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1.
Exp Biol Med (Maywood) ; : 1535370220961013, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33023331

RESUMO

IMPACT STATEMENT: We investigated that CCAT1 expression was elevated in NSCLC and CCAT1 deletion was identified to inhibit cell carcinogenic phenotypes in NSCLC cells via miR-216a-5p/RAP2B axis, which reveals a novel pathway underlying progression in NSCLC cells and providing potential targets for NSCLC treatment.

2.
BMC Microbiol ; 20(1): 297, 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33008292

RESUMO

BACKGROUND: Heterotrichous ciliates are common members of microeukaryote communities which play important roles in both the transfer of material and the flow of energy in aquatic food webs. This group has been known for over two centuries due to their large body size and cosmopolitan distribution. Nevertheless, species identification and phylogenetic relationships of heterotrichs remain challenging due to the lack of accurate morphological information and insufficient molecular data. RESULTS: The morphology and phylogeny of two heterotrichous ciliates, namely Gruberia foissneri spec. nov. and Linostomella vorticella (Ehrenberg, 1833) Aescht in Foissner et al., 1999, were studied using rigorous methods (living morphology, stained preparations, and small subunit rDNA sequence data). Gruberia foissneri spec. nov. is morphologically very similar to G. uninucleata Kahl, 1932, however, it can be distinguished from the latter by having more ciliary rows (about 32 vs. about 20) and macronuclear shape (sausage-shaped vs. ellipsoid). Based on a combination of previous and present studies, an improved diagnosis of L. vorticella is supplied and several taxonomic anomalies are clarified. In addition, phylogenetic analyses based on SSU rDNA sequence data support the generic assignment of these two species. CONCLUSIONS: Modern ciliate taxonomy should be performed by means of detailed living observation, stained preparations and molecular information. For those species that have been reported in previous studies, it is necessary to provide as much useful information as possible using state-of-the-art methods in order to resolve taxonomic anomalies.

3.
Sci Total Environ ; : 142275, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33077214

RESUMO

This study examines the sensitivity of daily PM2.5 to NOx emissions and meteorology using in situ observations from main cities of North China (NC). NC cities are divided into low-, medium-, and high-emission groups by the ranking of their 4-year mean NO2. For each emission group, daily NO2 levels are used to divide the days into good-, medium-, and bad-meteorological conditions. Regardless of their emission levels, all cities reveal significant decreases (96%-172%) in daily PM2.5 levels from bad to good meteorological conditions. The largest difference in PM2.5 concentrations between the emissions groups is found under bad meteorological conditions, with 56% higher PM2.5 in high-emission cities than low-emission cities, indicating PM2.5 under bad meteorological conditions has the largest sensitivity to emissions. The high-emission, bad-meteorology group saw a 24% decrease in mean daily PM2.5 levels from 2017, a high-emission year, to 2019, a low-emission year. However, under good meteorological conditions, the high-emissions group shows an increase of 8.8 µg/m3 in mean daily PM2.5 from 2017 to 2019 with a 2.6% increase in the possibility of high PM2.5. These results suggest the current emission reduction measures are more effective in controlling PM2.5 in high-emission cities under bad meteorological conditions than under other meteorological conditions.

5.
PLoS One ; 15(10): e0240125, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33035227

RESUMO

Public value is gaining prominence from both academics and politicians with regards to China's rural development. However, rural planning authorities and practitioners showed limited confidence on public, which manifests as few public perceptions were integrated into the planning documents. This study explores the potential role of residents' experiences in illustrating local economic development within the context of coastal villages in which economic and industries are rapidly transforming. Two case studies from within the locale of the Greater Hangzhou Bay Rim Area are used in this article to examine the gap between residents' experiences and the actual economic development that has occurred. The main findings suggest that rural residents can directly reflect upon both current and historic trends of local economic development. Moreover, household income satisfaction (HIS) is a comprehensive notion of residents' experiences, and indicates social and economic sustainability of industrial transformation, or "thriving business", that have been highlighted in coastal villages. Public experiences could therefore act as a valid and accessible evidence for planners in rural economic planning in China and other developing countries.

6.
Biochim Biophys Acta Gen Subj ; 1865(1): 129756, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33010351

RESUMO

BACKGROUND: Crotonase superfamily members exhibit great catalytic diversity towards various acyl-CoA substrates. A common CoA moiety binding pattern is usually observed in this family, understanding the substrate-binding mechanism would facilitate the rational engineering of crotonases for improved properties. METHODS: We applied X-ray crystallography to investigate a putative enoyl-CoA hydratase/isomerase OdaA in Pseudomonas aeruginosa. Thermal shift assay (TSA) were performed to explore the binding of OdaA with CoA thioester substrates. Furthermore, we performed molecular dynamics (MD) simulations to elucidate the dynamics of its CoA-binding site. RESULTS: We solved the crystal structures of the apo and CoA-bound OdaA. Thermal shift assay (TSA) showed that CoA thioester substrates bind to OdaA with a different degree. MD simulations demonstrated that the C-terminal alpha helix underwent a structural transition and a hinge region would associate with this conformational change. CONCLUSIONS: TSA in combination with MD simulations elucidate that the dynamics of C-terminal alpha helix in CoA-binding, and a hinge region play an important role in conformational change. GENERAL SIGNIFICANCE: Those results help to extend our knowledge about the nature of crotonases and would be informative for future mechanistic studies and industry applications.

7.
Turk J Gastroenterol ; 31(7): 489-496, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32897221

RESUMO

BACKGROUND/AIMS: This study aimed to investigate the differences and relevance of various common duodenal diseases in different parts in the aspects of age, gender, helicobacter pylori (H. pylori) infection, application of nonsteroidal anti-inflammatory drugs (NSAIDs), smoking, or alcohol consumption. MATERIALS AND METHODS: The medical records of various duodenal diseases were collected and tested for difference using the χ2 test or the Fisher exact probability method. RESULTS: 1) The proportions of duodenal ulcer (DU), inflammation, and duodenal bulb diseases in the adult group (A) (47.98%, 36.70%, and 66.63%) were higher than those in the elderly group (E) (41.38%, 29.83%, and 56.82%), but the proportions of duodenal diverticulum (DD) and tumor diseases in the descending and ascending segments (2.95%, 1.43%, 9.14%, and 0.14%) were lower than those in group E (13.73%, 3.69%, 19.41%, and 0.76%) (p<0.001). 2) The positive rate of H. pylori (63.64%) in the duodenal bulb diseases was higher than that in the bulb-descending segment (53.75%), but the application rate of NSAIDs (16.44%) in the duodenal bulb-descending diseases was lower than that in the descending segment (24.81%) (p<0.001). CONCLUSION: 1) DU, inflammation, and duodenal bulb diseases are common in adults, but DD and tumor diseases in the descending and ascending segments are more common in the elderly. 2) Compared with the duodenal bulb-descending diseases, the application of NSAIDs has greater impact on the diseases in the descending segment, and the rate of H. pylori infection is higher in duodenal bulb diseases.

8.
Water Sci Technol ; 82(4): 615-626, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32970615

RESUMO

Ammonium sulfate double salt crystals (ASDSCs) are formed during the electrolytic production of manganese. Typically, the large volume of ASDSCs accumulates in the open air, and this leads to serious environmental pollution and wastage of resources. In this study, we developed a new double-membrane three-chamber electrolysis method. In this method, ASDSCs were dissolved in water and then pretreated stepwise to precipitate manganese(II) carbonate and magnesium carbonate. These precipitates were filtered and the filtrate (mainly ammonium sulfate) was subjected to double-membrane three-chamber electrodecomposition to produce sulfuric acid and ammonia. Further investigations showed that under the optimal conditions of current density of 250 A/m2, electrolysis time of 18 h, and temperature of 40 °C, the decomposition rate of ammonium sulfate reached as high as 96.15%. Thus, using this method, ASDSCs can be completely decomposed, which resolves the problem of environmental pollution and provides certain economic benefits to enterprises.


Assuntos
Compostos de Amônio , Manganês , Amônia , Sulfato de Amônio , Eletrólise
9.
Environ Health Perspect ; 128(9): 97007, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32997523

RESUMO

BACKGROUND: Animal studies suggest that pesticide exposure elicits endocrine changes, increases embryo implantation failure, and decreases litter size. However, only a few epidemiological studies have evaluated the effects of pesticides on the outcomes of in vitro fertilization (IVF) pregnancies. OBJECTIVES: This study examined the associations between preconception organophosphate pesticides (OP) exposure and pregnancy outcomes among women undergoing IVF in a Chinese population. METHODS: This study included 522 women with infertility who underwent IVF. Women were recruited from a prospective study, the China National Birth Cohort (CNBC), from Shanghai, China, between July 2017 and December 2018. Demographic and clinical information were collected from medical records and through questionnaires. Preconception exposure to OP was assessed by measuring six nonspecific dialkylphosphate (DAP) metabolites [diethylthiophosphate (DETP), diethylphosphate (DEP), diethyldithiophosphate (DEDTP), dimethylthiophosphate (DMTP), dimethylphosphate (DMP), dimethyldithiophosphate (DMDTP)] in urine samples collected at recruitment. Generalized estimating equation (GEE) models were used to evaluate the associations between OP and pregnancy outcomes. RESULTS: Compared with women in the lowest quartile (Q1) of individual DEP and Σ4DAP (the sum of DMP, DMTP, DEP, and DETP), women in the highest quartile (Q4) had lower odds of successful implantation, clinical pregnancy, and live birth, and most of the negative trends were significant (p-trends<0.05). There were no significant associations between urinary DAP concentrations and early IVF outcomes, including total and mature oocyte counts, best embryo quality, fertilization, E2 trigger levels, and endometrial wall thickness. CONCLUSION: Preconception OP exposure was inversely associated with successful implantation, clinical pregnancy, and live birth in women who underwent IVF. https://doi.org/10.1289/EHP7076.

10.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 38(4): 475-478, 2020 Aug 01.
Artigo em Chinês | MEDLINE | ID: mdl-32865372

RESUMO

This study aims to apply a new expert system to design removable partial denture (RPD) framework. The RPD design is completed in three steps, namely, "selecting missing teeth", "selecting abutment condition", and "selecting personalized clasp". The system can help auxiliary dentists develop personalized treatment plans to reduce their clinical workload. It can also generate a dental preparation guideline for clinical preparation, which can prevent tooth preparation mistakes. By generating the standard electronic drawings of the framework design, the system can reduce the inconvenience caused by manual drawing, thereby facilitating dentist-technician communication and reducing the rate of remade.


Assuntos
Prótese Parcial Removível , Dente , Dente Suporte , Planejamento de Dentadura , Sistemas Especialistas
11.
Biomed Mater Eng ; 31(4): 253-267, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32894237

RESUMO

BACKGROUND: Pulmonary micronodules account for 80% of all lung nodules. Generally, pulmonary micronodules in the early stages can be detected on thoracic computed tomography (CT) scans. Early diagnosis is crucial for improving the patient's survival rate. OBJECTIVE: This paper aims to estimate the malignancy risk of pulmonary micronodules and potentially improve the survival rate. METHODS: We extract 3D features of the CT images to obtain richer characteristics. Because superior performance can be achieved by having deep layers, we apply a 3D residual network (3D-ResNet) to classify the pulmonary micronodule. We construct a framework by using three parallel ResNets whose inputs are CT images in different regions of interest, i.e., the multiview of the image. To further evaluate the applicability of the framework, we make a five-category classification and achieve good performance. RESULTS: By fusing different characteristics from three views, we achieve the area under the receiver operating characteristic curve (AUC) of 0.9681. Based on the results of the experiments, our 3D-ResNet has a better performance than 3D-VGG and 3D-Inception in terms of precision (the increase rates are 13.7% and 7.4%), AUC (the increase rates are 15.8% and 5.3%), and accuracy (the increase rates are 14.3% and 4.5%). Meanwhile, the recall performance is close to that of the 3D-Inception network. CONCLUSION: Overall, the framework we propose has applicability and feasibility in pulmonary micronodule classification.

12.
Bioresour Technol ; 319: 124106, 2020 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-32949830

RESUMO

The individual and combined impacts of copper ion (Cu2+) and oxytetracycline (OTC) on anaerobic ammonium oxidation (anammox) performance and its self-recovery process were examined. Experimental results showed that the anammox performance and activity of anammox bacteria were inhibited by 1.0 mg L-1 OTC, Cu2+ and OTC + Cu2+, and both single and combined inhibitions were reversible. The abundance of functional genes and parts of antibiotic resistance genes (ARGs) were positively related to the dominant bacterium Ca. Kuenenia, implying that the recovery of the performance was associated with the progressive induction of potentially resistant species after inhibition. The above outcomes illustrated that anammox bacteria were stressed by metals and antibiotics, but they still could remove nitrogen at a rate higher than 20.6 ± 0.8 kg N m-3 d-1, providing guidance for engineering applications of anammox processes.

13.
J Therm Biol ; 92: 102679, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32888575

RESUMO

Temperature is an important abiotic environmental factor, and is responsible for various kinds of behavioral and physiological changes in living organisms. Induced heat shock is associated with feeding behaviour, reproduction and reactive oxygen species (ROS) generation that causes oxidative damage. In this experiment, we examined the lethal and sublethal effects of heat shock on reproduction, feeding behaviour and antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and peroxidases (POD) in P. solenopsis. Results showed that males were highly susceptible to heat shock treatments than females, as LTemp50 values were 43.8 °C for males and 45.11 °C for females. Heat shock events non-significantly affected the fecundity in female only treated adults and significantly affected the both sexes heat treated adults, it increased the xylem feeding duration, percentage of xylem feeding adults and reduce the phloem feeding duration and percentage of phloem feeding adults. Similarly it alter the antioxidant enzymes activities, an increase of CAT, SOD and POD activities were noticed in response to highest intensity of heat shock while a reduction of CAT and SOD activity were noticed in response to lowest intensity of heat shock compared to control (30 °C). These results suggest that heat shock may result in loss of body water and induce oxidative stress in P. solenopsis. However, antioxidant enzymes play a significant role in overcoming the oxidative damage.

14.
Environ Res ; 192: 110206, 2020 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-32956658

RESUMO

Effective river water quality management and planning is a complex issue challenged by various complexities and uncertainties. A simulation-based interval chance-constrained quadratic programming (ICCQP) model is developed for the seasonal planning of water quality management (WQM) under various uncertainties. The proposed model incorporates interval quadratic programming, chance-constrained programming, and a seasonal water quality simulation model within a general framework for WQM. Uncertainties associated with the objective and the coefficients in the left-hand sides of the constraints are tackled as intervals. Meanwhile, parameter uncertainties on the right-hand sides are characterized using probability distributions. Nonlinearities in the cost function are reflected by quadratic programming. A multi-segment water quality model is used to simulate the dynamic interactions between wastewater discharges and river water quality. The proposed ICCQP-WQM model is applied in a real case study for the control of total phosphorus (TP) in the central Grand River in Ontario, Canada. The results demonstrate that the proposed model is able to incorporate uncertainties expressed as intervals and probability information into an optimization framework and provide interval solutions. Thus, different cost-effective schemes for seasonal WQM could be generated. The results show the Kitchener wastewater treatment plant (WWTP) affects the value of the objective function more than the other WWTPs in the study area. It is also found that the Kitchener WWTP's cost accounts for the highest proportion (approximately 35.1-37.9%) of the total annual cost, which implies the control of TP at the Kitchener plant is the most important to the system. Moreover, river water TP standards in spring and autumn are usually difficult to meet, indicating different TP control strategies are needed in these two seasons. The generated results are valuable for local decision makers to generate TP control strategies, and also to identify optimized solutions under various uncertainties. The proposed ICCQP-WQM model can be extended to other watersheds to support effective water quality management and planning.

15.
Anal Cell Pathol (Amst) ; 2020: 2563208, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32802732

RESUMO

Background: JMJD2B has been reported to be implicated in malignant tumors. This study is aimed at exploring the expression and prognostic significance of JMJD2B in osteosarcoma and its association with hypoxia-inducible factor 1 (HIF1). Methods: The histopathological and clinical characteristics were retrospectively reviewed from 53 osteosarcoma patients. JMJD2B and HIF1 were examined by immunohistochemical staining of paraffin-embedded osteosarcoma samples, and their association with clinical characteristics was examined by Spearman's test. Overall survival was examined by Kaplan-Meier analysis, and prognostic factors were identified by univariate and multivariate regression analyses. Results: JMJD2B and HIF1 expression levels were both significantly associated with Enneking stage, distant metastasis, and neoadjuvant chemotherapy, and the JMJD2B and HIF1 expressions were positively correlated (p < 0.001, R = 0.752). In addition, univariate analysis showed that the expression of both JMJD2B and HIF1 was significantly associated with overall survival, but multivariate analysis showed that only JMJD2B expression was significantly associated with overall survival in osteosarcoma patients. Conclusions: JMJD2B and HIF1 expression levels show significant correlation with osteosarcoma progression, and JMJD2B could predict poor prognosis of osteosarcoma patients.

16.
Environ Pollut ; 265(Pt B): 115105, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32806467

RESUMO

Sulfate (SO42-) contamination in groundwater and surface water is an environmental problem of widespread concern. In this study, we combined stable isotope analyses of SO42- (δ34S and δ18O) and water (δ2H and δ18O) with a Bayesian mixing model (SIAR), for the first time, to identify sources and transformation of SO42- in an area of northern China with multiple potential sources of pollution. The overall values of δ34S and δ18O-SO42- ranged from 1.3‰ to 16.3‰ and -3.8‰-8.8‰ in groundwater, and from -1.1‰ to 9.3‰ and 2.7‰-9.2‰ in surface waters, respectively. Analyses of SO42- isotopes and water chemistry indicated that SO42- in groundwater and surface water mainly originated from mixing of oxidation of sulfate, sewage, chemical fertilizers, dissolution of evaporite and precipitation. There was no significant correlation between δ34S and δ18O and SO42- concentration in groundwater, indicating that bacterial sulfate reduction did not affect the SO42- isotopic composition. SIAR model showed the main sources of SO42- in groundwater and surface water comprised oxidation of sulfide minerals and sewage. In groundwater, oxidation of sulfide minerals and sewage accounted for 37.5-44.5% and 35.5-42.7% of SO42-, respectively. In regard to surface waters, the contribution of oxidation of sulfide minerals to SO42- was higher in the wet season (31.8 ± 9.9%) than in the intermediate (22.4 ± 7.8%) and dry (20.9 ± 8.2%) seasons, but the contribution proportion of sewage was slightly lower in the wet season (19.9 ± 8.5%) than in the intermediate (23.8 ± 8.7%) and dry (24.2 ± 8.5%) seasons. This study indicates that it is necessary for local government to improve the treatment infrastructure for domestic sewage and optimize methods of agricultural fertilization and irrigation to prevent SO42- contamination of groundwater and surface water.


Assuntos
Monitoramento Ambiental , Poluentes Químicos da Água/análise , Teorema de Bayes , China , Nitratos/análise , Isótopos de Nitrogênio/análise , Sulfatos
17.
Oral Dis ; 2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32815656

RESUMO

OBJECTIVE: To investigate neuroinflammation under different periodontal status MATERIALS AND METHODS: Experimental periodontitis was induced by molar ligation (Lig group) or periodontal injection of lipopolysaccharide (LPS, Lps group). Periodontal status was assessed by alveolar bone resorption and periodontal inflammation. Micro-computed tomography and hematoxylin-eosin staining were performed to assess alveolar bone resorption and periodontal inflammation, respectively. Neuroinflammation was assessed by glial cell proliferation and proinflammatory factor expression. Microgliosis was determined by immunofluorescence. Astrogliosis was determined by immunohistochemistry. Expressions of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-1ß were assessed by enzyme-linked immunosorbent assay. RESULTS: Microgliosis and astrogliosis in the Lig group were notable with molar ligation for 2 weeks and 4 weeks (P < 0.05), but were only slightly different similar from the control group by week 12. Microgliosis and astrogliosis in the Lps group were significant with LPS injection for 4 and 8 weeks (P < 0.05). The groups displayed a positive correlation between the degree of periodontal inflammation and the number of glial cells (P < 0.05). Expressions of IL-1ß and TNF-α in the Lps group were significantly increased with LPS injection for 8 weeks (P < 0.05). In the Lig group, only TNF-α was highly expressed with molar ligation for 12 weeks (P < 0.05). CONCLUSION: Both models demonstrated that the inflammatory response in the hippocampus of mice can change during periodontitis depending on the periodontal inflammation status.

18.
J Cell Physiol ; 2020 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-32841373

RESUMO

The amino acid sequence enriched with proline (P), glutamic acid (E), serine (S), and threonine (T) (PEST) is a signal-transducing agent providing unique features to its substrate nuclear proteins (PEST-NPs). The PEST motif is responsible for particular posttranslational modifications (PTMs). These PTMs impart distinct properties to PEST-NPs that are responsible for their activation/inhibition, intracellular localization, and stability/degradation. PEST-NPs participate in cancer metabolism, immunity, and protein transcription as oncogenes or as tumor suppressors. Gene-based therapeutics are getting the attention of researchers because of their cell specificity. PEST-NPs are good targets to explore as cancer therapeutics. Insights into PTMs of PEST-NPs demonstrate that these proteins not only interact with each other but also recruit other proteins to/from their active site to promote/inhibit tumors. Thus, the role of PEST-NPs in cancer biology is multivariate. It is hard to obtain therapeutic objectives with single gene therapy. An especially designed combination gene therapy might be a promising strategy in cancer treatment. This review highlights the multifaceted behavior of PEST-NPs in cancer biology. We have summarized a number of studies to address the influence of structure and PEST-mediated PTMs on activation, localization, stability, and protein-protein interactions of PEST-NPs. We also recommend researchers to adopt a pragmatic approach in gene-based cancer therapy.

19.
J Neurointerv Surg ; 2020 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-32848021

RESUMO

BACKGROUND: Brain arteriovenous malformation (BAVM) is a main cause of cerebral hemorrhage and hemorrhagic stroke in adolescents. Morphologically, a BAVM is an abnormal connection between cerebrovascular arteries and veins. The genetic etiology of BAVMs has not been fully elucidated. In this study, we aim to investigate potential recessive genetic variants in BAVMs by interrogation of rare compound heterozygous variants. METHODS: We performed whole exome sequencing (WES) on 112 BAVM trios and analyzed the data for rare and deleterious compound heterozygous mutations associated with the disease. RESULTS: We identified 16 genes with compound heterozygous variants that were recurrent in more than one trio. Two genes (LRP2, MUC5B) were recurrently mutated in three trios. LRP2 has been previously associated with BAVM pathogenesis. Fourteen genes (MYLK, HSPG2, PEAK1, PIEZO1, PRUNE2, DNAH14, DNAH5, FCGBP, HERC2, HMCN1, MYH1, NHSL1, PLEC, RP1L1) were recurrently mutated in two trios, and five of these genes (MYLK, HSPG2, PEAK1, PIEZO1, PRUNE2) have been reported to play a role in angiogenesis or vascular diseases. Additionally, abnormal expression of the MYLK protein is related to spinal arteriovenous malformations. CONCLUSION: Our study indicates that rare recessive compound heterozygous variants may underlie cases of BAVM. These findings improve our understanding of BAVM pathology and indicate genes for functional validation.

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