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1.
Int J Biol Macromol ; 152: 314-326, 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-32109475

RESUMO

The aim of this paper was to investigate the extraction, purification and characterization of sulphated polysaccharide (BQPS) from Bellamya quadrata and its stabilization roles on atherosclerotic plaque. Firstly, crude polysaccharide (CBQP) from Bellamya quadrata was extracted by protease enzyme assisted extraction. Moreover, its optimal parameters were obtained by the response surface method as follows: the ratio of water to raw material of 24:1, enzyme dosage of 285 U/g, enzymolysis pH value of 4.7 and temperature of 67 °C. Secondly, CBQP was further purified to obtain the target polysaccharide BQPS by Q Sepharose Fast Flow and Sephacryl S-400 gel column chromatography. Then, the characterization of BQPS revealed that it possessed a total polysaccharide content of 91.88 ± 1.23%, sulfuric acid group content of 9.12 ± 1.59% and molecular weight of 91.1 kDa. BQPS was only consisted of glucose without any proteins. Finally, BQPS was confirmed to have a significant stabilizing effect on atherosclerotic plaque and its mechanism was related to the selective promotion of autophagy with the precisely right strength.

2.
Food Funct ; 10(10): 6644-6654, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31556417

RESUMO

Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear. In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation. First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks. Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared. The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner. Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria. Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.


Assuntos
Aterosclerose/prevenção & controle , Microbioma Gastrointestinal/efeitos dos fármacos , Gastrópodes/química , Polissacarídeos/administração & dosagem , Substâncias Protetoras/administração & dosagem , Animais , Apolipoproteínas E/deficiência , Apolipoproteínas E/genética , Aterosclerose/genética , Aterosclerose/metabolismo , Aterosclerose/microbiologia , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Dieta Hiperlipídica/efeitos adversos , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Polissacarídeos/química , Substâncias Protetoras/química
3.
J Spinal Cord Med ; 42(3): 371-377, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-29141514

RESUMO

OBJECTIVE: The present study was aimed to investigate the therapeutic potential of neuroglobin in the recovery of spinal cord injury. METHODS: The male albino Wistar strain rats were used as an experimental model, and adeno associated virus (AAV) was administered in the T12 section of spinal cord ten days prior to the injury. Basso Beattie Bresnahan (BBB) locomotor rating scale was used to determine the recovery of the hind limb during four weeks post-operation. Malondialdehyde (MDA), catalase and superoxide dismutase (SOD) were determined in the spinal cord tissues. Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay was carried out to determine the presence of apoptotic cells. Immunofluorescence analysis was carried out to determine the neuroglobin expression. Western blot analysis was carried out to determine the protein expressions of caspase-3, cytochrome c, bax and bcl-2 in the spinal cord tissues. RESULTS: Experimental results showed that rats were recovered from the spinal cord injury due to increased neuroglobin expression. Lipid peroxidation was reduced, whereas catalase and SOD activity were increased in the spinal cord tissues. Apoptosis and lesions were significantly reduced in the spinal cord tissues. Caspase-3, cytochrome c and bax levels were significantly reduced, whereas bcl-2 expression was reduced in the spinal cord tissues. CONCLUSION: Taking all these data together, it is suggested that the increased neuroglobin expression could improve the locomotor function.

4.
J Orthop Surg Res ; 13(1): 215, 2018 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-30157879

RESUMO

BACKGROUND: Cervical ossification of the posterior longitudinal ligament (OPLL) is a progressive disease. Posterior decompression surgery is reported to be an effective and comparatively safe procedure with few complications for treatment of patients with myelopathy caused by OPLL. However, some patients require revision surgery because of late neurological deterioration due to OPLL progression or kyphotic changes in cervical alignment. This study reports preliminary clinical results of anterior controllable antidisplacement and fusion (ACAF), a novel revision surgery after initial posterior surgery for OPLL. METHODS: From January 2017 to June 2018, ten patients with cervical OPLL who underwent ACAF revision surgery after initial posterior surgery were included in this study. The mean age was 62.1 ± 8.0 years (52-78), and the mean interval between initial posterior surgery and revision was 78.0 ± 48.2 months (5-180). The Japanese Orthopaedic Association (JOA) scales, Neck Disability Index (NDI), visual analog scale (VAS), and surgical complications were recorded. RESULTS: The mean surgery time was 179.3 ± 41.8 min (120-240), and the mean blood loss was 432.5 ± 198.3 ml (225-850). The patients were followed up for at least 12 months. The JOA scores improved from 8.7 ± 2.8 to 13.4 ± 2.4; the mean improvement rate was 59.9% ± 16.1%. Postoperative NDI and VAS scores were 13.3 ± 3.7 and 2.0 ± 1.6, respectively, and were significantly improved compared to those before the procedure (P < 0.05). Cervical lordosis improved from 3.8 ± 4.3° to 17 ± 4.6° after revision surgery. There was only one instance of cerebrospinal fluid (CSF) leakage; no instances of postoperative hematoma, C5 root palsy, or hoarseness occurred. CONCLUSIONS: The present study demonstrates that excellent postoperative outcomes can be achieved with the ACAF technique for revision treatment of OPLL. Though further study is required to confirm the conclusion, this novel technique has the potential to serve as an alternative surgical technique for revision treatment of OPLL.


Assuntos
Ossificação do Ligamento Longitudinal Posterior , Reoperação , Idoso , Descompressão Cirúrgica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ossificação do Ligamento Longitudinal Posterior/cirurgia , Osteogênese , Estudos Retrospectivos , Resultado do Tratamento
5.
Med Sci Monit ; 24: 2524-2532, 2018 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-29691361

RESUMO

BACKGROUND Salvianolic acid B (SB) is a major active phyto-component of the plant Radix Salvia miltiorrhiza, which is traditionally used to treat joint pain and arthritis. The present study examined the anti-rheumatoid arthritis efficacy of SB on collagen-induced rheumatoid arthritis (CIA) in a rat model. MATERIAL AND METHODS Forty-eight rats were divided into 4 groups: Control rats treated with saline (Group I), rats subjected to CIA induction by intradermal injection of bovine collagen II type at the tail (Group II), and rats subjected to CIA and supplemented with either 20 or 40 mg/kg of SB for 28 days (group III or IV). RESULTS Paw swelling, edema, arthritis score, thymus and spleen indexes, and neutrophil infiltration were significantly decreased (p<0.01) by treatment with 20 or 40 mg/kg of SB. The levels of inflammatory cytokines (interleukin-1ß, -6, and -17, and TNF-α) and anti-collagen II-specific immunoglobulins (IgG1 and IgG2a) were markedly decreased (p<0.01), and those of antioxidant enzymes (SOD, CAT, and GSH) were significantly increased (p<0.01) in SB-treated rats. Administration with SB (20 or 40 mg/kg) resulted in lower phosphorylated IkB-a and NF-κB p65 protein levels and markedly downregulated IκB-a expression. Furthermore, CIA rats revealed the presence of highly diffused polymorphonuclear cells (PMNs) infiltration with eroded cartilage; however, these phenomena were considerably ameliorated by SB. CONCLUSIONS SB alleviates oxidative stress and inflammation in CIA rats, thus verifying its anti-rheumatoid arthritis property.


Assuntos
Artrite Reumatoide/tratamento farmacológico , Benzofuranos/metabolismo , Benzofuranos/farmacologia , Animais , Anti-Inflamatórios/uso terapêutico , Antioxidantes/farmacologia , Antirreumáticos/uso terapêutico , Artrite Experimental/tratamento farmacológico , Artrite Reumatoide/induzido quimicamente , Artrite Reumatoide/imunologia , Benzofuranos/uso terapêutico , Modelos Animais de Doenças , Regulação para Baixo , Edema/tratamento farmacológico , Inflamação/tratamento farmacológico , Mediadores da Inflamação/metabolismo , Masculino , Inibidor de NF-kappaB alfa/metabolismo , NF-kappa B/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Ratos , Ratos Sprague-Dawley , Fator de Transcrição RelA/metabolismo
6.
Carbohydr Polym ; 176: 152-159, 2017 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-28927593

RESUMO

The purpose of this paper was to investigate the anti-inflammatory and anti-angiogenic activities of a purified polysaccharide (CCPSn) from flesh of Cipangopaludina chinensis in lipopolysaccharide (LPS) activated RAW264.7 macrophages and human umbilical vein endothelial cells (HUVECs), respectively. Anti-inflammatory results showed that CCPSn not only greatly decreased the pro-/anti-inflammatory cytokine secretion ratios, including TNF-α/IL-10, IL-6/IL-10 and IL-1ß/IL-10, but also reduced release levels of nitric oxide (NO) and prostaglandin E2 (PGE2), and suppressed expressions of cyclooxygenase2 (COX2) and inducible nitric oxide synthase (iNOS). The results from anti-angiogenic activities revealed that proliferation, migration, tube formation and vascular endothelial growth factor (VEGF) of HUVECs were significantly inhibited by CCPSn treatment. Even more remarkable was the fact that anti-inflammatory and anti-angiogenic activities of CCPSn exhibited a clear dose-response manner, and these activities were irrelevant to the cytotoxicities of CCPSn to RAW264.7 macrophages and HUVECs. These results indicated that CCPSn possessed strong anti-inflammatory and anti-angiogenic activities.


Assuntos
Inibidores da Angiogênese/farmacologia , Anti-Inflamatórios/farmacologia , Gastrópodes/química , Polissacarídeos/farmacologia , Animais , Ciclo-Oxigenase 2/metabolismo , Citocinas/metabolismo , Dinoprostona/metabolismo , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Humanos , Lipopolissacarídeos , Macrófagos/efeitos dos fármacos , Camundongos , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Células RAW 264.7 , Fator A de Crescimento do Endotélio Vascular/metabolismo
7.
Food Chem ; 217: 461-468, 2017 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-27664659

RESUMO

An efficient decoloration method for polysaccharides from the sprouts of Toona sinensis (A. Juss.) Roem (PSTS) by anion exchange macroporous resins (AEMR) was investigated in the present paper. The results suggested that D941 resin offered better decoloration efficiency than other tested resins. Based on single-factor experiments, the optimal decoloration parameters of D941 resin were obtained as follows: temperature of 45°C, sample initial concentration of 30mg/ml, pH value of 8.5, static decoloration time of 90min, dynamic decoloration processing volume of 5.5BV with the flow rate of 2BV/h. Decoloration ratio, PSTS recovery ratio and selectivity coefficient were 91.94±1.23%, 90.05±2.35% and 10.92±0.63, respectively. Most of pigment impurities were successfully removed from PSTS solutions after treated by D941 resin, and there was no significant difference in carbohydrate concentration, characteristic groups and molecular weight. Compared with H2O2 oxidation and activated carbon adsorption, this developed method is superior.


Assuntos
Resinas de Troca de Ânions/química , Meliaceae , Pigmentos Biológicos/análise , Extratos Vegetais/análise , Polissacarídeos/análise , Plântula/química , Adsorção
8.
BMC Musculoskelet Disord ; 17: 309, 2016 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-27450593

RESUMO

BACKGROUND: The results of studies investigating the association between the ladybird homeobox 1 (LBX1) gene polymorphisms and the risk of adolescent idiopathic scoliosis (AIS) are not all the same. As such, we performed a meta-analysis to estimate the association between LBX1 gene polymorphisms and AIS susceptibility. METHODS: Relevant studies published before 15 November 2015 were identified by searching PubMed, EMBASE, ISI web of knowledge, EBSCO, CNKI and CBM. The strength of relationship was assessed by using odds ratios (ORs) and 95 % confidence interval (CI). RESULTS: A total number of eight case-control studies including 10,088 cases and 24,538 controls were identified. The results showed that T allele of rs111090870 increased AIS susceptibility in Asians (T vs. C, OR = 1.22, 95 % CI: 1.16-1.29, P < 0.001), Caucasians (T vs. C, OR = 1.17, 95 % CI: 1.14-1.21, P < 0.001) and in female (T vs. C, OR = 1.21, 95 % CI: 1.17-1.25, P < 0.001). The G allele of rs678741 decreased AIS risk in female (G vs. A, OR = 0.83, 95 % CI: 0.81-0.85, P < 0.001), and the G allele of the rs625039 increased AIS susceptibility in Asians (G vs. A, OR = 1.14, 95 % CI: 1.11-1.17, P < 0.001). CONCLUSIONS: Our meta-analysis provides evidence that rs111090870, rs678741 and rs625039 polymorphisms near LBX1 gene are associated with AIS susceptibility in some populations. However, our findings are based on only a limited number of studies.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Grupo com Ancestrais do Continente Europeu/genética , Predisposição Genética para Doença , Proteínas de Homeodomínio/genética , Escoliose/genética , Fatores de Transcrição/genética , Adolescente , Fatores Etários , Alelos , Criança , Feminino , Genótipo , Humanos , Masculino , Razão de Chances , Polimorfismo de Nucleotídeo Único , Fatores de Risco , Fatores Sexuais
9.
Medicine (Baltimore) ; 95(19): e3435, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27175643

RESUMO

The blood loss during total hip arthroplasty is difficult to manage and there is no consensus about the effect of bipolar sealer used during operation. Thus, a systematic review of randomized controlled trials (RCTs) was performed to evaluate the efficacy and safety of blood loss using bipolar sealer after total hip arthroplasty (THA).Relevant literature of comparisons of bipolar sealer after THA for blood loss were searched for in Embase, PubMed, Web of Science, the Cochrane Central Register of Controlled Trials, and Google scholar from their inception to October, 2015. High-quality RCTs were selected to evaluate the need for transfusion, blood loss, and other complications. The software RevMan 5.30 was used for the meta-analysis.Six studies reporting on 6 RCTs comprising 751 patients were included. Compared with standard electrocautery, bipolar sealer was associated with lower rates of need for transfusion (relative risk [RR] = 0.60; 95% confidence interval [CI] 0.39-0.94), estimated blood loss (mean differences [MD] = -127.39; 95% CI -233.32 to -21.46; P = 0.02), and lower total blood loss (MD = -226.57; 95% CI -350.80-102.34; P = 0.0004). There is no significant difference between the hemoglobin drop, blood loss in drainage, intraoperative blood loss, Harris score, and rates of infection.The present meta-analysis indicated that bipolar sealer can decrease the need for transfusion and total blood loss; however, there is no benefit of bipolar sealer from the recovery. It is still need for samples to determine the balance between the economic cost and transfusion.


Assuntos
Artroplastia de Quadril/instrumentação , Perda Sanguínea Cirúrgica/prevenção & controle , Hemostasia Cirúrgica/instrumentação , Idoso , Artroplastia de Quadril/efeitos adversos , Transfusão de Sangue/estatística & dados numéricos , Eletrocoagulação/métodos , Feminino , Hemostasia Cirúrgica/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Hemorragia Pós-Operatória/prevenção & controle , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento
10.
J Int Med Res ; 44(4): 931-9, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27235408

RESUMO

OBJECTIVE: To investigate how the anatomy of variant atlas vertebra impacts on the strategy used to place pedicle screws used to treat atlantoaxial instability. METHODS: The study enrolled patients with cervical instability who had a posterior arch pedicle height <3.5 mm at the anchor point, a vertebral artery groove height <3.5 mm, or both. Pedicle screws were fitted according to the anatomy of the variant atlas vertebra. Patients were followed-up to evaluate accuracy of the screw placement and maintenance of cervical stability. RESULTS: A total of 28 patients were enrolled. The mean height of the atlas pedicle proximal section was >5.0 mm. For the vertebral artery groove, the height of the lateral region was significantly greater than that of the medial region. Approximately 60% of atlas vertebrae had lateral heights >3.5 mm (34 of 56). The majority of the posterior arch heights were <3.0 mm. There were no perioperative or postoperative complications observed. CONCLUSIONS: Pedicle screw placement in the lateral pedicle region is the safest and most reliable strategy to treat variant atlas pedicles.


Assuntos
Atlas Cervical/cirurgia , Parafusos Pediculares , Adolescente , Adulto , Idoso , Atlas Cervical/diagnóstico por imagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cuidados Pré-Operatórios , Tomografia Computadorizada por Raios X , Adulto Jovem
11.
Int J Biol Macromol ; 78: 324-32, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25891913

RESUMO

In the present study, characterization and cardioprotective activities in vivo of a novel purified polysaccharide (PECGp) from Endothelium corneum gigeriae Galli were investigated. The results found that PECGp had a 96 kDa molecular weight. The backbone chains were composed of α-D-Glc and α-L-Rha linked by (1→4) glycosidic bonds. (1→4, 6) linked α-D-Glc and (1→2, 4) linked α-L-Rha in backbone chains with branches consisting of (1→4) linked α-D-Glc and terminal α-D-Glc-6-COOH. The assay of cardioprotective activities demonstrated that PECGp could significantly reduce the ST-segment elevation, prevented abnormal changes of heart muscle morphology, reverse abnormal hemodynamic and hemorheological parameters, rectify disorganized superoxide dismutase, nitric oxide synthase, nitric oxide, malondialdehyde, creatine kinase, creatine kinase MB fraction and lactate dehydrogenase levels. The results suggested that PECGp could be considered as a potential candidate for developing novel cardioprotective agents.


Assuntos
Cardiotônicos/química , Cardiotônicos/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Animais , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Creatina Quinase/sangue , Creatina Quinase/metabolismo , Creatina Quinase Forma MB/sangue , Eletrocardiografia/efeitos dos fármacos , Hemodinâmica/efeitos dos fármacos , Hemorreologia , L-Lactato Desidrogenase/sangue , Masculino , Malondialdeído/sangue , Malondialdeído/metabolismo , Infarto do Miocárdio/sangue , Infarto do Miocárdio/tratamento farmacológico , Infarto do Miocárdio/metabolismo , Infarto do Miocárdio/patologia , Miocárdio/metabolismo , Espectroscopia de Prótons por Ressonância Magnética , Ratos , Superóxido Dismutase/sangue , Superóxido Dismutase/metabolismo
12.
Food Chem ; 179: 15-25, 2015 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-25722134

RESUMO

In this paper, an economical and efficient technology for the extraction of resveratrol from peanut sprouts by multi-stage countercurrent extraction (MSCE) was investigated based on the alkaline extraction and acid precipitation method (AEAP). Firstly, the MSCE equipment and operation procedures were designed. Then, the optimal parameters of MSCE were obtained by using single-factor experiments and Box-Behnken design (BBD) as follows: extraction temperature of 46.6 °C, CaO to raw material ratio of 6:100, water to raw material ratio of 8.8:1 and extraction time of 51.7 min. Finally, the performance of MSCE was compared against the single pot extraction (SPE) under optimal conditions. The results demonstrated that MSCE was a time-saving, energy-saving, and cost-saving extraction technology for manufacturing resveratrol from peanut sprouts.


Assuntos
Arachis/química , Distribuição Contracorrente/métodos , Estilbenos/isolamento & purificação , Resveratrol , Extração em Fase Sólida
13.
Carbohydr Polym ; 110: 10-7, 2014 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-24906722

RESUMO

In this study, optimization of ultrasound-assisted extraction and antioxidant activity of polysaccharides from the aerial root of Ficus microcarpa (FMPS) were investigated. The optimal conditions for extraction of FMPS were determined as followings: ultrasound power 200 W, ultrasound temperature 70°C, extraction temperature 74°C, liquid-solid ratio 35, extraction time 238 min, ultrasound time 49 min. The experimental yield of FMPS (3.44%) obtained under these conditions was well agreement with the value predicted by the model. In addition, Fourier transform-infrared spectroscopy and antioxidant activity assays revealed that FMPS were acidic polysaccharides and had strong Fe2+ chelating activity and moderate hydrogen peroxide scavenging effect. Further work on the purification, structure characterization and antioxidant activity in vivo of FMPS is in progress.


Assuntos
Antioxidantes/efeitos da radiação , Medicamentos de Ervas Chinesas/efeitos da radiação , Ficus , Raízes de Plantas , Polissacarídeos/efeitos da radiação , Som , Antioxidantes/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Componentes Aéreos da Planta , Polissacarídeos/isolamento & purificação
14.
Carbohydr Polym ; 102: 912-9, 2014 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-24507363

RESUMO

In this study, purification, preliminary characterization, immunostimulatory and hepatoprotective activities of polysaccharides from Glossaulax didyma (GDPS) were investigated. Firstly, crude GDPS was purified by chromatography of DEAE-52 and Sephadex G-100, resulting in three purified fractions of GDPS-1, GDPS-2 and GDPS-3. The three fractions were mainly composed of glucose with the average molecular weight of 161, 197 and 204 kDa, respectively. GDPS-2 was quite different from GDPS-1 or GDPS-3. It had much higher contents of protein and sulfuric radical. For immunostimulatory activities in vitro, the three fractions could significantly stimulate the proliferation of splenocytes and enhance phagocytic activity of peritoneal macrophages. For hepatoprotective activity in vivo, the administration of GDPS significantly decreased the serum levels of alanine aminotransferase, aspartate aminotransferase and tumor necrosis factor-alpha, inhibited the formation of malondialdehyde and restored the liver activities of superoxide dismutase and glutathione peroxidase in Bacillus Calmette-Guerin/lipopolysaccharide-induced liver injury mice. The results suggested GDPS should be a potent natural polymer with immunostimulatory and hepatoprotective activities.


Assuntos
Gastrópodes/química , Macrófagos Peritoneais/efeitos dos fármacos , Polissacarídeos/isolamento & purificação , Animais , Cromatografia por Troca Iônica , Fígado/efeitos dos fármacos , Fígado/fisiologia , Testes de Função Hepática , Macrófagos Peritoneais/imunologia , Peso Molecular , Fagocitose/efeitos dos fármacos , Polissacarídeos/química , Polissacarídeos/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier
15.
Food Chem ; 145: 1-7, 2014 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-24128441

RESUMO

In the present study, the separation and purification characteristics of resveratrol from extract of peanut sprouts (PSE) on 11 macroporous adsorption resins were investigated. The results showed that ADS-5 offered better adsorption and desorption capacity for resveratrol than other tested resins. From the static experiments with ADS-5 resin, we found that the experimental data fitted best to the pseudo-second-order kinetics model and Langmuir isotherm model, and the adsorption was a spontaneous and endothermic process. The separation and purification parameters of resveratrol from PSE were optimised by dynamic adsorption/desorption experiments with the column packed with ADS-5 resin. Under the optimal conditions, after one run treatment with ADS-5 resin, the content of resveratrol in the product was increased 17.9-fold from 1.32% to 23.60%, with a recovery yield of 88.33%. The results demonstrated that ADS-5 resin was a promising basis for large-scale preliminary separation and purification of resveratrol from PSE.


Assuntos
Arachis/química , Extratos Vegetais/análise , Resinas Sintéticas/química , Estilbenos/isolamento & purificação , Adsorção , Resveratrol , Estilbenos/química , Termodinâmica
16.
Zhongguo Gu Shang ; 26(6): 493-6, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24015655

RESUMO

OBJECTIVE: To explore radiographic results and clinical effects of posterior atlanto axial vertebra internal fixation in treating instability of occipitocervical. METHODS: The clinical data of 155 patients with instability of occipitocervical treated by posterior atlanto axial vertebra internal fixation were respectively analyzed from September 2005 to January 2011. There were 68 males and 87 females, ranging in age from 6 to 75 years old with an average of 45.6 years old. Of them, 53 cases were fresh odontoid fractures(Aderson type II C), 30 cases were os odontoideum, 20 cases were old odontoid fractures, 18 cases were unstable atlas fractures, 12 cases were atlanto axial rotatory dislocation, 11 cases were atlanto axial dislocation after rheumatoid arthritis, and 11 cases were basilar invagination. Radiographic results were evaluated in terms of atlas pedicle screw fixation, bone healing and bone graft fusion. Clinical effect evaluation included relief of pain in the occipital-cervical region by VAS score and JOA score. RESULTS: Totally 300 screws were set through atlas pedicle screw fixation in 150 patients. Five patients receivde hook fixation. Postoperative CT showed ideal nailing were 275 (91.7%),acceptable nailing were 14 (4.7%) and unacceptable nailing were 11 (3.6%). All patients were followed up, and the duration ranged from 16 to 40 months with an average of 25.4 months. The fresh fractures healed and 140 cases got bone graft fusion. Preoperative VAS and JOA score were respectively improved from (7.2 +/- 1.1), (7.3 +/- 2.4) to (3.2 +/- 1.1), (13.3 +/- 2.4) at the latest follow-up. CONCLUSION: Posterior atlanto axial vertebra internal fixation in treating instability of occipitocervical can effectively recover physiological curvature of cervical, provide mechanical stability, and obtain good clinical effect. For the young patients who require further activity, posterior fixation and non-fusion technology is a good choose, which can avoid bone graft.


Assuntos
Articulação Atlantoaxial/cirurgia , Atlas Cervical/cirurgia , Fixação Interna de Fraturas , Instabilidade Articular/cirurgia , Adolescente , Adulto , Idoso , Parafusos Ósseos , Criança , Feminino , Humanos , Fixadores Internos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
17.
Food Chem Toxicol ; 62: 226-30, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24007737

RESUMO

In the present study, we investigated the preliminary characterization, both in vitro and in vivo immunostimulatory activity of polysaccharides from Glossaulax didyma (GPS). The results of chemical and gas chromatography analysis indicated that GPS was mainly composed of glucose with high contents of uronic acid and sulfate. For immunostimulatory activity in vitro, GPS could stimulate the proliferation of splenocytes and enhance neutral red phagocytosis, TNF-α production level and acid phosphatase activity in mouse peritoneal macrophages. For immunostimulatory activity in vivo, the administration of GPS could significantly increase the level of serum hemolysin in cyclophosphamide-treated mice. The results provided evidences that GPS possessed significant immunostimulatory activity and might be one of important active substances responsible for the immunostimulatory effect of G. didyma.


Assuntos
Adjuvantes Imunológicos/farmacologia , Polissacarídeos/farmacologia , Baço/efeitos dos fármacos , Fosfatase Ácida/metabolismo , Adjuvantes Imunológicos/química , Animais , Proliferação de Células/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos/métodos , Feminino , Gastrópodes/química , Hemolíticos/farmacologia , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/metabolismo , Camundongos Endogâmicos ICR , Monossacarídeos/análise , Fagocitose/efeitos dos fármacos , Polissacarídeos/análise , Polissacarídeos/química , Baço/citologia , Fator de Necrose Tumoral alfa/metabolismo
18.
Carbohydr Polym ; 98(1): 217-23, 2013 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-23987338

RESUMO

In this study, we investigated the purification, preliminary characterization and immunostimulatory activity in vivo of polysaccharide from Cipangopaludina chinensis (CCPS). Firstly, crude CCPS was prepared by hot water extraction. And the crude CCPS was sequentially purified by chromatography of DEAE-52 and Sephadex G-100, resulting in two purified fractions of CCPS-1 and CCPS-2. We found the two fractions were homogeneous heteropolysaccharides mainly composed of rhamnose and glucose with the average molecular weight of 226 and 235 kDa, respectively. CCPS-2 was quite different from CCPS-1. It had much higher content of uronic acid and sulfuric radical. For immunostimulatory activity in vivo, crude CCPS could significantly increase the thymus and spleen indices, enhance the macrophage function, and increase the level of serum hemolysin in cyclophosphamide-treated mice, suggesting CCPS had a potent immunostimulatory activity and could be explored as a potential natural immunomodulatory agent.


Assuntos
Adjuvantes Imunológicos/isolamento & purificação , Adjuvantes Imunológicos/farmacologia , Gastrópodes/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Adjuvantes Imunológicos/química , Animais , Ciclofosfamida/farmacologia , Proteínas Hemolisinas/sangue , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Masculino , Camundongos , Peso Molecular , Polissacarídeos/química , Baço/efeitos dos fármacos , Baço/imunologia , Timo/efeitos dos fármacos , Timo/imunologia
19.
Eur J Orthop Surg Traumatol ; 23 Suppl 1: S41-6, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23736870

RESUMO

STUDY DESIGN AND OBJECTIVE: This study performs a systematic review to compare the functional outcomes and complications between the dynamic cervical plate and static cervical plate in patients with the anterior cervical discectomy and fusion (ACDF). The common static cervical plates have been widely used in the ACDF. It can successfully increase the fusion rate and decrease the surgery failure. Recently, the dynamic plate has been identified as another safe and efficient option for the better fusion rate by promoting load sharing across the construct. However, the proposed benefits have been largely theoretical, and there is considerable controversy as to which plate is a better option for reconstruction after ACDF. METHODS: We searched the Cochrane Library, EMBASE, PubMed, and CBM to identify the clinical studies regarding the comparison of dynamic cervical plate with fixed cervical plate in the ACDF. Reports not available in English were excluded. The quality of the included studies was critically assessed, and the data analysis was performed by the Cochrane Collaboration's RevMan 4.2. We defined statistical significance as a P value <0.05. RESULTS: Five studies were included in this systematic review. In the final analysis, there were 172 patients in the dynamic cervical plate and 143 in the static cervical group. Four studies compared the clinical and radiographic outcomes between the two plate groups in the one-level or two-level fusion segmentation patients, while one studied the patients with the multiple levels. The similar clinical outcomes between the two cervical plate systems were reported in two studies. However, another study suggested that a better clinical outcome was found in the dynamic plate group for the multiple-level fusion patients, although the similar clinical outcome was found in the one-level fusion patients. The two RCT studies with the same clinical data reported that four patients in the static group developed hardware complications, while there was no implant complication in the dynamic group. CONCLUSION: The clinical outcome was similar in ACDF for one-level fusion patients, although the hardware failure rate was higher in ACDF with static plates.


Assuntos
Placas Ósseas , Discotomia/efeitos adversos , Degeneração do Disco Intervertebral , Complicações Pós-Operatórias , Fusão Vertebral/efeitos adversos , Espondilose , Vértebras Cervicais/cirurgia , Pesquisa Comparativa da Efetividade , Discotomia/instrumentação , Discotomia/métodos , Humanos , Degeneração do Disco Intervertebral/fisiopatologia , Degeneração do Disco Intervertebral/cirurgia , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/fisiopatologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Recuperação de Função Fisiológica , Fusão Vertebral/instrumentação , Fusão Vertebral/métodos , Espondilose/fisiopatologia , Espondilose/cirurgia , Resultado do Tratamento
20.
Zhongguo Gu Shang ; 24(9): 729-31, 2011 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-22007578

RESUMO

OBJECTIVE: To investigate therapeutic effects of arthroscopic treatment for acute patellar dislocation. METHODS: From May 2002 to March 2009, 36 patients with acute patellar dislocation were treated with arthroscopy, including taking out of the free cartilage chips, relaxing lateral retinaculum and tight suturing medial retinaculum. Among the patients, 6 patients were males and 30 patients were females, ranging in age from 12 to 30 years (averaged, 20.5 years). Fifteen patients had the dislocations on the left an 21 patients had the dislocations on the right. The development of condyles of femur and Q trianglel were detected by preoperative radiology. The Lysholm criteria system was used to evaluate the clinical effects. RESULTS: All the patients were followed up, and the duration ranged from 13 to 60 months (averaged, 42 months). All the patients had no complications such as infection, and the knee range of motion recovered to normal without pain. The Lysholm score improved from preoperative (28.9 +/- 2.5) to postoperative (95.1 +/- 8.4). All the patients had no redislocation. CONCLUSION: Acute dislocation of the patella is an potential devastating injury. The arthroscopic treatment for acute patellar dislocation has advantages such as less trauma, faster recovery and more reliable.


Assuntos
Artroscopia/métodos , Luxação Patelar/cirurgia , Adolescente , Adulto , Criança , Feminino , Humanos , Articulação do Joelho/cirurgia , Masculino , Adulto Jovem
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