Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Mais filtros

Base de dados
Intervalo de ano de publicação
Genome Announc ; 2(5)2014 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-25342677


The complex microbiota of pit mud of solid-state fermentation reactors used for the production of Chinese liquor is responsible for producing one of the oldest distillates in the world. We apply a deep-sequencing approach to characterize the microbiota from pits that have been in use for up to 440 years.

Dev Biol ; 248(2): 281-93, 2002 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-12167404


Teeth develop as epithelial appendages, and their morphogenesis is regulated by epithelial-mesenchymal interactions and conserved signaling pathways common to many developmental processes. A key event during tooth morphogenesis is the transition from bud to cap stage when the epithelial bud is divided into specific compartments distinguished by morphology as well as gene expression patterns. The enamel knot, a signaling center, forms and regulates the shape and size of the tooth. Mesenchymal signals are necessary for epithelial patterning and for the formation and maintenance of the epithelial compartments. We studied the expression of Notch pathway molecules during the bud-to-cap stage transition of the developing mouse tooth. Lunatic fringe expression was restricted to the epithelium, where it formed a boundary flanking the enamel knot. The Lunatic fringe expression domains overlapped only partly with the expression of Notch1 and Notch2, which were coexpressed with Hes1. We examined the regulation of Lunatic fringe and Hes1 in cultured explants of dental epithelium. The expression of Lunatic fringe and Hes1 depended on mesenchymal signals and both were positively regulated by FGF-10. BMP-4 antagonized the stimulatory effect of FGF-10 on Lunatic fringe expression but had a synergistic effect with FGF-10 on Hes1 expression. Recombinant Lunatic fringe protein induced Hes1 expression in the dental epithelium, suggesting that Lunatic fringe can act also extracellularly. Lunatic fringe mutant mice did not reveal tooth abnormalities, and no changes were observed in the expression patterns of other Fringe genes. We conclude that Lunatic fringe may play a role in boundary formation of the enamel knot and that Notch-signaling in the dental epithelium is regulated by mesenchymal FGFs and BMP.

Proteínas Morfogenéticas Ósseas/farmacologia , Epitélio/embriologia , Fatores de Crescimento de Fibroblastos/farmacologia , Glicosiltransferases/metabolismo , Proteínas de Membrana/metabolismo , Transdução de Sinais , Dente/embriologia , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos , Proteína Morfogenética Óssea 4 , Proteínas de Ligação a DNA/metabolismo , Epitélio/metabolismo , Fator 10 de Crescimento de Fibroblastos , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Glicosiltransferases/genética , Proteínas de Homeodomínio/metabolismo , Hibridização In Situ , Ligantes , Proteínas de Membrana/genética , Mesoderma/metabolismo , Camundongos , Morfogênese , Mutação , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Receptores Notch , Proteínas Repressoras/metabolismo , Dente/metabolismo , Fatores de Transcrição HES-1