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1.
J Colloid Interface Sci ; 559: 65-75, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31610306

RESUMO

Electroactive nanofibrous scaffold is a vital tool for the study of the various biological research fields from bioelectronics to regenerative medicine, which can provide cell preferable 3D nanofiber architecture and programmed electrical signal. However, intrinsic non-biodegradability is a major problem that hinders its widespread application in the clinic. Herein, we designed, synthesized, and characterized shell/core poly (3,4-ethylenedioxythiophene) (PEDOT)/chitosan (CS) nanofibers by combining the electrospinning and recrystallization processes. Upon incorporating a trace amount of PEDOT (1.0 wt%), the resultant PEDOT/CS nanofibers exhibited low interfacial charge transfer impedance, high electrochemical stability, high electrical conductivity (up to 0.1945 S/cm), and ultrasensitive piezoelectric property (output voltage of 22.5 mV by a human hair prodding). With such unique electrical and conductive properties, PEDOT/CS nanofibers were further applied to brain neuroglioma cells (BNCs) to stimulate their adhesion, proliferation, growth, and development under an optimal external electrical stimulation (ES) and a pulse voltage of 400 mV/cm. ES-responsive PEDOT/CS nanofibers indeed promoted BNCs growth and development as indicated by a large number and density of axons. The synergetic interplay between external ES and piezoelectric voltage demonstrates new PEDOT-based nanofibers as implantable electroactive scaffolds for numerous applications in nerve tissue engineering, human health monitoring, brain mantle information extraction, and degradable microelectronic devices.

2.
IEEE Trans Image Process ; 29: 85-99, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31329557

RESUMO

Superpixel segmentation of 2D images has been widely used in many computer vision tasks. Previous algorithms model the color, position, or higher spectral information for segmenting a 2D image. However, limited to the Gaussian imaging principle in a traditional camera, where each pixel is formed by summing lots of light rays from different angles, there is not a thorough segmentation solution to eliminate the ambiguity in defocus and occlusion boundary areas. In this paper, we consider the essential element of image pixel, i.e., rays in light space, and propose light field superpixel (LFSP) to eliminate the ambiguity. The LFSP is first defined mathematically and then two evaluation metrics, named LFSP self-similarity and effective label ratio, are proposed to evaluate the refocus-invariant and full-sliced properties of segmentation. By building a clique system containing 80 neighbors in light field, a robust refocus-invariant LFSP segmentation algorithm is developed. Experimental results on both synthetic and real light field datasets demonstrate the advantages over the current state of the art in terms of traditional evaluation metrics. Additionally, the LFSP self-similarity evaluations under different light field refocus levels show the refocus-invariance of the proposed algorithm. The full-sliced property of the proposed LFSP algorithm is verified by comparing it with the classical supervoxel algorithms. Finally, an LFSP-based application is demonstrated to show the effectiveness of LFSP in light field editing.

3.
Virus Res ; 275: 197754, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31622636

RESUMO

Herpesvirus infection usually relies on the interaction between viral protein and host protein to enhance replication of the enveloped virus. Fish Carassius auratus herpesvirus (CaHV) is highly pathogenic pathogen causing gill acute hemorrhages of crucian carp (Carassius auratus) and high moritality rates among those infected fish. The protein of CaHV (CaHV-138 L) containing two transmembrane (TM) domains and an immunoglobulin C-2 Type (IGc2) domain was predicted as a viral membrane protein. In this investigation, fluorescence observation showed that full-length CaHV-138 L mainly localized on the plasma membrane or around nuclear membrane of fish fathead minnow (FHM) cells in a punctate pattern. The TM domain deletion mutants of CaHV-138 L (ΔTM1, ΔTM2, and ΔTM1&ΔTM2) diffusely distributed in both the cytoplasm and the nucleus, mainly presented patchy fashion in the cytoplasm, and mainly presented both in the nucleus and in the cytoplasm, respectively. Obviously, the TM domain deletion mutants significantly affected CaHV-138 L subcellular localization. Meanwhile, colocalization assay showed that the full-length viral protein colocalized with mitochondria. Furthermore, the interaction between CaHV-138 L and host protein was identified by yeast two-hybrid (Y2H) and co-immunoprecipitation (co-IP) assays. The host mitochondrial protein FoF1 ATP synthase (FoF1-ATPase) that interacts with this viral protein was screened. The data indicated that CaHV-138 L can target to mitochondrial protein FoF1-ATPase, which might provide energy for virus replication through mediating mitochondrial ATP synthesis. This study has provided valuable information for better understanding of the links of herpesvirus proteins with aquaculture animal proteins.

4.
J Cell Biochem ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31674056

RESUMO

Recently, microRNAs (miRNAs) receive more attention due to their role in the pathogenesis of malignancies. Retinoblastoma (RB) is the most serious and harmful malignant tumor in infants and young children with eye diseases, which often endangers the lives of children. This study was designed to determine how miR-598 is involved in RB progression. In this study, quantitative reverse transcription-polymerase chain reaction, Western blot, dual-luciferase reporter, Cell Counting Kit-8, and Transwell assays were adopted to detect miR-598 expression and function in RB. The decreased expression of miR-598 was identified in RB. Overexpression of miR-598 suppressed the viability and metastasis of RB cells. Further, E2F transcription factor 1 (E2F1) is verified as a direct target of miR-598. Furthermore, E2F1 recovered miR-598-mediated-inhibition of cell viability and metastasis in RB. In addition, miR-598 was found to promote cell apoptosis and inactivate the protein kinase B (AKT) pathway in RB. miR-598 suppressed RB cell viability and metastasis through inhibiting E2F1 and inactivating AKT pathway, which may provide a new perspective for RB treatment.

5.
Appl Opt ; 58(28): 7688-7692, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31674449

RESUMO

In order to realize the function of subdiffraction focusing using a single flat lens, a special metalens is proposed to modulate the circularly polarized light. Initially, the analyses indicate that the phase shift can cover [0,2π] by changing the rotation angle of a quadrangular-frustum pyramid-shaped structure from 0° to 180°, while the average amplitude transmittance can reach 96% at the wavelength of λ=1550 nm. Then, a flat metalens is designed by carefully arranging the quadrangular-frustum pyramid-shaped structures. The simulated results show that a focal spot could be obtained at the focal length of about f=2.87λ, and the focusing efficiency is ∼14.9%. Meanwhile, the full width at half maximum (FWHM) of the focusing spot is only 0.48λ, which is smaller than the diffraction limit. Furthermore, this designed metalens can function comparatively with a conventional lens in one-to-one imaging.

6.
ACS Nano ; 13(11): 13513-13523, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31714743

RESUMO

Red phosphorus (P) has been recognized as a promising material for lithium/sodium-ion batteries (LIBs/SIBs) because of their high theoretical capacity. However, tremendous volume variation and low conductivity limit its widespread applications. Hence, we design and synthesize uniformly distributed honeycomb-like hierarchical micro-mesoporous carbon nanospheres (HHPCNSs) with ultralarge pore volume (3.258 cm3 g-1) on a large scale through a facile way. The large pore volume provides enough space for loading of P and the expansion of P, and the uniform distribution of the micro-mesopores enables the red P to load uniformly. The resulting HHPCNSs/P composite exhibits extremely high capacity (2463.8 and 2367.6 mA h g-1 at 0.1 A g-1 for LIBs and SIBs, respectively), splendid rate performance (842.2 and 831.1 mA h g-1 at 10 A g-1 for LIBs and SIBs, respectively) and superior cycling stability (1201.6 and 938.4 mA h g-1 at 2 and 5 A g-1 after 1000 cycles for LIBs and 1269.4 and 861.8 mA h g-1 at 2 and 5 A g-1 after 1000 cycles for SIBs, respectively). More importantly, when coupled with LiFePO4 and Na3V2(PO4)3 cathode, lithium/sodium-ion full batteries display high capacity and superior rate and cycling performances, revealing the practicability of the HHPCNSs/P composite. The exceptional electrochemical performance is caused by the honeycomb-like carbon network with ultralarge pore volume, uniformly distributed hierarchical micro-mesoporous nanostructure, outstanding electronic conductivity, and excellent nanostructural stability, which is much better than currently reported P/C materials for both LIBs and SIBs.

7.
Acta Trop ; 202: 105246, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31672488

RESUMO

Oriental liver fluke disease (clonorchiasis), caused by Clonorchis sinensis, is endemic in 81 counties in Guangdong province, China. This study was conducted following increasing lack of clonorchiasis awareness and increased consumption of raw freshwater fish and raw fish porridge at home and restaurants, which has been attributed to improved living standards in China, and is considered to have led to a higher C. sinensis infection rate in the country. A total of 61,517 individuals were investigated from 123 sites during a provincial survey in 1988, with average C. sinensis infection rate of 1.82% that increased to 4.08% (335/8217) in 1997. Higher infection rate was observed in adult men than women, with heavy infection rate in fishermen and businessmen because they have access and means to get raw fishes for consumption. Thus, clonorchiasis has become a serious public health problem in Guangdong province. Efforts targeting clonorchiasis prevention and control in Guangdong province started in the 1990s. These included carrying out large-scale surveys and chemotherapy in epidemic regions, research on promotion of environmental sanitation and methods for freshwater aquaculture. Establishment and promotion of clonorchiosis demonstration plots for comprehensive control measures, as well as the development of clonorchiasis-prevention community-out-patient clinics were initiated and their effectiveness was evaluated. However, there seem to be no obvious decline in the prevalence of C. sinensis with 4.90% (608/12,401) in 2015, at Guangdong province due to the people's habit of eating raw fishes in endemic areas. This has led to increasing calls to improve environmental sanitation in the aquaculture industry. It is, however, believed that control efforts on clonorchiosis in the province are at an early stage and require strengthening through the cooperation of different departments to work out effective strategies for sustainable field application of control mechanisms.

8.
Immunol Lett ; 217: 72-76, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31730775

RESUMO

Severe acute pancreatitis (SAP) has a complex course and a worse prognosis. Immune response imbalance is an important cause of severe pancreatitis or even death in patients. Immunomodulation therapy can regulate the imbalance of inflammatory response, alleviate SAP-related organ damage and improve the prognosis of patients. There are some problems in early immune regulation measures, such as single target and simple way. In recent years, new treatment methods, such as regulating the maturation and apoptosis of immune cells, the application of mesenchymal stem cells (MSCs) and multifactor combination therapy, have provided new ideas and hope for the future treatment of SAP. This article reviews the development of SAP immunoregulation and its recent progress.

9.
Bone ; 131: 115151, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31751752

RESUMO

Dentin matrix protein 1 (DMP1) is primarily expressed in osteocytes, although a low level of DMP1 is also detected in chondrocytes. Removing Dmp1 in mice or a mutation in humans leads to hypophosphatemic rickets (identical to X-linked hypophosphatemia). The deformed skeletons were currently thought to be a consequence of an inhibition of chondrogenesis (leading to an accumulation of hypertrophic chondrocytes and a failure in the replacement of cartilage by bone). To precisely study the mechanisms by which DMP1 and phosphorus control temporomandibular condyle formation, we first showed severe malformed condylar phenotypes in Dmp1-null mice (great expansions of deformed cartilage layers and subchondral bone), which worst as aging. Next, we excluded the direct role of DMP1 in condylar hypertrophic-chondrogenesis by conditionally deleting Dmp1 in hypertrophic chondrocytes using Col10a1-Cre and Dmp1 loxP mice (displaying no apparent phosphorous changes and condylar phenotype). To address the mechanism by which the onset of endochondral phenotypes takes place, we generated two sets of tracing lines in the Dmp1 KO background: AggrecanCreERT2-ROSA-tdTomato and Col 10a1-Cre-ROSA-tdTomato, respectively. Both tracing lines displayed an acceleration of chondrogenesis and cell trans-differentiation from chondrocytes into bone cells in the Dmp1 KO. Next, we showed that administrations of neutralizing fibroblast growth factor 23 (FGF23) antibodies in Dmp1-null mice restored hypophosphatemic condylar cartilage phenotypes. In further addressing the rescue mechanism, we generated compound mice containing Col10a1-Cre with ROSA-tdTomato and Dmp1 KO lines with and without a high Pi diet starting at day 10 for 39 days. We demonstrated that hypophosphatemia leads to an acceleration of chondrogenesis and trans-differentiation of chondrocytes to bone cells, which were largely restored under a high Pi diet. Finally, we identified the causative molecule (ß-catenin). Together, this study demonstrates that the Dmp1-null caused hypophosphatemia, leading to acceleration (instead of inhibition) of chondrogenesis and bone trans-differentiation from chondrocytes but inhibition of bone cell maturation due to a sharp increase in ß-catenin. These findings will aid in the future treatment of hypophosphatemic rickets with FGF23 neutralizing antibodies.

10.
J Clin Lab Anal ; : e23097, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31774228

RESUMO

BACKGROUND: This study aimed to explore the potential of soluble urokinase plasminogen activator receptor (suPAR) as a biomarker for severe acute pancreatitis (SAP) risk prediction and disease management in SAP patients. METHODS: Totally 225 acute pancreatitis (AP) patients (including 75 SAP, 75 moderate-severe acute pancreatitis [MSAP], and 75 mild acute pancreatitis [MAP] patients) were recruited based on the Atlanta classification, and their serum samples were obtained within 24 hours after admission. Meanwhile, 75 health controls (HCs) were recruited with their serum samples collected at the enrollment. The serum suPAR was then detected using enzyme-linked immunosorbent assay. RESULTS: The suPAR level was increased in SAP patients compared with MSAP patients (P = .023), MAP patients (P < .001), and HCs (P < .001). Receiver operating characteristic (ROC) curve presented that suPAR could not only differentiate SAP patients from HCs (AUC: 0.920, 95%CI: 0.875-0.965) but also differentiate SAP patients from MSAP (AUC: 0.684, 95%CI: 0.600-0.769) and MAP patients (AUC: 0.855, 95%CI: 0.797-0.912). In SAP patients, suPAR was positively correlated with Ranson score (P < .001), acute physiology and chronic healthcare evaluation II score (P = .001), sequential organ failure assessment score (P < .001), and C-reaction protein (P = .002). Further ROC curve exhibited that suPAR (AUC: 0.806, 95%CI: 0.663-0.949) was of good value in predicting increased inhospital mortality of SAP patients. CONCLUSION: Soluble urokinase plasminogen activator receptor is of good predictive value for SAP risk and may serve as a potential biomarker for disease severity, inflammation, and inhospital mortality in SAP patients.

11.
Scand Cardiovasc J ; : 1-5, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31777288

RESUMO

Objective: The purpose of this study was to explore the different provocative maneuvers in detecting right-to-left shunt during contrast transthoracic echocardiography (cTTE). Methods: We evaluated 378 patients for right-to-left shunt detection by cTTE at the quiet respiration, during Valsalva maneuver (blowing into a face mask connected to a sphygmomanometer at 40 mmHg for 10 s), and during cough maneuver, performed in random order, and the degree of right-to-left shunt as the number of microbubbles in the left atrium per frame after each provocative maneuver was recorded. Results: The detection rates of right-to-left shunt for quiet respiration, Valsalva maneuver, and cough maneuver were 16%, 33%, and 38%, respectively (p < .001). The detection rate for cough maneuver was significantly higher than that for Valsalva maneuver (p < .001). There was no significant difference between Valsalva maneuver and cough maneuver in terms of detecting moderate- or severe-extent right-to-left shunt (p > .05), but cough maneuver was significantly better than Valsalva maneuver at detecting mild-extent right-to-left shunt (p = .001). Conclusions: Cough maneuver is an effective alternative to Valsalva maneuver, with a higher detection rate for mild-extent right-to-left shunt during cTTE, maybe due to its own mechanical characteristics.

12.
Microbiol Res ; 231: 126370, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31739261

RESUMO

The role of the gut microbiome in animal health has become increasingly evident. Although the structure of the gut microbiome of A. mellifera is well known, little is known about the dynamic change across different developmental stages. In this study, we explored the dynamic changes of the gut microbiota of A. mellifera at different developmental stages covering the whole life cycle using high-throughput 16S rRNA gene sequencing. The results indicated that the core (shared) gut microbiota changes significantly among different developmental stages. The diversity of the bacterial community in workers among different ages was significantly different. In addition, by comparing the core gut microbiota among different-aged workers, we found that newly emerged workers had fewer core microbiota. Three genera, Gilliamella, Frischella, and Snodgrassella, were significantly colonized at 1 day poste mergence (dpe); Lactobacillus, Bifidobacterium, Commensalibacter were significantly colonized at 3 dpe and significantly reduced with Gilliamella. Lactobacillus kunkeei and Bartonella were significantly colonized at 12 dpe and were significantly decreased with Lactobacillus helsingborgensis. Commensalibacter and Bifidobacterium were significantly decreased at 25 dpe, and Bacteroides, Escherichia-Shigella, and Porphyromonadaceae were significantly decreased between 19 and 25 dpe. Our results reveal the succession of the gut microbiota of workers from birth to senescence, which provides a theoretical basis for further exploring the roles of gut microbiota during different developmental stages.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31770344

RESUMO

STUDY DESIGN: This is an in vitro biomechanical study. OBJECTIVE: This study aimed to investigate the biomechanical variations of lumbar spine motor units after bilateral facet joint severe violation in cadaver specimens and analyze the biomechanics under different moments. SUMMARY OF BACKGROUND DATA: The incidence of facet joint violation (FJV) is highly variable, and one of the most important factors is the lack of awareness of protection. Until now, the biomechanical effects of FJV remains unclear. METHODS: Biomechanical testing was performed on 12 human cadaveric spines under flexion-extension, lateral bending, and axial rotation loading. After intact analysis, pedicle screws were inserted at L5, and the biomechanical testing was repeated. Full range of motion (ROM) at the proximal adjacent levels under different moments was recorded and normalized to the intact (100%) noninstrumented spine. The relative ROM changes were compared between the control and severe violation groups. RESULTS: The adjacent-level ROM (flexion-extension, lateral bending, axial rotation) did not change significantly in the control group at each moment (7.5, 6.0, 4.5 N m) compared with the intact noninstrumented spine. In the severe violation group, the supradjacent-level ROM decreased significantly under all moments relative to the intact noninstrumented spine (P < 0.05) except for the ROM of lateral bending at moments of 7.5 and 6.0 N m. When comparing the ROM between the two groups, there were significant differences in all movements except lateral bending at 7.5 N m. CONCLUSIONS: When superior-segment bilateral facet joints are severely violated by screws, the flexion-extension and axial rotation ROM of adjacent vertebrae decreases at each moment (7.5, 6.0, 4.5 N m), and the lateral bending ROM decreases at 4.5 N m. LEVEL OF EVIDENCE: N/A.

14.
Sci Adv ; 5(11): eaax0629, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31763448

RESUMO

The mechanisms that drive programmed resolution of inflammation remain elusive. Here, we report the temporal regulation of soluble (s) and transmembrane (m) fibrinogen-like protein 2 (Fgl2) during inflammation and show that both sFgl2 and mFgl2 correlate with the outcome. The expression and ectodomain shedding of Fgl2 are respectively promoted by miR-466l and metalloproteinases (ADAM10 and ADAM17) during inflammation resolution. Deficiency of Fgl2 enhances polymorphonuclear neutrophil (PMN) infiltration but impairs macrophage (MΦ) maturation and phagocytosis and inhibits the production of n-3 docosapentaenoic acid-derived resolvin 5 (RvDp5). In contrast, administration of sFgl2 blunts PMN infiltration as well as promotes PMN apoptosis and RvDp5 biosynthesis. By activating ALX/FPR2, RvDp5 enhances sFgl2 secretion via ADAM17 and synergistically accelerates resolution of inflammation. These results uncover a previously unknown endogenous programmed mechanism by which Fgl2 regulates resolution of inflammation and shed new light on clinical sepsis treatments.

15.
Am Surg ; 85(11): 1288-1293, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31775973

RESUMO

Portal vein system thrombosis (PVST) is a form of venous thrombosis, which behaves as a common and potentially life-threatening complication after splenectomy. Numerous studies have been made to investigate the mechanism and the risk factors of PVST. However, the research on the prevention and treatment of PVST associated with platelet's (PLT) rule of change is rare. This work mainly investigates the PLT's rule of change and its correlativity with the formation of PVST after splenectomy. The retrospective study included 117 patients who underwent splenectomy from August 2014 to June 2018 and monitored by blood routine, D-dimer (D-D), and portal vein system ultrasound routinely after splenectomy. The changes of PLT and D-D were recorded and compared before and after each operation. We analyzed the changes of PLT and D-D as well as the incidence of PVST postoperatively. Most of the patients' PLT increased significantly after splenectomy. On the first day after operation, there were 80 patients' (68.38%) PLT recovered to normal or above normal. The proportion of patients with abnormally high PLT value on the seventh and fourteenth day were 60.68 per cent and 81.20 per cent, respectively. Thereinto, there were 67 patients' (57.26%) PLT beyond 500 × 109/L on the 14th day. The D-D of all patients exceeded the normal range on the first, seventh, and fourteenth day after operation, and the difference was statistically significant (P < 0.05), which behaved similar like PLT. Meanwhile, our color Doppler ultrasonography showed that the incidence of PVST was 70.79 per cent. Among the patients with PVST, 82 (100%) patients' PLT was increased after operation. The PLT of patients increased obviously after splenectomy. The increased blood viscosity caused by the consecutive elevation of PLT may result in a higher PVST incidence. Early intervention and treatment are needed clinically for PVST after splenectomy.

16.
Invest Ophthalmol Vis Sci ; 60(14): 4820-4829, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31747682

RESUMO

Purpose: This study was aimed at investigating the association of long noncoding RNA (lncRNA)-related single nucleotide polymorphisms (SNPs) with Vogt-Koyanagi-Harada (VKH) disease. Methods: LncRNA-related SNPs were selected by multi-omics analysis. Genotyping, expression of lncRNA and mRNA, cell proliferation, and cytokine production were tested by MassARRAY System, real-time PCR, CCK8, and ELISA. Results: A significant association with VKH was found for lnc-TOR3A-1:1/rs3829794, which is located in a non-HLA region (CC genotype: Bonferroni corrected P values [PC] = 2.98 × 10-8, odds ratio [OR] = 0.62; TT genotype: PC = 1.64 × 10-8, OR = 1.57; C allele: PC = 1.39 × 10-12, OR = 0.71). Additionally, an association was found for four lncRNA SNPs located in the HLA region. Functional experiments in rs3829794 genotyped individuals showed decreased ABL2 (ABL proto-oncogene 2, nonreceptor tyrosine kinase) expression, decreased proliferation of anti-CD3 plus anti-CD28-stimulated peripheral blood mononuclear cells (PBMCs), and an increased production of IL-10 in CC carriers compared to TT carriers (P = 0.0073, P = 0.0011, and P = 0.002, respectively). Conclusions: Our study identified five new loci associated with VKH susceptibility and identified a functional variant (lnc-TOR3A-1:1/rs3829794) that confers risk for VKH, which is possibly mediated by modulating gene expression, proliferation of lymphocytes, and regulation of anti-inflammatory cytokine production.

17.
J Cell Mol Med ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31749326

RESUMO

Cardiomyocyte apoptosis is an important process occurred during cardiac ischaemia-reperfusion injury. Long non-coding RNAs (lncRNA) participate in the regulation of various cardiac diseases including ischaemic reperfusion (I/R) injury. In this study, we explored the potential role of lncRNA ACART (anti-cardiomyocyte apoptosis-related transcript) in cardiomyocyte injury and the underlying mechanism for the first time. We found that ACART was significantly down-regulated in cardiac tissue of mice subjected to I/R injury or cultured cardiomyocytes treated with hydrogen peroxide (H2 O2 ). Knockdown of ACART led to significant cardiomyocyte injury as indicated by reduced cell viability and increased apoptosis. In contrast, overexpression of ACART enhanced cell viability and reduced apoptosis of cardiomyocytes treated with H2 O2 . Meanwhile, ACART increased the expression of the B cell lymphoma 2 (Bcl-2) and suppressed the expression of Bcl-2-associated X (Bax) and cytochrome-C (Cyt-C). In addition, PPAR-γ was up-regulated by ACART and inhibition of PPAR-γ abolished the regulatory effects of ACART on cell apoptosis and the expression of Bcl-2, Bax and Cyt-C under H2 O2 treatment. However, the activation of PPAR-γ reversed the effects of ACART inhibition. The results demonstrate that ACART protects cardiomyocyte injury through modulating the expression of Bcl-2, Bax and Cyt-C, which is mediated by PPAR-γ activation. These findings provide a new understanding of the role of lncRNA ACART in regulation of cardiac I/R injury.

18.
J Environ Manage ; 254: 109788, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31698299

RESUMO

Sepiolite (SEP) is a clay mineral with great potential to stabilize soil heavy metals. A two-year field experiment was conducted to explore the optimum use of SEP to immobilize soil Cd and to promote the consumption safety of rice grown in a typical paddy field in Southern China. SEP was applied once or twice over the two-year study at three levels (0.1, 0.5, and 1%, w/w) before rice planting. The results showed that SEP effectively reduced rice grain Cd concentrations by 47-49% in the first year and by 44-50% in the second year due to the residue effect. Application of SEP for two consecutive years reduced the rice grain Cd concentration by up to 75%, achieving a safe level (<0.2 mg kg-1). SEP also reduced Zn concentrations in rice grains (by 6-10%), while the Cd/Zn ratios of rice grains were decreased by 24-72% over the two years, implying it was much safer for consumption. SEP significantly increased the soil pH (0.9-1.8 units) and available phosphorus, and it reduced the soil available Cd (by 20-95%) and Zn concentrations (by 30-99%). In brief, SEP effectively stabilized soil Cd and reduced uptake by rice; the effect was dose-dependent and 0.5% (w/w) was optimum in the present study. The main mechanism of SEP to stabilize soil Cd is the increase in soil pH analogous to liming. This study shows that SEP application can be an efficient way to remediate Cd contaminated rice paddies and fulfill the goal of safe production of rice and thereby reduce the health risks associated with consuming rice.

19.
Biomed Tech (Berl) ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31743102

RESUMO

B-mode ultrasonography and sonoelastography are used in the clinical diagnosis of prostate cancer (PCa). A combination of the two ultrasound (US) modalities using computer aid may be helpful for improving the diagnostic performance. A technique for computer-aided diagnosis (CAD) of PCa is presented based on multimodal US. Firstly, quantitative features are extracted from both B-mode US images and sonoelastograms, including intensity statistics, regional percentile features, gray-level co-occurrence matrix (GLCM) texture features and binary texture features. Secondly, a deep network named PGBM-RBM2 is proposed to learn and fuse multimodal features, which is composed of the point-wise gated Boltzmann machine (PGBM) and two layers of the restricted Boltzmann machines (RBMs). Finally, the support vector machine (SVM) is used for prostatic disease classification. Experimental evaluation was conducted on 313 multimodal US images of the prostate from 103 patients with prostatic diseases (47 malignant and 56 benign). Under five-fold cross-validation, the classification sensitivity, specificity, accuracy, Youden's index and area under the receiver operating characteristic (ROC) curve with the PGBM-RBM2 were 87.0%, 88.8%, 87.9%, 75.8% and 0.851, respectively. The results demonstrate that multimodal feature learning and fusion using the PGBM-RBM2 can assist in the diagnosis of PCa. This deep network is expected to be useful in the clinical diagnosis of PCa.

20.
Histol Histopathol ; : 18186, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31745968

RESUMO

Gallbladder cancer (GBC) is a malignant tumor of the biliary tract. The main problem affecting the treatment of gallbladder cancer is late diagnosis and poor prognosis. EIF5A2 is one of two isoforms of the EIF5A family and is reported to be a new oncogenic protein in many human cancers. In this study, our results showed for the first time that EIF5A2 was overexpressed in GBC samples compared with non-tumor tissue. Overexpression of EIF5A2 was associated with lymph node metastasis, tumor differentiation, UICC (Union for International Cancer Control) staging, histological type, metastasis, and tumor size. Overexpression of EIF5A2 in gallbladder carcinoma tissues is also associated with poor prognosis in patients. The interference of EIF5A2 significantly inhibited the proliferation, cell cycle, migration and colony formation of GBC-SD cells in vitro. Our results suggest that EIF5A2 is a target oncogene and may be an important prognostic biomarker in the pathogenesis of gallbladder cancer.

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