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1.
Artigo em Inglês | MEDLINE | ID: mdl-31922355

RESUMO

OBJECTIVE: This study aimed to evaluate the usefulness of the admission risk index (RI) to predict short-term and long-term outcomes in a broad population with ST-elevation myocardial infarction (STEMI) using data from the Chinese Acute Myocardial Infarction Registry. BACKGROUND: The RI was developed as a simple tool to predict risk of death in STEMI patients. The performance in predicting short-term and long-term risk of death in Chinese patients receiving percutaneous coronary intervention and conservative treatment for STEMI remains unclear. METHODS: Age, heart rate (HR), and systolic blood pressure (SBP) were used to calculate RI using (HR×[age/10]2 )/SBP. We used the prediction tool to predict mortality over 12 months. RESULTS: The C-index of the admission RI for predicting in-hospital, 1-, 6-, and 12-months mortality were 0.78, 0.78, 0.78, and 0.77, respectively, compared with 0.75 of the Global Registry in Acute Coronary Events score. Based on the receiver operating characteristic curve analysis, the RI was categorized into quintiles for convenient clinical use, and it revealed a nearly 15-fold gradient of increasing mortality from 2.29 to 32.5% (p < .0001) while RI >34 had the highest mortality. By categorizing into five different risk groups, the short-term and long-term mortality of patients receiving different treatments could be distinguished. CONCLUSIONS: RI based on three routine variables and easily calculated by any medical practitioner is useful for predicting in-hospital and long-term mortality in patients with STEMI at the initial consultation with clinicians.

2.
Neurochem Int ; 133: 104643, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31837353

RESUMO

Adult hippocampal neurogenesis plays the pivotal roles in central nervous system diseases. Recently, it has been reported that levetiracetam (LEV), a new antiepileptic drug with novel chemical construction and unique pharmacological properties, suppressed aberrant adult subventricular zone (SGZ) neurogenesis in kainite-induced epileptic mice, while promoted adult SGZ neuroblast differentiation in normal mice. These studies indicate LEV can modulate adult hippocampal neurogenesis, but the exact mechanism remained unknown. Thus, the present study aimed to investigate the effects of subchronic and chronic LEV treatments on neural stem cell by lineage tracing in adult hippocampal dentate gyrus of mice, as well as the potential mechanism related to Wnt/ß-catenin signaling pathway. The data showed that both subchronic and chronic LEV treatments had no effects on body weight, locomotor activity and anxiety-like behavior in mice. Notably, subchronic LEV treatment significantly suppressed the proliferation of intermediate progenitor cell and neuroblast, decreased the number of intermediate progenitor cell and neuroblast, but increased the number of quiescent neural stem cell. On the contrary, chronic LEV treatment promoted the proliferation of neural stem cell, intermediate progenitor cell and neuroblast, increased the number of neural stem cell, intermediate progenitor cell and neuroblast, and promoted differentiation of newborn immature neuron and mature neuron. Furthermore, subchronic LEV treatment decreased the level of Wnt 3a and nuclear ß-Catenin expression, which led to the inhibition on Wnt/ß-catenin signaling pathway. Chronic LEV treatment increased the level of Wnt 3a, cytosolic ß-catenin and nuclear ß-Catenin, decreased the expression of GSK-3ß, p-Tyr216-GSK-3ß and Axin2, resulting in the enhancement of Wnt/ß-catenin signaling pathway. These results demonstrated that LEV significantly suppressed or promoted adult hippocampal neurogenesis in mice by subchronic or chronic treatment possibly through the regulation of Wnt/ß-catenin signaling pathway. Our findings provided the new perspectives of LEV on adult hippocampal neurogenesis underlying its clinical application.

3.
J Ethnopharmacol ; 247: 112269, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31610261

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Cisplatin (CP) is the classical chemotherapeutic drug for various cancer, but it also accompanies reproductive toxicity and genotoxicity. Liuwei Dihuang Pill (LW) is the traditional Chinese medicine prescription for treating Kidney-Yin deficiency syndrome, which has been reported to prevent and treat various diseases. However, the protective effect of LW on CP-induced reproductive toxicity and genotoxicity has not been reported. AIM OF THE STUDY: To investigate the potential protective effect and mechanism of LW on CP-induced reproductive toxicity and genotoxicity in male mice. MATERIALS AND METHODS: Mice were given LW (0.4, 1.2 and 3.6 g/kg) or Vitamin C (0.1 g/kg) once daily by oral gavage for thirteen consecutive days. Then, CP (3.00 mg/kg) was given intraperitoneal injection once daily for five consecutive days starting on the ninth day. The protective effects of LW against CP-induced reproductive toxicity and genotoxicity were evaluated by body weight, testis ratio, sperm count, sperm viability, sperm abnormal morphology type, micronuclei test, testicular histopathology, serum malondialdehyde (MDA), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) level. RESULTS: The results demonstrated that LW could significantly increase CP-induced the reduction of sperm count and sperm viability, then decrease abnormal sperm type rate and micronucleus rate. Moreover, LW also could improve testicular abnormal histopathologic morphology induced by CP exposure. Meanwhile, LW decreased serum MDA level and increased T-SOD, GSH-Px and CAT level compared to CP group. CONCLUSION: our findings show that LW has protective effects on CP-induced reproductive toxicity and genotoxicity. LW decreases serum MDA level and increases T-SOD, GSH-Px and CAT level, which indicates that antioxidant activity may be the potential mechanism of LW to resist reproductive toxicity and genotoxicity.

4.
J Ethnopharmacol ; 248: 112318, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-31629860

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Xiaochaihutang (XCHT) is a traditional Chinese medicine prescription for thousand years in China. Our previous researches show that XCHT has antidepressant-like effects in several depression models, but effect and mechanism of XCHT in perimenopausal depression are still vague. AIM OF THE STUDY: To reveal the antidepressant-like effect and mechanism of XCHT in perimenopausal mice. MATERIALS AND METHODS: Perimenopausal depression model is executed by ovariectomy combined with chronic unpredictable mild stress (OVX-CUMS). Tail suspension test (TST), forced swim test (FST), elevated plus-maze (EPM), novelty suppressed feeding (NSF) and locomotor activity are used to assess antidepressant-like effects of XCHT. The Level of estradiol (E2), follicle-stimulating hormone (FSH), gonadotrophin releasing hormone (GnRH), corticosterone (CORT), adrenocorticotrophic hormone (ACTH) and corticotropin releasing hormone (CRH) are evaluated by ELISA. Antidepressant mechanisms of XCHT in OVX-CUMS mice are analyzed by 5-hydroxytryptamine (5-HT), tryptophan hydroxylase 2 (TPH2) and estrogen receptor α and ß (ERα/ß). RESULTS: The results show that OVX-CUMS significantly increases the immobility time in TST and FST, increases latency to feed, decreases food consumption in NSF and both the time spend and number of entries in open arms in EPM. While, oral administration of XCHT can significantly normalize above depression-like behaviors in OVX-CUMS mice. Moreover, XCHT also remarkably normalized levels of 5-HT, 5-HIAA, E2, GnRH, CORT, ACTH and CRH in OVX-CUMS mice. Finally, the expression of ERß and TPH2 are decreased by OVX-CUMS in prefrontal cortex and hypothalamus, and XCHT can restore these decrease. CONCLUSION: Current findings suggest XCHT can alleviate perimenopausal depression-like behaviors, restore 5-HT and hormones in OVX-CUMS mice, which may be related to normalizing the functions of HPA/HPO axis and enhancing expression of ERß and TPH2 in prefrontal cortex and hypothalamus.

5.
Oncogene ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31801973

RESUMO

PINCH-1 is a cytoplasmic component of the cell-extracellular matrix (ECM) adhesion machine that is frequently overexpressed in cancer. The functions and mechanism of PINCH-1 in cancer, however, remain to be determined. Here, we show that PINCH-1 interacts with myoferlin, a transmembrane protein that is critical for cancer progression. High expression of both PINCH-1 and myoferlin correlates with poor clinical outcome in human breast cancer patients. Ablation of PINCH-1 from breast cancer cells diminished myoferlin level and suppressed breast cancer cell proliferation, migration, and endothelial cell tube formation in vitro and breast tumor growth, angiogenesis and metastasis in vivo. Mechanistically, PINCH-1 controls myoferlin level through its interaction with myoferlin and regulation of its ubiquitination and proteasome-dependent degradation. Functionally, re-expression of PINCH-1, but not that of a myoferlin-binding defectiveΔLIM2 mutant, effectively reversed the inhibition of myoferlin expression and breast cancer progression induced by loss of PINCH-1. Finally, restoration of myoferlin expression was sufficient to reverse PINCH-1-deficiency induced inhibition on breast cancer progression. These results reveal a PINCH-1-myoferlin signaling axis that is critical for breast cancer progression and suggest a new strategy for therapeutic control of breast cancer.

6.
Cell Rep ; 29(8): 2489-2504.e4, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31747615

RESUMO

Hair follicle stem cells (HFSCs) and subsequent generations of matrix progeny make lineage choices by responding to spatiotemporal signals; however, the cues driving that specification are not well understood. Here, we demonstrate that the dynamics of microRNA (miR)-29 expression are inversely proportional to HFSC lineage progression. Furthermore, we show that sustained miR-29a/b1 overexpression in anagen or telogen in mice causes a short-hair phenotype and eventual hair loss by inhibiting the proliferation of HFSCs and matrix cells and likely preventing their differentiation. Conversely, in a loss-of-function in vivo model, miR-29a/b1 deficiency accelerates HFSC lineage progression in telogen. Mechanistically, miR-29a/b1 blocks HFSC lineage specification by spatiotemporally targeting Ctnnb1, Lrp6, Bmpr1a, and Ccna2. We further show that skin-specific Lrp6 or Bmpr1a ablation partially accounts for the short-hair phenotype. Overall, these synergistic targets reveal miR-29a/b1 as a high-fidelity antagonist of HFSC lineage progression and a potential therapeutic target for hair loss.

7.
J Cell Biol ; 218(11): 3773-3794, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31578224

RESUMO

Mechano-environment plays multiple critical roles in the control of mesenchymal stem cell (MSC) fate decision, but the underlying signaling mechanisms remain undefined. We report here a signaling axis consisting of PINCH-1, SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), and bone morphogenetic protein type 2 receptor (BMPR2) that links mechano-environment to MSC fate decision. PINCH-1 interacts with Smurf1, which inhibits the latter from interacting with BMPR2 and consequently suppresses BMPR2 degradation, resulting in augmented BMP signaling and MSC osteogenic differentiation (OD). Extracellular matrix (ECM) stiffening increases PINCH-1 level and consequently activates this signaling axis. Depletion of PINCH-1 blocks stiff ECM-induced BMP signaling and OD, whereas overexpression of PINCH-1 overrides signals from soft ECM and promotes OD. Finally, perturbation of either Smurf1 or BMPR2 expression is sufficient to block the effects of PINCH-1 on BMP signaling and MSC fate decision. Our findings delineate a key signaling mechanism through which mechano-environment controls BMPR2 level and MSC fate decision.

8.
Opt Express ; 27(14): 19156-19167, 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31503679

RESUMO

In this paper, we propose a nonlinear Tomlinson-Harashima pre-coding (THP) scheme for nonlinear distortion suppression in direct-detected double sideband (DSB) PAM-4 transmission systems. Based on the traditional THP, the feedback term is modified by introducing nonlinear components. In this way, more accurate feedback can be obtained to mitigate the signal distortions, especially the nonlinear distortions including the signal-to-signal beating interference and nonlinear power series caused by chromatic dispersion and square-law detection. Meanwhile, we also propose to only reserve the nonlinear kernels with adjacent tap products in nonlinear THP, for the purpose of computation complexity reduction. To verify the effectiveness, transmissions of double sideband (DSB) PAM-4 signal in 1550nm window are experimentally demonstrated. Volterra FFE is adopted on the receiver side to suppress linear and nonlinear pre-cursors. We optimize various parameters of hardware and apply appropriate simplification to the nonlinear THP kernels. The results indicate that, the proposed nonlinear THP can lead to up to three folds BER reduction, compared to the conventional linear THP. Finally, with the combination of proposed nonlinear THP and conventional Volterra FFE, we successfully transmit 84-Gbps PAM-4 and 107-Gbps PAM-4 respectively over 80 km and 40 km under the hard decision forward error correction (HD-FEC) threshold of 3.8 × 10-3.

9.
Front Neurosci ; 13: 834, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31440134

RESUMO

Baicalin, the main active flavonoid constituent of Scutellaria baicalensis Georgi, has been reported to exert antidepressant effects. Hypothalamic-pituitary-adrenal (HPA) axis plays important roles in depression. However, antidepressant effect and mechanism of baicalin on HPA axis in hypothalamus are still unknown. In present study, we find baicalin significantly attenuates the increase of immobility time in tail suspension and forced swimming, improves the decrease of spending time in open arms, and restores the aberrant negative feedback of HPA axis in chronic corticosterone (CORT)-induced depressed mice. Moreover, proteomics finds 370 differentially expressed proteins after baicalin treatment, including 114 up-regulation and 256 down-regulation in hypothalamus. Systems biology analysis indicates the functions of differentially expressed proteins focus on phosphoserine binding and phosphorylation, especially participate in GR signaling pathway. Finally, our findings demonstrate that baicalin normalizes hypothalamic GR nuclear translocation via reducing GR phosphorylation to remodel negative feedback of HPA axis in CORT-induced mice.

10.
Psychol Aging ; 34(6): 780-790, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31380666

RESUMO

An influential account of normative aging effects on reading holds that older adults make greater use of contextual predictability to facilitate word identification. However, supporting evidence is scarce. Accordingly, we used measures of eye movements to experimentally investigate age differences in word predictability effects in Chinese reading, as this nonalphabetic language has characteristics that may promote such effects. Word-skipping rates were higher and reading times lower for more highly predictable words for both age groups. Effects of word predictability on word skipping did not differ across the 2 adult age groups. However, word predictability effects in reading time measures sensitive to both lexical identification (i.e., gaze duration) and contextual integration (i.e., regression-path reading times) were larger for the older than younger adults. Our findings therefore reveal that older Chinese readers make greater use of a word's predictability to facilitate both its lexical identification and integration with the prior sentence context. (PsycINFO Database Record (c) 2019 APA, all rights reserved).


Assuntos
Envelhecimento/fisiologia , Atenção/fisiologia , Envelhecimento Cognitivo/psicologia , Movimentos Oculares/fisiologia , Linguagem , Leitura , Adolescente , Idoso , Idoso de 80 Anos ou mais , Envelhecimento/psicologia , Grupo com Ancestrais do Continente Asiático , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Percepção Visual/fisiologia , Adulto Jovem
11.
Sci Total Environ ; 685: 1284-1293, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31300167

RESUMO

An upflow biofilm reactor was operated for 211 days to investigate the effects of vibration on anammox treatment performance. With vibration, the highest nitrogen removal rates (20 kg-N·m-3·d-1) were obtained on day 180. Since the vibration could directly applied on the biofilm, it could release the dinitrogen gas accumulated in the biofilm timely and reduce the internal mass transfer resistance sharply. The specific anammox activity increased by more than 3 times with a higher vibration intensity. Meanwhile, the unique random motion caused by mechanical vibration promotes the production of extracellular proteins. Moreover, the VSS reached 20.97 g·L-1 which was 1.6 times higher than the control reactor. Such enrichment method resulted in a hard and thick anammox biofilm with a special granular morphology, and the nitrite tolerance concentration could reach 500 mg-N·L-1. Operated with an adequate vibration intensity could maintain the biofilm thickness and conducive to improve the stability of the reactor. In addition, this technique also allowed the microorganisms inside the biofilm and those on the surface to reach the same culture conditions. Base on the batch experiments, intermittent vibration caused a decrease in energy consumption from about 7.757 (kW·h)·(kg-N)-1 in group 0-Lv7(60-60) to 0.912 (kW·h)·(kg-N)-1 in group 0-Lv7(5-60). Compared to the internal recycle without vibration, the energy consumption fell by a slice over 65%. Furthermore, the high-throughput sequencing results showed that the relative abundance of Candidatus Kuenenia in reactor 1 increased from 13.2% to 43.9%.


Assuntos
Biofilmes , Reatores Biológicos , Eliminação de Resíduos Líquidos/métodos , Compostos de Amônio/metabolismo , Vibração
12.
J Ethnopharmacol ; 243: 112079, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31302206

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Zhi-Zi-Hou-Po decoction (ZZHPD), a classical Chinese prescription, has been reported to improve depressive behaviors in clinic. However, definite pharmacological effects and mechanisms of ZZHPD on monoaminergic system and hippocampal neurogenesis are ambiguous. It need to be further illuminated. AIM OF THE STUDY: Our study is designed to reveal pharmacological mechanisms of ZZHPD on depression through pharmacokinetics, monoamine neurotransmitters and neurogenesis. MATERIALS AND METHODS: Chronic unpredictable mild stress (CUMS) is used to establish rats model of depression. Then, the antidepressant effects of ZZHPD are evaluated by detecting body weight, sucrose preference and forced swimming test. The regulatory functions of ZZHPD on monoaminergic system are assessed by measuring monoamine neurotransmitters, neurotransmitter precursor substances, synthesized rate-limiting enzymes and transporters. Finally, potential molecular mechanism of ZZHPD on hippocampal neurogenesis is evaluated by investigating newborn immature neuron and newborn mature neuron. RESULTS: Our results show that ZZHPD remarkably normalizes CUMS-induced decline in weight gain, decrease of sucrose consumption rate in sucrose preference test and increase of immobility time in forced swimming test. Moreover, ZZHPD significantly reverses CUMS-induced reduction of 5-hydroxytryptamine (5-HT), dopamine (DA), tryptophan (Trp), tyrosine (Tyr), tryptophan hydroxylase2 (TPH2) and tyrosine hydroxylase (TH), whereas decreases level of serotonin transporter (SERT) in CUMS-induced rats. Finally, ZZHPD obviously improves CUMS-induced decrease of newborn immature neuron and newborn mature neuron in dentate gyrus of hippocampus. CONCLUSION: This study demonstrates that ZZHPD can alleviate CUMS-induced depression-like behaviors. It is probably attributed to the fact that ZZHPD could enhance monoaminergic system and hippocampal neurogenesis. Our findings provide the new perspectives on molecular targets of ZZHPD, and it will facilitate its clinical application.

13.
Opt Express ; 27(13): 18883-18891, 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31252823

RESUMO

We present a pulse linearly polarized diode-pumped cesium-vapor laser (Cs-DPAL) based on an acousto-optical modulator (AOM) for the first time. The continuous wave (CW) performance of the Cs-DPAL was first investigated, and ~1.05 W linearly polarized CW laser was obtained. Next, we applied a rectangular signal to modulate the AOM. The Cs-DPAL realized a pulse laser output with a maximum repetition rate of 1 MHz and minimum pulse duration of 238 ns. To the best of our knowledge, this is the highest repetition rate reported thus far for a diode-pumped alkali-vapor laser (DPAL). The maximum output power of the pulse laser reached ~0.20 W, and the corresponding Mx 2 and My 2 factors were 1.31 and 1.19, respectively. Finally, we realized code modulation of the Cs-DPAL, with a maximum bit rate of 2 Mb/s.

14.
Sci Total Environ ; 688: 505-512, 2019 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-31254816

RESUMO

A new type of biological folded non-aerated filter (BFNAF) was found to be superior and feasible for the treatment of NH4+-N wastewater. It was constructed with the folded structure suitable for the nylon biomass carrier. The advantages of the BFNAF included low energy consumption, long reaction path, large biofilm surface area and non-clogging compared to the traditional biological aerated filter. In this study, the effects of hydraulic retention time (HRT), and the influent NH4+-N concentration on the performance of BFNAF were investigated and optimized by the response surface methodology. Under the optimal operating condition (HRT, 10 h; NH4+-N concentration, 52 mg/L), the removal efficiency and removal rate were 94.62 ±â€¯0.63% and 0.106 kg-NH4+ m-3 day-1, respectively. The results showed that the BFNAF reactor could remove NH4+-N from wastewater and realized the nitrification process effectively under natural ventilation conditions.


Assuntos
Compostos de Amônio/análise , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/análise , Biofilmes , Reatores Biológicos , Nitrificação , Águas Residuárias
15.
Bioresour Technol ; 289: 121696, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31252319

RESUMO

A conventional biological filter has been shown to be a viable method for removing nitrogenous compounds from wastewater, but it still has many disadvantages. In this study, a biological folded non-aerated filter (BFNAF) was designed, and its feasibility for nitrogen-loaded wastewater treatment has been confirmed. Effects of the HRT and the COD/N ratio on the performance of BFNAF were investigated. Through response surface method, when the COD/N ratio and the HRT were 5.39 and 10.83 h, removal efficiencies of NH4+, COD and TN reached maximum values of 88.62 ±â€¯0.81%, 76.12 ±â€¯0.57%, and 50.48 ±â€¯1.02%, respectively. In addition, it was found that several denitrifying bacteria, such as Azoarcus, Arcobacter, Flavobacterium, along with many ammonia-oxidizing bacteria and nitrite-oxidizing bacteria, co-existed in the community of the biofilm. All the results showed that the BFNAF could realize the simultaneous nitrification and denitrification (SND) process effectively.


Assuntos
Nitrogênio/isolamento & purificação , Águas Residuárias/química , Bactérias , Biofilmes , Desnitrificação , Filtração , Nitrificação
16.
BMC Cancer ; 19(1): 465, 2019 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-31101027

RESUMO

BACKGROUND: Gene variants are dependable and sensitive biomarkers for target-specific therapies in breast cancer (BC). However, detection of mutations within tissues has many limitations. Plasma circulating free DNA (cfDNA) has been reported in many studies as an alternative tool for detection of mutations. But the diagnostic accuracy of cfDNA for most mutations in BC needs to be reviewed. This study was designed to perform comparative assessment of the diagnostic performance of cfDNA and DNA extracted from tissues for detection of single nucleotide variants (SNV) and copy number variants (CNV). METHODS: True-positive (TP), false-positive (FP), false-negative (FN), and true-negative (TN) values were extracted from each selected study. Pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated. Subgroup analysis and single study omitted analysis were performed to quantify and explain the study heterogeneity. RESULTS: Twenty eligible studies that involved 1055 cases were included in this meta-analysis. SNV studies in early breast cancer (EBC) subgroup are not suitable for meta-analysis owing to high heterogeneity. However, in advanced breast cancer (ABC) subgroup, the pooled sensitivity and specificity of detection of SNVs were 0.78 (0.71-0.84) and 0.92 (0.87-0.95), respectively. The summary receiver operative curve (SROC) exhibited an area under the curve (AUC) of 0.91(0.88-0.93). The pooled results of studies involving subgroups of PIK3CA, TP53, and ESR1 indicate that the diagnostic value of different genes is different, such as AUC for PIK3CA and TP53 were reported to be 0.96 (0.94-0.98) and 0.94 (0.91-0.95), respectively, and ESR1 had the lowest diagnostic value of 0.80 (0.76-0.83). Owing to the low sensitivity and AUC in the cases of CNV, there is no value for cfDNA-based detection of CNV based on insufficient amount of CNV data. CONCLUSION: This meta-analysis suggests that the detection of gene mutations in cfDNA have adequate diagnostic accuracy and can be used as an alternative to the tumor tissue for detection of SNV but not for CNV in BC yet.


Assuntos
Neoplasias da Mama/genética , Ácidos Nucleicos Livres/sangue , Variações do Número de Cópias de DNA , Análise Mutacional de DNA/métodos , DNA de Neoplasias/sangue , Polimorfismo de Nucleotídeo Único , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/sangue , Neoplasias da Mama/sangue , Confiabilidade dos Dados , Feminino , Humanos , Pessoa de Meia-Idade , Mutação , Sensibilidade e Especificidade
17.
Cells ; 8(5)2019 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-31096686

RESUMO

Ullrich congenital muscular dystrophy (UCMD) bring heavy burden to patients' families and society. Because the incidence of this disease is very low, studies in patients are extremely limited. Animal models of this disease are indispensable. UCMD belongs to extracellular matrix-related diseases. However, the disease models constructed by knocking out some pathogenic genes of human, such as the Col6a1, Col6a2, or Col6a3 gene, of mice could not mimic UCMD. The purpose of this study is to construct a mouse model which can resemble the pathology of UCMD. miR-29 is closely related to extracellular matrix deposition of tissues and organs. To address this issue, we developed a mouse model for overexpression miR-29 using Tet-on system. In the muscle-specific miR-29ab1 cluster transgenic mice model, we found that mice exhibited dyskinesia, dyspnea, and spinal anomaly. The skeletal muscle was damaged and regenerated. At the same time, we clarify the molecular mechanism of the role of miR-29 in this process. Different from human, Col4a1 and Col4a2, target genes of miR-29, are the key pathogenic genes associating with these phenotypes. This mouse model simulates the human clinical and pathological characteristics of UCMD patients and is helpful for the subsequent research and treatment of UCMD.


Assuntos
Modelos Animais de Doenças , Camundongos , MicroRNAs/genética , Distrofias Musculares/genética , Distrofias Musculares/patologia , Esclerose/genética , Esclerose/patologia , Animais , Colágeno Tipo IV/genética , Humanos , Camundongos Endogâmicos C57BL , Músculo Esquelético/patologia , Mutação , Fragmentos de Peptídeos/genética , Fenótipo
18.
Front Psychol ; 10: 721, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31001175

RESUMO

Inhibition of Return (IOR) refers to an individual's slowed localization or discrimination performance for targets that appear in previously cued versus uncued location after a relatively long delay after cue (∼300-500 ms). The current study adopted a cue-target paradigm and used behavioral and event-related potential (ERP) measures to investigate whether IOR would be modulated by emotional faces during an emotion recognition task. For reaction time measure, we found IOR effect and the magnitude of IOR effect were comparable for fearful face target and neutral face target. For ERP measures, valid cues were associated with smaller P1 and larger N1 waveform than that for invalid cues. Fearful faces were associated with a larger N170 than neutral faces. The onset latency of the stimulus-locked lateralised readiness potential (LRP) in the valid cue condition was longer than that in the invalid cue condition, while there was no significant difference on the onset latency of the response-locked LRP between the valid cue and invalid cue condition. These results support the notion that, regardless the emotion component of the stimulus, the inhibitory bias of attention to previous visited location before response contributes to the IOR.

20.
Nat Prod Res ; : 1-6, 2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-30931644

RESUMO

Two new compounds, namely 6α-hydroxy-14-taraxerene-3,16,21-trione and 6α,26-dihydroxy-14-taraxerene-3,16,21-trione were isolated from the Ledum palustre L. Their chemical structures were confirmed after a combined analysis of IR, HR-ESI-TOFMS, 1D-NMR, and 2D-NMR. The compounds were evaluated for cell growth inhibitory activity against two cancer cell lines.

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