBV infection. Here, we verified the diagnostic value of serum Adenosine Triphosphate (ATP) as a potential marker for differential HBV infection progress by detecting the level of ATP in the serum from a wide spectrum of HBV-infected populations, and confirmed the role of ATP in the deterioration of HBV infection-related diseases through HBV-infected cells and mouse models. The results showed that there were significantly lower serum ATP levels in HBeAg-positive CHB patients compared with healthy controls. And during the progression of CHB to liver cirrhosis and hepatocellular carcinoma, the ATP level was increased but not higher than healthy controls. The area under the curve (AUC) of serum ATP was 0.9063 to distinguish HBeAg-positive CHB from healthy, and another AUC was 0.8328 in the CHB against the HCC group. Preliminary exploration of the mechanism indicated that the decline of serum ATP was due to impaired mitochondria in CHB patients. Our data provide evidence that serum ATP distinguishes the various progress of HBV infection-related diseases and expands diagnostic biomarkers for HBeAg-positive CHB patients with healthy controls.


Assuntos
Carcinoma Hepatocelular , Hepatite B Crônica , Hepatite B , Neoplasias Hepáticas , Trifosfato de Adenosina , Animais , Biomarcadores , Carcinoma Hepatocelular/diagnóstico , Diagnóstico Diferencial , Progressão da Doença , Hepatite B/diagnóstico , Antígenos E da Hepatite B , Vírus da Hepatite B , Hepatite B Crônica/patologia , Neoplasias Hepáticas/diagnóstico , Camundongos
5.
ACS Appl Mater Interfaces ; 14(27): 31024-31034, 2022 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-35776768

RESUMO

N-type Mg3(Sb,Bi)2 compounds have recently been demonstrated as promising low-cost efficient thermoelectric materials in low and intermediate temperature ranges; however, the thermal stability of this type of material still poses a great challenge for practical applications. In this work, we conduct a systematic investigation of the thermal stability of several high-performing n-type Mg3(Sb,Bi)2-based thermoelectric materials in both bulk and powdered forms using X-ray and neutron diffraction. It is found that the bulk sample exhibits a much slower degradation rate based on the evolution of the secondary Bi/Sb phase in comparison with the powdered sample, revealing a clear kinetic effect. Moreover, the surface of the bulk sample will gradually become Mg-poor or Bi-rich even at room temperature when exposed to air for a long time, highlighting the importance of surface encapsulation for applications. An underlying mechanism based on the Mg loss/migration is proposed to account for the property degradation. Importantly, to address the property degradation, we discuss possible solutions and propose Mg-vapor annealing as an effective approach to enhance thermal stability by suppressing the Mg loss/migration through saturating grains and grain boundaries with elemental Mg. We expect a combination of the Mg-vapor annealing and surface coating to further improve the long-term thermal stability. This work sheds light on the strategies for enhancing the long-term stability of n-type Mg3Sb2-based thermoelectrics for practical applications.

6.
Food Funct ; 2022 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-35904366

RESUMO

Strenuous exercise is reported to provoke deleterious consequences including cardiac impairments, while the detailed mechanisms and effective interventions remain limited. The current study aims to explore the profitable effects of hydroxytyrosol (HT), one of the most abundant polyphenols derived from olive oil, on strenuous exercise-induced pathological changes in the heart and its underlying mechanisms. Sprague-Dawley male rats at the age of 8-week-old were supplemented with 25 mg kg-1 day-1 of HT 45 min before the beginning of strenuous exercise for a total of 8 weeks. HT treatment obviously improved the heart weight and morphology with lowered serum cardiac hypertrophy markers as well as cardiac oxidative stress. Moreover, the down-regulated mitochondrial biogenesis pathway, impaired mitochondrial complex activity, dysregulated expression of mitochondrial dynamics-related proteins and activated apoptotic pathway induced by Exe were all improved by HT. In vitro, 10 µM HT effectively reduced the reactive oxygen species level, promoted mitochondrial biogenesis, and inhibited apoptosis and cardiomyocyte hypertrophy in an angiotensin II-induced cardiomyocyte hypertrophy model. In addition, knockdown of the peroxisome proliferator-activated receptor gamma coactivator-1 alpha, the key regulator of mitochondrial biogenesis, partially abolished the benefits of HT. Our results demonstrate that the disturbance of mitochondrial homeostasis plays a substantial role in strenuous exercise-induced pathological cardiac hypertrophy, and HT presents as an effective intervention strategy targeting mitochondrial homeostasis for cardiac health.

7.
Plants (Basel) ; 11(13)2022 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-35807578

RESUMO

As a source of aromatic plants, the genus Artemisia has long been considered to have the potential to develop plant pesticides. In this study, components of essential oils from A. dalai-lamae, A. tangutica, A. sieversiana, A. tanacetifolia and A. ordosica were identified by GC-MS. A total of 56 constituents were analysed, and each species consisted of 9 to 24 constituents. Principle component analysis (PCA) revealed that A. dalai-lamae, A. tangutica and A. tanacetifolia are characterised by monoterpene hydrocarbons and oxygenated monoterpenes. Hierarchical cluster analysis (HCA) showed the most remarkable similarity between A. sieversiana and A. ordosica, but the similarity was still lower than 50%. Contact toxicity and repellency of essential oils were evaluated by bioassays; A. ordosica oil exhibited the most substantial contact toxicity (LD50 = 52.11 µg/cm2) against Liposcelis bostrychophila, while A. tangutica oil showed the most potent contact toxicity (LD50 = 17.42 µg/adult) against Tribolium castaneum. Except for A. dalai-lamae, the other four species showed the same level (p > 0.05) of repellent activity as the positive control against both pests at high concentrations. The results indicated that these five Artemisia species had high chemical diversity and great potential to be developed into more effective and environmentally friendly anti-insect agents.

8.
Sheng Wu Gong Cheng Xue Bao ; 38(7): 2513-2522, 2022 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-35871621

RESUMO

A bio-electrochemical system can promote the interaction between microorganism and electrode and consequently change cellular metabolism. To investigate the metabolic performance of Zymomonas mobilis in the bio-electrochemical system, we applied an H-type bio-electrochemical reactor to control Z. mobilis fermentation under 3 V. Compared with the control group without applied voltage, the glycerol in the anode chamber increased by 24%, while the glucose consumption in the cathode chamber increased by 16%, and the ethanol and succinic acid concentration increased by 13% and 8%, respectively. Transcriptomic analysis revealed that the pathways related to organic acid metabolism, redox balance, and electron transfer played roles in metabolic changes. Three significantly differentially expressed genes, ZMO1060 (superoxide dismutase), ZMO0401 (diguanylate cyclase), and ZMO1819 (nitrogen fixation protein), were selected to verify their functions in the bio-electrochemical system. Overexpression of ZMO1060 and ZMO1819 improved the electrochemical activity of Z. mobilis. This study provides insights into the microbial metabolism regulated by the bio-electrochemical system.


Assuntos
Zymomonas , Etanol/metabolismo , Fermentação , Glucose/metabolismo , Ácido Succínico/metabolismo , Zymomonas/genética , Zymomonas/metabolismo
9.
BMC Cancer ; 22(1): 806, 2022 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-35864459

RESUMO

BACKGROUND: For colorectal cancer, preoperative (neoadjuvant) chemotherapy is more effective than postoperative chemotherapy because it not only eradicates micrometastases more effectively but also reduces the risk of incomplete intraoperative resection and tumor cell shedding. For the treatment of acute left-sided malignant colorectal obstruction, colorectal stents as well as stoma are being used to relieve the obstructive colorectal cancer, and as a bridge to surgery, allowing easy mobilization and resection of the colon. Neoadjuvant chemotherapy combined with self-expandable metal stents (SEMS) or neoadjuvant chemotherapy combined with decompressing stoma (DS) can be used as a bridge to elective surgery (BTS) as an alternative to emergency surgery in patients with acute left-sided malignant colorectal obstruction, but its benefit is uncertain. The purpose of this study was to evaluate the safety and feasibility of neoadjuvant chemotherapy as a bridge to surgery in the treatment of acute left-sided malignant colorectal obstruction. METHODS: Data from patients who were admitted with acute left-sided malignant colorectal obstruction between January 2012 and December 2020 were retrospectively reviewed, and patients with gastrointestinal perforation or peritonitis were excluded. We performed one-to-two propensity score matching to compare the stoma requirement, postoperative complications, and other short-term oncological outcomes between the neoadjuvant chemotherapy group and surgery group. RESULTS: There were no differences in intraoperative blood loss, operative time, one-year postoperative mortality, and postoperative tumor markers between the two groups. The 1-year recurrence-free survival (RFS) rates of neoadjuvant chemotherapy group and surgery group were 96.8 and 91.3% (p = 0.562). The neoadjuvant chemotherapy group was able to reduce stoma rate 1 year after surgery (p = 0.047). Besides, the neoadjuvant group significantly reduced postoperative bowel function time (p < 0.001), postoperative hospital stay (p < 0.001), total hospital stay (p = 0.002), postoperative complications (p = 0.017), reduction in need to stay in the intensive care unit (ICU) (p = 0.042). CONCLUSIONS: Neoadjuvant chemotherapy as a bridge to elective surgery in patients with acute left-sided malignant colorectal obstruction is safe and has many advantages. Prospective multicenter studies with large samples are needed to further evaluate the feasibility of neoadjuvant chemotherapy.


Assuntos
Neoplasias Colorretais , Obstrução Intestinal , Neoplasias Colorretais/complicações , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/cirurgia , Estudos de Viabilidade , Humanos , Obstrução Intestinal/etiologia , Obstrução Intestinal/patologia , Obstrução Intestinal/cirurgia , Terapia Neoadjuvante/efeitos adversos , Complicações Pós-Operatórias , Estudos Prospectivos , Estudos Retrospectivos , Resultado do Tratamento
10.
Dalton Trans ; 2022 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-35876155

RESUMO

Fluorescent materials have gradually become a hot spot in the field of anti-counterfeiting. Multifunctional phosphors used in anti-counterfeiting designs still have the problems of disordered reading sequences, difficulty in detection, and easy forging. To resolve these problems, we propose a new flexible dual-mode anti-counterfeiting design using a series of phosphors Ca3Al2Ge3O12:Cr3+ (CAG) with deep red persistent luminescence peaking at 722 nm. By adjusting the doping concentration of Cr3+ from 2% to 6%, deep red persistent luminescence with different afterglow intensities and durations can be achieved. By performing a series of thermoluminescence (TL) experiments under different conditions, the defects in materials were comprehensively and systematically analyzed. The defects contributed to the deep and shallow traps; this led to an obvious improvement in its long persistent luminescence (LPL). Such a dual-mode system with flexibility and simplicity properties is a good choice not only for anti-counterfeiting, but also for multi-layer information encryption, and a series of demo experiments based on the digital tube, Moss code, QR code, bar-code, school celebration pattern, and love letter information encryption design were implemented. Their dynamic anti-counterfeiting applications have been demonstrated, which provides a new way to rationally design multi-functional luminescent materials.

11.
Polymers (Basel) ; 14(13)2022 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-35808628

RESUMO

The defect and N-doping engineering are critical to developing the highly efficient metal-free electrocatalysts for oxygen reduction reaction (ORR), mainly because they can efficiently regulate the geometric/electronic structures and sur-/interface properties of the carbon matrix. Herein, we provide a facile and scalable strategy for the large-scale synthesis of N-doped porous carbon nanosheets (NPCNs) with hierarchical pore structure, only involving solvothermal and pyrolysis processes. Additionally, the turnover frequency of ORR (TOFORR) was calculated by taking into account the electron-transfer number (n). Benefiting from the trimodal pore structures, high specific surface area, a higher pore volume, high-ratio mesopores, massive vacancies/long-range structural defects, and high-content pyridinic-N (~2.1%), the NPCNs-1000 shows an excellent ORR activity (1600 rpm, js = ~5.99 mA cm-2), a selectivity to four-electron ORR (~100%) and a superior stability in both the three-electrode tests (CP test for 7500 s at 0.8 V, Δjs = ~0.58 mA cm-2) and Zn-Air battery (a negligible loss of 0.08 V within 265 h). Besides, the experimental results indicate that the enhancement of ORR activity mainly originates from the defects and pyridinic-N. More significantly, this work is expected to realize green and efficient energy storage and conversion along with the carbon peaking and carbon neutrality goals.

12.
Front Cell Infect Microbiol ; 12: 932204, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35811681

RESUMO

SARS-CoV-2 breakthrough infections have been reported because of the reduced efficacy of vaccines against the emerging variants globally. However, an accurate model to predict SARS-CoV-2 breakthrough infection is still lacking. In this retrospective study, 6,189 vaccinated individuals, consisting of SARS-CoV-2 test-positive cases (n = 219) and test-negative controls (n = 5970) during the outbreak of the Delta variant in September 2021 in Xiamen and Putian cities, Fujian province of China, were included. The vaccinated individuals were randomly split into a training (70%) cohort and a validation (30%) cohort. In the training cohort, a visualized nomogram was built based on the stepwise multivariate logistic regression. The area under the curve (AUC) of the nomogram in the training and validation cohorts was 0.819 (95% CI, 0.780-0.858) and 0.838 (95% CI, 0.778-0.897). The calibration curves for the probability of SARS-CoV-2 breakthrough infection showed optimal agreement between prediction by nomogram and actual observation. Decision curves indicated that nomogram conferred high clinical net benefit. In conclusion, a nomogram model for predicting SARS-CoV-2 breakthrough infection based on the real-world setting was successfully constructed, which will be helpful in the management of SARS-CoV-2 breakthrough infection.


Assuntos
COVID-19 , COVID-19/diagnóstico , COVID-19/epidemiologia , Humanos , Nomogramas , Estudos Retrospectivos , SARS-CoV-2
13.
EBioMedicine ; 82: 104087, 2022 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-35797768

RESUMO

BACKGROUND: Cold exposure is one of the most important risk factors for atrial fibrillation (AF), and closely related to the poor prognosis of AF patients. However, the mechanisms underlying cold-related AF are poorly understood. METHODS: Various techniques including 16S rRNA gene sequencing, fecal microbiota transplantation, and electrophysiological examination were used to determine whether gut microbiota dysbiosis promotes cold-related AF. Metabonomics were performed to investigate changes in fecal trimethylamine (TMA) and plasma trimethylamine N-oxide (TMAO) during cold exposure. The detailed mechanism underlying cold-related AF were examined in vitro. Transgenic mice were constructed to explore the role of pyroptosis in cold-related AF. The human cohort was used to evaluate the correlation between A. muciniphila and cold-related AF. FINDINGS: We found that cold exposure caused elevated susceptibility to AF and reduced abundance of Akkermansia muciniphila (A. muciniphila) in rats. Intriguingly, oral supplementation of A. muciniphila ameliorated the pro-AF property induced by cold exposure. Mechanistically, cold exposure disrupted the A. muciniphila, by which elevated the level of trimethylamine N-oxide (TMAO) through modulation of the microbial enzymes involved in trimethylamine (TMA) synthesis. Correspondingly, progressively increased plasma TMAO levels were validated in human subjects during cold weather. Raised TMAO enhanced the infiltration of M1 macrophages in atria and increased the expression of Casp1-p20 and cleaved-GSDMD, ultimately causing atrial structural remodeling. Furthermore, the mice with conditional deletion of caspase1 exhibited resistance to cold-related AF. More importantly, a cross-sectional clinical study revealed that the reduction of A. muciniphila abundance was an independent risk factor for cold-related AF in human subjects. INTERPRETATION: Our findings revealed a novel causal role of aberrant gut microbiota and metabolites in pathogenesis of cold-related AF, which raises the possibility of selectively targeting microbiota and microbial metabolites as a potential therapeutic strategy for cold-related AF. FUNDING: This work was supported by grants from the State Key Program of National Natural Science Foundation of China (No.81830012), and National Natural Science Foundation of China (No.82070336, No.81974024), Youth Program of the National Natural Science Foundation of China (No.81900374, No.81900302), and Excellent Young Medical Talents supporting project in the First Affiliated Hospital of Harbin Medical University (No. HYD2020YQ0001).

14.
Environ Res ; 213: 113637, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35688221

RESUMO

Power plants emit sulfur dioxide (SO2) during combustion, which is typically removed via wet flue gas desulfurization, but this process produces numerous secondary pollutants. Ionic liquids (ILs) can potentially be used to remove SO2, but they suffer from poor mass transfer rates. Hydroxyl ammonium ILs are classical cheap ILs that contain electron-rich O and N sites that favor high absorption capacities. To accelerate mass transfer, two hydroxyl ammonium ILs, triethanolamine citrate and triethanolamine lactate, were immobilized on activated carbon (SILs) and used to capture SO2 from simulated flue gas. They exhibited excellent adsorption at low SO2 partial pressures due to the presence of a large gas-liquid interface. The molar adsorption ratios reached 7.65 and 2.40 mol/mol at 10 kPa SO2. The SILs possessed good SO2 selectivity in SO2/CO2 and SO2/O2 mixtures, because of the only 8% reduction in the total adsorption of SILs at 60 °C. And they exhibited excellent reversibility in which their total adsorption capacities were unaffected after 5 adsorption-desorption cycles. The mechanism analysis revealed that chemical adsorption was the major adsorption route, although physical adsorption also occurred. The main reactive sites included C-O and N-H groups in the ionic liquid. These SILs may potentially replace traditional chemical absorption materials for the separation of SO2 from flue gas.


Assuntos
Poluentes Atmosféricos , Compostos de Amônio , Líquidos Iônicos , Poluentes Atmosféricos/análise , Compostos de Amônio/análise , Carvão Vegetal , Radical Hidroxila/análise , Líquidos Iônicos/química , Dióxido de Enxofre/análise
15.
Free Radic Biol Med ; 188: 194-205, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35750271

RESUMO

BACKGROUND: Inflammation plays an important role in diabetes mellitus (DM)-related acute ischemic stroke (AIS). The mechanisms of un-resolved inflammation in DM-related AIS are not fully understood. Specialized pro-resolving mediators (SPMs) are key regulators that promote resolution of inflammation. We aimed to examine resolution function in patients with AIS complicated with DM, and explore potential treatment effects of one of the SPMs, resolvin D2 (RvD2) ex vivo and in vivo. METHODS: Cultured human macrophages, which were derived from peripheral blood mononuclear cells of AIS and none-AIS patients with or without DM, were stimulated with oxidized-low density lipoprotein (ox-LDL). Levels of SPMs and inflammatory markers were analysed, and RvD2 treatment effects were evaluated in these cells. For experiments in vivo, challenges with high fat diet and low-dose streptozotocin (STZ) were used to induce DM in C57BL/6J mice. AIS model was established by permanent middle cerebral artery occlusion (pMCAO) followed by intra-cerebroventricular injection of RvD2. RESULTS: Compared with macrophages of AIS patients without DM, the ratios of SPMs to leukotriene B4 (LTB4) were decreased in AIS patients with DM, accompanied by reduced expression of SPM synthesis enzyme, 15-lipoxygenase-1. Moreover, the levels of pro-inflammatory pathway markers were increased, and the macrophages were skewed to M1 polarization in AIS patients with DM. In mice, treatment with RvD2 ameliorated pMCAO-induced brain injury, neurological dysfunction, and inflammatory response. Furthermore, RvD2 rescued resolution of inflammation by promoting macrophage/microglia polarization to pro-resolving M2 phenotype ex vivo and in vivo. CONCLUSIONS: Our data demonstrate resolution of inflammation is impaired by DM in AIS patients, implicating a novel mechanism of un-resolved inflammation in DM-related AIS. Furthermore, RvD2 promotes inflammation resolution in macrophages/microglia and protects DM-related AIS, and may thus serve as a novel therapeutic target.


Assuntos
Diabetes Mellitus , AVC Isquêmico , Animais , Diabetes Mellitus/tratamento farmacológico , Ácidos Docosa-Hexaenoicos/metabolismo , Humanos , Infarto da Artéria Cerebral Média , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Leucócitos Mononucleares/metabolismo , Camundongos , Camundongos Endogâmicos C57BL
16.
J Oleo Sci ; 71(7): 1075-1084, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35691834

RESUMO

In recent years, secondary metabolites of plants have attracted researchers' interest as a substitute for synthetic insecticides with many advantages. Elsholtzia kachinensis is an annual herb with medicinal and edible value. In this study, the essential oil (EO) of the aerial part of E. kachinensis was extracted by hydrodistillation, and GC-MS analysed essential oil components. The results show that carvone and dehydroelsholtzia ketone are the main components of the essential oil, accounting for 32.298% and 31.540%, respectively. EO, carvone and dehydroelsholtzia ketone are used to determine the effects against stored-product insects Lasioderma serricorne, Tribolium castaneum, Sitophilus oryzae and Liposcelis bostrychophila. The essential oil showed the most vital contact and fumigation toxicity to L. serricorne, of which LD50 and LC50 values were 3.85 µg/adult and 7.74 mg/L air, respectively. S. oryzae did not show repellent activity, but the repellent rate of the other three species reached 90% under EO treatment at a concentration of 78.63 nL/cm2. Therefore, the essential oil of E. kachinensis has an insecticidal effect and has the potential to be developed as a new eco-friendly insecticide.


Assuntos
Besouros , Repelentes de Insetos , Inseticidas , Óleos Voláteis , Plantas Medicinais , Tribolium , Animais , Medicina Herbária , Repelentes de Insetos/análise , Inseticidas/análise , Inseticidas/toxicidade , Cetonas/análise , Óleos Voláteis/análise , Óleos Voláteis/toxicidade , Verduras
17.
Artif Intell Rev ; : 1-72, 2022 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-35693000

RESUMO

Microorganisms play a vital role in human life. Therefore, microorganism detection is of great significance to human beings. However, the traditional manual microscopic detection methods have the disadvantages of long detection cycle, low detection accuracy in large orders, and great difficulty in detecting uncommon microorganisms. Therefore, it is meaningful to apply computer image analysis technology to the field of microorganism detection. Computer image analysis can realize high-precision and high-efficiency detection of microorganisms. In this review, first,we analyse the existing microorganism detection methods in chronological order, from traditional image processing and traditional machine learning to deep learning methods. Then, we analyze and summarize these existing methods and introduce some potential methods, including visual transformers. In the end, the future development direction and challenges of microorganism detection are discussed. In general, we have summarized 142 related technical papers from 1985 to the present. This review will help researchers have a more comprehensive understanding of the development process, research status, and future trends in the field of microorganism detection and provide a reference for researchers in other fields.

18.
Ann Transl Med ; 10(10): 547, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35722410

RESUMO

Background: N6-methyladenosine (m6A) is found in almost all nuclear RNAs of eukaryotes, playing an important and diverse role in many biological processes. Nonetheless, the roles of m6A regulators in abdominal aortic aneurysm (AAA) unknown. Therefore, there is a pressing need to identify m6A RNA methylation regulators in the diagnosis of AAA, determination of individualized risk, discovery of therapeutic targets, and improve understanding of pathogenesis. Methods: The GSE98278 dataset were obtained from the Gene Expression Omnibus (GEO) database to perform differential analysis of m6A-related regulators between elective stable abdominal aortic aneurysms (eAAA) and abdominal aortic aneurysm ruptured (rAAA). The random forest model was used to screen candidate m6A regulators to predict the risk of rAAA. The single sample gene set enrichment analysis (ssGSEA) method was then used to evaluate the abundance of 23 immune cells in AAA. The m6A RNA Methylation Quantification Kit was used to measure the total m6A levels of AAA and normal abdominal aorta. The terminal deoxynucleotidyl transferase mediated dUTP Nick-End Labeling (TUNEL) apoptosis assay kit was used to detect the human aortic smooth muscle cells (HASMCs) apoptotic after RBM15 knockdown. Mechanically, RBM15 knockdown was found to reduce the expression of CASP3 in an m6A-dependent manner by Western blotting, RNA immunoprecipitation (RIP), and methylated RNA immunoprecipitation-quantitative polymerase chain reaction (MeRIP-RT-PCR). Results: RBM15, WTAP, ALKBH5, and IGFBP3 were highly expressed in rAAA. In contrast, RBM15B showed opposite results (P<0.05). The high m6A level in the rAAA compared with eAAA and normal abdominal aorta (P<0.05). The random forest model was used to screen 5 candidate m6A regulators to predict the risk of rAAA. Expression of the 5 m6A methylation regulators was validated in AAA samples (P<0.05). RBM15 knockdown inhibited the apoptosis of HASMCs. RBM15 knockdown reduced the expression of CASP3 in an m6A-dependent manner. A strong correlation between the five m6A methylation regulators and immune cell infiltration was identified. Conclusions: In summary, m6A regulators play nonnegligible roles in the occurrence of rAAA. Our investigation of m6A patterns may be able to guide future immunotherapy strategies for AAA.

19.
Nanoscale ; 14(26): 9431-9438, 2022 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-35730984

RESUMO

Reciprocating pressure-induced phase transition (RPPT) has been proposed as a new approach to synthesize nanostructured bulk materials with clean grain boundary interfaces for structures that undergo reversible pressure-induced phase transitions. The modulation effects on grain size under different cycle numbers of RPPT for InAs were investigated and the initial single-crystal bulk, with a dimensional size of about 30 µm, was transformed into a nanostructure with an average grain size of 7 nm by the utilization of the in situ high-pressure diamond anvil cell (DAC) technique. To verify the DAC findings, compact nanostructured bulk InAs with grain sizes ranging from 2-20 nm (average = 8 nm) and large dimensions (3.2 mm × 3.2 mm × 0.5 mm) was successfully synthesized from single-crystal InAs using a large volume press (LVP). The smaller work function (3.86 eV) and larger bandgap energy (2.64 eV) of the compact nanostructured bulk InAs phase compared to those of single-crystal InAs demonstrated that the nanostructure affected the macroscopic properties of InAs. The findings confirm the feasibility of synthesizing nanostructured bulk materials via RPPT.

20.
Zhongguo Zhong Yao Za Zhi ; 47(10): 2811-2818, 2022 May.
Artigo em Chinês | MEDLINE | ID: mdl-35718501

RESUMO

The present study evaluated the effect of Shenqi Jiangtang Granules(SJG) combined with western medicine on the adverse pregnancy outcomes in women with gestational diabetes mellitus(GDM). PubMed, Web of Science, CNKI, Wanfang, and VIP were searched for clinical randomized controlled trials(RCTs) of SJG combined with western medicine against GDM. The included RCTs were assessed for risks using the assessment criteria recommended by the Cochrane handbook for systematic reviews of interventions. Meta-analysis was performed using Stata 12.0 and RevMan 5.3. Nineteen RCTs were included, with 1 647 patients involved, including 824 cases treated with western medicine alone, and 823 cases treated with SJG combined with western medicine. The course of treatment ranged from 2 to 12 weeks. As revealed by Meta-analysis results, compared with western medicine treatment alone, SJG combined with western medicine could reduce the incidence of postpartum hemorrhage(OR=0.23, 95%CI[0.10, 0.53], P=0.000 6), gestational hypertension(OR=0.24, 95%CI[0.13, 0.45], P<0.000 01), polyhydramnios(OR=0.24, 95%CI[0.12, 0.45], P<0.000 1), premature rupture of membranes(OR=0.20, 95%CI[0.09, 0.45], P<0.000 1), cesarean section(OR=0.40, 95%CI[0.29, 0.55], P<0.000 01), macrosomia(OR=0.19, 95%CI[0.08, 0.47], P<0.000 3), neonatal asphyxia(OR=0.22, 95%CI[0.12, 0.40], P<0.000 01), premature delivery(OR=0.19, 95%CI[0.12, 0.30], P<0.000 01), proteinuria(OR=0.19, 95%CI[0.06, 0.58], P=0.004) and hypoglycemia(OR=0.28, 95%CI[0.16, 0.50], P<0.000 1). The funnel plots and Egger's test showed that except macrosomia, there was no significant publication bias in the results of other indicators. Therefore, as indicated by the findings, SJG combined with western medicine can reduce the incidence of adverse pregnancy outcomes in GDM patients. However, due to the uneven quality of the included trials, the clinical application of this protocol requires caution.


Assuntos
Diabetes Gestacional , Cesárea , Diabetes Gestacional/tratamento farmacológico , Medicamentos de Ervas Chinesas , Feminino , Macrossomia Fetal , Humanos , Recém-Nascido , Gravidez , Resultado da Gravidez , Revisões Sistemáticas como Assunto
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