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1.
ACS Nano ; 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36054880

RESUMO

The quest for realizing and manipulating ever smaller man-made movable structures and dynamical machines has spurred tremendous endeavors, led to important discoveries, and inspired researchers to venture to previously unexplored grounds. Scientific feats and technological milestones of miniaturization of mechanical structures have been widely accomplished by advances in machining and sculpturing ever shrinking features out of bulk materials such as silicon. With the flourishing multidisciplinary field of low-dimensional nanomaterials, including one-dimensional (1D) nanowires/nanotubes and two-dimensional (2D) atomic layers such as graphene/phosphorene, growing interests and sustained effort have been devoted to creating mechanical devices toward the ultimate limit of miniaturization─genuinely down to the molecular or even atomic scale. These ultrasmall movable structures, particularly nanomechanical resonators that exploit the vibratory motion in these 1D and 2D nano-to-atomic-scale structures, offer exceptional device-level attributes, such as ultralow mass, ultrawide frequency tuning range, broad dynamic range, and ultralow power consumption, thus holding strong promises for both fundamental studies and engineering applications. In this Review, we offer a comprehensive overview and summary of this vibrant field, present the state-of-the-art devices and evaluate their specifications and performance, outline important achievements, and postulate future directions for studying these miniscule yet intriguing molecular-scale machines.

2.
Foods ; 11(17)2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36076889

RESUMO

To characterize the quality of widely consumed pork in China, the chemical compositions and other indexes of five breeds of pork were compared. The results indicated that the moisture content, sensory flavor, and overall acceptability of Pipa pork (PPP) were superior to other breeds. The fat content and essential amino acids (EAA) of Yihao native pork (YNP) were significantly (p < 0.05) higher than in other breeds. The protein content, the total amount of amino acids, and perceptible flavor of Tibetan black pork (TBP) were higher than in other breeds. The protein nutrition profiles of manor black pork (MBP) and TBP were better than in others. The equivalent umami concentration (EUC) value of white pork (WP) was significantly (p < 0.05) higher than in others; however, the health risk of its fatty acid content was higher. There were unique protein and flavor chemicals in YNP, TBP, and PPP, which may be useful for distinguishing their authenticity.

3.
Mol Med ; 28(1): 106, 2022 09 06.
Artigo em Inglês | MEDLINE | ID: mdl-36068514

RESUMO

BACKGROUND: Acute pancreatitis is the sudden inflammation of the pancreas. Severe cases of acute pancreatitis are potentially fatal and have no specific treatment available. Premature trypsinogen activation could initiate acute pancreatitis. However, the mechanism underlying premature trypsinogen activation is not fully understood. METHODS: In this research, a primary pancreatic acinar cell or mouse acute pancreatitis model was constructed. The effect of acid ceramidase (ASAH1), which is responsible for sphingosine production, was investigated in trypsinogen activation in vitro and in vivo. Meanwhile, the proteins regulating ASAH1 or binding to sphingosine were also detected by co-immunoprecipitation followed by mass spectrometry. RESULTS: The results showed that ASAH1 increased in acute pancreatitis. Increased ASAH1 promoted the activation of trypsinogen and cathepsin B. On the contrary, ASAH1 downregulation inhibited trypsinogen and cathepsin B. Meanwhile, ASAH1 regulated the activity of trypsin and cathepsin B through sphingosine. Additionally, E3 ligase Mind bomb homolog 1 (MIB1) decreased in acute pancreatitis resulting in the decreased binding between MIB1 and ASAH1. Exogenous MIB1 diminished the elevation in trypsin activity induced by acute pancreatitis inducer. ASAH1 increased owing to the inhibition of the proteasome degradation by MIB1. In acute pancreatitis, sphingosine was found to bind to pyruvate kinase. Pyruvate kinase activation could reduce trypsinogen activation and mitochondrial reactive oxygen species (ROS) production induced by sphingosine. CONCLUSIONS: In conclusion, during the process of acute pancreatitis, MIB1 downregulation led to ASAH1 upregulation, resulting in pyruvate kinase inhibition, followed by trypsinogen activation.


Assuntos
Pancreatite , Tripsinogênio , Ceramidase Ácida , Doença Aguda , Animais , Catepsina B/metabolismo , Modelos Animais de Doenças , Camundongos , Pancreatite/induzido quimicamente , Pancreatite/metabolismo , Piruvato Quinase , Esfingosina/efeitos adversos , Tripsina/metabolismo , Tripsinogênio/metabolismo
4.
Comput Math Methods Med ; 2022: 1524198, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35979054

RESUMO

Objective: The purpose of this study is to explore and analyze the preventive effect of esketamine on postpartum depression (PPD) after cesarean section. Methods: A total of 138 puerperae who underwent cesarean section in our hospital from February 2020 to January 2022 were selected as the research subjects. The control group was given intravenous injection of 2 ml of normal saline after the fetus was delivered. Meanwhile, the observation group was given intravenous injection of a small dose of esketamine (esketamine 0.5 mg/kg+ 2 ml of normal saline) after the delivery of the fetus. The changes of blood pressure and heart rate, the Edinburgh Postnatal Depression Scale (EPDS) questionnaire scores and the incidence of postpartum depression were compared between the two groups. At the same time, the incidence of postoperative adverse events in the two groups was observed. Results: There was no significant difference in systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) between the two groups at T1-T3 (P > 0.05). Compared with the control group, the SBP, DBP, and HR at T4 and T5 in the observation group were higher (P < 0.05). There was no significant difference in SBP, DBP, and HR at T3, T4, and T5 in the observation group (P > 0.05). Compared with T3, SBP, DBP, and HR were lower in control group T4 and T5, respectively. There was no significant difference in the EPDS scores between the two groups on the 1st day before delivery (P > 0.05). The EPDS scores of the two groups were higher at 3d postpartum and 42d postpartum, respectively, than at 1d before delivery. The EPDS scores of the observation group at 3d and 42d after delivery were lower than those in the control group (P < 0.05). Compared with the control group, the incidence of postpartum depression was higher in the observation group at 3 days postpartum and 1 month postpartum, respectively (P < 0.05). There was no significant difference in the incidence of postpartum adverse reactions between the two groups (P > 0.05). Conclusion: The application of esketamine after cesarean section can effectively reduce depression-related scores and the risk of postpartum depression without increasing adverse reactions and has high safety.


Assuntos
Cesárea , Depressão Pós-Parto , Ketamina , Pressão Sanguínea/efeitos dos fármacos , Depressão Pós-Parto/prevenção & controle , Feminino , Frequência Cardíaca/efeitos dos fármacos , Humanos , Ketamina/administração & dosagem , Ketamina/efeitos adversos , Ketamina/farmacologia , Período Pós-Parto , Gravidez
5.
Nano Lett ; 22(14): 5780-5787, 2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35792575

RESUMO

Resonant nanoelectromechanical systems (NEMS) enabled by two-dimensional (2D) semiconductors have been actively explored and engineered for making ultrascaled transducers toward applications in ultralow-power signal processing, communication, and sensing. Although the transduction of miniscule resonant motions has been achieved by low-noise optical or electronic techniques, direct probing of strain in vibrating 2D semiconductor membranes and the interplay between the spectroscopic and mechanical properties are still largely unexplored. Here, we experimentally demonstrate dynamical phonon softening in atomically thin molybdenum disulfide (MoS2) NEMS resonators by directly coupling Raman spectroscopy with optical interferometry resonance motion detection. In single-layer, bilayer, and trilayer (1L to 3L) MoS2 circular membrane NEMS resonators, we show that high-amplitude nonlinear resonances can enhance the Raman signal amplitude, as well as introduce Raman modes softening up to 0.8 cm-1. These results shall pave the way for engineering the coupling and control between collective mechanical vibrations and Raman modes of the constituent crystals in 2D transducers.

6.
Foods ; 11(13)2022 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-35804641

RESUMO

To evaluate the storage stability and flavor changes of marinated pork treated with chili and pepper essential oils, the contents of total sulfhydryl, malondialdehyde, total volatile base nitrogen (TVBN), Ca2+ATPase activity, and total viable counts of marinated pork were determined. Further, the non-volatile (umami, numb, and spicy) and volatile flavor compounds of marinated pork were analyzed. Based on the results, the chili and pepper essential oils had limited effects on the storage stability of marinated pork. However, these essential oils could inhibit the oxidation of lipids and proteins and reduce the number of microorganisms and TVBN in marinated pork within 6 days. The non-volatile flavors of the marinated pork decreased as the refrigeration time increased. It was concluded that the decomposition of umami-enhancing nucleotides (GMP, IMP, XMP), the number of flavor substances (hydroxyl-α-sanshool, hydroxyl-ß-sanshool), and spicy (capsaicin) tasting compounds caused the decrease in non-volatile flavors.

7.
Adv Mater ; 34(33): e2205462, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35759925

RESUMO

Lung metastasis is challenging in patients with triple-negative breast cancer (TNBC). Surgery is always not available due to the dissemination of metastatic foci and most drugs are powerless because of poor retention at metastatic sites. TNBC cells generate an inflamed microenvironment and overexpress adhesive molecules to promote invasion and colonization. Herein, "walking dead" TNBC cells are developed through conjugating anti-PD-1 (programmed death protein 1 inhibitor) and doxorubicin (DOX)-loaded liposomes onto cell corpses for temporal chemo-immunotherapy against lung metastasis. The walking dead TNBC cells maintain plenary tumor antigens to conduct vaccination effects. Anti-PD-1 antibodies are conjugated to cell corpses via reduction-activated linker, and DOX-loaded liposomes are attached by maleimide-thiol coupling. This anchor strategy enables rapid release of anti-PD-1 upon reduction conditions while long-lasting release of DOX at inflamed metastatic sites. The walking dead TNBC cells improve pulmonary accumulation and local retention of drugs, reprogram the lung microenvironment through damage-associated molecular patterns (DAMPs) and PD-1 blockade, and prolong overall survival of lung metastatic 4T1 and EMT6-bearing mice. Taking advantage of the walking dead TNBC cells for pulmonary preferred delivery of chemotherapeutics and checkpoint inhibitors, this study suggests an alternative treatment option of chemo-immunotherapy to augment the efficacy against lung metastasis.


Assuntos
Neoplasias Pulmonares , Neoplasias de Mama Triplo Negativas , Animais , Cadáver , Linhagem Celular Tumoral , Humanos , Lipossomos/uso terapêutico , Neoplasias Pulmonares/tratamento farmacológico , Camundongos , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Neoplasias de Mama Triplo Negativas/patologia , Microambiente Tumoral
8.
Bioinformatics ; 2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35771644

RESUMO

SUMMARY: Characterizing biomarkers based on microbiome profiles has great potential for translational medicine and precision medicine. Here, we present microbiomeMarker, an R/Bioconductor package implementing commonly used normalization and differential analysis methods, and three supervised learning models to identify microbiome markers. microbiomeMarker also allows comparison of different methods of differential analysis and confounder analysis. It uses standardized input and output formats, which renders it highly scalable and extensible, and allows it to seamlessly interface with other microbiome packages and tools. In addition, the package provides a set of functions to visualize and interpret the identified microbiome markers. AVAILABILITY AND IMPLEMENTATION: microbiomeMarker is freely available from Bioconductor (https://www.bioconductor.org/packages/microbiomeMarker). Source code is available and maintained at GitHub (https://github.com/yiluheihei/microbiomeMarker). SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

9.
FEBS Lett ; 596(17): 2215-2230, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35615915

RESUMO

Seed storage proteins (SSPs) accumulated within plant seeds constitute the major protein nutrition sources for human and livestock. SSPs are synthesized on the endoplasmic reticulum and are then deposited in plant-specific protein bodies, including endoplasmic reticulum-derived protein bodies and protein storage vacuoles. Plant seeds have evolved a distinct endomembrane system to accomplish SSP transport. There are two distinct types of trafficking pathways contributing to SSP delivery to protein storage vacuoles: one is Golgi-dependent and the other is Golgi-independent. In recent years, molecular, genetic, and biochemical studies have shed light on the complex network controlling SSP trafficking, to which both evolutionarily conserved molecular machineries and plant-unique regulators contribute. In this review, we discuss current knowledge of protein body biogenesis and endomembrane-mediated SSP transport, focusing on endoplasmic reticulum export and post-Golgi traffic. This knowledge supports a dominant role for the Golgi-dependent pathways in SSP transport in Arabidopsis and rice. In addition, we describe cutting-edge strategies for dissecting the endomembrane trafficking system in plant seeds to advance the field.


Assuntos
Arabidopsis , Complexo de Golgi , Proteínas de Plantas , Transporte Proteico , Arabidopsis/genética , Arabidopsis/metabolismo , Complexo de Golgi/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas/metabolismo , Proteínas de Armazenamento de Sementes/metabolismo , Sementes/genética , Vacúolos/metabolismo
10.
Biomed Res Int ; 2022: 9506227, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35578723

RESUMO

Certain plant growth-promoting bacteria (PGPB) reduce salt stress damage in plants. Bacillus subtilis HG-15 is a halotolerant bacterium (able to withstand NaCl concentrations as high as 30%) isolated from the wheat rhizoplane in the Yellow River delta. A qualitative and quantitative investigation of the plant growth-promoting characteristics of this strain confirmed nitrogen fixation, potassium dissolution, ammonia, plant hormone, ACC deaminase, and proline production abilities. B. subtilis HG-15 colonization of wheat roots, stems, and leaves was examined via scanning electron microscopy, rep-PCR, and double antibiotic screening. After inoculation with the B. subtilis HG-15 strain, the pH (1.08-2.69%), electrical conductivity (3.17-11.48%), and Na+ (12.98-15.55%) concentrations of rhizosphere soil significantly decreased (p < 0.05). Under no-salt stress (0.15% NaCl), low-salt stress (0.25% NaCl), and high-salt stress (0.35% NaCl) conditions, this strain also significantly increased (p < 0.05) the dry weight (17.76%, 24.46%, and 9.31%), fresh weight (12.80%, 20.48%, and 7.43%), plant height (7.79%, 5.86%, and 13.13%), and root length (10.28%, 17.87%, and 48.95%). Our results indicated that B. subtilis HG-15 can effectively improve the growth of wheat and elicit induced systemic tolerance in these plants, thus showing its potential as a microbial inoculant that can protect wheat under salt stress conditions.


Assuntos
Tolerância ao Sal , Triticum , Bacillus subtilis/genética , Raízes de Plantas/microbiologia , Salinidade , Tolerância ao Sal/genética , Cloreto de Sódio/farmacologia , Triticum/genética
11.
Lab Chip ; 22(13): 2476-2488, 2022 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-35521650

RESUMO

Small vesicles (sEVs) are closely related to many diseases as they carry various bio-markers. Efficient separation of sEVs from complex biological samples is essential and prerequisite for the following treatment and further disease diagnosis. Here we propose a label-free and biocompatible on-chip magnetic separation system for efficient extraction of sEVs from cell culture supernatant. Through an on-chip ultra-high gradient magnetic field module, a magnetic field gradient close to 100 000 T m-1 is generated inside the separation microchannel. By using fluorescent particles of 200 nm and 1000 nm to simulate sEVs and other bioparticles in a complex sample, the system design and the experimental parameters are optimized. Flow cytometry and a proposed fluorescence intensity analysis method both verify that the recovery rate and purity of 200 nm particles can reach 84.91% and 98.02%, respectively. Then, a biocompatible ferrofluid is utilized in the separation system to separate sEVs from the cell culture supernatant. The results tested by nanoparticle tracking analysis show that the recovery rate and purity of sEVs are 85.80% and 80.45%, superiorly exceeding the performance that the ultracentrifugation method can provide.


Assuntos
Vesículas Extracelulares , Técnicas de Cultura de Células , Fenômenos Magnéticos
12.
Nat Cancer ; 3(5): 565-580, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35624341

RESUMO

Among the greatest hurdles in clinical management of prostate cancer (PCa) are the progression to lethal castration-resistant prostate cancer (CRPC) and the lack of suitable targeted therapies for advanced disease. Here we identify Gremlin1 as a ligand for fibroblast growth factor receptor 1 (FGFR1), which promotes lineage plasticity and drives castration resistance. Importantly, we generate a specific anti-Gremlin1 therapeutic antibody and demonstrate synergistic effect with androgen deprivation therapy (ADT) in CRPC. GREM1 transcription is suppressed by androgen receptor (AR) and released following ADT. We show that Gremlin1 binds to FGFR1 and activates downstream MAPK signaling. Gremlin1 interacts with FGFR1 differently to its canonical ligand FGF1, as revealed through protein structure docking and mutagenesis experiments. Altogether, our data indicate Gremlin1 as a promising candidate therapeutic target for CRPC.


Assuntos
Neoplasias de Próstata Resistentes à Castração , Antagonistas de Androgênios/farmacologia , Castração , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Ligantes , Masculino , Neoplasias de Próstata Resistentes à Castração/tratamento farmacológico , Neoplasias de Próstata Resistentes à Castração/genética , Neoplasias de Próstata Resistentes à Castração/metabolismo , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/genética , Transdução de Sinais
13.
J Xray Sci Technol ; 30(4): 709-724, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35404300

RESUMO

The objective of this study is to apply an improved Faster-RCNN model in order to solve the problems of low detection accuracy and slow detection speed in spark plug defect detection. In detail, an attention module based symmetrical convolutional network (ASCN) is designed as the backbone to extract multi-scale features. Then, a multi-scale region generation network (MRPN), in which InceptionV2 is used to achieve sliding windows of different scales instead of a single sliding window, is proposed and tested. Additionally, a dataset of X-ray spark plug images is established, which contains 1,402 images. These images are divided into two subsets with a ratio of 4:1 for training and testing the improved Faster-RCNN model, respectively. The proposed model is transferred and learned on the pre-training model of MS COCO dataset. In the test experiments, the proposed method achieves an average accuracy of 89% and a recall of 97%. Compared with other Faster-RCNN models, YOLOv3, SSD and RetinaNet, our proposed new method improves the average accuracy by more than 6% and the recall by more than 2%. Furthermore, the new method can detect at 20fps when the input image size is 1024×1024×3 and can also be used for real-time automatic detection of spark plug defects.


Assuntos
Algoritmos , Redes Neurais de Computação
14.
Front Microbiol ; 13: 863341, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35464941

RESUMO

Meloidogyne incognita is one of the most destructive soil pests, causing serious economic losses in tomato production. Here, in vitro experiments demonstrated that the Bacillus licheniformis strain JF-22 has the potential to prevent M. incognita infection. A pot experiment confirmed that B. licheniformis strain JF-22 isolated from the tomato rhizosphere soil and planted in the tomato root-knot nematode disease area effectively prevented and controlled M. incognita, reducing its negative effect on tomato growth. Additionally, the composition of volatile substances secreted by B. licheniformis strain JF-22 was analyzed using solid-phase microextraction and gas chromatography-mass spectrometry. We detected acetoin, 2,3-Butanediol, [R-(R*,R*) ]-, and hexamethyl cyclotrisiloxane as the main components among these volatiles. Using MiSeq sequencing technology and bioinformatics, we analyzed the influence of B. licheniformis strain JF-22 on the microbial community of the tomato rhizosphere. B. licheniformis strain JF-22 changed the composition of the microbial community; particularly, it significantly reduced the diversity of the fungal community. Furthermore, using the FUNGuild and PICRUSt databases, we predicted the effect of JF-22 on microbial community function. In conclusion, B. licheniformis strain JF-22 may be considered as a potential biocontrol agent against M. incognita.

16.
Anal Methods ; 14(14): 1439-1444, 2022 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-35322264

RESUMO

Gold nanoclusters (AuNCs) have large Stokes shifts and long fluorescence life, which make them have high application value in bioanalytical applications. However, the quantum yield (QY) of AuNCs was lower, which hinders their wide application. Herein, a facile, novel and one-pot approach was developed to synthesize AuNCs@zinc-glutamate metal organic frameworks (AuNCs@ZnGlu-MOFs (product)). The product was easily prepared via mixing the glutathione (GSH) protected AuNCs with ZnGlu-MOF precursors. Compared with GSH-AuNCs, the ultrahigh QY (33.18%) of the AuNCs@ZnGlu-MOF is nearly 6 times higher. In addition, the product possesses better water stability and longer luminescence life (9.86 µs) due to the protective and confinement effects of the ZnGlu-MOF. Particularly, the product has a unique spatial structure, which can effectively prevent the interaction between large-size biothiols (such as cysteine and homocysteine) and the product, thus significantly improving the selectivity of it. Based on the excellent optical advantages of this product, it was capable of being applied as a selectable and sensitive fluorescence probe to detect H2O2 and H2O2-related analytes. This method has also been further employed in the precise H2O2-monitoring in serum, which is promising in the application of clinical bioassay.


Assuntos
Nanopartículas Metálicas , Estruturas Metalorgânicas , Ácido Glutâmico , Glutationa/química , Ouro/química , Peróxido de Hidrogênio , Nanopartículas Metálicas/química , Estruturas Metalorgânicas/química , Zinco
17.
IEEE J Biomed Health Inform ; 26(7): 3251-3260, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35239495

RESUMO

Generative adversarial networks (GAN) have shown great potential for image quality improvement in low-dose CT (LDCT). In general, the shallow features of generator include more shallow visual information such as edges and texture, while the deep features of generator contain more deep semantic information such as organization structure. To improve the network's ability to categorically deal with different kinds of information, this paper proposes a new type of GAN with dual-encoder- single-decoder structure. In the structure of the generator, firstly, a pyramid non-local attention module in the main encoder channel is designed to improve the feature extraction effectiveness by enhancing the features with self-similarity; Secondly, another encoder with shallow feature processing module and deep feature processing module is proposed to improve the encoding capabilities of the generator; Finally, the final denoised CT image is generated by fusing main encoder's features, shallow visual features, and deep semantic features. The quality of the generated images is improved due to the use of feature complementation in the generator. In order to improve the adversarial training ability of discriminator, a hierarchical-split ResNet structure is proposed, which improves the feature's richness and reduces the feature's redundancy in discriminator. The experimental results show that compared with the traditional single-encoder- single-decoder based GAN, the proposed method performs better in both image quality and medical diagnostic acceptability. Code is available in https://github.com/hanzefang/DESDGAN.


Assuntos
Processamento de Imagem Assistida por Computador , Semântica , Humanos , Processamento de Imagem Assistida por Computador/métodos , Tomografia Computadorizada por Raios X/métodos
18.
Abdom Radiol (NY) ; 47(6): 2046-2056, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35306577

RESUMO

PURPOSE: Determine whether liver attenuation measured on dual-energy CT (DECT) virtual non-contrast examinations predicts the presence of fatty liver. METHODS: Single-institution retrospective review from 2016 to 2020 found patients with DECT and proton density fat fraction MRI (MRI PDFF) within 30 days. MRI PDFF was the reference standard for determining hepatic steatosis. Attenuation measurements from VNC and mixed 120 kVp-like images were compared to MRI PDFF in the right and left lobes. Performance of VNC was compared to measurement of the liver-spleen attenuation difference (LSAD). RESULTS: 128 patients were included (69 men, 59 women) with mean age 51.6 years (range 14-98 years). > 90% of patients received CT and MRI in the emergency department or as inpatients. Median interval between DECT and MRI PDFF was 2 days (range 0-28 days). Prevalence of fatty liver using the reference standard (MRI PDFF > 6%) was 24%. Pearson correlation coefficient between VNC and MRI- DFF was -0.64 (right) and -0.68 (left, both p < 0.0001). For LSAD, correlation was - 0.43 in both lobes (p < 0.0001). Considering MRI PDFF > 6% as diagnostic of steatosis, area under the receiver operator characteristic curve (AUC) was 0.834 and 0.872 in the right and left hepatic lobes, with an optimal threshold of 54.8 HU (right) and 52.5 HU (left), yielding sensitivity/specificity of 57%/93.9% (right) and 67.9%/90% (left). For LSAD, AUC was 0.808 (right) and 0.767 (left) with optimal sensitivity/specificity of 93.3%/57.1% (right) and 78.6%/68% (left). CONCLUSION: Attenuation measured at VNC CT was moderately correlated with liver fat content and had > 90% specificity for diagnosis of fatty liver.


Assuntos
Fígado Gorduroso , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Fígado Gorduroso/diagnóstico por imagem , Feminino , Humanos , Fígado/diagnóstico por imagem , Imageamento por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos , Adulto Jovem
19.
Funct Integr Genomics ; 22(3): 423-428, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35267109

RESUMO

Spinach RNA-mimicking GFP (S-RMG) has been successfully used to monitor cellular RNAs including microRNAs in bacterium, yeast, and human cells. However, S-RMG has not been established in plants. In this study, we found that like bacterial, yeast, and human cellular tRNAs, plant tRNAs such as tRNALys can protect and/or stabilize the Spinach RNA aptamer interaction with the fluorophore DFHBI enabling detectable levels of green fluorescence to be emitted. The tRNALys-Spinach-tRNALys, once delivered into "chloroplast-free" onion epidermal cells can emit strong green fluorescence in the presence of DFHBI. Our results demonstrate for the first time that Spinach-based RNA visualization has the potential for in vivo monitoring of RNAs in plant cells.


Assuntos
RNA , Spinacia oleracea , Humanos , Células Vegetais , Plantas/genética , RNA de Plantas/genética , RNA de Transferência , RNA de Transferência de Lisina , Saccharomyces cerevisiae/genética , Spinacia oleracea/genética
20.
Cells ; 11(5)2022 02 26.
Artigo em Inglês | MEDLINE | ID: mdl-35269443

RESUMO

The phosphoinositide-3-kinase (PI3K)/AKT pathway regulates cell survival and is over-activated in most human cancers, including ovarian cancer. Following growth factor stimulation, AKT1 is activated by phosphorylation at T308 and S473. Disruption of the AKT1 signaling pathway is sufficient to inhibit the epithelial-mesenchymal transition in epithelial ovarian cancer (EOC) cells. In metastatic disease, adherent EOC cells transition to a dormant spheroid state, characterized previously by low S473 phosphorylation in AKT1. We confirmed this finding and observed that T308 phosphorylation was yet further reduced in EOC spheroids and that the transition from adherent to spheroid growth is accompanied by significantly increased levels of let-7 miRNAs. We then used mechanistic studies to investigate the impact of let-7 miRNAs on AKT1 phosphorylation status and activity in cells. In growth factor-stimulated HEK 293T cells supplemented with let-7a, we found increased phosphorylation of AKT1 at T308, decreased phosphorylation at S473, and enhanced downstream AKT1 substrate GSK-3ß phosphorylation. Let-7b and let-7g also deregulated AKT signaling by rendering AKT1 insensitive to growth factor simulation. We uncovered let-7a-dependent deregulation of PI3K pathway components, including PI3KC2A, PDK1, and RICTOR, that govern AKT1 phosphorylation and activity. Together, our data show a new role for miRNAs in regulating AKT signaling.


Assuntos
MicroRNAs , Neoplasias Ovarianas , Carcinoma Epitelial do Ovário , Glicogênio Sintase Quinase 3 beta/metabolismo , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo
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