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1.
J Adv Nurs ; 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34643959

RESUMO

AIMS: To explore the role of self-efficacy (SE) in the effect of patient empowerment on self-management behaviours among patients with chronic illness and to investigate the moderating effect of three types of health locus of control (HLC) in this moderated mediation model. DESIGN: Cross-sectional design. METHODS: Data were collected in a general tertiary hospital, and a sample of 254 patients was recruited between August and October 2020. The effect of moderation and mediation was tested by the PROCESS macro (Model 4 and Model 8) for SPSS 25.0 by Hayes using 5000 bootstrap samples. RESULTS: Self-efficacy significantly mediated the relationship between patient empowerment and self-management behaviour with a 95% confidence interval excluding zero. The chance HLC demonstrated a moderating effect, and the interaction effect on SE and self-management behaviour was significant. CONCLUSION: Patient empowerment may improve confidence and adherence to self-management among people with chronic illness, and such benefits were conditional on the HLC of patients. IMPACT: This study addresses the relationship between patient empowerment and self-management behaviour in patients with different personality characteristics. This result indicated that classifying the type of HLC may enable the identification of subgroups of patients who may subsequently benefit from patient empowerment. In a patient-centred programme, nurses and other healthcare professionals correctly identifying patients' HLC type and understanding the implications and then providing appropriate health care plans for patients with different health beliefs may be useful to tailor the decision-making process.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34610781

RESUMO

AIM AND OBJECTIVES: The Gochnatia decora (Kurz) A. L. Cabrera is a rare woody plant belonging to the family of Asteraceae. The bark of this plant is used in Chinese folk medicine to treat cough and pneumonia. However, the effective substance related to its efficacy remains unknown. This study aims to evaluate the potential anti-inflammatory activities of the chemicals isolated from this plant using a model of LPS-induced RAW264.7 cells. MATERIAL AND METHODS: Chemical constituents were isolated from the stems and leaves of G. decora by a series of chromatographic separation methods and identified by spectral analysis techniques. The model of inflammation in vitro was established by treatment of 1 µM LPS on RAW264.7 cells. The influence of tested compounds on inflammatory factor production, including NO, TNF-α, IL-1ß, IL-6, IL-17, were determined by ELISA. The mechanisms involved were studied by western blot analysis. RESULTS: Two known ent-kaurane diterpenes (1 and 2), identified as ent-17-hydroxy-15-oxokauran-19-oic acid (1) and ent-15α-hydroxy-16-kauran-19-oic acid (2), were isolated from the stems and leaves of G. decora. The bioassay showed both of them produced significant inhibition of LPS-induced release of NO, TNF-α, IL-1ß, IL-6, IL-17, iNOS, and COX-2 expression. Western blot analysis showed that these same 2 chemicals blocked LPS-induced phosphorylation of NF-κB. CONCLUSIONS: Compounds 1 and 2 were obtained from the genus Gochnatia. These compounds demonstrated useful anti-inflammatory activities in the model of LPS-induced RAW264.7 cells. A potential action mechanism may be correlation of the NF-κB pathway.

3.
Nat Prod Res ; : 1-6, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34583592

RESUMO

Two new sesquiterpenes (1-2) and six known analogues (3-8) were isolated from the branches and leaves of Xylopia vielana Pierre. The structures of the new compounds were identified by analyzing 1 D and 2 D NMR data and HRESIMS data, combined with induced and calculated circular dichroism experiments. In addition, compounds 1-4, 7 and 8 showed notable nitric oxide (NO) inhibitory effects (IC50 < 10 µM) on the model of the lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.

5.
Adv Mater ; : e2105002, 2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34561905

RESUMO

The precise tuning and multi-dimensional processing of covalent organic frameworks (COFs)-based materials into multicomponent superstructures with appropriate diversity are essential to maximize their advantages in catalytic reactions. However, up to now, it remains an ongoing challenge for the precise design of COFs-based multicomponent nanocomposites with diverse architectures. Herein, a metal organic framework (MOF)-sacrificed in situ acid-etching (MSISAE) strategy that enables continuous synthesis of core-shell, yolk-shell, and hollow-sphere COFs-based nanocomposites through tuning of core decomposition (NH2 -MIL-125 into TiO2 ) rate is developed. More importantly, due to the multiple active sites, fast transfer of carriers, increased light utilization ability, et al, one of the obtained samples, NH2 -MIL-125/TiO2 @COF-366-Ni-OH-HAc (yolk-shell) with special three components, exhibits high photocatalytic CO2 -to-CO conversion efficiency in the gas-solid mode. The MSISAE strategy developed in this work achieves the precise morphology design and control of multicomponent hybrid composites based on COFs, which may pave a new way in devealoping porous crystalline materials with powerful superstructures for multifunctional catalytic reactions.

6.
Artigo em Inglês | MEDLINE | ID: mdl-34341834

RESUMO

BACKGROUND: Increasing evidence has shown that the occurrence and development of various human diseases are closely related to the gut microbiota. We compared the gut microbial communities of human subjects with congenital insensitivity to pain with anhidrosis (CIPA) and healthy controls (HCs) to assess whether fecal microbiota transplantation (FMT) into germ-free mice and mice in acute pain influenced the behaviors of the host. METHODS: We utilized 16 s rRNA analysis to compare the gut microbial communities of CIPA subjects and HCs and assessed whether FMT into germ-free mice and mice in acute pain influenced the behaviors of the host. RESULTS: In a 16 s RNA analysis, the CIPA group had significant decreases in the relative abundance of 11 bacteria, whereas 7 bacteria were significantly increased. In further animal experiments, the transplantation of fecal samples from CIPA patients to healthy mice significantly increased their scores on both the mechanical withdrawal test and the tail flick test; in an acute plantar incision model, scores were also significantly increased on the mechanical withdrawal test at 4 and 5 days after the operation. Moreover, pseudo-germ-free mice receiving fecal bacteria from patients with CIPA took significantly longer to escape and had a significantly longer path length on training days 1, 2, and 5 and also had fewer platform crossings and spent less time in the target quadrant in the probe trial. CONCLUSIONS: Our results suggest that the gut microbiota in CIPA subjects plays a key role in behaviors. Therapeutic strategies for improving the gut microbiota might alleviate CIPA symptoms.

7.
Int J Biol Macromol ; 187: 939-954, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34343588

RESUMO

This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying. Four formulations of microencapsulation of Pulicaria jaubertii extract (MPJE) were produced, including WPGA-MPJE, SCGA-MPJE, SPGA-MPJE, and GA-MPJE. The formulations were stored at 4 °C and 25 °C for 28 days to assess the storage stability. The results indicated that mixtures of proteins with GA improved the physicochemical properties and bioactive content of the MPJE compared to GA-MPJE. The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C. The microstructure of MPJE formulations showed a flat surface without any visible cracking on surfaces. The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.

8.
Infect Genet Evol ; 95: 105053, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34455089

RESUMO

New human immunodeficiency virus (HIV)-1 circulating recombinant forms (CRFs) have recently emerged and disseminated rapidly in China; in total 38 CRFs have been identified thus far. Yunnan province shares its border with Myanmar, and is regarded as a "hotspot" for the occurrence of new HIV-1 recombinations; more than half of novel CRFs reported in China have been first documented in Yunnan province. In the present study, based on the information available on four existing near-full-length genome (NFLG) sequences, combined with data on four other closely related sequences obtained via Basic Local Alignment Search Tool (BLAST) analysis, NFLG/subregion phylogenetic, bootscanning, and time to the most recent common ancestor (TMRCA) analyses were performed. Phylogenetic analysis revealed that the eight strains demonstrated the formation of a distinct monophyletic branch with a bootstrap value of 100%. Strains in this branch were distantly related to all known HIV-1 CRFs; it was temporarily named CRF111_01C. Bootscanning analysis revealed that CRF111_01C consisted of a CRF01_AE backbone and four inserted subtype C segments. Remarkably, CRF111_01C shared six mosaic fragment identities with the previously identified CRF100_01C. Furthermore, CRF111_01C may be deemed a potential second-generation CRF consisting of CRF100_01C and C. Coalescent Bayesian analyses revealed that the TMRCA of CRF111_01C was approximately the period 1999-2002. The emergence of such second-generation recombinants highlights that continuous molecular screening is necessary to carefully monitor the evolutionary dynamics of HIV-1 epidemics.

9.
J Nat Prod ; 84(8): 2366-2373, 2021 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-34445872

RESUMO

Structurally diverse tigliane diterpenoids have drawn significant research interest for drug discovery over many decades. Using LC-MS-guided fractionation and separation, the first phytochemical investigation on Wikstroemia lamatsoensis led to the isolation of eight tiglianes (1-8), including two new compounds, wikstrocin D (1) and wikstrocin E (2). The new structures were elucidated based on extensive physicochemical and spectroscopic analyses. The characteristic ESIMS/MS fragmentations of tiglianes 1-8 were also summarized. Among the isolated tiglianes, three compounds (8, 5, and 7) showed the most potent anti-HIV activity, with IC50 values of 0.18, 3.8, and 12.8 nM, respectively.

10.
Plants (Basel) ; 10(8)2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34451729

RESUMO

Three undescribed 8,3'-neolignans, corynol (1), 3-methoxy-corynol (2) and 3'-deoxy-corynol (3), together with two bergenin derivatives, three flavonoids, two hydrolysable tannins and six simple phenolic compounds, were isolated from the twigs of Corylopsis coreana Uyeki. The structures of the 8,3'-neolignans were elucidated by analyzing their NMR, HRESIMS and ECD spectra. All the isolated compounds were evaluated for their SIRT1 stimulatory activity, and 3'-deoxy-corynol (3) showed SIRT1 stimulation activity. Furthermore, a docking study of 3 was performed with three representative binding pockets of SIRT1.

11.
Animals (Basel) ; 11(7)2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34359169

RESUMO

This study was to investigate the effects of Bacillus subtilis on production performance and bone pathophysiological characteristics of layers. Twenty-four 48-week-old Lohmann Pink-shell laying hens were randomly divided into two groups: a basic diet (control) and the basic diet mixed with Bacillus subtilis (0.5 g/kg) for a 60-day trial. Statistically, independent-sample t-test was used to assess the treatment differences. The results showed that Bacillus subtilis supplementation improved the percent of marketable eggs (p < 0.05) with reduced numbers of broken and soft-shelled eggs but had no effects on egg weight, height of albumen, yolk color, and Haugh unit (p > 0.05). Bacillus subtilis supplement also elevated maximum load (p = 0.06), maximum stress (p = 0.01), stiffness (p < 0.01), and Young's modulus (p < 0.01) but suppressed maximum strain (p = 0.06) in the femur. In addition, compared with control birds, phosphorous concentration (p < 0.01) was reduced in serum at day 61 but increased in the femur (p < 0.05) in Bacillus subtilis fed birds. Bacillus subtilis fed birds also had lower magnesium concentrations in both femur (p = 0.04) and feces (p = 0.09). Furthermore, Bacillus subtilis increased plasma estrogen concentration (p = 0.01) and femur TNF receptor superfamily member 11b (OPG) expression (p < 0.05) but reduced plasma IL-1 (p < 0.01) and TNF-α (p < 0.01) concentrations. These results indicate that Bacillus subtilis could be used as a health promotor to reduce overproduction-induced inflammation and associated bone damage and to increase marketable egg production. The data provide evidence for developing a management strategy to use Bacillus subtilis as a feed additive to improve marketable egg production and health and welfare status of laying hens.

12.
Int J Mol Sci ; 22(14)2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34298948

RESUMO

Verticillium wilt, caused by Verticillium dahliae, is a devastating disease for many important crops, including cotton. Kiwellins (KWLs), a group of cysteine-rich proteins synthesized in many plants, have been shown to be involved in response to various phytopathogens. To evaluate genes for their function in resistance to Verticillium wilt, we investigated KWL homologs in cotton. Thirty-five KWL genes (GhKWLs) were identified from the genome of upland cotton (Gossypium hirsutum). Among them, GhKWL1 was shown to be localized in nucleus and cytosol, and its gene expression is induced by the infection of V. dahliae. We revealed that GhKWL1 was a positive regulator of GhERF105. Silencing of GhKWL1 resulted in a decrease, whereas overexpression led to an increase in resistance of transgenic plants to Verticillium wilt. Interestingly, through binding to GhKWL1, the pathogenic effector protein VdISC1 produced by V. dahliae could impair the defense response mediated by GhKWL1. Therefore, our study suggests there is a GhKWL1-mediated defense response in cotton, which can be hijacked by V. dahliae through the interaction of VdISC1 with GhKWL1.


Assuntos
Ascomicetos , Proteínas Fúngicas , Regulação Fúngica da Expressão Gênica , Gossypium , Doenças das Plantas , Fatores de Transcrição , Regulação para Cima , Fatores de Virulência , Ascomicetos/genética , Ascomicetos/metabolismo , Ascomicetos/patogenicidade , Proteínas Fúngicas/biossíntese , Proteínas Fúngicas/genética , Gossypium/genética , Gossypium/metabolismo , Gossypium/microbiologia , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Fatores de Transcrição/biossíntese , Fatores de Transcrição/genética , Fatores de Virulência/biossíntese , Fatores de Virulência/genética
13.
Genome ; : 1-11, 2021 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-34253086

RESUMO

Cotton (Gossypium L.) is the most important fiber crop worldwide. Here, transcriptome analysis was conducted on developing fibers of a G. mustelinum introgression line, IL9, and its recurrent parent, PD94042, at 17 and 21 days post-anthesis (dpa). Differentially expressed genes (DEGs) of PD94042 and IL9 were identified. Gene Ontology (GO) enrichment analysis showed that the annotated DEGs were rich in two main biological processes and two main molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis likewise showed that the annotated DEGs were mainly enriched in metabolic pathways and biosynthesis of secondary metabolites. In total, 52 DEGs were selected as candidate genes based on comparison of the DEGs and GO function annotation information. Quantitative real-time PCR (RT-qPCR) analysis results for 12 randomly selected DEGs were consistent with transcriptome analysis. SNP identification based on G. mustelinum chromatin segment introgression showed that 394 SNPs were identified in 268 DEGs, and two genes with known functions were identified within fiber strength quantitative trait loci (QTL) regions or near the confidence intervals. We identified 52 key genes potentially related to high fiber strength in a G. mustelinum introgression line and provided significant insights into the study of cotton fiber quality improvement.

14.
J Nat Med ; 75(4): 1058-1066, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34287744

RESUMO

Macrocyclic daphnane orthoesters (MDOs) have attracted significant research interest for the drug discovery to cure HIV infection based on the "Shock and Kill" strategy. In the present study, the first chemical study on Wikstroemia ligustrina (Thymelaeaceae) was carried out by LC-MS analysis and phytochemical investigation. Nine daphnane diterpenoids (1-9) including seven MDOs were detected by LC-MS analysis. Further phytochemical investigation resulted in the isolation and structural elucidation of five daphnanes (1, 2, 5, 8, and 9) with potent anti-HIV activity. Taking the isolated MDO (1) as a model compound, the MS/MS fragmentation pathway was also elucidated.

15.
Sci Data ; 8(1): 161, 2021 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-34183677

RESUMO

To better understand the psychological and physiological basis of human emotion, increasing interest has been drawn towards ambulatory recordings of emotion-related data beyond the laboratories. By employing smartphones-based ambulatory assessment and wrist-worn physiological recording devices, the Daily Ambulatory Psychological and Physiological recording for Emotion Research (DAPPER) dataset provides momentary self-reports and physiological data of people's emotional experiences in their daily life. The dataset consists of ambulatory psychological recordings from 142 participants and physiological recordings from 88 of them over five days. Both the experience sampling method (ESM) and the day reconstruction method (DRM) were employed to have a comprehensive description of the participants' daily emotional experiences. Heart rate, galvanic skin response, and three-axis acceleration were recorded during the day time. By including multiple types of physiological and self-report data at a scale of five days with 100+ participants, the present dataset is expected to promote emotion researches in real-life, daily settings.

16.
J Food Sci ; 86(7): 2990-3000, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34146421

RESUMO

Effects of infrared ray roasting (IRR) on the oxidation stability and flavors of virgin rapeseed oil (VROs) at 110-170°C were investigated and compared with traditional roller roasting (TRR). Results showed that IRR samples showed lower acid and peroxides values, higher oxidation stability index, and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity than TRR ones. IRR samples displayed better thermal expansion of rapeseed for internal fragmentation from microstructures, which facilitated the release of tocophenols (652.63-748.78 mg/kg) and 4-vinylsyringol (7.54-678.19 mg/kg), compared with TRR ones with tocophenols (652.63-689.28 mg/kg) and 4-vinylsyringol (7.54-524.18 mg/kg) contributing to better oxidation stability. Moreover, important volatile compounds, including pyrazines, isothiocyanates, nitriles and aldehydes, were formed quantitatively more in IRR than TRR samples, which was attributed to better heat transfer efficiency and internal fragmentation promoting complex reactions inside rapeseed. Therefore, IRR has more positive roasting effects on VROs than TRR. PRACTICAL APPLICATION: Virgin rapeseed oil is a massively consumed flavor vegetable oil, but the traditional high-temperature roller seed roasting process can cause serious quality problems. Our work applied a novel roasting technology, infrared ray roasting to rapeseed pretreatment. The results show that this new type of roasting technology is more efficient and stable and has important applications in the production of virgin rapeseed oil with better oxidative stability and flavor.


Assuntos
Brassica napus/química , Aromatizantes/química , Manipulação de Alimentos/métodos , Óleo de Brassica napus/química , Sementes/efeitos da radiação , Brassica napus/efeitos da radiação , Culinária , Aromatizantes/efeitos da radiação , Manipulação de Alimentos/instrumentação , Temperatura Alta , Raios Infravermelhos , Oxirredução , Sementes/química
17.
Phytochemistry ; 189: 112816, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34087503

RESUMO

Five undescribed sterol derivatives, (22E,24R)-7α-methoxy-5α,6α-epoxyergosta-8(14),22-diene-3ß,15ß-diol, (22E,24R)-5α,6α-epoxyergosta-8(14),22-diene-3ß,7ß,15α-triol, (22E,24R)-3ß,5α-dihydroxy-14ß,15ß-epoxyergosta-7,22-diene-6-one, (22E,24R)-6α-methoxy-7α,15ß-dihydroxyergosta-4,8(14),22-triene-3-one, and (25S)-ergosta-7,24(28)-diene-3ß,4α,6α,26-tetraol were isolated from the extract of Talaromyces stipitatus, along with eight known congeners. This is the first example of a class of ergosterols isolated from T. stipitatus. Their structures with absolute configurations were elucidated based on NMR spectroscopic data, ECD calculations, and X-ray crystallographic analyses. All these compounds were tested for their effects on three hepatoma cell lines including Hep3B, HepG2, and Huh-7. Moreover, (22E,24R)-5α,6α-epoxyergosta-8(14),22-diene-3ß,7ß,15α-triol and (22E,24R)-9α,15α-dihydroxyergosta-4,6,8(14),22-tetraen-3-one were further evaluated for their impacts on cell cycle progression and apoptosis due to their pronounced cytotoxicity, to uncover their underlying mechanisms. Our results suggested that their antiproliferative activities were mainly mediated by inducing cell apoptosis.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Talaromyces , Carcinoma Hepatocelular/tratamento farmacológico , Linhagem Celular , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Estrutura Molecular , Esteroides/farmacologia
18.
J Air Waste Manag Assoc ; 71(9): 1175-1184, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34061727

RESUMO

Montan resin (MR) is an industrial by-product or solid waste generated during the production of refined montan wax and is not typically reused. In this paper, a bio-modification method using three strains of microorganisms, Acinetobacter venetianus (AV), Pseudomonas aeruginosa (PA), and Phanerochaete chrysosporium (PC), was studied to promote the performance and bio-function of MR so that MR could be recycled. MR can be degraded by these three microorganisms, and their weight loss rates were similar over the treatment period of 15 days. Compared with the original MR, the hydrophilicity of modified MRs was improved, which was related to the increase in apparent oil-water partition coefficients (Kows) and oxygen-containing and hydrophilic groups in modified MRs based on IR and GC-MS analysis. The bio-function of modified MRs by the three strains in terms of promoting maize seed germination and seedling growth was greater compared with untreated MR. Overall, these findings indicate that biomodified MRs might have useful agriculture applications.Implications: An environmentally-friendly method using microorganisms to achieve recycle of solid waste, montan resin (MR) was established in this study. Through this bio-treatment, the performance and bio-function of MR were both improved, that is the appearance and hydrophilicity of modified MRs were better than thoes in before, and the modified MRs treated by three strains showed the better promoting effects on maize seed germination and seedling growth than untreated MR, indicating the modified MRs have the certain potential of agricultural utilization in the future.

19.
Signal Transduct Target Ther ; 6(1): 217, 2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34103473

RESUMO

We examined the safety and efficacy of human umbilical cord mesenchymal stem cell (hUC-MSC) infusion for immune non-responder (INR) patients with chronic HIV-1 infection, who represent an unmet medical need even in the era of efficient antiretroviral therapy (ART). Seventy-two INR patients with HIV were enrolled in this phase II randomized, double-blinded, multicenter, placebo-controlled, dose-determination trial (NCT01213186) from May 2013 to March 2016. They were assigned to receive high-dose (1.5 × 106/kg body weight) or low-dose (0.5 × 106/kg body weight) hUC-MSC, or placebo. Their clinical and immunological parameters were monitored during the 96-week follow-up study. We found that hUC-MSC treatment was safe and well-tolerated. Compared with baseline, there was a statistical increase in CD4+ T counts in the high-dose (P < 0.001) and low-dose (P < 0.001) groups after 48-week treatment, but no change was observed in the control group. Kaplan-Meier analysis revealed a higher cumulative probability of achieving an immunological response in the low-dose group compared with the control group (95.8% vs. 70.8%, P = 0.004). However, no significant changes in CD4/CD8+ T counts and CD4/CD8 ratios were observed among the three groups. In summary, hUC-MSC treatment is safe. However, the therapeutic efficacy of hUC-MSC treatment to improve the immune reconstitution in INR patients still needs to be further investigated in a large cohort study.

20.
Oral Dis ; 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-34061424

RESUMO

OBJECTIVE: This study was designed to identify the role of circRNAs in tongue squamous cell carcinoma (TSCC) and discover novel circRNAs as potential diagnostic or therapeutic targets for TSCC. RESULTS: The circRNA expression profiles in TSCC were evaluated by high-throughput sequencing in 6 TSCC patients. Our data showed that 69 circRNAs were downregulated and 208 were upregulated significantly (fold change ≥2.0; p < 0.05). GO and KEGG analysis demonstrated that the parental genes of differentially expressed circRNAs were potentially implicated in TSCC pathogenesis. After bioinformatics analysis, 4 circRNAs (hsa_circ_0005035, hsa_circ_0002360, hsa_circ_0066251, and hsa_circ_0003161) were selected and successfully validated by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) in 40 patients and 3 TSCC cell lines. The establishment and analysis of the circRNA-miRNA-mRNA network revealed the potential function and mechanism of these candidate circRNAs. CONCLUSION: Our study provided a comprehensive circRNAs expression profile of TSCC by RNA-seq and discovered 4 novel circRNAs with potential great diagnostic and therapeutic value. These findings provide new insights into the development of potential biomarkers and targets for TSCC treatment.

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