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1.
Nanotechnology ; 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34598166

RESUMO

Herein, we have reported a facile one-pot strategy to synthesize fluorescent Cu nanclusters (Cu NCs) by using ʟ-histidine as stabilizer, and ascorbic acid (AA) as reducing agent. Cu NCs are stable, water-dispersible, and emit bright cyan emission with a quantum yield 26.08%. The Cu NCs can be employed as colorimetric and fluorimetric dual-mode detector, exhibiting excellent selectivity and sensitivity for detecting 2, 4-dinitrophenol (DNP) specifically. Notably, Cu NCs were a sensitive sensor, which had speciality to detect DNP in range of linearity from 0.01 to 0.15 mM with a discernable limit as low as 3.96 µM. The mechanism of efficiently selective detection of DNP by Cu NCs was analyzed by UV absorption, fluorescence decay spectrum, and the performance of "turn off" towards DNP was determined as the inner filter effect (IFE) and static quenching effect (SQE). Further, the environmental tolerance of the Cu NCs probe was estimated by using the different natural water samples, demonstrating its great promise in the field of DNP monitoring and water sample analysis.

2.
J Adv Res ; 33: 153-165, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34603786

RESUMO

Introduction: The development and prognosis of HCC involve complex molecular mechanisms, which affect the effectiveness of its treatment strategies. Tumor mutational burden (TMB) is related to the efficacy of immunotherapy, but the prognostic role of TMB-related genes in HCC has not yet been determined clearly. Objectives: In this study, we identified TMB-specific genes with good prognostic value to build diagnostic and prognostic models and provide guidance for the treatment of HCC patients. Methods: Weighted gene co-expression network analysis (WGCNA) was applied to identify the TMB-specific genes. And LASSO method and Cox regression were used in establishing the prognostic model. Results: The prognostic model based on SMG5 and MRPL9 showed patients with higher prognostic risk had a remarkedly poorer survival probability than their counterparts with lower prognostic risk in both a TCGA cohort (P < 0.001, HR = 1.93) and an ICGC cohort (P < 0.001, HR = 3.58). In addition, higher infiltrating fractions of memory B cells, M0 macrophages, neutrophils, activated memory CD4 + T cells, follicular helper T cells and regulatory T cells and higher expression of B7H3, CTLA4, PD1, and TIM3 were present in the high-risk group than in the low-risk group (P < 0.05). Patients with high prognostic risk had higher resistance to some chemotherapy and targeted drugs, such as methotrexate, vinblastine and erlotinib, than those with low prognostic risk (P < 0.05). And a diagnostic model considering two genes was able to accurately distinguish patients with HCC from normal samples and those with dysplastic nodules. In addition, knockdown of SMG5 and MRPL9 was determined to significantly inhibit cell proliferation and migration in HCC. Conclusion: Our study helps to select patients suitable for chemotherapy, targeted drugs and immunotherapy and provide new ideas for developing treatment strategies to improve disease outcomes in HCC patients.

3.
World J Gastrointest Oncol ; 13(9): 1184-1195, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34616522

RESUMO

BACKGROUND: It remains controversial as to which pathological classification is most valuable in predicting the overall survival (OS) of patients with gastric cancer (GC). AIM: To assess the prognostic performances of three pathological classifications in GC and develop a novel prognostic nomogram for individually predicting OS. METHODS: Patients were identified from the Surveillance, Epidemiology, and End Results program. Univariate and multivariate analyses were performed to identify the independent prognostic factors. Model discrimination and model fitting were evaluated by receiver operating characteristic curves and Akaike information criteria. Decision curve analysis was performed to assess clinical usefulness. The independent prognostic factors identified by multivariate analysis were further applied to develop a novel prognostic nomogram. RESULTS: A total of 2718 eligible GC patients were identified. The modified Lauren classification was identified as one of the independent prognostic factors for OS. It showed superior model discriminative ability and model-fitting performance over the other pathological classifications, and similar results were obtained in various patient settings. In addition, it showed superior net benefits over the Lauren classification and tumor differentiation grade in predicting 3- and 5-year OS. A novel prognostic nomogram incorporating the modified Lauren classification showed superior model discriminative ability, model-fitting performance, and net benefits over the American Joint Committee on Cancer 8th edition tumor-node-metastasis classification. CONCLUSION: The modified Lauren classification shows superior net benefits over the Lauren classification and tumor differentiation grade in predicting OS. A novel prognostic nomogram incorporating the modified Lauren classification shows good model discriminative ability, model-fitting performance, and net benefits.

4.
Stem Cells Dev ; 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34549601

RESUMO

Urine-derived stem cells (USCs) are adult stem cells isolated from urine with strong proliferative ability and differentiation potentials. Cell transplantation of USCs could partly repair liver injury. It has been reported that the proliferative ability of bone mesenchymal stem cells in patients with chronic liver failure is significantly lower than in patients without liver disease. The aim of this study was therefore to evaluate the biological characteristics of USCs from end-stage liver disease patients (LD-USCs) compared to those from normal healthy individuals (N-USCs), with a view to determining whether autologous USCs can be applied to the treatment of liver disease. In this study USCs were isolated from urine samples of male patients with end-stage liver disease. Adherent USCs exhibit a spindle- or rice grain-like morphology, and express CD24, CD29, CD73, CD90, and CD146 surface markers, but not CD31, CD34, CD45, and CD105. We observed no differences in cell morphology or cell surface marker profile between LD-USCs and N-USCs. LD-USCs exhibited similar proliferative, colony-forming, apoptotic, and migratory abilities to N-USCs. Both USCs demonstrated similar capacities for osteogenic, adipogenic, and chondrogenic differentiation. When USCs were transplanted into CCl4 treatment-induced acute and chronic liver fibrosis mouse models, we observed a decrease in liver index, recovery of ALT and AST levels, alleviation of liver tissue injury, and dramatic improvement of liver tissue structure. USC transplantation can effectively recover liver function and improve liver tissue damage in acute or chronic liver injury mouse models. According to the results, we concluded that the biological characteristics of LD-USCs are not affected by basic liver disease. This study provides further evidence of the stem cell characteristics and liver repair function of LD-USCs, which may serve as a theoretical and experimental foundation for autologous USC transplantation technology in the treatment of liver failure and end-stage liver diseases.

5.
BMC Plant Biol ; 21(1): 395, 2021 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-34425748

RESUMO

BACKGROUND: The identification and functional analysis of genes that improve tolerance to low potassium stress in S. spontaneum is crucial for breeding sugarcane cultivars with efficient potassium utilization. Calcineurin B-like (CBL) protein is a calcium sensor that interacts with specific CBL-interacting protein kinases (CIPKs) upon plants' exposure to various abiotic stresses. RESULTS: In this study, nine CBL genes were identified from S. spontaneum. Phylogenetic analysis of 113 CBLs from 13 representative plants showed gene expansion and strong purifying selection in the CBL family. Analysis of CBL expression patterns revealed that SsCBL01 was the most commonly expressed gene in various tissues at different developmental stages. Expression analysis of SsCBLs under low K+ stress indicated that potassium deficiency moderately altered the transcription of SsCBLs. Subcellular localization showed that SsCBL01 is a plasma membrane protein and heterologous expression in yeast suggested that, while SsCBL01 alone could not absorb K+, it positively regulated K+ absorption mediated by the potassium transporter SsHAK1. CONCLUSIONS: This study provided insights into the evolution of the CBL gene family and preliminarily demonstrated that the plasma membrane protein SsCBL01 was involved in the response to low K+ stress in S. spontaneum.


Assuntos
Calcineurina/genética , Genoma de Planta , Filogenia , Imunidade Vegetal/genética , Proteínas de Plantas/genética , Deficiência de Potássio/genética , Saccharum/genética , Membrana Celular , Produtos Agrícolas/genética , Evolução Molecular , Regulação da Expressão Gênica de Plantas , Variação Genética , Análise de Sequência de Proteína
6.
Nat Commun ; 12(1): 4876, 2021 08 12.
Artigo em Inglês | MEDLINE | ID: mdl-34385436

RESUMO

While the printed circuit board (PCB) has been widely considered as the building block of integrated electronics, the world is switching to pursue new ways of merging integrated electronic circuits with textiles to create flexible and wearable devices. Herein, as an alternative for PCB, we described a non-printed integrated-circuit textile (NIT) for biomedical and theranostic application via a weaving method. All the devices are built as fibers or interlaced nodes and woven into a deformable textile integrated circuit. Built on an electrochemical gating principle, the fiber-woven-type transistors exhibit superior bending or stretching robustness, and were woven as a textile logical computing module to distinguish different emergencies. A fiber-type sweat sensor was woven with strain and light sensors fibers for simultaneously monitoring body health and the environment. With a photo-rechargeable energy textile based on a detailed power consumption analysis, the woven circuit textile is completely self-powered and capable of both wireless biomedical monitoring and early warning. The NIT could be used as a 24/7 private AI "nurse" for routine healthcare, diabetes monitoring, or emergencies such as hypoglycemia, metabolic alkalosis, and even COVID-19 patient care, a potential future on-body AI hardware and possibly a forerunner to fabric-like computers.


Assuntos
Técnicas Biossensoriais/instrumentação , Medicina de Precisão/instrumentação , Têxteis , Dispositivos Eletrônicos Vestíveis , Tecnologia sem Fio/instrumentação , Técnicas Biossensoriais/métodos , COVID-19/diagnóstico , COVID-19/prevenção & controle , COVID-19/virologia , Desenho de Equipamento , Humanos , Monitorização Fisiológica/instrumentação , Monitorização Fisiológica/métodos , Medicina de Precisão/métodos , SARS-CoV-2/fisiologia , Suor/fisiologia
7.
Environ Pollut ; 288: 117633, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34247004

RESUMO

The crop-livestock system is responsible for a large proportion of global reactive nitrogen (Nr) losses, especially from China. There are diverse livestock systems with contrasting differences in feed, livestock and manure management. However, it is not yet well understood which factors greatly impact on the nitrogen (N) budgets and losses of each system. In this study, we systematically evaluated the N budgets of the crop-livestock production system from 1980 to 2050 in China by identifying the differences of 20 distinct livestock systems. During 1980 to 2010, the total N flow through the crop-livestock system increased from 21.4 to 49.7 Tg, with large variations in different input/output pathways, due to the strong livestock transitions of production towards to a monogastric and landless industrial system. Different systems contributed differently to the total N budgets in 2010. For example, the landless industrial system contributed 67% of livestock product N output, but accounted for 80% of total mineral N fertilizer use and feed N imports by the whole crop-livestock system. The mixed system had the highest rate of N use efficiency at system level due to high dependence on recycled N. N losses were diversely distributed by different systems, with the mixed ruminant system responsible for the majority of NH3-N emission in livestock production, and the grazing ruminant system dominant in NO3-N losses in feed production. The total N entering the crop-livestock system is estimated to be 53.9 Tg with total N losses of 41.3 Tg in 2050 under a business-as-usual scenario. However, this amount could be significantly decreased through combined measures that indicate a considerable potential for future improvements. Overall, our results provide new insights into N use and the management of livestock production.


Assuntos
Gado , Nitrogênio , Agricultura , Animais , China , Fertilizantes , Indústrias , Esterco
8.
Br J Nutr ; : 1-10, 2021 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-34308819

RESUMO

DNA methylation is one of the most important epigenetic modifications in breast cancer (BC) development, and long-term dietary habits can alter DNA methylation. Cadherin-4 (CDH4, a member of the cadherin family) encodes Ca2+-dependent cell-cell adhesion glycoproteins. We conducted a case-control study (380 newly diagnosed BC and 439 cancer-free controls) to explore the relationship of CDH4 methylation in peripheral blood leukocyte DNA (PBL DNA), as well as its combined and interactive effects with dietary factors on BC risk. A case-only study (335 newly diagnosed BC) was conducted to analyse the association between CDH4 methylation in breast tissue DNA and dietary factors. CDH4 methylation was detected using quantitative methylation-specific PCR. Unconditional logistic regressions were used to analyse the association of CDH4 methylation in PBL DNA and BC risk. Cross-over analysis and unconditional logistic regression were used to calculate the combined and interactive effects between CDH4 methylation in PBL DNA and dietary factors in BC. CDH4 hypermethylation was significantly associated with increased BC risk in PBL DNA (ORadjusted (ORadj) = 2·70, (95 % CI 1·90, 3·83), P < 0·001). CDH4 hypermethylation also showed significant combined effects with the consumption of vegetables (ORadj = 4·33, (95 % CI 2·63, 7·10)), allium vegetables (ORadj = 7·00, (95 % CI 4·17, 11·77)), fish (ORadj = 7·92, (95 % CI 3·79, 16·53)), milk (ORadj = 6·30, (95 % CI 3·41, 11·66)), overnight food (ORadj = 4·63, (95 % CI 2·69, 7·99)), pork (ORadj = 5·59, (95 % CI 2·94, 10·62)) and physical activity (ORadj = 4·72, (95 % CI 2·87, 7·76)). Moreover, consuming milk was significantly related with decreased risk of CDH4 methylation (OR = 0·61, (95 % CI 0·38, 0·99)) in breast tissue. Our findings may provide direct guidance on the dietary intake for specific methylated carriers to decrease their risk for developing BC.

9.
Materials (Basel) ; 14(13)2021 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-34201910

RESUMO

It is of great significance to realize the sustainable development of the environment to synthesize functional materials by value-added utilization of waste resources. Herein, a composite material of polyacrylic acid/lignosulfonate sodium/cotton biochar (PAA/LS/BC) was successfully prepared by grafting polyacrylic acid with functionalized waste cotton biochar and lignosulfonate sodium. The obtained absorbent showed prominent capture ability toward Pb2+ and methylene blue (MB) with capture characteristics of the pseudo-second-order model and Langmuir isotherm model. This experiment explored the adsorption performance of the adsorbent for pollutants at different conditions, and further revealed the selective adsorption of Pb2+ and MB in the mixed system. Analysis confirmed that electrostatic attraction and complexation are the most critical methods to remove contaminants. Additionally, the regeneration and stability experiment showed that the adsorption capacity of PAA/LS/BC for pollutants did not significantly decrease after five runs of adsorption-desorption. Various results can demonstrate that the adsorbent has excellent performance for removing pollutants and can be used as a material with development potential in the field of adsorption.

10.
J Pain Res ; 14: 2083-2093, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34267552

RESUMO

Background and Objective: Neuropathic pain remains a clinical challenge with limited effective treatments. Previous studies have found that magnolol (Mag), an ingredient existing in some herbs, showed neuroprotective effect. However, it remains unclear whether Mag can alleviate neuropathic pain. Methods: Chronic constriction injury (CCI) is used as the neuropathic pain model. Mice were randomly divided into 5 groups: Sham, CCI, CCI + 5, 10, 30 mg/kg Mag groups. Thermal and mechanical paw withdrawal threshold were performed at baseline and on the 3rd, 5th, 7th, 14th days post-surgery. Lumbar spinal cord and blood samples were collected on the 14th day. Blood lipid profile, kidney and liver functions, as well as the activation of microglia were evaluated, along with the related signal pathway examined using multiple methods including immunohistochemistry, RT-PCR and Western blot. Results: Mag alleviated thermal and mechanical hypersensitivity in CCI mice. CCI activated microglia and upregulated the expression of P2Y12, while Mag inhibited microglial activation, and downregulated the expression of P2Y12. Mag also blocked the activation of p38 mitogen-activated protein kinase (MAPK) and other pain-related cytokines such as IL-6, TNF-α and IL-1ß. Conclusion: The findings indicate that Mag has antinociceptive effect on neuropathic pain, probably mediated through P2Y12 receptors and p38 MAPK mediated pathways. With its relatively safe profile, Mag may be a potential therapeutic agent for neuropathic pain.

11.
Environ Pollut ; 287: 117623, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34171728

RESUMO

China has effectively reduced the fine particulate (PM2.5) pollution from 2015 to 2020. Ozone pollution and related health impacts have become severe contemporaneously. The coordinated control of PM2.5 and ozone is becoming a new issue for China's air pollution control. This study quantitatively assessed the health impacts attributed to PM2.5 and ozone pollution in 338 Chinese cities from 2015 to 2020 and estimated the possible health benefits from achieving dual concentration targets during 2021-2025. Results show PM2.5 caused a total health impact of 2.45 × 107 disability-adjusted life years (DALYs) in 2020. All-cause and respiratory ozone-related health impact in 2020 was 1.04 × 107 DALYs and 1.56 × 106 DALYs. Between 2015 and 2020, the PM2.5-related health impacts decreased by 14.97%, while those ozone-related increased by 94.61% and 96.54% for all-cause and respiratory. Cities in the North China Plain have suffered higher health impacts attributable to PM2.5 and ozone pollution, indicating that the two-pollutant coordinated control is primarily needed. By achieving aggressive concentration target (decreasing 10%) between 2020 and 2025, China will reduce the PM2.5-related health impacts in 338 cities by 1.56 × 106 DALYs (improving 6.37%). By achieving general target (decreasing 10% or within the Interim target-1 of World Health Organization), the PM2.5-related health benefit will be 7.98 × 105 DALYs (improving 3.25%). The deteriorating ozone health risks will also be improved. Controlling air pollution in large cities and regional center cities can achieve remarkable health benefits. Due to the inter-region, inter-province, and inter-city difference of health impacts, targeted and differentiated pollution prevention and control need to be implemented.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Ozônio , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China , Cidades , Ozônio/análise , Material Particulado/análise
12.
J Hazard Mater ; 419: 126385, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34175705

RESUMO

Solar cells based on crystalline silicon wafers have dominated the global photovoltaic market for many years. Unfortunately, a large amount of photovoltaic silicon waste (PSW) also was produced during the process of cutting silicon ingot into silicon wafer. The improperly discarded PSW will bring about serious environmental hazardous problems, so it is highly necessary to safely and effectively recover and utilize PSW. Here, we report self-assembled 3D Si3N4@SiO2 nanofibre sponges utilising PSW as silicon sources for the first time. This kind of ceramic sponge displays excellent compression resilience under a maximum strain of 67% due to the flexibility of the Si3N4@SiO2 nanofibres. The Si3N4@SiO2 nanofibre sponges can withstand high temperatures beyond 1200 °C with negligible weight loss and demonstrates favourable thermal insulation properties. Furthermore, the porous Si3N4@SiO2 nanofibre sponges possess ultra-low dielectric properties, with the minimum dielectric constant and dielectric loss approaching 1 and 0, respectively. In short, a simple and low-cost technology using industrial waste to fabricate versatile Si3N4@SiO2 nanofibre sponges with prominent performance is of great significance for the development and application of 3D ceramic architectures in various industry fields including aerospace, electronic devices and thermal insulation.

13.
Chem Commun (Camb) ; 57(48): 5985, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-34095918

RESUMO

Retraction of 'Chemical synthesis and antigenic activity of a phosphatidylinositol mannoside epitope from Mycobacterium tuberculosis' by Shi-Yuan Zhao et al., Chem. Commun., 2020, 56, 14067-14070, DOI: 10.1039/D0CC05573E.

14.
Am J Transl Res ; 13(5): 4573-4580, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34150037

RESUMO

OBJECTIVE: This study aimed to investigate the plasma cytokine changes and its clinical significance in intracranial infection secondary to traumatic brain injury. METHODS: A total of 60 cases with intracranial infection secondary to traumatic brain injury admitted to our hospital from January 2017 to December 2019 were selected as the research objects, of whom, 24 cases with mild infection, 20 with moderate infection, and 16 with severe infection. Another 60 cases without intracranial infection secondary to traumatic brain injury during the same period were selected as the uninfected group. A comparison of infected and uninfected groups on changes of plasma cytokines (IL-1ß, IL-2, IL-6, IL-8, TNF-α, IFN-γ) and high mobility group-1 protein (HMGB1) were conducted to analyze the correlation between plasma cytokines and disease severity. RESULTS: The data of IL-1ß, IL-2, IL-6, IL-8, TNF-α, IFN-γ and HMGB1 levels in both groups on day 1, day 3 and day 5 after the surgery were obtained. The results indicated that for infected group, the differences were significant among these 3 days (P<0.05), and the data on day 5 were all higher than that on day 1 and day 3 (P<0.05). While for uninfected group, there was no significant difference among those 3 days (P>0.05). The differences in different severity of infection on day 5 showed statistically significance (P<0.05), and it was positively correlated with the severity (P<0.05). CONCLUSION: The cytokines content in intracranial infection secondary to traumatic brain injury increased significantly, which was closely related to the severity of the infection. These factors can be used as monitoring indicators for diagnosis of intracranial infection and assessment ofthe severity.

15.
Cell Death Dis ; 12(5): 428, 2021 04 30.
Artigo em Inglês | MEDLINE | ID: mdl-33931579

RESUMO

ADAMTS9 belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family, and its expression is frequently silenced due to promoter hypermethylation in various human cancers. However, the underlying mechanisms remain largely unknown. In this study, we investigated the inhibitory effects of ADAMTS9 on gastric cancer (GC) cells. We initially examined ADAMTS9 protein level in 135 GC and adjacent normal tissue pairs, showing that ADAMTS9 was strikingly decreased in the malignant specimens and patients with low ADAMTS9 expression exhibited more malignant phenotypes and poorer outcome. ADAMTS9 expression was restored in AGS and BGC-823 cells, which then markedly suppressed cellular viability and motility in vitro and in vivo. As ADAMTS9 was enriched in the nuclei of gastric mucosal cells, RNA-sequencing experiment showed that ADAMTS9 significantly altered gene expression profile in BGC-823 cells. Additionally, DNA methyltransferase 3α (DNMT3A) was identified to be responsible for the hypermethylation of ADAMTS9 promoter, and this methyltransferase was ubiquitinated by ring finger protein 180 (RNF180) and then subject to proteasome-mediated degradation. In conclusion, we uncovered RNF180/DNMT3A/ADAMTS9 axis in GC cells and showed how the signaling pathway affected GC cells.


Assuntos
Proteína ADAMTS9/metabolismo , DNA (Citosina-5-)-Metiltransferases/metabolismo , Neoplasias Gástricas/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , Proteína ADAMTS9/biossíntese , Proteína ADAMTS9/genética , Animais , Linhagem Celular Tumoral , Proliferação de Células/fisiologia , DNA (Citosina-5-)-Metiltransferases/genética , Metilação de DNA , Feminino , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Pessoa de Meia-Idade , Regiões Promotoras Genéticas , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Ubiquitina-Proteína Ligases/genética
16.
Front Public Health ; 9: 651811, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33959581

RESUMO

Background: Currently, more and more subfertility couples are opting for combined acupuncture to improve the success rate of in vitro fertilization and embryo transfer (IVF-ET). However, the efficacy and safety of acupuncture in IVF-ET is still highly controversial. Objectives: The purpose of this overview is to summarize evidence of essential outcomes of systematic reviews (SRs) of acupuncture in IVF-ET and evaluate their methodological quality. Methods: We conducted a comprehensive literature search for relevant SRs in eight databases from inception to July 31, 2020, without language restriction. We evaluated the methodological quality of the included SRs by using A Measurement Tool to Assess Systematic Reviews 2 (AMSTAR-2), which was the latest available assessment tool. The Risk of Bias in Systematic Review (ROBIS) tool was used to assess the risk of bias in SRs. We assessed the Grades of Recommendation, Assessment, Development, and Evaluation (GRADE) score to determine the strength of evidence. We excluded the overlapping randomized controlled trials (RCTs) and performed a re-meta-analysis of the primary RCTs. Results: This review included 312 original RCT studies and 65,388 participants. By using AMSTAR-2, we found that the methodological quality of 16 SRs was critically low, because they had more than one critical weakness. Our reviews showed that although the GRADE for quality of evidence profile was suboptimal, acupuncture seemed to be beneficial in increasing the pregnancy rate. Our re-meta-analysis suggested that acupuncture was superior to sham acupuncture in improving the clinical pregnancy rate (CPR) of IVF-ET with substantial heterogeneity (RR = 1.31, 95% CI: 1.13-1.52, p = 0.0004, I 2 = 66%). No statistical difference was observed regarding the outcomes of live birth rate (LBR), ongoing pregnancy rate (OPR), biochemical pregnancy rate (BPR), and miscarriage rate (MR) between two groups. When compared with no adjunctive treatment groups, acupuncture improved CPR (RR = 1. 25, 95% CI: 1.11-1.42, p = 0.0003) and OPR (RR = 1. 38, 95% CI: 1.04-1.83, p = 0.03). Acupuncture was more superior than no adjunctive treatment in reducing MR (OR = 1.42, 95% CI: 1.03-1.95, p = 0.03) and BPR (RR = 1.19, 95% CI: 1.02-1.37, p = 0.02). Conclusions: Although the evidence of acupuncture in IVF-ET is insufficient, acupuncture appears to be beneficial to increase the clinical pregnancy rate in women undergoing IVF-ET. However, there are severe heterogeneity and methodological quality defects, which limit the reliability of results. Further, high-quality primary studies are still needed.


Assuntos
Terapia por Acupuntura , Infertilidade Feminina , Transferência Embrionária , Feminino , Fertilização In Vitro , Humanos , Infertilidade Feminina/terapia , Gravidez , Revisões Sistemáticas como Assunto
17.
J Nanobiotechnology ; 19(1): 132, 2021 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-33971910

RESUMO

Molecular imaging technology enables us to observe the physiological or pathological processes in living tissue at the molecular level to accurately diagnose diseases at an early stage. Optical imaging can be employed to achieve the dynamic monitoring of tissue and pathological processes and has promising applications in biomedicine. The traditional first near-infrared (NIR-I) window (NIR-I, range from 700 to 900 nm) imaging technique has been available for more than two decades and has been extensively utilized in clinical diagnosis, treatment and scientific research. Compared with NIR-I, the second NIR window optical imaging (NIR-II, range from 1000 to 1700 nm) technology has low autofluorescence, a high signal-to-noise ratio, a high tissue penetration depth and a large Stokes shift. Recently, this technology has attracted significant attention and has also become a heavily researched topic in biomedicine. In this study, the optical characteristics of different fluorescence nanoprobes and the latest reports regarding the application of NIR-II nanoprobes in different biological tissues will be described. Furthermore, the existing problems and future application perspectives of NIR-II optical imaging probes will also be discussed.

18.
Aging (Albany NY) ; 13(11): 15032-15043, 2021 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-34031267

RESUMO

BACKGROUND: Human amniotic epithelial cells (hAECs) are seed cells used to treat acute myocardial infarction (AMI), but their mechanism remains unclear. METHODS: We cultured hAECs and extracted exosomes from culture supernatants. Next, we established a stable AMI model in rats and treated them with hAECs, exosomes, or PBS. We assess cardiac function after treatment by echocardiography. Additionally, heart tissues were collected and analyzed by Masson's trichrome staining. We conducted the tube formation and apoptosis assays to explore the potential mechanisms. RESULTS: Cardiac function was improved, and tissue fibrosis was decreased following implantation of hAECs and their exosomes. Echocardiography showed that the EF and FS were lower in the control group than in the hAEC and exosome groups, and that the LVEDD and LVESD were higher in the control group (P<0.05). Masson's trichrome staining showed that the fibrotic area was larger in the control group. Tube formation was more efficient in the hAEC and exosome groups (P<0.0001). Additionally, the apoptosis rates of myocardial cells in the hAEC and exosome groups were significantly decreased (P<0.0001). CONCLUSIONS: hAECs and their exosomes improved the cardiac function of rats after AMI by promoting angiogenesis and reducing the apoptosis of cardiac myocytes.


Assuntos
Âmnio/citologia , Células Epiteliais/metabolismo , Células Epiteliais/transplante , Exossomos/transplante , Coração/fisiopatologia , Infarto do Miocárdio/fisiopatologia , Infarto do Miocárdio/terapia , Animais , Apoptose , Células Cultivadas , Modelos Animais de Doenças , Eletrocardiografia , Exossomos/metabolismo , Exossomos/ultraestrutura , Fibrose , Humanos , Masculino , Infarto do Miocárdio/diagnóstico por imagem , Miócitos Cardíacos , Ratos Sprague-Dawley
19.
Artigo em Inglês | MEDLINE | ID: mdl-33807451

RESUMO

Lab-scale simulated biofilm reactors, including aerated reactors disturbed by short-term aeration interruption (AE-D) and non-aerated reactors disturbed by short-term aeration (AN-D), were established to study the stable-state (SS) formation and recovery after disturbance for nitrogen transformation in terms of dissolved oxygen (DO), removal efficiency (RE) of NH4+-N and NO3--N and activity of key nitrogen-cycle functional genes amoA and nirS (RNA level abundance, per ball). SS formation and recovery of DO were completed in 0.56-7.75 h after transition between aeration (Ae) and aeration stop (As). In terms of pollutant REs, new temporary SS formation required 30.7-52.3 h after Ae and As interruptions, and seven-day Ae/As interruptions required 5.0% to 115.5% longer recovery times compared to one-day interruptions in AE-D and AN-D systems. According to amoA activity, 60.8 h were required in AE-D systems to establish new temporary SS after As interruptions, and RNA amoA copies (copy number/microliter) decreased 88.5%, while 287.2 h were required in AN-D systems, and RNA amoA copies (copy number/microliter) increased 36.4 times. For nirS activity, 75.2-85.8 h were required to establish new SSs after Ae and As interruptions. The results suggested that new temporary SS formation and recovery in terms of DO, pollutant REs and amoA and nirS gene activities could be modelled by logistic functions. It is concluded that temporary SS formation and recovery after Ae and As interruptions occurred at asynchronous rates in terms of DO, pollutant REs and amoA and nirS gene activities. Because of DO fluctuations, the quantitative relationship between gene activity and pollutant RE remains a challenge.


Assuntos
Desnitrificação , Nitrogênio , Biofilmes , Análise da Demanda Biológica de Oxigênio , Reatores Biológicos , Nitrogênio/análise , Oxigênio , Eliminação de Resíduos Líquidos
20.
Cell Biosci ; 11(1): 74, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33858512

RESUMO

BACKGROUND: As one of the most common malignancy, lung adenocarcinoma (LUAD) is characterized by low 5-year survival rate. This research aimed to investigate the effects of ribonucleotide reductase regulatory subunit M2 (RRM2) on malignant biological behaviors and activation of cGAS/STING pathway. We also explored the synergistic sensitization mechanisms of RRM2 and radiotherapy. METHODS: Bioinformatic tools were used to evaluate the clinical significance of RRM2 in LUAD patients. The roles of RRM2 in malignant phenotype and DNA damage in LUAD cells were investigated with cell proliferation, colony formation, immunofluorescence, modified Boyden chamber and comet assays. The mouse models were used to evaluate the biological significance of RRM2 in vivo. Cytotoxic T cell infiltration was evaluated via flow cytometric analysis and immunohistochemistry staining in C57BL/6 mice. We also explored the synergistic effects of RRM2 silencing and radiation on LUAD cells with apoptosis assay and immunoblotting in vitro. RESULTS: Bioinformatic analysis revealed that RRM2 had diagnostic values for LUAD patients. Higher levels of RRM2 predicted worse prognosis. RRM2 silencing inhibited LUAD cell proliferation, invasion and migration. RRM2 knockdown induced S phase arrest and DNA damage. RRM2 silencing induced cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes (STING) pathway, and the downstream targets were regulated in a STING-dependent manner. Knockdown of RRM2 suppressed tumor growth in the xenograft tumor models. RRM2 deficiency increased CD8 + T cells in the tumor tissues and spleens. Furthermore, RRM2 silencing had synergistic effects with radiation on inhibiting cell proliferation and promoting apoptosis. Meanwhile, this combination promoted the activation of cGAS/STING signaling pathway synergistically, and simultaneously increased expression of IFNß, CCL5 and CXCL10. CONCLUSION: Our results demonstrated that RRM2 silencing had anti-tumor values and activated the cGAS/STING signaling pathway. RRM2 silencing increased CD8 + T cells infiltration. RRM2 silencing cooperated with radiation to inhibit LUAD cell proliferation, promote apoptosis and enhance the activation of cGAS/STING signaling pathway. RRM2 could be a promising target for tumor regression through cancer immunotherapy in LUAD.

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