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1.
Biomed Pharmacother ; 121: 109552, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31715370

RESUMO

Rhodiola rosea L., a worldwide botanical adaptogen, has been confirmed to possess protective effects of inflammatory injury for many diseases, including cardiovascular diseases, neurodegenerative diseases, diabetes, sepsis, and cancer. This paper is to review the recent clinical and experimental researches about the anti-inflammatory effects and the related mechanisms of Rhodiola rosea L. extracts, preparations, and the active compounds. From the collected information reviewed, this paper will provide the theoretical basis for its clinical application, and provide the evidences or guidance for future studies and medicinal exploitations of Rhodiola rosea L.

2.
Waste Manag ; 101: 180-187, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31622863

RESUMO

With the rapid development of photovoltaic industry, the recycling of waste solar photovoltaic (PV) panels is becoming a critical and global challenge. Considering PV panels recycling is significantly effective and worthwhile to save natural resources and reduce the cost of production, how to selectively recycle valuable components of PV panels is the hot and dominant topic. Different from current mechanical crushing, heat treatment and chemical operation processes, novel and environment-friendly recycling approaches by using high voltage pulse discharge in water, called high voltage fragmentation (HVF), was discussed under different discharge conditions. The results showed that discharging across surface and interior of PV panels produced ablation round holes, sputter metal particles and dendritic channels. The average particle size decreased with the ascent of pulse number and voltage amplitude. Considering the energy consumption, the optimal condition of HVF in this paper was 160 kV for 300 pulses with the energy consumption of 192.99 J/g, crushing the PV panels into particles of 4.1 mm in average (13.7% of the initial size). More particle was distributed among the 0.1-2 mm size fractions as the energy increased. Selective fragmented products, such as Cu, Al, Pb, Ag and Sn, are concentrated on the fractions under 1 mm. Finally, hybrid crushing energy consumption model combined with fractal theory was discussed, which presented close relationship between energy and average particle size. Walker's model (n = 2.047 determined by fractal theory) had the best fitting effect.


Assuntos
Resíduo Eletrônico , Metais , Reciclagem
3.
Protein Expr Purif ; 166: 105521, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31654735

RESUMO

Nuclear magnetic resonance (NMR) spectroscopy is a primary structural biology method to characterize protein dynamics in solution. For large macromolecular systems, methyl-labeling in a perdeuterated background significantly improves the relaxation properties, while providing sensitive probes for structure and dynamics analysis. However, how to prepare methyl-labeled proteins, especially for functional eukaryotic proteins, remains to be a major bottleneck in this field. Due to its advantages in eukaryotic co-translational and post-translational modification, as well as high-density fermentation, Pichia pastoris has been a cost-effective platform strain for 13C, 15N-labeling and deuterium labeling since the early 2000's. Recently, some substantial progress has been made in methyl-labeling, such as the feasibility of 13C isoleucine δ1 methyl-labeling in perdeuterated background and the increased uptake of the Val/Leu precursor. Here, we systematically introduce the isotope-labeling strategies in P. pastoris, including strain engineering and detailed fermentation protocols in 13C, 15N-labeling and methyl-labeling, providing instructions and guidance for the future improvement of sample preparation for NMR spectroscopy.

4.
J Hazard Mater ; 383: 121169, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31520931

RESUMO

Nickel (Ni) contamination in soils, at high concentrations, is considered to be very common. Knowledge of the total content of Ni is frequently insufficient to estimate environmental risk. Our explored findings showed that the earthworms adding reduced the available Ni, along with the superior performance of HCl than CaCl2. The bioaccumulation of Ni in earthworms was aggravated with increasing Ni dosage and exposure time. Bioaccumulation factor was significantly correlated with the extractable Ni, which was the most suitable predicting the variations of Ni bioavailability. LC50 of earthworms on 7 and 14 days were 1202.444 mg kg-1 and 1069.324 mg kg-1, respectively along with the recovery rate in 500 mg kg-1 Ni polluted soil reached up to 92.5%. Earthworms' respiration was sensitive presenting a significant dose-effect relationship with the Ni concentration. Five biochemical indices in earthworms were induced along with the relevance of a dose- and time-response pattern. Additionally, histological damage in earthworm's body wall, intestine and seminal vesicles were observed under high level of Ni exposure. Overall, we believe that our current study will open a new window for deeper insights into the potential availability of Ni along with other associated metals on the function of soil ecosystem.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31525458

RESUMO

Cladocera are small freshwater crustaceans that have attracted considerable attention in recent years. They are commonly used for studying senescence. In this study, we used LC-MS/MS with eight-plex iTRAQ to perform a comparative proteomic analysis of senescence in Daphnia pulex. Of 3076 primordial proteins, 2325 were credible (the remaining were low-confidence proteins) and 247 significantly differentially expressed proteins (DEPs). Of the latter, 87, 91, and 69 DEPs were identified in the Day 15 vs. Day 5, Day 20 vs. Day 5, and Day 25 vs. Day 5 groups, respectively. Gene ontology enrichment analysis showed that oxidative damage may be the main cause of senescence in D. pulex. Using Kyoto Encyclopedia of Genes and Genomes pathway analysis, we found that the peroxisome pathway played an important role in aging. Our results suggest that D. pulex alleviates excessive oxidative damage by altering key enzymes involved in carbohydrate and protein metabolism.

6.
J Exp Child Psychol ; 189: 104703, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31655228

RESUMO

From an early age, children are able to use surface layout geometry and landmarks to search for a hidden toy when disoriented. Theoretical debate remains regarding whether children represent locations based on the global environment or on local cues. Exploring whether children construct and use the relationships between discrete locations of the global environment can provide direct evidence regarding this issue. We investigated young children's representation of two geometric relationships: diagonal relationships (Experiment 1) and same-side relationships (Experiment 2). Children (4- and 5-year-olds) were tested in a square room with a distinctively colored wall. Children completed two tasks. In a two-location task, children watched two toys hidden in two corners that formed one of the two relationships. After disorientating children, the experimenter uncovered one toy and children searched for the other one (target). In a one-location task, only one toy was hidden. In both experiments, children's performance was better in the two-location task than in the one-location task. Furthermore, accuracy in the two-location task of Experiment 1, in which the two corners formed a diagonal relationship, was higher than that of Experiment 2, in which the two corners formed a same-side relationship and a correct location required the combination of this relationship and landmark. These findings suggest that at least by 4 years of age, children can construct geometric relationships between individual corners in their spatial representation and support the global accounts of young children's location coding.

7.
J Nanosci Nanotechnol ; 20(4): 2442-2451, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492260

RESUMO

Two methods of TiO2 addition were applied to prepare hydroxyapatite/TiO2 (HA/TiO2) composite, i.e., in-situ hydrolysis TiO2 in HA powders (N-HA/TiO2) and mixing commercial nano-sized HA and TiO2 powder (C-HA/TiO2). Effects of TiO2 addition methods and sintering temperatures on phase, microstructure and microhardness were investigated for pressureless sintered HA/TiO2 composites, and pure HA was investigated for comparison. Results show that TiO2 from both in-situ hydrolysis and mixing commercial powder presented similar effects on phase structures and composition, and trended to chemically react with HA in the HA/TiO2 composites at high sintering temperature. Weight loss for different composites was investigated by thermal analysis. Sintering behavior for two different composite was also discussed. The TiO2 from in-situ hydrolysis can effectively enhance the TiO2 distribution and densification for the N-HA/TiO2 composites. Both two different composites showed typical grain growth and pore formation with the increase of sintering temperature. The N-HA/TiO2 composite had a lower porosity, higher shrinkage and microhardness than that of C-HA/TiO2 composite at sintering temperature from 700 °C to 1100 °C.

8.
Clin Chim Acta ; 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31794768

RESUMO

BACKGROUND AND AIMS: The role of monocyte lymphocyte ratio (MLR) in predicting the risk of chronic kidney disease (CKD) is unclear, although inflammation contributes to the development of CKD. This study aimed to investigate whether elevated MLR predicts new-onset CKD. METHODS: This study enrolled 14033 consecutively Chinese participants. The primary outcome was the new-onset CKD defined as an estimated glomerular filtration rate < 60 mL/min/1.73 m2 or the presence of proteinuria after follow-up. After the descriptive analyses of baseline data, Univariate and multivariate Cox proportional models were used to evaluate the independent relationship between MLR and new-onset CKD. RESULTS: 11280 participants were included in the final analysis, and 58.44%(n=6592) of them were male. The mean age was 44.67 ± 12.85 years. After a median follow-up of 1.94 years, 2.55% (n=288) of participants developed new-onset CKD. MLR was associated with the increased risk of CKD (HR=16.12, 95% CI=4.52-57.56, p<0.0001). After adjustment for age, gender, body mass index, history of hypertension, systolic blood pressure, high-density lipoprotein cholesterol, triglyceride, fasting plasma glucose, uric acid and estimated glomerular filtration rate, MLR remained an independent risk factor for CKD (HR=8.89, 95%CI=2.18-36.27, p=0.0023). CONCLUSION: MLR is an independent predictor of the risk of CKD, which might be expected to better guide early prevention and treatment interventions.

9.
Sci Total Environ ; : 135269, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31796282

RESUMO

Low underwater light availability and benthivorous fish-mediated disturbance are two important factors that influence the growth of submersed macrophytes. However, the combined effects of these factors remain unclear. To determine the combined effects of low light and fish-mediated disturbance on the growth of two submersed macrophytes with contrasting growth forms, i.e., Vallisneria natans and Hydrilla verticillata, we conducted an outdoor mesocosm experiment with a two-by-two factorial design. The experiment involved two fish-mediated disturbance levels (0 and 1 Misgurnus anguillicaudatus) crossed with two levels of light intensity (ambient light and a low-light environment created by culturing the macrophytes under a shelter). The results showed that the chlorophyll a (chl a) concentration in the overlying water showed no difference among treatments for each macrophyte species. The fish-mediated disturbance significantly decreased the relative growth rate (RGR) of both species in the low-light environment but showed no effects in the ambient light environment. Low light availability and/or fish-mediated disturbance led to increased plant heights of both species compared with the heights under the ambient light regime. Low light availability combined with fish-mediated disturbance significantly reduced the ramet number and soluble carbohydrate (SC) content of both species; however, the free amino acid (FAA) content was not affected. Compared to V. natans, H. verticillata exhibited a high RGR and high ramet numbers in a low-light environment combined with fish-mediated disturbance. Our results indicated that the adaptability of H. verticillata is better than that of V. natans in turbid, shallow and hydrostatic water. Fish-mediated disturbance can negatively influence submersed macrophyte recovery in lakes when light is not abundant.

10.
Environ Int ; 135: 105347, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31794940

RESUMO

This study investigated perfluoroalkyl acids (PFAAs) in edible parts of vegetables, soils, and irrigation water in greenhouse and open filed, for the first time, in Shouguang city, the largest vegetable production base in China, which is located nearby a fluorochemical industrial park (FIP). The bioaccumulation factors (BAFs) were calculated, and the human exposures of PFAAs via consumption of the vegetables for different age groups assuming the maximum levels detected in each vegetable and average consumption rates were also estimated. The ΣPFAA levels ranged from 1.67 to 33.5 ng/g dry weight (dw) in the edible parts of all the vegetables, with perfluorobutanoic acid (PFBA) being the dominant compound with an average contribution of 49% to the ΣPFAA level. The leafy vegetables showed higher ΣPFAA levels (average 8.76 ng/g dw) than the fruit and root vegetables. For all the vegetables, the log10 BAF values of perfluorinated carboxylic acids showed a decreasing trend with increasing chain length, with PFBA having the highest log10 BAF values (average 0.98). Cabbage had higher bioaccumulation of PFBA (log10 BAF 1.24) than other vegetables. For the greenhouse soils and vegetables, the average contribution of perfluorooctanoic acid (PFOA) to ΣPFAA was lower than that in the open field samples, while the contributions of PFBA, PFHxA, PFPeA to ΣPFAA were higher. Irrigation water may be an important source of PFAAs in greenhouse, while for open field vegetables and soils, atmospheric deposition may be an additional contamination pathway. The estimated maximum exposure to PFOA through vegetable consumption for urban preschool children (aged 2-5 years) was 63% of the reference dose set by the European Food Safety Authority. Suggestions are also provided for mitigating the health risks of human exposure to PFAAs.

11.
Eur J Clin Pharmacol ; 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31786617

RESUMO

BACKGROUND: Accumulating evidence suggested that the use of metformin had more benefits for both prevention and treatment of tuberculosis (TB) than non-metformin use in patients with diabetes mellitus (DM); however, it remains to be fully elucidated on this topic. Thus, we conducted a systematic review and meta-analysis of published studies to determine the association between metformin use and TB in patients with diabetes. METHODS: The MEDLINE, EMBASE, Information Sciences Institute (ISI) Web of Science, and Cochrane CENTRAL databases were searched from their inception to 15 April 2019. Studies that evaluated the use of metformin and TB disease were included. The quality of each study was evaluated through the Newcastle-Ottawa Scale (NOS). For pooled data, the relative risk (RR) and 95% confidence intervals (CIs) were calculated; otherwise, a systematic review. RESULTS: Seventeen observational studies were included, all of which indicated a low risk of bias according to the NOS. The pooled analysis showed that metformin use was associated with a significantly lower active TB incidence and mortality among individuals with DM (RR = 0.51; 95% CI, 0.38-0.69, P ⩽ 0.001) and with TB-DM (RR = 0.34; 95% CI, 0.20-0.57, P ⩽ 0.001), respectively. CONCLUSIONS: This meta-analysis indicated metformin use is related to benefits in both prevention and treatment outcomes of tuberculosis among patients with diabetes. Prospective clinical trials are needed to confirm these associations.

12.
BMC Nephrol ; 20(1): 439, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31791277

RESUMO

BACKGROUND: In recent years, there has been a growing concern that abdominal aortic calcification (AAC) has a predictive effect on the prognosis of patients with end-stage renal disease (ESRD). However, whether other vascular calcification (VC) can predict the occurrence of adverse events in patients, and whether it is necessary to assess the calcification of other blood vessels remains controversial. This study aimed to assess VC in different sites using X-ray films, and to investigate the predictive effects of VC at different sites on all-cause mortality and cardiovascular (CV) mortality in peritoneal dialysis (PD) patients. METHODS: The data of Radiographs (lateral abdominal plain film, frontal pelvic radiograph and both hands radiograph) were collected to evaluate the calcification of abdominal aorta, iliac artery, femoral artery, radial artery, and finger arteries. Patients' demographic data, clinical characteristics, laboratory data were recorded. The total follow-up period was 8 years, and the time and cause of death were recorded. Survival curves were estimated using Kaplan-Meier analysis. COX regression analysis was used to examine independent predictors of all-cause mortality and CV mortality. RESULTS: One hundred fifty PD patients were included, a total of 79 patients (52.7%) died at the end of follow-up. After adjusting variables in the multivariate COX regression analysis, AAC was an independent predictor of all-cause mortality in PD patients (HR = 2.089, 95% CI: 1.089-4.042, P = 0.029), and was also an independent predictor of CV mortality (HR = 4.660, 95% CI: 1.852-11.725, P = 0.001). We also found that femoral artery calcification had a predictive effect on all-cause and CV mortality. But the calcification in iliac artery, radial artery, and finger arteries were not independent predictors of patients' all-cause and CV mortality in PD patients. CONCLUSION: AAC was more common in PD patients and was an independent predictor of all-cause mortality and CV mortality. The femoral artery calcification also can predict the mortality, but the calcification of iliac artery, radial artery, and finger arteries cannot predict the mortality of PD patients.

13.
Adv Mater ; : e1904011, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31793717

RESUMO

Biodegradable nanoprodrugs, inheriting the antitumor effects of chemotherapy drugs and overcoming the inevitable drawback of side effects on normal tissues, hold promise as next-generation cancer therapy candidates. Biodegradable nanoprodrugs of transferrin-modified MgO2 nanosheets are developed to selectively deliver reactive oxygen species to cancer cells for molecular dynamic therapy strategy. The nanosheets favor the acidic and low catalase activity tumor microenvironment to react with proton and release nontoxic Mg2+ . This reaction simultaneously produces abundant H2 O2 to induce cell death and damage the structure of transferrin to release Fe3+ , which will react with H2 O2 to produce highly toxic ·OH to kill tumor cells.

14.
Stem Cell Res ; 41: 101607, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31778937

RESUMO

Familial Parkinson's disease (PD) can be caused by deleterious mutations in PINK1 (encoding PINK1) in an autosomal recessive manner. Functional studies suggest that PINK1 works as a regulator of mitochondrial homeostasis. However, how loss of PINK1 induces dopaminergic neuron degeneration is still unclear. Here, we have generated a patient-derived induced pluripotent stem cell (iPSC) line with mutant PINK1 (p. I368N). This cell line will facilitate PD disease modeling in vitro and can be used for generating isogenic cell lines through gene correction.

15.
Chemosphere ; 242: 125078, 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31704520

RESUMO

The widespread use of tetrabromobisphenol A (TBBPA) in industries has resulted in its frequent detection in environmental matrices, and the mechanisms of its associated hazards need further investigation. In this study, the nematode Caenorhabditis elegans (C. elegans) was exposed to environmentally relevant concentrations of TBBPA (0, 0.1, 1, 10, 100, 200 µg/L) to determine its effects. At TBBPA concentrations above 1 µg/L, the number of head thrashes, as the most sensitive physiological indicator, decreased significantly. Using the Illumina HiSeq™ 2000 sequencer, differentially expressed genes (DEGs) were determined, and 52 were down regulated and 105 were up regulated in the 200 µg/L TBBPA treatment group versus the control group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database analysis demonstrated that dorso-ventral axis formation is related to neurotoxicity; metabolism of xenobiotics by Cytochrome P450 (CYP450) and glutathione-S-transferase (GST) was found to be the vital metabolic mechanisms and were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). GST was ascribed to the augmentation because mutations in cyp-13A7 were constrained under TBBPA exposure. Additionally, oxidative stress indicators accumulated in a dose-dependent relationship. These results will help understand the molecular basis for TBBPA-induced toxicity in C. elegans and open novel avenues for facilitating the exploration of more efficient strategies against TBBPA toxicity.

16.
Org Lett ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31710499

RESUMO

The efficient synthetic route was disclosed to prepare optically active triarylborane-based [5]helicenes, 7B-PhHC and 7B5N-PhHC. Their emission wavelengths are tunable by both the chemical structure modification and the tuning of excited state charge transfer dynamics via selection of appropriate solvents or addition of external F-, enabling the full-color circularly polarized luminescence with moderate to good quantum yields (0.07-0.51) and high luminescence dissymmetry factors (glum > 5 × 10-3).

17.
Artigo em Inglês | MEDLINE | ID: mdl-31720905

RESUMO

PURPOSE: To establish a single-nucleotide polymorphism-based analysis (SBA) method to identify triploidy in the miscarriage tissue by using low-coverage whole-genome sequencing (LC-WGS). METHODS: The method was established by fitting a quadratic curve model by counting the distribution of three heterozygous mutation content intervals. The triploid test result was mainly determined by the opening direction and the axis of symmetry of the quadratic curve, and Z test between the same batch samples was also used for auxiliary judgment. RESULTS: Two hundred thirteen diploid samples and 8 triploid samples were used for establishment of the analytical method and 203 unknown samples were used for blind testing. In the blind testing, we found 2 cases positive for triploidy. After chromosome microarray analysis (CMA) and mass spectrometry verification, we found that both samples were true positives. We randomly selected 5 samples from the negative samples for mass spectrometry verification, and the results showed that these samples were all true negatives. CONCLUSIONS: Our method achieved accurate detection of triploidy in the miscarriage tissue and has the potential to detect more chromosomal abnormality types such as uniparental disomy (UPD) using a single LC-WGS approach.

18.
Int Urol Nephrol ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31720952

RESUMO

OBJECTIVE: Levels of urinary prostatic exosomal protein (PSEP) were detected to evaluate the clinical potential of PSEP as a diagnostic marker of chronic prostatitis (CP). MATERIALS AND METHODS: The level of urinary PSEP was measured in 412 cases by an enzyme-linked immunosorbent assay kit, including 202 controls and 210 CP cases. Of the CP patients, 116 cases met the definition of the USA National Institutes of Health category III (NIH-III), with 60 cases of NIH-IIIA and 56 cases of NIH-IIIB. The ages, body mass indexes (BMI), white blood cell (WBC) levels in expressed prostatic secretions (EPS), lecithin body counts in EPS, urine PSEP levels both before and after prostate massage obtained from the CP patients and NIH-CPSI scores were analyzed. RESULTS: In the diagnosis of CP, the PSEP contents in the urine samples before and after prostate massage manifested a sensitivity of 86.93% vs. 61.06%, and a total coincidence rate of 85.24% vs. 61.06%, respectively. The area under the ROC curve was 0.926 vs. 0.709 for the before and after massage PSEP contents, respectively. Besides, during the follow-up of patients with CP, the improvement in symptoms was not correlated with the level changes of PSEP. CONCLUSION: Measurement of PSEP levels for the clinical diagnosis of CP is objective and painless. It could be a novel, simple, and noninvasive method for the diagnosis of CP. However, differences in fluid intake may result in a concentration or dilution of urine, which would ultimately affect the judgment of PSEP results.

19.
Neurosci Lett ; : 134596, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31711976

RESUMO

The occipital lobe has been implicated in anxiety disorder, however, its contributions to anxiety in healthy adults remain less clear. We conducted a resting-state functional magnetic resonance imaging study to explore the relationship between the amplitude of low-frequency fluctuation (ALFF), functional connectivity (FC), and state anxiety level in the healthy population. First, the results showed that the ALFF of the left inferior occipital gyrus (IOG) was negatively correlated with state anxiety. Furthermore, state anxiety was positively correlated with the FC between the left IOG and the right medial superior frontal gyrus and right cerebellum 8 area and negatively correlated with the FC between the left IOG and the left superior parietal gyrus. These results indicate that the occipital lobe of healthy individuals is involved in processing of anxiety in part through a frontal-parietal network.

20.
Sci Total Environ ; 694: 133803, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31756841

RESUMO

Bamboo forests are an important part of the forest ecosystem, which has strong carbon sequestration potential and plays an important role in the global carbon cycle. As a key parameter for simulating the carbon cycle using forest ecosystem models, the quality of leaf area index (LAI) data has a direct influence on the accuracy of modelling results. Here, we used the particle filter (PF) algorithm and PROSAIL model to assimilate MODIS LAI products, which were then used to drive a boreal ecosystem productivity simulator model to simulate the bamboo forest carbon cycle. The results showed that the relationship between the assimilated and observed LAI values was very significant, with an R2 of 0.95 and an RMSE of 0.28, greatly improving the precision of MODIS LAI products. The R2 values for the gross primary productivity (GPP), net ecosystem exchange (NEE), and total ecosystem respiration (TER) simulated by the assimilated LAI values and observed carbon fluxes were 0.65, 0.45 and 0.70, respectively, and the RMSE values were 1.10 g C m-2 day-1, 1.00 g C m-2 day-1 and 0.35 g C m-2 day-1, respectively. Compared with the results of the carbon cycle simulated by non-assimilated LAI, the R2 values of the GPP, NEE and TER values that were simulated by assimilated LAI increased by 27.5%, 45.2% and 6.1%, and the RMSE values decreased by 29.9%, 23.7% and 22.2%, respectively. Therefore, coupling the PF and PROSAIL models can greatly improve the simulation precision for the large-scale bamboo forest carbon cycle. This study laid the foundation for simulating the carbon cycle over a large-scale bamboo forest based on low-resolution data in the future.

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