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1.
J Immunother ; 43(1): 16-28, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31574023

RESUMO

Treatment with chimeric antigen receptor-modified T cell (CAR-T) has demonstrated promising therapeutic efficacy in hematologic malignancies. However, the therapeutic efficacy is still very limited for solid tumors. An immunosuppressive microenvironment is one of the main reasons for the limited efficacy. Some chemotherapeutic agents exhibit immune microenvironment modulation. Therefore, combination with chemotherapeutic agents may be one of the promising strategies to enhance the therapeutic efficacy of CAR-T against solid tumors. Sunitinib modulates the antitumor immune response by improving T-cell infiltration and function while reducing immunosuppressive factors. The authors constructed a second-generation CAR targeting human renal cell carcinoma (RCC)-specific antigen carbonic anhydrase IX (CAIX) with the costimulatory domain of 4-1BB. The results of cytokine releasing and cell killing assays showed that the CAIX-CAR-T cells have specific effector functions against CAIX renal cancer cells in vitro. Combination therapy with CAIX-CAR-T and sunitinib showed synergistic efficacy against a mouse lung metastasis model of human RCC. CAIX-CAR-T cells in the mice of the combination therapy group showed stronger proliferation and tumor infiltration than that in the mice of the CAIX-CAR-T monotherapy group. The possible mechanisms of the synergistic efficacy are: (1) sunitinib caused upregulation of CAIX in tumor cells; (2) sunitinib decreased frequency of myeloid-derived suppressor cells in the tumor microenvironment. Our study supplied an innovative immunotherapeutic approach whereby combining CAIX-CAR-T with sunitinib induces a potent antitumor response in an experimental model of metastatic RCC. The combination strategy should be considered as a potential approach to augment adoptive CAR-T cell immunotherapy.

2.
Bioresour Technol ; 295: 122255, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31639626

RESUMO

To investigate the feasibility of vegetal gallic acid (GA) improving silage quality, fermentation parameter, nitrogen distribution and bacterial community of mulberry leaves and stylo ensiled with 1% and 2% GA were analyzed after 60-d fermentation. The results showed that GA addition decreased dry matter loss (6.08% vs 5.35%, 17.79% vs 11.56% in mulberry leaves and style silage, respectively), pH (6.51 vs 5.98, 5.55 vs 4.57), butyric acid (0.41% and 0.83% DM, undetected in GA groups) and ammonia-N (0.71% vs 0.19%, 1.46% vs 0.29% TN) contents and increased lactic acid (2.27% vs 6.68%, 0.91% vs 1.91% DM) and acetic acid (1.68% vs 3.20%, 0.97% vs 2.02% DM) contents. Meanwhile, the relative abundance of Clostridium or Enterobacter was decreased, and that of lactate-producing bacteria was increased in mulberry leaves and stylo silage. In conclusion, GA could be used as a green additive to improve fermentation quality and protein preservation during ensiling.


Assuntos
Morus , Silagem , Bactérias , Fermentação , Ácido Gálico , Nitrogênio , Folhas de Planta
3.
Bioresour Technol ; 295: 122268, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31675519

RESUMO

To find a comprehensive way to enhance the utilizability of steam-exploded hippophae, calcium oxide (CaO) preimpregnation, cellulase-added storage and saccharification with addition of Tween 20 were investigated in this study. Both CaO preimpregnation and cellulase addition promoted the ensiling fermentation of anaerobically stored steam-exploded hippophae indicated by lower cellulose proportion and higher organic acids content, but led to the decrease of saccharification yield by 11.83% and 46.77-51.22%, respectively. When taking into account of organic acids being utilizable energy source, storing with addition of cellulase enhanced the utilizability of the materials in whole. Moreover, the addition of Tween 20 enhanced saccharification yield of the steam-exploded hippophae by 26.69-45.25%. Additionally, FTIR and XRD spectra clearly illustrated the structural alteration during storage. It is concluded that storing with addition of cellulase and hydrolyzing with addition of Tween 20 can enhance the utilizability of steam-exploded hippophae.


Assuntos
Celulase , Hippophae , Compostos de Cálcio , Hidrólise , Óxidos , Polissorbatos , Vapor
4.
Biomolecules ; 9(12)2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31795133

RESUMO

Rheumatoid arthritis (RA) is a known chronic autoimmune disease can cause joint deformity and even loss of joint function. Fibroblast-like synoviocytes (FLS), one of the main cell types in synovial tissues of RA patients, are key effector cells in the development of RA and are considered as promising therapeutic targets for treating RA. Herbal medicines are precious resources for finding novel agents for treating various diseases including RA. It is reported that induction of apoptosis in FLS is an important mechanism for the herbal medicines to treat RA. Consequently, this paper reviewed the current available references on pro-apoptotic effects of herbal medicines on FLS and summarized the related possible signal pathways. Taken together, the main related signal pathways are concluded as death receptors mediated apoptotic pathway, mitochondrial dependent apoptotic pathway, NF-κB mediated apoptotic pathways, mitogen-activated protein kinase (MAPK) mediated apoptotic pathway, endoplasmic reticulum stress (ERS) mediated apoptotic pathway, PI3K-Akt mediated apoptotic pathway, and other reported pathways such as janus kinase/signal transducers and activators of transcription (JAK-STAT) signal pathway. Understanding the apoptosis induction pathways in FLS of these herbal medicines will not only help clear molecular mechanisms of herbal medicines for treating RA but also be beneficial for finding novel candidate therapeutic drugs from natural herbal medicines. Thus, we expect the present review will highlight the importance of herbal medicines and its components for treating RA via induction of apoptosis in FLS, and provide some directions for the future development of these mentioned herbal medicines as anti-RA drugs in clinical.

5.
Environ Pollut ; : 113627, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31796321

RESUMO

Lead (Pb) is one of the predominant heavy metals in e-waste recycling arears and recognized as a notorious environmental neurotoxic substance. However, whether Pb at environmentally relevant concentrations could cause neurobehavioral alteration and even what kind of signaling pathway Pb exposure would disrupt in zebrafish were not fully uncovered. In the present study, 6 h postfertilization (hpf) zebrafish embryos were exposed to Pb at the concentrations of 0, 5, 10, and 20 µg/L until 144 hpf. Then the neurobehavioral indicators including locomotor, turnings and social behaviors, and the expressions of selected genes concerning brain-derived neurotrophic factor (BDNF) signaling were investigated. The results showed that significant changes were obtained under 20 µg/L Pb exposure. The hypoactivity of zebrafish larvae in locomotor and turning behaviors was induced during the dark period, while hyperactivity was observed in a two-fish social assay during the light period. The significantly downregulation of genes encoding BDNF, its receptor TrkB, and N-methyl-D-aspartate glutamate receptor (NMDAR) suggested the involvement of NMDAR-dependent BDNF signaling pathway. Overall, our study demonstrated that developmental exposure to Pb at environmentally relevant concentrations caused obvious neurobehavioral impairment of zebrafish larvae by disrupting the NMDAR-dependent BDNF signaling, which could exert profound ecological consequences in the real environment.

6.
Brain Lang ; 201: 104714, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31790907

RESUMO

The framework of embodied cognition has challenged the modular view of a language-cognition divide by suggesting that meaning-retrieval critically involves the sensory-motor system. Despite extensive research into the neural mechanisms underlying language-motor coupling, it remains unclear how the motor system might be differentially engaged by different levels of linguistic abstraction and language proficiency. To address this issue, we used fMRI to quantify neural activations in brain regions underlying motor and language processing in Chinese-English speakers' processing of literal, metaphorical, and abstract language in their L1 and L2. Results overall revealed a response in motor ROIs gradually attenuating in intensity from literal to abstract via metaphorical language in both L1 and L2. Furthermore, contrast analyses between L1 and L2 showed overall greater activations of motor ROIs in the L2. We conclude that motor involvement in language processing is graded rather than all-or-none and that the motor system has a dual-functional role.

7.
PLoS One ; 14(12): e0225115, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800586

RESUMO

WRI1 is a plant-specific transcription factor that enhances the accumulation of oils through the upregulation of the expression of genes involved in glycolysis and fatty acid biosynthesis. In this study, the EgWRI1 promoter from oil palm was isolated and characterized in transgenic Arabidopsis. The sequence analysis results revealed that various putative plant regulatory elements are present in the EgWRI1 promoter region. The EgWRI1 promoter and beta-glucuronidase (GUS) reporter gene were transcriptionally fused and transformed into Arabidopsis thaliana. Histochemical analysis revealed that GUS staining was very strong in whole seedlings, especially the stems, leaves, and siliques. Moreover, GUS staining was strong in the silique coats but weak in the seeds. Furthermore, to detect whether EgWRI1 was induced by environmental stress, we detected the expression efficiency of the EgWRI1 promoter in transgenic Arabidopsis treated with low temperature, darkness, and exogenous ethylene. The results showed that the activity of the EgWRI1 promoter was induced by darkness but suppressed significantly when exposed to exogenous ethylene. When treated with low temperature, the activity of the EgWRI1 promoter was first reduced after 24 hours but recovered after 48 hours. Taken together, these results reveal the features of the EgWRI1 promoter from oil palm, which will be helpful for improving oil accumulation in oil palm via reasonable cultivation methods.

8.
Sci Rep ; 9(1): 18314, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31797949

RESUMO

Circular RNAs (circRNAs) are involved in many physiological functions. Whether circulating circRNAs serve as markers for coronary artery disease (CAD) is unknown. Seven CAD-related microarray datasets were downloaded from the Gene Expression Omnibus (GEO) database and were analyzed using clustering and functional enrichment to identify hub mRNAs and miRNAs. StarBase V3.0 and circinteractome databases were used to predict interactions between circRNAs and miRNAs whereas miRwalk and DIANA TOOLS were used to predict interactions between miRNAs and mRNAs. Altogether, this helped establish a circRNA-miRNA-mRNA triple network for diagnosis of CAD. Five non-coding RNAs (ncRNAs) were identified in our study population with the use of quantitative real-time PCR (RT-PCR). The prognostic values of circYOD1, hsa-miR-21-3p and hsa-miR-296-3p were evaluated using a receiver operating characteristic (ROC) curve. A CAD circRNA-miRNA-mRNA network was established from our analyses containing one circRNA, four miRNAs and thirteen mRNAs. After performing RT-PCR validation between CAD and non-CAD samples, only three ncRNAs of five ncRNAs showed significance for further analysis. The area under ROC curve (AUC) of circ-YOD1 was 0.824, the AUC of hsa-miR-21-3p was 0.731 and hsa-miR-296-3p was 0.776. The pairwise comparison results showed that circ-YOD1 had statistical significance (PYOD1-21 < 0.01 and PYOD1-296 < 0.05). The results of functional enrichment analysis of interacting genes and microRNAs showed that the shared circ-YOD1 may act as a new biomarker for CAD. Our investigation of the triple regulatory networks of circRNA-miRNA-mRNA in CAD revealed circ-YOD1 as a potential biomarker for CAD.

9.
Org Biomol Chem ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31799547

RESUMO

With easily available monosaccharides and steviol as starting materials, the first total synthesis of rebaudioside R with a xylosyl core in the C13-OH linked sugar chain was accomplished via two distinct approaches. The first approach features the stepwise installation of branch-sugar residues via an order of C2-OH first and then C3-OH of the xylosyl core, laying a firm foundation for the synthesis of analogues with different branch sugars, while the second route features the introduction of the C13 trisaccharide sugar chain via a convergent strategy, securing the overall synthetic efficiency. Through the synthetic study, the effect of protecting groups (PGs) at the vicinal hydroxy group on the reactivity of OH acceptors was illustrated.

10.
Mikrochim Acta ; 186(12): 860, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31786663

RESUMO

An aptasensor is described for the electrochemical determination of bisphenol A (BPA). Gold-coated multiwalled carbon nanotubes (Au/MWCNTs) and a single-stranded DNA-dye complex are used as a double signal-amplification system. The BPA-binding aptamer was assembled on a disposable electrode modified with Au/MWCNTs. Methylene blue (MB) was then intercalated into the immobilized aptamer with an approximately molecular ratio of 4 to form a complex. Upon interaction with BPA, the immobilized aptamer underwent a conformational change. This causes the intercalated MB to be released from the complex into solution. As a result, the electrochemical signal of the intercalated MB, typically measured using square wave voltammetry at a potential of -0.20 V (vs. Ag/AgCl (saturated KCl)) decreases. The fabrication of the aptasensor was characterized by the scanning electron microscopy, atomic force microscopy, and electrochemical techniques. Under optimal experimental conditions, the current drops linearly with the logarithm of BPA concentrations over the range from 10 fM to 1 nM, and the limit of detection is 8 fM. The assay was applied to the determination of BPA in plastic drinking bottles, tap water, and milk. Graphical AbstractSchematic illustration of fabricating the aptasensor for bisphenol A (BPA) based on double signal amplification via gold-coated multiwalled carbon nanotubes (Au/MWCNT) and an aptamer-dye complex. PET: poly(ethylene terephthalate).

11.
Acta Crystallogr C Struct Chem ; 75(Pt 12): 1593-1604, 2019 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-31802748

RESUMO

A novel hydrolytic stable CoII-organic framework, namely poly[[bis(2-amino-4-sulfonatobenzoato-κO1)tetraaquatris{µ-1,4-bis[(imidazol-1-yl)methyl]benzene-κ2N3:N3'}dicobalt(II)] tetrahydrate], {[Co(C7H5NO5S)(C14H14N4)1.5(H2O)2]·2H2O}n, (1), based on multifunctional 2-amino-5-sulfobenzoic acid (H2asba) and the auxiliary flexible ligand 1,4-bis[(imidazol-1-yl)methyl]benzene (bix), was prepared using the solution evaporation method. The purity of (1) was confirmed by elemental analysis and powder X-ray diffraction (PXRD) analysis. Complex (1) shows a novel 1D→2D interpenetrating network, which is further extended into a 3D supramolecular framework with channels occupied by the lattice water molecules. The 2-amino-4-sulfonatobenzoate (asba2-) ligand adopts a monodentate coordination mode. The bix ligands exhibit gauche-gauche (GG) and trans-trans (TT) conformations. A detailed analysis of the solid-state diffuse-reflectance UV-Vis spectrum reveals that an indirect band gap exists in the complex. The band structure, the total density of states (TDOS) and the partial density of states (PDOS) were calculated using the CASTEP program. The calculated band gap (Eg) matches well with the experimental one. The complex exhibits a reversible dehydration-rehydration behaviour. Interestingly, gas sorption experiments demonstrate that the new fully anhydrous compound obtained by activating complex (1) at 400 K shows selective adsorption of CO2 over N2. Complex (1) retains excellent framework stability in a variety of solvents and manifests distinct solvent-dependent fluorescence properties. Moreover, the complex shows multiresponsive fluorescence sensing for some nitroaromatics in aqueous medium.

12.
Medicine (Baltimore) ; 98(49): e18272, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31804366

RESUMO

RATIONALE: Hallermann-Streiff syndrome (HSS) is a rare congenital disorder characterized by craniofacial malformations, sparse hair, degenerative skin changes, eye abnormalities, dental defects, and proportionate short stature. PATIENT CONCERNS: A 24-year-old Chinese male patient presented to the ophthalmologist because of his sore eye and blurred vision. DIAGNOSES: The final diagnosis of presented case is HSS having the main features of the syndrome, however, associated with uncommon ocular features, ultrasound biomicroscopy (UBM) and optical coherence tomography (OCT)changes, including aphakia, glaucoma, long eye axes, cilliary abnormalities, and chorioretinal atrophy. INTERVENTIONS: Antiglaucomatous medical therapy failed to reduce the pressure in the right eye and a cyclocryotherapy was carried out. The antiglaucoma eye drops was continued in the left eye. OUTCOMES: The intraocular pressure has been reduced to the normal range, but the vision has not improved. LESSONS: In the diagnosis of HSS, we should not ignore the extraordinary information especially uncommon ophthalmic features, UBM and OCT changes. We highlight the necessity of a multidisciplinary approach for accurate diagnosis and appropriate management.

13.
Opt Express ; 27(22): 32835-32845, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31684488

RESUMO

Absorbers have potential applications in the stealth field. However, limited bandwidth and low absorption rate persist in existing methods. Moreover, absorbers working in the low frequency range (1-4 GHz) with small size are much more difficult to realize. In this paper, we propose a novel absorption structure, which combines indium tin oxide film and metal resonator. The former realizes impedance matching with free space in a broad bandwidth at moderate frequency range while the latter shows the resonant property at low frequency. Based on this absorption structure, we design the zigzag-shaped structure to realize high-efficiency and ultra-broadband absorption. To demonstrate the feasibility of our method, we fabricate a sample and perform measurements. The measurement results show that our sample can achieve ultra-broadband absorption with high-efficiency of over 90% from 1 GHz to 18 GHz, which is in good agreement with simulation results. Our findings provide a valuable technique for broadband device design, which could bring about a wide range of applications in cloaking technology.

14.
Int J Biol Macromol ; 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31715239

RESUMO

Chuanminshen violaceum has been used as an important traditional Chinese medicine and a popular tonic food in China. Polysaccharides are considered the major bioactive components in C. violaceum. In this study, in order well understand the chemical structures and bioactivities of non-starch polysaccharides in C. violaceum (CVPs), the physicochemical structures, antioxidant activities, and immunomodulatory activities of CVPs in C. violaceum collected from different regions of China were investigated and compared. Results showed that the constituent monosaccharides and Fourier transform infrared spectra of CVPs in C. violaceum collected from different regions were similar. However, their molar ratios of constituent monosaccharides, molecular weights, and contents of uronic acids were different. Furthermore, CVPs exerted remarkable antioxidant activities (ABTS and nitric oxide radical scavenging capacities) and immunomodulatory activities (promoted production of nitric oxide, IL-6, and TNF-α from RAW 264.7 macrophages in vitro). Meanwhile, the antioxidant and immunomodulatory activities of CVPs extracted from C. violaceum also varied by cultivated regions. Moreover, results indicated that the antioxidant and immunomodulatory activities of CVPs were closely correlated to their α-1,4-d-galactosiduronic linkages. Results are helpful for better understanding of the structure-bioactivity relationships of CVPs, and beneficial for the improvement of their applications in pharmaceutical and functional food industries.

15.
Radiother Oncol ; 144: 30-36, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31710941

RESUMO

BACKGROUND AND PURPOSE: The aim of this study was to establish curves for the conversion of RBE-weighted doses for targets and organs at risk (OARs) from the microdosimetric kinetic model (MKM) calculation to that of the local effect model I (LEM) for carbon ion radiotherapy (CIRT) for prostate carcinoma (PCA). MATERIALS AND METHODS: This study was performed in the experimental treatment planning system (eTPS, V8A, Raystation, Sweden), which incorporates both MKM and LEM. CIRT plans from 10 PCA patients were collected. There were 5 steps to establish the curves: (1) design MKM plans in eTPS; (2) recalculate the physical doses from MKM to LEM and create a LEM plan in eTPS; (3) plot the RBE-weighted MKM to LEM conversion curves; (4) convert the MKM rectum constraint dose volume histogram (DVH) from NIRS to a LEM DVH; and (5) compare patients' rectum DVHs and follow-up with the converted constraint DVH. RESULTS: The conversion factors for MKM doses of 0.18 Gy (RBE) to 4.55 Gy (RBE) per fraction to LEM doses were 2.72-1.06. For fraction sizes of >1 Gy (RBE), the conversion factors matched Fossati's curve and for fraction sizes of <1.00 Gy (RBE) the values were on the extrapolated Fossati's curve. A LEM rectum constraint DVH was established. Ten patients' rectum DVHs were all lower than LEM constraint DVHs. No complications were reported clinically. CONCLUSION: For PCA receiving CIRT, the RBE-weighted doses using MKM for targets and OARs could be converted to LEM doses using conversion curves.

16.
Nanoscale ; 11(47): 22971-22979, 2019 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-31769772

RESUMO

As a coordination polymer built of [Mo3S13]2- clusters, amorphous nanoscale MoSx (a-MoSx) is an attractive electrocatalyst for the hydrogen evolution reaction (HER) due to its abundant active sites and scalable synthesis. However, clarifying the internal catalytic mechanism and achieving even higher HER performance with scalable size are still challenging. Herein, a new hybrid catalyst of a-MoSx flakes decorated with Ni3S2 nanocrystals (size < 10 nm) has been successfully synthesized on 10 × 20 cm2-sized Ni foam by a portable hydrothermal route. As the strong interaction of [Mo3S13]2- clusters with Ni3S2 is evidenced by comprehensive binding state and Raman characterization, the polymerization effect of [Mo3S13]2- itself and the perfect interfaces between [Mo3S13]2- clusters and Ni3S2 are also confirmed by density functional theory calculations. These two factors greatly lower the absorption energy of hydrogen nearly to zero, leading to much improved HER activity. Current densities of 100 and 600 mA cm-2 are achieved at overpotentials of 181 and 246 mV, respectively, which are so far the highest values approaching practical applications. The findings of this work provide a fundamental reference about the catalytic origin of a-MoSx based catalysts, and shed light on the practical applications of non-precious electrocatalysts for their compatibility with low cost batch production.

17.
J Nucl Med ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31757845

RESUMO

Purpose: To explore the value of 68Ga-PSMA-PET/CT for detection of phosphatase and tensin homolog (PTEN) - loss prostate cancer (PCa). Methods: We retrospectively enrolled 75 patients who underwent multiparametric MRI (mpMRI) and 68Ga-PSMA PET/CT before radical prostatectomy. Lesions were outlined on pathological images and regions of interest were drawn on matched mpMRI and PET/CT images. Imaging parameters including average apparent diffusion coefficient (ADCmean) and maximum standardized uptake value (SUVmax) were derived. Immunohistochemical staining was carried out to evaluate the PTEN status. The diagnostic performance of imaging parameters was analyzed by receiver operating characteristics (ROC) analysis. A univariate logistic regression analyses were used to evaluate the association between clinical and imaging variables and PTEN status. Results: Totally, 103 lesions from 54 patients were analyzed. Of these lesions, 34 of 103 (33.0%) showed PTEN-loss status. Our study showed a strong association between SUVmax and PTEN-loss tumors both in the per-patient analysis (P < 0.01) and per-lesion analysis (P < 0.01), yielding the sensitivity and specificity of 0.80 and 0.77 in the per-patient analysis and 0.83 and 0.74 in the per-lesion analysis. Meanwhile, higher pathological PSMA expression was found in the PTEN-deficiency tumors. However, there was no significant difference between PTEN-loss tumors and PTEN-intact tumors using parameters including ADCmean (P > 0.05) and PI-RADS score (P > 0.05). Surprisingly, SUVmax was a significant predictor for detection of PTEN-loss tumors (odds ratio: 7.56, 95% confidence interval: 2.18-26.24, per-patient analysis; odds ratio: 13.66, 95% confidence interval: 4.32-43.24, per-lesion analysis). Conclusion: 68Ga-PSMA-PET/CT could effectively detect aggressive PTEN-loss tumors.

18.
Analyst ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31748765

RESUMO

Two types of lateral flow immunochromatographic test strips (LFITS) using gold nanoparticles and fluorescent CdTe quantum dots (QDs) as signal labels, respectively, were developed for Shiga toxin type II (STX2) assays. Under optimal conditions, the corresponding visual detection limits were 25 ng mL-1 and 5 ng mL-1, respectively. The test results of gold based LFITS can be recognized directly by the naked eye, whereas the visualized results of CdTe QDs based LFITS can be observed by the aid of a UV lamp. Both assays showed good specificity and stability. The inexpensive LFITS were promising for the rapid clinical detection of STX2.

19.
Clin Exp Hypertens ; : 1-8, 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31760826

RESUMO

Background: Honey has been shown to have multiple positive effects on human health. However, data on the associations of honey consumption with prehypertension are scarce. The aim of this study was to investigate whether honey consumption is associated with prehypertension in a Chinese population. Method: A cross-sectional study was conducted in 4,561 individuals aged ≥40 years. A validated 100-item semi-quantitative food frequency questionnaire was used to assess honey consumption. Blood pressure was measured at least twice by trained nurses. Multiple logistic regression models were used to explore the associations between honey consumption and prehypertension. Results: After adjustment for potential confounders, the odds ratios and 95% confidence intervals of prehypertension across increasing frequency of honey consumption were 1.00 (reference) for almost never, 1.17 (0.96, 1.41) for ≤6 times/week, and 1.25 (0.86, 1.84) for ≥7 times/week in men (P for trend = 0.09); 1.00 (reference) for almost never, 0.76 (0.62, 0.92) for ≤6 times/week, and 0.84 (0.63, 1.12) for ≥7 times/week in women (P for trend = 0.055), respectively. Conclusion: Our results showed that light-to-moderate intake of honey was associated with lower prevalence of prehypertension in women, but not men. Future studies are required to confirm these associations.

20.
Exp Neurol ; 323: 113084, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31697945

RESUMO

OBJECTIVE: To establish an animal model for posttraumatic stress disorder in burn-injured patients. METHODS: Thermal-injured mice with 15% total body surface area were subjected to a series of neurobehavioral tests at 1 and 3 months postburn. Brains were collected for analysis of key molecules expression, spleens for T cell function analysis, and blood for biochemistry and hormones detection. RESULTS: Comparison with sham mice, burn mice showed extremely high locomotion in homecage, open field, and forced swimming tests, indicating a hyper-arousal state. Burn mice exhibited improved spatial memory in Morris Water Maze test and heightened context fear memory in context fear conditioning, suggesting re-experiencing behavior. Although burn mice showed pronounced passive avoidance in the step-through test, their active avoidance capability in response to the conditional stimulus in the shuttle box test was relatively deteriorated. Likewise, the retention of cue-feared memory was impaired in fear conditioning test. The above negative alterations in mood were recapitulated in open-field test, in which the burn mice displayed an anxiety-like behavior with less time spent in the center. However, no sign of depression was found in the forced swimming and sucrose preference tests. The negative mood of burn mice was reinforced by a deficit in sociality and preference for social novelty in social interaction test. These neurobehavioral alterations were associated with an increased expression of brain-derived neurotrophic factor along with a remarkable microgliosis and a moderate astrocytosis in the brain of burn vs. sham mice. Moreover, a prominent Th2 switch and consequent increased nuclear NF-κB translocation were seen in the splenic T cells from burn relative to sham mice. CONCLUSIONS: We conclude that even mild burn injury could lead to long-lasting cognitive and effective alterations in mice. These findings shed light on the interactions among neuropsychology, neurobiology, and immunology throughout the recovery period of burn injury.

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