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1.
Sci Total Environ ; 754: 142085, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32898782

RESUMO

BACKGROUND: Endocrine-disrupting chemicals (EDCs) are considered to be related to diabetes, but studies of the association between phenolic EDCs and gestational diabetes mellitus (GDM) are limited. OBJECTIVES: To assess associations of maternal urinary bisphenol A (BPA), nonylphenol (NP), and 2-tert-octylphenol (2-t-OP) with GDM occurrence. METHODS: A cross-sectional study was performed among 390 Chinese women at 24-28 weeks of gestation. GDM was diagnosed with a 2-h 75-g oral glucose tolerance test (OGTT). BPA, NP, and 2-t-OP concentrations were determined in urine samples. Linear and logistic regression tests evaluated associations of BPA, NP, and 2-t-OP with blood glucose levels and GDM prevalence. RESULTS: The 2-t-OP concentrations in GDM patients were significantly higher than in non-GDM women with median values of 2.23 µg/g Cr and 1.79 µg/g Cr, respectively. No significant difference was observed in BPA and NP. Urinary 2-t-OP was positively associated with blood glucose levels after adjustment for several confounding factors and urinary BPA and NP. Higher 2-t-OP levels were associated with higher odds of GDM (OR: 5.78; 95% CI: 2.04, 16.37), whereas higher NP levels were associated with lower odds (OR: 0.22; 95% CI: 0.05, 0.85) in the adjusted models. In addition, compared to the first quartile of 2-t-OP, the adjusted odds ratios (ORs) and 95% confidence intervals (95% CIs) for GDM in the second, third, and fourth quartiles were 2.81 (1.23, 6.42), 3.01 (1.30, 6.93), and 5.49 (2.24, 13.46), respectively. CONCLUSION: Our study indicates that, for the first time to our knowledge, exposure to 2-t-OP is associated with a higher risk of GDM. However, higher NP exposure is associated with lower GDM risk. Further studies are necessary to affirm the associations of 2-t-OP and NP with GDM, and to elucidate the causality of these findings.

2.
Forensic Sci Int Genet ; 50: 102405, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33152624

RESUMO

Human touch samples represent a significant portion of forensic DNA casework. Yet, the generally low abundance of genetic material combined with the predominantly extracellular nature of DNA in these samples makes DNA-based forensic analysis exceptionally challenging. Human proteins present in these same touch samples offer an abundant and environmentally-robust alternative. Proteogenomic methods, using protein sequence variants arising from nonsynonymous DNA mutations, have recently been applied to forensic analysis and may represent a viable option looking forward. However, DNA analysis remains the gold standard and any proteomics-based methods would need to consider how DNA could be co-extracted from samples without significant loss. Herein, we describe a simple workflow for the collection, enrichment and fractionation of DNA and protein in latent fingerprint samples. This approach ensures that DNA collected from a latent fingerprint can be analyzed by traditional DNA casework methods, while protein can be proteolytically digested and analyzed via standard liquid chromatography-tandem mass spectrometry-based proteomics methods from the same touch sample. Sample collection from non-porous surfaces (i.e., glass) is performed through the application of an anionic surfactant over the fingermark. The sample is then split into separate DNA and protein fractions following centrifugation to enrich the protein fraction by pelleting skin cells. The results indicate that this workflow permits analysis of DNA within the sample, yet highlights the challenge posed by the trace nature of DNA in touch samples and the potential for DNA to degrade over time. Protein deposited in touch samples does not appear to share this limitation, with robust protein quantities collected across multiple human donors. The quantity and quality of protein remains robust regardless of fingerprint age. The proteomic content of these samples is consistent across individual donors and fingerprint age, supporting the future application of genetically variable peptide (GVP) analysis of touch samples for forensic identification.

3.
Artigo em Inglês | MEDLINE | ID: mdl-33145927

RESUMO

AIM: Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor (GHSR), has been found to be involved in the regulation of blood pressure; however, its effects in preeclampsia (PE) and the potential underlying mechanism remain poorly understood. In this study, we aimed to investigate the correlation between ghrelin and PE and reveal the possible mechanism underlying any relationship. METHODS: The levels of ghrelin and VEGF in the plasma of 6 early-onset PE (EOPE), 6 late-onset PE (LOPE) and 12 healthy pregnant (HP) women were detected using enzyme-linked immunosorbent assay (ELISA). The recombinant plasmid, pCDH-ghrelin, was designed to overexpress ghrelin in human umbilical vein endothelial cells (HUVECs). We analyzed angiogenesis in vitro and investigated the mechanism using MTT assay, colony formation assay, transwell migration assay, Matrigel-induced tube formation assay and western blotting. RESULTS: Ghrelin was significantly decreased in EOPE patients (P < 0.05) but elevated in LOPE patients compared to HP groups (P > 0.05). There was a significant decrease in plasma level of VEGF in EOPE and LOPE patients compared to the controls (P < 0.05). The proliferation, migration and tube formation ability of HUVECs were enhanced after transfection with pCDH-ghrelin. Ghrelin increased VEGF by activating the Jagged1/Notch2 pathway. CONCLUSION: Our study uncovered that ghrelin has the potential to improve endothelial function by promoting angiogenesis through Jagged1/Notch2/VEGF pathway.

4.
Artigo em Inglês | MEDLINE | ID: mdl-33179352

RESUMO

In this study, porphyrinic zirconium (Zr) MOFs were investigated as heterogeneous photocatalysts for photoinduced electron transfer-reversible addition-fragmentation chain transfer (PET-RAFT) polymerization of various monomers under a broad range of wavelengths, producing polymers with high monomer conversions, narrow molecular weight distributions, low dispersity and good chain-end fidelity. Screening of various porphyrinic Zr-MOFs (Zn) demonstrated that MOF-525 (Zn) with the smallest size had the best photocatalytic activity in PET-RAFT polymerization, due to the enhanced dispersion and light penetration. Oxygen tolerance and temporal control were also demonstrated during MOF catalyzed PET-RAFT. Results suggested that the polymerization rates were significantly affected by changing the size and surface area of MOFs, and the heterogeneous MOF photocatalysts could be easily separated and recycled for up to five independent PET-RAFT polymerizations without an obvious decrease in efficiency. Finally, the MOF photocatalysts were utilized to create three-dimensional polymeric objects with high resolution via visible light mediated stereolithography in an open-air environment.

5.
Pharmacotherapy ; 2020 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-33070357

RESUMO

STUDY OBJECTIVES: Vancomycin is a primary antibiotic for the treatment of severe infections in children with malignant hematological disease. However, precise dosing of vancomycin is difficult in children because of high interindividual variability and limited data of pharmacokinetic profiles. The present study aims to develop a population pharmacokinetic (PPK) model for vancomycin in Chinese pediatric patients with hematological malignancies. DESIGN: This was a retrospective pharmacokinetic study. SETTING: The setting for this study was a tertiary-care children's hospital. PATIENTS: This study included 92 pediatric patients with hematological malignancies who received vancomycin and experienced therapeutic drug monitoring from February 2017 to December 2018. MEASUREMENTS AND MAIN RESULTS: A PPK model was generated with a nonlinear mixed effects model. In addition, required doses to achieve target therapeutic concentrations were simulated based on the final model. A one-compartment model with first-order elimination fit the concentration data best. Actual body weight (BW) and glomerular filtration rate (GFR) were the significant influential factors on the clearance (CL) of vancomycin. The final PPK model for CL was CL (L/h) = 4.18  × GFR / 145 0.741 × BW / 25 K , K =  BW - 0.856 / BW - 0.856 + 6 . 53 - 0.856 , and the volume of distribution was 22.3 L. The model proved to be robust and reliable. Reference dosing regimens were proposed based on the final model. CONCLUSIONS: A PPK model of vancomycin was established for Chinese pediatric patients with hematological malignancies using a nonlinear mixed effects model, which provided a reference for the clinical application of vancomycin.

6.
Environ Technol ; : 1-21, 2020 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-33086947

RESUMO

The use of ammonium iron (II) sulfate ((NH4)2Fe(SO4)2) to remediate soil contaminated with Cr (VI) was assessed. (NH4)2Fe(SO4)2 effectively remediated soil contaminated with Cr (VI) and, acted as a fertilizer by supplying nitrogen because it contains ammonium. The effects of the (NH4)2Fe(SO4)2 dose, water content, pH of the soil and the contact time were investigated. The amount of Cr (VI) leached from the most-polluted soil, determined using a leaching toxicity procedure using optimized conditions, was 347.64 mg kg-1 when the soil was untreated and 6.74 mg kg-1 when the soil was treated with (NH4)2Fe(SO4)2. Bio-utilizable Cr contributed 59.44% and 0.16% of the total Cr contents of the untreated and treated soil, respectively. The relatively stable Cr species contributed 24.92% and 98.38% of the total Cr contents of the untreated and treated soil, respectively. The results indicated that adding (NH4)2Fe(SO4)2 markedly decreased the risk of Cr being released from heavily contaminated soil by decreasing the availability of Cr in the soil. Overall, the results indicated that adding (NH4)2Fe(SO4)2 causes some Cr (VI) in contaminated soil to be reduced to Cr (III), and to form a precipitate, which decreases the risk of Cr being released. (NH4)2Fe(SO4)2 can be applied to soil contaminated with Cr (VI) on a large scale because it is cheap and simple to achieve.

7.
Eur J Dermatol ; 30(4): 455-456, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32969818
8.
Eur J Dermatol ; 30(4): 456-457, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32969819
9.
Sci Total Environ ; 748: 141567, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32814302

RESUMO

The negative impact of residual fluorinated antibiotics on the ecosystem and human health are of great concern. However, only a few studies have been conducted on the factors that influence the bioaccumulation of fluorinated antibiotics in aquatic organisms. To investigate the effects of fluorine substituent, environmental concentration of antibiotics, and temperature on the bioaccumulation of florfenicol (FLO), thiamphenicol (TAP), ofloxacin (OFX), and pipemidic acid (PPA), crucian carp (Carassius carassius) were exposed to different concentrations of antibiotics and different temperatures for 21 days. The liver exhibited the highest antibiotic concentrations, with 315.4 ± 13.6 ng g-1 wet weight (ww), followed by the bile (279.4 ± 12.4 ng mL-1), muscle (53.1 ± 4.3 ng g-1 ww), and gills (37.1 ± 2.6 ng g-1 ww). The FLO and OFX containing the fluorine substituent were much easier to accumulate in crucian carp compared with their isonomic TAP and PPA, respectively. The fluorine substituent increased the bioaccumulation of the targeted antibiotics in crucian carp. In addition, the lower levels of antibiotics presented higher bioaccumulation potential, but the temperature had little effect on the bioaccumulation. These findings in the present study can provide further insight into the environmental behaviors and ecological risks of fluorinated antibiotics in the aquatic environment.


Assuntos
Carpas , Poluentes Químicos da Água , Animais , Antibacterianos , Bioacumulação , Ecossistema , Flúor
10.
J Am Chem Soc ; 142(40): 17093-17104, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-32833442

RESUMO

Combinatorial biosynthesis with fungal polyketide synthases (PKSs) promises to produce unprecedented bioactive "unnatural" natural products (uNPs) for drug discovery. Genome mining of the dothideomycete Rhytidhysteron rufulum uncovered a collaborating highly reducing PKS (hrPKS)-nonreducing PKS (nrPKS) pair. These enzymes produce trace amounts of rare S-type benzenediol macrolactone congeners with a phenylacetate core in a heterologous host. However, subunit shuffling and domain swaps with voucher enzymes demonstrated that all PKS domains are highly productive. This contradiction led us to reveal novel programming layers exerted by the starter unit acyltransferase (SAT) and the thioesterase (TE) domains on the PKS system. First, macrocyclic vs linear product formation is dictated by the intrinsic biosynthetic program of the TE domain. Next, the chain length of the hrPKS product is strongly influenced in trans by the off-loading preferences of the nrPKS SAT domain. Last, TE domains are size-selective filters that facilitate or obstruct product formation from certain priming units. Thus, the intrinsic programs of the SAT and TE domains are both part of the extrinsic program of the hrPKS subunit and modulate the observable metaprogram of the whole PKS system. Reconstruction of SAT and TE phylogenies suggests that these domains travel different evolutionary trajectories, with the resulting divergence creating potential conflicts in the PKS metaprogram. Such conflicts often emerge in chimeric PKSs created by combinatorial biosynthesis, reducing biosynthetic efficiency or even incapacitating the system. Understanding the points of failure for such engineered biocatalysts is pivotal to advance the biosynthetic production of uNPs.

11.
Phys Chem Chem Phys ; 2020 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-32781462

RESUMO

Based on non-equilibrium Green's function combined with density functional theory (NEGF-DFT), we investigate the spin dependent transport in the ferromagnetic 6,6,12/γ-graphyne zigzag nanoribbon (GYZNR) heterojunction under different magnetic configurations. It is found that, at low bias ([-0.05, 0.1] V), the junction presents metallic transport with negligible spin polarization in parallel configuration (PC) while it behaves as an insulator in anti-parallel configuration (APC), which results in giant magnetoresistance. Interestingly, when we increase the bias voltage beyond [-0.05, 0.1] V, dual spin filtering characterized by electron transport of different spin channels under different polarity of bias is observed in APC but not in PC. All these findings are understood from the symmetry matching of wave functions in two nanoribbons at equilibrium or finite bias. Furthermore, dual spin filtering can also be achieved in PC by applying a gate voltage on the central interface region, which arises from the shift of different single spin channel of the central gate region into the bias window at a different polarity of the gate voltage. Thus, our work demonstrates the great potential of the 6,6,12/γ-GYZNR heterojunction as a multi-functional device and its great perspectives in carbon-based nanoelectronics and spintronics.

12.
BMC Infect Dis ; 20(1): 576, 2020 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-32758169

RESUMO

BACKGROUND: China ranks second in the world in terms of numbers of tuberculosis (TB) cases and is one of the top three countries with the largest number of multidrug-resistant and rifampicin-resistant TB (MDR/RR-TB). It also has high mortality and low cure rates of human immunodeficiency virus (HIV)-positive TB patients. This study aimed to analyse, under the integrated TB control model, the characteristics of TB patients seeking healthcare in the largest designated TB hospital in Chongqing. METHODS: This was a retrospective study of TB registers in a health facility. Record data of 1827 TB patients who had attended the Chongqing Public Health Medical Center (CPHMC) from 1 January to 31 December 2018 were included. The Statistical Package for Social Science (SPSS 18.0; IBM Corporation, Armonk, NY, USA) was used to analyse the data. Counting data were compared using the chi-square test or Fisher' s exact test. Among the results of the univariate analysis, the variables with statistical significance were included in the binomial stepwise logistic regression, with odds ratio and 95% confidence interval calculated. A two-tailed probability level of P < 0.05 was considered statistically significant. RESULTS: The majority of registered patients were men (1197), of Han ethnicity (1670), aged 21-60 years (1331), farmer/unemployed (1075), and living in county/district (1207). Approximately 24.9% of patients (455/1827) contracted DR-TB, 6% (110/1827) were co-infected with HIV, and 41.0% (749/1827) had drug-related hepatotoxicity. Among those patients, DR-TB was more likely to develop among farmers who received retreatment and had drug-related hepatotoxicity (P < 0.05). Women who received retreatment and lived in county/district were less likely to be HIV positive (P < 0.05). Compared with farmers, patients who were unemployed were more likely to be HIV positive, and those aged 21-60 years had a higher risk of being tested as HIV positive (P < 0.05). CONCLUSION: Farmers who received retreatment and had drug-related hepatotoxicity are more susceptible to DR-TB; young unemployed men have a higher risk of contracting HIV-positive TB. The demographic and clinical characteristics of TB patients should be taken into consideration in DR-TB and HIV-positive TB screening in the future.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Doença Hepática Induzida por Substâncias e Drogas/epidemiologia , Coinfecção/epidemiologia , HIV , Mycobacterium tuberculosis/efeitos dos fármacos , Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/virologia , Adulto , Antituberculosos/efeitos adversos , Antituberculosos/uso terapêutico , China/epidemiologia , Coinfecção/tratamento farmacológico , Coinfecção/virologia , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Fazendeiros , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico , Tuberculose Resistente a Múltiplos Medicamentos/microbiologia , Desemprego , Adulto Jovem
13.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 32(7): 840-845, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-32788020

RESUMO

OBJECTIVE: To evaluate the effectiveness and safety of early exercise safety management in patients undergoing mechanical ventilation in intensive care unit (ICU). METHODS: A prospective historical control observation was conducted. Forty-five patients with severe respiratory failure undergoing mechanical ventilation admitted to the ICU of Affiliated Hospital of Jining Medical University from April to June in 2019 were enrolled in the observation group and implemented early exercise safety management, including establishing multidisciplinary safety management team with ICU doctors, ICU nurses, respiratory therapists, rehabilitation therapists, dietitians and psychological consultants to jointly develop early exercise plan; equipping with sports and safety protection equipment; assessing the early exercise risks, formulating early exercise prescriptions; formulating the nutritional and psychological prescriptions; carrying out the propaganda and education in the early exercise process of patients and communicating with patients timely; strengthening team training. Other 45 patients with severe respiratory failure admitted for mechanical ventilation from January to March in 2019 were enrolled in the control group, whose gender, age and artificial airway conditions were matched with those in the observation group. Routine mechanical ventilation nursing was performed, including condition monitoring, medication nursing, environmental management and routine examination assistance. The improvement of depression, anxiety, comfort, lung function, and quality of life before and after nursing intervention were observed, and the occurrence of complications was recorded. RESULTS: There was no significant difference in gender, age or artificial airway conditions between the two groups. Before nursing intervention, there was no significant difference in depression, anxiety, comfort, lung function and quality of life between the two groups. After the intervention of different nursing programs, the above conditions of the two groups were significantly improved, and the improvement was more significant in the observation group, which showed that the hospital anxiety and depression scale (HAD) score and forced vital capacity (FVC) of the observation group were significantly lower than those of the control group [HAD score: 10.80±2.54 vs. 17.51±3.66, FVC (L): 1.81±0.42 vs. 2.23±0.39, both P < 0.01], while the general comfort questionnaire (GCQ) score, forced expiratory volume in 1 second (FEV1), FEV1/FVC ratio, FEV1 percentage of predicted (FEV1%) and each dimension score of 36-item short form health survey (SF-36) scale were significantly higher than those of the control group [GCQ score: 110.87±5.33 vs. 96.93±3.02, FEV1 (L): 1.99±0.37 vs. 1.71±0.15, FEV1/FVC ratio: 0.88±0.04 vs. 0.84±0.03, FEV1%: (88.98±8.57)% vs. (80.41±4.45)%, mental function score: 49.74±9.88 vs. 40.17±8.95, physical function score: 27.65±9.46 vs. 20.32±9.53, social relationship score: 62.14±6.33 vs. 50.28±8.76, general health score: 38.61±8.48 vs. 30.63±8.93, all P < 0.01]. The overall incidence of complications in the observation group was significantly lower than that in the control group (24.44% vs. 57.78%, P < 0.01). CONCLUSIONS: Early exercise safety management scheme for patients undergoing mechanical ventilation in ICU can improve clinical efficacy and reduce complications. At the same time, it can further standardize the behaviors of medical staff and ensure the early exercise.


Assuntos
Qualidade de Vida , Respiração Artificial , Humanos , Unidades de Terapia Intensiva , Estudos Prospectivos , Gestão da Segurança
14.
Orthod Craniofac Res ; 2020 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-32779352

RESUMO

The association between tooth movement and remodelling of surrounding bone is controversial. To analyse the effect of tooth movement on alveolar bone changes in maxillary and mandibular anterior teeth by cone-beam computed tomography (CBCT). The Embase, Cochrane Library and Medline databases were searched without any language restrictions. Longitudinal studies using CBCT to observe alveolar bone changes of maxillary and mandibular anterior teeth during orthodontic treatment were included. Two independent reviewers performed the study selection, data extraction and methodological quality assessment. A total of 26 studies were included in this review, 14 of which were eligible for quantitative synthesis. In extraction cases, meta-analysis showed vertical bone loss on the labial (0.36 mm) and lingual (0.94 mm) sides of maxillary incisors, and lingual bone thickness decreased significantly at the cervical level (0.57 mm). In non-extraction cases, vertical alveolar bone loss was significant on the labial side (0.97 mm) and lingual side (0.86 mm) of mandibular incisors. Subgroup analysis for skeletal class III patients indicated that vertical alveolar bone loss was 1.16 mm on the labial side and 0.83 mm on the lingual side of mandibular incisors. The absence of high-quality studies and the high heterogeneity of the included studies were limitations of this systematic review and meta-analysis. Based on limited evidence, alveolar bone height and thickness, especially at the cervical level, decreased during both labial and lingual movement of anterior teeth.

15.
Biometrics ; 2020 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-32799339

RESUMO

We propose a novel regularized mixture model for clustering matrix-valued data. The proposed method assumes a separable covariance structure for each cluster and imposes a sparsity structure (eg, low rankness, spatial sparsity) for the mean signal of each cluster. We formulate the problem as a finite mixture model of matrix-normal distributions with regularization terms, and then develop an expectation maximization type of algorithm for efficient computation. In theory, we show that the proposed estimators are strongly consistent for various choices of penalty functions. Simulation and two applications on brain signal studies confirm the excellent performance of the proposed method including a better prediction accuracy than the competitors and the scientific interpretability of the solution.

16.
Biomacromolecules ; 21(9): 3887-3897, 2020 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-32786533

RESUMO

In this study, we report dual roles for doxorubicin (DOX), which can serve as an antitumor drug as well as a cocatalyst for a photoliving radical polymerization. DOX enhances the polymerization rates of a broad range of monomers, including acrylamide, acrylate, and methacrylates, allowing for high monomer conversion and well-defined molecular weights under irradiation with a blue light-emitting diode light (λmax = 485 nm, 2.2 mW/cm2). Utilizing this property, the photopolymerization of N,N-diethylacrylamide was performed in the presence of a poly(oligo(ethylene glycol) methyl ether acrylate) macroreversible addition-fragmentation chain transfer (macroRAFT) agent to prepare polymeric nanoparticles via aqueous polymerization-induced self-assembly (PISA). By varying the monomer:macroRAFT ratio, spherical polymeric nanoparticles of various diameters could be produced. Most notably, DOX was successfully encapsulated into the hydrophobic core of nanoparticles during the PISA process. The DOX-loaded nanoparticles were effectively uptaken into tumor cells and significantly inhibited the proliferation of tumor cells, demonstrating that the DOX bioactivity was not affected by the polymerization reaction.

17.
Materials (Basel) ; 13(17)2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32825519

RESUMO

Coir fiber (CF), a renewable natural plant fiber, is more competitive in improving poor toughness and crack resistance of magnesium phosphate cement (MPC) than artificial fibers, due to its slight energy consumptions and low costs in production and waste treatment. In this paper, a typical three-point bending test was carried out to study the effects of CF length on MPC flexural properties. A total of forty-two cuboid specimens were employed to investigate the flexural strength, load-deflection behavior, and flexural toughness of MPC, with CF lengths varying from 0 to 30 mm at the curing age of 7 days and 28 days. Results showed that, at both two curing ages, MPC flexural strength first increased with CF length increasing, and then deceased when CF length exceeded the threshold. However, with the increase of CF length, MPC flexural toughness increased continuously, while MPC elastic modulus displayed a decreasing trend. Additionally, Modern micro testing techniques, such as scanning electron microscope (SEM) and X-ray diffraction (XRD), were also used to study the microstructure and phase compositions of specimens for further explaining the themicroscopic mechanism.

18.
Eur J Clin Pharmacol ; 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32803289

RESUMO

PURPOSE: To evaluate the role of diltiazem on tacrolimus sparing in pediatric primary nephrotic syndrome (PNS) and its relation to CYP3A4, CYP3A5, ABCB1, and SLCO1B3 polymorphisms. METHODS: The PNS children treated with tacrolimus and with steady-state trough concentration (C0) were retrospectively collected. The impacts of diltiazem on tacrolimus dose-adjusted C0 (C0/D), target concentration achievement, and required dose were evaluated. Meanwhile, the relationship between the polymorphisms (including CYP3A4*1G, CYP3A5*3, ABCB1-C3435T, and SCLO1B3) and dose-sparing effect were investigated. RESULTS: A total of 71 children with 535 concentrations, including 16 children with concomitant diltiazem, were involved. Significantly increased C0/D (94.0 vs 83.8 ng/mL per mg/kg, p = 0.038) and lower required daily dose of tacrolimus (0.056 vs 0.064 mg/kg, p = 0.003) were observed in patients co-administered with diltiazem. Subpopulation carrying CYP3A4*1G, CYP3A5*1, ABCB1-3435TT, or SLCO1B3-699AA was presented with enhanced increment in tacrolimus C0/D by 38.8-102.9%. CONCLUSION: Moderate effect of diltiazem on tacrolimus sparing, which might relate to the polymorphisms of CYP3A4, CYP3A5, ABCB1, and SLCO1B3, was documented.

19.
Langmuir ; 36(32): 9443-9448, 2020 08 18.
Artigo em Inglês | MEDLINE | ID: mdl-32693594

RESUMO

The popularity of humidity sensing for respiratory analysis of patients is gradually increasing because of its portability and cost-effectiveness. However, current flexible humidity sensors are mainly made of polymer films, whose poor hygroscopicity and breathability reduce their sensitivity and comfort. In this study, a highly sensitive humidity sensor was developed using non-woven fabric (NWF) coated with graphene oxide (GO). Bovine serum albumin was used to improve the adsorption of GO onto the NWF, and its effect on sensitivity was investigated by adjusting its concentration. High-humidity sensitivity was experimentally validated by testing different relative humidity levels, and its fast response and excellent feasibility under diverse breathing conditions were verified by successful monitoring of fast and deep breathing, differentiating nose and mouth breathing, and even identifying simple spoken words. This study developed a breathable and skin-friendly humidity sensor based on GO/NWF, which is a promising device for human healthcare.

20.
Materials (Basel) ; 13(14)2020 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-32708951

RESUMO

Coir fiber (CF), an eco-friendly and renewable natural fiber, was introduced into magnesium phosphate cement (MPC) mortar to improve its crack resistance. A total of 21 specimens were employed to investigate the failure pattern, compressive strength, stress-strain curve, and energy absorption of MPC with varying CF lengths (0, 5, 10, 15, 20, 25, and 30 mm) after a curing period of 28 days through a static compressive test. The results demonstrated that compressive strength, elastic modulus, and secant modulus decreased with the increase in CF length. However, energy absorption presented a convex curve, which increased to the maximum value (77.0% relative to the value of the specimen without CF) with a CF length of 20 mm and then declined. A series of modern micro-tests were then carried out to analyze the microstructure and composition of specimens to explain the properties microscopically.

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