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1.
Hepatol Int ; 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36083440

RESUMO

BACKGROUND: The efficacy of nucleos(t)ide analogs (NAs) in non-cirrhotic chronic hepatitis B (CHB) patients is well-established. However, their impact on complications of portal hypertension in advanced chronic liver disease (ACLD) is less well characterized. METHODS: MEDLINE/PubMed, EMBASE, Web of Science, Cochrane Central Register of Controlled Trials, and abstracts of major international hepatology meetings were searched for publications from Jan 1, 1995 to Nov 30, 2021. Randomized control trials and observational studies reporting the efficacy of NAs in ACLD patients were eligible. Pooled risk ratios (RRs) for outcomes of interest were calculated with a random-effect or fixed-effect model, as appropriate. RESULTS: Thirty-nine studies including 14,212 ACLD patients were included. NA treatment was associated with reduced risks of overall hepatic decompensation events (RR, 0.51; 95% confidence interval [CI]: 0.37-0.71), such as variceal bleeding (RR, 0.44; 95% CI: 0.26-0.74) and ascites (RR, 0.10; 95% CI: 0.01-1.59), on a trend-wise level. Moreover, the risks of hepatocellular carcinoma (HCC) (RR, 0.48; 95% CI: 0.30-0.75) and liver transplantation/death (RR, 0.36; 95% CI: 0.25-0.53) were also reduced by NA treatment and the first-line NAs were superior to non-first-line NAs in improving these outcomes (RR, 0.85; 95% CI: 0.75-0.97 and RR, 0.85; 95% CI: 0.73-0.99, respectively). CONCLUSION: NA therapy lowers the risk of portal hypertension-related complications, including variceal bleeding, HCC, and liver transplantation/death.

2.
Nat Commun ; 13(1): 5201, 2022 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-36057726

RESUMO

Supramolecular self-assembly of µm-to-mm sized components is essential to construct complex supramolecular systems. However, the selective assembly to form designated structures at this length scale is challenging because the short-ranged molecular recognition could hardly direct the assembly of macroscopic components. Here we demonstrate a self-sorting mechanism to automatically identify the surface chemistry of µm-to-mm components (A: polycations; B: polyanions) based on the A-B attraction and the A-A repulsion, which is realized by the additivity and the competence between long-ranged magnetic/capillary forces, respectively. Mechanistic studies of the correlation between the magnetic/capillary forces and the interactive distance have revealed the energy landscape of each assembly pattern to support the self-sorting results. By applying this mechanism, the assembly yield of ABA trimers has been increased from 30%~40% under conventional conditions to 100% with self-sorting. Moreover, we have demonstrated rapid and spontaneous self-assembly of advanced chain-like structures with alternate surface chemistry.


Assuntos
Fenômenos Magnéticos , Fenômenos Físicos
3.
J Orthop Surg Res ; 17(1): 409, 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36064584

RESUMO

BACKGROUND: Malunion and nonunion of vertically displaced pelvic fractures result in lower limb length discrepancies, claudication, and pain. There have been few previous reports of this type of corrective surgery for these old pelvic fractures. We present a surgical technique of sacral osteotomy combined with triangular osteosynthesis in the treatment of malunion and nonunion of vertically displaced pelvic fractures and report on its short-term clinical results. METHODS: We retrospectively reviewed nine patients (five males and four females) with malunion or nonunion of vertically displaced pelvic fractures treated with sacral osteotomy and triangular osteosynthesis from April 2015 to January 2020. The age ranged from 14 to 45 years (average, 30.7 years). The time from injury to deformity correction surgery ranged from 3 months to 5 years (average, 12.8 months). The vertical displacement of a unilateral hemipelvis was 3.0-4.5 cm (average, 3.80 cm). According to AO/OTA classification at the initial fracture, there are eight cases in type C1.3 and one case in type C3.3. Sacral osteotomy and triangular osteosynthesis were used in all nine patients. The degree of unilateral hemipelvic reduction was assessed postoperatively based on measurements from the anteroposterior (AP) X-ray. Majeed score and pain visual analog scale (VAS) were used to assess the therapeutic effect of the patients during follow-up. RESULTS: In all nine patients, postoperative AP X-ray showed correction displacement of 1.7-3.9 cm (average, 3.20 cm). All the patients were followed up for 6-36 months (average, 12.7 months). At the last follow-up, the Majeed score of pelvic fracture increased from an average of 53.9 points (30-84 points) preoperatively to 87.0 points (72-94 points), and the VAS score for pain decreased from an average of 6.0 points (4-8 points) preoperatively to 1.2 points (0-3 points). None had complications like infection, implant broken, screw loosening, iatrogenic nerve, and blood vessel injury. CONCLUSION: Sacral osteotomy combined with triangular osteosynthesis for the treatment of pelvic malunion and nonunion caused by sacral fractures can correct significantly vertical displacement of a unilateral pelvis, prolong limb length, and reconstruct the stability of a pelvic ring, achieving good clinical results.


Assuntos
Fraturas Ósseas , Ossos Pélvicos , Adolescente , Adulto , Feminino , Fraturas Ósseas/diagnóstico por imagem , Fraturas Ósseas/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Osteotomia , Dor , Ossos Pélvicos/diagnóstico por imagem , Ossos Pélvicos/lesões , Ossos Pélvicos/cirurgia , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
4.
Contrast Media Mol Imaging ; 2022: 7998725, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072626

RESUMO

In order to investigate the clinical efficacy of acupuncture combined with traditional Chinese medicine fumigation in the treatment of rheumatoid arthritis (RA) by meta-analysis, literature related to the treatment of rheumatoid arthritis by acupuncture combined with traditional Chinese medicine fumigation are searched in line with the research direction. Meta-analysis is conducted by RevMan 5.2 software. A total of 17 literature are included and the publication bias of the included literature is low. The experimental results show that acupuncture combined with traditional Chinese medicine fumigation can reduce the inflammatory response and clinical symptoms of RA patients, and the addition of acupuncture can improve the clinical efficacy, which has a certain feasibility of promotion.


Assuntos
Terapia por Acupuntura , Artrite Reumatoide , Terapia por Acupuntura/métodos , Artrite Reumatoide/terapia , Fumigação , Humanos , Medicina Tradicional Chinesa , Resultado do Tratamento
5.
Med Sci Monit ; 28: e937572, 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36059134

RESUMO

BACKGROUND Cognitive decline has become a public health concern as it causes adverse economic and socio-psychological outcomes. Studies have assessed the effects of history of stroke or high-sugar diet on cognitive decline of older adults, but whether there was interaction between high-sugar diet and history of stroke in affecting cognitive decline was still unclear. The present study explored the interaction between high-sugar diet and history of stroke in affecting the risk of cognitive decline in older adults. MATERIAL AND METHODS This cross-sectional study included 2710 subjects. Univariate analysis was applied to explore the associations of high-sugar diet or history of stroke in affecting cognitive decline. The relative excess risk of interaction (RERI), synergy index (SI), and attributable proportion of interaction (API) were used to assess the interaction of high-sugar diet and history of stroke in risk of cognitive decline. RESULTS An increased risk of cognitive decline was observed in people with history of stroke [odds ratio (OR)=1.826, 95% confidence interval (CI): 1.124-2.968] and high-sugar diet (OR=1.509, 95% CI: 1.109-2.052). The confidence intervals of interaction indicators RERI=1.257 (95% CI: 0.188-2.326) and API=0.386 (95% CI: 0.135-0.638) did not contain 0 and were both >0; the confidence interval of SI=2.261 (95% CI: 1.063-4.809) did not contain 1 and were >1. CONCLUSIONS A high-sugar diet and history of stroke interacted synergically in association with cognitive decline in older adults, which might provide a reference for management of cognition in older adults.


Assuntos
Disfunção Cognitiva , Acidente Vascular Cerebral , Idoso , Disfunção Cognitiva/complicações , Estudos Transversais , Dieta/efeitos adversos , Humanos , Acidente Vascular Cerebral/complicações , Açúcares
6.
Occup Ther Int ; 2022: 8274445, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36051091

RESUMO

It is aimed at studying the role of parenting style on the career planning of vocational students in the context of information technology. This design takes the first-year and second-year students of two higher vocational colleges as the survey objects. From the perspective of educational psychology, firstly, the parenting style scale of these students is constructed, and meanwhile, the dimension design of career planning is carried out. Secondly, through the questionnaire survey, the career planning of students and the status quo of parenting styles are investigated, and the sample data are statistically analyzed by data analysis methods such as standard deviation analysis, cluster analysis, and correlation analysis. Finally, hypotheses are formulated and justified. The results reveal that the total score for career planning and the average score of each dimension are more than 3 points, indicating that vocational students have certain plans for their future careers. There are great differences in the gender of parenting styles, and the parental acceptance/involvement (t = 3.389, p < 0.01) dimension of the parenting style of the first-year students is greatly higher than that of the second-year students. On the dimension of psychological autonomy (t = -2.066, p < 0.05), the score of second-year students is distinctly higher than that of the first year. There are obvious differences in grades from the dimensions of parental acceptance/participation and psychological autonomy. There is no observable difference in whether it is an only child (p > 0.05) and the place of origin (p > 0.05). In terms of the total score and each dimension of career planning, the authoritative type scored the highest, and the neglect type scored the lowest. Parents' participation and acceptance behavior of vocational students are markedly related to their career planning. Parental punishment and acceptance are distinctly positively correlated with the career planning of these students and their two dimensions. Psychological autonomy is memorably associated with the domain of exploration.


Assuntos
Terapia Ocupacional , Poder Familiar , Criança , Humanos , Tecnologia da Informação , Relações Pais-Filho , Poder Familiar/psicologia , Psicologia Educacional , Estudantes/psicologia , Inquéritos e Questionários
7.
Artigo em Inglês | MEDLINE | ID: mdl-36048974

RESUMO

Many online services allow users to participate in various group activities such as online meeting or group buying, and thus need to provide user groups with services that they are interested. The group recommender systems (GRSs) emerge as required and provide personalized services for various online user groups. Data sparsity is an important issue in GRSs, since even fewer group-item interactions are observed. Moreover, the group and the group members have complex and mutual relationships with each other, which exacerbates the difficulty in modeling the preferences of both a group and its members for recommendation. The cross-domain recommender system (CDRS) is a solution to alleviate data sparsity and assist preference modeling by transferring knowledge from a source domain which has relatively dense data to another. The existing CDRSs are usually developed for individual users and cannot be directly applied for group recommendation. To alleviate the data sparsity issue in GRSs, we first study the cross-domain group recommendation problem and propose a hierarchical attention network-based cross-domain group recommendation method, called HAN-CDGR. HAN-CDGR takes the advantage of data from a source domain to benefit recommendation generation for both the individual users and groups in the target domain which has data sparsity and cannot generate accurate recommendation. In HAN-CDGR, a hierarchical attention network is constructed to learn and model individual and group preferences, with consideration of both group members' interactions and dynamic weights and the complex relationships between individuals and groups. Adversarial learning is used to effectively transfer knowledge from a source domain to the target domain. Extensive experiments, which demonstrate the effectiveness and superiority of our proposal, providing accurate recommendation for both individual users and groups, are conducted on three tasks.

8.
Nanoscale ; 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36052726

RESUMO

The rapid emergence of pathogenic bacteria poses a serious threat to global health. Notably, traditional antibiotic therapies suffer from the risk of strengthening bacterial drug resistance. Sonodynamic therapy (SDT) combining sonosensitizers and low-intensity ultrasound (US) has broadened the way towards treating drug-resistant bacteria. The allure of this therapy emerges from the capacity to focus the US energy on bacterial infection sites buried deep in tissues, locally activating the sonosensitizers to produce cytotoxic reactive oxygen species (ROS) with the ability to induce bacterial death. The past decade has witnessed the rapid development of antibacterial SDT owing to their excellent penetration, favorable biocompatibility and specific targeting ability. This review summarizes available sonosensitizers for antibacterial SDT, and digs into innovative biotechnologies to improve SDT efficiency, such as enhancing the targeting ability of sonosensitizers, image-guided assisted SDT, improvement of hypoxia and combination of SDT with other therapies. Finally, we conclude with the present challenges and provide insights into the future research of antibacterial SDT.

9.
Hum Cell ; 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36053455

RESUMO

Recent studies have demonstrated that several long non-coding RNAs (lncRNAs) play an important role in the occurrence and development of osteosarcoma (OS). However, more lncRNAs and their mechanisms in regulating growth and migration of OS cells remain to be investigated. In this study, we identified an lncRNA called DUXAP10 by analysis of GEO data, which was significantly up-regulated in OS tissues and cell lines. Experiments in vitro revealed that lncRNA DUXAP10 promoted proliferation, migration, and invasion of OS cells and inhibited their apoptosis. We also demonstrated that DUXAP10 promoted the formation and growth of OS by tumor formation assay. Furthermore, SOX18 was identified as a critical downstream target of DUXAP10 by transcriptome RNA-seq. Mechanistically, DUXAP10 mainly localized in cytoplasm and could specifically bind to HuR to increase the stability of SOX18 mRNA. Meanwhile, SOX18 knockdown largely reversed increased proliferation of OS cells induced by DUXAP10 overexpression. Findings from this study indicate that lncRNA DUXAP10 can act as an oncogene in osteosarcoma by binding HuR to up-regulate the expression of SOX18 at a post-transcriptional level, which may provide a new target for OS clinical diagnosis and treatment.

10.
Cancer Cell ; 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36084651

RESUMO

How glucose metabolism remodels pro-tumor functions of tumor-associated macrophages (TAMs) needs further investigation. Here we show that M2-like TAMs bear the highest individual capacity to take up intratumoral glucose. Their increased glucose uptake fuels hexosamine biosynthetic pathway-dependent O-GlcNAcylation to promote cancer metastasis and chemoresistance. Glucose metabolism promotes O-GlcNAcylation of the lysosome-encapsulated protease Cathepsin B at serine 210, mediated by lysosome-localized O-GlcNAc transferase (OGT), elevating mature Cathepsin B in macrophages and its secretion in the tumor microenvironment (TME). Loss of OGT in macrophages reduces O-GlcNAcylation and mature Cathepsin B in the TME and disrupts cancer metastasis and chemoresistance. Human TAMs with high OGT are positively correlated with Cathepsin B expression, and both levels predict chemotherapy response and prognosis of individuals with cancer. Our study reports the biological and potential clinical significance of glucose metabolism in tumor-promoting TAMs and reveals insights into the underlying mechanisms.

11.
Appl Environ Microbiol ; : e0121222, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36073939

RESUMO

In industrial production, the precursor of l-ascorbic acid (L-AA, also referred to as vitamin C), 2-keto-l-gulonic acid (2-KLG), is mainly produced using a classic two-step fermentation process performed by Gluconobacter oxydans, Bacillus megaterium, and Ketogulonicigenium vulgare. In the second step of the two-step fermentation process, the microbial consortium of K. vulgare and B. megaterium is used to achieve 2-KLG production. K. vulgare can transform l-sorbose to 2-KLG, but the yield of 2-KLG is much lower in the monoculture than in the coculture fermentation system. The relationship between the two strains is too diverse to analyze and has been a hot topic in the field of vitamin C fermentation. With the development of omics technology, the relationships between the two strains are well explained; nevertheless, the cell-cell communication is unclear. In this review, based on current omics results, the interactions between the two strains are summarized, and the potential cell-cell communications between the two strains are discussed, which will shed a light on the further understanding of synthetic consortia.

12.
Microsyst Nanoeng ; 8: 99, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119378

RESUMO

Flexible human-machine interfaces show broad prospects for next-generation flexible or wearable electronics compared with their currently available bulky and rigid counterparts. However, compared to their rigid counterparts, most reported flexible devices (e.g., flexible loudspeakers and microphones) show inferior performance, mainly due to the nature of their flexibility. Therefore, it is of great significance to improve their performance by developing and optimizing new materials, structures and design methodologies. In this paper, a flexible acoustic platform based on a zinc oxide (ZnO) thin film on an aluminum foil substrate is developed and optimized; this platform can be applied as a loudspeaker, a microphone, or an ambient sensor depending on the selection of its excitation frequencies. When used as a speaker, the proposed structure shows a high sound pressure level (SPL) of ~90 dB (with a standard deviation of ~3.6 dB), a low total harmonic distortion of ~1.41%, and a uniform directivity (with a standard deviation of ~4 dB). Its normalized SPL is higher than those of similar devices reported in the recent literature. When used as a microphone, the proposed device shows a precision of 98% for speech recognition, and the measured audio signals show a strong similarity to the original audio signals, demonstrating its equivalent performance compared to a rigid commercial microphone. As a flexible sensor, this device shows a high temperature coefficient of frequency of -289 ppm/K and good performance for respiratory monitoring.

13.
Am J Cancer Res ; 12(8): 3662-3678, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119836

RESUMO

Recent advances in cancer research have revealed a close relationship between mitochondrial dysfunction and cancer development. Human COX assembly factor 3 (COA3), also known as CCDC56, is a mitochondrial transmembrane protein responsible for cytochrome c oxidase (COX) protein complex assembly. However, the clinical implication and biological functions of COA3 remain unexplored in human cancers, including non-small cell lung cancer (NSCLC). Here, we found that COA3 is overexpressed at both mRNA and protein levels in human NSCLC cells, mainly as a result of decreased miR-338-3p level. The protein expression level of COA3 is positively associated with lymph node metastasis and predicts poor survival in patients with NSCLC. Silencing of COA3 significantly attenuated, while forced COA3 expression enhanced the migration and invasiveness of NSCLC cells. Mechanistically, we found that aerobic glycolysis, induced at least in part by dynamic-related protein 1 (DRP1) phosphorylation-mediated mitochondrial fragmentation, contributed to COA3-promoted NSCLC metastasis. Together, our study illustrates that COA3 plays a crucial role in NSCLC carcinogenesis, implying COA3 as a prognostic marker and treatment target in NSCLC.

14.
Virol J ; 19(1): 150, 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36115975

RESUMO

BACKGROUND: Integrating CRISPR-Cas12a sensors with isothermal signal amplification can be exploited to develop low-cost, disposable, and ultrasensitive assays for the diagnostics of human pathogens. METHODS: RT-RAA-Cas12a-mediated real-time or end-point fluorescent and lateral flow strip (LFS) assays for direct detection of norovirus (NOV) genotype GII.4 or GII.17 were explored. RESULTS: The results showed that our RT-RAA-Cas12a-mediated fluorescent and LFS assay could detect NOV GII.4 or GII.17 by targeting the viral protein 1 gene. Our RT-RAA-Cas12a-mediated fluorescent and LFS assay can specifically detect NOV GII.4 or GII.17 with no cross-reactivity for other related viruses. The low limit of detection could reach 0.1 copies/µL within approximately 30-40 min, and the results were visualized using an ultraviolet light illuminator or on a LFS without complex equipment. In addition, our RT-RAA-Cas12a-mediated fluorescent and LFS assay provided a visual and faster alternative to real-time RT-PCR assay, with 95.7% and 94.3% positive predictive agreement and 100% negative predictive agreement. CONCLUSIONS: Together, our RT-RAA-Cas12a-mediated approach would have a great potential for point-of-care diagnostics of NOV GII.4 and/or GII.17 in resource-limited settings.


Assuntos
Infecções por Caliciviridae , Norovirus , Sistemas CRISPR-Cas , Infecções por Caliciviridae/diagnóstico , Genótipo , Humanos , Norovirus/genética , Sensibilidade e Especificidade , Proteínas Virais/genética
15.
iScience ; 25(10): 105057, 2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36124233

RESUMO

In this report, we provide evidence showing diminished expression of the mineral dust-induced gene (mdig), a previously identified oncogenic gene, in human triple negative breast cancer (TNBC). Using a mouse model of orthotopic xenograft of the TNBC MDA-MB-231 cells, we demonstrate that mdig promotes the growth of primary tumors but inhibits metastasis of these cells in vivo. Knockout of mdig resulted in an enhancement of H3K36me3 in the genome and upregulation of some X chromosome-linked genes for cell motility, invasion, and metastasis. Silencing MAGED2, one of the most upregulated and H3K36me3-enriched genes resulted from mdig depletion, can partially reverse the invasive migration of the mdig knockout cells. The anti-metastatic and inhibitory role of mdig on H3K36me3 was cross-validated in another cell line, A549 lung cancer cells. Together, our data suggest that mdig is antagonist against H3K36me3 that enforces expression of genes, such as MAGED2, for cell invasion and metastasis.

16.
Artigo em Inglês | MEDLINE | ID: mdl-36066802

RESUMO

Sewage irrigation has solved the shortage of agricultural water and increased the content of heavy metal(loid)s (HMs) in soil-crop systems, which harms human health via the food chain. In this study, 43 pairs of soil and corn samples (leaf, stem1, stem2, stem3, root, husk, grain, and corncob) were collected in the Dongdagou (DDG) and Xidagou (XDG) streams of Baiyin City. Fraction and transfer of As and Hg were investigated, and toxicological effects and contamination were assessed in soil-corn systems. The results showed that the mean values of As and Hg in soil were 33.79 mg/kg and 0.96 mg/kg, respectively, which exceeded the soil background values in Gansu Province. As and Hg are mainly dominated by the residual fraction. Total and bioavailability contributed significantly to As and Hg accumulation in corn, with root, stem3, and leaf accumulating more strongly. The results based on the bioavailability concentration soil-corn transfer factor indicated that As and Hg tended to accumulate more in the root, stem3, and leaf and less in grain, and further assessment of the human health effects of consuming contaminated cron is needed. Scanning electron microscope (SEM) and Fourier transform infrared (FTIR) results showed that As and Hg were not significantly toxic to corn parts, indicating morphology. As and Hg were bound to hydroxyl groups in the outer epidermal cell wall of the roots, thereby reducing upward translocation. The trinity assessment (TA) model results indicated that the most severe contamination was found in root and stem1. The TA provides a practical tool for soil-cron systems and helps develop management strategies to prevent ecological hazards.

17.
Inflamm Res ; 2022 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-36057911

RESUMO

OBJECTIVES: This study aimed to identify the role of ferroptosis in intracranial aneurysm (IA). METHODS: GSE122897, GSE75436, GSE15629, and GSE75434 datasets were downloaded from the Gene Expression Omnibus database. The differentially expressed ferroptosis-related genes (DEFRGs) were selected to construct a diagnostic model integrating with machine learning. Then, a consensus clustering algorithm was performed to classify IA patients into distinct ferroptosis-related clusters. Functional analyses, including GO, KEGG, GSVA, and GSEA analyses, were conducted to elucidate the underlying mechanisms. ssGSEA and xCell algorithms were performed to uncover the immune characteristics. RESULTS: We identified 28 DEFRGs between IAs and controls from the GSE122897 dataset. GO and KEGG results showed that these genes were enriched in cytokine activity, ferroptosis, and the IL-17 signaling pathway. Immune analysis showed that the IAs had higher levels of immune infiltration. A four FRGs model (MT3, CDKN1A, ZEP69B, and ABCC1) was established and validated with great IA diagnostic ability. We divided the IA samples into two clusters and found that cluster 2 had a higher proportion of rupture and immune infiltration. We identified 10 ferroptosis phenotypes-related markers in IAs. CONCLUSION: Ferroptosis and the immune microenvironment are closely associated with IAs, providing a basis for understanding the IA development.

18.
J Hepatocell Carcinoma ; 9: 885-900, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36061235

RESUMO

Background: Helicases have been classified as a class of enzymes that determine the stability of the cellular genome. There is growing evidence that helicases can help tumor cells resist drug killing by repairing Deoxyribose Nucleic Acid (DNA) or stabilizing transcription, which may contribute to the understanding of drug resistance. Currently, identifying cancer biomarkers among helicases and stratifying patients according to helicase activity will be able to guide treatment well. Methods: We clustered 371 hepatocellular carcinoma (HCC) patients from The Cancer Genome Atlas (TCGA) by consensus clustering based on helicase expression profiles to identify potential molecular subtypes. The Multiscale Embedded Gene Co-Expression Network Analysis (MEGENA) algorithm was used to find core differential gene modules between different molecular subtypes, and single-cell analysis was utlized to explore the potential function of hub gene. Immunohistochemical (IHC) staining was used to verify the diagnostic value of DDX56 and its ability to reflect the proliferation efficiency of cancer cells. Results: We identified two subtypes associated with helicase. High helicase subtype was associated with poor clinical outcome, massive M0 macrophage infiltration, and highly active cell proliferation features. In addition, we identified a new biomarker, DDX56, which has not been reported in HCC, was highly expressed in HCC tissues, associated with poor prognosis, and was also shown to be associated with high cell proliferative activity. Conclusion: In conclusion, based on helicase expression profiles, we have developed a new classification system for HCC, which is a proliferation-related system, and has clinical significance in evaluating prognosis and treating HCC patients, including immunotherapy and chemotherapy. In addition, we identified a new biomarker, DDX 56, which is overexpressed in HCC tissues, predicts a poor prognosis and is a validated index of tumor cell proliferation.

19.
Plant J ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069460

RESUMO

Vacuolar H+ -ATPase (V-ATPase) has diverse functions related to plant development and growth. It creates the turgor pressure that drives cell growth by generating the energy needed for the active transport of solutes across the tonoplast. Additionally, V-ATPase is a large protein complex made up of multiheteromeric subunits, some of which have unknown functions. In this study, a forward genetics-based strategy was employed to identify the vab3 mutant, which displayed resistance to isoxaben, a cellulose synthase inhibitor that could induce excessive transverse cell expansion. Map-based cloning and genetic complementary assays demonstrated that V-ATPase B subunit 3 (VAB3) was associated with the observed insensitivity of the mutant to isoxaben. Analysis of the vab3 mutant revealed defective ionic homeostasis and hypersensitivity to salt stress. Treatment with a V-ATPase inhibitor exacerbated ionic tolerance and cell elongation defects in vab3 mutant. Notably, exogenous low-dose Ca2+ or Na+ could partially restore the isoxben resistance of vab3 mutant, suggesting a relationship between VAB3-regulated cell growth and ion homeostasis. Taken together, the results of this study suggest that the V-ATPase subunit VAB3 is required for cell growth and ion homeostasis in Arabidopsis.

20.
Arch Psychiatr Nurs ; 40: 68-76, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36064248

RESUMO

AIM: To analyze and integrate the incidence and its influencing factors on workplace violence among psychiatric nurses in China. BACKGROUND: Despite the fact that an increasing number of studies in China and elsewhere have focused on workplace violence among psychiatric nurses, there is presently no research to thoroughly explain the determining variables of violence faced by psychiatric nurses. DESIGN: A systematic review and meta-analysis was conducted. METHOD: PubMed, Embase, Cohrane Library, CNKI database, Wanfang database, and VIP database were used to search for English-language literature. The search deadline is June 15, 2021. The quality of the included literature was assessed, data was retrieved, and the meta-analysis was performed using Stata 16.0 software. RESULTS: A total of 19 works of literature, including 5926 patients, were included. Meta-analysis results showed that the incidence of workplace violence was about 78%[ES = 0.78, 95%CI(0.65,0.88)]. The possible factors for Chinese psychiatric nurses suffering from violence include gender, education, working years, whether they are an only child, age, height, working hours, and the form of employment. CONCLUSIONS: The incidence of workplace violence incidence among psychiatric nurses in China is high, and managers should employ individualized intervention methods based on the variables that influence it. IMPLICATIONS FOR NURSING MANAGEMENT: Managers should attach great importance to the occurrence of workplace violence. In training to prevent workplace violence, we should pay attention to the disparities in age and working abilities of nurses. Nurses should be trained in recognizing violent conduct, early warning indications, communication skills, and emergency response tactics. Managers should be able to schedule shifts in a flexible manner. Various techniques to enhance the nurse's working environment.


Assuntos
Recursos Humanos de Enfermagem no Hospital , Enfermagem Psiquiátrica , Violência no Trabalho , China/epidemiologia , Humanos , Incidência , Recursos Humanos de Enfermagem no Hospital/psicologia , Inquéritos e Questionários , Local de Trabalho/psicologia , Violência no Trabalho/psicologia
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