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1.
J Environ Sci (China) ; 125: 332-339, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36375918

RESUMO

Mono-chlorinated products of cyclic volatile methylsiloxanes (cVMS), i.e., Monochlormet-hylheptamethylcyclotetrasiloxane [D3D(CH2Cl)], monochlormethylnonamethylcyclopenta-siloxane [D4D(CH2Cl)], and monochlormethylundemethylcyclohexasiloxane [D5D(CH2Cl)], were detected in water [

Assuntos
Campos de Petróleo e Gás , Poluentes Químicos da Água , Siloxanas/análise , Águas Residuárias , Água , Lagos , China , Monitoramento Ambiental , Sedimentos Geológicos
2.
J Colloid Interface Sci ; 629(Pt A): 522-533, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36088697

RESUMO

Photodynamic therapy (PDT) is proved to be a promising modality for clinical cancer treatment. However, it also suffers from a key obstacle in association with its oxygen-dependent nature which greatly limits its effective application against hypoxic tumors. Herein, on the basis of the unique property of calcium peroxide (CaO2), we propose an O2-self-supply strategy for the promotion of PDT by combining the in situ O2-generation characteristic of calcium peroxide with the photosensitive nature of porphyrin. A shell of ZIF-8 was synthesized surround the CaO2 core to prevent the CaO2 from premature decomposition and increased the loading of THPP efficiently. Depending on the in situ self-supply of O2, the photosensitizer was able to exhibit an enhanced PDT effect that significantly inhibit the growth of tumor. Moreover, the enrichment of free calcium ions derived from the decomposition of CaO2 under acidic tumor microenvironment also shows the unique ion-interference effect and contributes to the obvious inhibition against tumor growth. This work presents a synergistic strategy for the construction of a photodynamic promotion/ion-interference combined nano-platform which can also serve as an inspiration for the future design of effective nanocomposites in tumor treatment.


Assuntos
Nanopartículas , Neoplasias , Fotoquimioterapia , Porfirinas , Fármacos Fotossensibilizantes/farmacologia , Cálcio , Linhagem Celular Tumoral , Porfirinas/farmacologia , Oxigênio , Íons , Neoplasias/tratamento farmacológico
3.
Food Chem ; 403: 134264, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36182857

RESUMO

The aim of this study was to comprehensively investigate the effect of Holder pasteurization (HoP) compared with that of hydrostatic high-pressure (HHP) processing on human milk proteins, including milk fat globule membrane (MFGM) proteins, whey proteins and caseins. Milk fat globules in milk processed by high-pressure were similar to those in raw milk in terms of their size distribution and microstructure, while the globules in milk processed by HoP were aggregated. The protein profiles of milk subjected to HHP processing more closely resembled those of raw milk than HoP milk. Proteins in milk whey were less affected by HoP or HHP than MFGM and casein proteins. The findings indicated a better preservation of the protein profile for HHP compared to HoP of human milk.


Assuntos
Leite Humano , Proteômica , Humanos , Leite Humano/química , Pasteurização , Proteínas do Leite/química , Pressão Hidrostática , Caseínas/análise
4.
J Ethnopharmacol ; 301: 115852, 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36272494

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Qing-Xin-Jie-Yu Granule (QXJYG) is an integrated traditional Chinese medicine formula used to treat atherosclerotic (AS) cardiovascular diseases. A randomized controlled trial found that QXJYG reduced cardiovascular events and experiments also verified that QXJYG attenuated AS by remodeling the intestinal flora. AIM OF THE STUDY: To determine whether QXJYG would attenuate AS and plaque vulnerability by regulating ferroptosis in high-fat diet-induced atherosclerotic ApoE-/- mice and to investigate the effects of QXJYG on macrophage ferroptosis in RAS-selective lethal 3 (RSL3)-induced J744A.1 cells. METHODS: AS models in ApoE-/- mice and RSL3-induced ferroptosis in J744A.1 cells were established to measure the protective and anti-ferroptotic effects of QXJYG in vivo and in vitro. The glutathione peroxidase 4 (GPX4)/cystine glutamate reverse transporter (xCT) signal pathway was examined by immunohistochemistry and western blotting. RESULTS: QXJYG attenuated AS progression and plaque vulnerability. Characteristic morphological changes of ferroptosis in the QXJYG-treated animals were rare. Total iron was significantly lower in the QXJYG group than in the model group (P < 0.05); QXJYG suppressed the lipid peroxidation (LPO) levels (malondialdehyde), enhanced the antioxidant capacity (superoxide dismutase and glutathione), and reduced inflammatory factors (interleukin [IL]-6, IL-1ß, tumor necrosis factor-α) associated with ferroptosis. Expression of GPX4/xCT in aorta tissues was remarkably increased in the QXJYG group. QXJYG inhibited ferroptosis in J744A.1 macrophages disturbed using RSL3. The Fe2+, LPO, and reactive oxygen species levels were lower in the QXJYG group than in the RSL3 group (P < 0.05). The QXJYG group showed higher expression of the GPX4/xCT signal pathway. CONCLUSION: QXJYG inhibits ferroptosis in vulnerable AS plaques partially via the GPX4/xCT signaling pathway.


Assuntos
Ferroptose , Placa Aterosclerótica , Animais , Camundongos , Apolipoproteínas E , Placa Aterosclerótica/tratamento farmacológico , Transdução de Sinais , Sistemas de Transporte de Aminoácidos Acídicos/metabolismo
5.
Neural Regen Res ; 18(4): 819-824, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36204849

RESUMO

Epidural electrical stimulation is a new treatment method for spinal cord injury (SCI). Its efficacy and safety have previously been reported. Rehabilitation treatment after epidural electrical stimulation is important to ensure and improve the postoperative efficacy of epidural electrical stimulation in patients with SCI. Considering that electromyography (EMG)-induced rehabilitation treatment can accurately match the muscle contraction of patients with SCI, we designed a study protocol for a prospective, randomized controlled trial. In this trial, on the premise of adjusting the spinal cord electrical stimulator to obtain the maximum EMG signal of the target muscle, patients with SCI receiving epidural electrical stimulation will undergo EMG-induced rehabilitation treatment. Recovery of muscle strength of key muscles, quality of life, safety and therapeutic effects will be monitored. Twenty patients with SCI who are scheduled to undergo epidural electrical stimulation in Shanghai Ruijin Rehabilitation Hospital will be randomly divided into two groups with 10 patients per group. The control group will receive conventional rehabilitation treatment. The EMG-induced rehabilitation group will receive EMG-induced rehabilitation treatment of the target muscles of the upper and lower limbs based on conventional rehabilitation treatment. After rehabilitation treatment, follow up for all patients will occur at 2 weeks and 1, 3 and 6 months. The primary outcome measure of this trial will be evaluation of target muscle recovery using the Manual Muscle Testing grading scale. Secondary outcome measures will include modified Barthel Index scores, integrated EMG values, the visual analogue scale, Spinal Cord Independence Measure scores, and modified Ashworth scale scores. The safety indicator will be the incidence of adverse events. This trial will collect data regarding the therapeutic effects of EMG-induced rehabilitation in patients with SCI receiving epidural electrical stimulation for 6 months after rehabilitation treatment. Findings from this trial will help develop rehabilitation methods in patients with SCI after epidural electrical stimulation. This study protocol was approved by Ethics Committee of Shanghai Ruijin Rehabilitation Hospital (Approval No. RKIRB2022-12) on February 15, 2022 and was registered with Chinese Clinical Trial Registry (registration number: ChiCTR2200061674; date: June 30, 2022). Study protocol version: 1.0.

6.
Methods Mol Biol ; 2568: 147-163, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36227567

RESUMO

Small angle X-ray scattering (SAXS) has been widely applied as an enabling integrative technique for comprehensive analysis of the structure of biomacromolecules by multiple, complementary techniques in solution. SAXS in combination with computational modeling can be a powerful strategy bridging the secondary and 3D structural analysis of large RNAs, including the long noncoding RNAs (lncRNA). Here, we outline the major procedures and techniques in the combined use of SAXS and computational modeling for 3D structural characterization of a lncRNA, the subgenomic flaviviral RNA from Zika virus. lncRNA production and purification, RNA buffer and sample preparation for SAXS experiments, SAXS data collection and analysis, SAXS-aided RNA 3D structure prediction, and computational modeling are described.


Assuntos
RNA Longo não Codificante , Infecção por Zika virus , Zika virus , Simulação por Computador , Humanos , Modelos Moleculares , Conformação de Ácido Nucleico , Espalhamento a Baixo Ângulo , Difração de Raios X , Raios X , Zika virus/genética
7.
Spectrochim Acta A Mol Biomol Spectrosc ; 284: 121787, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36087404

RESUMO

Baijiu occupies a vital position in Chinese food and China's market. Strict evaluation of Baijiu is highly demanded. In this study, we constructed a novel fluorescent sensor array based on the single glutathione-protected gold nanoclusters (AuNCs) probe for the detection of organic acids and Baijiu. The fluorescence of AuNCs was simply modulated by three metal ions (Cu2+, Mn2+, and Ag+), and formed new complexes as sensing elements. These four sensing elements responded variously to nine organic acids, and further chemometric analysis results allowed for the classification and quantification of acids. Moreover, the sensor array successfully identified 21 Baijiu samples of different brands among 11 aroma types. It could also distinguish Baijiu of different qualities as well as pure Baijiu from its adulterations and showed high selectivity among multiple interfering drinks. The results demonstrated that the AuNCs-based sensor array has considerable potential for quality monitoring of Baijiu.


Assuntos
Ouro , Nanopartículas Metálicas , Corantes Fluorescentes , Glutationa , Íons , Espectrometria de Fluorescência/métodos
8.
J Hazard Mater ; 441: 129882, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36087532

RESUMO

Active oxygen species (AOS) play an essential role in modulating the activity of activated coke (AC) based samples. In this paper, AC was endowed with abundant AOS by modifying with (NH4)2S2O8 and MnOx-FeOx for Hg0 removal. (NH4)2S2O8 treatment induced abundant micropores and oxygen-containing functional groups, and thus provided more anchoring sites for the dispersion of MnOx-FeOx. The synergy of MnOx-FeOx and interaction between MnOx-FeOx and NAC support contributed to a larger surface area, highly-dispersed active components, stronger reducibility, and more metal ions with high valence of MnFe/NAC. The optimal MnFe/NAC exhibited superior Hg0 removal efficiency above 90% at 120∼180 â„ƒ, as well as excellent performance for simultaneous removal of Hg0 and NO, and 600 ppm SO2 and 8 vol.% H2O addition led to a slight deterioration. XPS and Hg-TPD revealed that mercury adsorbed on MnFe/NAC included phy-Hg, C=O-Hg, COO-Hg, and OL-HgO. Besides, the priority of AOS for Hg0 chemisorption was C=O > COO- > OL, and Hg2+ was also detected in the outlet. Moreover, the SO2-poisoning effect was ascribed to the sulfation of MnOx and the occupation of COO- and C=O, and FeOx incorporation enhanced the SO2-resistance through weakening SO2 adsorption on C=O and COO-. The motivation of O2 mainly contributed to the regeneration of AOS, especially OL. The excellent regeneration performance and stability further affirmed the application potential of MnFe/NAC for Hg0 capture from coal-fired flue gas.


Assuntos
Coque , Mercúrio , Sulfato de Amônio , Dióxido de Carbono , Catálise , Compostos Férricos , Ferro , Manganês , Óxidos/química , Oxigênio , Espécies Reativas de Oxigênio , Compostos de Enxofre/química , Dióxido de Enxofre
9.
Talanta ; 252: 123855, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36029683

RESUMO

Nucleus pH is closely linked to many diseases such as aging, heart disease, skeletal myopathies, cancer, Alzheimer's disease, etc. Nevertheless, fluorescent sensors that can directly monitor nucleus pH changes have not yet been reported. Here, we first reported a green emissive carbon dots (CDs) for nucleus pH detection in living cells. CDs can selectively target nucleus with high accumulation at nucleolus due to their high affinity towards RNA once entering cells by lipid raft mediated endocytosis. Without washing, CDs at 5 µg/mL was enough to lighten nucleus within 10 min with the fluorescence on ever after 24 h incubation, achieving fast, wash-free, and long-term nucleus/nucleolus imaging. Meanwhile, the luminescent intensity of CDs was reduced gradually when pH changed continuously from 1 to 12, showing a pH-responsive fluorescence property with two linear ranges of pH 2-7 and pH 7-12. With their nucleus-targeting ability and pH-dependent photoluminescent property, CDs was successfully leveraged for nucleus pH detection in A549 cells and for in vivo pH sensing in zebra fish. CDs present a promising and powerful fluorescent sensor for nucleus imaging and nucleus pH sensing in living cells on the way to understand nucleus-related biological events.


Assuntos
Carbono , Pontos Quânticos , Animais , Carbono/química , Pontos Quânticos/química , Corantes Fluorescentes/química , Microscopia de Fluorescência/métodos , Concentração de Íons de Hidrogênio , Espectrometria de Fluorescência
10.
Chemosphere ; 311(Pt 1): 136670, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36280116

RESUMO

Energy crisis and increasing rigorous management standards pose significant challenges for solid waste management worldwide. Several emerging diseases such as COVID-19 aggravated the already complex solid waste management crisis, especially sewage sludge and food waste streams, because of the increasingly large production year by year. As mature waste disposal technologies, landfills, incineration, composting, and some other methods are widespread for solid wastes management. This paper reviews recent advances in key sewage sludge disposal technologies. These include incineration, anaerobic digestion, and valuable products oriented-conversion. Food waste disposal technologies comprised of thermal treatment, fermentation, value-added product conversion, and composting have also been described. The hot topic and dominant research foci of each area are summarized, simultaneously compared with conventional technologies in terms of organic matter degradation or conversion performance, energy generation, and renewable resources production. Future perspectives of each technology that include issues not well understood and predicted challenges are discussed with a positive effect on the full-scale implementation of the discussed disposal methods.


Assuntos
COVID-19 , Eliminação de Resíduos , Humanos , Esgotos , Eliminação de Resíduos Líquidos/métodos , Alimentos , Resíduos Sólidos , Anaerobiose , Metano
11.
J Clin Transl Hepatol ; 11(1): 58-66, 2023 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-36406322

RESUMO

Background and Aims: Emerging evidence has demonstrated that abnormal body composition may potentiate the development of frailty, whereas little work focuses on the role of divergent adipose tissue. Therefore, we aimed to determine the potential contribution of adipose tissue distribution to multidimensional frailty in decompensated cirrhosis. Methods: We conducted a retrospective cohort study. Divergent adipose tissues were assessed by computed tomography-derived subcutaneous adipose tissue index (SATI), visceral adipose tissue index (VATI) and total adipose tissue index (TATI), respectively. Frailty was identified by our validated self-reported Frailty Index. Multiple binary logistic models incorporating different covariates were established to assess the relationship between adipose tissue distribution and frailty. Results: The study cohort comprised 245 cirrhotic patients with 45.3% being male. The median Frailty Index, body mass index (BMI) and model for end-stage liver disease (MELD) score were 0.11, 24.3 kg/m2 and 8.9 points, respectively. In both men and women, patients who were frail exhibited lower levels of SATI in comparison with nonfrail patients. SATI inversely correlated with Frailty Index in the entire cohort (rs=-0.1361, p=0.0332). Furthermore, SATI or TATI was independently associated with frail phenotype in several multiple logistic regression models adjusting for age, BMI, presence of ascites, sodium, Child-Pugh class or MELD score in isolation. Conclusions: In the context of decompensated cirrhosis, low SATI and concomitant TATI were associated with higher risk of being frail. These findings highlight the importance to further apply tissue-specific tools of body composition in place of crude metric like BMI.

12.
Rev. bras. med. esporte ; 28(6): 834-836, Nov.-Dec. 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1376725

RESUMO

ABSTRACT Introduction Kung fu exercise has a significant effect in treating lumbar disc herniation, effectively meeting the internal and external circulation of the human body functional system, the lumbar intervertebral disc movement function can be effectively repaired. Objective analyze and explore the effect of kung fu rehabilitation on symptoms of lumbar intervertebral disc herniation. Methods 85 patients with lumbar disc herniation were randomly divided into a control and a kung fu group. Results The comparison between the martial arts group before and after the experiment showed a very significant difference (p<0.05); there was no significant difference in the control group. The total effective rate in the kung fu group was 97.67%, and the relapse rate was 2.33%. The total effective rate of the control group was 90.48%, and the relapse rate was 21.43%. Conclusions Kung fu exercise is beneficial for the relaxation of waist muscles, relieves muscle tone, increases muscle strength, may improve clinical symptoms and signs of lumbar disc herniation. Evidence level II; Therapeutic Studies - Investigating the results.


RESUMO Introdução O exercício de kung fu tem um efeito significativo no tratamento da hérnia discal lombar, atendendo efetivamente à circulação interna e externa do sistema funcional corporal humano, a função de movimento do disco intervertebral lombar pode ser efetivamente reparada. Objetivo Analisar e explorar o efeito de reabilitação com kung fu nos sintomas da hérnia de disco intervertebral lombar. Métodos 85 pacientes com hérnia de disco lombar foram divididos aleatoriamente em um grupo de controle e um grupo de kung fu. Resultados A comparação entre o grupo de artes marciais antes e depois do experimento mostrou uma diferença muito significativa (p<0,05); não houve diferença significativa no grupo controle. A taxa efetiva total no grupo de kung fu foi de 97,67%, e a taxa de recidiva foi de 2,33%. A taxa efetiva total do grupo de controle foi de 90,48%, e a taxa de recidiva foi de 21,43%. Conclusões O exercício de kung fu é benéfico para o relaxamento dos músculos da cintura, alivia o tônus muscular, aumenta a força muscular; pode melhorar os sintomas clínicos e os sinais de hérnia de disco lombar. Nível de evidência II; Estudos terapêuticos - Investigação de resultados.


RESUMEN Introducción el ejercicio de kung fu tiene un efecto significativo en el tratamiento de la hernia discal lumbar, atendiendo eficazmente a la circulación interna y externa del sistema funcional del cuerpo humano, la función de movimiento del disco intervertebral lumbar puede repararse eficazmente. Objetivo Analizar y explorar el efecto de la rehabilitación con kung fu en los síntomas de la hernia discal intervertebral lumbar. Métodos 85 pacientes con hernia discal lumbar fueron divididos aleatoriamente en un grupo de control y un grupo de kung fu. Resultados La comparación entre el grupo de artes marciales antes y después del experimento mostró una diferencia altamente significativa (p<0,05); no hubo diferencia significativa en el grupo de control. La tasa de efectividad total en el grupo de kung fu fue del 97,67%, y la tasa de recaída fue del 2,33%. La tasa efectiva total del grupo de control fue del 90,48%, y la tasa de recidiva del 21,43%. Conclusiones El ejercicio de kung fu es beneficioso para la relajación de los músculos de la cintura, alivia el tono muscular, aumenta la fuerza muscular; puede mejorar los síntomas y signos clínicos de la hernia discal lumbar. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

13.
RSC Adv ; 12(43): 28021-28033, 2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36320228

RESUMO

Red emissive carbon dots (R-CDs) have received great attention in biological fields due to their deep tissue penetrability, great bioimaging capability, low interference from auto-fluorescence, and potential for optoelectronic applications. Herein, excitation-independent, highly acid-sensitive R-CDs were successfully obtained via one-step microwave treatment of o-phenylenediamine (o-PD) and phosphoric acid and carefully purified by column chromatography. The relationship between the fluorescence emission and surface groups of the R-CDs was studied in detail using XPS, NMR, and fluorescence spectroscopy, and the different mechanisms of action of the R-CDs and acid in H2O and ethanol were determined. The excellent anti-interference ability and biocompatibility of the R-CDs were confirmed, and the probes were successfully used for imaging A549 and Escherichia coli (E. coli) cells in extreme acidity. Finally, based on their relatively high quantum yield and long wavelength emission, the application potential of the R-CDs in the fabrication of red light-emitting diodes (LEDs) was investigated.

14.
RSC Med Chem ; 13(9): 1064-1076, 2022 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-36324492

RESUMO

A series of new ß-carboline derivatives containing an imidazolium moiety were designed and synthesized via the reaction of ß-carboline-1-carboxaldehydes, acetyl chloride, primary amine, and formaldehyde. The antitumor activity of the synthesized compounds was examined against lung carcinoma (A549), gastric carcinoma (BGC-823), murine colon carcinoma (CT-26), liver carcinoma (Bel-7402) and breast carcinoma (MCF-7) cells. The results indicated that most compounds exhibited significant antiproliferative activity, in some cases greater than that of cisplatin, and compound 3z was found to be the most potent antiproliferative agent against A549, BGC823, CT-26, Bel-7402 and MCF-7 cell lines with an IC50 value of 2.7 ± 0.4, 2.7 ± 0.6, 2.4 ± 0.2, 3.2 ± 0.2, and 5.6 ± 0.3 µM, respectively. Combined with favorable in vitro potency, the antitumor efficacies of the selected compounds in mice were also evaluated. Compound 3z exhibited potent antitumor activity with a tumor inhibition rate of 48.6% in sarcoma 180 models. Preliminary investigations on the mechanisms of action revealed that compound 3z could dramatically inhibit EA.hy926 cell tube formation in a dose-dependent manner. Further investigation of the preliminary mechanism of action demonstrated that compound 3z had obvious angiogenesis inhibitory effects in the chicken chorioallantoic membrane (CAM) assay. The results of the docking study showed a good fitting of the new compounds 3o and 3z to the active site of VEGFR-2 with a docking score energy of -11.31 kcal per mole and -11.26 kcal per mole, respectively.

15.
Front Med ; 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36331793

RESUMO

Preeclampsia (PE) is characterized by placenta-mediated pregnancy complication. The only effective treatment for PE is the delivery of the placenta. However, this treatment may cause preterm birth and neonatal death. Therefore, preventing PE is needed. The mechanism of PE involves abnormal placentation, which leads to the release of anti-angiogenic and inflammatory mediators into maternal circulation. These mediators contribute to systemic vascular dysfunction, inflammatory responses, and excessive thrombin generation. Microparticles (MPs) are reportedly involved in PE by promoting the thromboinflammatory response. This study describes a strategy to prevent PE by reducing MP release using the recombinant protein, diannexin. Results showed that the patients with PE had elevated MP number and procoagulant activity and increased NLRP3 inflammasome activation. Additionally, diannexin remarkably reduced the release of MPs from activated cells by binding to phosphatidylserine exposed on the surface of activated cells. Moreover, in vivo results showed that diannexin could prevent PE-like symptoms by decreasing MPs and NLRP3 inflammasome activation in pregnant mice. Furthermore, diannexin effectively inhibited trophoblast cell activation and NLRP3 inflammasome activation in vitro. These findings suggested that diannexin inhibited MP release and might be an effective therapeutic strategy for preventing PE.

17.
Front Microbiol ; 13: 931431, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36329847

RESUMO

Objective: Rheumatoid arthritis (RA) is a chronic inflammatory joint disease, which is associated with progressive disability, systemic complications, and early death. But its etiology and pathogenesis are not fully understood. We aimed to investigate the alterations in plasma metabolite profiles, gut bacteria, and fungi and their role of them in the pathogenesis of RA. Methods: Metabolomics profiling of plasma from 363 participants including RA (n = 244), systemic lupus erythematosus (SLE, n = 50), and healthy control (HC, n = 69) were performed using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The differentially expressed metabolites were selected among groups and used to explore important metabolic pathways. Gut microbial diversity analysis was performed by 16S rRNA sequencing and ITS sequencing (RA = 195, HC = 269), and the specific microbial floras were identified afterward. The diagnosis models were established based on significant differential metabolites and microbial floras, respectively. Results: There were 63 differential metabolites discovered between RA and HC groups, mainly significantly enriched in the arginine and proline metabolism, glycine, serine, and threonine metabolism, and glycerophospholipid metabolism between RA and HC groups. The core differential metabolites included L-arginine, creatine, D-proline, ornithine, choline, betaine, L-threonine, LysoPC (18:0), phosphorylcholine, and glycerophosphocholine. The L-arginine and phosphorylcholine were increased in the RA group. The AUC of the predictive model was 0.992, based on the combination of the 10 differential metabolites. Compared with the SLE group, 23 metabolites increased and 61 metabolites decreased in the RA group. However, no significant metabolic pathways were enriched between RA and SLE groups. On the genus level, a total of 117 differential bacteria genera and 531 differential fungal genera were identified between RA and HC groups. The results indicated that three bacteria genera (Eubacterium_hallii_group, Escherichia-Shigella, Streptococcus) and two fungal genera (Candida and Debaryomyces) significantly increased in RA patients. The AUC was 0.80 based on a combination of six differential bacterial genera and the AUC was 0.812 based on a combination of seven differential fungal genera. Functional predictive analysis displayed that differential bacterial and differential fungus both were associated with KEGG pathways involving superpathway of L-serine and glycine biosynthesis I, arginine, ornithine, and proline interconversion. Conclusion: The plasma metabolism profile and gut microbe profile changed markedly in RA. The glycine, serine, and threonine metabolism and arginine and proline metabolism played an important role in RA.

18.
Quant Imaging Med Surg ; 12(11): 5198-5208, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36330179

RESUMO

Background: For complicated Stanford type B aortic dissection (TBAD), thoracic endovascular aortic repair (TEVAR) is the recommended treatment; however, the type of renal artery that should be repaired remains controversial. The study aimed to investigate the changes in the renal artery and renal volume in complicated TBAD after TEVAR and the predictors of renal atrophy. Methods: The cohort study retrospectively enrolled patients with acute and subacute complicated TBAD who underwent aortic computed tomography angiography (CTA) 1 month before as well as 1 week and half a year after TEVAR from January 2010 to May 2017. According to the source of blood supply shown in preoperative CT, the renal artery was classified in 3 ways: type 1, supplied by the aortic true lumen; type 2, supplied by the aortic false lumen; or type 3, supplied by both the true and false lumen. Results: A total of 91 patients (81 men and 10 women) with an average age of 48.12±10.35 years were enrolled. Renal arteries were classified as type 1 (n=91), type 2 (n=35), and type 3 (n=56). There was no difference in the distribution of the 3 types on the left and right sides (type 1 vs. type 2 vs. type 3: 52:39 vs. 15:20 vs. 24:32; P=0.152). After TEVAR, type 3 was more likely to have spontaneous healing than type 2 (16.1% vs. 2.9%; P=0.049). There was no significant difference in the preoperative volume of kidneys of the 3 types (type 1 vs. type 2 vs. type 3: 198.23±38.68 vs. 197.37±41.77 vs. 195.10±36.11 mL; P=0.893). The postoperative volume of types 2 and 3 was smaller than that of type 1 (type 1 vs. type 2 vs. type 3: 190.09±43.25 vs. 165.15±52.63 vs. 170.70±45.28 mL; P=0.006). The renal volume was reduced in all 3 types of renal artery, especially in type 2 (the change of renal volume for type 1 vs. type 2 vs. type 3: -8.14±29.31 vs. -32.22±41.59 vs. -24.41±38.44 mL; P=0.001). The relative change of renal volume for type 1 vs. type 2 vs. type 3: (-3.64±15.69)% vs. (-16.00±21.29)% vs. (-11.97±18.22)%; P=0.001). During the median follow-up of 668 days, 7 patients (7.7%) belonging to types 2 and 3 developed renal atrophy. False lumen thrombosis in the abdominal aorta and/or the renal artery was the predictor of renal atrophy [hazard ratio (HR) =17.757; P=0.008]. Conclusions: Patients with type 2 or 3 renal artery and false lumen thrombosis in the abdominal aorta and/or renal artery should be monitored closely and actively intervened to prevent renal atrophy.

19.
Bioinspir Biomim ; 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36322980

RESUMO

A bee tongue is coated in dynamic hairs that gradually unfold to entrain the viscid nectar, during which hairs inevitably deflect caused by the fluid drag. The hair deflection induced decline in nectar capture rate may be a coupled elastoviscous problem, which remains poorly understood. Here we employed the geometric beam theory coupling with the effective viscous force to derive a dynamic model for a rotary tongue hair deflection in a viscous fluid. Considering deflection of the tongue hair, we rationalized the nectar capture rate by taking the Bombus as a model system. When the nectar concentration increases from 20% to 70%, the nectar capture rate declines by 87%, indicating that hair erection is more severely impeded in thicker nectar. Based on this model, we predicted an optimal hair length by which the bee can reach the maximum nectar capture rate. This work may provide a new theoretical framework for quantifying viscous liquid transport by hairy surfaces and shed light on design methodologies for fluid transport devices using hairy beds.

20.
Chem Biol Interact ; 368: 110242, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36326519

RESUMO

Based on the scaffold hybridization strategy, twenty-four indole-guanidines were designed and synthesized. Subsequently, anti-proliferative activity against various cancer cells indicated that most of these hybrids exhibited moderate to high anti-proliferative activity, especially for human hepatoma cell lines. Selectivity investigation showed that these hybrids showed the best selectivity for SMMC-7721 subtype in human hepatoma cells. Particularly, (E)-3-((2-(N-pentylcarbamimidoyl)hydrazono)methyl)-1H-indol-5-yl 4-methylbenzoate (19) and (E)-3-((2-(N-pentylcarbamimidoyl)hydrazono)methyl)-1H-indol-5-yl 4-methoxybenzoate (22) exhibited potent inhibition against SMMC-7721 cells with IC50 values of 0.057 µM and 0.042 µM, respectively, far outperforming that of Sorafenib. Meanwhile, hybrids 19 and 22 exhibited no significant cytotoxicity against normal cells such as HEK293 cells and HEK293T cells. Moreover, further investigations indicated that hybrid 22 effectively induced apoptosis, arrested the cell cycle at S phase, and selectively down regulated expression of p-STAT3, JAK2 and BRAF in SMMC-7721 cells in a dose-dependent manner. Molecular docking indicated that hybrid 22 exhibited high affinity with STAT3 and BRAF. In summary, hybrid 22 was developed as a potential and effective anti-hepatoma candidate, which was worthy of further investigation.

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