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1.
FEMS Microbiol Lett ; 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31909810

RESUMO

Gut microbiota had been suggested as an important component of host defense. However, microbiota in other parts of the body have rarely been characterized. In our present work, we give a detailed view of the body of S. exigua larvae, the midgut with intestinal contents (MG), midgut excluding intestinal contents (PM), intestinal contents (IC), and remaining parts excluding the MG (RM), with the objective of revealing the complexity of microorganisms and comparing the biodiversity of the S. exigua larvae-associated microbiota. Our results provide the first evidence that 3rd and 5th larvae samples of S.exigua were dominated by members of the phyla Firmicutes and Proteobacteria. In addition, there were no obvious differences in relative abundances of microbiota among MG, PM and RM at the phylum level. MG3 and MG5 (nearly RM5) harbored the richest microbial community, with much higher abundance of Halomonas, Pseudomonas and Methylobacterium, respectively. Intestinal contents contained the lowest diversity and highest abundance of Enterococcus. Knowledge of the bacteria in a major herbivorous insect, such as S. exigua, can facilitate the acquisition of special biological resources for agricultural pest control.

2.
Water Sci Technol ; 80(5): 920-928, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31746799

RESUMO

A novel Fenton-SBR-microwave pyrolysis integrated process is developed to treat liquid crystal wastewater possessing complex components, high toxicity and strong stability. In this integrated process, Fenton-SBR and microwave pyrolysis are for the removal of chemical oxygen demand (COD) and disposal of iron mud generated in the Fenton process respectively. The effects of H2O2:Fe2+ molar ratio and Fenton dosage on COD removal were optimized. The experimental results revealed that the removal efficiencies for COD and total organic carbon (TOC) were 99.8% and 99.2%, and the values for MLSS and SVI were stable at 4,500 mg L-1 and 65%, respectively. Microscopic examination proved that there were rotifer, Epistylis galea, Opercularia coarctata, vorticella and mormon genus which are indicative microbes for good water quality. Iron mud waste produced in the Fenton reaction was handled with microwave pyrolysis, producing ɑ-Fe2O3 commercial byproduct. The estimated cost including chemical reagents and electricity for this integrated process is about $320 T-1, without consideration of the added value of the ɑ-Fe2O3 byproduct. TOC removals in the Fenton and SBR processes both fit well with pseudo-first-order kinetics and the corresponding half-life times are 0.15 and 7 h, respectively.


Assuntos
Cristais Líquidos , Poluentes Químicos da Água , Purificação da Água , Peróxido de Hidrogênio , Micro-Ondas , Oxirredução , Pirólise , Eliminação de Resíduos Líquidos , Águas Residuárias
3.
PLoS One ; 14(11): e0224897, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31747415

RESUMO

Plant height (PH) is an important trait in soybean, as taller plants may have higher yields but may also be at risk for lodging. Many genes act jointly to influence PH throughout development. To map the quantitative trait loci (QTL) controlling PH, we used the unconditional variable method (UVM) and conditional variable method (CVM) to analyze PH data for a four-way recombinant inbred line (FW-RIL) population derived from the cross of (Kenfeng14 × Kenfeng15) × (Heinong48 × Kenfeng19). We identified 7, 8, 16, 19, 15, 27, 17, 27, 22, and 24 QTL associated with PH at 10 developmental stages, respectively. These QTL mapped to 95 genomic regions. Among these QTL, 9 were detected using UVM and CVM, and 89 and 66 were only detected by UVM or CVM, respectively. In total, 36 QTL controlling PH were detected at multiple developmental stages and these made unequal contributions to genetic variation throughout development. Among 19 novel regions discovered in our study, 7 could explain over 10% of the phenotypic variation and contained only one single QTL. The unconditional and conditional QTL detected here could be used in molecular design breeding across the whole developmental procedure.

4.
Front Plant Sci ; 10: 1230, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31636648

RESUMO

Myrosinase enzymes and their substrate glucosinolates provide a specific defensive mechanism against biotic invaders in the Brassicaceae family. In these plants, myrosinase hydrolyzes glucosinolates into diverse products, which can have direct antibiotic activity or function as signaling molecules that initiate a variety of defense reactions. A myrosinase, ß-thioglucoside glucohydrolase 1 (TGG1) was previously found to be strikingly abundant in guard cells, and it is required for the abscisic acid (ABA) response of stomata. However, it remains unknown which particular physiological processes actually involve stomatal activity as modulated by TGG1. In this experimental study, a homologous TGG1 gene from broccoli (Brassica oleracea var. italica), BoTGG1, was overexpressed in Arabidopsis. The transgenic plants showed enhanced resistance against the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 via improved stomatal defense. Upon Pst DC3000 infection, overexpressing BoTGG1 accelerated stomatal closure and inhibited the reopening of stomata. Compared with the wild type, 35S::BoTGG1 was more sensitive to ABA- and salicylic acid (SA)-induced stomatal closure but was less sensitive to indole-3-acetic acid (IAA)-inhibited stomatal closure, thus indicating these hormone signaling pathways were possibly involved in stomatal defense regulated by TGG1. Furthermore, overexpression of BoTGG1 delayed flowering by promoting the expression of FLOWERING LOCUS C (FLC), which encodes a MADS-box transcription factor known as floral repressor. Taken together, our study's results suggest glucosinolate metabolism mediated by TGG1 plays a role in plant stomatal defense against P. syringae and also modulates flowering time by affecting the FLC pathway.

5.
iScience ; 20: 137-147, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31569048

RESUMO

We developed a universal method termed OnCELISA to detect cytokine secretion from individual cells by applying a capture technology on the cell membrane. OnCELISA uses fluorescent magnetic nanoparticles as assay reporters that enable detection on a single-cell level in microscopy and flow cytometry and fluorimetry in cell ensembles. This system is flexible and can be modified to detect different cytokines from a broad range of cytokine-secreting cells. Using OnCELISA we have been able to select and sort highly cytokine-secreting cells and identify cytokine-secreting expression profiles of different cell populations in vitro and ex vivo. We show that this system can be used for ultrasensitive monitoring of cytokines in the complex biological environment of atherosclerosis that contains multiple cell types. The ability to identify and select cell populations based on their cytokine expression characteristics is valuable in a host of applications that require the monitoring of disease progression.

6.
Ann Vasc Surg ; 61: 394-399, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31352086

RESUMO

BACKGROUND: The aim of this study was to develop a diagnostic prediction model to improve identification of acute symptomatic portal vein thrombosis (PVT). METHODS: We examined 47 patients with PVT and 94 controls without PVT in the Second Affiliated Hospital of Soochow University and Suqian People's Hospital of Nanjing, Gulou Hospital Group. We constructed a prediction model by using a support vector machine (SVM) classifier coupled with a least absolute shrinkage and selection operator (LASSO). We applied a 10-fold cross-validation to estimate the error rate for each model. RESULTS: The present study indicated that acute symptomatic PVT was associated with 11 indicators, including liver cirrhosis, D-Dimer, splenomegaly, splenectomy, inherited thrombophilia, ascetic fluid, history of abdominal surgery, bloating, C-reactive protein (CRP), albumin, and abdominal tenderness. The LASSO-SVM model achieved a sensitivity of 91.5% and a specificity of 100.0%. CONCLUSIONS: We developed a LASSO-SVM model to diagnose PVT. We demonstrated that the model achieved a sensitivity of 91.5% and a specificity of 100.0%.

7.
J Cell Biochem ; 120(9): 15527-15537, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31062400

RESUMO

HOXB13 exerts a close relation in several human cancers. This study explored the role of HOXB13 in glioblastoma (GBM), a brain tissue with the highest aggressive rate and mortality in adults. Through microarray and immunohistochemistry analyses, HOXB13 was highly expressed in GBM tissues. Furthermore, we showed that high-level expression of HOXB13 in GBM was associated with worse survival, suggesting that HOXB13 could be a prognostic marker for patients with GBM. GBM cells U87 and U251 overexpressing HOXB13 showed enhanced proliferation, migration, and invasion relative to the control cells, while knockdown of HOXB13 led to decreased cell proliferation, migration, and invasion abilities. In addition, dual-luciferase report assay, chromatin immunoprecipitation assay, and quantitative real-time polymerase chain reaction data showed that HOXB13 directly bound to HOXC-AS3 promoter. HOXC-AS3 was involved in HOXB13-induced proliferation, migration, and invasion of GBM cells. In summary, this study revealed the prognostic potential of HOXB13 in GBM. We believed that HOXB13/HOXC-AS3 signaling axis can be served as therapeutic targets for this highly aggressive cancer.

8.
Am J Cancer Res ; 9(2): 250-269, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30906627

RESUMO

The aggressiveness and recurrence of glioma are major obstacles for the treatment of this type of tumor. Further understanding of the molecular mechanisms of glioma is necessary to improve the efficacy of therapy. MicroRNAs have been widely studied in many human cancers. Here, we found that miR-940 was one of the primary downregulated miRNAs in clinical samples and glioma cell lines through bioinformatics analysis and qRT-PCR. Upregulating miR-940 expression significantly inhibited the proliferation and invasion and promoted apoptosis of U87 and U118 cells. In addition, experiments in vivo showed that upregulation of miR-940 expression inhibited xenograft growth. Methylenetetrahydrofolate dehydrogenase (MTHFD2), a dual-functional metabolic enzyme, is involved in the one-carbon metabolism of folate in mitochondria. We found MTHFD2 to be overexpressed in glioma tissues and our clinical samples by qRT-PCR and Western blot assays. Through TargetScan prediction and luciferase assays, we found that miR-940 directly targets MTHFD2. Upregulation of miR-940 expression inhibited the expression of MTHFD2 and led to intracellular one-carbon metabolism dysfunction. Furthermore, the antitumor effects of miR-940 could be attenuated by overexpression of MTHFD2. Together, the results of our study suggest that miR-940 may be a new therapeutic target for the treatment of glioma through targeting of MTHFD2.

9.
Autism Res ; 12(3): 375-383, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30629339

RESUMO

Autism spectrum disorder (ASD) is a serious lifelong neurodevelopmental disorder. ASD is diagnosed for children at the age of two. ASD diagnosis, as early as possible, lays the foundation for treatment and much better prognosis. Notably, gene-based test is an inherent method to recognize the potential infants with ASD before the age of two. To investigate whether SHANK family contributes to ASD prediction, on the basis of our previous studies of SHANK2 and SHANK3, we further investigated associations between SHANK1 polymorphisms and ASD risk as well as SNP-SNP interactions among SHANK family. We enrolled 470 subjects (229 cases and 241 healthy controls) who were northeast Chinese Han. Four tag SNPs (rs73042561, rs3745521, rs4801846, and rs12461427) of SHANK1 were selected and genotyped. We used the SNPStats online analysis program to assess the associations between the four SNPs and ASD risk. The SNP-SNP interactions among SHANK family were analyzed using multifactor dimensionality reduction method. We found that the four SHANK1 SNPs were not associated with ASD risk in northeast Chinese Han population. There existed a strong synergistic interaction between rs11236697 [SHANK2] and rs74336682 [SHANK2], and moderate synergistic interactions (rs74336682 [SHANK2]-rs73042561 [SHANK1], rs11236697 [SHANK2]-rs77716438 [SHANK2], and rs11236697 [SHANK2]-rs75357229 [SHANK2]). These SHANK1 variants may not affect the susceptibility to ASD in Chinese Han population. SNP-SNP interactions in SHANK family may confer ASD risk. Autism Res 2019, 12: 375-383 © 2019 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: ASD is a serious lifelong neurodevelopmental disorder with strong genetic components. We investigated associations between SHANK1 polymorphisms and ASD risk as well as SNP-SNP interactions among SHANK family. Our results indicated that there exists no association between SHANK1 SNPs and ASD, and SNP-SNP interactions in SHANK family may confer ASD risk in the Northeast Han Chinese population. Future studies are needed to test more SHANK family SNPs in a large sample to demonstrate the associations.

10.
Pest Manag Sci ; 75(4): 1045-1055, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30242939

RESUMO

BACKGROUND: Diamondback moth, Plutella xylostella L., is a very important pest of cruciferous vegetables causing excessive economic losses worldwide. Bioactivities of halo-, diazo-, and cyclopropane acetates of P. xylostella sex pheromone have been evaluated using electrophysiology and enzyme inhibition assays. RESULTS: A total of 23 sex pheromone analogs of P. xylostella were designed and synthesized and the result shows that (11Z)-hexadec-11-en-1-yl 2,2,2-trifluoroacetate, (11Z)-hexadec-11-en-1-yl 2,2,3,3,3-pentafluoropropanoate, and (11Z)-hexadec-11-en-1-yl trifluoromethanesulfonate elicited potential inhibitory effects at all doses tested in the electrophysiology and enzyme inhibition assays. Interference of locating the sex pheromone source was found strongest when these three analogs were mixed with the sex pheromone at a 10:1 ratio. In addition, field test showed that the rate of mating disruption was over 90% when (11Z)-hexadec-11-en-1-yl 2,2,2-trifluoroacetate or (11Z)-hexadec-11-en-1-yl 2,2,3,3,3-pentafluoropropanoate was mixed with the sex pheromone at a 10:1 ratio. CONCLUSION: Two sex pheromone antagonists were screen out by electrophysiology, enzyme inhibition assays, wind tunnel and field tests. We believe that these antagonists could be used to establish a novel eco-friendly measure to control P. xylostella and provide evidence for clarifying the specific functions and molecular mechanisms of sex pheromone antagonists. © 2018 Society of Chemical Industry.


Assuntos
Mariposas/fisiologia , Feromônios/farmacologia , Atrativos Sexuais/farmacologia , Animais , Desenho de Drogas , Masculino , Mariposas/química , Feromônios/síntese química , Relação Quantitativa Estrutura-Atividade , Atrativos Sexuais/síntese química
11.
Front Plant Sci ; 9: 1690, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30519252

RESUMO

Protein content (PC), an important trait in soybean (Glycine max) breeding, is controlled by multiple genes with relatively small effects. To identify the quantitative trait nucleotides (QTNs) controlling PC, we conducted a multi-locus genome-wide association study (GWAS) for PC in 144 four-way recombinant inbred lines (FW-RILs). All the FW-RILs were phenotyped for PC in 20 environments, including four locations over 4 years with different experimental treatments. Meanwhile, all the FW-RILs were genotyped using SoySNP660k BeadChip, producing genotype data for 109,676 non-redundant single-nucleotide polymorphisms. A total of 129 significant QTNs were identified by five multi-locus GWAS methods. Based on the 22 common QTNs detected by multiple GWAS methods or in multiple environments, pathway analysis identified 8 potential candidate genes that are likely to be involved in protein synthesis and metabolism in soybean seeds. Using superior allele information for 22 common QTNs in 22 elite and 7 inferior lines, we found higher superior allele percentages in the elite lines and lower percentages in the inferior lines. These findings will contribute to the discovery of the polygenic networks controlling PC in soybean, increase our understanding of the genetic foundation and regulation of PC, and be useful for molecular breeding of high-protein soybean varieties.

12.
Gene ; 675: 197-207, 2018 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-30180966

RESUMO

Hypertension is one of the most common chronic diseases, constituting an independent risk factor for many diseases. Our study aimed to evaluate the association between apolipoprotein E (ApoE) genetic polymorphism and hypertension, and to provide evidence for the etiology of hypertension. Case-control studies of ApoE polymorphism and hypertension, which were included in PubMed, Embase, Web of Science, Medline, WanFang, Vip, and CNKI information databases, were selected and evaluated according to criteria of inclusion and exclusion. Eligible data were extracted and pooled, and were analyzed and assessed using Stata 12.0. Random-effect models were used when heterogeneity existed in between-study, and fixed-effect models were applied otherwise. A total of 28 studies that consisted of 5898 cases with hypertension and 7518 controls were selected. Alleles and genotypes of ApoE between cases and controls were compared. For ApoE alleles, we observed the contrast of ApoE ε2 versus ε3 allele yielded a pooled OR of 0.99 (95% CI: 0.87-1.11; P = 0.823), whereas the contrast of ε4 versus ε3 allele yielded a pooled OR of 1.95 (95% CI: 1.50-2.54; P < 0.001). For ApoE genotypes, compared with ε3/ε3 genotype, genotypes (ε2/ε2 and ε2/ε3) showed a possible association with hypertension (OR = 0.88; 95% CI: 0.79-0.99; P = 0.033), and genotypes (ε3/ε4 and ε4/ε4) had a 2.08-fold risk of developing hypertension (OR = 2.08; 95% CI: 1.58-2.74; P < 0.001). There is the association between ApoE polymorphism and hypertension: the genotypes carrying ε2 allele may be a protective factor, and the ApoE ε4 allele and the genotypes carrying ε4 allele may be risk factors for hypertension.


Assuntos
Apolipoproteínas E/genética , Hipertensão/genética , Polimorfismo Genético , Estudos de Casos e Controles , Predisposição Genética para Doença , Genótipo , Humanos , Análise de Regressão
13.
PLoS One ; 13(9): e0198570, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30216338

RESUMO

Saline-alkali soil is an arable land resource on which transgenic Bacillus thuringiensis (Bt) cotton has been planted on a large scale in accordance with food security strategies. There are, however, concerns about the insecticidal effects of Bt cotton on target insect pests. In this study, a Bt cotton variety, GK19, and its nontransgenic parent variety, Simian-3, were used as experimental models for investigating the effect of the expression of exogenous insecticidal proteins in Bt cotton under NaCl stress on the feeding behavior and nutritional parameters of Helicoverpa armigera. The results showed that the expression of exogenous insecticidal proteins in GK19 was significantly inhibited under NaCl stress. However, the feeding, crawling, resting and spinning down behavior of the 5th instar H. armigera larvae on GK19 Bt cotton, as well as the amount of food consumed and feces produced by these larvae, did not markedly differ under different NaCl concentrations. In contrast, the mean relative growth rate (MRGR), relative growth rate (RGR), approximate digestibility (AD), efficiency of conversion of ingested food (ECI) and efficiency of conversion of digested food (ECD) of the larvae markedly decreased in response to NaCl stress. Under the same concentration of NaCl, the nutritional parameters of the bollworm larvae on GK19 Bt cotton or Simian-3 nontransgenic cotton were different. However, the interaction between salt stress and cotton variety had no significant effect on the feeding behavior or nutritional parameters of H. armigera larvae. These results may provide a scientific basis for determining the effect of exogenous insecticidal protein expression in Bt cotton under NaCl stress on H. armigera and can therefore be useful for the effective application of Bt cotton in saline-alkali soils to prevent and control H. armigera.


Assuntos
Bacillus thuringiensis/genética , Gossypium/genética , Herbivoria , Mariposas/fisiologia , Plantas Geneticamente Modificadas/genética , Estresse Salino , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Gossypium/parasitologia , Gossypium/fisiologia , Inseticidas/metabolismo , Controle Biológico de Vetores , Plantas Geneticamente Modificadas/parasitologia , Plantas Geneticamente Modificadas/fisiologia , Cloreto de Sódio/metabolismo
14.
Clin Chim Acta ; 486: 209-213, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30096316

RESUMO

BACKGROUND: Various smoking status and high prevalence of dyslipidemia in residents exist in northeast China. However, associations of dyslipidemia with smoking status remain unclear. METHODS: A total of 17,114 participants selected by a multistage stratified cluster random sampling design were enrolled from a cross-sectional study conducted in northeast China. Associations of dyslipidemia with smoking/quitting status (smoking amount, smoking duration, and quitting duration) were investigated using multiple logistic regression. RESULTS: Prevalence (39.2%) of dyslipidemia existed in residents in northeast China. Smoking amount was associated with dyslipidemia (1-10 cigarettes daily: OR = 1.19, 95% CI: 1.08-1.32; 11-20 cigarettes daily: OR = 1.29, 95% CI: 1.16-1.42; and over 20 cigarettes daily: OR = 1.51, 95% CI: 1.25-1.83). Smoking duration was associated with dyslipidemia risk (6-10 years: OR = 1.75, 95% CI: 1.51-2.03; 11-15 years: OR = 1.85, 95% CI: 1.51-2.26; and ≥15 years: OR = 1.12, 95% CI: 1.02-1.23). Quitting duration (1-5 years) was associated with dyslipidemia (OR = 1.26, 95% CI: 1.07-1.48); however, we found no statistically significant associations between dyslipidemia and quitting duration (over 6 years). CONCLUSIONS: Dyslipidemia risk is positively associated with smoking/quitting status. Smoking amount and smoking duration may co-determine dyslipidemia risk, and quitting duration (>6 years) is necessary for reducing dyslipidemia risk.


Assuntos
Dislipidemias/epidemiologia , Abandono do Hábito de Fumar/estatística & dados numéricos , Fumar/epidemiologia , Adolescente , Adulto , Idoso , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
15.
Mol Immunol ; 101: 197-202, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30007229

RESUMO

Monoclonal antibodies (MAbs) are a unique and attractive class of biologics and are potential substitutes for post-exposure rabies prophylaxis. The safety, tolerance, and broad neutralization efficiency of a MAb cocktail called CL184, composed of the antibodies CR4098 and CR57, was confirmed in a phase I clinical trial. We have prepared a series of single-chain Fv fragments (scFvs) and leucine zipper Fv fragments (zipFvs) from CR57 and CR4098. In this study, we selected and formed scFv and zipFv cocktails and compared their protective effects against the rabies virus. Mice and hamster challenge models demonstrated the improved protection of the zipFv cocktail compared with scFv cocktail, because of its stronger affinity. The results indicate that zipFv production is a promising novel method for the genetic engineering of antibody fragments and improving affinity through systematic screening may be important when designing small molecule antibodies against RV.


Assuntos
Anticorpos Monoclonais/imunologia , Zíper de Leucina , Vírus da Raiva/imunologia , Anticorpos de Cadeia Única/imunologia , Animais , Afinidade de Anticorpos , Cricetinae , Modelos Animais de Doenças , Humanos , Camundongos , Testes de Neutralização
16.
Endocr Pract ; 24(7): 677-683, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30048165

RESUMO

OBJECTIVE: To investigate the prevalence and risk factors of impaired fasting glucose (IFG) among adults in northeast China. METHODS: A cross-sectional study was conducted in Jilin Province in 2012. Questionnaires were used to collect information about demographic characteristics, lifestyle factors, and health status from 15,540 residents. Fasting blood glucose (FBG) was measured in the morning after at least 12 hours of fasting, and χ2 tests were performed to compare differences between subjects with and without IFG. Logistic regression was carried out to identify factors influencing IFG occurrence. RESULTS: There were significant differences in demographic characteristics (age, sex, education, and marriage status), lifestyle factors (smoking, drinking, physical activity, and average sleep duration), and health status (hyperlipidemia, hypertension, and BMI category) between subjects with IFG and without IFG ( P<.05). IFG risk was significantly associated with sex, age, education (senior high school and college), marriage status (single), drinking, hyperlipidemia, hypertension, and BMI category (all P<.05). CONCLUSION: In adults in northeast China, risk factors of IFG are sex, age, education (senior high school and college), drinking, hyperlipidemia, hypertension, and BMI category; however, the protective factor of IFG is marriage status (single). ABBREVIATIONS: BMI = body mass index; CI = confidence interval; FBG = fasting blood glucose; IFG = impaired fasting glucose; OR = odds ratio; T2DM = type 2 diabetes.


Assuntos
Diabetes Mellitus Tipo 2 , Jejum , Adulto , Glicemia , China , Estudos Transversais , Humanos , Prevalência , Fatores de Risco
17.
Protein Expr Purif ; 151: 56-61, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-29908315

RESUMO

Streptococcus pneumoniae is a major pathogen that causes life-threatening diseases, such as pneumonia, otitis media, bacteremia, and meningitis, worldwide and especially in young children and the elderly. Pneumococcal surface protein A (PspA) is a widely studied candidate protein vaccine that represents a promising replacement for current polysaccharide and polysaccharide-conjugate vaccines. In this study, we describe a simple method to produce PspA of clade 4 from an Escherichia coli expression system using hydroxylapatite and ion-exchange chromatography. Using this method, we successfully expressed soluble PspA4 in 10 L of autoinducing culture medium, with a wet-cell yield of 19 g/L and a final PspA4 concentration of 22.8 mg/L. Additionally, we improved PspA4 purity from 17% to 70% in a single step through the use of hydroxylapatite, resulting in acquisition of recombinant PspA4 (>95% purity) at a final yield of 43% from the starting cell-lysis solution. We subsequently verified the secondary structure molecular weight of recombinant PspA4 by circular dichroism and mass spectrometry, respectively. These results demonstrated a highly efficient method for mass producing PspA4 protein and that can also be applied for purification of PspA proteins from other clades.


Assuntos
Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/isolamento & purificação , Durapatita/química , Escherichia coli/metabolismo , Cromatografia por Troca Iônica , Escherichia coli/genética , Fermentação , Expressão Gênica , Estrutura Secundária de Proteína , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação
18.
Brain Behav Immun ; 71: 18-22, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29678795

RESUMO

Mounting evidence indicates that cytokines secreted by innate immune cells in the brain play a central role in regulating neural circuits that subserve mood, cognition, and sickness responses. A major impediment to the study of neuroimmune signaling in healthy and disease states is the absence of tools for in vivo detection of cytokine release in the brain. Here we describe the design and application of a cytokine detection device capable of serial monitoring of local cytokine release in discrete brain regions. The immunocapture device consisted of a modified optical fiber labeled with a capture antibody specific for the pro-inflammatory cytokine interleukin-1 beta (IL-1ß). Using a sandwich immunoassay method, in vitro data demonstrate that the sensing interface of the modified optical fiber has a linear detection range of 3.9 pg mL-1-500 pg mL-1 and spatial resolution on the order of 200-450 µm. Finally, we show that the immunocapture device can be introduced into a perforated guide cannula for repeated analyte measurements in vivo. An increase in fluorescence detection of spatially localized intrahippocampal IL-1ß release was observed following a peripheral lipopolysaccharide challenge in Sprague-Dawley rats. This novel immunosensing technology represents an opportunity for unlocking the function of neuroimmune signaling.


Assuntos
Mapeamento Encefálico/instrumentação , Mapeamento Encefálico/métodos , Citocinas/metabolismo , Interleucina-1beta/metabolismo , Animais , Anticorpos , Encéfalo/imunologia , Encéfalo/metabolismo , Citocinas/análise , Imunoensaio/métodos , Interleucina-1beta/análise , Lipopolissacarídeos/farmacologia , Masculino , Fibras Ópticas , Ratos , Ratos Sprague-Dawley
19.
Gene ; 651: 100-105, 2018 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-29408620

RESUMO

Autism spectrum disorder (ASD), as one of neurodevelopmental disorders, affects about 1/160 of people worldwide. The etiology and pathogenesis of ASD remain elusive. Synapses are essential components of neurons and basic information transmission unit in the nervous system, adjusting behavior to environmental stimuli and controlling body functions, memories, and emotions. SHANK3 is one of the synapse genes which play important roles in maintaining synaptic structure and function. SHANK3 has been researched as a probably susceptibility gene for ASD. We investigated the association between polymorphisms in SHANK3 and ASD in the Northeast Han Chinese population. A total of 470 subjects (229 cases and 241 controls) were enrolled in our case-control study. Five single nucleotide polymorphisms (SNPs) (rs756638, rs4824116, rs76268556, rs9616915, and rs75767639) in SHANK3 were selected and genotyped. Our study did not identify a significant association of SHANK3 SNPs with ASD in the Northeast Han Chinese population. Future studies need to test more SHANK3 SNPs in large sample to demonstrate the association between SNPs in SHANK3 and ASD.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Transtorno do Espectro Autista/genética , Proteínas do Tecido Nervoso/genética , Polimorfismo de Nucleotídeo Único , Estudos de Casos e Controles , Pré-Escolar , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Masculino
20.
Lipids Health Dis ; 17(1): 16, 2018 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-29357896

RESUMO

BACKGROUND: Dyslipidemia and other cardiovascular disease (CVD) risk factors have a strong association with obesity. Anthropometric indices have been widely used to evaluate obesity in clinical and epidemiological studies. We aim to investigate association between serum lipid levels and different anthropometric indices. METHODS: Our study included 17,554 participants. We mainly investigated area under the receiver operating characteristic (AUROC) curves and optimal operating points (OOPs) between the anthropometric indices and serum lipid levels or categories of abnormal serum lipid indices. RESULTS: For predicting one/two categories of abnormal serum lipid indices among the anthropometric indices, AUROC value of WC was the highest in men (0.718), and AUROC values of BRI and WHtR were the highest in women (0.700 and 0.700) (all P < 0.001); OOP of WC was 82.450 in men; OOPs of BRI and WHtR were 3.435 and 0.504 in women. For predicting three/more categories of abnormal serum lipid indices among the anthropometric indices, AUROC value of WC was the highest in men (0.806), and AUROC values of BRI and WHtR were the highest in women (0.783 and 0.783) (all P < 0.001); OOP of WC was 84.150 in men; OOPs of BRI and WHtR were 3.926 and 0.529 in women. CONCLUSIONS: WC was a good predictor for one/two or three/more categories of abnormal serum lipid indices in men. However, BRI and WHtR were good predictors for one/two or three/more categories of abnormal serum lipid indices in women. ABSI showed the weakest predictive power.


Assuntos
Dislipidemias/epidemiologia , Obesidade/complicações , Circunferência da Cintura , Relação Cintura-Quadril , Adulto , Antropometria , Área Sob a Curva , China , Estudos Transversais , Dislipidemias/diagnóstico , Dislipidemias/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC
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