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1.
Nat Commun ; 12(1): 4654, 2021 08 02.
Artigo em Inglês | MEDLINE | ID: mdl-34341342

RESUMO

Ligand-oligonucleotide transduction provides the critical pathway to integrate non-nucleic acid molecules into nucleic acid circuits and nanomachines for a variety of strand-displacement related applications. Herein, a general platform is constructed to convert the signals of ligands into desired oligonucleotides through a precise kinetic control. In this design, the ligand-aptamer binding sequence with an engineered duplex stem is introduced between the toehold and displacement domains of the invading strand to regulate the strand-displacement reaction. Employing this platform, we achieve efficient transduction of both small molecules and proteins orthogonally, and more importantly, establish logical and cascading operations between different ligands for versatile transduction. Besides, this platform is capable of being directly coupled with the signal amplification systems to further enhance the transduction performance. This kinetically controlled platform presents unique features with designing simplicity and flexibility, expandable complexity and system compatibility, which may pave a broad road towards nucleic acid-based developments of sophisticated transduction networks.


Assuntos
Aptâmeros de Nucleotídeos/química , Técnicas de Amplificação de Ácido Nucleico/métodos , Conformação de Ácido Nucleico , Oligonucleotídeos/química , Aptâmeros de Nucleotídeos/genética , Aptâmeros de Nucleotídeos/metabolismo , Cinética , Ligantes , Modelos Genéticos , Modelos Moleculares , Oligonucleotídeos/genética , Oligonucleotídeos/metabolismo , Transdução de Sinais/genética , Espectrometria de Fluorescência/métodos
2.
Artigo em Inglês | MEDLINE | ID: mdl-24055753

RESUMO

High-speed counter-current chromatography (HSCCC) was successfully applied for the first time to isolate and purify four cis-trans isomers of coumaroylspermidine analogs from Safflower. HSCCC separation was achieved with a two-phase solvent system composed of chloroform-methanol-water (1:1:1, v/v/v) with the upper phase as the mobile phase. In a single run, a total of 1.3mg of N(1), N(5), N(10)-(E)-tri-p-coumaroylspermidine (EEE), 4.4mg of N(1)(E)-N(5)-(Z)-N(10)-(E)-tri-p-coumaroylspermidine (EZE), 7.2mg of N(1)(Z)-N(5)-(Z)-N(10)-(E)-tri-p-coumaroylspermidine (ZZE), and 11.5mg of N(1),N(5),N(10)-(Z)-tri-p-coumaroylspermidine (ZZZ) were obtained from 100mg of crude sample. High Performance Liquid Chromatography (HPLC) analysis showed that the purities of these four components are 95.5%, 98.1%, 97.5% and 96.2%, respectively. The chemical structures were identified by ESI-MS, (1)H NMR and (13)C NMR.


Assuntos
Carthamus tinctorius/química , Ácidos Cumáricos/isolamento & purificação , Distribuição Contracorrente/métodos , Espermidina/análogos & derivados , Espermidina/isolamento & purificação , Ácidos Cumáricos/análise , Ácidos Cumáricos/química , Isomerismo , Modelos Moleculares , Extratos Vegetais/química , Espermidina/análise , Espermidina/química
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