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1.
Bioact Mater ; 21: 157-174, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36093325

RESUMO

Nowadays, infectious diseases persist as a global crisis by causing significant destruction to public health and the economic stability of countries worldwide. Especially bacterial infections remain a most severe concern due to the prevalence and emergence of multi-drug resistance (MDR) and limitations with existing therapeutic options. Antibacterial photodynamic therapy (APDT) is a potential therapeutic modality that involves the systematic administration of photosensitizers (PSs), light, and molecular oxygen (O2) for coping with bacterial infections. Although the existing porphyrin and non-porphyrin PSs were effective in APDT, the poor solubility, limited efficacy against Gram-negative bacteria, and non-specific distribution hinder their clinical applications. Accordingly, to promote the efficiency of conventional PSs, various polymer-driven modification and functionalization strategies have been adopted to engineer multifunctional hybrid phototherapeutics. This review assesses recent advancements and state-of-the-art research in polymer-PSs hybrid materials developed for APDT applications. Further, the key research findings of the following aspects are considered in-depth with constructive discussions: i) PSs-integrated/functionalized polymeric composites through various molecular interactions; ii) PSs-deposited coatings on different substrates and devices to eliminate healthcare-associated infections; and iii) PSs-embedded films, scaffolds, and hydrogels for regenerative medicine applications.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121899, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36179564

RESUMO

Recently, through space charge transfer (TSCT)-based thermally activated delayed fluorescence (TADF) molecules have shown advantages in achieving high efficiencies and tunable emissions. However, the relationships between basic molecular structures and luminescent properties are unclear. Theoretical investigations to reveal the substitution effects with different numbers and positions on excited-state properties are highly desired. Herein, by taking TSCT-based TADF molecules S-CNDF-S-tCz, S-CNDF-D-tCz and T-CNDF-T-tCz as skeletons, a series of promising TADF molecules are designed by adopting ortho, meta and para substitutions with different numbers and positions. Photophysical properties of total 16 molecules are theoretically studied by density functional theory (DFT) and time-dependent density functional theory (TD-DFT) methods in chloroform combined with polarizable continuum model. Results indicate that molecules with ortho-substitution possess small geometric changes and short Donor-Acceptor distances which are induced by the intramolecular van der Waals interactions. Decreased non-radiative consumption and increased TSCT ratio and therefore excellent performance for them can be expected. For molecules with large substitution numbers, twist structures facilitate them to realize small adiabatic energy gaps between the lowest singlet excited state (S1) and the lowest triplet excited state (T1), this designing strategy is consistent with the TADF dendrimers. Thus, the relationships between molecular structures and luminescent properties are revealed and promising TSCT-based TADF molecules with high efficiencies are theoretically proposed. Our investigations provide theoretical perspectives for inner mechanisms of substitution effect, which could further afford meaningful guidance to design new efficient TSCT-based TADF molecules.

3.
Bioact Mater ; 20: 1-15, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35633878

RESUMO

Healing of an anterior cruciate ligament (ACL) autologous graft in a bone tunnel occurs through the formation of fibrovascular scar tissue, which is structurally and compositionally inferior to normal fibrocartilaginous insertion and thus may increase the reconstruction failure and the rate of failure recurrence. In this study, an injectable hydroxyapatite/type I collagen (HAp/Col Ⅰ) paste was developed to construct a suitable local microenvironment to accelerate the healing of bone-tendon interface. Physicochemical characterization demonstrated that the HAp/Col Ⅰ paste was injectable, uniform and stable. The in vitro cell culture illustrated that the paste could promote MC3T3-E1 cells proliferation and osteogenic expression. The results of a canine ACL reconstruction study showed that the reconstructive ACL had similar texture and color as the native ACL. The average width of the tunnel, total bone volume, bone volume/tissue volume and trabecular number acquired from micro-CT analysis suggested that the healing of tendon-bone interface in experimental group was better than that in control group. The biomechanical test showed the maximal loads in experimental group achieved approximately half of native ACL's maximal load at 24 weeks. According to histological examination, Sharpey fibers could be observed as early as 12 weeks postoperatively while a typical four-layer transitional structure of insertion site was regenerated at 48 weeks in the experimental group. The injectable HAp/Col Ⅰ paste provided a biomimetic scaffold and microenvironment for early cell attachment and proliferation, further osteogenic expression and extracellular matrix deposition, and in vivo structural and functional regeneration of the tendon-bone interface.

4.
Neural Regen Res ; 18(5): 1118-1123, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36255001

RESUMO

Studies have shown that human hair keratin (HHK) has no antigenicity and excellent mechanical properties. Schwann cells, as unique glial cells in the peripheral nervous system, can be induced by interleukin-1ß to secrete nerve growth factor, which promotes neural regeneration. Therefore, HHK with Schwann cells may be a more effective approach to repair nerve defects than HHK without Schwann cells. In this study, we established an artificial nerve graft by loading an HHK skeleton with activated Schwann cells. We found that the longitudinal HHK microfilament structure provided adhesion medium, space and direction for Schwann cells, and promoted Schwann cell growth and nerve fiber regeneration. In addition, interleukin-1ß not only activates Schwann cells, but also strengthens their activity and increases the expression of nerve growth factors. Activated Schwann cells activate macrophages, and activated macrophages secrete interleukin-1ß, which maintains the activity of Schwann cells. Thus, a beneficial cycle forms and promotes nerve repair. Furthermore, our studies have found that the newly constructed artificial nerve graft promotes the improvements in nerve conduction function and motor function in rats with sciatic nerve injury, and increases the expression of nerve injury repair factors fibroblast growth factor 2 and human transforming growth factor B receptor 2. These findings suggest that this artificial nerve graft effectively repairs peripheral nerve injury.

5.
Neuropharmacology ; 222: 109305, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36354092

RESUMO

Depression is a well-known serious mental illness, and the onset of treatment using traditional antidepressants is frequently delayed by several weeks. Moreover, numerous patients with depression fail to respond to therapy. One major breakthrough in antidepressant therapy is that subanesthetic ketamine doses can rapidly alleviate depressive symptoms within hours of administering a single dose, even in treatment-resistant patients. However, specific mechanisms through which ketamine exerts its antidepressant effects remain elusive, leading to concerns regarding its rapid and long-lasting antidepressant effects. N-methyl-d-aspartate receptor (NMDAR) antagonists like ketamine are reportedly associated with serious side effects, such as dissociative symptoms, cognitive impairment, and abuse potential, limiting the large-scale clinical use of ketamine as an antidepressant. Herein, we reviewed the pharmacological properties of ketamine and the mechanisms of action underlying the rapid antidepressant efficacy, including the disinhibition hypothesis and synaptogenesis, along with common downstream effector pathways such as enhanced brain-derived neurotrophic factor and tropomyosin-related kinase B signaling, activation of the mechanistic target of rapamycin complex 1 and transforming growth factor ß1. We focused on evidence supporting the relevance of these potential mechanisms of ketamine and its metabolites in mediating the clinical efficacy of the drug. Given its reported antidepressant efficacy in preclinical studies and limited undesirable adverse effects, (R)-ketamine may be a safer, more controllable, rapid antidepressant. Overall, understanding the potential mechanisms of action of ketamine and its metabolites in combination with pharmacology may help develop a new generation of rapid antidepressants that maximize antidepressant effects while avoiding unfavorable adverse effects. This article is part of the Special Issue on 'Ketamine and its Metabolites'.


Assuntos
Disfunção Cognitiva , Ketamina , Humanos , Ketamina/farmacologia , Ketamina/uso terapêutico , Depressão , Psicoterapia , Transtornos Dissociativos
6.
Microbiol Res ; 266: 127222, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36306681

RESUMO

Acinetobacter baumannii is a ubiquitous opportunistic pathogen usually with low virulence. In recent years, reports of increased pathogenicity of A. baumannii in livestock due to the migratory behaviour of wildlife have attracted public health attention. Our previous study reported that an A. baumannii strain isolated from dead chicks, CCGGD201101, showed enhanced pathogenicity, but the mechanism for increased virulence is not understood. Here, to screen potential virulence factors, the proteomes of the isolated strain CCGGD201101 and the standard strain ATCC19606 of A. baumannii were compared, and the possible virulence-enhancing mechanisms were further analysed. The 50 % lethal dose (LD50) values of CCGGD201101 and standard strain ATCC19606 in ICR mice were determined to verify their bacterial toxicity. 2D fluorescence difference gel electrophoresis (2D-DIGE) combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) and quantitative real-time PCR (RTqPCR) were applied to screen and identify differentially expressed proteins or genes that may be related to virulence enhancement. Bioinformatics analyses based on proteinprotein interaction (PPI) networks were used to explore the function of potential virulence proteins. The pathogenicity of potential virulence factors was assessed by phylogenetic analyses and an animal infection model. The results showed that the LD50 of CCGGD201101 for mice was 1.186 × 106 CFU/mL, and the virulence was increased by 180.5-fold compared to ATCC19606. Forty-seven protein spots were significantly upregulated for the A. baumannii CCGGD201101 strain (fold change ≥1.5, p < 0.05). In total, 14 upregulated proteins were identified using proteomic analysis, and the mRNA expression levels of these proteins were nearly identical, with few exceptions. According to the PPI network and phylogenetic analyses, the I78 family peptidase inhibitor, 3-oxoacyl-ACP reductase FabG, and glycine zipper were screened as being closely related to the pathogenicity of bacteria. Furthermore, the I78 overexpression strains exhibited higher lethality in mouse infection models, which indicated that the I78 family peptidase inhibitor was a potential new virulence factor to enhance the pathogenicity of the A. baumannii CCGGD201101 strain. The present study helped us to better understand the mechanisms of virulence enhancement and provided a scientific basis for establishing an early warning system for enhanced virulence of A. baumannii from animals.


Assuntos
Acinetobacter baumannii , Camundongos , Animais , Acinetobacter baumannii/genética , Virulência/genética , Proteômica , Filogenia , Camundongos Endogâmicos ICR , Fatores de Virulência/genética , Fatores de Virulência/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Inibidores de Proteases
7.
Sci Total Environ ; 857(Pt 1): 159369, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36228793

RESUMO

BACKGROUND: Previous studies have shown that extreme heat likely increases the risk of road injuries. However, the global burden of road injuries due to high temperature and contributing factors remain unclear. This study aims to characterize the global, regional and national burden of road injuries due to high temperature from 1990 to 2019. METHODS: Based on the Global Burden of Disease (GBD) study 2019, we obtained the numbers and age-standardized mortality rates (ASMR) and age-standardized disability-adjusted life years (DALY) rates (ASDR) of the road injury due to high temperature at global, regional, and national levels from 1990 to 2019. The world is divided into five climate zones according to the average annual temperature of each country: tropical, subtropical, warm temperate, cold temperate, and boreal. We used the generalized additive models (GAM) to model the trends of road injuries globally and by region. RESULTS: Globally, between 1990 and 2019, the deaths of road injury attributable to high temperature increased significantly from 20,270 (95% uncertainty interval [UI], 7836 to 42,716) to 28,396 (95% UI, 13,311 to 51,178), and the DALYs increased from 1,169,309 (95% UI, 450,834 to 2,491,075) to 1,414,527 (95% UI, 658,347 to 2,543,613). But the ASMR and the ASDR slightly decreased by 8.49% and 13.16%, respectively. The burden of road injury death attributable to high temperature remained high in low SDI and tropical regions. In addition, road transport infrastructure investment per inhabitant is associated with the burden of road injuries attributable to high temperature. CONCLUSIONS: Globally, the ASMR and ASDR for road injuries attributable to high temperature decreased from 1990 to 2019, but the absolute death and DALYs continued to increase. Thus, concerning global warming, implementation of prevention and interventions to reduce road injuries from heat exposure should be stressed globally.


Assuntos
Saúde Global , Temperatura Alta , Humanos , Anos de Vida Ajustados por Qualidade de Vida , Temperatura , Febre , Mudança Climática
8.
Sci Total Environ ; 857(Pt 1): 159339, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36228798

RESUMO

To avoid the spread of COVID-19, China implemented strict prevention and control measures, resulting in dramatic variations in urban and regional air quality. With the complex effect from long-term emission mitigation and meteorology variation, an accurate evaluation of the net effect from lockdown on air quality changes has not been fully quantified. Here, we combined machine learning algorithm and Theil-Sen regression technique to eliminate meteorological and long-term trends effects on air pollutant concentrations and precisely detect concentrations changes those ascribed to lockdown measures in North China. Our results showed that, compared to the same period in 2015-2019, the adverse meteorology during the lockdown period (January 25th to March 15th) in early 2020 increased PM2.5 concentration in North China by 9.8 %, while the reduction of anthropogenic emissions led to a 32.2 % drop. Stagnant meteorological conditions have a more significant impact on the ground-level air quality in the Beijing-Tianjin-Hebei Region than that in Shanxi and Shandong provinces. After further striping out the effect of long-term emission reduction trend, the lockdown-derived NO2, PM2.5, and O3 shown variety change trend, and at -30.8 %, -27.6 %, and +10.0 %, respectively. Air pollutant changes during the lockdown could be overestimated up to 2-fold without accounting for the influences of meteorology and long-term trends. Further, with pollution reduction during the lockdown period, it would avoid 15,807 premature deaths in 40 cities. If with no deteriorate meteorological condition, the total avoided premature should increase by 1146.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , COVID-19 , Humanos , COVID-19/epidemiologia , Material Particulado/análise , Saúde Pública , Monitoramento Ambiental/métodos , Controle de Doenças Transmissíveis , Poluição do Ar/análise , Poluentes Atmosféricos/análise , Cidades , China/epidemiologia , Aprendizado de Máquina
9.
Chemosphere ; 310: 136753, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36216114

RESUMO

Combining photocatalysis (PC) and membrane filtration (MF) has emerged as an attractive technology for water purification, however, the water purification efficiency and membrane fouling are still challenging. Herein, we report a novel photoelectrocatalytic (PEC) membrane mediated by a ternary polyvinylidene fluoride (PVDF)-carbon black (CB)-TiO2 composite conductive membrane synthesized by a phase inversion method assisted by the mixed surfactants of polyvinylpyrrolidone (PVP) and sodium dodecyl sulfate (SDS). The resultant electrically conductive TiO2/CB/PVDF membrane features a homogeneous surface with obvious pore size of 20-150 nm, a thickness ∼116 µm, and an average resistivity as low as ∼3.165 Ω∙m. The cooperation of PVP and SDS surfactants dramatically improves the organic-inorganic interactions and thus eventually enhances the porosity, stability of porous structure, mechanical stability, and conductivity and electrochemical properties of the hybrid membrane. Upon the solvent evaperation of the wellblended casting solution and the phase inversion, TiO2/CB preferentially exist on the surface of PVDF membrane, enabling the efficient PEC degradation of organic pollutants. The synergistic coupling of TiO2 and CB in PVDF membrane results in efficient PEC properties with bi-functional membrane antifouling and enhanced water purification in azo dyes decolorization under the stationary mode and in our lab-made continuous cross-flow PEC system, superior to those by photocatalysis and electrocatalysis. The developed synchronous MF and PEC system mediated by the conductive TiO2/CB/PVDF membrane proves to a feasible route to improving the self-cleaning properties of the polymer membrane while simultaneously increasing the water decontaminating efficiency.

10.
J Hazard Mater ; 444(Pt A): 130327, 2023 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-36434919

RESUMO

The electrochemical chlorination of urea to CO2 and N2 end-products, via active-chlorine-mediated oxidation under nearly neutral conditions, is an effective treatment for medium-concentrated urea-containing wastewater. Herein, we design a novel flow reactor integrated with three-dimensional hierarchically porous Ru/RuO2 architectures anchored on a Ti mesh. The hierarchically macroporous electrode can create sufficient exposure of catalytically active sites and facilitate the microscopic mass transport and diffusion inside the active layer, thereby contributing to the increased removal efficiency of urea-N and ammonia-N. The combined results of electrochemical measurements, UV-visible spectrometry and in situ Raman spectrometry, show that the OCl- species produced by chlorine evolution reaction (CER) are the main active constituents for removing urea-N. Theoretical calculations reveal thLTWAat the Ru/RuO2 possesses a moderate Cl binding strength, lower theoretical overpotentials of CER and a higher conductivity, compared with pure RuO2. On this basis, we assemble a circular flow reactor with the hierarchically porous electrodes in a two-electrode system to obtain an enhanced microfluidic process, which during 9 days of uninterrupted operation, at a high electrolysis current of 500 mA, achieve a total nitrogen removal of 92.6% and an energy consumption of 7.94 kWh kg-1 N, demonstrating the promising application of the novel process.


Assuntos
Amônia , Cloro , Ureia , Porosidade , Halogênios , Cloretos
11.
Neural Regen Res ; 18(4): 849-855, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36204853

RESUMO

The mitochondrial permeability transition pore is a nonspecific transmembrane channel. Inhibition of mitochondrial permeability transition pore opening has been shown to alleviate mitochondrial swelling, calcium overload, and axonal degeneration. Cyclophilin D is an important component of the mitochondrial permeability transition pore. Whether cyclophilin D participates in mitochondrial impairment and axonal injury after intracerebral hemorrhage is not clear. In this study, we established mouse models of intracerebral hemorrhage in vivo by injection of autologous blood and oxyhemoglobin into the striatum in Thy1-YFP mice, in which pyramidal neurons and axons express yellow fluorescent protein. We also simulated intracerebral hemorrhage in vitro in PC12 cells using oxyhemoglobin. We found that axonal degeneration in the early stage of intracerebral hemorrhage depended on mitochondrial swelling induced by cyclophilin D activation and mitochondrial permeability transition pore opening. We further investigated the mechanism underlying the role of cyclophilin D in mouse models and PC12 cell models of intracerebral hemorrhage. We found that both cyclosporin A inhibition and short hairpin RNA interference of cyclophilin D reduced mitochondrial permeability transition pore opening and mitochondrial injury. In addition, inhibition of cyclophilin D and mitochondrial permeability transition pore opening protected corticospinal tract integrity and alleviated motor dysfunction caused by intracerebral hemorrhage. Our findings suggest that cyclophilin D is used as a key mediator of axonal degeneration after intracerebral hemorrhage; inhibition of cyclophilin D expression can protect mitochondrial structure and function and further alleviate corticospinal tract injury and motor dysfunction after intracerebral hemorrhage. Our findings provide a therapeutic target for preventing axonal degeneration of white matter injury and subsequent functional impairment in central nervous diseases.

12.
Sci Total Environ ; 856(Pt 1): 159005, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36162582

RESUMO

BACKGROUND: Injuries among preschool children are an important public health concern worldwide. Significant gaps remain in understanding the potential impact of wind speed on injuries among preschoolers. We aimed to clarify the association and its variation across subgroups to capture the vulnerability features. METHODS: Using a case-crossover design and conditional logistic regression model, we compared the exposure to wind speed right before the injury events (case period) with that of control periods to determine the excess rate (ER) of injury on each of 0-3 lag days in Guangzhou, 2016-2020. Results were also stratified by sociodemographic characteristics of patients, basic characteristics of injury events, and clinical features of injuries to identify the most vulnerable subgroups of preschoolers. RESULTS: Higher wind speed was significantly associated with an increased risk of injuries among preschoolers on lag 0, reaching an ER of 2.93 % (95 % confidence interval [CI] = 0.87, 5.03), but not on other lag days. The results of the stratified analyses showed that children under 3-year-old (3.41 %; 95 % CI = 0.36, 6.55), boys (3.66 %; 95 % CI = 1.04, 6.35), and non-locally registered children (3.65; 95 % CI = 0.02, 7.40) were more prone to wind-related injuries. Falls (2.67 %; 95 % CI = 0.11, 5.30) were the main cause of wind-related injuries, and taking transportation was the main activity when injuries occurred (13.16 %; 95 % CI = 4.45, 22.60). Additionally, injuries involving buildings/grounds/obstacles (4.69 %; 95 % CI = 1.66, 7.81) and the occurrence of sprain/strain (7.60 %; 95 % CI = 0.64, 15.04) showed a positive association with wind speed. CONCLUSIONS: Higher wind speed was associated with a significantly elevated rate of injuries among preschoolers without delayed effects, where children under 3-year-old, boys, and non-locally registered subgroups were more susceptible to wind-related injuries. This study may provide new insights for refining the prevention measures against wind-related injuries among preschoolers.


Assuntos
Vigilância de Evento Sentinela , Vento , Masculino , Humanos , Pré-Escolar , Estudos Cross-Over
13.
Sci Total Environ ; 856(Pt 1): 159010, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36174681

RESUMO

Earthworm conversion is an eco-friendly biological process that converts livestock waste into a benign nutrient-rich organic fertilizer. However, little is known about the impacts of earthworm-converted livestock manure on the antibiotic resistome in the earthworm gut microbiota. Herein, lab-scale vermicomposting was performed to comprehensively evaluate the shift of antibiotic resistance genes (ARGs) in the earthworm gut-feeding on cow manure (CM)-by metagenomic analysis. The effects of copper (Cu) as a food addictive were also evaluated. CM substantially enriched the antibiotic resistome in the foregut and midgut, while it decreased in the hindgut. A similar trend was observed for metal resistance genes (MRGs). Notably, Cu in the CM had little effect on composition of ARGs and MRGs in earthworm gut. The earthworm gut microbiome altered by CM was responsible for the shift of ARGs and MRGs. In wormcast, Cu (100 and 300 mg/kg) significantly increased the abundance of ARGs and MRGs. Our study provides valuable insight into the response of ARGs and MRGs to CM in earthworm gut, and underscores the need for the judicious use of heavy metals as feed additives in livestock and poultry farming.


Assuntos
Esterco , Oligoquetos , Animais , Feminino , Bovinos , Antibacterianos/farmacologia , Genes Bacterianos , Resistência Microbiana a Medicamentos/genética , Gado , Metais
14.
Chemosphere ; 311(Pt 1): 136914, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36272628

RESUMO

Biochar has a high specific surface area with abundant pore structure and functional groups, which has been widely used in remediation of cadmium or arsenic contaminated water and soil. However, the bottleneck problem of low-efficiency of pristine biochar in remediation of contaminated environments always occurs. Nowadays, the modification of biochar is a feasible way to enhance the performance of biochar. Based on the Web of science™, the research progress of modified biochar and its application in remediation of cadmium or arsenic contaminated water and soil have been systematically summarized in this paper. The main modification strategies of biochar were summarized, and the variation of physicochemical properties of biochar before and after modification were illustrated. The efficiency and key mechanisms of modified biochar for remediation of cadmium or arsenic contaminated water and soil were expounded in detail. Finally, some constructive suggestions were given for the future direction and challenges of modified biochar research.


Assuntos
Arsênio , Recuperação e Remediação Ambiental , Poluentes do Solo , Cádmio/análise , Arsênio/análise , Solo , Poluentes do Solo/análise , Carvão Vegetal/química , Água
15.
Front Genet ; 13: 1050192, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36452160

RESUMO

Pelteobagrus vachelli is a freshwater fish with high economic value, but the lack of genome resources has severely restricted its industrial development and population conservation. Here, we constructed the first chromosome-level genome assembly of P. vachelli with a total length of approximately 662.13 Mb and a contig N50 was 14.02 Mb, and scaffolds covering 99.79% of the assembly were anchored to 26 chromosomes. Combining the comparative genome results and transcriptome data under environmental stress (high temperature, hypoxia and Edwardsiella. ictaluri infection), the MAPK signaling pathway, PI3K-Akt signaling pathway and apelin signaling pathway play an important role in environmental adaptation of P. vachelli, and these pathways were interconnected by the ErbB family and involved in cell proliferation, differentiation and apoptosis. Population evolution analysis showed that artificial interventions have affected wild populations of P. vachelli. This study provides a useful genomic information for the genetic breeding of P. vachelli, as well as references for further studies on fish biology and evolution.

16.
Anal Chem ; 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36458654

RESUMO

The illegal additions of chemicals in food products are serious incidents threatening current public safety. To date, ideal methods to determine permanent traces of prohibited chemicals in foods are still lacking. For example, formaldehyde (FA) can be added illegally as a food preservative. However, most current methods that are dependent on the direct detection of FA are not able to determine if FA has ever been added once food products are rinsed completely. Herein, we present a novel approach relying upon protein modifications induced by FA (PMIF) to examine FA in foods. We reveal the entire catalog of PMIFs in food products by combining mass spectrometry analysis with unrestrictive identification of protein modifications. Consequently, four obvious PMIFs were identified and confirmed as markers to discriminate the addition of FA in foods. Our study demonstrates that the approach based on PMIFs enables detecting the imprinted trace of FA even if the food products have been washed thoroughly. Our work presents a novel strategy for analysis of chemical additives, offering broad potential applications in protein analysis and food safety.

17.
J AOAC Int ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36453858

RESUMO

BACKGROUND: Cyanogenic glycosides are secondary metabolites in plants. In almonds and apricot kernels, amygdalin is an abundant cyanogenic glycoside. Upon consumption, amygdalin is enzymatically metabolized into hydrogen cyanide. Depending on the number of kernels consumed and the amygdalin concentration, ingestion of amygdalin-containing kernels may result in adverse effects. To better understand the U.S. marketplace, the development and validation of analytical methods to reliably measure amygdalin in apricot kernels and almonds is needed to support the collection of occurrence and consumption data in retail products. OBJECTIVE: The aim of this study was to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitation of amygdalin in apricot kernels and almonds following the FDA Foods Program Guidelines for the Validation of Chemical Methods, 3rd Edition. METHODS: Apricot kernels and almonds were cryogenically homogenized and extracted using methanol containing an internal standard (IS), geniposide, followed by filtration, dilution, and LC-MS/MS analysis. Matrix effects were minimized using dilution. Quantitation was achieved using an external, solvent-based calibration. RESULTS: The amygdalin response was linear (r2> 0.99) over a range of 0.05-50 µg/mL. The recovery of amygdalin spiked at 10 - 10,000 µg/g in sweet apricot kernels, raw almond, and dry roasted almond ranged from 90-107% with RSDs ≤ 6%. The method limit of detection (LOD) and limit of quantitation (LOQ) was 0.8 and 2.5 ng/g, respectively. Amygdalin concentrations in 18 market samples ranged from 2- 24,000 µg/g. Corresponding estimates of cyanide concentration ranged from 0.2-1420 µg/g. CONCLUSIONS: Method performance meets the acceptance criteria defined by FDA guidelines and is fit for purpose for the analysis of amygdalin in apricot kernels and almonds. HIGHLIGHTS: An LC-MS/MS method is developed for the quantification of amygdalin in apricot kernels and almonds.

18.
J Robot Surg ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36454433

RESUMO

The original stereoelectroencephalography frame-based implantation technique has been proven to be safe and effective. But this procedure is complicated and time-consuming. With the development of modern robotic technology, robot-guided intracerebral electrodes implantation is being implemented at many epilepsy centers. We retrospectively analyzed the results of 147 patients who underwent SEEG electrode implantation surgery at our hospital. Robot-guided surgery was performed on 87 patients from January 2018 to December 2019. The remaining 60 patients received frame-based surgery from June 2015 to June 2016. 147 patients underwent a total of 149 SEEG electrode implantation procedures. The mean error of the entry point of the robot-guided surgery group was lower than that of the frame-based surgery group (1.48 ± 1.46 mm vs. 1.59 ± 0.9 mm, P < 0.001). Also, the robot group had a higher mean number of electrodes per patient (8.9 ± 2.2 vs. 7.9 ± 2.5, P = 0.004), a significantly shorter mean operative time (69.5 ± 23.3 min vs. 106.8 ± 39.8 min, P < 0.001), and mean time per electrode (7.9 ± 1.3 min vs. 13.5 ± 3.1 min, P < 0.001) than the frame-based group. In the robot-guided group, the target point (TP) error was positively correlated with skull thickness (P = 0.001) and negatively correlated with the electrode-skull angle (P = 0.041). The mean target point error and hemorrhage rates were also analyzed, but no differences were observed between the two groups. Robot-guided surgery has a higher entry point accuracy and efficiency. Electrode implantation accuracy was affected by the skull thickness and electrode-skull angle.

19.
Anim Reprod Sci ; 247: 107152, 2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36463708

RESUMO

Innate immunity is involved in ovarian activity through aseptic inflammation and tissue repair. High-mobility group box-1 (HMGB1) is related to placental inflammation and a driver of inflammation throughout pregnancy. The aim of this study was to investigate the interaction of HMGB1 with TLR2 in bovine ovarian granulosa cells, and the effects of HMGB1 on bovine ovarian granulosa cells in vitro. The viability of granulosa cells were not affected by HMGB1 with the concentration less than 5 µg/mL. The mRNA levels of TLR2, epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF), the steroidogenic acute regulatory protein (StAR), tissue inhibitors of matrix (TIMP1/2) of ovarian granulosa cells were upregulated by HMGB1(P < 0.05). The protein levels of TLR2, TLR1 and phosphorylation-NF-κB (p-NF-κB) p65 in ovarian granulosa cells increased in 5 µg/mL HMGB1 group (P < 0.05), and TLR2 decreased in siRNA-2 group (P < 0.05). IL-6 of ovarian granulosa cells was increased by 1 µg/mL and 5 µg/mL HMGB1 (P < 0.05). These results implicate that HMGB1 has interaction with TLR2, TLR1 and p-NF-κB p65 in ovarian granulosa cells, which lead to nuclear translocation of NF-κB p65 and the secretion of interleukin-6 (IL-6). HMGB1 regulates the expression of EGFR, VEGF, StAR, TIMP1/2 and the secretion of IL-6 in ovarian granulosa cells of dairy cows through activating the TLR2/NF-κB signaling pathway, which may be interfere with ovarian physiological activity.

20.
Environ Pollut ; : 120735, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36464113

RESUMO

Iron-manganese-based adsorbent has been regarded as a promising candidate for arsenic purification from water, especially the inorganic As(III), due to its inherent advantage of low cost and large-scale producibility. However, the nanoparticle aggregation, metal leaching and insufficient removal efficiency remain the main challenges in the practical applications of the granular adsorbents. In this work, we develop a universal strategy for the fabrication of an active Fe(III) oxyhydroxide-Mn(IV) oxide/3D graphene oxide (GO) gel composite via a simple hydrothermal reaction. The successful immobilization of Fe-Mn oxyhydroxide/oxides on the interconnected GO gels was intuitively confirmed by the transmission electron microscopy and atomic force microscopy. The combinative characterizations of the X-ray absorption near edge structure and X-ray photoelectron spectroscopy clearly reveal the electron transfer from Fe atoms to Mn atoms. The optimized Fe-Mn/GO composites possess the superior performance with the removal efficiency of over 90% for As(III) at pH 7.0 and ∼97% for As(V) at pH 5.0 and the As(III, V) levels (100 µg l-1) are reduced to below the WHO guideline of 10 µg l-1. The sorption isotherm and kinetic experiments on the As removal were also carried out. The post characterizations are employed to better unveil the oxidation-adsorption mechanism. Notably, the application of Fe-Mn/GO composites in the treatment of As-simulated natural water demonstrated a stable and continuous operation for over 20 days and an effluent concentration of arsenic as low as the 10 µg l-1 in a specially designed flow reactor.

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