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1.
Anal Chim Acta ; 1144: 34-42, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33453795

RESUMO

Herein, we report our strategy to develop the efficient chemosensor and real-time monitoring technique for cyanuric chloride (TCT) detection. A luminescent macrocyclic mononuclear Sm(III) complex Sm-2k bearing with two dynamic imine bonds has been constructed via the template synthesis between dialdehyde H2Qk and matched diamine 1,2-bis(2-aminoethoxy)ethane. Sensing experiments reveal that complex Sm-2k exhibits the turn-off fluorescent and colorimetric response for TCT in CH3OH. It is especially encouraging that this optical sensing process is not only rapid within 60 s but also high-efficient in the presence of TCT analogues as well as sensitive with the low limit of detection (LOD, 1.74 µM) and wide linear sensing range. Mechanism studies demonstrate that TCT sensing is mainly based on the imine bond transformation of probe Sm-2k, which is due to the increased acidity induced by TCT. Meanwhile, a smartphone-based analytical method was developed to make complex Sm-2k accessible for the real-time TCT detection by RGB value outputs. It is believed that this work can shed some constructive lights on design of chemosensors and convenient detection technique for highly reactive analytes.

2.
J Geriatr Cardiol ; 17(10): 597-603, 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33224178

RESUMO

Background: Heart failure (HF) is a leading cause of hospitalization and mortality for older chronic kidney disease (CKD) patients. However, the epidemiological data is scarce. We aimed to determine the prevalence of left ventricular (LV) dysfunction and HF, and to explore the risk factors for HF among those patients. Methods: This is a cross-sectional analysis of the China Hypertension Survey conducted between October 2012 and December 2015. A total of 5, 808 participants aged ≥ 65 years were included in the analysis. Self-reported history of HF and any other cardiovascular diseases was acquired. 2-D and Doppler echocardiography were used to assess LV dysfunction. CKD was defined as either estimated glomerular filtration rate (eGFR) < 60 mL/min per 1.73 m2 or urinary albumin to creatinine ratio (ACR) ≥ 30 mg/g. Results: Among CKD patients aged ≥ 65 years, the weighted prevalence of HF, heart failure with preserved ejection fraction (HFpEF), heart failure with mid-range ejection fraction (HFmrEF), and heart failure with reduced ejection fraction (HFrEF) was 4.8%, 2.5%, 0.8%, and 1.7%, respectively. The weighted prevalence of HF was 5.0% in patients with eGFR < 60 mL/min per 1.73 m2, and was 5.9% in patients with ACR ≥ 30 mg/g. The prevalence of LV systolic dysfunction was 3.1%, and while it was 8.9% for moderate/severe diastolic dysfunction. Multivariate analysis showed that smoking was significantly associated with the risk of HF. Furthermore, age, smoking, and residents in rural areas were significantly associated with a risk of LV diastolic dysfunction. Conclusions: The prevalence of HF and LV dysfunction was high in older patients with CKD, suggesting that particular strategies will be required.

3.
Front Genet ; 11: 779, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32849791

RESUMO

Long non-coding RNAs (lncRNAs) may be a regulatory factor of tumorigenesis. However, it is unclear what its biomechanisms are in breast cancer. In this study, different lncRNAs were detected in breast cancer through microarray analysis (GSE119233) and LINC01705 was selected for further study. qRT-PCR was then utilized for the detection of LINC01705 expression in breast cancer cells. A transwell assay, flow cytometry, 5-ethynyl-2'-deoxyuridine (EdU), a cell counting Kit-8 (CCK-8), and a wound-healing assay were performed to determine cell migration, invasion, apoptosis, and proliferation in breast cancer, respectively. For the identification of potential targets of LINC01705, dual-luciferase reporter gene and bioinformatics assays were conducted. Moreover, for the clarification of their interaction and roles in the regulation of the occurrence of breast cancer, Western blotting and RIP assays were conducted. Our findings revealed high LINC01705 expression in breast cancer tissues relative to adjacent non-cancerous tissues (n = 40, P < 0.001). Overexpression of LINC01705 notably enhanced cell migration and proliferation in breast cancer. In addition, LINC01705 positively regulated the translocated promoter region, nuclear basket protein (TPR) through competition with miR-186-5p. In conclusion, our results suggest that LINC01705 is implicated in the progression of breast cancer via competitively binding to miR-186-5p as a competing endogenous RNA (ceRNA), thereby regulating TPR expression.

4.
ACS Omega ; 4(19): 18334-18341, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31720535

RESUMO

Here, we report our trials to regulate the luminescence performance of the macrocyclic samarium(III) complex and prepare four excellent luminescent Sm(III) complex-doped poly(methylmethacrylate) (PMMA) composites. Four 23-membered [1 + 1] Schiff-base macrocyclic mononuclear Sm(III) complexes, Sm-2 a -Sm-2 d , originating from dialdehydes with different pendant arms and 1,2-bis(2-aminoethoxy)ethane, have been constructed by the template method. Crystal structures reveal that every Sm(III) ion with the coordination geometry of a distorted bicapped square antiprism is capsulated by the macrocyclic cavity environment forming the "lasso-type" protection. Relative photophysical properties of macrocyclic Sm(III) complexes are carefully investigated in solid-state, methanol solution, and doped PMMA film, and all these show characteristic emissions of the Sm(III) ion associated with satisfactory lifetimes and quantum yields in all media, which could be comparable to reported outstanding examples. Especially, the luminescence performance for this type of Sm(III) complex could be regulated in the solid state by the use of different functional groups in the pendant arm while it is not achieved in solution and the doped PMMA composite. High emitting and air-stable plastic materials could be obtained when these Sm(III) complexes are doped in PMMA with 0.1 wt % mixing ratio, and the corresponding maximum lifetime and quantum yield are 61.2 µs and 0.63% in the case of complex Sm-2 a , respectively. We believe that these highly luminescent "lasso-type" Sm(III) complexes and doped PMMA composites are valuable references in the design of luminescent lanthanide(III) hybrid materials.

5.
Analyst ; 144(17): 5254-5260, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31364615

RESUMO

In this paper we report a novel probe based on a luminescent 23-membered [1 + 1] Schiff-base macrocyclic mononuclear Sm(iii) complex Sm-2e, originating from the dialdehyde H2Qe and 1,2-bis(2-aminoethoxy)ethane precursors, which is synthesized by the Sm(iii) ion template method. X-ray structural analyses confirm that each ten-coordinate Sm(iii) center with the coordination geometry of a distorted bicapped square antiprism is fully encapsulated by a flexible macrocyclic ligand H2L2e to form a "lasso-type" architecture, and this architecture could enable efficient energy transfer in various solvents confirmed by long lifetimes (33.5-65.2 µs) and high quantum yields (0.23-0.76%) of the Sm(iii) ion. Simultaneously, complex Sm-2e could serve as a probe for sensing organic solvents. Particularly, this complex probe Sm-2e exhibits a highly selective, rapid and sensitive response to tetrahydrofuran (THF), which is easily distinguished by a large absorption shift, even visible to the naked eye, and complete fluorescence quenching. Moreover, the limit of detection for THF is about 0.20% determined by titration experiments, and good selectivity for THF could still be realized in mixture solvents. Consequently, this colorimetric and "turn off" fluorescent probe Sm-2e could be a valuable candidate as a sensor material for sensing THF which has been rarely reported.


Assuntos
Complexos de Coordenação/química , Corantes Fluorescentes/química , Furanos/análise , Colorimetria/métodos , Complexos de Coordenação/síntese química , Fluorescência , Corantes Fluorescentes/síntese química , Limite de Detecção , Samário/química , Bases de Schiff/síntese química , Bases de Schiff/química , Espectrometria de Fluorescência/métodos
6.
Chin J Integr Med ; 23(12): 908-915, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27145942

RESUMO

OBJECTIVE: To explore the protective effects of Tibetan medicine Zuo-Mu-A Decoction (, ZMAD) on the blood parameters and myocardium of high altitude polycythemia (HAPC) model rats. METHODS: Forty male Wistar rats were randomly divided into 4 groups by a random number table, including the normal, model, Rhodiola rosea L. (RRL) and ZMAD groups (10 in each group). Every group was raised in Lhasa to create a HAPC model except the normal group. After modeling, rats in the RRL and the ZMAD groups were administered intragastrically with RRL (20 mL/kg) and ZMAD (7.5 mL/kg) once a day for 2 months, respectively; for the normal and the model groups, 5 mL of distilled water was administered intragastrically instead of decoction. Then routine blood and hematologic rheology parameters were taken, levels of erythropoietin and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were tested, and ultrastructural change in the left ventricular myocardium was observed using transmission electron microscopy. RESULTS: Compared with the model group, ZMAD significantly reduced the red blood cell count, hemoglobin levels, whole blood viscosity at low/middle shear rates, plasma viscosity, erythrocyte electrophoretic time, erythropoietin and 8-OHdG levels, and also increased the erythrocyte deformation index (P<0.05). There was no difference in all results between the RRL and the ZMAD groups. The cardiac muscle fibers were well-protected, mitochondrial matrix swelled mildly and ultrastructure changes were less prominent in the ZMAD group compared with the model group. CONCLUSION: ZMAD has significant protective effects on the blood parameters against HAPC, and also has the beneficial effect in protecting against myocardial injury.


Assuntos
Doença da Altitude/sangue , Doença da Altitude/tratamento farmacológico , Medicina Tradicional Tibetana , Miocárdio/patologia , Policitemia/sangue , Policitemia/tratamento farmacológico , Substâncias Protetoras/uso terapêutico , 8-Hidroxi-2'-Desoxiguanosina , Doença da Altitude/complicações , Animais , Desoxiguanosina/análogos & derivados , Desoxiguanosina/sangue , Modelos Animais de Doenças , Eritropoetina/sangue , Miocárdio/ultraestrutura , Policitemia/complicações , Substâncias Protetoras/farmacologia , Reologia/efeitos dos fármacos
7.
J Biochem ; 149(6): 673-84, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21292804

RESUMO

Three-dimensional structural model of epoxide hydrolase (PchEHA) from Phanerochaete chrysosporium was constructed based on X-ray structure of Agrobacterium radiobacter AD1 sEH using SWISS-MODEL server. Conserved residues constituting the active site cavity were identified, of which the functional roles of 14 residues were determined by site-directed mutagenesis. In catalytic triad, Asp105 and His308 play a leading role in alkylation and hydrolysis steps, respectively. Distance between Asp105 and epoxide ring of substrate may determine the regiospecificity in the substrate docking model. Asp277 located at the entrance of substrate tunnel is concerned with catalysis but not essential. D307E had the highest activity and lower enantioselectivity among 14 mutants, suggesting Asp307 may be involved in choice of substrate configuration. Y159F and Y241F almost exhibited no activity, indicating that they are essential to bind substrate and facilitate opening of epoxide ring. Besides, His35-Gly36-Asn37-Pro38, Trp106 and Trp309 surrounding Asp105, may coordinate the integration of active site cavity and influence substrate binding. Especially, W106I reversed the enantioselectivity, perhaps due to more deteriorative impact on the preferred (R)-styrene oxide. Gly65 and Gly67 occurring at ß-turns and Gly36 are vital in holding protein conformation. Conclusively, single conserved residue around the active sites has an important impact on catalytic properties.


Assuntos
Epóxido Hidrolases/genética , Mutagênese Sítio-Dirigida , Phanerochaete/enzimologia , Homologia Estrutural de Proteína , Cristalografia por Raios X , Epóxido Hidrolases/química , Epóxido Hidrolases/metabolismo , Modelos Moleculares , Estrutura Terciária de Proteína
8.
Zhonghua Liu Xing Bing Xue Za Zhi ; 31(1): 1-4, 2010 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-20302687

RESUMO

OBJECTIVE: To determine the effects related to community-based standardized blood pressure management programs on the control of hypertension. METHODS: A protocol of community-based standardized blood pressure management was developed based on the current Chinese guideline for prevention, treatment of hypertension. Grass-roots caretakers from community health service centers across China were trained using this protocol and required to manage hypertensive patients according to the protocol. Patients were treated on therapeutic life style change or/and medication, and followed up based on the criteria of risk stratification. The control rate of hypertension was evaluated after 1 year. Effect of intervention (EI) was estimated as '1 year rate (mean)' minus the number showed at the baseline. RESULTS: By the end of 2008, a total of 29 411 hypertensive patients (47.2% for male, mean age 61.4 +/- 10.9 years) with full information had been under management for one year according to the protocol. Among all patients, 8.9% were classified as under low risk, 50.8% as moderate risk and 40.3% as high and very high risk showed in baseline data. After standardized management, the EI of smoking, drinking and systolic/diastolic blood pressure were -7.1% (P < 0.05), -7.3% (P < 0.05), and -14.8-8.3 mm Hg (P < 0.05), respectively. However, EI of overweight/obesity was 0.3% (P > 0.05). For all patients, the control rate rose to 74.7%, with EI as 53.1%, and all of the sub-groups, including age, risk stratification, had significant increases. The longer the management was under, the higher the control rate was seen. Results from the multivariate logistic regression showed that older age, male and having higher blood level were adverse factors for the undertaking the control and management programs of hypertension. CONCLUSION: Results from our study showed that standardized management could significantly improve the program on the control of hypertension at the community level, in China.


Assuntos
Serviços de Saúde Comunitária , Avaliação do Impacto na Saúde , Hipertensão/prevenção & controle , Idoso , Gerenciamento Clínico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
9.
Biochimie ; 92(7): 779-88, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20188787

RESUMO

A putative gene encoding proline iminopeptidase (PchPiPA) was cloned from Phanerochaete chrysosporium BKM-F-1767 by RT-PCR and expressed successfully in Escherichia coli. The cDNA is 942 bp in length and encodes 313 amino acids. The recombinant enzyme was only able to hydrolyze Pro-pNA among the tested synthetic substrates. There is no activity detected toward Leu-pNA, Phe-pNA and Tyr-pNA, as well as GGG-pNA, SGR-pNA, AAV-pNA, AAPL-pNA, AAVA-pNA. And the recombinant enzyme could cleave the peptides derived from enzyme-hydrolytic natural proteins to release free lysine, which was confirmed using synthetic oligopeptides with lysine at N termini as substrate. The optimal pH and temperature for this enzyme were 8.0 and 45 degrees C, respectively. The catalytic activity was inhibited slightly by Mg(2+), Al(3+), Ca(2+), Fe(3+), Fe(2+) and Ba(2+); strongly by Ni(2+), Mn(2+) and Co(2+), and almost inactivated by Zn(2+), Cu(2+) and Hg(2+). In addition, the enzyme was not sensitive to EDTA-Na(2), as well as redoxes of DTT, beta-ME and H(2)O(2). The protease inhibitors of benzamidine hydrochloride and phenylmethyl sulfonyfluoride caused a moderate inhibition. The V(max), K(m) and k(cat) toward Pro-pNA were 347.86 mumol min(-1) mg(-1), 2.15 mM and 218.10 S(-1), respectively. The deduced catalytic triad of Ser(107), Asp(264) and His(292) was confirmed by site-directed mutagenesis because the individual replacement of Ser(107) to Asp, Asp(264) to Ala or His(292) to Leu led complete inactivation. Transcriptional analysis by RT-PCR showed that PchPiPA could be expressed under ligninolytic and non-ligninolytic conditions. Conclusively, it was suggested that the proline iminopeptidase may be a member of the proteolytic system in this fungus. The availability of recombinant protein may be potentially used in certain proteolytic processing.


Assuntos
Aminopeptidases/genética , Aminopeptidases/metabolismo , Phanerochaete/enzimologia , Sequência de Aminoácidos , Aminopeptidases/química , Aminopeptidases/isolamento & purificação , Biocatálise , Clonagem Molecular , Estabilidade Enzimática , Manipulação de Alimentos , Concentração de Íons de Hidrogênio , Hidrólise/efeitos dos fármacos , Cinética , Metais/farmacologia , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Peptídeos/metabolismo , Phanerochaete/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Especificidade por Substrato , Temperatura , Transcrição Genética
11.
Zhonghua Xin Xue Guan Bing Za Zhi ; 35(8): 761-4, 2007 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-17963640

RESUMO

OBJECTIVE: To evaluate the applicability of developed prediction models of ischemic cardiovascular diseases (ICVD) in Chinese to other Chinese populations. METHOD: We used the independent prospective cohort established in early 1990's from China Multi-Center Collaborative Study of Cardiovascular Epidemiology (MUCA), as the validation cohort, to test the hypothesis. The area under ROC curve (AUC) based on the application of the Cox optimal model and the simplified model to the validation cohort were calculated and to test the ability of the prediction models to discriminate events from nonevents. Applicability was evaluated by comparing the mean probability estimates in each decile of probability in the validation cohort with the observed incidence with the Hosmer-Lemeshow test. RESULTS: The validation cohort enrolled a total of 17 329 men and women aged 35 to 59 years baseline 1992 - 1994. In this paper, we used data from the remaining 15 100 participants after excluding 2229 subjects for at missing value of risk factors. During 11-year follow up of the cohort, there were 347 ICVD events (206 for men and 141 for women), including 83 coronary heart disease events (56 men and 27 women) and 268 ischemic strokes (154 men and 114 women). ROC curves for men and women showed good and almost identical discrimination for optimal model (the AUCs (95% CI) were 0.796 (0.762 - 0.829) for men and 0.791 (0.755 - 0.828) for women), simplified model (the AUCs (95% CI) were 0.792 (0.758 - 0.825) for men and 0.783 (0.746 - 0.821) for women) and score system (the AUCs (95% CI) were 0.791 (0.757 - 0.825) for men and 0.779 (0.741 - 0.817) for women) in the validation cohort. The predicted 10-year risk of ICVD by optimal models and observed incidence of ICVD in the validation cohort in each decile were compared. Hosmer-Lemeshow chi2 was 3.7 for men (P = 0.879) and 27.7 for women (P < 0.001). Whereas the largest difference between the observed rate and the predicted rate was only 1%. CONCLUSIONS: The prediction models for estimating 10-year risk of ICVD had satisfied predictive capability when they were applied to the validation cohort and are applicable to other Chinese populations.


Assuntos
Doenças Cardiovasculares/epidemiologia , Isquemia/epidemiologia , Modelos Cardiovasculares , Adulto , Área Sob a Curva , Isquemia Encefálica/epidemiologia , Isquemia Encefálica/prevenção & controle , Doenças Cardiovasculares/prevenção & controle , China/epidemiologia , Estudos de Coortes , Feminino , Seguimentos , Humanos , Isquemia/prevenção & controle , Masculino , Pessoa de Meia-Idade , Curva ROC , Medição de Risco
12.
Zhonghua Liu Xing Bing Xue Za Zhi ; 28(11): 1060-3, 2007 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-18396655

RESUMO

OBJECTIVE: To assess the relationship between parental history and the incidence of stroke in Chinese populations. METHODS: A total of 15,131 individuals (7015 men and 8116 women, aged from 35 to 59 years) in China were enrolled and followed up from 1992 to 2005. Information on the parental history of stroke of the participants was collected at baseline study under face to face interview. Individuals were divided into three groups according to their parental history of hypertension: those with no parental history of hypertension (Group 1), those with only one parent having the history of hypertension (Group 2) and those with both parents having the history of hypertension (Group 3). The hazard ratio and its 95% confidence interval were calculated using the Cox proportional hazard model. RESULTS: There were 370 stroke events during 163 858 person-years of observation. After adjusting for age and sex, the hazard ratioon stroke for group 1, group 2 and group 3 were 1.00, 1.74 (1.33-2.29), 3.61 (1.86-7.01), respectively. After adjusting for age, sex, smoking, drinking, serum total cholesterol (TC), high density lipid cholesterol (HDL-C), fasting glucose, body mass index (BMI) and systolic blood pressure (SBP) in the model, the hazard ratio for stroke of those three groups were 1.00, 1.34 (1.02-1.77), 2.50 (1.29-4.87), respectively. CONCLUSION: Individuals with parental history of stroke had a higher risk of stroke, and this was especially true for those with both parents having the history of stroke.


Assuntos
Acidente Vascular Cerebral/epidemiologia , Adulto , China/epidemiologia , Estudos de Coortes , Feminino , Humanos , Hipertensão/epidemiologia , Masculino , Pessoa de Meia-Idade , Pais , Modelos de Riscos Proporcionais , Fatores de Risco
13.
Zhonghua Xin Xue Guan Bing Za Zhi ; 34(8): 747-51, 2006 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-17081405

RESUMO

OBJECTIVE: To assess the relationship between parental history of hypertension and the incidence of cardiovascular disease in Chinese populations. METHODS: A total of 15 131 individuals (7015 men and 8116 women, aged from 35 to 59 years) in China were enrolled and followed up from 1992 to 2005. The information on the parental history of hypertension of the participants was collected. The individuals were divided into three groups according to their parental history of hypertension: those without parental history of hypertension (group 1), those with one parent history of hypertension (group 2) and those with both parents history of hypertension (group 3). The hazard ratio and its 95% confidence interval were calculated using the Cox proportional hazard model. RESULTS: There were 448 cardiovascular events (including 82 cardiac events and 370 stroke events and 4 with both cardiac and stroke events) during the 163 858 person-years of observation. After adjusting for age, smoking and drinking, the hazard ratio for cardiovascular disease for group 1, group 2 and group 3 were 1.00, 1.34 (1.01 - 1.78), 2.58 (1.62 - 4.11) in men respectively and 1.00, 1.77 (1.27 - 2.45), 2.55 (1.44 - 4.54) in women respectively. After further adjusting for serum total cholesterol (TC), high density lipid cholesterol (HDL-C), fasting glucose, body mass index (BMI) and systolic blood pressure (SBP) in the model, the hazard ratio for cardiovascular disease for those three groups were 1.00, 1.01 (0.76 - 1.35), 1.72 (1.07 - 2.75) in men respectively and 1.00, 1.31 (0.94 - 1.84), 1.76 (0.98 - 3.15) in women respectively. CONCLUSION: The individuals with parental history of hypertension have a higher risk of cardiovascular disease, especially for those with both parents history of hypertension.


Assuntos
Doenças Cardiovasculares/epidemiologia , Hipertensão/epidemiologia , Adulto , Distribuição por Idade , Idoso , China/epidemiologia , Feminino , Seguimentos , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Pais , Estudos Prospectivos , Fatores de Risco , Amostragem
14.
Zhonghua Liu Xing Bing Xue Za Zhi ; 27(11): 930-3, 2006 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-17402190

RESUMO

OBJECTIVE: To examine the associations between physical activity measures [metabolic equivalents of energy expenditure (MET) per hour per day] and ischemic cardiovascular diseases (ICVD) in Chinese population. METHODS: A survey on cardiovascular risk factors was conducted in different areas of China in fall 1998. People aged 35 to 59 but without a history of coronary heart and stroke at baseline were prospectively followed and 11 849 subjects whose information were complete at the end of second follow-up were valid. Cox regression was used to estimate the hazard ratios (HRs) for incident ischemic cardiovascular diseases and the different measures of physical activity. RESULTS: During a mean follow-up period of 5.9 years, 84 incident ischemic cardiovascular events were ascertained. We examined the HRs of ischemic cardiovascular events for a 1-unit change in METs value, which were included in the models as continuous variable. There were negative association of METs values found with ischemic cardiovascular events in total, urban, rural, male and female subjects, and statistical significance in the urban (HRs = 0.22, 95% CI: 0.05-0.95) but the association was weakened after adjustment for demographic factors. When further adjustment for other intermediate factors, the significance in the urban was again attenuated. When the urban males and females, rural males and females were divided into 3 groups according to their respective tertiles and the combination of different population groups, the factors of male/female and urban/ rural were equally distributed in different groups, and no more adjustment in the Cox model. The multivariate - adjusted (age and education attainment) HRs associated with the tertiles, from lowest to highest, were: 1, 1.03 and 0.65 (P(trend) = 0.170) for the total, 1, 0.72 and 0.64 for the urban, 1, 1.49 and 0.72 for the rural, 1, 1.05 and 0.59 for men, 1, 0.90 and 0.84 for women. CONCLUSION: The totality of our findings pointed to METs per hour per day seemed to be weakly associated with a reduction in ischemic cardiovascular events incidence among urban middle-aged adults.


Assuntos
Metabolismo Energético , Isquemia Miocárdica/epidemiologia , China/epidemiologia , Feminino , Humanos , Masculino , Fatores de Risco , Saúde da População Rural , Saúde da População Urbana
15.
Zhonghua Xin Xue Guan Bing Za Zhi ; 33(9): 848-52, 2005 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-16266465

RESUMO

OBJECTIVE: To explore the relationship between nutrient intake and the incidence of hypertension in middle-aged Chinese. METHODS: A prospective study was conducted in 653 men and women of Beijing and Guanzhou, aged 35-59 years. The subjects had no hypertension or other cardiovascular diseases at baseline risk factor and dietary survey carried out in 1983-1984. The baseline information of nutrient intake was collected by using a 24-hour recall method on three consecutive days. The incidence of hypertension was determined through the follow-up survey in 1993-1994. The subjects were categorized into three groups according to tritiles of each baseline nutrient intake and the relative risk for hypertension incidence in each group was calculated by using the logistic regression model with the group of lowest risk as the referent. RESULTS: During the period of 1983-1984 to 1993-1994, 170 among 653 subjects had developed hypertension; 92 were men and 78 were women. After adjustment for other risk factors, the relative risk of hypertension for different protein intake groups was 1.0, 0.87 (95% CI 0.54-1.40) and 0.52 (95% CI 0.32-0.87), respectively, and the p value for trend analysis was 0.011; the relative risk of hypertension for different sodium intake groups was 1.0, 1.12 (95% CI 0.66-1.88) and 1.85 (95% CI 1.09-3.14), respectively, and the p value for trend analysis was 0.015. There was no significant relationship between the other nutrient intake and hypertension incidence. CONCLUSION: Among middle-aged Chinese, protein and sodium may be two of the most important dietary factors affecting the incidence of hypertension. Increasing protein intake and reducing sodium intake might be helpful for the prevention of hypertension.


Assuntos
Hipertensão/epidemiologia , Hipertensão/prevenção & controle , Adulto , China/epidemiologia , Ingestão de Energia , Comportamento Alimentar , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
16.
Zhonghua Xin Xue Guan Bing Za Zhi ; 33(1): 81-5, 2005 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-15924790

RESUMO

OBJECTIVE: Metabolic syndrome has attracted more attention from scientists of related areas due to its association with increased risk of cardiovascular disease and diabetes. The clinical identification criteria for metabolic syndrome issued by ATP III of NECP indicate the enlarged waist as the first component using the cut-offs derived from Caucasians. The purpose of this study is to investigate the appropriate cut-offs of waist circumference for Chinese adults as a component of metabolic syndrome. METHODS: Database of 13732 Chinese adults with 35 - 59 years from the risk factor survey in 1998 (the 9(th) Five Year National Project on trends and prediction of cardiovascular disease) was used to analyze the ORs of clustering of risk components by different strata of waist circumference. The sensitivity, specificity and distance in ROC curve by different cut-offs of waist circumference for identifying two or more risk components of metabolic syndrome were estimated to find the cut-off point for men and women with the shortest distance in ROC curve. RESULTS: The ORs of clustering of risk components increased significantly with the size of waist circumference. The waist circumference (>/= 85 cm for men, >/= 80 cm for women) corresponded to the shortest distance in ROC curve, namely, at these cut-offs, the rates of false positive and false negative for identifying clustering of two or more risk components were the minimum. CONCLUSIONS: If a person had three or more of the following components, metabolic syndrome could be defined: waist circumference >/= 85 cm in men or >/= 80 cm in women, SBP >/= 130 mm Hg or DBP >/= 85 mm Hg, TG >/= 1.69 mmol/L, HDL-C < 1.03 mmol/L and fasting blood glucose >/= 6.1 mmol/L. The prevalence of metabolic syndrome was 19.3% and 13.9% in middle-aged men and women respectively. In these patients the combination of enlarged waist, high blood pressure and high TG was the most frequent. This recommendation need further confirmed in representative sample of Chinese population.


Assuntos
Síndrome Metabólica/epidemiologia , Circunferência da Cintura , Adulto , Grupo com Ancestrais do Continente Asiático , Pressão Sanguínea , China/epidemiologia , HDL-Colesterol/sangue , Feminino , Humanos , Masculino , Síndrome Metabólica/sangue , Pessoa de Meia-Idade , Curva ROC , Sensibilidade e Especificidade
17.
Zhonghua Liu Xing Bing Xue Za Zhi ; 25(4): 308-11, 2004 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-15231197

RESUMO

OBJECTIVE: To study the relationship of timing of hospitalization and the severity, course, fatality of severe acute respiratory syndrome (SARS) patients. METHOD: 1291 hospital records of clinically diagnosed SARS patients with complete data gathered from "2003 Beijing SARS Clinical Database" were analyzed. RESULTS: SARS cases were categorized into four groups, according to the time of hospitalization after onset of the disease: within 3 days, during day 4 to day 7, during day 8 to day 14 and after day 14. The numbers of cases for each group were 568, 496, 177 and 50 respectively. Data showed that from group 1 to 4, the prevalence rates of major symptoms on the first day of hospitalization were: (1) 9.7%, 16.5%, 23.1% and 24.0% for "feeling chest pain" (P < 0.001), (2) 7.4%, 13.7%, 19.2% and 22.0% for "suffering from breathing obstruction" (P < 0.001), (3) 32.8%, 44.8%, 59.9% and 48.0%, for "coughing" (P < 0.001) and (4) 14.1%, 22.4%, 27.1% and 18.0% for "coughing up phlegm" (P = 0.0002), respectively. The rates of high respiratory frequency (>or= 24 bits/min.) were 11.1%, 15.5%, 22.8% and 25.5% (P < 0.001). The rates of abnormal chest X-ray were 80.3%, 89.0%, 92.3% and 88.9%, respectively (P = 0.002). The average numbers of abnormal lung field (the lung were divided into 6 fields) were 1.7, 1.9, 2.5 and 2.6 (P < 0.001); The numbers of cases receiving continuous oxygen supply treatment were 33.6%, 50.0%, 53.7% and 74.0% (P < 0.001), and the numbers of cases receiving glucocorticosteroids treatment were 28.2%, 35.9%, 53.7% and 62.0% (P < 0.001), respectively. With cases having had chronic baseline diseases prior to SARS infection, the age-standardized fatality rates were 14.9%, 11.7%, 50.0% and 33.9% (P < 0.001), and the average courses of the disease were 30.3, 34.2, 42.9 and 47.5 days (P < 0.001), respectively. In cases without chronic baseline diseases, the age-standardized fatality rates were 5.3%, 9.8%, 9.2% and 8.3% (P = 0.101), and the average courses for each group were 32.4, 35.3, 40.9 and 47.6 days (P < 0.001), respectively. CONCLUSION: Delayed hospitalization would cause the situation of SARS patient to deteriorate, losing the best chance for treatment and increase case fatality. In terms of control program on SARS, emphasize should be paid on decreasing the panic of patients to the disease so as to get early hospitalization.


Assuntos
Síndrome Respiratória Aguda Grave/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , China/epidemiologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Prognóstico , Síndrome Respiratória Aguda Grave/mortalidade , Síndrome Respiratória Aguda Grave/patologia , Índice de Gravidade de Doença , Taxa de Sobrevida , Fatores de Tempo
18.
Zhonghua Liu Xing Bing Xue Za Zhi ; 25(11): 954-7, 2004 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-15769326

RESUMO

OBJECTIVE: To assess the relationship between incidence of ischemic stroke and alcohol consumption. METHODS: The information on alcohol consumption and other cardiovascular risk factors such as SBP, DBP, TC was collected among 10 populations in northern and southern parts of China according to the international standardized methods. A total number of 30 560 men and women aged 35 - 59 were enrolled and followed up for an average of 15.2 years. Complete data on 12,352 men was analyzed. RESULTS: The risk on ischemic stroke incidence showed a graded association. Compared with nondrinkers, the relative risks of incidence of ischemic stroke for those who drank alcohol less then 15 gram per day, 15-30 gram per day, 30-60 gram per day, above 60 gram per day were 0.86 (95% confidence interval 0.57-1.27), 1.20 (0.87-1.65), 1.26 (0.86-1.84) and 1.96 (1.30-2.93), respectively. CONCLUSION: Our study revealed that in male Chinese, heavy alcohol intake significantly increased the risk while mild alcohol drinking did not increase the risk of ischemic stroke.


Assuntos
Consumo de Bebidas Alcoólicas/efeitos adversos , Infarto Cerebral/epidemiologia , Acidente Vascular Cerebral/epidemiologia , Adulto , Infarto Cerebral/etiologia , China/epidemiologia , Feminino , Humanos , Incidência , Masculino , Fatores de Risco , Acidente Vascular Cerebral/etiologia
19.
Stroke ; 34(9): 2091-6, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12907817

RESUMO

BACKGROUND AND PURPOSE: The goal of this article is to clarify the proportion of stroke subtypes in China, where stoke is the most common cause of death. METHODS: A total of 16,031 first-ever strokes in subjects >or=25 years of age were identified in 1991 to 2000 from 17 Chinese populations through a community-based cardiovascular disease surveillance program in the China Multicenter Collaborative Study of Cardiovascular Epidemiology. World Health Organization diagnosis criteria were used for classification of stroke subtypes. RESULTS: CT scan rate of stroke cases reached a satisfactorily high level only after 1996 in the study populations. In 8268 first-ever stroke events from 10 populations with CT scan rate >75% in 1996 to 2000, 1.8% were subarachnoid hemorrhage, 27.5% were intracerebral hemorrhage, 62.4% were cerebral infarction, and 8.3% were undetermined stroke. The proportion of intracerebral hemorrhage varied from 17.1% to 39.4% and that for cerebral infarction varied from 45.5% to 75.9% from population to population. The ratio of ischemic to hemorrhagic stroke ranged from 1.1 to 3.9 and averaged 2.0). The 28-day fatality rate was 33.3% for subarachnoid hemorrhage, 49.4% for intracerebral hemorrhage, 16.9% for cerebral infarction, and 64.6% for undetermined stroke. CONCLUSIONS: In our study, ischemic stroke was more frequent and its proportion was higher than hemorrhagic stroke in Chinese populations. Although hemorrhagic stroke was more frequent in Chinese than in Western populations, the variation in the proportion of stroke subtypes among Chinese populations could be as large as or larger than that between Chinese and Western populations.


Assuntos
Acidente Vascular Cerebral/classificação , Acidente Vascular Cerebral/epidemiologia , Adulto , Distribuição por Idade , Idade de Início , Idoso , Hemorragia Cerebral/diagnóstico , Hemorragia Cerebral/epidemiologia , Infarto Cerebral/diagnóstico , Infarto Cerebral/epidemiologia , China/epidemiologia , Feminino , Hospitalização/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Mortalidade , Vigilância da População , População Rural/estatística & dados numéricos , Distribuição por Sexo , Acidente Vascular Cerebral/diagnóstico , Hemorragia Subaracnóidea/diagnóstico , Hemorragia Subaracnóidea/epidemiologia , Tomografia Computadorizada por Raios X/estatística & dados numéricos , População Urbana/estatística & dados numéricos
20.
Zhonghua Nei Ke Za Zhi ; 42(2): 94-7, 2003 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-12783704

RESUMO

OBJECTIVE: To study the ratio of ischemic to hemorrhagic stroke incidence in Chinese populations. METHODS: Fifteen populations in China, each including about 100,000 residents, were monitored from 1991 to 2000 for the occurrence of stroke. Stroke was classified as hemorrhagic, ischemic and unclassified based on the clinical data and CT scan results respectively. RESULTS: The average CT scan rate in stroke patients from 1996 to 2000 varied from 14.8% to 97.5% among the 15 population and the average ratio of ischemic to hemorrhagic stroke ranged from 0.23:1 to 4.38:1; the correlation coefficient between the ratio and CT scan rate is 0.7 (P = 0.003). In all the populations with CT scan rate greater than 80%, the ischemic to haemorrhagic ratios were all greater than 1.0. From 1991 to 2000 in 12 populations with complete data, the CT scan rate in stroke patients rose from 41.1% to 88.3% and the ischemic to hemorrhagic ratio rose from 1.25:1 to 1.85:1. The correlation coefficient between the ratio and the CT scan rate was 0.9 (P < 0.001). In patients having CT scan, the ischemic to hemorrhagic ratio was greater than 1.0 in all the years. CONCLUSION: The predominant type of stroke in Chinese population is ischemic. The low CT scan rate in some populations is the main reason for the false impression predominantly hemorrhagic stroke.


Assuntos
Isquemia Encefálica/epidemiologia , Hemorragia Cerebral/epidemiologia , Acidente Vascular Cerebral/epidemiologia , Adulto , Idoso , Isquemia Encefálica/diagnóstico por imagem , Hemorragia Cerebral/diagnóstico por imagem , China/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Acidente Vascular Cerebral/diagnóstico por imagem , Tomografia Computadorizada por Raios X
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