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1.
Taiwan J Obstet Gynecol ; 61(6): 1027-1036, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36427968

RESUMO

OBJECTIVE: The present study was aimed to identify hub genes associated with recurrent spontaneous abortion (RSA) via both bioinformatics analysis and clinical verification, also to evaluate the related pathways and immune infiltration situation of RSA, for exploring its underlying mechanism. MATERIALS AND METHODS: We screened candidate hub genes associated with RSA via bioinformatic analysis in the microarray datasets GSE22490 downloaded from the Gene Expression Omnibus (GEO) database. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were used to validate these hub genes. Several kinds of enrichment analysis were carried out to find out the pathways related to RSA. Additionally, CIBERSORT was used for evaluation of local immune Infiltration status of RSA. RESULTS: There were 536 differentially expressed genes (DEGs) including 301 upregulated and 235 downregulated genes in RSA group compared with healthy control group. Four hub genes (STAT3, TLR2, TLR4 and CD86) were finally screened out according to the protein-protein interaction (PPI) network analysis, RT-qPCR and Western blotting. Enrichment analysis showed that Toll-like receptor signaling pathway, neutrophil chemotaxis, chemokine signaling pathway and Fc gamma receptor-mediated phagocytosis were strongly associated with RSA. And in immune infiltration analysis, RSA tissue was found containing a higher proportion of monocytes and eosinophils. CONCLUSION: This study screened out four hub genes and several important pathways changed in the trophoblastic tissue of RSA patients. We also found that monocytes and eosinophils may be involved in RSA. These findings provide theoretical basis for further studies on the mechanisms of RSA.

2.
Artigo em Inglês | MEDLINE | ID: mdl-36433656

RESUMO

Searching the cost-effective organic semiconductors is strongly needed in order to facilitate the practice of organic solar cells (OSCs), yet to be fulfilled. Herein, we have succeeded in developing two non-fused ring electron acceptors (NFREAs), leading to the highest efficiency of 16.2% for the NFREA derived OSCs. These OSCs exhibit the superior operational stabilities under one sun equivalent illumination without ultraviolet (UV) filtration. It is revealed that the modulation of halogen substituents on aromatic side chains, as the new structural tool to tune the intermolecular interaction and optoelectronic properties of acceptors, not only promotes the interlocked tic-tac-toe frame of three-dimensional stacks in solid, but also improves charge dynamics of acceptors to enable high-performance and stable OSCs.

3.
Front Oncol ; 12: 998884, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36313659

RESUMO

The 7 + 3 regimen is the front-line induction chemotherapy in patients with newly diagnosed acute myeloid leukemia, with a response rate of 60-80%. But it's not suitable for all patients especially old/unfit patients because of a higher treatment related toxicity. Therefore, safer and more effective induction therapies are required. In this retrospective study, 50 patients with newly diagnosed acute myeloid leukemia received decitabine combined with HAAG (homoharringtonine, aclarubicin, low-dose cytarabine and G-CSF) as induction chemotherapy. Complete remission (CR) rate was 96% (48/50) and overall response rate was 100%. Of note, All 7 patients harboring FLT3-ITD mutation achieved CR. The median overall survival (OS) was 40.0 months (range 2.0, 58.0). The OS at 1, 3, and 5 years were 75.3%, 54.2%, and 49.3%. The median relapse free survival (RFS) was 38.0 months (range 2.0, 58.0). The RFS at 1, 3, and 5 years were 67.3%, 48.9%, and 45.1%. The OS and RFS of patients who received hematopoietic stem cell transplantation (HSCT) were significantly higher than those who did not undergo HSCT (p=0.017; 0.016). The incidence of grade 3-4 neutropenia and thrombocytopenia was 84% and 88%. Meanwhile, the incidence of grade 3-4 infection and bleeding was only 16% and 6%. There was no early death. In conclusion, DAC+HAAG regimen is effective and well-tolerated as induction therapy in patients with newly diagnosed AML.

4.
Zhongguo Zhong Yao Za Zhi ; 47(18): 5008-5021, 2022 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-36164911

RESUMO

The present study explored the main active ingredients and the underlying mechanism of Linderae Radix the treatment of gastric cancer by network pharmacology, molecular docking, and in vitro cell experiments. TCMSP, OMIM and GeneCards database were used to obtain the active ingredients of Linderae Radix to predict the related targets of both Linderae Radix and gastric cancer. After screening the common potential action targets, the STRING database was used to construct the PPI network for protein interaction of the two common targets. Enrichment analysis of GO and KEGG by DAVID database. Based on STRING and DAVID platform data, Cytoscape software was used to construct an "active ingredient-target" network and an "active ingredient-target-pathway" network. Molecular docking was performed using the AutoDock Vina to predict the binding of the active components to the key action targets, and finally the key targets and pathways were verified in vitro. According to the prediction results, there were 9 active components, 179 related targets of Radix Linderae, 107 common targets of Linderae Radix and gastric cancer, 693 biological processes, 57 cell compositions, and 129 molecular functions involved in the targets, and 161 signaling pathways involved in tumor antigen p53, hypoxia-indu-cible factor 1, etc. Molecular docking results showed that the core component, jimadone, had high binding activity with TP53. Finally, in an in vitro experiment, the screened radix linderae active ingredient gemmadone is used for preliminarily verifying the core targets and pathways of the human gastric cancer cell SGC-7901, The results showed that germacrone could significantly inhibit the proliferation of gastric cancer cells and induce the apoptosis of SGC-7901 by regulating the expression of p53, Bax, Bcl-2 and other key proteins. In summary, Radix Linderae can control the occurrence and development of gastric cancer through multi-components, multi-targets and multi-pathways, which will provide theoretical basis for further clinical discussion on the mechanism of Radix Linderae in treating gastric cancer.


Assuntos
Medicamentos de Ervas Chinesas , Lindera , Medicina Tradicional Chinesa , Farmacologia em Rede , Neoplasias Gástricas , Antígenos de Neoplasias , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Humanos , Lindera/química , Simulação de Acoplamento Molecular , Neoplasias Gástricas/tratamento farmacológico , Proteína Supressora de Tumor p53 , Proteína X Associada a bcl-2
5.
Bioresour Technol ; 363: 127948, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36108938

RESUMO

The influence of hydrazine (N2H4) and hydroxylamine (NH2OH) on performance and microbial community structures of complete autotrophic nitrogen removal over nitrite (CANON) process were assessed in this study. Experimental results demonstrated that CANON process was successfully started up and got total nitrogen removal efficiency (TNRE) of 53.6 % ± 9.8 % and 56.4 % ± 6.5 % under 1.0 and 0.5 mg L-1 N2H4 and NH2OH, respectively. N2H4 and NH2OH promoted activity of ammonia-oxidizing bacteria (AOB) and anaerobic ammonium oxidation bacteria (AnAOB), and inhibited activity of nitrite-oxidizing bacteria (NOB). Meanwhile, the stable operation of CANON process could be maintained without N2H4 auxiliary. While, performance assisted by NH2OH was fluctuated without NH2OH addition, suggesting that both N2H4 and NH2OH had a persistent and reversible inhibition on NOB. This study reveals new insights into influence of N2H4 and NH2OH on CANON performance.


Assuntos
Nitritos , Nitrogênio , Amônia , Processos Autotróficos , Bactérias , Reatores Biológicos/microbiologia , Desnitrificação , Hidrazinas/química , Hidroxilamina , Hidroxilaminas , Oxirredução
6.
Anim Biotechnol ; : 1-10, 2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36018361

RESUMO

Effects of dietary metabolizable energy (ME) and crude protein (CP) level on performance, egg quality and biochemical parameters were studied in a dual-purpose chicken-Beijing You Chicken (BYC) at peak laying period. A 3 × 3 factorial experiment was arranged, including 3 levels of dietary ME (11.31, 11.51, 11.71 MJ/kg) and 3 levels of dietary CP (14%, 15%, 16%). The results showed that dietary CP level alone and the interaction of ME by CP affected the total feed intake (TFI) during 27-30 wks, dietary ME level affected the mortality rate of 27-34 wks of age (p = 0.018), with the highest mortality rate found in 11.31 MJ/kg group (3.10%). The albumen height (AH), Haugh unit (HU) and egg grade (EG) of 16% group was higher than those in 14% and 15% groups (p < 0.05). Serum immunoglobulin G content in 11.31 MJ/kg group was lower than in 11.51 MJ/kg and 11.71 MJ/kg groups (p = 0.037). The present study suggested that dietary levels of 11.51 MJ/kg ME and 16.0% CP are enough to maintain the performance and egg quality of BYC at peak laying period. 11.31 MJ/kg ME increased the mortality of the chicken, which may be related to the decrease of the humoral immune function and antioxidative capability.

7.
Biomed Res Int ; 2022: 2305239, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35958808

RESUMO

Objective: To explore the value of early comprehensive intervention of skin contact combined with breastfeeding on improving early blood glucose in newborns with gestational diabetes mellitus (GDM). Methods: A total of 300 newborns from pregnant women with gestational diabetes who were hospitalized in Wuxi People's Hospital from January 2021 to December 2021 were randomly assigned into the observation group (n = 150) and the control group (n = 150). The former group received early comprehensive intervention of skin contact combined with breastfeeding, and the latter group received postnatal naked contact, physical examination after late navel severing, and routine nursing intervention such as early contact and early sucking in 30 min. The peripheral blood microglucose value at 1 and 2 hours after birth, neonatal hospitalization rate, ear temperature of 30 min, 60 min, 90 min, and 120 min after birth, neonatal crying, incidence of postpartum hemorrhage, uterine contraction/wound pain index, lactation before delivery, immediately after delivery, early sucking 15 min, and 2 hours postpartum were observed. Results: Compared to the control group, the values of trace blood glucose at 1 hour and 2 hours after birth in the observation group were higher, and the difference between groups was statistically significant (P < 0.05), the neonatal hospitalization rate in the observation group was lower, and the difference between groups was statistically significant (P < 0.05); the ear temperature of 30 min, 60 min, 90 min and 120 min after birth in the observation group was higher, and the difference between groups was statistically significant (P < 0.05). The crying frequency of newborns in the observation group was lower, and the difference between groups was statistically significant (P < 0.05). The incidence of postpartum hemorrhage in the observation group was lower, and the difference between groups was statistically significant (P < 0.05). The rate of uterine contraction/wound pain index grade 1 in the observation group was higher, and the difference between groups was statistically significant (P < 0.05). The rates of uterine contraction/wound pain index grade 2 and grade 3 in the observation group were lower, and the difference between groups was statistically significant (P < 0.05). The rate of lactation at 2 hours postpartum in the observation group was higher, and the difference between groups was statistically significant (P < 0.05). Conclusion: Early comprehensive intervention of skin contact combined with breastfeeding can significantly increase the early blood glucose of newborns with GDM, effectively promote the occurrence of early hypoglycemia of GDM newborns, avoid a series of serious complications caused by excessive fluctuation of blood sugar, promote the stability of vital signs of newborns, reduce the hospitalization rate of newborns, improve the success rate of breastfeeding, reduce uterine contraction/wound pain, and reduce the incidence of postpartum hemorrhage. My clinical registration number is chictr220059454.


Assuntos
Diabetes Gestacional , Hemorragia Pós-Parto , Glicemia , Aleitamento Materno , Feminino , Humanos , Recém-Nascido , Dor , Gravidez
8.
Oxid Med Cell Longev ; 2022: 8678363, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35795862

RESUMO

Thyroid cancer is considered to be one of the most common endocrine tumors worldwide. Cystathionine ß-synthase (CBS) plays a crucial role in the occurrence of several types of malignancies. And yet, the mechanism of action of CBS in the growth of thyroid carcinoma cells is still unrevealed. We found that CBS level in thyroid carcinoma tissue was higher than that in adjacent normal tissue. The overexpression of CBS enhanced the proliferation, migration, and invasion of thyroid cancer cells, while the downregulation of CBS exerted reverse effects. CBS overexpression reduced the levels of cleaved caspase-3 and cleaved poly ADP-ribose polymerase in thyroid cancer cells, whereas CBS knockdown showed reverse trends. CBS overexpression decreased reactive oxygen species (ROS) levels but increased the levels of Wnt3a and phosphorylations of phosphatidylinositol 3-kinase (PI3K), protein kinase B (PKB/AKT), mammalian target of rapamycin (mTOR), ß-catenin, and glycogen synthase kinase-3 beta, while CBS knockdown exerted opposite effects. In addition, CBS overexpression promoted the growth of xenografted thyroid carcinoma, whereas CBS knockdown decreased the tumor growth by modulating angiogenesis, cell cycle, and apoptosis. Furthermore, aminooxyacetic acid (an inhibitor of CBS) dose-dependently inhibited thyroid carcinoma cell growth. CBS can regulate the proliferation, migration, and invasion of human thyroid cancer cells via ROS-mediated PI3K/AKT/mTOR and Wnt/ß-catenin pathways. CBS can be a potential biomarker for diagnosing or prognosing thyroid carcinoma. Novel donors that inhibit the expression of CBS can be developed in the treatment of thyroid carcinoma.


Assuntos
Cistationina beta-Sintase , Neoplasias da Glândula Tireoide , Proliferação de Células/fisiologia , Cistationina beta-Sintase/metabolismo , Humanos , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Neoplasias da Glândula Tireoide/enzimologia , Neoplasias da Glândula Tireoide/patologia , beta Catenina/metabolismo
10.
Nat Ecol Evol ; 6(9): 1354-1366, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-35817827

RESUMO

Triploids are rare in nature because of difficulties in meiotic and gametogenic processes, especially in vertebrates. The Carassius complex of cyprinid teleosts contains sexual tetraploid crucian carp/goldfish (C. auratus) and unisexual hexaploid gibel carp/Prussian carp (C. gibelio) lineages, providing a valuable model for studying the evolution and maintenance mechanism of unisexual polyploids in vertebrates. Here we sequence the genomes of the two species and assemble their haplotypes, which contain two subgenomes (A and B), to the chromosome level. Sequencing coverage analysis reveals that C. gibelio is an amphitriploid (AAABBB) with two triploid sets of chromosomes; each set is derived from a different ancestor. Resequencing data from different strains of C. gibelio show that unisexual reproduction has been maintained for over 0.82 million years. Comparative genomics show intensive expansion and alterations of meiotic cell cycle-related genes and an oocyte-specific histone variant. Cytological assays indicate that C. gibelio produces unreduced oocytes by an alternative ameiotic pathway; however, sporadic homologous recombination and a high rate of gene conversion also exist in C. gibelio. These genomic changes might have facilitated purging deleterious mutations and maintaining genome stability in this unisexual amphitriploid fish. Overall, the current results provide novel insights into the evolutionary mechanisms of the reproductive success in unisexual polyploid vertebrates.


Assuntos
Carpas , Poliploidia , Animais , Genoma , Carpa Dourada/genética , Reprodução/genética
11.
Infect Genet Evol ; 103: 105307, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-35738549

RESUMO

BACKGROUND: There should be a heightened index of suspicion for Parvovirus B19 (PVB19)-related anemia in organ transplant recipients. Thus far, there is no consensus or recommendation for clinical routine monitoring methods of PVB19 recipients to allow tailoring of immunosuppression. METHODS: We conducted a retrospective study to evaluate the utility of the function (represented by the abilities to secrete IFN-γ) and numbers of lymphocyte subsets in monitoring PVB19 infections in renal recipients posttransplant. The enrolled 109 patients were split into 2 groups according to whether the recipients had an occurrence of PVB19 infection: 37 (33.94%) recipients developed PVB19 infection and 72 (66.06%) immune-stable recipients. RESULTS: The PVB19 infected group had significantly lower absolute counts and functions of different lymphocyte subsets compared with immune-stable recipients. We showed that the frequencies of IFN-γ + CD4 + T cells, IFN-γ + CD8 + T cells, and IFN-γ + NK cells increased markedly after treatment when compared to the occurrence in patients with timepoint before therapy, especially the percentages of IFN-γ + CD4 + T cells were significantly higher. Receiver operating characteristic (ROC) analysis showed that the optimal infection indicator was IFN-γ + NK cells frequency, with an auROC curve of 0.925. Concomitantly, Cox regression analysis indicated that the post-therapy increasing level of IFN-γ secreting function was significantly predictive of recurrent infections (P < 0.001). CONCLUSIONS: We recommend prospective risk stratification for the high-risk population at risk of early-onset PVB19 infection and its recurrence involves screening strategies of immune-based surveillance with the sensitive IFN-γ + secreting monitoring for antiviral prophylaxis and preemptive therapy goal. Clinical Trial Notation: clinical trial registration number: chiCTR-ROC-17010756.


Assuntos
Eritema Infeccioso , Interferon gama , Transplante de Rim , Parvovirus B19 Humano , Eritema Infeccioso/imunologia , Humanos , Interferon gama/análise , Transplante de Rim/efeitos adversos , Subpopulações de Linfócitos/imunologia , Parvovirus B19 Humano/imunologia , Estudos Retrospectivos , Transplantados
12.
Sci Total Environ ; 842: 156633, 2022 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-35716758

RESUMO

The high use of antibiotics worldwide has poses a serious threat to both human and environmental health. Lakes are served as reservoirs for antibiotics, however, there is a lack of information available on antibiotics emissions and the subsequent pollution. Here, the emission and fate of 34 frequently detected antibiotics were studied in 226 Chinese lakes, via the built emission estimation method and a temperature-dependent multimedia lake model. It has been estimated that totally 5711 tons antibiotic were discharged into the lakes of China in 2019. Antibiotics emissions are due to human activities, with 3800-fold higher emissions in the Eastern China than that in Western China. The antibiotic fate in lakes has been successfully modelled by simulating the lake stratification, freeze-melt cycles and the stable condition throughout the year. Both stratification and freeze-melt cycles can lead to increased antibiotic concentrations in lake water. Deep-water lakes were shown to serve as a highly effective natural storage medium for antibiotics. The reliability of the model was confirmed by published measured concentrations and Monte Carlo method. This is the first study to comprehensively investigate the antibiotic fate in lakes of China, providing valuable guidance for the remediation of contaminated lakes.


Assuntos
Lagos , Poluentes Químicos da Água , Antibacterianos/análise , China , Monitoramento Ambiental/métodos , Humanos , Multimídia , Reprodutibilidade dos Testes , Temperatura , Água , Poluentes Químicos da Água/análise
13.
Molecules ; 27(11)2022 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-35684331

RESUMO

Hydrogen sulfide (H2S) is an endogenous biologically active gas produced in mammalian tissues. It plays a very critical role in many pathophysiological processes in the body. It can be endogenously produced through many enzymes analogous to the cysteine family, while the exogenous source may involve inorganic sulfide salts. H2S has recently been well investigated with regard to the onset of various carcinogenic diseases such as lung, breast, ovaries, colon cancer, and neurodegenerative disorders. H2S is considered an oncogenic gas, and a potential therapeutic target for treating and diagnosing cancers, due to its role in mediating the development of tumorigenesis. Here in this review, an in-detail up-to-date explanation of the potential role of H2S in different malignancies has been reported. The study summarizes the synthesis of H2S, its roles, signaling routes, expressions, and H2S release in various malignancies. Considering the critical importance of this active biological molecule, we believe this review in this esteemed journal will highlight the oncogenic role of H2S in the scientific community.


Assuntos
Sulfeto de Hidrogênio , Neoplasias , Animais , Biologia , Cisteína , Sulfeto de Hidrogênio/metabolismo , Mamíferos/metabolismo , Neoplasias/tratamento farmacológico , Transdução de Sinais/fisiologia
14.
Front Psychiatry ; 13: 837774, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35444569

RESUMO

Objective: This study aimed to explore transcranial electrical stimulation (tES) to relieve peripartum anxiety and depressive symptoms in women undergoing cesarean section with combined spinal-epidural anesthesia. Methods: This double-blind, randomized, sham-controlled trial was conducted in the Affiliated Hospital of Xuzhou Medical University from March 2021 and May 2021. One hundred and forty-eight full-term parturients giving birth by elective cesarean section were selected, and 126 were included in the intent-to-treat analysis. Parturients were provided standardized anesthesia and randomized to the active-tES (a-tES) group and sham-tES group. Parturients and outcome assessors were blinded to treatment allocation. The primary outcome was the changes in peripartum mental health disorders, including anxiety, assessed by the Pregnancy-Related Anxiety Questionnaire-Revised 2 (PRAQ-R2). Secondary outcomes included peripartum depressive symptoms, assessed by the Edinburgh Postnatal Depression Scale (EPDS), maternal satisfaction, fatigue level, sleep quality index, and pain score during and after operation. Data were collected before entering the operating room (T0), between post-anesthesia and pre-surgery (T1), before leaving the operating room (T2), and at 24 h post-surgery (T3). Results: One hundred and twenty-six eligible parturients were enrolled in the two groups: a-tES group (N = 62) and sham-tES group (N = 64). Treatment with tES resulted in significantly lower scores of anxiety compared with sham-tES (T2: P < 0.001; T3: P = 0.001). Moreover, the a-tES groups showed a significant reduction in depression scores (T2: P = 0.003; T3: P = 0.032). Conclusion: In this randomized pilot study, tES treatment is efficacious in alleviating peripartum anxiety and depressive symptoms in women undergoing cesarean section and has been demonstrated to be a novel strategy for improving peripartum mental health disorders. Clinical Trial Registration: [www.chictr.org.cn], identifier [ChiCTR2000040963].

15.
J Tradit Chin Med ; 42(1): 96-101, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-35294128

RESUMO

OBJECTIVE: Yang-deficiency constitution (YADC) is a common unbalanced constitution that predisposes individuals to certain diseases. However, not all people with YADC manifest develop diseases. This calls for delineation of the underlying molecular mechanisms. Previous studies suggested that the gut microbiota and gene differential expression should be considered. METHODS: In the present study, we compared profiles of gut microbiota between four healthy YADC individuals and those of five healthy balanced constitution (BC) counterparts, based on 16S rRNA gene sequence analysis. Furthermore, YADC relevant genes identified by comparing 62 healthy YADC and 58 healthy BC individuals in total to perform intersection analysis, functional clustering and pathway enrichment analyses. RESULTS: The levels of harmful gut microbiota (Prevotellaceae, LDA score > 4.0, P = 0.0141) and beneficial gut microbiota (Ruminococcaceae, LDA score > 4.0, P = 0.0025, Faecalibacterium, LDA score > 4.0, P = 0.0484) were both elevated in healthy YADC individuals. Also, we found that the specific metabolic pathway with 2, 6-Dichloro-p-hydroquinone 1, 2-Dioxygenase (PcpA) as the core in gut microbiota and the glutathione transferase activity has been enriched by YADC relevant genes in healthy YADC individuals were both responsible for the detoxification of halogenated aromatic hydrocarbon substances. CONCLUSIONS: Both beneficial and harmful factors had been detected in healthy YADC individuals, functionally, they may have triggered homeostasis to maintain the health of individuals with YADC. The homeostasis may be maintained by beneficial and harmful factors from gut flora and genes. Future studies are expected to focus on halogenated aromatic hydrocarbons and their detoxification processes.


Assuntos
Microbioma Gastrointestinal , Homeostase , Humanos , RNA Ribossômico 16S/genética , Deficiência da Energia Yang
16.
17.
Sci Total Environ ; 830: 154647, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35307430

RESUMO

Antibiotics in the environment could undergo various processes with formation of transformation products, but little has been known about their occurrence and (eco)toxicological consequences. Here we investigated the occurrence and fate of nine transformation products of four tetracyclines (tetracycline, oxytetracycline, chlortetracycline and doxycycline) in three municipal wastewater treatment plants (WWTPs) in Guangzhou, China. The results showed the detection of all the tetracyclines and their transformation products in the WWTPs, with mean concentrations ranging from 17.8 ng/L (anhydrotetracycline) to 49.1 ng/L (oxytetracycline) in influent, 3.03 ng/L (tetracycline) to 6.94 ng/L (4-epi-chlortetracycline) in effluent, and 19.8 ng/g (isochlortetracycline) to 503 ng/g (4-epi-tertracycline) in sludge, respectively. The transformation products of tetracycline, oxytetracycline, chlortetracycline and doxycycline accounted for 73%-83%, 26%-52%, 70%-73% and 69%-74% of total concentrations, respectively. The aqueous removal rates of tetracyclines and their transformation products in the three WWTPs ranged from 18.4% (demethyl-chlortetracycline) to 93.7% (oxytetracycline). Mass balance analysis based on both aqueous and solid phase showed that their removals were mainly attributed to the sludge adsorption. Residual tetracyclines and their transformation products in the effluents would pose no obvious ecological risks to three aquatic organisms (green algae, daphnia and fish). However, 43.5% of sludge samples had high risks from these tetracyclines and transformation products, especially the compounds with poor biodegradability. The results from this study suggest that transformation products should be included in future environmental monitoring and control.


Assuntos
Clortetraciclina , Oxitetraciclina , Poluentes Químicos da Água , Purificação da Água , Animais , Antibacterianos/análise , Clortetraciclina/análise , Doxiciclina , Oxitetraciclina/análise , Esgotos/análise , Tetraciclina/análise , Tetraciclinas/análise , Águas Residuárias/análise , Poluentes Químicos da Água/análise
18.
Environ Pollut ; 303: 119115, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35259473

RESUMO

Fine particulate matter (PM2.5) exposure is a significant cause of chronic obstructive pulmonary disease (COPD), but the detailed mechanisms involved in COPD remain unclear. In this study, we established PM2.5-induced COPD rat models and showed that PM2.5 induced pulmonary microvascular injury via accelerating vascular endothelial apoptosis, increasing vascular permeability, and reducing angiogenesis, thereby contributing to COPD development. Moreover, microvascular injury in COPD was validated by measurements of plasma endothelial microparticles (EMPs) and serum VEGF in COPD patients. We then performed m6A sequencing, which confirmed that altered N6-methyladenosine (m6A) modification was induced by PM2.5 exposure. The results of a series of experiments demonstrated that the expression of methyltransferase-like protein 16 (METTL16), an m6A regulator, was upregulated in PM2.5-induced COPD rats, while the expression of other regulators did not differ upon PM2.5-induction. To clarify the regulatory effect of METTL16-mediated m6A modification induced by PM2.5 on pulmonary microvascular injury, cell apoptosis, permeability, and tube formation, the m6A level in METTL16-knockdown pulmonary microvascular endothelial cells (PMVECs) was evaluated, and the target genes of METTL16 were identified from a set of the differentially expressed and m6A-methylated genes associated with vascular injury and containing predicted sites of METTL16 methylation. The results showed that Sulfatase 2 (Sulf2) and Cytohesin-1 (Cyth1) containing the predicted METTL16 methylation sites, exhibited higher m6A methylation and were downregulated after PM2.5 exposure. Further studies demonstrated that METTL16 may regulate Sulf2 expression via m6A modification and thereby contribute to PM2.5-induced microvascular injury. These findings not only provide a better understanding of the role played by m6A modification in PM2.5-induced microvascular injury, but also identify a new therapeutic target for COPD.


Assuntos
Lesão Pulmonar , Doença Pulmonar Obstrutiva Crônica , Animais , Células Endoteliais/metabolismo , Humanos , Metilação , Metiltransferases/genética , Metiltransferases/metabolismo , Material Particulado/toxicidade , Doença Pulmonar Obstrutiva Crônica/induzido quimicamente , Ratos
19.
Chin J Nat Med ; 20(1): 67-73, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35101251

RESUMO

Chemical investigation of the culture extract of an endophytic Penicillium citrinum from Dendrobium officinale, afforded nine citrinin derivatives (1-9) and one peptide-polyketide hybrid GKK1032B (10). The structures of these compounds were determined by spectroscopic methods. The absolute configurations of 1 and 2 were determined for the first time by calculation of electronic circular dichroism (ECD) data. Among them, GKK1032B (10) showed significant cytotoxicity against human osteosarcoma cell line MG63 with an IC50 value of 3.49 µmol·L-1, and a primary mechanistic study revealed that it induced the apoptosis of MG63 cellsvia caspase pathway activation.


Assuntos
Neoplasias Ósseas , Osteossarcoma , Apoptose , Caspases , Humanos , Osteossarcoma/tratamento farmacológico , Penicillium
20.
Sci Total Environ ; 822: 153177, 2022 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-35090918

RESUMO

The application of animal manure containing antibiotic residues as an organic fertilizer to farmlands, poses a major threat to the health of river basin ecosystems. Waste treatment processes can help reduce antibiotic pollution levels in river basins following manure application, but the overall influence of these processes remains unclear. This study evaluates the impact of manure treatment methods on the emission and subsequent river pollution caused by 14 frequently detected antibiotics in a typical pig breeding area in China, by using hypothetical scenarios method. Three scenarios were constructed based on possible fate pathways of antibiotics, representing in 47.0, 55.3, and 81.6 ton·yr-1 antibiotic emissions into the river basin. The soil and water assessment tool (SWAT) model successfully simulated the transport of antibiotics from farmland to surface water, with calibration and verification performed using hydrological station monthly data over 8 consecutive years. Field measured concentrations also verified the reliability of the model and were used to determine the most realistic scenario. In basins applied with manure, environmental antibiotic pollution is most affected by the wastewater treatment process and manure applied patterns, followed by changes in streamflow. The antibiotic pollution in manure applied areas showed significant spatial and temporal differences, resulting from the different manure application patterns. The simulated total outflow of antibiotics in the river basin accounted for 18.1% of the inflow, with the loss of target antibiotics by degradation, volatilization and sedimentation deposition in the river basin being 0.23, 0.01 and 33.2 ton·yr-1, respectively. This study can help to clarify the environmental fate of antibiotics in the basin following manure application, provide guidance for policy makers and help to design the effective corrective interventions for reducing the environmental pollution.


Assuntos
Antibacterianos , Esterco , Animais , Antibacterianos/análise , China , Ecossistema , Monitoramento Ambiental , Poluição Ambiental , Reprodutibilidade dos Testes , Rios , Solo , Suínos
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