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1.
Food Chem ; 338: 127947, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32947120

RESUMO

Hydrogen peroxide (H2O2) is a reactive oxygen species (ROS) that mediates essential signaling in vivo but may cause irreversible tissue damage under dysregulated or acute exposure conditions. Beverages containing redox-active compounds might produce H2O2 during shelf storage and potentially be consumed. Concentrations of H2O2 in selected 'functional' (including energy, E, n = 28), 'non-functional' flavored, (S, n = 6) and mineral water (W, n = 6) drinks were measured under ambient (i.e., produced in situ) and 'potentiated' conditions (i.e., H2O2 production enhanced by addition of a reducing agent, to simulate availability of reducible substrates in vivo). Under air-saturated conditions, mean H2O2 contents were: 15.60 ± 15.84; 1.39 ± 2.06 and 0.30 ± 0.21 µM in E, S and W drinks, respectively. Under air-saturated, potentiated conditions, mean rates of H2O2 production were 21.7 ± 33.3, 0.98 ± 2.84, and -0.38 ± 1.18 µM/h for E, S and W drinks, respectively. Using multivariate statistics, the ingredient significantly associated with H2O2 production in combination with other ingredients was found to be ascorbic acid.

2.
Food Chem ; 334: 127593, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32711276

RESUMO

ß-Glucosidases play an important role in food industry. Oenococcus oeni are typical lactic acid bacteria that initiate malolactic fermentation of wines. 35 ß-glucosidases from O. oeni were selected and their conserved domains and evolutionary relationships were further explored in this study. The homology analysis results indicated that 35 ß-glucosidases were basically derived from GH1 and GH3 family. A novel ß-glucosidase was successfully expressed and characterized. The recombinant protein, referred to as BGL0224, consisted of a total 480 amino acids with an apparent molecular weight of 55.15 kDa and was classified as GH1 family. It achieved the highest activity at pH 5.0 and 50 °C. The activity and stability were significantly increased when 12% ethanol was supplemented to the enzyme. Using p-NPG as substrate, the Km, Vmax and Kcat of BGL0224 were 0.34 mM, 382.81 U/mg and 351.88 s-1, respectively. In all, BGL0224 has good application prospects in food industry.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32512131

RESUMO

Autism spectrum disorder (ASD) is accompanied with widespread impairment in social-emotional functioning. Classification of ASD using sensitive morphological features derived from structural magnetic resonance imaging (MRI) of the brain may help us to better understand ASD-related mechanisms and improve related automatic diagnosis. Previous studies using T1 MRI scans in large heterogeneous ABIDE dataset with typical development (TD) controls reported poor classification accuracies (around 60%). This may because they only considered surface-based morphometry (SBM) as scalar estimates (such as cortical thickness and surface area) and ignored the neighboring intrinsic geometry information among features. In recent years, the shape-related SBM achieves great success in discovering the disease burden and progression of other brain diseases. However, when focusing on local geometry information, its high dimensionality requires careful treatment in its application to machine learning. To address the above challenges, we propose a novel pipeline for ASD classification, which mainly includes the generation of surface-based features, patch-based surface sparse coding and dictionary learning, Max-pooling and ensemble classifiers based on adaptive optimizers. The proposed pipeline may leverage the sensitivity of brain surface morphometry statistics and the efficiency of sparse coding and Max-pooling. By introducing only the surface features of bilateral hippocampus that derived from 364 male subjects with ASD and 381 age-matched TD males, this pipeline outperformed five recent MRI-based ASD classification studies with >80% accuracy in discriminating individuals with ASD from TD controls. Our results suggest shape-related SBM features may further boost the classification performance of MRI between ASD and TD.

4.
Artigo em Inglês | MEDLINE | ID: mdl-33038300

RESUMO

PURPOSE: To review the demographic data, presenting symptoms, location, radiographic findings, treatment, and prognosis of pediatric jaw lesions in children treated at a single academic institution. PATIENTS AND METHODS: A retrospective medical record review was undertaken of patients younger than 18 years who presented to the Massachusetts General Hospital for Children between 2006 and 2018 with a primary jaw lesion. RESULTS: About 164 patients were identified. The most common lesions were giant cell tumors (n = 25), odontogenic keratocysts (n = 24), simple bone cysts (n = 19), odontomas (n = 17), fibrous dysplasia (n = 11), and dentigerous cysts (n = 11). Fifty-one patients (30.7%) were asymptomatic. About 94% were referred by their dentist, outside oral and maxillofacial surgeon or orthodontist. Most common presenting symptoms were swelling (66.9%), pain (32.5%), tooth mobility (17.5%), and neurosensory change (6.6%). Mandibular location was most common (72.3%). Radiographically, most were well-circumscribed radiolucencies with mean size of 2.9 cm (range, 0.7 to 15.6). Treatment varied from excisional biopsy to wide composite resection. Mean follow-up time was 38 months (range, 1 to 204). Recurrence was found in 21%. CONCLUSIONS: Pediatric jaw lesions are often asymptomatic and discovered incidentally by dental practitioners on routine examination. Clinical features (age, gender, location, and radiographic appearance) can help narrow the differential and expedite treatment. It is important that clinicians involved in the care of children be familiar with the wide differential diagnosis and management considerations of primary jaw lesions.

5.
Dalton Trans ; 49(39): 13797-13804, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33000838

RESUMO

Three novel phosphorescent iridium(iii) complexes with thieno[2,3-d]pyridazine derivatives as cyclometalating chelates were successfully synthesized. These complexes exhibited intense green or yellow phosphorescence emission with short lifetimes of 1.71-1.91 µs and very high quantum yields of over 85% in PMMA films. Even in air-equilibrated CH2Cl2, their quantum yields could be up to 36%. They also showed good thermal stability with Td > 338 °C. The electronic structure information of these complexes was discussed by density functional theory. The tris-cyclometalated complex Ir1 was an excellent yellow phosphorescent dopant for OLED applications, and the maximum CE and PE based on it were 58.5 cd A-1 and 45.9 lm W-1, respectively, with the maximum EQE of 18.2% which was ca. 1.4 times more efficient than that of PO-01. These results indicate that these new complexes have potential applications as efficient phosphors in OLEDs.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33044123

RESUMO

Near road measurements in Rochester, NY with a Portable Air Quality Monitoring System indicate a significant plume control of PM2.5 black carbon (BC) concentrations. This study evaluates the performance of two portable air quality enclosures deployed at collocated research sites to determine their accuracy and usefulness in field deployments, and specifically in pollution plume analysis. One system deployed collocated sensors for measurement of particulate matter mass concentration (Thermo pDR 1500 against Tapered Element Oscillating Microbalance (TEOM) measurement) and the second system deployed sensors for measurement of black carbon (Magee AE33 aethalometer and Brechtel Tricolor Absorption Photometer) in ambient and near road locations in Rochester, New York, respectively. While the optical PM2.5 sensors tended to be biased in their determination of concentration by ~15%, they followed changes and trends in concentration very well. The black carbon sensors in the portable systems agreed very well with each other and with the collocated sensor. As a case study to determine the contribution from statistically significant short-lived excursions of pollutant concentration, Morlet wavelet analysis was performed on data from the portable system sensors. Black carbon was found to be strongly influenced by plume behavior with significant plume excursions representing just over 12% of all data points and contributing on average 1 µg/m3 of black carbon above ambient concentrations. Implications Statement This paper first evaluates two air pollutant monitoring enclosures with wide applicability including near road detection of pollutants. Then, we present a novel method to designate isolate statistically significant excursions in air pollution concentration which can be used to determine the impact of pollutant plumes as observed in PM and black carbon behavior near road.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33044868

RESUMO

Females are protected against the development of Angiotensin II (Ang II)-induced hypertension compared with males, but the mechanisms are not completely elucidated. In the present study, we hypothesized that the effect of Ang II on macula densa nitric oxide (NO) synthase ß (NOS1ß)-mediated tubuloglomerular feedback (TGF) mechanism is different between male and female, thereby contributing to the sexual dimorphism of Ang II-induced hypertension. We used microperfusion, micropuncture, clearance of FITC-inulin, and radio telemetry to examine the sex differences in the changes of macula densa NOS1ß expression and activity, TGF response, natriuresis, and blood pressure (BP) after 2-weeks Ang II infusion in wild-type and macula densa-specific NOS1 knockout mice. In wild-type mice, Ang II induced higher expression of macula densa NOS1ß, greater NO generation by the macula densa, as well as lower TGF response in vitro and in vivo in females than in males; the increases of GFR, urine flow rate and sodium excretion in response to an acute volume expansion were significantly greater and the BP responses to Ang II were significantly less in females than in males. In contrast, these sex differences in the effects of Ang II on TGF, natriuretic response and BP were largely diminished in knockout mice. Additionally, tissue culture of human kidney biopsies (renal cortex) with Ang II resulted in a greater increase in NOS1ß expression in females than in males. In conclusion, macula densa NOS1ß-mediated TGF is a novel and important mechanism for the sex differences in Angiotensin II-Induced hypertension.

8.
Eur J Pediatr ; 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33048238

RESUMO

This study reports on vocal cord paralysis caused by esophageal button battery (BB) ingestion in children. Medical records of children with vocal cord paralysis and esophageal BB ingestion treated at a tertiary referral institute between January 2016 and March 2020 were reviewed. Five patients aged 9-58 months were identified; three were male. Each patient had accidentally swallowed a 20-mm-diameter lithium battery (3 CR2032 type and 2 CR2025 type). One battery was removed within 4 h after ingestion, and three batteries were removed within 12 h. Removal of the battery was achieved with rigid esophagoscopy in four patients and direct laryngoscopy in one patient. Four patients had bilateral, and one had unilateral vocal cord paralysis. Three patients underwent tracheotomy; two were tracheotomy-dependent until follow-up, while the third patient died of multiple organ dysfunction syndrome a month after surgery. The two cases without tracheotomy remain under close follow-up.Conclusion: Accidentally ingested button batteries should be removed promptly to avoid severe complications. Respiratory support and neurotrophic treatment in the early stage of vocal cord paralysis may be beneficial for recovery of vocal cord movement. What is Known: • Button battery ingestion in children is extremely harmful, especially when the diameter of the button battery exceeds 20 mm. • Esophageal button battery impaction can cause serious complications such as esophageal perforation, mediastinal infection, tracheoesophageal fistula, vocal cord paralysis, and life-threatening bleeding. What is New: • Vocal cord paralysis in children with button battery ingestion plays an early warning role for identifying the severity of the disease. • Early neurotrophic drugs and glucocorticoid therapy may be helpful for the recovery of vocal cord movement, thereby avoiding tracheotomy.

9.
J Hazard Mater ; 404(Pt B): 124044, 2020 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-33059153

RESUMO

Bauxite mine tailings can be used as the reserve resources of aluminium and lithium. In this study, a less energy consumption treatment method for extracting aluminium and lithium from bauxite mine tailings has been proposed, which used mixed acid to leach aluminium and lithium from tailings directly, avoided roasting for reducing energy consumption, and obtained effective results.The minerals in the tailings are kaolinite, diaspore, boehmite, anatase and illite, among others. The minerals have fine dissemination sizes and low liberation. Under the leaching conditions of Al and Li of an acid concentration of 60%, a liquid-solid ratio of 4 mL/g, a reaction temperature of 100 °C and a reaction time of 3 h, the highest leaching rates of Al and Li are 88.64% and 96.35%, respectively. In the leaching process, phosphoric acid reacts with Al and Li in the strong acidic environment provided by sulphuric acid to produce heteropoly acids or heteropoly acid salts that dissolve in the solution.

10.
Medicine (Baltimore) ; 99(42): e22603, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33080694

RESUMO

The derived neutrophil-lymphocyte ration (dNLR) is a systemic inflammatory marker.The present study focusing on the prognostic value of pre-treatment dNLR in patients of early stage non-small cell lung cancer (NSCLC).From 2012 to 2016, patients with newly diagnosed early stage NSCLC were investigated. Only these who treated with stereotactic ablative radiotherapy (SABR) were enrolled in this study. dNLR was calculated from complete blood count prior to SABR. The optimal cut-off value of dNLR was determined by receiver operating curve. Kaplan-Meier curves and Cox proportional models were used to analyze the impact of pre-treatment dNLR on disease free survival (DFS) and overall survival (OS).There were 69 patients eligible for analysis, the median follow-up period was 30.9 months. Calculated by receiver operating characteristic curves, the optimal cut off value of dNLR was 1.99. Kaplan-Meier curves demonstrated that a decreased dNLR was correlated with favorable DFS and OS. In univariate analysis, high dNLR was associated with decreased survival; moreover, multivariate analysis revealed that a decreased dNLR was an independent significant favorable prognostic factor for both DFS and OS.An elevated pre-treatment dNLR may be an independent prognostic biomarker for DFS and OS in patients with early stage NSCLC that are eligible for SABR. dNLR is a reliable, inexpensive, simple, and readily available tool for risk-stratification and should be considered in daily clinical practice.

11.
Sci Rep ; 10(1): 18197, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33082397

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

12.
World J Clin Cases ; 8(19): 4572-4578, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33083420

RESUMO

BACKGROUND: Squamous cell carcinoma antigen (SCCA) is regarded as a specific indicator of epithelial malignancies and is widely used in the diagnosis of squamous cell carcinoma (SCC). However, the expression of SCCA in gastric adenocarcinoma has not been studied in detail. CASE SUMMARY: A 52-year-old man was admitted to our hospital for a 2.5 cm × 2.5 cm ulcer at the antrum-body junction with dull pain and fullness in the upper abdomen for 2 mo. His pre-surgery serological testing results showed 0.51 ng/mL SCCA (reference interval, < 1.5 ng/mL) and 9.9 ng/mL carcinoembryonic antigen (reference range, < 4.7 ng/mL). He underwent radical distal gastrectomy and Roux-en Y anastomosis and was diagnosed with poorly differentiated mucinous adenocarcinoma (Lauren classification: Diffuse) by pathological examination of the resected lesion. Immunohistochemistry showed that SCCA was highly expressed in the cytoplasm of cancer cells. After surgery, the patient received an S-1 adjuvant chemotherapy regimen for six cycles containing tegafur, gimeracil, and oteracil potassium. He showed no sign of recurrence or metastasis within 24-mo follow-up. CONCLUSION: This is a frontal report of SCCA overexpression in poorly differentiated adenocarcinoma of the stomach.

13.
Artif Cells Nanomed Biotechnol ; 48(1): 1272-1281, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33084450

RESUMO

Volume resuscitation is an important early treatment for haemorrhagic shock. Haemoglobin-based oxygen carrier (HBOC) can expand the volume and provide oxygen for tissues. Vascular leakage is common complication in the process of haemorrhagic shock and resuscitation. The aim of this study was to observe the effects of HBOC (a bovine-derived, cross-linked tetramer haemoglobin oxygen-carrying solution, 0.5 g/L) on vascular leakage in rats after haemorrhagic shock. A haemorrhagic shock rat model and hypoxic vascular endothelial cells (VECs) were used. The role of intercellular junctions and endothelial glycocalyx in the protective effects of HBOC and the relationship with mitochondrial function were analysed. After haemorrhagic shock, the pulmonary vascular permeability to FITC-BSA, Evans Blue was increased, endothelial glycocalyx was destroyed and the expression of intercellular junction proteins was decreased. After haemorrhagic shock, a small volume of HBOC solution (6 ml/kg) protected pulmonary vascular permeability, increased structural thickness of endothelial glycocalyx, the levels of its components and increased expression levels of the intercellular junction proteins ZO-1, VE-cadherin and occludin. Moreover, HBOC significantly increased oxygen delivery and consumption in rats, improved VEC mitochondrial function and structure. In conclusion, HBOC mitigates endothelial leakage by protecting endothelial glycocalyx and intercellular junctions through improving mitochondrial function and tissue oxygen delivery.

14.
Chemosphere ; 264(Pt 1): 128410, 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-33002803

RESUMO

Community-intrinsic properties affect the composition and function of a microbial community. Understanding the microbial community-intrinsic properties in drinking water distribution systems (DWDS) could help to select disinfection strategies and aid in the prevention of waterborne infectious diseases. In this study, we investigated the formation of multi-species biofilms in six groups, each consisting of four or five mixed bacterial strains isolated from a simulated DWDS, at different incubation times (24, 48, and 72 h). We then evaluated the chlorine resistance of the 72-h multi-species biofilms in the presence of 0.3, 0.6, 1, 2, 4, and 10 mg/L residual chlorine. Microbacterium laevaniformans inhibited the formation of multi-species biofilms, Sphingomonas sp., Acinetobacter sp. and A. deluvii had the effect of promoting their growth, and B. cereus has little effect on the growth of multi-species biofilms. However, these inhibition and promotion effects were weak and inadequate to completely control the growth of multi-species biofilms. All multi-species produced strong biofilms after 72 h incubation, which could be due to microbial community-intrinsic properties. Community-intrinsic properties could maintain high EPS production and cell-to-cell connections in multi-species biofilms, and could affect the formation of multi-species biofilms. The chlorine resistance of multi-species biofilms was significantly improved by B. cereus, but significantly reduced by M. laevaniformans. These results indicated that the microbial community-intrinsic properties were influenced by the environment. At a relatively low disinfectant concentration (<2 mg/L residual chlorine), the community-intrinsic properties were maintained; however, when the disinfectant concentration was increased to 2-4 mg/L residual chlorine, the community-intrinsic properties weakened, and significantly affected the resistance of the microbial communities to the disinfectant. With further increases in concentration, to >4 mg/L residual chlorine, no significant difference was observed in the disinfectant resistance of the microbial community.

15.
J Ethnopharmacol ; 266: 113426, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-33007392

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: In traditional Chinese medicine, the fruit of Schisandra chinensis (Turcz.) Baill (SC) is used to treat various nervous system diseases, such as dysphoria, anxiety, insomnia and many dreams. It is worthy to be noted that wine processed Schisandra chinensis (WSC) has been applied in clinic for thousands of years. AIM OF STUDY: This study aimed to investigate the possible mechanism and related metabolism of SC and WSC ameliorating anxiety behavior through modulating gut microbiota. MATERIALS AND METHODS: The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used for the quality control of chemical components in SC and WSC. Chronic unpredictable stress procedure (CUSP)-induced anxiety rats were administrated with SC and WSC via gavage for five weeks. An untargeted UPLC/LTQ-Orbitrap MS metabolomic analysis of plasma was conducted to understand the effects of long-term intake of WSC and SC extracts on anxious rats. 16S rRNA microbial sequencing technology was applied to investigate gut microbiota structure. Expression of GPR81, TNF-α, S1PR2 as well as molecules in cAMP pathway was assayed by immunohistochemistry staining, RT-qPCR, or Western blot, respectively. RESULTS: 12 compounds were identified using UPLC-QTOF-MS technology, all of which are lignans. Results demonstrate that the amounts of 6-O-Benzoylgomisin O, Schisandrin, Gomisin D, Schizandrin A, Gomisin T, Schizandrin B, Schisandrin C were higher in wine-processed samples than in raw samples. Furthermore, both SC and WSC significantly ameliorated anxiety- and depression-like behavior and lipid metabolism dysfunction and attenuated hippocampal neuritis in anxiety rats. After WSC treatment, the structure and composition of gut microbiota in anxiety rats changed significantly, and gut microbiota derivatives lactate level was significantly lower in the plasma and feces. WSC treatment help restore gut microbial ecosystem dysbiosis and reverse the changes in Lachnospiraceae, Lactobacillus, Alloprevotella, and Bacteroidales in anxiety rat. In addition, the expression of liver GPR81 was decreased, and the molecules in cAMP pathway were increased in SC and WSC-treated anxiety rat. CONCLUSION: Raw and wine processed Schisandra chinensis treatment improved anxiety- and depression-like behavior through modulating gut microbiota derivatives in association with GPR81 receptor-mediated lipid metabolism pathway. And WSC has more exhibition than SC.

16.
Accid Anal Prev ; 148: 105777, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33011425

RESUMO

BACKGROUND: Although numerous efforts have been devoted to exploring the effects of area-wide factors on the frequency of pedestrian crashes in neighborhoods over the past two decades, existing studies have largely failed to provide a full picture of the factors that contribute to the incidence of zonal pedestrian crashes, due to the unavailability of reliable exposure data and use of less sound analytical methods. METHODS: Based on a crowdsourced dataset in Hong Kong, we first proposed a procedure to extract pedestrian trajectories from travel-diary survey data. We then aggregated these data to 209 neighborhoods and developed a Bayesian spatially varying coefficients model to investigate the spatially non-stationary relationships between the number of pedestrian-motor vehicle (PMV) crashes and related risk factors. To dissect the role of pedestrian exposure, the estimated coefficients of models with population, walking trips, walking time, and walking distance as the measure of pedestrian exposure were presented and compared. RESULTS: Our results indicated substantial inconsistencies in the effects of several risk factors between the models of population and activity-based exposure measures. The model using walking trips as the measure of pedestrian exposure had the best goodness-of-fit. We also provided new insights that in addition to the unstructured variability, heterogeneity in the effects of explanatory variables on the frequency of PMV crashes could also arise from the spatially correlated effects. After adjusting for vehicle volume and pedestrian activity, road density, intersection density, bus stop density, and the number of parking lots were found to be positively associated with PMV crash frequency, whereas the percentage of motorways and median monthly income had negative associations with the risk of PMV crashes. CONCLUSIONS: The use of population or population density as a surrogate for pedestrian exposure when modeling the frequency of zonal pedestrian crashes is expected to produce biased estimations and invalid inferences. Spatial heterogeneity should also not be negligible when modeling pedestrian crashes involving contiguous spatial units.

17.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 3869-3872, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018845

RESUMO

The objective of this study is to develop an experimental protocol to define the range of modulation for different sensory modalities elicited by transcutaneous electrical nerve stimulation (TENS) in amputees with evoked tactile sensation (ETS). Modulation ranges of sensory modalities, such as vibration, buzz, tingling etc., are essential for designing a coding strategy for incoming sensory information from prosthetic hands for each amputee. Here, the modulation ranges of different modalities in pulse width at fixed stimulus frequencies were evaluated. Four healthy subjects and three transradial amputee subjects were recruited to participate in this preliminary test. A single skin site in healthy subjects and five finger areas of the projected finger map (PFM) on the stump skin in amputee subjects were stimulated for evaluation. Then, a finger identification test was conducted to show the feasibility of this sensory coding strategy in one amputee subject. The sensitivity of different sensory modalities was also measured to quantify the minimum pulse width change that subject could distinguish. Results showed that vibration and buzz sensations had wider modulation ranges in pulse width for both healthy and amputee subjects at 20Hz and 50Hz of stimulation, respectively. The average accuracy of finger identification was 91.66% in the amputee subject. The minimum pulse width changes distinguishable for both vibration and buzz sensation were below 20 (us). Results indicated that the protocol of evaluating pulse width modulation ranges for different sensory modalities was effective, and the coding strategy could provide accurate finger-specific sensory information for amputees with ETS.Clinical Relevance- This protocol establishes a guideline for customizing the coding strategy of evoked tactile sensory feedback for each amputee.

18.
Aging (Albany NY) ; 12(19): 19352-19364, 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33049714

RESUMO

CircRNAs have been shown to be associated with gastric cancer tumorigenesis. But little was known about the role of circPDZD8 in gastric cancer. CircPDZD8 was up-regulated in gastric cancer tissues and cells, Kaplan-Meier survival analysis indicated that gastric patients had a poor overall survival when circPDZD8 levels were high. CircPDZD8 knockdown could hinder proliferation and migration of gastric cancer cells. MiR-197-5p, which was down-regulated in gastric cancer, was shown to be a target of circPDZD8 and was inversely correlated with circPDZD8 expression. CHD9, as a target gene of miR-197-5p, was negatively regulated by miR-197-5p and positively correlated with circPDZD8 expression. Importantly, circPDZD8 could up-regulate CHD9 expression by sponging miR-197-5p, and modulate cell progression by regulation of the miR-197-5p/CHD9 axis in gastric cancer. CircPDZD8 knockdown repressed the progression of gastric cancer cells by sponging miR-197-5p and down-regulating CHD9.

19.
J Cosmet Dermatol ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33025709

RESUMO

BACKGROUND/OBJECTIVE: Recent researches had reported that microRNAs (miRNAs) played a role in skin photoaging. Our previous study found that long noncoding RNA (lncRNA) expression was changed in the UVA-irradiated skin fibroblasts, but the regulating network of noncoding RNA in UV-induced skin changes has not been elucidated well. Here, we investigated the interactions of miRNA-lncRNA-mRNAs in skin photoaging mechanisms. METHODS: Human dermal fibroblasts (HDFs) were irradiated with UVA at 10 J/cm2 once a day lasting for 14 days. miRNA expression profiles were detected by high-throughput sequencing. miRNAs changed significantly were identified by qRT-PCR. Functional annotation analysis and pathway enrichment were carried out using Gene Ontology and KEGG, and predicted miRNA-lncRNA-mRNA interactions were performed via bioinformatic analysis. RESULTS: 34 differentially expressed miRNAs (>1.5-fold changes, P < .05) after UVA irradiation were identified to interact with distinct lncRNAs. miRNA-lncRNA-mRNA network prediction and regulatory role analysis showed that the gene expression of cellular process, cell part, and binding was mainly coordinated in UVA-irradiated fibroblasts. miRNA-lncRNA-mRNA-signal transduction pathway analysis showed that TNF signaling pathway, thyroid hormone signaling pathway, and lysosome were mainly affected after UVA irradiation. CONCLUSION: miRNA-lncRNA-mRNA network played a critical part in skin photoaging. Our research provided novel insights into the repeated UVA-induced skin damage in noncoding RNA regulatory field and might help to further understand the delicate interplay of gene regulation at the noncoding RNA level in photoaged skin and UV-induced skin cancers in future researching and provide novel insights into the repeated UVA-damaging pathology and potential targets for preventing human skin photoaging.

20.
Cell Host Microbe ; 28(4): 602-613.e7, 2020 10 07.
Artigo em Inglês | MEDLINE | ID: mdl-33031770

RESUMO

In 2014, an outbreak of avian A/H10N7 influenza virus occurred among seals along North-European coastal waters, significantly impacting seal populations. Here, we examine the cross-species transmission and mammalian adaptation of this influenza A virus, revealing changes in the hemagglutinin surface protein that increase stability and receptor binding. The seal A/H10N7 virus was aerosol or respiratory droplet transmissible between ferrets. Compared with avian H10 hemagglutinin, seal H10 hemagglutinin showed stronger binding to the human-type sialic acid receptor, with preferential binding to α2,6-linked sialic acids on long extended branches. In X-ray structures, changes in the 220-loop of the receptor-binding pocket caused similar interactions with human receptor as seen for pandemic strains. Two substitutions made seal H10 hemagglutinin more stable than avian H10 hemagglutinin and similar to human hemagglutinin. Consequently, identification of avian-origin influenza viruses across mammals appears critical to detect influenza A viruses posing a major threat to humans and other mammals.

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