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1.
J Asian Nat Prod Res ; : 1-7, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34190010

RESUMO

Two new furoquinoline alkaloids, named 1'-oxo-isoplatydesmine (1) and demethoxyacrophylline (2), as well as 11 known alkaloids (3-13) were isolated from the root bark of Dictamnus dasycarpus Turcz. The structures of 1 and 2 were established by detailed spectroscopic elucidation, such as 1 D & 2 D NMR and HRMS, etc. The unexpected autoracemization of 1 was discussed based on the stereochemistry of reported dihydrofuroquinolines. Compounds 3-5 exhibited moderate inhibitory activities against Bacillus subtilis, Candida albicans, and Pseudomonas aeruginosa with MICs 32-64 µg/ml, revealing the active principles of D. dasycarpus for treating skin diseases in its traditional usage.

2.
BMC Musculoskelet Disord ; 22(1): 442, 2021 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-33990209

RESUMO

BACKGROUND: Modular prosthesis fracture, especially distal femoral fracture, is a rare complication of total hip arthroplasty (THA). However, it is catastrophic, and may have a serious impact on the patients. A distal femoral prosthesis fracture in a patient with developmental dysplasia of the hip (DDH) with nonunion at the subtrochanteric osteotomy site has not yet been reported in any literature. This report presents the first such case, with a purpose of analyzing the causes of modular prosthesis fractures and nonunion of the osteotomy area, so as to provide orthopedic surgeons with experience and lessons. CASE PRESENTATION: We report the case of a 52-year-old woman with the distal femoral prosthesis fracture after THA and subtrochanteric osteotomy for Crowe type IV DDH. The patient had severe pain in the left thigh and her activities were limited. Plain radiographs revealed fracture of the left distal femoral prosthesis and nonunion in the subtrochanteric osteotomy region of the left femur. After a revision of the THA, the patient's symptoms were resolved. CONCLUSIONS: A prosthesis fracture combined with nonunion at the subtrochanteric osteotomy site is a rare complication. Modular THA combined with a subtrochanteric osteotomy in the treatment of Crowe type IV DDH should reduce the damage to blood supply and avoid further nonunion of the osteotomy area, which may otherwise lead to modular prosthesis fractures. A detailed preoperative plan and suitable rehabilitation program may help minimize the occurrence of subtrochanteric osteotomy nonunion and reduce complications, including femoral prosthesis fractures, in patients with DDH.


Assuntos
Artroplastia de Quadril , Displasia do Desenvolvimento do Quadril , Luxação Congênita de Quadril , Prótese de Quadril , Artroplastia de Quadril/efeitos adversos , Feminino , Fêmur/cirurgia , Luxação Congênita de Quadril/diagnóstico por imagem , Luxação Congênita de Quadril/cirurgia , Prótese de Quadril/efeitos adversos , Humanos , Pessoa de Meia-Idade , Osteotomia , Complicações Pós-Operatórias , Estudos Retrospectivos
3.
Phytochem Anal ; 32(2): 198-205, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32519355

RESUMO

INTRODUCTION: Emerging network pharmacology (NP) combines phytochemical information with bioinformatics tools allowing herbal formulae to be illustrated holistically in the context of phytochemical basis and therapeutic mechanisms. OBJECTIVE: This study attempted to explore the holistic molecular evidence of herbal formula Si-Wu decoction (SWD) by using the method of NP. MATERIAL AND METHOD: Databases of traditional medicines combined with PubChem, SciFinder, SEA, STRING, and KEGG were employed to gather information for establishing the "compound similarity" (CS) network and the "target-(pathway)-target" (TPT) network. Gephi software was applied to visualise the networks, with further module-based and node-based network topological analysis. Moreover, the approved drugs and shortest path analysis were used to validate the TPT network. RESULTS: The CS network presented the phytochemical profile of SWD, including the major compound groups of iridoid glycosides, glycosides, phthalide lactones, phenylpropanoids, and monoterpenoids. Furthermore, the topological analysis of TPT network depicted the holistic property of SWD in interpretable neuroendocrine immunomodulation (NIM) perspective, and the node degree analysis indicated a closer connection of SWD with endocrine or metabolism system. Moreover, by combing the analysis of the CS network and TPT network, potential active ingredients could be primarily identified. CONCLUSION: The phytochemical profile and molecular target profile, which might pave the way for an understanding of SWD in modern science and provide a reference for relevant quality research and evaluation, were demonstrated by network analysis. Moreover, the methods could be further applied to discover the phytochemical or biomolecular evidence with distinct advantages in dealing with the tremendous separated information.


Assuntos
Medicamentos de Ervas Chinesas , Compostos Fitoquímicos
4.
Biomed Pharmacother ; 114: 108863, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30991286

RESUMO

Yinchenhao decoction (YCHD) is a classical Traditional Chinese Medicine (TCM) formula that has been widely used in the treatment of liver fibrosis caused by chronic hepatitis B and jaundice for more than 1800 years. The purpose of this study was to investigate the apoptosis regulation mechanisms of YCHD and its active components suppresses liver fibrosis. The active components and putative targets of YCHD were predicted by network pharmacology approach. Functional and pathway enrichment analysis were presented in the present study by using clusterProfiler. Further, experimental validation was done by using terminal deoxynucleotidyl transferase (TDT) dUTP nick end labelling (TUNEL) assay and western blotting in dimethylnitrosamine (DMN)-induced liver fibrosis rats, and cell proliferation assay, apoptosis assay, and western blotting in human hepatic L02 cells and LX2 cells. 45 active compounds in YCHD formula, 592 potential target proteins and 1191 liver fibrosis-related human genes were identified. Functional and pathway enrichment analysis indicated that YCHD obviously influenced TNF, PI3K-Akt signaling pathways. Further, In vivo experiment indicated that YCHD treatment not only attenuated the symptoms of liver fibrosis, but also decrease the apoptosis of hepatic parenchyma cells. Moreover, in vitro experiments showed that rhein, kaempferol and quercetin treatments remarkably decreased the protein levels of cleaved caspase-3 and increased p-ERK1/2, PI3K and Bcl-XL protein expression in TNF-α-stimulated L02 cells. On the contrary, rhein, kaempferol, aloe-emodin and quercetin inhibited the proliferation of LX2 cells and up-regulated the protein levels of Bax and cleaved caspase-8. In conclusion, 45 active components and 296 potential targets of YCHD against liver fibrosis were identified by the analysis of network pharmacology and transcriptomics combination. The mechanisms of YCHD against liver fibrosis were involved in the regulation of multiple targets, especially affecting the apoptosis-related signaling pathways.


Assuntos
Apoptose/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Cirrose Hepática/tratamento farmacológico , Animais , Caspase 3/metabolismo , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Perfilação da Expressão Gênica/métodos , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Humanos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Cirrose Hepática/metabolismo , Masculino , Medicina Tradicional Chinesa/métodos , Fosfatidilinositol 3-Quinases/metabolismo , Ratos , Ratos Wistar , Proteína bcl-X/metabolismo
5.
J Ethnopharmacol ; 228: 200-209, 2019 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-30240786

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Siegesbeckiae Herba (SH) is a traditional anti-rheumatic herbal medicine in China. The SH-derived product is the first licensed traditional herbal medicinal product for the management of rheumatism-induced joint and muscle pain in United Kingdom. The authenticated plant origins listed in the official Chinese Pharmacopeia for SH include Siegesbeckia orientalis L. (SO), S. pubescens Markino (SP) and S. glabrescens Markino (SG). Although the therapeutic effects of these SH species in treating rheumatoid arthritis (RA) are similar, their difference in chemical profiles suggested their anti-rheumatisms mechanisms and effects may be different. AIM OF THE STUDY: This study was designed to comparatively comprehend the chemical and biological similarity and difference of SO, SP and SG for treating rheumatoid arthritis based on the combination of computational predictions and biological experiment investigations. MATERIALS AND METHODS: The reported compounds for SO, SP and SG were obtained from four chemical databases (SciFinder, Combined Chemical Dictionary v2009, Dictionary of Natural Products and Chinese academy of sciences Chemistry Database). The RA-relevant proteins involved in nuclear factor-kappa B (NF-κB), oxidative stress and autophagy signaling pathways were collected from the databases of Kyoto Encyclopedia of Genes and Genomes and Biocarta. The comparative comprehension of SH plants was performed using similarity analysis, molecular docking and compounds-protein network analysis. The chemical characterization of different SH extracts were qualitatively and quantitatively analyzed, and their effects on specific RA-relevant protein expressions were investigated using Western blotting analysis. RESULTS: Chemical analysis revealed that SO contains mainly sequiterpenes and pimarenoids; SP contains mainly pimarenoids, sequiterpenes, and kaurenoids; and SG contains mainly pimarenoids, flavonoids and alkaloids. Moreover, coincided with the predicted results from computational analysis, different SH species were observed to present different chemical constituents, and diverse effects on RA-relevant proteins at the biological level. CONCLUSIONS: The chemical and biological properties of SO, SP and SG were different and distinctive. The systematic comparison between these three confusing Chinese herbs provides reliable characterization profiles to clarify the pharmacological substances in SH for the precise management of rheumatism/-related diseases in clinics.


Assuntos
Antirreumáticos , Asteraceae , Medicamentos de Ervas Chinesas , Animais , Antirreumáticos/química , Asteraceae/química , Medicamentos de Ervas Chinesas/química , Lipopolissacarídeos , Camundongos , Simulação de Acoplamento Molecular , Fitoterapia , Proteínas de Plantas/análise , Células RAW 264.7 , Especificidade da Espécie
6.
Ying Yong Sheng Tai Xue Bao ; 27(5): 1628-1638, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-29732826

RESUMO

The objective of this study was to evaluate the molecular characteristics of heat shock protein 70 (HSP70) gene, and document changes in HSP70 gene expression upon exposure of Perinereis aibuhitensis to aqueous Cu2+. Full length cDNA of HSP70 was sequenced and the characteristics of the translated protein were determined and compared both with other ragworms species and other invertebrates. The results showed that the cDNA was 2161 bp and consisted of a 5'-terminal untranslated region (UTR) of 48 bp, a 3'-termianl UTR of 142 bp with a canonical polyadenylation signal sequence AATAAA and a poly A tail, and an open reading frame (ORF) of 1971 bp. The HSP70 protein was 656 amino acids with a calculated molecular weight of 71.43 kD and theore-tical isoelectric point of 5.15. Sequence analysis of the protein showed that HSP70 of P. aibuhitensis contained three signature sequences IDLGTTYS, IFDLGGGTFDVSIL and IVLVGGSTRIPKIQK all belonging to the HSP70 family, a cytoplasm characteristic motif of EEVD, and the C-terminal repeats of GGMP. The molecular characteristics of P. aibuhitensis shared 94% identity with other ragworms and 79% identity with other invertebrates. In ragworms exposed to Cu2+, expression of HSP70 mRNA increased significantly with Cu2+ concentration (0.2-5.0 mg·L-1), and it reached the peak on 1-day exposure. In summary, the molecular characteristics of HSP70 of P. aibuhitensis were described, and its gene expression was inducible by exposure to aqueous Cu2+, suggesting HSP70 has potential to become a useful molecular biomarker of environmental pollution.


Assuntos
Proteínas de Choque Térmico HSP70/genética , Poliquetos/genética , Sequência de Aminoácidos , Animais , Clonagem Molecular , DNA Complementar , Filogenia , RNA Mensageiro
7.
Huan Jing Ke Xue ; 36(7): 2459-64, 2015 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-26489312

RESUMO

Nitrogen is the most comment source of eutrophication in freshwater systems. In current study, we investigated the various forms of transferable nitrogen in sediments, which potentially contributed to the nitrogen output into waters. Sediments samples were collected in the water level fluctuating zone of Pengxi River crossing three hydrological sections, e. g. upstream, midstream and downstream and two water level altitudes, 160 m and 170 m, with multiple depths for each site, 0-20, 20-40, 40-60, 60-80, 80-100 cm. To characterize the response of transformation of nitrogen in sediment of the water level fluctuating zone towards cycles of drought and re-wetting processes, we analyzed the content and distribution of transferable nitrogen (TF-N) and its forms. The result showed that the changing of the amount of total nitrogen followed the pattern of upstream > midstream > downstream, the mean value was in the range of 313.02-3 255.53 mg. kg-1, while the content of total transferable nitrogen was on an average of 639.40 mg . kg-1 and coincided with the pattern of total nitrogen. In addition, TF-N followed the pattern of OSF-N > IMOF-N > OSF-N > IEF-N. It indicated that both OSF-N and IMOF-N dominated the form of TF-N in sediments by proportions of 50. 9% and 33. 3%, respectively. Since the transformation rate of OSF-N into dissolved phase was relatively slow, its contribution as a source of nitrogen to eutrophication was limited. We, thus, concluded that IMOF-N was the most important fraction which could be potentially affected by water fluctuation and contributed as dissolved nitrogen into water phase. This study indicated that the manner of manipulating water resource in the Three Gorges Reservoir area has raised the potential risk of transforming IMOF-N from sediment into water phase.


Assuntos
Secas , Sedimentos Geológicos/química , Nitrogênio/análise , Rios/química , Poluentes Químicos da Água/análise , China , Eutrofização , Movimentos da Água
8.
Org Lett ; 16(6): 1684-7, 2014 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-24597609

RESUMO

The N-S bond-based internal oxidant offers a distinct approach for the synthesis of highly functionalized pyridines. A novel Rh(III)-catalyzed one-pot process undergoes an efficient C-C/C-N bond formation along with desulfonylation under very mild conditions. The method is quite simple, general, and efficient.


Assuntos
Alcinos/química , Iminas/química , Nitrilas/química , Oxidantes/química , Piridinas/síntese química , Sulfonas/química , Catálise , Técnicas de Química Combinatória , Estrutura Molecular , Piridinas/química , Ródio/química
9.
Zhongguo Zhong Yao Za Zhi ; 39(22): 4418-25, 2014 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-25850278

RESUMO

In this study, researchers adopted the network analysis method to study Buyang Huanwu decoction at three levels, namely chemical ingredients, targets and diseases, and discovered the potential effect of Buyang Huanwu decoction in cancer treatment. Besides, they analyzed the "target-target" network of Buyang Huanwu decoction based on diseases, calculated four network indexes, namely node centrality, closeness centrality, betweenness centrality and eigenvector centrality for a comprehensive evaluation on the importance and significance of each target in the network. Afterwards, key targets of Buyang Huanwu decoction were excavated to obtain two important targets--COX-2 and PPAR-gamma, which may be important targets involved in the qi deficiency and blood stasis diseases. Meanwhile, the two targets were the basis to build the core network of "chemical component-target-disease" of Buyang Huanwu decoction, which provided reference for further studies on the effect of Buyang Huanwu decoction in treating qi deficiency and blood stasis diseases. According to the study, the network analysis method was helpful to excavate potential targets Buyang Huanwu decoction in treating qi deficiency and blood stasis diseases, and could provide methodological reference for revealing the mechanism of Buyang Huanwu decoction at multiple levels, with a guiding significance for interpreting mechanisms of traditional Chinese medicinal formulae and developing new drugs.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Doenças Hematológicas/tratamento farmacológico , Deficiência da Energia Yang/tratamento farmacológico , Deficiência da Energia Yin/tratamento farmacológico , Medicina Tradicional Chinesa , Qi
10.
Ying Yong Sheng Tai Xue Bao ; 25(9): 2683-94, 2014 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-25757323

RESUMO

Ragworm (Polychaeta) is an ecologically important species in intertidal mudflats and estuaries and is recognized as a sentinel species for environmental monitoring. In the intertidal belt of China, the ragworm Perinereis aibuhitensis is frequently exposed to various toxicants including cadmium (Cd) and petroleum hydrocarbons (PHCs). The present study investigated the influence of PHCs on accumulation of Cd and the induction of metallothionein (MT) in P. aibuhitensis by means of chronic microcosm experiment in which the ragworms were exposed to Cd or combinations of Cd and PHCs. The accumulation of Cd in P. aibuhitensis increased significantly with the Cd exposure concentration when exposed to Cd alone. Further, the bioconcentration factors (BCFs) for Cd in P. aibuhitensis increased with the duration of exposure. The addition of PHCs in Cd exposure solutions significantly increased the accumulation of Cd in P. aibuhitensis, compared with the control. Exposure to Cd induced the expression of MT, and the expression increased with the concen- tration up to 180 mg · kg(-1) DM Cd at which point the level of induction did not increase. PHCs without Cd did not significantly induce MT in P. aibuhitensis, but the addition of PHCs with Cd did influence the induction of MT compared to Cd without PHCs. Results indicated that PHCs could modulate the expression of MT during co-exposure with Cd. The potential substances other than metals influencing the interpretation of MT expression in wild P. aibuhitensis must be recognized when MT is used as a monitoring index.


Assuntos
Cádmio/metabolismo , Metalotioneína/metabolismo , Petróleo , Poliquetos/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , China , Monitoramento Ambiental , Hidrocarbonetos , Metais
12.
Org Biomol Chem ; 11(36): 6142-9, 2013 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-23925493

RESUMO

A simple approach for synthesis of novel aza-fused scaffolds such as pyrido[1,2-α]benzimidazoles and imidazo[1,2-α]pyridines was developed by Rh(III)-catalyzed direct oxidative coupling between alkenes and unactivated alkynes without an extra directing group. The method would allow a broad substrate scope, providing fused heterocycles with potential biological properties.


Assuntos
Benzimidazóis/síntese química , Compostos Organometálicos/química , Piridinas/síntese química , Ródio/química , Benzimidazóis/química , Catálise , Estrutura Molecular , Piridinas/química
13.
Org Lett ; 15(8): 1878-81, 2013 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-23537406

RESUMO

Double C-H activations of C(5)-H and Csp(2)-H of 2-substituted N-vinyl- or arylimidazoles were realized without heteroatom-directing assistance by rhodium(III) catalyst. A subsequent oxidative annulation reaction with alkynes efficiently produced aza-fused heterocycles with high molecular complexity in low to excellent yields.


Assuntos
Alcinos/química , Compostos Heterocíclicos/síntese química , Imidazóis/química , Ródio/química , Catálise , Ciclização , Compostos Heterocíclicos/química , Estrutura Molecular , Oxirredução , Estereoisomerismo
14.
Zhonghua Yan Ke Za Zhi ; 48(1): 52-60, 2012 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-22490917

RESUMO

OBJECTIVE: To investigate the influence of vascular endothelial growth factor(VEGF) and transforming growth factor (TGF)-ß(2) to human retinal pigment epithelium(RPE) cell differentiation, and the mechanism of collagen gel contraction mediated by RPE cells. METHODS: Experiment study. An in vitro collagen gel contraction assay was performed to evaluate the effect of cultured human RPE in addition of VEGF and TGF-ß(2) at indicated time points (24 h, 48 h and 72 h). Three groups were established in the experiment:control group, 50 µg/L VEGF group and 5 µg/L TGF-ß(2) group. The effects of both cytokines on the collagen gel contraction were analyzed by the reduced diameter of the collagen gel. And the changes of cell morphology and their transdifferentiation were assessed to estimate the possible connection between RPE transdifferentiation and collagen gel contraction. One-way ANOVA was used in conjunction with SNK-q test to assess statistical significance at different time periods within groups. RESULTS: There were differences on collagen gel contraction rates among VEGF group [(34.7 ± 3.1)%, (44.3 ± 6.0)%, (44.0 ± 7.2)%], TGF-ß(2) group [(29.3 ± 3.1)%, (31.7 ± 3.5)%, (29.0 ± 3.6)%] and control group [(20.0 ± 0.5)%, (17.3 ± 3.6)%, (19.1 ± 0.8)%] at 24 h, 48 h and 72 h after cultured (24 h: F = 26.220, P = 0.001; 48 h: F = 26.796, P = 0.001; 72 h: F = 21.522, P = 0.002), and on each time point two two comparison in the three groups (SNK-q test, P < 0.05). There were differences on protein expression level of α-smooth muscle actin (α-SMA) in 50 µg/L VEGF group and 5 µg/L TGF-ß(2) group at difference time points, respectively (TGF-ß(2) group: F = 1.134, P = 0.000; each time point: SNK-q test, P < 0.05; VEGF group: F = 279.179, P = 0.000; each time point: SNK-q test, P < 0.05). Moreover, TGF-ß(2) (5 µg/L) demonstrated stronger and more permanent gel contraction than VEGF (50 µg/L) (6 h: F = 3.646, P = 0.000; 24 h: F = 18.706, P = 0.003; 48 h: F = 124.195, P = 0.000; 72 h: F = 76.811, P = 0.000). RPE cells' form happened fibroblasts sample transformation in both VEGF group and TGF-ß(2) group. CONCLUSIONS: Both VEGF and TGF-ß(2) can induce the collagen gel contraction, partly by means of inducing the expression of α-SMA and RPE contraction, which may thus contribute to the explanations of vitro-retinal diseases.


Assuntos
Epitélio Pigmentado da Retina/efeitos dos fármacos , Fator de Crescimento Transformador beta2/farmacologia , Fator A de Crescimento do Endotélio Vascular/farmacologia , Actinas/metabolismo , Células Cultivadas , Colágeno/metabolismo , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Humanos , Epitélio Pigmentado da Retina/citologia
15.
Zhonghua Yan Ke Za Zhi ; 47(11): 1012-8, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22336068

RESUMO

OBJECTIVE: To evaluate the role of vascular endothelial growth factor or hypoxia on the secretion of opticin in retinal pigment epithelium cells. METHODS: Human RPE cells were cultured, the third to sixth passage of retinal pigment epithelium (RPE) cells were placed in 6-well culture plates at a density of 4 × 10(4)/well. For hypoxia experiment, the cells were cultured under hypoxic condition for different times. For vascular endothelial growth factor (VEGF) experiment, the media was changed to DMEM containing different concentration VEGF (1, 10, 50, 100 µg/L) for 24 h respectively. VEGF mRNA levels were determined by RT-RCR method. The protein content of opticin in RPE cells or culture media was detected by Western blot. Matrix metalloproteinase activity in culture media was analysis by zymography. One way ANOVA was used to test the comparisons between experimental groups and control group. RESULTS: Western blot experiment showed the opticin expression was not changed in RPE cells after hypoxia treatment, however was significantly decreased in culture media. Compared with control group (0.21 ± 0.03). The relative density of VEGF mRNA levels (0.81 ± 0.04, 0.67 ± 0.07) in RPE cells were increased after 12 h or 24 h hypoxia treatment (F = 483.60, P < 0.05). Opticin expression in RPE cells was also remain unchanged after vary concentration VEGF addition treatment (F = 2.16, P > 0.05), the relative density of opticin expression in VEGF conditioned culture medium were 0.65 ± 0.02, 0.52 ± 0.04, 0.23 ± 0.03, 0.30 ± 0.03 respectively, and the difference in culture media was significant compared to control group (0.73 ± 0.04) (F = 141.38, P < 0.05). Zymography indicate a matrix metalloproteinases type 2 digest band, the activities were enhanced with VEGF increasing. The decrease of opticin in culture media after VEGF treatment could be inhibited by low condition of EDTA. CONCLUSION: VEGF and hypoxia have an effect on the on the secretion of opiticin in RPE cells, it may be contributed to the increasing levels of matrix metalloproteinases type 2.


Assuntos
Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Proteínas da Matriz Extracelular/metabolismo , Proteoglicanas/metabolismo , Epitélio Pigmentado da Retina/citologia , Fator A de Crescimento do Endotélio Vascular/farmacologia , Hipóxia Celular , Células Cultivadas , Humanos , Epitélio Pigmentado da Retina/efeitos dos fármacos , Epitélio Pigmentado da Retina/metabolismo
16.
Zhongguo Zhen Jiu ; 30(5): 375-8, 2010 May.
Artigo em Chinês | MEDLINE | ID: mdl-20518172

RESUMO

OBJECTIVE: To compare the clinical efficacy of osteoarthritis of knee joint treated by acupuncture and moxibustion and simple acupuncture. METHODS: Sixty-two cases were randomized into an observation group (32 cases) and a control group (30 cases). In the observation group, acupuncture and non-scarring moxibustion were applied. Acupuncture was applied on the local acupoints of knee and moxibustion was performed on Shenshu (BL 23) and Xuehai (SP 10). In the control group, only acupuncture was adopted. The clinical efficacy was observed after 2 courses of treatment. Lysholm knee joint motor function scale, visual analogue scale (VAS) and WHO quality of life (WHOQOL-BREF) were used for the assessment of scores before and after treatment and the statistical analysis of clinical efficacy. RESULTS: The total effective rate (93.8%, 30/32) in the observation group was superior to that (87.7%, 26/30) in the control group (P < 0.05). The scores in Lysholm knee joint motor function scale and VAS were improved after treatment compared with those before treatment in two groups (P < 0.05, P < 0.01). The degree of improvement in the observation group was superior to that in the control group (P < 0.05, P < 0.01). There was no statistical significance in the scores of WHOQOL-BREF before and after treatment in two groups as well as in intra-group comparison (all P > 0.05). CONCLUSION: Acupuncture and moxibustion in combination achieve the definite clinical efficacy on osteoarthritis of knee joint and this therapy is superior to simple acupuncture in the improvement of motor function of knee joint and the alleviation of pain.


Assuntos
Terapia por Acupuntura/métodos , Moxibustão/métodos , Osteoartrite do Joelho/terapia , Idoso , Feminino , Humanos , Masculino , Medicina Tradicional Chinesa , Pessoa de Meia-Idade , Osteoartrite do Joelho/psicologia , Medição da Dor , Qualidade de Vida
17.
Ying Yong Sheng Tai Xue Bao ; 19(3): 641-6, 2008 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-18533538

RESUMO

This paper studied the effects of applying citric acid, oxalic acid and acetic acid on the removal of heavy metals Cd, Pb, Cu and Zn from sewage sludge, and the form transformation and bioavailability of these heavy metals before and after the application of tested organic acids. The results showed that the removal rates of tested heavy metals except Cu increased with increasing acid concentration and action time. Among the tested organic acids, citric acid was more effective in removing heavy metals from sewage sludge, with the removal rate of 52.0% for Pb and 74.2% for Zn after a 7-hour action, and 76.0% for Pb and 92.5% for Zn after 24-hour action. The removal rates of the heavy metals by oxalic acid and acetic acid were relatively low. The Pb and Zn removed by citric acid were mainly in stable form, resulting in a relative increase of their unstable and exchangeable forms in the sludge. Though the removal rate of Cu and Cd was lower, their exchangeable forms still had somewhat increase after the action of tested organic acids.


Assuntos
Ácido Acético/química , Ácido Cítrico/química , Metais Pesados/química , Ácido Oxálico/química , Esgotos/química , Cádmio/química , Cádmio/isolamento & purificação , Cobre/química , Cobre/isolamento & purificação , Chumbo/química , Chumbo/isolamento & purificação , Metais Pesados/isolamento & purificação , Eliminação de Resíduos Líquidos/métodos , Zinco/química , Zinco/isolamento & purificação
18.
Ying Yong Sheng Tai Xue Bao ; 18(9): 2085-90, 2007 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-18062318

RESUMO

This paper studied the removal effect of citric acid, oxalic acid and acetic acid on cadmium (Cd), lead (Pb), copper (Cu) and zinc (Zn) in sewage sludge, and the changes of NO3(-)-N and NH4(+)-N concentrations in extracted liquid and sludge before and after treated. The results showed that 0.8 mol x L(-1) of citric acid had better effect, which could remove 76.0% of Pb and 92.5% of Zn in the sludge. After treated with test organic acids for 24 hours, large amounts of NO3(-)-N and NH4(+)-N dissolved in extracted liquid. Compared with the control (distilled water), organic acids greatly increased the NH4(+)-N, but decreased the NO3(-)-N in extracted liquid. Because of the transformation of other forms nitrogen in the process of acid treatment, the NO3(-)-N and NH4(+)-N concentrations in the treated sludge were still high. The extracted liquid after the sludge was treated with 0.5 mol x L(-1) of oxalic acid contained 2.8 mg x L(-1) of NO3(-)-N and 888.1 mg x L(-1) of NH4(+)-N but a few of heavy metals, which was able to be reused as a liquid fertilizer.


Assuntos
Metais Pesados/análise , Nitratos/análise , Compostos de Amônio Quaternário/análise , Esgotos/química , Ácidos/química , Metais Pesados/isolamento & purificação , Nitratos/química , Compostos Orgânicos/química , Compostos de Amônio Quaternário/química , Eliminação de Resíduos Líquidos
19.
Huan Jing Ke Xue ; 28(8): 1796-801, 2007 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-17926413

RESUMO

Using the indoor simulating method of pollution exposure at various concentrations, the toxic effects of various concentrations of petroleum hydrocarbons (PHCs), copper (Cu2+) and cadmium (Cd2+) on the polychaete Perinereis aibuhitensis Grube and on its acetycholinesterase (AChE) activity were examined. The results indicated that petroleum hydrocarbons, Cu2+ and Cd2+ had the high toxicity to the polychaete Perinereis aibuhitensis Grube. After 4-day and 10-day exposure to petroleum hydrocarbons, Cu2+ and Cd2+, the value of LC50 were 440 and 110 microg x L(-1) for PHCs, 1 150 and 570 microg x L(-1) for Cu2+, 5 090 and 2 500 microg x L(-1) for Cd2+. In particular, PHCs had a stronger acute toxic effect than Cu and Cd. The AChE activity was inhibited, when the animal was exposed to Cd2+ and Cu2+, but the rate of inhibition was less than 50%. In particular, the AChE activity of the animal exposed to PHCs was significantly inhibited. The maximum rate of the inhibition by PHCs reached more than 90%. Moreover, the changes in the AChE activity significantly related with the concentration of PHCs. Thus, the index in the activity of AChE can sensitively reflect the toxic effects of PHCs on Perinereis aibuhitensis Grube as an important biomarker.


Assuntos
Acetilcolinesterase/metabolismo , Cobre/toxicidade , Hidrocarbonetos Aromáticos/toxicidade , Resíduos Industriais , Poliquetos/efeitos dos fármacos , Animais , Cádmio/toxicidade , Hidrocarbonetos Aromáticos/metabolismo , Petróleo/toxicidade , Poliquetos/enzimologia , Poliquetos/crescimento & desenvolvimento , Testes de Toxicidade/métodos
20.
Huan Jing Ke Xue ; 28(4): 826-31, 2007 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-17639945

RESUMO

In order to explore the combined ecological effects of acetochlor and copper sulphate on soil microorganisms, the traditional toxicological methods and BIOLOG were employed. The results indicate that the combined application of acetochlor and copper sulphate has acute inhibitory effects on the amount of culture-dependent viable bacteria, actinomycetes and fungi as well as soil dehydrogenase activity. These effects are gradually weakened and even reverse with the increase of exposure time. The change of the substrate-induced respiration (SIR) is significantly increased under the combined stress. The data of BIOLOG are subjected to the principle component analysis (PCA) and three index models such as Shannon, Simpson and McIntosh indexes. The results also indicate that the combined application of acetochlor and copper sulphate can destroy the richness and evenness of microbial community diversity in phaeozem. The changes of carbon utilization diversity of phaeozem microbial community are also showed by the pattern of PCA.


Assuntos
Sulfato de Cobre/química , Ecossistema , Microbiologia do Solo , Solo/análise , Toluidinas/química , Actinobacteria/efeitos dos fármacos , Biodiversidade , Carbono , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , Herbicidas/química
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