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1.
J Anesth ; 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34757497

RESUMO

Low tidal volume ventilation strategy may lead to atelectasis without proper positive end-expiratory pressure (PEEP) and recruitment maneuver (RM) settings. RM followed by individualized PEEP was a new method to optimize the intraoperative pulmonary function. We conducted a systematic review and network meta-analysis of randomized clinical trials to compare the effects of individualized PEEP + RM on intraoperative pulmonary function and hemodynamic with other PEEP and RM settings. The primary outcomes were intraoperative oxygenation index and dynamic compliance, while the secondary outcomes were intraoperative heart rate and mean arterial pressure. In total, we identified 15 clinical trials containing 36 randomized groups with 3634 participants. Ventilation strategies were divided into eight groups by four PEEP (L: low, M: moderate, H: high, and I: individualized) and two RM (yes or no) settings. The main results showed that IPEEP + RM group was superior to all other groups regarding to both oxygenation index and dynamic compliance. LPEEP group was inferior to LPEEP + RM, MPEEP, MPEEP + RM, and IPEEP + RM in terms of oxygenation index and LPEEP + RM, MPEEP, MPEEP + RM, HPEEP + RM, IPEEP, and IPEEP + RM in terms of dynamic compliance. All comparisons were similar for secondary outcomes. Our analysis suggested that individualized PEEP and RM may be the optimal low tidal volume ventilation strategy at present, while low PEEP without RM is not suggested.

2.
Clin Chim Acta ; 523: 247-259, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34626603

RESUMO

BACKGROUND: Immunological monitoring plays a crucial role in organ recipients for allowing tailoring of immunosuppression. However, there is still a paucity of promising indicators for detecting immune status in recipients. METHODS: We conducted a prospective study to characterize the immune status by detecting dynamically lymphocyte subsets and function (represented by the abilities to secrete IFN-γ) in the first 6 months posttransplant in renal recipients. Participants were classified into an immune stable group, infected group, and rejected group. RESULTS: In the stable group, our study suggested that the counts and function of CD4+ T, CD8+ T, and NK lymphocytes decreased to their nadir at week 2, and thereafter these indicators were gradually restored. The counts exceeded pre-operative levels, whereas function did not reach the pre-transplant levels by 6 months. We demonstrated that function of lymphocytes was considerably decreased in infected recipients compared with the stable group when infection occurred. By contrast, the function of lymphocytes was obviously increased at the point of rejection. Receiver operating characteristic (ROC) analysis in the combination of subsets and function of lymphocytes presented a superior clinical value with an area under the curve (AUC) of 0.903 in the diagnosis of infected receivers, and IFN-γ+CD8+ T cells% is the highest indicator with the auROC curve of 0.862. Another ROC analysis confirmed that IFN-γ+CD4  T cells% presented a preferable diagnostic value with an area of 0.887 for rejected recipients. CONCLUSIONS: In conclusion, the ability of lymphocyte subsets secreting IFN-γ may provide a promising assessment of immune status in recipients and allow timely modifying immunosuppression.

3.
iScience ; 24(9): 103038, 2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34553131

RESUMO

Mitochondrial biogenesis is a cell response to external stimuli which is generally believed to suppress apoptosis. However, during the process of apoptosis, whether mitochondrial biogenesis occurs in the early stage of the apoptotic cells remains unclear. To address this question, we constructed the COX8-EGFP-ACTIN-mCherry HeLa cells with recombinant fluorescent proteins respectively tagged on the nucleus and mitochondria and monitored the mitochondrial changes in the living cells exposed to gamma-ray radiation. Besides in situ detection of mitochondrial fluorescence changes, we also examined the cell viability, nuclear DNA damage, reactive oxygen species (ROS), mitochondrial superoxide, citrate synthase activity, ATP, cytoplasmic and mitochondrial calcium, mitochondrial mass, mitochondrial morphology, and protein expression related to mitochondrial biogenesis, as well as the apoptosis biomarkers. As a result, we confirmed that significant mitochondrial biogenesis took place preceding the radiation-induced apoptosis, and it was closely correlated with the apoptotic cells at late stage. The involved mechanism was also discussed.

4.
Environ Sci Technol ; 55(18): 12459-12470, 2021 09 21.
Artigo em Inglês | MEDLINE | ID: mdl-34514800

RESUMO

Agricultural plastic films have been proven highly advantageous, but they also cause pollution of plastic debris and associated chemicals. Phthalates (phthalic acid esters, PAEs), an important additive of agricultural films, can be released and contaminate the environment. Here, we analyzed the agricultural plastic usage and assessed plastic debris in China and developed a method to estimate PAE emissions from agricultural films. Additionally, the environmental fate of PAEs was evaluated using a fugacity-based multimedia model. The agricultural plastic film usage in China in 2017 was 2,528,600 tons. After agricultural film recycling and water erosion, the plastic debris amount was estimated as 465,016 tons. The water erosion process carried 4329 tons of plastic debris into the aquatic environment. During its lifetime, the agricultural film released a total of 91.5 tons of two typical types of PAEs. PAEs from the mulching film would mostly be removed through degradation, while those from the greenhouse film accumulate in vegetables. Populated regions exhibited more serious PAE pollution in vegetables but with no immediate health risks. The model was well evaluated using comparable measured concentrations and uncertainty analysis based on the Monte Carlo method. The findings from this study demonstrate the serious agricultural plastic pollution problem and associated PAE contamination in China.


Assuntos
Ácidos Ftálicos , Poluentes do Solo , China , Ésteres , Plásticos , Poluentes do Solo/análise
5.
Sci Total Environ ; 790: 148194, 2021 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-34380251

RESUMO

Fluoroquinolones (FQs) have raised significant concerns due to their ubiquitous occurrence and promoting of antimicrobial resistance (AMR). In this study, a molecularly imprinted polymer-diffusive gradient in thin-films (MIP-DGT) sampler is developed for selective measurement of FQs in waters by using a commercial available MIP material as the binding agent. The MIP-DGT shows selective adsorption of the FQs and linearly accumulates the FQs over the deployment time. MIP-DGT measurement is independent of pH (6-8) and ionic strength (IS) (0.01-0.5 M) but is affected by DOM at higher concentrations (~10 mg•L-1), which is due to the altered diffusion coefficients and reduced adsorption on the MIP binding gel. Significant interaction effects of DOM with pH or IS indicate that this is the predominant influence on the MIP-DGT performance, which results in a lower measurement by the MIP-DGT but this is curtailed to some extend with increasing IS or pH. The MIP-DGT measurements, however, correlate well with those by grab sampling in a wastewater treatment plant, suggesting it is reliable for measuring FQs in waters. For the first time, we demonstrate that key water chemistry parameters do have interaction effects on the DGT measurements, which should be considered for the data interpretation. The MIP-DGT is a promising tool to understand the interaction effects of the environmental parameters on the fate, behaviours and bioavailability/toxicity of organic contaminants and improve environmental risk assessments in the environment and modelling.


Assuntos
Monitoramento Ambiental , Poluentes Químicos da Água , Antibacterianos/análise , Difusão , Fluoroquinolonas , Polímeros Molecularmente Impressos , Poluentes Químicos da Água/análise
6.
CNS Neurosci Ther ; 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34415671

RESUMO

INTRODUCTION: The blood-brain barrier (BBB) disruption contributes to postoperative delirium, but cost-effective and non-invasive assessment of its permeability is not practicable in the clinical settings. Urine albumin to creatinine ratio (UACR), reflecting systemic vascular endothelial dysfunction, may be a prognostic and predictive factor associated with postoperative delirium. The aim was to analyze the relationship between UACR and postoperative delirium in elderly patients undergoing elective non-cardiac surgery. MATERIALS AND METHODS: Through stratified random sampling, a cohort of 408 individuals aged 60 years and older scheduled for elective non-cardiac surgery were included between February and August 2019 in the single-center, prospective, observational study. The presence of delirium was assessed using the Confusion Assessment Method (CAM) or Confusion Assessment Method for the ICU (CAM-ICU) on the day of surgery, at 2 h after the surgery ending time and on the first 3 consecutive days with repeated twice-daily, with at least 6-h intervals between assessments. Urine samples were collected on one day before surgery, and 1st day and 3rd day after surgery. The primary outcome was the presence of postoperative delirium, and association of the level of UACR with postoperative delirium was evaluated with unadjusted/adjusted analyses and multivariable logistic regression. RESULTS: Postoperative delirium was observed in 26.75% (107 of 400) of patients within 3 days post-surgery. UACR-Pre (OR, 1.30; 95% CI, 1.14-1.49, p < 0.001), UACR-POD1 (OR, 1.20; 95% CI, 1.13-1.27, p < 0.001), and UACR-POD3 (OR, 1.14; 95% CI, 1.08-1.20, p < 0.001) between the delirium and non-delirium groups show a significant difference, even after adjusting for age, education levels, and other factors. CONCLUSION: As the marker of endothelial dysfunction, the high perioperative UACR value may be linked to the postoperative delirium in elderly patients undergoing elective non-cardiac surgery.

7.
Front Cell Dev Biol ; 9: 643525, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34249905

RESUMO

Tumor growth and metastasis are responsible for breast cancer-related mortality. Andrographolide (Andro) is a traditional anti-inflammatory drug used in the clinic that inhibits NF-κB activation. Recently, Andro has been found in the treatment of various cancers. Andro inhibits breast cell proliferation and invasion and induces apoptosis via activating various signaling pathways. Therefore, the underlying mechanisms with regard to the antitumor effects of Andro still need to be further confirmed. Herein, a MMTV-PyMT spontaneous luminal-like breast cancer lung metastatic transgenic tumor model was employed to estimate the antitumor effects of Andro on breast cancer in vivo. Andro significantly inhibited tumor growth and metastasis in MMTV-PyMT mice and suppressed the cell proliferation, migration, and invasion of MCF-7 breast cancer cells in vitro. Meanwhile, Andro significantly inhibited the expression of NF-κB, and the downregulated NF-κB reduced miR-21-5p expression. In addition, miR-21-5p dramatically inhibited the target gene expression of programmed cell death protein 4 (PDCD4). In the current study, we demonstrated the potential anticancer effects of Andro on luminal-like breast cancer and indicated that Andro inhibits the expression of miR-21-5p and further promotes PDCD4 via NF-κB suppression. Therefore, Andro could be an antitumor agent for the treatment of luminal-like breast cancer in the clinic.

8.
Huan Jing Ke Xue ; 42(7): 3147-3155, 2021 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-34212640

RESUMO

Biocides are widely added to personal care products and enter the environment through sewage treatment plant (STP) discharge, which affects ecological health. This paper evaluated the pollution characteristics of triclosan and triclocarban in a river network during the COVID-19 epidemic. Moreover, a continuous dynamic river network model coupling a one-dimensional hydrodynamic model and four-level fugacity model was established to address the temporal and spatial heterogeneity of pollutants in the river network migration process; then, this model was applied to evaluate two biocides in the Shima River Basin. The model passed calibration and in-field concentration verification tests and yielded satisfactory simulation results. The results of the study showed that the concentration of biocides in the river network during the new crown epidemic was twice that of the non-epidemic period. The concentration of triclosan and triclocarban in the river channel first increased and then decreased with the increase of the river migration distance after STP discharge. The time variation characteristics of the concentrations were affected by the river flow. The biocide concentration in the river network of the low flow upstream area first increased and then decreased, gradually stabilizing in about 20 h. The pollution concentration in the high flow downstream area was increased, and the concentration did not stabilize at 24 h. These results indicate the necessity of evaluating the temporal and spatial characteristics of migration of typical biocides in the river network by stages and time on the premise of distinguishing the flow.


Assuntos
COVID-19 , Desinfetantes , Poluentes Químicos da Água , Desinfetantes/análise , Monitoramento Ambiental , Humanos , SARS-CoV-2 , Poluentes Químicos da Água/análise
9.
Int Ophthalmol ; 41(11): 3837-3848, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34313929

RESUMO

OBJECTIVE: To investigate the role of miR-93-5p in rats with type 2 diabetic retinopathy (DR) through targeting Sirt1. METHODS: The targeting correlation between miR-93-5p and Sirt1 was validated by dual-luciferase reporter gene assay. Type 2 diabetes mellitus (T2DM) rat models were received intravitreal injection of antagomir NC (negative control), miR-93-5p antagomir, miR-93-5p agomir and/or recombinant Sirt1, followed by observation of pathological changes in retina via HE staining. Besides, retinal vascular permeability was determined by fluorescein isothiocyanate-bovine serum albumin (FITC-BSA), while the retinal vasculature was observed through retinal trypsin digestion. Expression of miR-93-5p and Sirt1 was measured by qRT-PCR and Western blotting, while the levels of VEGF, proinflammatory cytokines and anti-oxidative indicators were determined using corresponding kits. RESULTS: MiR-93-5p could target Sirt1 as analyzed by the luciferase reporter gene assay. Rats in the T2DM group presented the up-regulation of miR-93-5p and down-regulation of Sirt1 in the retina, and miR-93-5p inhibition could up-regulate Sirt1 expression in the T2DM rats. Recombinant Sirt1 decreased retinal vascular permeability and acellular capillaries with improved pathological changes in retina from T2DM rats, which was abolished by miR-93-5p agomir. Moreover, miR-93-5p inhibition or Sirt1 overexpression decreased the levels of VEGF and proinflammatory cytokines while enhancing the activity of anti-oxidative indicators. However, indicators above had no significant differences between T2DM group and T2DM + agomir + Sirt1 group. CONCLUSION: MiR-93-5p, via targeting Sirt1, could affect the vascular permeability and acellular capillaries and mitigate the inflammation and oxidative stress in the retinas, which may play a critical role in DR.


Assuntos
Diabetes Mellitus Tipo 2 , Retinopatia Diabética , MicroRNAs , Animais , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/genética , Retinopatia Diabética/genética , MicroRNAs/genética , Ratos , Sirtuína 1/genética , Sirtuína 1/metabolismo , Regulação para Cima
10.
Environ Int ; 155: 106694, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34130169

RESUMO

Anaerobic digesters have been widely used to treat wastewaters in livestock farms. With the increasing risk of antibiotic resistance originated from livestock husbandry, removal of antibiotics and antibiotic resistance genes (ARGs) via anaerobic digesters deserved more attention. Here we investigated the removal of antibiotics and ARGs in swine wastewater by three on-farm full-scale anaerobic digesters, including buried biogas digester (BBD), up-flow anaerobic sludge blanket (UASB) and high density polyethylene covered biogas digester (HDPE-BD). Variations of antibiotic resistome in swine wastewater were further revealed by metagenomic sequencing. Results showed the removal efficiencies for antibiotics, ARGs and mobile genetic elements (MGEs) varied in the three digesters, ranging from 65.1% to 98.1%, 3.5%-71.0% and 26.9%-77.2%, respectively. In general, UASB and HDPE-BD showed better removal efficiencies than BBD. However, enrichment of metal resistance genes (MRGs) was noted in UASB. Pathogens could not be effectively removed by all of the three digesters. What's more, accumulation of pathogens was found in UASB (removal efficiencies: -8.5%-13.6%). Bacterial community succession, horizontal genetic transfer and biocide and metal resistance genes (BMRGs) profiles jointly structured the variation of antibiotic resistome during anaerobic digestion. A total of 334 high-quality bins were identified from swine wastewater, 96 of which belonged to phylum of Firmicutes, Bacteroidetes and Proteobacteria carried ARGs. Proteobacteria was the dominant multi-drug resistant flora. Meanwhile, ARG-carrying pathogens (Bacteroides and Mycolicibacter) were found in the swine wastewater, suggesting a potential threat to human and animal health. The findings from this study showed that HDPE-BD is the most eco-friendly and effective anaerobic digester in controlling risks from antibiotic resistance determinants in swine wastewater.


Assuntos
Antibacterianos , Águas Residuárias , Anaerobiose , Animais , Antibacterianos/farmacologia , Genes Bacterianos/genética , Humanos , Gado , Suínos
11.
Biomolecules ; 11(5)2021 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-34062820

RESUMO

Respiratory diseases are leading causes of death and disability around the globe, with a diverse range of health problems. Treatment of respiratory diseases and infections has been verified to be thought-provoking because of the increasing incidence and mortality rate. Hydrogen sulfide (H2S) is one of the recognized gaseous transmitters involved in an extensive range of cellular functions, and physiological and pathological processes in a variety of diseases, including respiratory diseases. Recently, the therapeutic potential of H2S for respiratory diseases has been widely investigated. H2S plays a vital therapeutic role in obstructive respiratory disease, pulmonary fibrosis, emphysema, pancreatic inflammatory/respiratory lung injury, pulmonary inflammation, bronchial asthma and bronchiectasis. Although the therapeutic role of H2S has been extensively studied in various respiratory diseases, a concrete literature review will have an extraordinary impact on future therapeutics. This review provides a comprehensive overview of the effective role of H2S in respiratory diseases. Besides, we also summarized H2S production in the lung and its metabolism processes in respiratory diseases.


Assuntos
Sulfeto de Hidrogênio/metabolismo , Doenças Respiratórias/tratamento farmacológico , Sulfetos/uso terapêutico , Animais , Ensaios Clínicos como Assunto , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Doenças Respiratórias/metabolismo , Transdução de Sinais/efeitos dos fármacos , Sulfetos/farmacologia
12.
Shanghai Kou Qiang Yi Xue ; 30(1): 81-84, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33907785

RESUMO

PURPOSE: To investigate the effect of early correction with Twin-block appliance on soft and hard tissues of patients with Class Ⅱ skeletal malocclusion. METHODS: Sixty patients with Angle Class Ⅱ skeletal malocclusion were prospectively selected. The patients were divided into 2 groups, 30 cases in each group, by random number table method. Patients in the experimental group were treated with a Twin-block appliance combined with straight wire appliance, while patients in the control group were treated with a straight wire appliance. The soft and hard tissue indexes(SNA, SNB, ANB, U1-NA angle, U1-NA distance, L1-NB distance, L1-NB angle, U1-L1) before, and 12 months after treatment were measured. Soft tissue measurement indicators included UL-U1, LL-L1, over jet, overbite, E-upper-lip from the upper lip to the E-line, and the lower lip to the E-line, changes in the vertical distance (E-lower-lip), the dip angle of the sulcus, and the thickness of the soft tissue. SPSS 25.0 software package was used for statistical analysis of the data. RESULTS: U1-NA, U1-NA, L1-NB, L1-NB, overjet, overbite, E-upper-lip, and E-lower-lip were significantly less than the control group at 12 months and 36 months after treatment in the experimental group(P<0.05). U1-L1, nasolabial angle, sulcus angle, and soft tissue thickness of the experimental group were significantly greater than those of the control group(P<0.05). CONCLUSIONS: Twin-block appliance combined with straight wire appliance can significantly improve the relationship between the jaw and the soft and hard tissues of patients with Class Ⅱ skeletal malocclusion, and the long-term effect is good.


Assuntos
Má Oclusão Classe II de Angle , Má Oclusão , Sobremordida , Cefalometria , Humanos , Má Oclusão Classe II de Angle/terapia , Mandíbula
13.
Bioresour Technol ; 330: 124945, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33735733

RESUMO

The influence of copper ion (Cu2+) on anaerobic ammonium oxidation (anammox) performance and microbial community structures after oxytetracycline (OTC) stress recovery were assessed. Experimental results demonstrated that anammox performance were stressed by 1.0 mg L-1 Cu2+ and inhibitions were reversible with total nitrogen removal rate higher than 3.08 ± 0.2 kg N m-3 d-1. The residual OTC in the anammox sludge could combine with Cu2+ introduced and thereby retarded inhibition on performance in the presence of 2.0 mg L-1 Cu2+. Moreover, the positive relation of dominant bacterium Ca. Anammoxoglobus with the abundance of functional genes and parts of antibiotic resistance genes were observed, suggesting that regain of performance was the results of the gradual domestication of latent resistant species after inhibition. This investigation reveals new insights into resistance of anammox performance for Cu2+ and OTC.


Assuntos
Microbiota , Oxitetraciclina , Anaerobiose , Antibacterianos/farmacologia , Reatores Biológicos , Cobre , Resistência Microbiana a Medicamentos/genética , Nitrogênio , Oxirredução , Oxitetraciclina/farmacologia , Esgotos
14.
World J Clin Cases ; 9(6): 1490-1498, 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33644220

RESUMO

BACKGROUND: Gastrointestinal stromal tumor (GIST) usually originates in the stomach, followed by the small intestine, rectum, and other parts of the gastrointestinal tract. The most common sites of metastasis are the liver and peritoneum, whereas spinal metastases from GIST are extremely rare. CASE SUMMARY: We found a case of GIST with the first presentation of multilevel spinal metastases involving the thoracic and lumbar vertebrae. A 61-year-old Chinese man presented to our clinic because of pain in his lower back and hip for 10 d without cause. Subsequently, computed tomography (CT) and magnetic resonance imaging (MRI) revealed abnormal signals in the vertebral appendages of T12 and L4 accompanied by spinal canal stenosis, which was considered as tumor metastasis. As there were no metastases to vital organs, posterior thoracic and lumbar spinal decompression + adnexal mass resection + pedicle internal fixation was adopted to achieve local cure and prevent nerve compression. The results of histopathological studies were consistent with the metastasis of GIST. No local recurrence or new metastases were found at the 6-mo follow-up at the surgical site. The patient has no neurological symptoms at present. It is worth mentioning that a rectal mass was found and surgically removed 1 mo after the patient was discharged from hospital, and the pathological diagnosis of the mass was GIST. CONCLUSION: By reviewing 26 previously reported cases of spinal metastasis in GIST, it was found that spinal metastasis of GIST has become more common in recent years, so the possibility of early spinal metastasis should be recognized. CT and MRI are of great value in the diagnosis of spinal metastatic tumors, and pathological biopsy is the gold standard for the diagnosis of metastatic tumors. It is safe and feasible to treat isolated spinal metastasis in GIST by excising metastatic masses, decompressing the spinal canal, and stabilizing the spine.

15.
Sci Total Environ ; 772: 145558, 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-33571775

RESUMO

A comprehensive global inventory of past, present, and future steroid emissions was firstly developed based on the global 5' × 5' grids relevant data available. From 1970 to 2070, the growth rate of the annual global steroid emission was relatively stable around 10%. At present (in 2015), the global steroid emissions was 18,270 t, with 17% contributed by humans. Almost one-third of total animal emissions have been occurring in India and Brazil. India also had the highest value of human steroid emissions. Regions with highest steroid emissions were concentrated between 10° ~ 35° N and 70° ~ 90° E. The increase of sewage treatment rates can effectively reduce the total quantity of steroids entering the environment, especially for some developing countries. But the "technology bonus" from sewage treatment process will be exhausted until to 2030. Meanwhile, global surface water pollution was predicted based on steroid emissions into water compartment and on the digital river network with annual river discharge. The modelling results show that steroids are widely distributed across the globe, with concentrations mostly below 100 ng/L. However, if no proper treatment measures for animal excretions, in another 100 years, the range of the surface water contaminated by steroids will increase by 1.2 times. The Nile River resulted as the most polluted among the eight world's longest and famous rivers during the whole period investigated. Various measured concentrations worldwide validated our modelling result. The global steroid emission inventory and surface water pollution from past to the future will stand as an important data and knowledge base for the management of pollution from different types of steroids at global and regional level.


Assuntos
Gado , Poluentes Químicos da Água , Animais , Brasil , Monitoramento Ambiental , Humanos , Índia , Rios , Esteroides/análise , Poluentes Químicos da Água/análise , Poluição da Água
16.
Mol Ecol Resour ; 21(4): 1243-1255, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33421343

RESUMO

Chinese mahogany (Toona sinensis) is a woody plant that is widely cultivated in China and Malaysia. Toona sinensis is important economically, including as a nutritious food source, as material for traditional Chinese medicine and as a high-quality hardwood. However, the absence of a reference genome has hindered in-depth molecular and evolutionary studies of this plant. In this study, we report a high-quality T. sinensis genome assembly, with scaffolds anchored to 28 chromosomes and a total assembled length of 596 Mb (contig N50 = 1.5 Mb and scaffold N50 = 21.5 Mb). A total of 34,345 genes were predicted in the genome after homology-based and de novo annotation analyses. Evolutionary analysis showed that the genomes of T. sinensis and Populus trichocarpa diverged ~99.1-103.1 million years ago, and the T. sinensis genome underwent a recent genome-wide duplication event at ~7.8 million years and one more ancient whole genome duplication event at ~71.5 million years. These results provide a high-quality chromosome-level reference genome for T. sinensis and confirm its evolutionary position at the genomic level. Such information will offer genomic resources to study the molecular mechanism of terpenoid biosynthesis and the formation of flavour compounds, which will further facilitate its molecular breeding. As the first chromosome-level genome assembled in the family Meliaceae, it will provide unique insights into the evolution of members of the Meliaceae.


Assuntos
Genoma de Planta , Meliaceae , Toona , China , Cromossomos de Plantas , Malásia , Filogenia , Toona/genética
17.
Chemosphere ; 270: 128637, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33097235

RESUMO

BACKGROUND: The adverse effects of TI exposure on pregnant women are still unclear, especially regarding the risk of gestational diabetes mellitus (GDM) Objective: We explored the association between maternal urinary Tl burden and the risk of GDM. METHODS: A subsample of 1789 pregnant women were enrolled who provided spot urine samples before the diagnostic 75-g oral glucose tolerance test. Urinary Tl concentration was measured using inductively coupled plasma mass spectrometry. Logistic regression and covariance analysis were carried out to estimate the association between Tl exposure and GDM risk. RESULTS: The median of urinary Tl concentration was 0.382 µg/L or 0.525 µg/g creatinine (CC-Tl). There were 437 (24.4%) participants who were diagnosed with GDM, and the urinary CC-Tl concentrations of pregnant women with GDM were higher than that of pregnant women without GDM [0.548 (0.402, 0.788) vs 0.518 (0.356, 0.724), p = 0.014]. After adjusting for the relevant covariates, an association between urinary Tl concentrations and GDM was found. In comparison to the pregnant women in the lowest quartile of urinary CC-Tl concentration, the pregnant women in the highest quartile had a higher risk of GDM [OR (95% CI) = 1.44 (1.03, 2.02), p-trend = 0.055]. If limited to the pregnant women without family history of diabetes, the results were still robust [OR (95% CI) = 1.59 (1.11, 2.30), p-trend = 0.012]. CONCLUSION: Urinary CC-Tl concentration was associated with GDM among Chinese pregnant women. Our findings provide evidence that moderately high Tl exposure may be a novel risk factor for pregnant women health.


Assuntos
Diabetes Gestacional , China , Estudos de Coortes , Diabetes Gestacional/induzido quimicamente , Diabetes Gestacional/epidemiologia , Feminino , Humanos , Exposição Materna/efeitos adversos , Gravidez , Fatores de Risco , Tálio
18.
J Cell Physiol ; 236(3): 1658-1676, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-32841373

RESUMO

The amino acid sequence enriched with proline (P), glutamic acid (E), serine (S), and threonine (T) (PEST) is a signal-transducing agent providing unique features to its substrate nuclear proteins (PEST-NPs). The PEST motif is responsible for particular posttranslational modifications (PTMs). These PTMs impart distinct properties to PEST-NPs that are responsible for their activation/inhibition, intracellular localization, and stability/degradation. PEST-NPs participate in cancer metabolism, immunity, and protein transcription as oncogenes or as tumor suppressors. Gene-based therapeutics are getting the attention of researchers because of their cell specificity. PEST-NPs are good targets to explore as cancer therapeutics. Insights into PTMs of PEST-NPs demonstrate that these proteins not only interact with each other but also recruit other proteins to/from their active site to promote/inhibit tumors. Thus, the role of PEST-NPs in cancer biology is multivariate. It is hard to obtain therapeutic objectives with single gene therapy. An especially designed combination gene therapy might be a promising strategy in cancer treatment. This review highlights the multifaceted behavior of PEST-NPs in cancer biology. We have summarized a number of studies to address the influence of structure and PEST-mediated PTMs on activation, localization, stability, and protein-protein interactions of PEST-NPs. We also recommend researchers to adopt a pragmatic approach in gene-based cancer therapy.


Assuntos
Terapia Genética , Neoplasias/genética , Neoplasias/terapia , Proteínas Nucleares/química , Proteínas Nucleares/metabolismo , Sequência de Aminoácidos , Animais , Carcinogênese/patologia , Humanos , Neoplasias/patologia , Mapas de Interação de Proteínas
19.
J Hazard Mater ; 401: 123747, 2021 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-33113730

RESUMO

This study pays a special attention to three phenolic endocrine disrupting compounds (EDCs), - bisphenol A (BPA), 4-nonylphenol (4-NP), and 4-tert-octylphenol (4-t-OP) - that are present in urban environments, resultant of several anthropogenic activities that can be also carried through rainfall runoff. We investigated the distributions of BPA, 4-NP, and 4-t-OP in Pearl River basin and estimated the mass loads in rainfall runoff, wastewater treatment plant (WWTP) effluents, and industrial wastewater from urbanized Huizhou and Dongguan regions. These three phenolic EDCs were detected frequently in tributaries and mainstream of Dongjiang River with the maximum 4-NP concentrations of 14,540 ng/L in surface waters and 3088 ng/g in sediments. BPA showed high concentrations in rainfall runoff samples with maximum concentrations of 5873 and 2397 ng/L in Huizhou and Dongguan regions, respectively, while concentrations for 4-NP and 4-t-OP were detected at tens to hundreds of nanograms per liter. Mass loads of phenolic EDCs from rainfall runoff were 3-62 times higher than those of WWTP effluents, suggesting rainfall runoff is an important source of phenolic EDCs into receiving waters. Sources and tributaries showed median to high estrogenic risks, while low to median risks were found in mainstream, implying the source control should be focused.

20.
Bioresour Technol ; 319: 124106, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32949830

RESUMO

The individual and combined impacts of copper ion (Cu2+) and oxytetracycline (OTC) on anaerobic ammonium oxidation (anammox) performance and its self-recovery process were examined. Experimental results showed that the anammox performance and activity of anammox bacteria were inhibited by 1.0 mg L-1 OTC, Cu2+ and OTC + Cu2+, and both single and combined inhibitions were reversible. The abundance of functional genes and parts of antibiotic resistance genes (ARGs) were positively related to the dominant bacterium Ca. Kuenenia, implying that the recovery of the performance was associated with the progressive induction of potentially resistant species after inhibition. The above outcomes illustrated that anammox bacteria were stressed by metals and antibiotics, but they still could remove nitrogen at a rate higher than 20.6 ± 0.8 kg N m-3 d-1, providing guidance for engineering applications of anammox processes.


Assuntos
Compostos de Amônio , Microbiota , Oxitetraciclina , Antibacterianos/farmacologia , Reatores Biológicos , Cobre , Resistência Microbiana a Medicamentos/genética , Nitrogênio , Oxirredução , Oxitetraciclina/farmacologia
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