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1.
Mol Cancer ; 21(1): 37, 2022 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-35130920

RESUMO

PURPOSE: The overall response of cisplatin-based chemotherapy in bladder urothelial carcinoma (BUC) remains unsatisfactory due to the complex pathological subtypes, genomic difference, and drug resistance. The genes that associated with cisplatin resistance remain unclear. Herein, we aimed to identify the cisplatin resistance associated genes in BUC. EXPERIMENTAL DESIGN: The cytotoxicity of cisplatin was evaluated in six bladder cancer cell lines to compare their responses to cisplatin. The T24 cancer cells exhibited the lowest sensitivity to cisplatin and was therefore selected to explore the mechanisms of drug resistance. We performed genome-wide CRISPR screening in T24 cancer cells in vitro, and identified that the gene heterogeneous nuclear ribonucleoprotein U (HNRNPU) was the top candidate gene related to cisplatin resistance. Epigenetic and transcriptional profiles of HNRNPU-depleted cells after cisplatin treatment were analyzed to investigate the relationship between HNRNPU and cisplatin resistance. In vivo experiments were also performed to demonstrate the function of HNRNPU depletion in cisplatin sensitivity. RESULTS: Significant correlation was found between HNRNPU expression level and sensitivity to cisplatin in bladder cancer cell lines. In the high HNRNPU expressing T24 cancer cells, knockout of HNRNPU inhibited cell proliferation, invasion, and migration. In addition, loss of HNRNPU promoted apoptosis and S-phase arrest in the T24 cells treated with cisplatin. Data from The Cancer Genome Atlas (TCGA) demonstrated that HNRNPU expression was significantly higher in tumor tissues than in normal tissues. High HNRNPU level was negatively correlated with patient survival. Transcriptomic profiling analysis showed that knockout of HNRNPU enhanced cisplatin sensitivity by regulating DNA damage repair genes. Furthermore, it was found that HNRNPU regulates chemosensitivity by affecting the expression of neurofibromin 1 (NF1). CONCLUSIONS: Our study demonstrated that HNRNPU expression is associated with cisplatin sensitivity in bladder urothelial carcinoma cells. Inhibition of HNRNPU could be a potential therapy for cisplatin-resistant bladder cancer.


Assuntos
Antineoplásicos , Carcinoma de Células de Transição , Neoplasias da Bexiga Urinária , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Apoptose , Carcinoma de Células de Transição/tratamento farmacológico , Linhagem Celular Tumoral , Proliferação de Células , Cisplatino/farmacologia , Cisplatino/uso terapêutico , Resistencia a Medicamentos Antineoplásicos/genética , Ribonucleoproteínas Nucleares Heterogêneas Grupo U , Humanos , Neoplasias da Bexiga Urinária/tratamento farmacológico , Neoplasias da Bexiga Urinária/genética , Neoplasias da Bexiga Urinária/patologia
2.
Rev. bras. med. esporte ; 28(6): 734-737, Nov.-Dec. 2022. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1376722

RESUMO

ABSTRACT Introduction Progressive strength training can help athletes improve their strength levels quickly and safely. By controlling the frequency and amplitude of vibrations, muscles can produce unconscious and voluntary contractions that help prevent diseases such as osteoporosis. Objective To explore the change in sports performance caused by progressive upper limb muscle strength training in table tennis players. Methods 12 table tennis players with a minimum experience of 5 years were randomly divided into experimental and control groups. The control performed traditional strength training methods while the experimental group used the progressive directed strength training method developed by the research team for nine weeks. Tests were performed on ISOMED 2000 equipment at the conditions of 60°/s and 450°/s. Results Circumference, muscle strength, and relative upper limb strength increased. In addition, a difference in the rate of the maximum elbow flexion moment of the athletes in the experimental group in the 60°/s test conditions was observed. At 450°/s, the maximum flexion and extension elbow moment showed a significant difference (P<0.05). Conclusion The progressive strength training method significantly affects the athletes' maximum elbow flexion moment and maximum elbow extension moment than traditional strength training methods. Muscle strength progress was noted mainly in the elbow and wrist joints, with less intensity in the shoulders. Evidence Level II; Therapeutic Studies - Investigating the results.


RESUMO Introdução O treinamento progressivo da força pode ajudar os atletas a melhorar seus níveis de força de maneira rápida e segura. Controlando a frequência e a amplitude das vibrações, os músculos podem produzir contrações inconscientes e voluntárias que ajudam a prevenir doenças como a osteoporose. Objetivo Explorar a alteração no desempenho esportivo ocasionado pelo treinamento progressivo da força muscular nos membros superiores dos jogadores de tênis de mesa. Métodos 12 jogadores de tênis de mesa com experiência mínima de 5 anos foram aleatoriamente divididos em grupos experimental e controle. O controle efetuou métodos tradicionais de treinamento de força enquanto o grupo experimental utilizou o método progressivo de treinamento de força dirigida, desenvolvido pela equipe de pesquisa, por nove semanas. Os testes foram realizados no equipamento ISOMED 2000 nas condições de 60°/s e 450°/s. Resultados A circunferência, força muscular e a relativa força dos membros superiores aumentaram. Houve diferença na taxa do momento de flexão máxima do cotovelo dos atletas do grupo experimental nas condições de teste de 60°/s. A 450°/s, o momento máximo de flexão do cotovelo e o momento máximo de extensão do cotovelo também mostraram uma diferença significativa (P<0,05). Conclusão O método de treinamento progressivo de força afeta significativamente o momento máximo de flexão do cotovelo e o momento máximo de extensão do cotovelo dos atletas do que os métodos tradicionais de treinamento de força. O progresso da força muscular foi notado principalmente nas articulações do cotovelo e pulso, com menor intensidade nos ombros. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.


RESUMEN Introducción El entrenamiento de fuerza progresivo puede ayudar a los atletas a mejorar sus niveles de fuerza de forma rápida y segura. Al controlar la frecuencia y la amplitud de las vibraciones, los músculos pueden producir contracciones inconscientes y voluntarias que ayudan a prevenir enfermedades como la osteoporosis. Objetivo Explorar el cambio en el rendimiento deportivo provocado por el entrenamiento progresivo de la fuerza muscular en las extremidades superiores de los jugadores de tenis de mesa. Métodos 12 jugadores de tenis de mesa con una experiencia mínima de 5 años fueron divididos aleatoriamente en grupos experimental y de control. El grupo de control realizó métodos de entrenamiento de fuerza tradicionales, mientras que el grupo experimental utilizó el método de entrenamiento de fuerza dirigido progresivo desarrollado por el equipo de investigación durante nueve semanas. Las pruebas se realizaron en el equipo ISOMED 2000 en las condiciones de 60°/s y 450°/s. Resultados La circunferencia, la fuerza muscular y la fuerza relativa de los miembros superiores aumentaron. Hubo una diferencia en la velocidad del momento de flexión máxima del codo de los atletas del grupo experimental en las condiciones de prueba de 60°/s. A 450°/s, el momento máximo de flexión del codo y el momento máximo de extensión del codo también mostraron una diferencia significativa (P<0,05). Conclusión El método de entrenamiento de fuerza progresivo afecta significativamente al momento de flexión máxima del codo de los atletas y al momento de extensión máxima del codo que los métodos tradicionales de entrenamiento de fuerza. El progreso de la fuerza muscular se observó principalmente en las articulaciones del codo y la muñeca, con menor intensidad en los hombros. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

3.
J Clin Neurosci ; 104: 1-9, 2022 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-35931000

RESUMO

The current prediction models for the clinical outcome of acute ischaemic stroke (AIS) remain insufficient for individualized patient management strategies. We aimed to investigate machine learning (ML) performance in the clinical outcome prediction of AIS in anterior circulation and evaluate the clinical outcome by combining the quantitative evaluation indicators of perfusion features and basic clinical information. Four ML classifiers, support vector machine (SVM), naive Bayes (NB), logistic regression (LR), and random forest (RF) were trained on a cohort of 389 adult patients (training cohort [70 %]; external validation cohort [30 %]) from the Acute Stroke Center Registry of Huashan Hospital. Model performance was compared by a range of learning metrics. Most imaging parameters were strongly correlated with the outcome (range, 0.57 to 0.81), and the correlation between relative cerebral blood flow (rCBF) < 30 % and clinical outcome was the strongest (ρ = 0.81). As the reference parameters increased, the performance of the four models was greatly improved [SVM (AUC: from 0.79 to 0.99, F1-score: from 0.61 to 0.90), RF (AUC: from 0.88 to 0.98, F1-score: from 0.71 to 0.96), LR (AUC: from 0.80 to 0.97, F1-score: from 0.64 to 0.95), and NB (AUC: from 0.74 to 0.97, F1-score: from 0.66 to 0.92)]. The ensemble classifier model with all parameters had the highest F1-score (0.97). All the ML models, jointly considering the basic clinical information and quantitative evaluation indicators of computed tomography perfusion (CTP), showed good performance in the prediction of clinical outcome of AIS in anterior circulation.

4.
Cancer Epidemiol ; 80: 102236, 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35939867

RESUMO

BACKGROUND & AIMS: Initial screening for high-risk population of gastric cancer (GC) is needed in rural areas of large-population countries. This study aims to explore the feasibility of applying noninvasive ultrasonography as an initial screening strategy to improve the early diagnosis and prevention of GC. METHODS: Oral contrast-enhanced trans-abdominal ultrasonography (OCTU) was initially applied to screen around 15,000 residents from 24 different rural villages of Changxing Island in Shanghai, China, facilitating the identification of high-risk population for further endoscopy examination. RESULTS: 176 subjects (1.18 %) were initially identified with gastric diseases using OCTU while 14,787 ones (98.93 %) were normal with negative results. 145 out of 176 individuals (82.39 %) took further endoscopy examination, and 16 were diagnosed with GC with biopsy examination, with 9 of them at the early stage. We followed up with the Center for Disease Control and Prevention, and identified another 6 GC cases occurred within one year among OCTU-negative population, serving as an adjustment factor for sensitivity analysis. As a result, with a total of 22 GC cases included in this cohort, the positive predictive rate, the negative predictive value, sensitivity, and specificity were 9.09%, 99.96 %, 75.5 %, and 98.93 %, respectively. CONCLUSIONS: OCTU is feasible, non-invasive, low-cost, and widely acceptable in rural area, thus we proposed that OCTU is practicable to serve as a supplementary screening method to improve the early detection of GC in rural area of China and other developing countries with large population.

5.
Water Res ; 222: 118899, 2022 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-35940152

RESUMO

The ubiquitous microplastics (MPs) in water environment play an important role in the dissemination of antibiotic resistance genes (ARGs) due to their exchange between floating MPs and receiving waters. However, whether the ARG exchange is persistent or transient and what are the differences in ARG exchange between conventional plastics and biodegradable plastics are the two key issues to be addressed. In this study, biodegradable PBAT and non-biodegradable PET MPs were chosen to explore the MP-water ARG exchange after the MPs floated to the receiving waters. The results demonstrated that the active exchange of ARGs between MPs and receiving waters occurred, which, however, were transient for most of ARGs. The relative abundance of ARGs both on the MPs and in the waters rapidly decreased to the initial or lower levels within 4 weeks. Approximately 25-50% (ARG subtype number ratio) of studied ARG subtypes were introduced into the receiving waters by MPs, and 35-65% of studied ARG subtypes went through fluctuation in terms of abundance on MPs and in the receiving water. ARGs tended to converge between MPs and the receiving waters with time. Furthermore, the ARG exchange between MPs and waters facilitated horizontal gene transfer (HGT). IntI1 and tnpA05 played the crucial roles in HGT, which was indicated by their correlated change with most ARGs; in contrast, tnpA04 showed the obvious lagging responses. The biodegradable MP of PBAT generally accumulated higher levels of most ARGs including multidrug resistant genes than the non-biodegradable MP of PET. The transient exchange of most ARGs between MPs and water implies that the on-off hitchhiking of ARGs on MPs in aquatic environment may not exert significant influence on ARG transmission. However, compared with the conventional plastics, the biodegradable MPs might pose much higher ARG dissemination risks due to the higher enrichment of ARGs particularly with people's ever-increasingly usage. Enough attention must be paid to this emerging issue.

6.
Stem Cell Res Ther ; 13(1): 407, 2022 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-35941707

RESUMO

BACKGROUND: Large area skin trauma has always been a great challenge for both patients and clinicians. Exosomes originating from human adipose-derived mesenchymal stem cells (hADSCs) have been a novel promising cell-free treatment in cutaneous damage repair. Nevertheless, the low retention rate of exosomes post-transplantation in vivo remains a significant challenge in clinical applications. Herein, we purposed to explore the potential clinical application roles of hADSCs-Exos encapsulated in functional PF-127 hydrogel in wound healing. METHODS: hADSCs-Exos were isolated from human hADSCs by ultracentrifugation. An injectable, biocompatible, and thermo-sensitive hydrogel Pluronic F-127 hydrogel was employed to encapsulate allogeneic hADSCs-Exos, and this complex was topically applied to a full-thickness cutaneous wound in mice. On different days post-transplantation, the mice were sacrificed, and the skin tissue was excised for histological and immunohistochemical analysis. RESULTS: Compared with hADSCs-Exos or PF-127 only, PF-127/hADSCs-Exos complexes enhanced skin wound healing, promoted re-epithelialization, increased expression of Ki67, α-SMA, and CD31, facilitated collagen synthesis (Collagen I, Collagen III), up-regulated expression of skin barrier proteins (KRT1, AQP3), and reduced inflammation (IL-6, TNF-α, CD68, CD206). By using PF-127/hADSCs-Exos complexes, hADSCs-Exos can be administrated at lower doses frequency while maintaining the same therapeutic effects. CONCLUSION: Administration of hADSCs-Exos in PF-127 improves the efficiency of exosome delivery, maintains the bioactivity of hADSCs-Exos, and optimizes the performance of hADSCs-Exos. Thus, this biomaterial-based exosome will be a promising treatment approach for the cutaneous rejuvenation of skin wounds.


Assuntos
Exossomos , Células-Tronco Mesenquimais , Animais , Colágeno/metabolismo , Exossomos/metabolismo , Humanos , Hidrogéis/farmacologia , Células-Tronco Mesenquimais/metabolismo , Camundongos , Poloxâmero/metabolismo , Poloxâmero/farmacologia , Cicatrização
7.
Int Ophthalmol ; 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35947254

RESUMO

PURPOSE: The objective of this article was to examine the potential effect of juvenile idiopathic arthritis-associated uveitis (JIAU) on the risk of major depressive and anxiety disorders through Mendelian randomization (MR) study. METHODS: Genetic instrumental variables from the largest available genome-wide association study for JIAU, major depressive disorder, and anxiety disorder were applied. A set of complementary MR approaches including inverse-variance weighted (IVW) were carried out to verify the estimate association and assess horizontal pleiotropy. RESULTS: Our results indicated that genetically driven JIAU did not causally produce changes in major depressive or anxiety disorders (IVW: OR = 1.001, 95% CI = 0.997-1.006, P = 0.581; IVW: OR = 1.006, 95% CI = 0.980-1.033, P = 0.649, respectively). In addition, the risk of JIAU could not be influenced by genetically predicted major depressive or anxiety disorders (IVW: OR = 1.132, 95% CI = 0.914-1.404, P = 0.256; IVW: OR = 1.019, 95% CI = 0.548-1.896, P = 0.953, respectively). Besides, several sensitivity analyses indicated that our MR results were robust and no horizontal pleiotropy was observed (P > 0.05). CONCLUSIONS: Our MR study does not reveal sufficient evidence to support the causal association of JIAU with the development of major depressive or anxiety disorders in both directions. Further large studies are warranted to validate the undetermined relationship between JIAU and the risk of major depressive or anxiety disorders.

8.
J HIV AIDS Soc Serv ; 21(1): 1-15, 2022 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-35937313

RESUMO

Introduction: Among persons with HIV (PWH), homelessness is associated with poorer health. From 2009-2014, national HIV prevention goals included a reduction in homelessness among PWH. We sought to examine social ecological factors associated with homelessness among PWH at a sub-national level during that period. Methods: National data were used to identify Delaware as the only jurisdiction where homelessness among PWH declined from 2009-2014. We analyzed population-level indicators and conducted telephone interviews with 6 key stakeholders to further examine this trend. Results: Overall homelessness, household poverty, and median housing price were associated with homelessness among PWH in Delaware. Key stakeholders indicated that centralized intake processes improved screening, referral, and linkages of clients to housing units. Discussion: In addition to social and economic factors, collaborative program strategies may improve housing outcomes for PWH. Monitoring trends at sub-national levels can help identify successful approaches as well as needed services or policy change.

9.
Artigo em Inglês | MEDLINE | ID: mdl-35939453

RESUMO

Protein-protein interactions (PPIs) play an essential role for most of biological processes in cells. Many computational algorithms have thus been proposed to predict PPIs. However, most of them heavily rest on the biological information of proteins while ignoring the latent structural features of proteins presented in a PPI network. In this paper, we propose an efficient network-based prediction algorithm, namely PPISB, based on a mixed membership stochastic blockmodel. By simulating the generative process of a PPI network, PPISB is able to capture the latent community structures. The inference procedure adopted by PPISB further optimizes the membership distributions of proteins over different complexes. After that, a distance measure is designed to compute the similarity between two proteins in terms of their likelihoods of being in the same complex, thus verifying whether they interact with each other or not. To evaluate the performance of PPISB, a series of extensive experiments have been conducted with five PPI networks collected from different species and the results demonstrate that PPISB has a promising performance when applied to predict PPIs in terms of several evaluation metrics. Hence, we reason that PPISB is preferred over state-of-the-art network-based prediction algorithms especially for predicting potential PPIs.

10.
Inorg Chem ; 2022 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-35943140

RESUMO

A spinel compound FeAl2S4 was successfully synthesized under high-pressure and high-temperature conditions and was systematically characterized via the structural, magnetic, and specific heat measurements. It crystallizes into a cubic structure with the space group Fd3̅m (no. 227) and the lattice constant a = 10.0207(2) Å. A Fe/Al site inversion is found; that is, the molecular formula can be rewritten as (Fe1-xAlx)(Al2-xFex)S4, and the inversion parameter x is about 0.22. Magnetic susceptibility measurements indicate that FeAl2S4 undergoes a spin glass behavior, which is confirmed by ac susceptibility and specific heat measurements. The freezing temperature Tf ∼ 10.5 K and Weiss temperature Tθ ∼ -107.4 K lead to a high frustration parameter f = |Tθ/Tf| of about 10, which suggests that FeAl2S4 is a high-frustration magnet. Our results indicate that high pressure can help stabilize the spinel structure with small R̅σ and the cation inversion plays an important role in the formation of the spin glass state.

11.
Front Mol Neurosci ; 15: 960460, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35909446

RESUMO

Screening serum biomarkers for acute and subacute pain is important for precise pain management. This study aimed to examine serum levels of angiogenic factors in patients with acute and subacute pain as potential biomarkers. Serum samples were collected from 12 healthy controls, 20 patients with postherpetic neuralgia (PHN), 4 with low back pain (LBP), and 1 with trigeminal neuralgia (TN). Pain intensity in these patients was evaluated using the visual analog scale (VAS). The serum concentrations of 11 angiogenic biomarkers were examined by Milliplex Map Human Angiogenesis Magnetic Bead Panel 2. The pain assessment from VAS showed that all patients showed moderate and severe pain. Among 11 angiogenic factors, osteopontin (OPN), thrombospondin-2 (TSP-2), soluble platelet endothelial cell adhesion molecule-1 (sPECAM-1), soluble urokinase-type plasminogen activator receptor (suPAR), and soluble epidermal growth factor receptors (sErbB2) were up-regulated and soluble interleukin-6 receptor α (sIL-6Rα) were down-regulated in patients with pain compared to the healthy participants (all P-values were < 0.005). Moreover, a linear regression model showed that the serum OPN concentration was correlated with pain intensity in patients with PHN (P = 0.03). There was no significant difference between the serum concentration of soluble epidermal growth factor receptors, sErbB3, soluble AXL, tenascin, and soluble neuropilin-1 in patients with acute and subacute pain and that of healthy controls. The results of this study provided new valuable insights into our understanding of angiogenic factors that may contribute to as mechanistic biomarkers of pain, and reveal the pathophysiological mechanism of pain. Clinical Trial Registration: www.chictr.org.cn, identifier ChiCTR2200061775.

12.
Front Surg ; 9: 956346, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35910479

RESUMO

Background: Currently, the extent of 4sb and 12a lymph node dissection is not clear and is based on the personal understanding of the surgeon. It may result in damage to the splenic artery and portal vein, leading to surgical complications. Therefore, this study aims to explore the scope of 4sb and 12a lymph node dissection in cT2-4 lower third gastric cancer. Methods: This is an ongoing prospective cohort trial. The total sample size required for the trial (March 2022 to February 2025) is approximately 524 patients. The participants are divided into the experimental (4sb first branch and 12a anterior lymphadenectomy) or control groups (traditional 4sb and 12a lymphadenectomy). Electronic data capture systems will be used to collect demographic, laboratory test, auxiliary examination, operation, postoperative condition, postoperative pathology, and follow-up data. The primary outcome is the 12a lymph node metastatic rate. Secondary outcomes include the pathology (consisting of the 4sb lymph node metastatic rate, the number of 4sb lymph nodes dissected, the number of 12a lymph nodes dissected and tumor pathological staging), a safety evaluation index (consisting of complications and mortality ≤30 days after surgery), an efficacy evaluation (consisting of operation data and postoperative recovery status), and follow-up data (consisting of 3-year or 5-year disease-free survival and overall survival). Discussion: By exploring the scope of 4sb and 12a lymph node dissection on the premise of ensuring radical cure of the tumor, the operation is simplified, the operation time is shortened, the damage of important blood vessels is reduced, the intraoperative and postoperative complications are reduced, and the patient recovers as soon as possible. Our study is a prospective exploration of the pathology, safety, efficacy, and prognosis of the new and traditional methods of 4sb and 12a lymph node dissection. Trial registration: Chinese Clinical Trial Registry, ChiCTR2200057698 (registration date: March 15, 2022).

13.
Environ Sci Technol ; 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35914180

RESUMO

Hypertensive disorders in pregnancy (HDP) are a leading cause of maternal mortality and adverse birth outcomes. Fine particulate matter (PM2.5) has been linked to HDP risk; however, limited studies have explored the relationships between specific chemical constituents of PM2.5 and HDP risk. Based on maternal data from the China Labor and Delivery Survey (CLDS), this study included a total of 67,659 participants from 95 participant hospitals in 25 provinces of China between March 1, 2015, and December 31, 2016. Maternal exposure to total PM2.5 mass and six main components during pregestation and pregnancy were estimated using the Combined Geoscience-Statistical Method. Multilevel logistic regression models were applied to quantify the associations, controlling for sociodemographic characteristics. We found that an interquartile range (IQR) increase in PM2.5 exposure during the second trimester was associated with a 14% increase in HDP risk (95% CI: 2%, 29%). We observed that black carbon (BC) and SO42- had larger or comparable estimates of the effect than total PM2.5 mass. The association estimates were greater in the gestational hypertension group than in the group of pre-eclampsia and eclampsia. Our findings suggest that PM2.5 exposure and specific chemical components (particularly BC and SO42-) were associated with an increased HDP risk in China.

14.
Inorg Chem ; 2022 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-35916903

RESUMO

Low-dimensional coordination polymers such as one-dimensional chains often exhibit gated guest sorption accompanying structural transition at a temperature (TG), which is associated with an external pressure of the guest (PG) characteristic to the material and guest used. This phenomenon can be evaluated using the Clausius-Clapeyron relationship with the equation d(ln PG)/d(1/TG) = ΔHG/R, where ΔHG and R are the transition enthalpy and gas constant, respectively. In this study, gated CO2 adsorption behavior was investigated in a one-dimensional chain based on a benzoate-bridged paddlewheel diruthenium(II,II) complex with a phenazine (phz) linker, [Ru2(p-MeOPhCO2)4(phz)] (1; p-MeOPhCO2- = p-anisate). Surprisingly, 1 underwent gate opening (GO)/closing (GC) at a much higher TG, e.g., 385 K for GC, under PCO2 = 100 kPa than those previously reported for such chain compounds, which usually appeared in the temperature range of 200-270 K. The transition entropy ΔSG in each system plays a key role in shifting TG; 1 results in a much smaller |ΔSG| in the series. Only 1 produced a CO2-accessible two-dimensional topological pore in its CO2-adsorbed phase 1⊃CO2, whereas the others reported previously produced one-dimensional or discrete topological pores for CO2 accommodation, strongly reflecting the degree of freedom of CO2 molecules in pores, which is related to ΔSG.

15.
J Food Biochem ; : e14372, 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35929524

RESUMO

Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota. C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks. The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL). In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury. The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed. The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks. In summary, this study provided evidence for the therapeutic effects of probiotics on alcoholic liver injury by regulating intestinal flora.

16.
PLoS One ; 17(8): e0266734, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35913982

RESUMO

In order to further improve the accuracy of the video-based behavior recognition method, an effective behavior recognition method in the video session using convolutional neural network is proposed. Specifically, by adding the target detection phase before the behavior recognition algorithm, the body region in the video can be accurately extracted to reduce the interference of redundant and unnecessary background noises, and at the same time, the inappropriate images can be replaced, which has reached the role of balance background trade-off, and finally, the neural network can learn the human behavior information with emphasis. By adding fragmentation and stochastic sampling, the long-time time-domain modeling of the whole video session can be established, so that the model can obtain video-level expression ability. Finally, the improved loss function is used for behavior recognition to solve the problem of classification difficulty and possible sample imbalance. In addition, we conducted the hyperparametric experiment, the ablation experiment and the contrast experiment on different open source and benchmark datasets. Compared with other commonly used behavior recognition algorithms, the experimental results verify the effectiveness of the proposed method. In addition, the related deep learning-based methods used in behavior recognition are reviewed at the beginning of this paper, and the challenges in behavior recognition and future research directions are prospected at the end of this paper, which will undoubtedly play a double role in the work of later researchers.


Assuntos
Algoritmos , Redes Neurais de Computação , Benchmarking , Humanos
17.
BMC Infect Dis ; 22(1): 663, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35915398

RESUMO

BACKGROUND: China has experienced a continuous decreasing trend in the incidence of hepatitis A in recent years. Temporal trend analyses are helpful in exploring the reasons for the changing trend. Thus, this study aims to analyse the incidence trend of viral hepatitis A by region and age group in mainland China from 2004 to 2017 to evaluate the effectiveness of prevention and control measures. METHODS: Data on hepatitis A and population information were collected and analysed with a joinpoint regression model. Annual percentage changes (APCs) and average annual percentage changes (AAPCs) were estimated for the whole country and for each region and age group. RESULTS: From 2004 to 2017, the seasonality and periodicity of hepatitis A case numbers were obvious before 2008 but gradually diminished from 2008 to 2011 and disappeared from 2012-2017. The national incidence of hepatitis A (AAPC = - 12.1%) and the incidence rates for regions and age groups showed decreasing trends, with differences in the joinpoints and segments. Regarding regions, the hepatitis A incidence in the western region was always the highest among all regions, while a nonsignificant rebound was observed in the northeastern region from 2011 to 2017 (APC = 14.2%). Regarding age groups, the hepatitis A incidence showed the fastest decrease among children (AAPC = - 15.3%) and the slowest decrease among elderly individuals (AAPC = - 6.6%). Among all segments, the hepatitis A incidence among children had the largest APC value in 2007-2017, at - 20.4%. CONCLUSION: The national annual incidence of hepatitis A continually declined from 2004 to 2017 and the gaps in hepatitis A incidence rates across different regions and age groups were greatly narrowed. Comprehensive hepatitis A prevention and control strategies, including the use of routine vaccination during childhood in mainland China, especially the implementation of the national Expanded Program on Immunization (EPI) in 2008, resulted in substantial progress from 2004 to 2017. However, gaps remain. Regular monitoring and analysis of hepatitis A epidemic data and prompt adjustment of hepatitis A prevention and control strategies focusing on children, elderly individuals and those living in certain regions are recommended.


Assuntos
Hepatite A , Polipose Adenomatosa do Colo , Idoso , Criança , China/epidemiologia , Hepatite A/epidemiologia , Humanos , Programas de Imunização , Incidência , Análise de Regressão
18.
Chem Sci ; 13(28): 8334-8340, 2022 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-35919708

RESUMO

Accurate characterization of the longitudinal (along the thickness direction) carrier transport property is of significant importance for evaluating the quality and performance of perovskite thin films. Herein, we report the development of a modified transient reflection (TR) spectroscopy method to realize the direct observation and determination of the longitudinal carrier transport process in MAPbI3 polycrystalline thin films. Unlike the traditional TR spectroscopy, the carrier transport dynamics along the film thickness is resolved by making the pump (excitation) and probe beams spatially separated on each side of the film, so that the carrier transport from the excitation side to the probe side is directly captured. Utilizing this method, the longitudinal carrier diffusion coefficients (D) in various perovskite films with different thicknesses and grain sizes (extracted from SEM images) are determined, showing D values of ∼1.5 to 1.8 cm2 s-1 (∼0.5 to 0.8 cm2 s-1) for films with grain size larger (smaller) than the thickness. This empirical correlation between the longitudinal D and film thickness/grain size provides a reference for quick quality screening and evaluation of perovskite polycrystalline thin films.

19.
Front Genet ; 13: 926511, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35923705

RESUMO

OCA (oculocutaneous albinism) refers to a group of heterogeneous congenital disorders of which the common manifestations are variable degrees of cutaneous hypopigmentation and significant visual impairment, including poor visual acuity, photophobia, and nystagmus. Molecular analysis may elucidate its pathogenesis and be in favor of accurate diagnosis. High-throughput sequencing and Sanger sequencing were performed to detect mutational alleles and in silico analysis was performed for prediction of variant pathogenicity. Ten TYR-related and two OCA2-related patients were identified with 16 different variants with potential pathogenicity. Two novel missense variants [TYR: c.623T > G, p(Leu208Arg) and OCA2: c.1325A > G, p(Asn442Ser)] are identified in this study, and three OCA cases are reported for the first time in Chinese population based on their associated variants. Analysis of crystal structures of TYR ortholog and its paralog TYRP1 suggests that the substitution of Leu208 may have an impact on protein stability. This study may facilitate OCA diagnosis by expanding the mutational spectrum of TYR and OCA2 as well as further basic studies about these two genes.

20.
J Inflamm Res ; 15: 4275-4290, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35923903

RESUMO

Sepsis is an abnormal condition with multiple organ dysfunctions caused by the uncontrolled infection response and one of the major diseases that seriously hang over global human health. Besides, sepsis is characterized by high morbidity and mortality, especially in intensive care unit (ICU). Among the numerous subsequent organ injuries of sepsis, myocardial injury is one of the most common complications and the main cause of death in septic patients. To better manage septic inpatients, it is necessary to understand the specific mechanisms of sepsis induced myocardial injury (SIMI). Therefore, this review will elucidate the pathophysiology of SIMI from the following certain mechanisms: apoptosis, mitochondrial damage, autophagy, excessive inflammatory response, oxidative stress and pyroptosis, and outline current therapeutic strategies and potential approaches in SIMI.

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