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1.
Int J Hyg Environ Health ; 223(1): 56-64, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31722832

RESUMO

BACKGROUND: Point sources such as wastewater treatment plants (WWTPs) commonly discharge their effluent into rivers. Their waste may include antibiotic residues, disinfectants, antibiotic resistant bacteria (ARB), and Antimicrobial Resistance Genes (ARG). There is evidence that ARG can be found in the natural environment, but attribution to specific point sources is lacking. OBJECTIVES: The goal of this study was to assess the release and dissemination of ARG from three WWTPs in southern Chile via two pathways: through the river systems, and through wild birds. METHODS: A longitudinal study was conducted, collecting river sediment samples at different distances both upstream and downstream from each WWTP. Wild birds were sampled from around one of the WWTPs once a month for 13 months. A microfluidic q-PCR approach was used to quantify 48 genes covering different molecular mechanisms of resistance, and data was analyzed using ordination methods and linear mixed regression models. RESULTS: There was a statistically significant increase downstream from the WWTPs (p < 0.05) for 17 ARG, but the downstream dissemination through the rivers was not clear. Beta-lactamase genes blaKPC, blaTEM, and blaSHV were the most abundant in birds, with higher abundance of blaSHV in migratory species compared to resident species (p < 0.05). The gene profile was more similar between the migratory and resident bird groups compared to the WWTP gene profile. CONCLUSIONS: While results from this study indicate an influence of WWTPs on ARG abundance in the rivers, the biological significance of this increase and the extent of the WWTPs influence are unclear. In addition, wild birds were found to play a role in disseminating ARG, although association to the specific WWTP could not be ascertained.

2.
J Colloid Interface Sci ; 559: 206-214, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31629274

RESUMO

Controllable synthesis of multimetal nanocrystals with hierarchical structures and tunable compositions are feasible to steeply improve the catalytic properties in fuel cells. Herein, trimetallic PtRhCo petal-assembled alloyed nanoflowers (PtRhCo PAANFs) were fabricated via a one-pot solvothermal method, which showed remarkable enlargement in specific activity and mass activity over PtRh0.25Co nanodentrites (NDs), PtRh1.5Co NDs, PtCo NDs and commercial Pt/C catalysts for ethylene glycol oxidation in 0.5 M KOH solution. The as-developed catalyst exhibited dramatically better CO tolerance and recoverability, coupling with the superior activity and durability for hydrogen evolution reaction (HER) in the alkaline electrolyte. This work demonstrates the significance of Rh in the alloy for improving the stability. This work offers a promising strategy for preparation of advanced trimetallic electrocatalysts for energy conversion applications.

3.
Biosens Bioelectron ; 148: 111823, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31671357

RESUMO

Nonmetallic plasmonic MoS2 nanosheets were synthesized by hydrothermal top-down method. MoS2 nanosheets had shown strong surface plasmon coupling (SPC) light absorption in the visible and near-infrared region. Herein, the nonmetallic plasmonic MoS2 nanosheets were employed to enhance the electrochemiluminescence (ECL) signal of sulfur doped boron nitrogen QDs (S-BN QDs) in this work. It is important to regulate the distance between ECL luminophore and plasmonic nanoparticles. On one hand, too closed distance can cause energy or electron transfer, which could quench the ECL intensity of nano-luminophore. On the other hand, plasmonic nanostructure cannot significantly affect the luminescence in the far distance. Therefore, we discussed the distance-dependent plasmon-enhanced ECL in detail with different length DNA chains. Furthermore, we constructed a hybridization chain reaction (HCR) amplification ECL sensing mode with the SPC-ECL strategy. The proposed DNA sensor can quantify hepatitis C virus (HCV) gene from 0.5 pmoL/L to 1 nmoL/L with a limit of detection (LOD) of 0.17 pmoL/L.

4.
J Hazard Mater ; 383: 121165, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31522067

RESUMO

As a recalcitrant fraction of petroleum, heavy hydrocarbons (including aromatics, resins, and asphaltenes) can remain in contaminated soils even after decades of weathering, thereby causing serious harm to the soil ecosystem and human health. Pyrolysis is a promising technique for remediating petroleum-contaminated soil. However, this technique still presents some drawbacks, such as high energy consumption and damage to soil properties. Therefore, an innovative method using hematite (Fe2O3) for the catalytic pyrolysis of weathered petroleum-contaminated soil was developed in this study. Compared with soil pyrolyzed without Fe2O3 at 400 °C for 30 min, the residual concentrations of aromatics, resins, and asphaltenes in soil pyrolyzed with 5.0% Fe2O3 were reduced by 67.8%, 52.3%, and 67.9%, respectively. After pyrolysis with 5.0% Fe2O3, the water-holding capacity of soil was considerably increased and the soil became darker and rougher. Scanning electron microscopy analysis showed that many small holes occurred on the surface of the pyrolytic soil. X-ray photoelectron spectrometer analysis showed that a thin layer of graphitic C was formed and deposited on the surface of the pyrolytic soil. We also observed that the wheat germination percentage and biomass yield in the soil pyrolyzed with 5.0% Fe2O3 were even higher than those in clean soil.

5.
J Nanosci Nanotechnol ; 20(5): 3287-3294, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635677

RESUMO

As the characterizations of electrode are meaningful for electric catalytic efficiency and mechanism, the improvement of electrode have raised considerable public concern in recent decades. However, the metal electrode have the drawbacks of high price and easy for toxicity, nano electrode restricted by difficulties for electrode coating, possibility of agglomeration, and abscission during reactions. Focus on those defects, the proposed study is going to establish a useful technique for polymer combined nano-electrode preparation. The morphology, functional groups, and other characterization of the Pt/TiO2/graphene particles and organic composite nano Pt/TiO2/graphene sheets were analyzed by transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), electrochemical impedance spectroscopy (EIS). To identify the stability of self-prepared electrodes, parameters such as catalysts dosage, current density and pH will be analyzed by using RBK5 as target pollutions. The results shows that after treatment for 50 min under optimized conditions (20 mA, 1 g/L NaCl), the degradation rate of acetaminophen almost reached 100%. After five times recycle, the self-prepared electrode could still maintained a high removal rate of 90%.

6.
J Chromatogr A ; : 460723, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31796249

RESUMO

Quantitative determination of endogenous compounds in biological samples has still been challenged by the absence of authentic blank matrix. Alternative strategy of surrogate matrix for preparing reference samples are prevalent due to its low cost and high availability. However, the evaluation system of surrogate matrix is not perfect. Herein, a novel multifunctional isotopic standards based steroidomics strategy was developed. Isotope-labeled standards were used not only as internal standards but also for the evaluation the feasibility of surrogate matrix, which improved the accuracy of assessment and could provide a new prospect for the quantitative analysis of endogenous compounds. Based on this approach, a detailed optimization from LC separation, MS detection to extraction conditions for global steroids in the steroidogenesis was firstly carried out. Characteristics and regularities of steroids in LC-MS were summarized to make references for further targeted or untargeted steroidomics study. Then eighteen steroids were quantified with high accuracy and high sensitivity in plasma from four types of cancer patients and healthy subjects using 1% BSA in PBS as surrogate matrix. And multi-steroids indexes with the best discriminating ability for lung, colorectal, breast and gastric cancer in different genders were identified successfully with Student's t-test, PLS-DA and logistic regression- ROC curve analysis. Finally, efficient cancer screening workflow was established by integrating the amine submetabolomics and lipidomics data of our previous studies. Taken together, the integrated steroidomics strategy could shed a light on the guidance for further steroidome as well as other endogenous compounds analysis and may provide a powerful tool for cancer diagnosis.

7.
Stem Cell Res ; 42: 101651, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31794942

RESUMO

Familial partial lipodystrophy type 2 (FPLD2) is a rare autosomal dominant metabolic disorder caused by heterozygous mutations in the LMNA gene, which encodes for the lamin A/C. A human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMCs) of a 30 year-old male patient with FPLD2 who had a heterozygous p.R349W (c.1045C > T) mutation in the LMNA gene using non-integrating episomal vector technique. This iPSC line offers a useful resource to investigate pathogenic mechanisms in FPLD2, as well as a cell-based model for drug development to treat FPLD2.

8.
Oncol Rep ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31789421

RESUMO

Emerging evidence has revealed that long noncoding RNAs (lncRNAs) play crucial roles in the development and progression of tumors. The present study aimed to examine the roles and illustrate the underlying mechanisms of lncRNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) in cervical cancer. The expression of lncRNA FTH1P3 and microRNA­145 (miRNA­145 or miR­145) in human cervical cancer samples and cervical cancer cell lines was detected by qRT­PCR (reverse transcription­quantitative polymerase chain reaction). FTH1P3 overexpression, siRNA plasmid, hsa­miR­145 mimic or hsa­miR­145 inhibitor were transfected. The target of FTH1P3 was predicted by bioinformatics analysis and validated by luciferase assay. Statistical significance analysis was performed by SPSS software. The results revealed that FTH1P3 was significantly upregulated in cervical cancer tissues compared with normal tissues. Increased expression of FTH1P3 was revealed in human cervical cancer cell lines compared with cervical normal epithelial cells. Downregulation of FTH1P3 inhibited cell proliferation, invasion and migration, and promoted apoptosis in cervical cancer cells. miR­145 was predicted and validated as a direct target of FTH1P3. Moreover, FTH1P3 siRNA partially attenuated the effects of the miR­145 inhibitor on cell viability and mobility in cervical cancer cells. The present results demonstrated that lncRNA FTH1P3 functioned as a promoting factor in cervical cancer by targeting miR­145.

9.
J Hypertens ; 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31790055

RESUMO

OBJECTIVE: The clinical utility of screening for pediatric metabolic syndrome (MetS) in children and adolescents is still controversial. We examined the performance of pediatric MetS vs. clustering of cardiovascular risk factors (which are the components of MetS) for predicting high carotid intima-media thickness (cIMT) in children and adolescents. METHODS: Participants included 2427 children and adolescents aged 6-17 years from population-based studies in three countries (Brazil, China and Italy). Pediatric MetS was defined using either the modified National Cholesterol Education Program Adult Treatment Panel III criteria or the modified International Diabetes Federation criteria. Clustering of cardiovascular risk factors was calculated as the sum of five components of MetS (i.e. central obesity, elevated blood pressure, elevated triglycerides, reduced HDL-cholesterol and elevated fasting blood glucose). High cIMT was defined as cIMT at least 95th percentile values for sex and age developed from European children. RESULTS: Presence of one, two or at least three cardiovascular risk factors (using the National Cholesterol Education Program Adult Treatment Panel III criteria), as compared with none, was associated with gradually increasing odds of high cIMT [odds ratios (95% confidence intervals): 1.60 (1.29-1.99), 2.89 (2.21-3.78) and 4.24 (2.81-6.39), respectively]. High cIMT was also associated with presence (vs. absence) of MetS (odds ratio = 2.88, 95% confidence interval = 1.95-4.26). However, clustering of cardiovascular risk factors predicted high cIMT markedly better than MetS (area under the curve of 0.66 vs. 0.54, respectively). Findings were similar using the International Diabetes Federation criteria for pediatric MetS. CONCLUSION: In children and adolescents, a graded score based on five cardiovascular risk factors (used to define MetS) predicted high cIMT markedly better than MetS. These findings do not support the clinical utility of MetS for screening youth at increased cardiovascular risk, as expressed in this study by high cIMT.

10.
Oncogene ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31801971

RESUMO

The original version of this Article contained an error in the author affiliations. Affiliation number 4 incorrectly read "Department of Gastroenterology and Hepatology, Tianjin Institute of Digestive Disease, Tianjin Institute of Digestive Disease". It should be "Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin 300052, China".

11.
Nucleic Acids Res ; 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31802121

RESUMO

Splice-switching antisense oligonucleotides (ASOs), which bind specific RNA-target sequences and modulate pre-mRNA splicing by sterically blocking the binding of splicing factors to the pre-mRNA, are a promising therapeutic modality to treat a range of genetic diseases. ASOs are typically 15-25 nt long and considered to be highly specific towards their intended target sequence, typically elements that control exon definition and/or splice-site recognition. However, whether or not splice-modulating ASOs also induce hybridization-dependent mis-splicing of unintended targets has not been systematically studied. Here, we tested the in vitro effects of splice-modulating ASOs on 108 potential off-targets predicted on the basis of sequence complementarity, and identified 17 mis-splicing events for one of the ASOs tested. Based on analysis of data from two overlapping ASO sequences, we conclude that off-target effects are difficult to predict, and the choice of ASO chemistry influences the extent of off-target activity. The off-target events caused by the uniformly modified ASOs tested in this study were significantly reduced with mixed-chemistry ASOs of the same sequence. Furthermore, using shorter ASOs, combining two ASOs, and delivering ASOs by free uptake also reduced off-target activity. Finally, ASOs with strategically placed mismatches can be used to reduce unwanted off-target splicing events.

12.
J Texture Stud ; 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804705

RESUMO

Objective evaluation methods for the elasticity, smoothness, and softness of cooked udon noodles were established on the basis of texture analysis. Noodles with different diameters and amylose contents were prepared to verify the reliability of the proposed evaluation methods. Results revealed that the elasticity of udon noodles could be examined by using the method that involved rinsing cooked noodles with 10°C cold water for 30 s and stretching a single strand of cooked noodle at 3 mm/s with an A/KIE probe until broken. The stiffness of the sample at 3.5 mm could be determined to indicate the elasticity caused by the size of dried udon noodles. The softness and smoothness of cooked udon noodles could be examined when cooled and drained for 30 s by using HDP/PFS probe. The elasticity of cooked udon noodles increased as their diameter increased, and their smoothness and softness significantly increased as the amylose content decreased (p < 0.05). Texture analysis could be used to quickly and accurately indicate the elasticity, softness, and smoothness of cooked udon noodles. The texture characteristics of udon noodles were related to their formulation, processing, and size. Establishing an adaptability evaluation method for the quality of udon noodles is a prerequisite for optimizing processing technologies and developing new products. Thus far, limited research has focused on objective methods for evaluating the texture of cooked udon noodles. This work developed valuable instrumental methods for examining the elasticity, softness, and smoothness of cooked udon noodles and provided noodle manufacturers a tool for selecting udon noodle formulations and processing technology based on texture analysis. This article is protected by copyright. All rights reserved.

13.
J Viral Hepat ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31755220

RESUMO

Antiviral therapy has been shown to improve the prognosis of hepatitis B virus (HBV) DNA-positive hepatocellular carcinoma (HCC) after radical treatment, but antiviral treatments require further optimization. This study aimed to evaluate the efficacies of different antiviral strategies with HCC patients after hepatectomy/ablation. This prospective, randomized, controlled, and multi-center trial enrolled HBV DNA-positive primary HCC patients after hepatectomy/ablation between January 2007 and January 2009. Patients were divided into four groups: early-combination (entecavir plus Peg-interferon [IFN]α-2a co-administration during year 1); late-combination (addition of Peg-IFNα-2a for 48 weeks after 1 year of entecavir); nucleos(t)ide analogue[NA] monotherapy; and non-antiviral treatment. Primary endpoints included recurrence-free survival and overall survival. A total of 447 patients were enrolled. The 2-year and 8-year recurrence free survival and 8-year overall survival rates were significantly higher in the early-combination group than in the other two antiviral groups (P<0.05). After 48 weeks treatment, more patients achieved an HBsAg reduction >1500 IU/ml and the mean HBsAg level was significantly lower in the early-combination group compared with the late-combination and NA monotherapy groups (P<0.05). Multivariate analysis showed that early-combination therapy and a reduction in HBsAg by >1500 IU/mL after 48 weeks of therapy correlated with reduced mortality and disease recurrence. Early introduction of combination antiviral treatment may represent a more effective therapeutic strategy for patients with HBV DNA-positive HCC after hepatectomy/ablation. A reduction in HBsAg by >1500 IU/mL after 48 weeks treatment is associated with reduced mortality and disease recurrence of HBV DNA-positive HCC patients after hepatectomy/ablation.

14.
Drug Deliv ; 26(1): 1068-1079, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31735094

RESUMO

Therapeutic application of unmodified camptothecin (CPT) is severely restricted by its extremely low water solubility and the instability of active lactone ring. In this study, a redox-sensitive CPT-OA conjugate containing the disulfide bond (CPT-SS-OA) was used to deliver the lactone-stabilized CPT for the improved antitumor efficacy. A non-sensitive CPT-OA was used as control to illuminate the role of disulfide bond. Both CPT-SS-OA and CPT-OA formulated in cremophor EL micelles (CM) displayed multiple therapeutic advantages: small diameter (∼14 nm), efficient cellular internalization, prolonged blood circulation, and favorable biodistribution. However, only CPT-SS-OA/CM achieved the superior chemotherapeutic efficacy over CPT solution in the Lewis lung carcinoma (LLC) cancer xenograft, which was ascribed to the accelerated release of the active lactone CPT responding to the elevated reductive glutathione in tumor cells. Such redox-sensitive lipophilic prodrugs represent an effective alternative strategy for the delivery of CPT in the active lactone form. This strategy can be used for other chemically unstable chemotherapeutant for the improved therapeutic efficacies.

15.
Mol Genet Genomics ; 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31735985

RESUMO

Pine resin, which typically consists of terpenoids, is a natural product used in various industrial applications. Oleoresin can be obtained from the xylem tissue by wounding the stem bark. Pinus massoniana (masson pine) is an important resin-tapping tree species that originated in southern China. Masson pines with different genetic backgrounds typically have different resin-yielding capacities (RYCs). However, the mechanisms underlying high resin yield in masson pines are unclear. The aim of this study was to identify the possible genetic regulation pathways and functional genes that influence the resin yield. In this study, we conducted transcriptomic and metabolomic studies of masson pine secondary xylem with high, medium, and low RYCs. A total of 230,068 unigenes and 3894 metabolites were identified from the tissue of the secondary xylem. Several differentially expressed regulation factors, including WRKY, bHLH, and ERF, and functional genes such as PKc and LRR-RLKs, were identified among these masson pines. The Kyoto Encyclopedia of Genes and Genomes pathways were mainly focused on diterpenoid biosynthesis, plant hormone signal transduction, and ABC transporters. Furthermore, integration of the transcriptomic and metabolomic data indicated that the PKc- and LRR-RLK-related regulatory and metabolic pathways may play critical roles in the biosynthesis of terpenoids. These above results improve our understanding of the biosynthesis mechanism of oleoresin in P. massoniana and facilitate further research work into the functional analysis of these candidate genes.

16.
J Dig Dis ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31756284

RESUMO

AIM: This study aimed to investigate the effectiveness and safety of transparent cap-assisted endoscopy in esophageal foreign body removal. METHODS: Patients with esophageal foreign body ingestion had cap-assisted or conventional endoscopy between October 2004 and July 2018. Propensity score matching was performed. The success rate of the procedure, procedure time, clearness of the endoscopic view and adverse event rates were compared between the two groups. RESULTS: Of the 838 patients with confirmed foreign body ingestion, 728 (86.9%) underwent endoscopic intervention. After matching, there were 224 pairs of patients. There was no difference between transparent cap-assisted endoscopy and conventional endoscopy regarding the success rate of the procedure (100% vs 99.1%, respectively). Transparent cap-assisted endoscopy was associated with shorter procedure times for removing jujube pits, fish bones and other sharp objects (4.24 ± 2.81 minutes vs 7.62 ± 8.15 minutes, P = 0.001; 2.99 ± 2.15 minutes vs 6.49 ± 6.54 minutes, P < 0.001; 4.29 ± 3.36 minutes vs 10.60 ± 19.79 minutes, P = 0.027, respectively) and higher ratios for clear endoscopic view in the extraction of jujube pits, fish bones, poultry bones and other sharp objects (98% vs 43.4%, 97.5% vs 74.1%, 100% vs 81.3%, and 100% vs 82.7%; P < 0.001, P = 0.014, P = 0.049, and P = 0.026, respectively). No significant differences in the adverse event rates were observed between the two groups (P = 1.000). CONCLUSIONS: The transparent cap-assisted technique is considered an effective and safe method to remove sharp esophageal foreign bodies. This article is protected by copyright. All rights reserved.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31756352

RESUMO

BACKGROUND: Keratinocytes can function as innate immune cells under oxidative stress and aggravate cutaneous T cell response that undermines melanocytes in vitiligo. The NLR family pyrin domain containing 3 (NLRP3) inflammasome is a regulator of innate immunity that exists in keratinocytes. However, the role of NLRP3 inflammasome in the pathogenesis of vitiligo has not been investigated. OBJECTIVE: We sought to explicate the contribution of activated NLRP3 inflammasome in keratinocytes to the autoimmune response in vitiligo. METHODS: Perilesional and serum samples from vitiligo patients were collected to examine the status of NLRP3 inflammasome in vitiligo. Cultured keratinocytes were treated with H2O2 to investigate the mechanism for NLRP3 inflammasome activation under oxidative stress. Peripheral blood T cells were extracted from vitiligo patients to explore the influence of NLRP3 inflammasome on T cell response in vitiligo. RESULTS: The expressions of NLRP3 and downstream cytokine IL-1ß were consistently elevated in perilesional keratinocytes of vitiligo. Notably, serum IL-1ß level was increased in vitiligo patients, correlated with disease activity and severity and decreased after effective therapy. Furthermore, oxidative stress promoted NLRP3 inflammasome activation in keratinocytes via transient receptor potential cation channel subfamily M member 2 (TRPM2), a redox-sensitive cation channel, which was dependent on TRPM2-mediated calcium influx. More importantly, blocking TRPM2-induced NLRP3 inflammasome activation in keratinocytes impaired the chemotaxis for CD8+ T cells and inhibited the production of cytokines in T cells in vitiligo. CONCLUSION: Oxidative stress-induced NLRP3 inflammasome activation in keratinocytes promotes cutaneous T cell response, which could be targeted for the treatment of vitiligo.

18.
Aging (Albany NY) ; 11(21): 9556-9568, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31682592

RESUMO

Dexmedetomidine is known to alleviate cerebral ischemia-reperfusion injury (CIRI). We established a rat model of CIRI, which exhibited higher neurological deficit scores and a greater number of apoptotic cells in the cerebral ischemic penumbra than controls. Dexmedetomidine reversed the neuronal apoptosis and improved neurological function in this model. We then examined Sigma-1 receptor (Sig-1R) expression on the endoplasmic reticulum (ER) in brain tissues at different reperfusion time points. Sig-1R expression increased with CIRI and decreased with increasing reperfusion times. After 24 hours of reperfusion, dexmedetomidine upregulated Sig-1R expression, and ER stress proteins (GRP78, CHOP, JNK and Caspase-3) were detected in brain tissues with Western blotting. Moreover, GRP78 expression followed a pattern similar to Sig-1R. Dexmedetomidine induced GRP78 expression but inhibited CHOP, Caspase-3 and phosphorylated-JNK expression in brain tissues. A Sig-1R-specific inhibitor reduced GRP78 expression and partially inhibited the upregulation of GRP78 by dexmedetomidine. The inhibitor also increased CHOP and Caspase-3 expression and partially reversed the inhibitory effects of dexmedetomidine on these pro-apoptotic ER stress proteins. These results suggest that dexmedetomidine at least partially inhibits ER stress-induced apoptosis by activating Sig-1R, thereby attenuating brain damage after 24 hours of ischemia-reperfusion.

19.
J Integr Plant Biol ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31755194

RESUMO

Oscillations in cytosolic free calcium determine the polarity of tip-growing root hairs. The Ca2+ channel cyclic nucleotide gated channel 14 (CNGC14) contributes to the dynamic changes in Ca2+ concentration gradient at the root hair tip. However, the mechanisms that regulate CNGC14 are unknown. In this study, we detected a direct interaction between calmodulin 7 (CaM7) and CNGC14 through yeast two-hybrid and bimolecular fluorescence complementation assays. We demonstrated that the third EF-hand domain of CaM7 specifically interacts with the cytosolic C-terminal domain of CNGC14. A two-electrode voltage clamp assay showed that CaM7 completely inhibits CNGC14-mediated Ca2+ influx, suggesting that CaM7 negatively regulates CNGC14-mediated calcium signaling. Furthermore, CaM7 overexpressing lines phenocopy the short root hair phenotype of a cngc14 mutant and this phenotype is insensitive to changes in external Ca2+ concentrations. We, thus, identified CaM7-CNGC14 as a novel interacting module that regulates polar growth in root hairs by controlling the tip-focused Ca2+ signal. This article is protected by copyright. All rights reserved.

20.
Transl Oncol ; 13(1): 42-56, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31760268

RESUMO

Colon cancer is the third most commonly diagnosed cancer in the United States. Recent reports have shown that the location of the primary tumor is of clinical importance. Patients with right-sided colon cancers (RCCs) (tumors arising between the cecum and proximal transverse colon) have poorer clinical outcomes than those with left-sided colon cancers (LCCs) (tumors arising between the distal transverse colon and sigmoid colon, excluding the rectum). Interestingly, women have a lower incidence of colon cancer than men, but have a higher propensity for RCC. The reason for this difference is not known; however, identification of sex-specific differences in gene expression by tumor anatomical location in the colon could provide further insight. Moreover, it could reveal important predictive markers for response to various treatments. This study provides a comprehensive bioinformatic analysis of various genes and molecular pathways that correlated with sex and anatomical location of colon cancers using four publicly available annotated data sets housed in the National Center for Biotechnology Information's Gene Expression Omnibus. We identified differentially expressed genes in tumor tissues from women with RCC, which showed attenuated energy and nutrient metabolism when compared with women with LCC. Specifically, we showed the downregulation of 5' AMP-activated protein kinase alpha subunit (AMPKα) and anti-tumor immune responses in women with RCC. This difference was not seen when comparing tumor tissues from men with RCC to men with LCC. Therefore, women with RCC may have a specific metabolic and immune phenotype which accounts for differences in prognosis and treatment response.

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