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1.
Adv Mater ; : e2211555, 2023 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-37149287

RESUMO

Manganese-based aqueous batteries utilizing Mn2+ /MnO2 redox reactions are promising choices for grid-scale energy storage due to their high theoretical specific capacity, high power capability, low-cost, and intrinsic safety with water-based electrolytes. However, the application of such systems is hindered by the insulating nature of deposited MnO2 , resulting in low normalized areal loading (0.005∼0.05 mAh cm-2 ) during charge/discharge cycle. In this work, we investigated the electrochemical performance of various MnO2 polymorphs in Mn2+ /MnO2 redox reactions and determined ɛ-MnO2 with low conductivity to be the primary electrochemically deposited phase in normal acidic aqueous electrolyte. We found that increasing the temperature can change the deposited phase from ɛ-MnO2 with low conductivity to γ-MnO2 with two orders of magnitude increase in conductivity. We demonstrated that the highly conductive γ-MnO2 could be effectively exploited for ultrahigh areal loading electrode, and a normalized areal loading of 33 mAh cm-2 was achieved. At a mild temperature of 50 °C, cells were cycled with an ultrahigh areal loading of 20 mAh cm-2 (1-2 orders of magnitude higher than previous studies) for over 200 cycles with only 13% capacity loss. This article is protected by copyright. All rights reserved.

2.
Artigo em Inglês | MEDLINE | ID: mdl-37159310

RESUMO

Trajectory forecasting for traffic participants (e.g., vehicles) is critical for autonomous platforms to make safe plans. Currently, most trajectory forecasting methods assume that object trajectories have been extracted and directly develop trajectory predictors based on the ground truth trajectories. However, this assumption does not hold in practical situations. Trajectories obtained from object detection and tracking are inevitably noisy, which could cause serious forecasting errors to predictors built on ground truth trajectories. In this paper, we propose to predict trajectories directly based on detection results without relying on explicitly formed trajectories. Different from traditional methods which encode the motion cues of an agent based on its clearly defined trajectory, we extract the motion information only based on the affinity cues among detection results, in which an affinity-aware state update mechanism is designed to manage the state information. In addition, considering that there could be multiple plausible matching candidates, we aggregate the states of them. These designs take the uncertainty of association into account which relax the undesirable effect of noisy trajectory obtained from data association and improve the robustness of the predictor. Extensive experiments validate the effectiveness of our method and its generalization ability to different detectors or forecasting schemes.

3.
J Environ Manage ; 342: 118065, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-37196611

RESUMO

Excessive phosphorus (P) along with drained water from farmland in the arid and semiarid watersheds when entering into water bodies brings about serious environmental problems in the aquatic ecosystem. It is critical to explore variations in watershed P balance and the relationship between anthropogenic P input and riverine total phosphorus (TP) export in a typical irrigation watersheds. In this study, long-term anthropogenic P variations in Ulansuhai Nur watershed (UNW), a typical irrigation watershed in Yellow River basin, was investigated using a quantitative Net Anthropogenic Phosphorus Input (NAPI) budget model. The results showed that annual NAPI exhibited a significant upward trend with a multi-year average of 2541.6 kg P km-2 yr-1 in the UNW. Hotspots for watershed NAPI were discovered in Linhe and Hangjin Houqi counties. Chemical P fertilizers and livestock breeding were two dominated sources of NAPI. Annual riverine TP export showed a significantly declined trend with a net decrease of 80.6%. The export ratio of watershed NAPI was 0.6%, lower than those reported for other watersheds worldwide. There was a significant positive linear correlation between NAPI and riverine TP export from 2005 to 2009. However, after 2009, riverine TP export exhibited a decreased trend with increasing watershed NAPI, which was attributed to environmental treatment measures. By reconstructing riverine TP export without the impact of pollution treatment measures, annual average reduction amount of riverine TP export from 2009 to 2019 was estimated to be 237.2 ton, 47.2% and 52.8% of which were attributed to the point and nonpoint sources measures. This study not only widens the application scope of NAPI budget method, but also provides useful information of nutrient management and control in the arid and semiarid irrigation watershed.

4.
Nucleic Acids Res ; 51(8): 3793-3805, 2023 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-37014011

RESUMO

Maternal mitochondria are the sole source of mtDNA for every cell of the offspring. Heteroplasmic mtDNA mutations inherited from the oocyte are a common cause of metabolic diseases and associated with late-onset diseases. However, the origin and dynamics of mtDNA heteroplasmy remain unclear. We used our individual Mitochondrial Genome sequencing (iMiGseq) technology to study mtDNA heterogeneity, quantitate single nucleotide variants (SNVs) and large structural variants (SVs), track heteroplasmy dynamics, and analyze genetic linkage between variants at the individual mtDNA molecule level in single oocytes and human blastoids. Our study presented the first single-mtDNA analysis of the comprehensive heteroplasmy landscape in single human oocytes. Unappreciated levels of rare heteroplasmic variants well below the detection limit of conventional methods were identified in healthy human oocytes, of which many are reported to be deleterious and associated with mitochondrial disease and cancer. Quantitative genetic linkage analysis revealed dramatic shifts of variant frequency and clonal expansions of large SVs during oogenesis in single-donor oocytes. iMiGseq of a single human blastoid suggested stable heteroplasmy levels during early lineage differentiation of naïve pluripotent stem cells. Therefore, our data provided new insights of mtDNA genetics and laid a foundation for understanding mtDNA heteroplasmy at early stages of life.


Assuntos
DNA Mitocondrial , Células-Tronco Pluripotentes , Humanos , DNA Mitocondrial/genética , DNA Mitocondrial/metabolismo , Haplótipos , Heteroplasmia , Mitocôndrias/genética , Mitocôndrias/metabolismo , Oócitos/metabolismo , Células-Tronco Pluripotentes/metabolismo
5.
Theranostics ; 13(5): 1632-1648, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37056566

RESUMO

Background: Singlet oxygen (1O2) has received considerable research attention in photodynamic therapy (PDT) due to its cytotoxic solid features. However, the inherent hypoxic state of the tumor microenvironment (TME) leads to the meager 1O2 quantum yield of inorganic PDT reagents, and their application in vivo remains elusive. Methods: We developed a novel strategy to fabricate active photosynthetic bacteria/photosensitizer/photothermal agent hybrids for photosynthetic tumor oxygenation and PDT and PTT tumor therapy under different laser irradiation sources. Photosynthetic bacteria combined with Ce6 photosensitizer and Au NPs photothermal agent, the obtained Bac@Au-Ce6 effectively targets tumor tissues and further enhances the tumor accumulation of Au-Ce6. Results: The results showed that the Au-Ce6-loaded engineered bacteria (Bac@Au-Ce6) maintained the photosynthetic properties of Syne. After i.v. injection, Bac@Au-Ce6 efficiently aggregates at tumor sites due to the tumor-targeting ability of active Syne. With 660 nm laser irradiation at the tumor site, the photoautotrophic Syne undergoes sustained photosynthetic O2 release and immediately activates O2 to 1O2 via a loaded photosensitizer. PTT was subsequently imparted by 808 laser irradiations to enhance tumor killing further. Conclusions: This work provides a new platform for engineering bacteria-mediated photosynthesis to promote PDT combined with PTT multi-faceted anti-tumor.


Assuntos
Neoplasias , Fotoquimioterapia , Humanos , Fármacos Fotossensibilizantes/uso terapêutico , Fotoquimioterapia/métodos , Microambiente Tumoral , Luz , Neoplasias/tratamento farmacológico , Hipóxia/tratamento farmacológico , Linhagem Celular Tumoral
6.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 37(4): 391-397, 2023 Apr 15.
Artigo em Chinês | MEDLINE | ID: mdl-37070303

RESUMO

Objective: To evaluate the effectiveness of tendon insertion medialized repair in treatment of large-to-massive rotator cuff tears (L/MRCT). Methods: The clinical and imaging data of 46 L/MRCT patients who underwent arthroscopic insertion medialized repair between October 2015 and June 2019 were retrospectively analyzed. There were 26 males and 20 females with an average age of 57.7 years (range, 40-75 years). There were 20 cases of large rotator cuff tears and 26 cases of massive rotator cuff tears. Preoperative imaging evaluation included fatty infiltration (Goutallier grade), tendon retraction (modified Patte grade), supraspinatus tangent sign, acromiohumeral distance (AHD), and postoperative medializaiton length and tendon integrity. The clinical outcome was evaluated by visual analogue scale (VAS) score, American Society for Shoulder and Elbow Surgery (ASES) score, shoulder range of motion (including anteflexion and elevation, lateral external, and internal rotation) and anteflexion and elevation muscle strength before and after operation. The patients were divided into two groups (the intact tendon group and the re-teared group) according to the integrity of the tendon after operation. According to the medializaiton length, the patients were divided into group A (medialization length ≤10 mm) and group B (medialization length >10 mm). The clinical function and imaging indexes of the patients were compared. Results: All patients were followed up 24-56 months, with an average of 31.8 months. At 1 year after operation, MRI showed that the medializaiton length of supraspinatus tendon was 5-15 mm, with an average of 10.26 mm, 33 cases in group A and 13 cases in group B. Eleven cases (23.91%) had re-teared, including 5 cases (45.45%) of Sugaya type Ⅳ and 6 cases (54.55%) of Sugaya type Ⅴ. At last follow-up, the VAS score, ASES score, shoulder anteflexion and elevation range of motion, lateral external rotation range of motion, and anteflexion and elevation muscle strength significantly improved when compared with those before operation ( P<0.05); there was no significant difference in internal rotation range of motion between pre- and post-operation ( P>0.05). The Goutallier grade and modified Patte grade of supraspinatus muscle in the re-teared group were significantly higher than those in the intact tendon group, and the AHD was significantly lower than that in the intact tendon group ( P<0.05). There was no significant difference in other baseline data between the two groups ( P>0.05). Except that the ASES score of the intact tendon group was significantly higher than that of the re-teared group ( P<0.05), there was no significant difference in the other postoperative clinical functional indicators between the two groups ( P>0.05). There was no significant difference in the incidence of re-tear, VAS score, ASES score, range of motion of shoulder joint, and anteflexion and elevation muscle strength between group A and group B ( P>0.05). Conclusion: Tendon insertion medialized repair may be useful in cases with L/MRCT, and shows good postoperative shoulder function. Neither tendon integrity nor medialization length shows apparent correlations with postoperative shoulder function.


Assuntos
Lesões do Manguito Rotador , Articulação do Ombro , Masculino , Feminino , Humanos , Pessoa de Meia-Idade , Lesões do Manguito Rotador/diagnóstico por imagem , Lesões do Manguito Rotador/cirurgia , Estudos Retrospectivos , Resultado do Tratamento , Manguito Rotador/diagnóstico por imagem , Manguito Rotador/cirurgia , Tendões , Ruptura/cirurgia , Articulação do Ombro/cirurgia , Artroscopia/métodos , Amplitude de Movimento Articular
7.
Front Med (Lausanne) ; 10: 982016, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37089596

RESUMO

Objective: To systematically evaluate the clinical efficacy and safety of Shenkang injection (SKI) combined with alprostadil in the treatment of chronic renal failure (CRF). Method: Randomized controlled trials (RCTs) of Shenkang injection combined with alprostadil in CRF treatment were investigated by retrieving a total of 7 databases including CNKI, Wanfang database, VIP, CBM, PubMed, Embase and Cochrane Library, with the search time ranging from 2012 to now. Revman 5.2 software was used for data analysis, and Cochrane bias risk tool was used to evaluate the quality of the included literature. The final results were represented by relative risk (RR), mean difference (MD) and 95% confidence interval (95% CI). Results: A total of 20 RCTs and 1,573 patients were included in this study. Meta-analysis showed that the overall response rate (ORR) of the treatment group was superior to the control group [RR = 0.20, 95% CI (0.16, 0.25), P < 0.00001]. Compared with the control group, the treatment group achieved favorable improvement in terms of the creatinine clearance rate (Ccr) [MD = 9.48, 95% CI (8.73, 10.24), P < 0.00001], serum creatinine (Scr) [MD = -55.12, 95% CI (-63.42, -46.82), P < 0.00001], quantitative urine protein (Upro) [MD = -0.48, 95% CI (-0.53, -0.43), P < 0.00001], and blood urea nitrogen (BUN) [MD=-3.73, 95% CI (-4.08, -3.3) 7, P < 0.00001]. There was no statistical difference in the incidence of adverse reactions in each group. Conclusion: Currently, Shenkang injection combined with alprostadil has been widely used in clinical treatment of CRF due to the certain effect superior to other methods. However, its specific efficacy and safety need to be further verified through numerous large-scale clinical trials.

8.
Front Physiol ; 14: 1138947, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36969583

RESUMO

Background: The fruit of Terminalia chebula has been widely used for a thousand years for treating diarrhea, ulcers, and arthritic diseases in Asian countries. However, the active components of this Traditional Chinese medicine and their mechanisms remain unclear, necessitating further investigation. Objectives: To perform simultaneous quantitative analysis of five polyphenols in T. chebula and evaluate their anti-arthritic effects including antioxidant and anti-inflammatory activity in vitro. Materials and methods: Water, 50% water-ethanol, and pure ethanol were used as extract solvents. Quantitative analysis of gallic acid, corilagin, chebulanin, chebulagic acid, and ellagic acid in the three extracts was performed using high-performance liquid chromatography (HPLC). Antioxidant activity was assessed by the 2,2-diphenylpicrylhydrazyl (DPPH) radical-scavenging assay, and anti-inflammatory activity was evaluated by detecting interleukin (IL)-6 and IL-8 expression in IL-1ß-stimulated MH7A cells. Results: The 50% water-ethanol solvent was the optimal solvent yielding the highest total polyphenol content, and the concentrations of chebulanin and chebulagic acid were much higher than those of gallic acid, corilagin, and ellagic acid in the extracts. The DPPH radical-scavenging assay showed that gallic acid and ellagic acid were the strongest antioxidative components, while the other three components showed comparable antioxidative activity. As for the anti-inflammatory effect, chebulanin and chebulagic acid significantly inhibited IL-6 and IL-8 expression at all three concentrations; corilagin and ellagic acid significantly inhibited IL-6 and IL-8 expression at high concentration; and gallic acid could not inhibit IL-8 expression and showed weak inhibition of IL-6 expression in IL-1ß-stimulated MH7A cells. Principal component analysis indicated that chebulanin and chebulagic acid were the main components responsible for the anti-arthritic effects of T. chebula. Conclusion: Our findings highlight the potential anti-arthritic role of chebulanin and chebulagic acid from T. chebula.

9.
FASEB J ; 37(4): e22840, 2023 04.
Artigo em Inglês | MEDLINE | ID: mdl-36943397

RESUMO

Erdafitinib is a novel fibroblast growth factor receptor (FGFR) inhibitor that has shown great therapeutic promise for solid tumor patients with FGFR3 alterations, especially in urothelial carcinoma. However, the mechanisms of resistance to FGFR inhibitors remain poorly understood. In this study, we found Erdafitinib could kill cells by inducing incomplete autophagy and increasing intracellular reactive oxygen species levels. We have established an Erdafitinib-resistant cell line, RT-112-RS. whole transcriptome RNA sequencing (RNA-Seq) and Cytospace analysis performed on Erdafitinib-resistant RT-112-RS cells and parental RT-112 cells introduced P4HA2 as a linchpin to Erdafitinib resistance. The gain and loss of function study provided evidence for P4HA2 conferring such resistance in RT-112 cells. Furthermore, P4HA2 could stabilize the HIF-1α protein which then activated downstream target genes to reduce reactive oxygen species levels in bladder cancer. In turn, HIF-1α could directly bind to P4HA2 promoter, indicating a positive loop between P4HA2 and HIF-1α in bladder cancer. These results suggest a substantial role of P4HA2 in mediating acquired resistance to Erdafitinib and provide a potential target for bladder cancer treatment.


Assuntos
Carcinoma de Células de Transição , Neoplasias da Bexiga Urinária , Humanos , Carcinoma de Células de Transição/tratamento farmacológico , Carcinoma de Células de Transição/genética , Carcinoma de Células de Transição/patologia , Linhagem Celular Tumoral , Pirazóis/farmacologia , Espécies Reativas de Oxigênio , Receptor Tipo 3 de Fator de Crescimento de Fibroblastos/genética , Neoplasias da Bexiga Urinária/tratamento farmacológico , Neoplasias da Bexiga Urinária/genética , Neoplasias da Bexiga Urinária/patologia
10.
PLoS Genet ; 19(3): e1010319, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36976799

RESUMO

One of the most common cell shape changes driving morphogenesis in diverse animals is the constriction of the apical cell surface. Apical constriction depends on contraction of an actomyosin network in the apical cell cortex, but such actomyosin networks have been shown to undergo continual, conveyor belt-like contractions before the shrinking of an apical surface begins. This finding suggests that apical constriction is not necessarily triggered by the contraction of actomyosin networks, but rather can be triggered by unidentified, temporally-regulated mechanical links between actomyosin and junctions. Here, we used C. elegans gastrulation as a model to seek genes that contribute to such dynamic linkage. We found that α-catenin and ß-catenin initially failed to move centripetally with contracting cortical actomyosin networks, suggesting that linkage is regulated between intact cadherin-catenin complexes and actomyosin. We used proteomic and transcriptomic approaches to identify new players, including the candidate linkers AFD-1/afadin and ZYX-1/zyxin, as contributing to C. elegans gastrulation. We found that ZYX-1/zyxin is among a family of LIM domain proteins that have transcripts that become enriched in multiple cells just before they undergo apical constriction. We developed a semi-automated image analysis tool and used it to find that ZYX-1/zyxin contributes to cell-cell junctions' centripetal movement in concert with contracting actomyosin networks. These results identify several new genes that contribute to C. elegans gastrulation, and they identify zyxin as a key protein important for actomyosin networks to effectively pull cell-cell junctions inward during apical constriction. The transcriptional upregulation of ZYX-1/zyxin in specific cells in C. elegans points to one way that developmental patterning spatiotemporally regulates cell biological mechanisms in vivo. Because zyxin and related proteins contribute to membrane-cytoskeleton linkage in other systems, we anticipate that its roles in regulating apical constriction in this manner may be conserved.


Assuntos
Actomiosina , Caenorhabditis elegans , Animais , Actomiosina/genética , Actomiosina/metabolismo , Zixina/genética , Zixina/metabolismo , Caenorhabditis elegans/metabolismo , Constrição , Proteômica , Junções Intercelulares/genética , Junções Intercelulares/metabolismo , Morfogênese/genética
11.
Altern Ther Health Med ; 29(4): 52-56, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36947659

RESUMO

Context: Diabetic nephropathy (DN) is a common microvascular complication in diabetic patients. The pathogenesis of DN is complex. Inflammatory response may play a key role as a common downstream pathway. Objective: The study intended to explore the relationship between the levels of plasma nucleotide-binding oligomeric domain-like receptor protein 3 (NLRP3 inflammasome), interleukin-1ß (IL-1ß), and IL-18 and the progression of type 2 diabetic nephropathy to clarify their relationship with type 2 diabetes mellitus (T2DM) and to provide evidence for clinical treatment. Design: The research team performed a controlled observational study. Setting: The study took place at Baoding No. 1 Central Hospital in Baoding, Hebei, China. Participants: Participants were 153 patients with T2DM who received treatment at the hospital between October 2020 and October 2021. The research team allocated 30 participants without evidence of DN to the control group. Based on the DN stage, the team assigned the 123 remaining participants to one of five observation groups: (1) 32 participants with stage 1 DN to the DN1 group, (2) 31 participants with stage 2 DN to the DN2 group, (3) 30 participants with stage 3 DN to the DN3 group, (4) 30 participants with stage 4 DN to the DN4 group, and (5) 29 participants with stage 5 DN to the DN5 group. Outcome Measures: The research team measured participants' levels of "nucleotide binding oligomeric domain-like receptor protein 3" (NLRP3), interleukin-1 beta (IL-1ß), and IL-18 and used the Spearman rank correlation analysis to determine the correlation between those levels and the DN stages. Results: The levels of NLRP3 , IL-1ß and IL-18 in all the five observation groups were significantly higher than those in the control group (all P < .01). The levels were also significantly higher: (1) in the DN2, DN3, DN4, and DN5 groups than those in the DN1 group (all P < .01); (2) in the DN3, DN4, and DN5 groups than those in the DN2 group (all P < .01); (3) in the DN4 and DN5 groups than those in the DN3 group (all P < .01); and (4) in the DN5 groups than those in the DN4 group (all P < .01). The Spearman rank correlation analysis showed that the NLRP3, IL-1ß, and IL-18 levels were significantly positively correlated with the DN stage (P = .01). Conclusions: NLRP3, IL-1ß and IL-18 played an important role in the progression of T2DM, and their levels increased with the aggravation of DN. Therefore, the plasma levels of NLRP3, IL-1ß and IL-18 can be useful as indicators of the occurrence and development of DN and can provide clinical guidance for the early diagnosis of DN and for the determination and adjustment of treatment plans.


Assuntos
Diabetes Mellitus Tipo 2 , Nefropatias Diabéticas , Humanos , Nefropatias Diabéticas/metabolismo , Nefropatias Diabéticas/patologia , Proteína 3 que Contém Domínio de Pirina da Família NLR/análise , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Diabetes Mellitus Tipo 2/complicações , Interleucina-18/uso terapêutico , Interleucina-1beta/metabolismo , Interleucina-1beta/uso terapêutico , Nucleotídeos/uso terapêutico
12.
Nucleic Acids Res ; 51(8): e48, 2023 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-36999592

RESUMO

The ontogeny and dynamics of mtDNA heteroplasmy remain unclear due to limitations of current mtDNA sequencing methods. We developed individual Mitochondrial Genome sequencing (iMiGseq) of full-length mtDNA for ultra-sensitive variant detection, complete haplotyping, and unbiased evaluation of heteroplasmy levels, all at the individual mtDNA molecule level. iMiGseq uncovered unappreciated levels of heteroplasmic variants in single cells well below the conventional NGS detection limit and provided accurate quantitation of heteroplasmy level. iMiGseq resolved the complete haplotype of individual mtDNA in single oocytes and revealed genetic linkage of de novo mutations. iMiGseq detected sequential acquisition of detrimental mutations, including large deletions, in defective mtDNA in NARP/Leigh syndrome patient-derived induced pluripotent stem cells. iMiGseq identified unintended heteroplasmy shifts in mitoTALEN editing, while showing no appreciable level of unintended mutations in DdCBE-mediated mtDNA base editing. Therefore, iMiGseq could not only help elucidate the mitochondrial etiology of diseases, but also evaluate the safety of various mtDNA editing strategies.


Assuntos
DNA Mitocondrial , Genoma Mitocondrial , DNA Mitocondrial/genética , Heteroplasmia/genética , Genoma Mitocondrial/genética , Mitocôndrias/genética , Mutação
13.
Environ Sci Pollut Res Int ; 30(22): 62590-62601, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36947376

RESUMO

The corrosion behavior of Q235B carbon steel is investigated in water, LB medium, and oilfield produced water adding Pseudomonas fluorescens FSYZ01. After immersion at 30 °C for 13 days, the weight loss of carbon steel with this strain decreased by 32.23%, 54.07%, and 78.34%, respectively. X-ray diffraction (XRD) results show that P. fluorescens FSYZ01 inhibited conversion of iron oxides by hindering oxygen from approaching metal surface. Fourier transform infrared (FT-IR) and X-ray photoelectron spectrometer (XPS) results show that specific functional groups and bonds reacted with Fe(II/III) to form a dense and stable chelate-oxide protective layer, thereby inhibiting corrosion. Pyrolysis gas chromatography-mass spectrometer (Py-GCMS) results demonstrate the bacteria degraded C12 to C20 alkanes in oil. The inhibitory mechanism of crude oil-degrading bacteria P. fluorescens FSYZ01 on the carbon steel corrosion was proposed, so as to slow corrosion of oilfield produced water system pipeline and prolong its service life, helping to comprehend the microbial corrosion in the actual environment.


Assuntos
Pseudomonas fluorescens , Aço , Aço/química , Água , Campos de Petróleo e Gás , Biofilmes , Espectroscopia de Infravermelho com Transformada de Fourier , Corrosão , Carbono
14.
J Am Chem Soc ; 145(10): 5769-5777, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36863033

RESUMO

A series of novel surface Ru-H bipyridine complexes-grafted TiO2 nanohybrids were for the first time prepared by a combined procedure of surface organometallic chemistry with post-synthetic ligand exchange for photocatalytic conversion of CO2 to CH4 with H2 as electron and proton donors under visible light irradiation. The selectivity toward CH4 increased to 93.4% by the ligand exchange of 4,4'-dimethyl-2,2'-bipyridine (4,4'-bpy) with the surface cyclopentadienyl (Cp)-RuH complex and the CO2 methanation activity was enhanced by 4.4-fold. An impressive rate of 241.2 µL·g-1·h-1 for CH4 production was achieved over the optimal photocatalyst. The femtosecond transient IR absorption results demonstrated that the hot electrons were fast injected in 0.9 ps from the photoexcited surface 4,4'-bpy-RuH complex into the conduction band of TiO2 nanoparticles to form a charge-separated state with an average lifetime of ca. 50.0 ns responsible for the CO2 methanation. The spectral characterizations indicated clearly that the formation of CO2•- radicals by single electron reduction of CO2 molecules adsorbed on surface oxygen vacancies of TiO2 nanoparticles was the most critical step for the methanation. Such radical intermediates were inserted into the explored Ru-H bond to generate Ru-OOCH species and finally CH4 and H2O in the presence of H2.

15.
Genes (Basel) ; 14(3)2023 03 21.
Artigo em Inglês | MEDLINE | ID: mdl-36981030

RESUMO

(1) Background: MicroRNAs (miRNAs) are involved in a variety of biological processes, such as cell proliferation, cell differentiation, and organ development. Recent studies have shown that plant miRNAs may enter the diet and play physiological and/or pathophysiological roles in human health and disease; however, little is known about plant miRNAs in chickens. (2) Methods: Here, we analyzed miRNA sequencing data, with the use of five Chinese native chicken breeds and six different tissues (heart, liver, spleen, lung, kidney, and leg muscle), and used Illumina sequencing to detect the expression of plant miRNAs in the pectoralis muscles at fourteen developmental stages of Tibetan chickens. (3) Results: The results showed that plant miRNAs are detectable in multiple tissues and organs in different chicken breeds. Surprisingly, we found that plant miRNAs, such as tae-miR2018, were detectable in free-range Tibetan chicken embryos at different stages. The results of gavage feeding experiments also showed that synthetic tae-miR2018 was detectable in caged Tibetan chickens after ingestion. The analysis of tae-miR2018 showed that its target genes were related to skeletal muscle organ development, regulation of mesodermal cell fate specification, growth factor activity, negative regulation of the cell cycle, and regulation of growth, indicating that exogenous miRNA may regulate the development of chicken embryos. Further cell cultures and exogenous miRNA uptake assay experiments showed that synthetic wheat miR2018 can be absorbed by chicken myoblasts. (4) Conclusions: Our study found that chickens can absorb and deposit plant miRNAs in various tissues and organs. The plant miRNAs detected in embryos may be involved in the development of chicken embryos.


Assuntos
Galinhas , MicroRNAs , Humanos , Embrião de Galinha , Animais , Galinhas/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Melhoramento Vegetal , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Músculo Esquelético/metabolismo
16.
Adv Sci (Weinh) ; 10(13): e2207216, 2023 05.
Artigo em Inglês | MEDLINE | ID: mdl-36951540

RESUMO

Intervertebral disc degeneration (IVDD)-induced lower back pain (LBP) is a common problem worldwide. The underlying mechanism is partially accredited to ferroptosis, based on sequencing analyses of IVDD patients from the gene expression omnibus (GEO) databases. In this study, it is shown that polydopamine nanoparticles (PDA NPs) inhibit oxidative stress-induced ferroptosis in nucleus pulposus (NP) cells in vitro. PDA NPs scavenge reactive oxygen species (ROS), chelate Fe2+ to mitigate iron overload, and regulate the expression of iron storage proteins such as ferritin heavy chain (FHC), ferritin, and transferrin receptor (TFR). More importantly, PDA NPs co-localize with glutathione peroxidase 4 (GPX4) around the mitochondria and suppress ubiquitin-mediated degradation, which in turn exerts a protective function via the transformation and clearance of phospholipid hydroperoxides. PDA NPs further down-regulate malondialdehyde (MDA) and lipid peroxide (LPO) production; thus, antagonizing ferroptosis in NP cells. Moreover, PDA NPs effectively rescue puncture-induced degeneration in vivo by targeting ferroptosis and inhibiting GPX4 ubiquitination, resulting in the upregulation of antioxidant pathways. The findings offer a new tool to explore the underlying mechanisms and a novel treatment strategy for IVDD-induced LBP.


Assuntos
Ferroptose , Degeneração do Disco Intervertebral , Humanos , Fosfolipídeo Hidroperóxido Glutationa Peroxidase/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Degeneração do Disco Intervertebral/metabolismo , Ferro/metabolismo , Ubiquitinação
17.
Adv Sci (Weinh) ; 10(14): e2204438, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36965071

RESUMO

Chemoresistance is the main obstacle in osteosarcoma (OS) treatment; however, the underlying mechanism remains unclear. In this study, it is discovered that DDRGK domain-containing protein 1 (DDRGK1) plays a fundamental role in chemoresistance induced in OS. Bioinformatic and tissue analyses indicate that higher expression of DDRGK1 correlates with advanced tumor stage and poor clinical prognosis of OS. Quantitative proteomic analyses suggest that DDRGK1 plays a critical role in mitochondrial oxidative phosphorylation. DDRGK1 knockout trigger the accumulation of reactive oxygen species (ROS) and attenuate the stability of nuclear factor erythroid-2-related factor 2 (NRF2), a major antioxidant response element. Furthermore, DDRGK1 inhibits ubiquitin-proteasome-mediated degradation of NRF2 via competitive binding to the Kelch-like ECH-associated protein 1 (KEAP1) protein, which recruits NRF2 to CULLIN(CUL3). DDRGK1 knockout attenuates NRF2 stability, contributing to ROS accumulation, which promotes apoptosis and enhanced chemosensitivity to doxorubicin (DOX) and etoposide in cancer cells. Indeed, DDRGK1 knockout significantly enhances osteosarcoma chemosensitivity to DOX in vivo. The combination of DDRGK1 knockdown and DOX treatment provides a promising new avenue for the effective treatment of OS.


Assuntos
Fator 2 Relacionado a NF-E2 , Osteossarcoma , Humanos , Resistencia a Medicamentos Antineoplásicos , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteína 1 Associada a ECH Semelhante a Kelch/genética , Proteína 1 Associada a ECH Semelhante a Kelch/metabolismo , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Osteossarcoma/tratamento farmacológico , Proteômica , Espécies Reativas de Oxigênio/metabolismo , Ubiquitinação
18.
Huan Jing Ke Xue ; 44(3): 1319-1327, 2023 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-36922193

RESUMO

A total of 98 samples were collected to analyze the seasonal variation and source apportionment of carbonaceous components, especially brown carbon (BrC), of PM2.5in Luoyang during 2018-2019. The concentrations of organic carbon (OC) and elemental carbon (EC) ranged from (7.04±1.82) µg·m-3to(23.81±8.68) µg·m-3and (2.96±1.4) µg·m-3to (13.41±7.91) µg·m-3, respectively, showing the seasonal variation of being high in winter and low in summer; the carbonaceous fraction and secondary organic aerosol percentages were higher by 8.33%-141.03% and by 0.77%-63.14%, respectively, compared with that in 2015. The light absorption cross section (MAC) values showed different seasonal variations with the concentration of carbonaceous fraction, shown in descending order as autumn (7.67 m2·g-1)>winter (5.65 m2·g-1)>spring (5.13 m2·g-1)>summer (3.84 m2·g-1). The MAC values ranged from 3.84 to 7.67 m2·g-1 at 445 nm, which was lower than that in coal ash. Seasonal variation in light absorption and the contribution of BrC to total light absorption (babs,BrC,405 nm, babs,BrC,405 nm/babs,405 nm) in descending order was winter (31.57 Mm-1, 33%), autumn (11.40 Mm-1, 25%), spring (4.88 Mm-1, 23%), and summer (2.12 Mm-1, 21%). The proportion of carbonaceous components decreased as haze episodes evolved, whereas the contribution of light absorption of BrC increased, highlighting the important contribution of BrC to the total light absorption. The results of PMF and correlation coefficients of babs,BrC,405 nm and PM2.5 components indicated that motor vehicles and secondary nitrate contributed 27.7% and 24.0%, respectively. Our findings have significant scientific implications for the deep controlling of carbonaceous aerosol, especially for BrC, in Luoyang in the future.

19.
Sensors (Basel) ; 23(5)2023 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-36904811

RESUMO

How to predict precipitation accurately and efficiently is the key and difficult problem in the field of weather forecasting. At present, we can obtain accurate meteorological data through many high-precision weather sensors and use them to forecast precipitation. However, the common numerical weather forecasting methods and radar echo extrapolation methods have insurmountable defects. Based on some common characteristics of meteorological data, this paper proposes a Pred-SF model for precipitation prediction in target areas. The model focuses on the combination of multiple meteorological modal data to carry out self-cyclic prediction and a step-by-step prediction structure. The model divides the precipitation prediction into two steps. In the first step, the spatial encoding structure and PredRNN-V2 network are used to construct the autoregressive spatio-temporal prediction network for the multi-modal data, and the preliminary predicted value of the multi-modal data is generated frame by frame. In the second step, the spatial information fusion network is used to further extract and fuse the spatial characteristics of the preliminary predicted value and, finally, output the predicted precipitation value of the target region. In this paper, ERA5 multi-meteorological mode data and GPM precipitation measurement data are used for testing to predict the continuous precipitation of a specific area for 4 h. The experimental results show that Pred-SF has strong precipitation prediction ability. Some comparative experiments were also set up for comparison to demonstrate the advantages of the combined prediction method of multi-modal data and the stepwise prediction method of Pred-SF.

20.
bioRxiv ; 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36778218

RESUMO

Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical constriction are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In the Drosophila ventral furrow, the medioapical actomyosin network has a sarcomere-like architecture, with radially polarized actin filaments and centrally enriched non-muscle myosin II and myosin activating kinase. To determine if this is a broadly conserved actin architecture driving apical constriction, we examined actomyosin architecture during C. elegans gastrulation, in which two endodermal precursor cells internalize from the surface of the embryo. Quantification of protein localization showed that neither the non-muscle myosin II NMY-2 nor the myosin-activating kinase MRCK-1 is enriched at the center of the apex. Further, visualization of barbed- and pointed-end capping proteins revealed that actin filaments do not exhibit radial polarization at the apex. Taken together with observations made in other organisms, our results demonstrate that diverse actomyosin architectures are used in animal cells to accomplish apical constriction. Summary: Through live-cell imaging of endogenously-tagged proteins, Zhang, Medwig-Kinney, and Goldstein show that the medioapical actomyosin network driving apical constriction during C. elegans gastrulation is organized diffusely, in contrast to the sarcomere-like architecture previously observed in the Drosophila ventral furrow.

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