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1.
Bioresour Technol ; 295: 122255, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31639626

RESUMO

To investigate the feasibility of vegetal gallic acid (GA) improving silage quality, fermentation parameter, nitrogen distribution and bacterial community of mulberry leaves and stylo ensiled with 1% and 2% GA were analyzed after 60-d fermentation. The results showed that GA addition decreased dry matter loss (6.08% vs 5.35%, 17.79% vs 11.56% in mulberry leaves and style silage, respectively), pH (6.51 vs 5.98, 5.55 vs 4.57), butyric acid (0.41% and 0.83% DM, undetected in GA groups) and ammonia-N (0.71% vs 0.19%, 1.46% vs 0.29% TN) contents and increased lactic acid (2.27% vs 6.68%, 0.91% vs 1.91% DM) and acetic acid (1.68% vs 3.20%, 0.97% vs 2.02% DM) contents. Meanwhile, the relative abundance of Clostridium or Enterobacter was decreased, and that of lactate-producing bacteria was increased in mulberry leaves and stylo silage. In conclusion, GA could be used as a green additive to improve fermentation quality and protein preservation during ensiling.


Assuntos
Morus , Silagem , Bactérias , Fermentação , Ácido Gálico , Nitrogênio , Folhas de Planta
2.
Bioresour Technol ; 295: 122268, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31675519

RESUMO

To find a comprehensive way to enhance the utilizability of steam-exploded hippophae, calcium oxide (CaO) preimpregnation, cellulase-added storage and saccharification with addition of Tween 20 were investigated in this study. Both CaO preimpregnation and cellulase addition promoted the ensiling fermentation of anaerobically stored steam-exploded hippophae indicated by lower cellulose proportion and higher organic acids content, but led to the decrease of saccharification yield by 11.83% and 46.77-51.22%, respectively. When taking into account of organic acids being utilizable energy source, storing with addition of cellulase enhanced the utilizability of the materials in whole. Moreover, the addition of Tween 20 enhanced saccharification yield of the steam-exploded hippophae by 26.69-45.25%. Additionally, FTIR and XRD spectra clearly illustrated the structural alteration during storage. It is concluded that storing with addition of cellulase and hydrolyzing with addition of Tween 20 can enhance the utilizability of steam-exploded hippophae.


Assuntos
Celulase , Hippophae , Compostos de Cálcio , Hidrólise , Óxidos , Polissorbatos , Vapor
3.
J Immunother ; 43(1): 16-28, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31574023

RESUMO

Treatment with chimeric antigen receptor-modified T cell (CAR-T) has demonstrated promising therapeutic efficacy in hematologic malignancies. However, the therapeutic efficacy is still very limited for solid tumors. An immunosuppressive microenvironment is one of the main reasons for the limited efficacy. Some chemotherapeutic agents exhibit immune microenvironment modulation. Therefore, combination with chemotherapeutic agents may be one of the promising strategies to enhance the therapeutic efficacy of CAR-T against solid tumors. Sunitinib modulates the antitumor immune response by improving T-cell infiltration and function while reducing immunosuppressive factors. The authors constructed a second-generation CAR targeting human renal cell carcinoma (RCC)-specific antigen carbonic anhydrase IX (CAIX) with the costimulatory domain of 4-1BB. The results of cytokine releasing and cell killing assays showed that the CAIX-CAR-T cells have specific effector functions against CAIX renal cancer cells in vitro. Combination therapy with CAIX-CAR-T and sunitinib showed synergistic efficacy against a mouse lung metastasis model of human RCC. CAIX-CAR-T cells in the mice of the combination therapy group showed stronger proliferation and tumor infiltration than that in the mice of the CAIX-CAR-T monotherapy group. The possible mechanisms of the synergistic efficacy are: (1) sunitinib caused upregulation of CAIX in tumor cells; (2) sunitinib decreased frequency of myeloid-derived suppressor cells in the tumor microenvironment. Our study supplied an innovative immunotherapeutic approach whereby combining CAIX-CAR-T with sunitinib induces a potent antitumor response in an experimental model of metastatic RCC. The combination strategy should be considered as a potential approach to augment adoptive CAR-T cell immunotherapy.

4.
Mikrochim Acta ; 186(12): 860, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31786663

RESUMO

An aptasensor is described for the electrochemical determination of bisphenol A (BPA). Gold-coated multiwalled carbon nanotubes (Au/MWCNTs) and a single-stranded DNA-dye complex are used as a double signal-amplification system. The BPA-binding aptamer was assembled on a disposable electrode modified with Au/MWCNTs. Methylene blue (MB) was then intercalated into the immobilized aptamer with an approximately molecular ratio of 4 to form a complex. Upon interaction with BPA, the immobilized aptamer underwent a conformational change. This causes the intercalated MB to be released from the complex into solution. As a result, the electrochemical signal of the intercalated MB, typically measured using square wave voltammetry at a potential of -0.20 V (vs. Ag/AgCl (saturated KCl)) decreases. The fabrication of the aptasensor was characterized by the scanning electron microscopy, atomic force microscopy, and electrochemical techniques. Under optimal experimental conditions, the current drops linearly with the logarithm of BPA concentrations over the range from 10 fM to 1 nM, and the limit of detection is 8 fM. The assay was applied to the determination of BPA in plastic drinking bottles, tap water, and milk. Graphical AbstractSchematic illustration of fabricating the aptasensor for bisphenol A (BPA) based on double signal amplification via gold-coated multiwalled carbon nanotubes (Au/MWCNT) and an aptamer-dye complex. PET: poly(ethylene terephthalate).

5.
Biomolecules ; 9(12)2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31795133

RESUMO

Rheumatoid arthritis (RA) is a known chronic autoimmune disease can cause joint deformity and even loss of joint function. Fibroblast-like synoviocytes (FLS), one of the main cell types in synovial tissues of RA patients, are key effector cells in the development of RA and are considered as promising therapeutic targets for treating RA. Herbal medicines are precious resources for finding novel agents for treating various diseases including RA. It is reported that induction of apoptosis in FLS is an important mechanism for the herbal medicines to treat RA. Consequently, this paper reviewed the current available references on pro-apoptotic effects of herbal medicines on FLS and summarized the related possible signal pathways. Taken together, the main related signal pathways are concluded as death receptors mediated apoptotic pathway, mitochondrial dependent apoptotic pathway, NF-κB mediated apoptotic pathways, mitogen-activated protein kinase (MAPK) mediated apoptotic pathway, endoplasmic reticulum stress (ERS) mediated apoptotic pathway, PI3K-Akt mediated apoptotic pathway, and other reported pathways such as janus kinase/signal transducers and activators of transcription (JAK-STAT) signal pathway. Understanding the apoptosis induction pathways in FLS of these herbal medicines will not only help clear molecular mechanisms of herbal medicines for treating RA but also be beneficial for finding novel candidate therapeutic drugs from natural herbal medicines. Thus, we expect the present review will highlight the importance of herbal medicines and its components for treating RA via induction of apoptosis in FLS, and provide some directions for the future development of these mentioned herbal medicines as anti-RA drugs in clinical.

6.
Environ Pollut ; : 113627, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31796321

RESUMO

Lead (Pb) is one of the predominant heavy metals in e-waste recycling arears and recognized as a notorious environmental neurotoxic substance. However, whether Pb at environmentally relevant concentrations could cause neurobehavioral alteration and even what kind of signaling pathway Pb exposure would disrupt in zebrafish were not fully uncovered. In the present study, 6 h postfertilization (hpf) zebrafish embryos were exposed to Pb at the concentrations of 0, 5, 10, and 20 µg/L until 144 hpf. Then the neurobehavioral indicators including locomotor, turnings and social behaviors, and the expressions of selected genes concerning brain-derived neurotrophic factor (BDNF) signaling were investigated. The results showed that significant changes were obtained under 20 µg/L Pb exposure. The hypoactivity of zebrafish larvae in locomotor and turning behaviors was induced during the dark period, while hyperactivity was observed in a two-fish social assay during the light period. The significantly downregulation of genes encoding BDNF, its receptor TrkB, and N-methyl-D-aspartate glutamate receptor (NMDAR) suggested the involvement of NMDAR-dependent BDNF signaling pathway. Overall, our study demonstrated that developmental exposure to Pb at environmentally relevant concentrations caused obvious neurobehavioral impairment of zebrafish larvae by disrupting the NMDAR-dependent BDNF signaling, which could exert profound ecological consequences in the real environment.

7.
Sci Rep ; 9(1): 18314, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31797949

RESUMO

Circular RNAs (circRNAs) are involved in many physiological functions. Whether circulating circRNAs serve as markers for coronary artery disease (CAD) is unknown. Seven CAD-related microarray datasets were downloaded from the Gene Expression Omnibus (GEO) database and were analyzed using clustering and functional enrichment to identify hub mRNAs and miRNAs. StarBase V3.0 and circinteractome databases were used to predict interactions between circRNAs and miRNAs whereas miRwalk and DIANA TOOLS were used to predict interactions between miRNAs and mRNAs. Altogether, this helped establish a circRNA-miRNA-mRNA triple network for diagnosis of CAD. Five non-coding RNAs (ncRNAs) were identified in our study population with the use of quantitative real-time PCR (RT-PCR). The prognostic values of circYOD1, hsa-miR-21-3p and hsa-miR-296-3p were evaluated using a receiver operating characteristic (ROC) curve. A CAD circRNA-miRNA-mRNA network was established from our analyses containing one circRNA, four miRNAs and thirteen mRNAs. After performing RT-PCR validation between CAD and non-CAD samples, only three ncRNAs of five ncRNAs showed significance for further analysis. The area under ROC curve (AUC) of circ-YOD1 was 0.824, the AUC of hsa-miR-21-3p was 0.731 and hsa-miR-296-3p was 0.776. The pairwise comparison results showed that circ-YOD1 had statistical significance (PYOD1-21 < 0.01 and PYOD1-296 < 0.05). The results of functional enrichment analysis of interacting genes and microRNAs showed that the shared circ-YOD1 may act as a new biomarker for CAD. Our investigation of the triple regulatory networks of circRNA-miRNA-mRNA in CAD revealed circ-YOD1 as a potential biomarker for CAD.

8.
Org Biomol Chem ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31799547

RESUMO

With easily available monosaccharides and steviol as starting materials, the first total synthesis of rebaudioside R with a xylosyl core in the C13-OH linked sugar chain was accomplished via two distinct approaches. The first approach features the stepwise installation of branch-sugar residues via an order of C2-OH first and then C3-OH of the xylosyl core, laying a firm foundation for the synthesis of analogues with different branch sugars, while the second route features the introduction of the C13 trisaccharide sugar chain via a convergent strategy, securing the overall synthetic efficiency. Through the synthetic study, the effect of protecting groups (PGs) at the vicinal hydroxy group on the reactivity of OH acceptors was illustrated.

9.
Brain Lang ; 201: 104714, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31790907

RESUMO

The framework of embodied cognition has challenged the modular view of a language-cognition divide by suggesting that meaning-retrieval critically involves the sensory-motor system. Despite extensive research into the neural mechanisms underlying language-motor coupling, it remains unclear how the motor system might be differentially engaged by different levels of linguistic abstraction and language proficiency. To address this issue, we used fMRI to quantify neural activations in brain regions underlying motor and language processing in Chinese-English speakers' processing of literal, metaphorical, and abstract language in their L1 and L2. Results overall revealed a response in motor ROIs gradually attenuating in intensity from literal to abstract via metaphorical language in both L1 and L2. Furthermore, contrast analyses between L1 and L2 showed overall greater activations of motor ROIs in the L2. We conclude that motor involvement in language processing is graded rather than all-or-none and that the motor system has a dual-functional role.

10.
PLoS One ; 14(12): e0225115, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800586

RESUMO

WRI1 is a plant-specific transcription factor that enhances the accumulation of oils through the upregulation of the expression of genes involved in glycolysis and fatty acid biosynthesis. In this study, the EgWRI1 promoter from oil palm was isolated and characterized in transgenic Arabidopsis. The sequence analysis results revealed that various putative plant regulatory elements are present in the EgWRI1 promoter region. The EgWRI1 promoter and beta-glucuronidase (GUS) reporter gene were transcriptionally fused and transformed into Arabidopsis thaliana. Histochemical analysis revealed that GUS staining was very strong in whole seedlings, especially the stems, leaves, and siliques. Moreover, GUS staining was strong in the silique coats but weak in the seeds. Furthermore, to detect whether EgWRI1 was induced by environmental stress, we detected the expression efficiency of the EgWRI1 promoter in transgenic Arabidopsis treated with low temperature, darkness, and exogenous ethylene. The results showed that the activity of the EgWRI1 promoter was induced by darkness but suppressed significantly when exposed to exogenous ethylene. When treated with low temperature, the activity of the EgWRI1 promoter was first reduced after 24 hours but recovered after 48 hours. Taken together, these results reveal the features of the EgWRI1 promoter from oil palm, which will be helpful for improving oil accumulation in oil palm via reasonable cultivation methods.

11.
Acta Crystallogr C Struct Chem ; 75(Pt 12): 1593-1604, 2019 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-31802748

RESUMO

A novel hydrolytic stable CoII-organic framework, namely poly[[bis(2-amino-4-sulfonatobenzoato-κO1)tetraaquatris{µ-1,4-bis[(imidazol-1-yl)methyl]benzene-κ2N3:N3'}dicobalt(II)] tetrahydrate], {[Co(C7H5NO5S)(C14H14N4)1.5(H2O)2]·2H2O}n, (1), based on multifunctional 2-amino-5-sulfobenzoic acid (H2asba) and the auxiliary flexible ligand 1,4-bis[(imidazol-1-yl)methyl]benzene (bix), was prepared using the solution evaporation method. The purity of (1) was confirmed by elemental analysis and powder X-ray diffraction (PXRD) analysis. Complex (1) shows a novel 1D→2D interpenetrating network, which is further extended into a 3D supramolecular framework with channels occupied by the lattice water molecules. The 2-amino-4-sulfonatobenzoate (asba2-) ligand adopts a monodentate coordination mode. The bix ligands exhibit gauche-gauche (GG) and trans-trans (TT) conformations. A detailed analysis of the solid-state diffuse-reflectance UV-Vis spectrum reveals that an indirect band gap exists in the complex. The band structure, the total density of states (TDOS) and the partial density of states (PDOS) were calculated using the CASTEP program. The calculated band gap (Eg) matches well with the experimental one. The complex exhibits a reversible dehydration-rehydration behaviour. Interestingly, gas sorption experiments demonstrate that the new fully anhydrous compound obtained by activating complex (1) at 400 K shows selective adsorption of CO2 over N2. Complex (1) retains excellent framework stability in a variety of solvents and manifests distinct solvent-dependent fluorescence properties. Moreover, the complex shows multiresponsive fluorescence sensing for some nitroaromatics in aqueous medium.

12.
Medicine (Baltimore) ; 98(49): e18272, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31804366

RESUMO

RATIONALE: Hallermann-Streiff syndrome (HSS) is a rare congenital disorder characterized by craniofacial malformations, sparse hair, degenerative skin changes, eye abnormalities, dental defects, and proportionate short stature. PATIENT CONCERNS: A 24-year-old Chinese male patient presented to the ophthalmologist because of his sore eye and blurred vision. DIAGNOSES: The final diagnosis of presented case is HSS having the main features of the syndrome, however, associated with uncommon ocular features, ultrasound biomicroscopy (UBM) and optical coherence tomography (OCT)changes, including aphakia, glaucoma, long eye axes, cilliary abnormalities, and chorioretinal atrophy. INTERVENTIONS: Antiglaucomatous medical therapy failed to reduce the pressure in the right eye and a cyclocryotherapy was carried out. The antiglaucoma eye drops was continued in the left eye. OUTCOMES: The intraocular pressure has been reduced to the normal range, but the vision has not improved. LESSONS: In the diagnosis of HSS, we should not ignore the extraordinary information especially uncommon ophthalmic features, UBM and OCT changes. We highlight the necessity of a multidisciplinary approach for accurate diagnosis and appropriate management.

13.
Bioresour Technol ; 296: 122336, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31704603

RESUMO

Bauhinia variegate flower (BVF) was supposed to improve silage fermentation due to its abundant active components. Thus, corn stalk and stylo were ensiled with addition of 0, 5% or 10% BVF, and then ensiling characteristics, protein fraction and bacterial community were analyzed after 60-day fermentation. The contents of butyric acid (2.9 vs not detected, 13.2 vs 3.0 g/kg DM in corn stalk and stylo silage, respectively), ammonia-N (100.2 vs 83.2, 110.8 vs 61.9 g/kg total N) and free amino acid (35.6 vs 16.5, 35.0 vs 16.4 g/kg total N) were decreased in 10% BVF treated silages. The bacterial diversity was increased, where the relative abundance of Enterobacter or Clostridium decreased and that of lactic acid producing bacteria such as Lactobacillus, Weissella or Enterococcus increased. It is suggested that BVF could be used to improve fermentation quality and nutrient preservation of high-moisture corn stalk and stylo silage.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31714218

RESUMO

Surgical resection is the main clinical method for the treatment of bone tumors. A critical procedure for bone tumor resection is to plan a set of cut planes that enable resecting the bone tumor with a safe margin while preserving the maximum amount of healthy bone. Currently, the surgeons rely on manual methods to plan the cut planes, which highly depend on the surgeons' experiences and have been demonstrated to be error-prone, and in turn, increase the recurrence rate or resect much healthy bone. This study targets on improving the precision of cut plane planning for the image guided pelvis tumor resection surgeries. A semi-automatic approach to cut plane planning was proposed via a coarse-to-fine strategy. It can efficiently identify a dangerous region in the 3D space, which contains the bone tumor and its surrounding normal tissue with a safe margin. By projecting the dangerous region into an appropriate 2D space, a segmented boundary-constrained linear regression method was leveraged to plan a set of 3D cut planes that ensure the minimum area of the resected specimen in the 2D space while having the dangerous region cleared. Further, a coarse-to-fine 3D cut plane planning method was developed by incorporating a 3D cut plane refinement scheme with our 2D planning method. Extensive experiments, on the surgical data from nine previous pelvis tumor resection surgeries, demonstrated that our proposed approach substantially improved the localization precision of cut planes (p<0.001) and decreased the amount of resected specimen (p<0.05), as compared to the manual method.

15.
Medicine (Baltimore) ; 98(44): e17437, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689748

RESUMO

This study aimed to investigate the effect of a patient education and rehabilitation program (PERP) on anxiety, depression, and quality of life in muscle invasive bladder cancer (MIBC) patients underwent adjuvant chemotherapy.One hundred and thirty MIBC patients about to receive adjuvant chemotherapy with 4-cycle gemcitabine and cisplatin (GC) regimen (16 weeks) were consecutively enrolled and randomly allocated into PERP group and control group as 1:1 ratio. Hospital Anxiety and Depression Scale (HADS) anxiety and depression scores and Quality of Life Questionnaire (QLQ-C30) scores were assessed before treatment (W0) and after treatment (W16).After 16-week treatment, PERP group exhibited decreased HADS anxiety score (P = .036), ΔHADS anxiety score (W16-W0) (P < .001) and percentage of anxiety patients (P = .019) compared to control group. And PERP group presented with numerically reduced HADS depression score but without statistical significance (P = .076) compared to control group, while lower ΔHADS depression score (W16-W0) (P = .014) and percentage of depression patients (P = .015) compared to control group. As to quality of life, QLQ-C30 global health status score (P = .032), Δglobal health status score (W16-W0) (P = .003) and Δfunctional score (W16-W0) (P = .005) were higher in PERP group compared to control group. However, no difference of QLQ-C30 functional score (P = .103), QLQ-C30 symptom score (P = .808) or Δsymptom score (W16-W0) (P = .680) was observed between two groups.PERP relieves anxiety, depression and improves quality of life in MIBC patients underwent adjuvant chemotherapy.

17.
Toxicol Sci ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31693140

RESUMO

The effect of variations in the expression of cytochrome P450 reductase (CPR or POR) is determined in mice with decreased POR expression to identify potential vulnerabilities in people with low POR expression. There is an age-dependent appearance of increasing vacuolization in the proximal tubules of the renal cortex in 4-9-month-old male (but not female) Cpr-low mice. These mice have low POR expression in all cells of the body and upregulation of lysosomal-associated membrane protein 1 expression in the renal cortex. Vacuolization is also seen in extrahepatic Cpr-low and extrarenal Cpr-low male mice, but not in mice with tissue-specific Por deletion in liver, intestinal epithelium, or kidney. The occurrence of vacuolization is accompanied by increases in serum blood-urea-nitrogen levels. Male Cpr-low mice are hypersensitive to cisplatin and gentamicin induced renal toxicity at 3-months of age, before proximal tubular vacuoles are detectable. At doses that do not cause renal toxicity in wild-type mice, both drugs cause substantial increases in serum blood-urea-nitrogen levels and proximal tubular vacuolization in male but not female Cpr-low mice. The hypersensitivity to drug-induced renal toxicity is accompanied by increases in circulating drug levels. These novel findings demonstrate deficiency of renal function in mice with globally reduced POR expression and suggest that low POR expression may be a risk factor for drug-induced nephrotoxicity in humans.

18.
Ultrason Sonochem ; 61: 104813, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31670251

RESUMO

In this paper, in order to investigate the effects of ultrasound irradiation on the higher alcohols of wines, the parameters including ultrasound time, temperature and power were optimized by the response surface methodology, and the model wine solution was employed to explore the mechanism of ultrasonically decreasing the higher alcohols. The results indicate that the maximum decreasing of higher alcohols could be obtained under the ultrasound conditions of 30 min, 30 °C and 150 W, and the final content was 306.75 mg/L with the reduction rate of 40.44%, suggesting a modification of wine quality due to the negative effects of excessive contents on wine. Regarding the results of model wine, it indicates that the decrease could be definitely affected by factors, such as tartaric acid and ions in wine, which might be attributed to the free radicals generated from ultrasound cavitation and its subsequent reactions. In summary, all the results may help to understand the effects of ultrasound irradiation on improving the sensory properties of wine by decreasing the higher alcohols.

19.
Age Ageing ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31746329

RESUMO

BACKGROUND: although handgrip strength (HGS) is considered an excellent predictor of morbidity and mortality, there are few reference data available, especially across life from young to old populations. OBJECTIVE: to investigate the reference values and determinants for HGS in a large Chinese adult population. DESIGN: this cross-sectional study used data from the Tianjin Chronic Low-Grade Systemic Inflammation and Health Cohort Study ranging from 2013 to 2017. SETTING AND SUBJECTS: in total, 37,707 Chinese adults aged 18-93 years with measures of HGS were included. METHODS: HGS was measured using a handheld digital dynamometer. The percentile distribution of HGS, as well as mean (standard deviation) values of HGS, was calculated according to sex and age categories. The Pearson's or Spearman's correlation coefficient and multiple linear regression were also used. RESULTS: the peak mean value of HGS was 45.2 ± 6.88 kg in men and 26.8 ± 4.65 kg in women. Different reference values for each sex and age category are present. The prevalence of low HGS was 7.69% in men and 6.46% in women. Multiple regression analysis showed that almost 72.5% of HGS variability can be explained by sex, age, height and weight. CONCLUSIONS: the HGS reached peak at 40 years old, maintained or slightly lowered between ages 40 and50 and then start an accelerated decline after 50 years old. Reference values of HGS should be stratified by sex and age group. HGS variability can be largely explained by sex, age, height and weight.

20.
Bioresour Technol ; : 122390, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31740244

RESUMO

Whether tannins reducing proteolysis is related to protease or the antioxidant capacity could be preserved during Moringa oleifera leaves (MOL) ensiling are unclear. In the present study, MOL ensiled with 1% and 2% gallic acid (GA) or tannic acid (TA) were analyzed for fermentation parameters, protein fractions, protease activities and antioxidant capacity on day 3, 7, 14 and 30 of ensiling. The results showed that GA and TA decreased dry matter loss (11.13% vs 8.35% and 3.11%, in the level of 2%) and proteolysis (nonprotein-N: 56.31% vs 32.64% and 26.04% TN). Meanwhile, GA and TA increased pH, yeasts number, ammonia-N content and aminopeptidase activity. The antioxidant capacity of MOL silage gradually increased during ensiling, and was dramatically enhanced by the addition of GA and TA. In conclusion, this study suggests that GA and TA could help to improve the preservation of protein and antioxidant capacity during MOL ensiling.

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