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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 282: 121679, 2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35917619

RESUMO

We theoretically investigate the possibility of laser cooling 87Sr35Cl molecule in accordance with vibrational and hyperfine spectroscopy. The potential energy curves and dipole moment of the X2Σ+1/2, A2Π1/2,3/2 and B2Σ+ states are calculated using ab initio method and the spectroscopic parameters are in good agreement with the experimental data. On account of the accurate potential energy curves and the transition dipole moment, the Franck - Condon factors and radiative lifetimes are predicted. Comparing the conditions of laser cooling candidate molecules, the A2Π 1/2 â†” X2Σ+1/2 transition is selected as the laser cooling cycle system. In order to obtain an approximately closed cooling cycle system, we employed matrix element algorithm to calculated the hyperfine spectroscopy and branching ratios of the 87Sr35Cl molecule. Furthermore, an electro-optical modulator (EOM) is designed including six hyperfine levels of the ground state X2Σ+1/2 (v = 0, N = 1).

2.
Mikrochim Acta ; 189(8): 308, 2022 08 02.
Artigo em Inglês | MEDLINE | ID: mdl-35916935

RESUMO

Chemiresistive ammonia (NH3) detection at room temperature is highly desired due to the unique merits of easy miniaturization, low cost, and minor energy consumption especially for portable and wearable electronics. In this regard, poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) has sparked considerable attention due to the benign room-temperature conductivity and environmental stability, but it is undesirably impeded by limited sensitivity and sluggish reaction kinetics. To overcome these, we incorporated cellulose nanofibers (CNF) into PEDOT:PSS via a facile blending. The constituent-optimized composite sensor displayed sensitive (sensitivity of ∼7.46%/ppm in the range of 0.2-3 ppm), selective, and stable NH3 sensing at 25 °C at 55% RH, with higher response and less baseline drift than pure PEDOT:PSS counterparts. Additionally, the response/recovery times (4.9 s/5.2 s toward 1 ppm NH3) ranked the best cases of conducting polymers based NH3 sensors. The humidity involved more than twofold response enhancement indicated a huge potential in exhaled breath monitoring. Furthermore, we observed an excellent flexible NH3-sensing performance with bending-tolerant features. This work provides an alternative strategy for trace NH3 sensing with low power consumption, superfast reaction, and high sensitivity.


Assuntos
Celulose , Nanofibras , Amônia , Compostos Bicíclicos Heterocíclicos com Pontes , Polímeros
3.
Sci Total Environ ; 847: 157442, 2022 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-35901874

RESUMO

The behavior and fate of PAHs are affected by multiple meteorological factors, but the main factors driving PAHs in tropical mariculture areas are still not clearly understood. This study continuously monitored PAHs in a few tropical land-based mariculture ponds, discussed their dynamic change trend, migration among the multiple media, and the relevant affected factors. Results indicated that PAHs were widely distributed in these environmental media, and the PAHs' concentration showed an obvious attenuation trend in the mariculture cycle. Wet deposition brought overwhelming majority atmospheric PAHs (92 % ± 5.7 %) to the aqueous system, and >72 % of these PAHs came from oil combustion-related sources and biomass combustion. Compared with the natural sea areas in the same region, mariculture ponds sediment could be changed from a sink at the early stage to a secondary release source of PAHs at the late stage of the rainy season, which intensifies the bioaccumulation of PAHs and the risk of edible carcinogenesis of aquatic products. Our research revealed that rainfall drove the occurrence and environmental behavior of PAHs in the tropical mariculture areas, while land-based mariculture ponds ecosystem affected the regional environmental fate of PAHs and weakened their transmission to the marine environment from land.

4.
Genomics ; 114(4): 110435, 2022 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-35878812

RESUMO

Systemic lupus erythematosus (SLE) is a complex disease involving many interactions at the molecular level, the details of which remain unclear. Here, we demonstrated an analytical paradigm of prioritizing genes and regulatory elements based on GWAS loci at the single-cell levels. Our initial step was to apply TWMR to identify causal genes and causal methylation sites in SLE. Based on the eQTL, LD and mQTL, we calculated the correlation between these genes and methylation sites. Next, we separately used gene expression and DNAm as exposure variables and outcome variables to analyze the regulatory mechanisms. We identified two mediating modes for SLE: 1) transcription mediation model and 2) epigenetic mediation model. Further, using single-cell RNA sequencing data, we revealed the cell subclusters associated with these mechanisms. Our identification of the mechanisms of SLE in different cell populations is of great significance for understanding the heterogeneity of disease in different cell populations.

5.
Bioresour Technol ; 361: 127707, 2022 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-35905871

RESUMO

Confronting the complex contaminated water, Aquabacterium sp. CZ3 could perform microbially induced calcium precipitation (MICP) under facultative anaerobic condition using phenol as supplementary carbon source. Strain CZ3 exhibited a remarkable ability to remove nitrate, fluoride, calcium and phenol with removal rates of 100.00, 87.50, 66.24 and 100.00%, respectively. The Modified Gompertz model was used for kinetic analysis to determine the optimum conditions for denitrification and degradation of phenol. The mechanism of anaerobic MICP was enhanced by measuring the self-aggregation properties of the isolates. The mechanism of fluoride removal was identified as co-precipitation and adsorption by characterization analysis of the bioprecipitation. Furthermore, the changes in soluble metabolites under phenol stress explained the utilization of phenol as a co-substrate by microorganisms. This is a novel report on phenol degradation by anaerobic MICP, which provides a theoretical basis for expanding its practical application.

6.
J Adv Res ; 39: 275-289, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35777912

RESUMO

INTRODUCTION: The principal voltage-gated Na+ channel, NaV1.5 governs heart excitability and conduction. NaV1.5 dysregulation is responsible for ventricular arrhythmias and subsequent sudden cardiac death (SCD) in post-infarct hearts. The transcription factor Meis1 performs a significant role in determining differentiation fate and regenerative capability of cardiomyocytes. However, the functions of Meis1 in ischemic arrhythmias following myocardial infarction (MI) are still largely undefined. OBJECTIVES: Here we aimed to study whether Meis1 could act as a key regulator to mediate cardiac Na+ channel and its underlying mechanisms. METHODS: Heart-specific Meis1 overexpression was established by AAV9 virus injection in C57BL/6 mice. The QRS duration, the incidence of ventricular arrhythmias and cardiac conduction velocity were evaluated by ECG, programmed electrical stimulation and optical mapping techniques respectively. The conventional patch clamp technique was performed to explore the INa characteristics of isolated mouse ventricular myocytes. In vitro, Meis1 was also overexpressed in hypoxic-treated neonatal cardiomyocytes. The analysis of immunoblotting and immunofluorescence were used to detect the changes in the expression of NaV1.5 in each group. RESULTS: We found that forced expression of Meis1 rescued the prolongation of QRS complex, produced anti-arrhythmic activity and improved epicardial conduction velocity in infarcted mouse hearts. In terms of mechanisms, cardiac electrophysiological changes of MI mice can be ameliorated by the recovery of Meis1, which is characterized by the restoration of INa current density and NaV1.5 expression level of cardiomyocytes in the marginal zone of MI mouse hearts. Furthermore, in vitro studies showed that Meis1 was also able to rescue hypoxia-induced decreased expression and dysfunction of NaV1.5 in ventricular myocytes. We further revealed that E3 ubiquitin ligase CDC20 led to the ubiquitination and degradation of Meis1, which blocked the transcriptional regulation of SCN5A by Meis1 and ultimately led to the electrophysiological remodeling in ischemic-hypoxic cardiomyocytes. CONCLUSION: CDC20 mediates ubiquitination of Meis1 to govern the transcription of SCN5A and cardiac electrical conduction in mouse cardiomyocytes. This finding uncovers a new mechanism of NaV1.5 dysregulation in infarcted heart, and provides new therapeutic strategies for malignant arrhythmias and sudden cardiac death following MI.


Assuntos
Proteína Meis1 , Infarto do Miocárdio , Fatores de Transcrição , Animais , Arritmias Cardíacas , Morte Súbita Cardíaca , Camundongos , Camundongos Endogâmicos C57BL , Proteína Meis1/genética , Proteína Meis1/metabolismo , Infarto do Miocárdio/metabolismo , Miócitos Cardíacos/metabolismo , Fatores de Transcrição/metabolismo
7.
J Hazard Mater ; 435: 129086, 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35650733

RESUMO

Black phosphorus (BP), one rising star of two-dimensional (2D) materials, has showcased a huge capability for ppb-level NO2 detection. However, sluggish reaction kinetics and fragile stability frustrate its further application. In this regard, for the first time we prepared Ag nanoparticles modified BP nanosheets as the sensing layer via one feasible method to recognize trace NO2 at room temperature. With respect to individual BP, the composition-optimized BP-Ag nanocomposites (BP-Ag-1 sensor) achieved a favorable performance primarily in terms of boosted response (39.9% vs. 11.8%, 100 ppb NO2), accelerated response speed (190 s vs. 486 s, 100 ppb NO2) and strengthened operation stability, together with ultralow theoretical detection limit of 0.25 ppb. Furthermore, a protection layer comprised of polylactic acid (PLA) was anchored onto the surface of BP-Ag-1 sensor to keep the water molecules physically from the sensing layer and retain a distinguishable signal toward trace NO2 at high moisture environments. The introduction of Ag and PLA separately reduced the lone electron pairs from P atoms and suppressed the water penetration into the BP film, thereby offering an alternative way to passivate BP for its optoelectronic applications in the future.

8.
Front Genet ; 13: 891665, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35711928

RESUMO

Tumor metastasis and invasion are the main impediments to lung adenocarcinoma successful treatment. Previous studies demonstrate that chemotherapeutic agents can elevate the malignancy of cancer cells other than their therapeutic effects. In this study, the effects of transient low-dose cisplatin treatment on the malignant development of lung adenocarcinoma cells (A549) were detected, and the underlying epigenetic mechanisms were investigated. The findings showed that A549 cells exhibited epithelial-mesenchymal transition (EMT)-like phenotype along with malignant progression under the transient low-dose cisplatin treatment. Meanwhile, low-dose cisplatin was found to induce contactin-1 (CNTN-1) upregulation in A549 cells. Subsequently, we found that further overexpressing CNTN-1 in A549 cells obviously activated the EMT process in vitro and in vivo, and caused malignant development of A549 cells in vitro. Taken together, we conclude that low-dose cisplatin can activate the EMT process and resulting malignant progression through upregulating CNTN-1 in A549 cells. The findings provided new evidence that a low concentration of chemotherapeutic agents could facilitate the malignancy of carcinoma cells via activating the EMT process other than their therapeutic effects.

9.
Cancer Cell Int ; 22(1): 214, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35706026

RESUMO

BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is the leading cause of cancer-related death worldwide with a poor prognosis. Given that DEPDC1B plays a key role in multiple cancers, the role of this molecule in ESCC was explored to identify potential targets for ESCC patients. METHOD: The expression level of DEPDC1B in ESCC was revealed based on the TCGA database and immunohistochemical experiments on clinical tissues. The correlation between DEPDC1B and survival of ESCC patients was analyzed by Kaplan-Meier method. Small hairpin RNA (shRNA)-mediated silencing of DEPDC1B expression in ESCC cells and performed a series of in vitro and in vivo functional validations. RESULT: DEPDC1B was overexpressed in ESCC. High expression of DEPDC1B was significantly negatively correlated with overall survival in patients with ESCC. Moreover, knockdown of DEPDC1B inhibited ESCC cell proliferation, clone formation, migration, tumor formation and promoted apoptosis. Furthermore, knockdown of DEPDC1B leaded to significant downregulation of GABRD in ESCC cells. Meanwhile, GABRD expression was upregulated in ESCC, and its silencing can inhibit the proliferation and migration of the tumor cells. Interestingly, there was a protein interaction between DEPDC1B and GABRD. Functionally, GABRD knockdown partially reversed the contribution of DEPDC1B to ESCC progression. In addition, GABRD regulated ESCC progression may depend on PI3K/AKT/mTOR signaling pathway. CONCLUSION: DEPDC1B collaborated with GABRD to regulate ESCC progression, and inhibition of this signaling axis may be a potential therapeutic target for ESCC.

10.
Int J Mol Sci ; 23(12)2022 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-35742832

RESUMO

Chayote (Sechium edule) produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. 'Tuershao', a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization.


Assuntos
Cucurbitaceae , Solanum tuberosum , Cucurbitaceae/genética , Regulação da Expressão Gênica de Plantas , Metaboloma , Tubérculos/metabolismo , Solanum tuberosum/metabolismo , Amido/metabolismo , Transcriptoma
11.
Ecotoxicol Environ Saf ; 241: 113787, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35738104

RESUMO

The wide presence of antibiotics and minerals warrants their combined effects on the denitrification in natural aquatic environment. Herein, we investigated the effects of two antibiotics, sulfamethazine (SMZ) and chlortetracycline (CTC), on the reduction of NO3--N and accumulation of NO2--N in the absence and presence of engineered nanoparticles (NPs) (Al2O3, SiO2, and geothite) using 16 S rRNA sequencing and high-throughput quantitative PCR. The results showed that the addition of antibiotics inhibited the reduction of NO3--N by changing the bacterial community structure and reducing the abundance of denitrification genes, while engineered NPs promoted the denitrification by increasing the abundance of denitrification genes. In the binary systems, engineered NPs alleviated the inhibitory effect of antibiotics through enriching the denitrification genes and adsorbing antibiotics. Antibiotics and its combination with engineered NPs changed the composition of functional genes related to C, N, P, S metabolisms (p < 0.01). The addition of antibiotics and/or engineered NPs altered the bacterial community structure, which is dominated by the genera of Enterobacter (40.7-90.5%), Bacillus (4.9-58.5%), and Pseudomonas (0.21-12.7%). The significant relationship between denitrification, carbon metabolism genes, and antibiotic resistance genes revealed that the heterotrophic denitrifying bacteria may host the antibiotic resistance genes and denitrification genes simultaneously. The findings underscore the significance of engineered NPs in the toxicity assessment of pollutants, and provide a more realistic insight into the toxicity of antibiotics in the natural aquatic environment.


Assuntos
Desnitrificação , Nanopartículas , Antibacterianos/farmacologia , Resistência Microbiana a Medicamentos , Nitrogênio/metabolismo , Dióxido de Silício/farmacologia
12.
Biochem Pharmacol ; 202: 115139, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35697119

RESUMO

Therapeutically targeting B cells has received great attention in the treatment of B-cell malignancies and autoimmune diseases. The B-cell activating factor (BAFF) is critical to the survival of normal and neoplastic B cells, and excess production of BAFF contributes to autoimmune diseases. Resveratrol, a natural polyphenolic compound, has a positive effect on the treatment of autoimmune diseases. However, how resveratrol affects BAFF-stimulated B-cell proliferation and survival is poorly understood. Here, we show that resveratrol increased autophagosome formation and ATG5/LC3-II levels and decreased p62 level, promoting autophagic flux/autophagy and thereby suppressing the basal or human soluble BAFF (hsBAFF)-stimulated proliferation and survival of normal and B-lymphoid (Raji) cells. This is supported by the findings that inhibition of autophagy with 3-methyladenine (3-MA, an inhibitor of Vps34) or ATG5 shRNA attenuates resveratrol-induced autophagy and -reduced proliferation/viability in B-cells. Inhibition of mTOR with rapamycin or knockdown of mTOR potentiated resveratrol-induced autophagy and inhibition of hsBAFF-stimulated B-cell proliferation/viability, while overexpression of wild-type mTOR conferred resistance to the actions of resveratrol. Similarly, inhibition of Akt with Akt inhibitor X or ectopic expression of dominant negative Akt reinforced resveratrol-induced autophagy and inhibition of hsBAFF-stimulated B-cell proliferation/viability, whereas expression of constitutively active Akt conferred resistance to the actions of resveratrol. Taken together, these results indicate that resveratrol induces autophagy impeding BAFF-stimulated proliferation and survival via blocking the Akt/mTOR signaling pathway in normal and neoplastic B cells. Our findings highlight that resveratrol has a great potential for prevention and treatment of excessive BAFF-elicited aggressive B-cell disorders and autoimmune diseases.


Assuntos
Doenças Autoimunes , Fator Ativador de Células B , Apoptose , Autofagia , Fator Ativador de Células B/genética , Fator Ativador de Células B/metabolismo , Fator Ativador de Células B/farmacologia , Proliferação de Células , Sobrevivência Celular , Humanos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Resveratrol/farmacologia , Serina-Treonina Quinases TOR/metabolismo
13.
Carbohydr Polym ; 291: 119554, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35698382

RESUMO

Efficient delivery systems for co-delivery of P-glycoprotein (P-gp) inhibitors and chemotherapeutic drugs are essential for inhibiting multi-drug resistance (MDR) breast cancers. Herein, we present a multi-functional carboxymethyl chitosan (CMC) based core-shell nanoplatform to co-deliver MDR1 gene-silenced small interfering RNA (siMDR1) and doxorubicin (DOX) for optimal combinatorial therapy. DOX is linked to CMC through a disulfide bond to model redox-responsive prodrug (CMC-DOX) as the inner core. siMDR1 is encapsulated in oligoethylenimine (OEI), which is electrostatically adsorbed on CMC-DOX as the pH-responsive sheddable shielding shell. AS1411 aptamer and GALA peptide functionalised hyaluronic acid (AHA/GHA) are provided on the surface for tumour-targeting and endo/lysosomal escape. The nanoplatform could stepwise release payloads with acid/redox triggered fashion. AHA effectively improves nanoplatform intracellular uptake and tumour accumulation. GHA facilitates cargos escape from endo/lysosomes to cytoplasm. The multi-functional nanoplatform provides 86.3 ± 2.2% siMDR1 gene silencing and significantly downregulates P-gp expression. Moreover, it ensures 55.7 ± 1.6% MCF-7/ADR cell apoptosis at a low concentration of DOX (30 µg/mL) in vitro and performs synergistic therapeutic effects suppressing tumour growth in vivo. Overall, the multi-functional CMC-based biopolymers can be efficient siRNA/drug co-delivery carriers for cancer chemotherapy.


Assuntos
Neoplasias da Mama , Quitosana , Nanopartículas , Neoplasias da Mama/patologia , Quitosana/farmacologia , Doxorrubicina , Portadores de Fármacos/química , Resistencia a Medicamentos Antineoplásicos , Feminino , Humanos , Células MCF-7 , Nanopartículas/química , RNA Interferente Pequeno/química
14.
J Hazard Mater ; 436: 129214, 2022 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-35739736

RESUMO

As emerging pollutants, the environmental geochemistry of organophosphate esters (OPEs) in the coastal zone with multiple functional areas are still less recognized. This study investigated spatiotemporal distribution, sources and risks of 11 widely used OPEs in surface waters from seagoing rivers and multiple coastal functional areas of the Beibu Gulf. The results indicated that significantly higher ∑11OPEs (total concentrations of 11 OPEs, ng/L) occurred in summer (34.2-1227) than in winter (20.6-840), as a result of the high emission caused by climate reasons. In general, higher ∑11OPEs occurred in rivers (41.2-1227) than in the coast (34.2-809) in summer, especially in the urban rivers, while in winter, higher ∑11OPEs occurred in the coast (23.4-840 vs 20.6-319 in rivers) because of obviously higher ∑11OPEs in marine fishery areas (99-840). Source identification revealed that fishery activity, especially fishing vessels, and urban rivers were the main sources of OPEs in the Beibu Gulf. For the individual OPE, only tri-n-butyl phosphate (TNBP) may have ecological risks to aquatic organisms in a few sites, but if considering the additive effects, the OPEs mixtures would pose a high risk to algae and low to medium threats to crustaceans and fish.


Assuntos
Retardadores de Chama , Poluentes Químicos da Água , Animais , China , Monitoramento Ambiental/métodos , Ésteres , Pesqueiros , Retardadores de Chama/análise , Organofosfatos , Medição de Risco , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
15.
Front Nutr ; 9: 874943, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35634408

RESUMO

Objective: The purpose of this study was to evaluate the associations of serum biomarkers of fruit and vegetable intake (vitamin C and carotenoids) with cause-specific mortality and all-cause mortality in a nationally representative sample of US adults. Methods: We analyzed data from 12,530 participants from the National Health and Nutrition Examination Survey III (1988-1994). The Cox proportional hazards models with restricted cubic spline were used for the analysis. Results: During 246,027 person-years of follow-up, 4,511 deaths occurred, including 1,395 deaths from cardiovascular disease, 1,072 deaths from heart disease, 323 deaths from cerebral disease, and 954 deaths from cancer. The serum vitamin C was significantly associated with the cancer and all-cause mortality, with hazard ratios (HRs) (95% CIs) for each one SD of 0.80 (0.71-0.91) and 0.91 (0.86-0.96). The serum alpha-carotene was significantly associated with the cancer mortality, with HRs (95% CIs) of 0.70 (0.54-0.90), 0.68 (0.48-0.95), 0.64 (0.43-0.95), and 0.44 (0.33-0.60) for comparisons of groups 2-5 with group 1 in model 2, respectively. The change for each one SD in the composite biomarker score, equivalent to a 0.483 times/month difference in total fruits and vegetables intake, gave an HR of 0.79 (0.69-0.90) for cancer mortality. Conclusion: Inverse associations were found between serum vitamin C, carotenoids, and composite biomarker score and outcomes expect for cerebral disease, heart disease, and cardiovascular disease mortality. This finding supports an increase in dietary fruit and vegetable intake as a primary prevention strategy for cancer and all-cause mortality.

16.
Spectrochim Acta A Mol Biomol Spectrosc ; 277: 121279, 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-35512446

RESUMO

The feasibility of laser cooling of AuF molecule and its cation are investigated from vibrational and rotational perspectives. The spectroscopy of AuF molecule and AuF+ molecular cation are obtained by the method of multireference configuration interaction plus Davidson correction (MRCI + Q) and spin-orbit coupling (SOC) effect. On account of the accurate molecular spectroscopy and the transition dipole moment, the Franck-Condon factors and radiative lifetimes of AuF molecule and AuF+ molecular cation are calculated. Comparing the criterias of laser cooling candidate molecules, the AuF is an excellent candidate for laser cooling and while AuF+ is not sutable. The b3Π0+ ↔ Χ1Σ+0+ transition of AuF is selected for laser cooling and an optical cycling scheme is proposed. The scheme possesses highly diagonally Franck-Condon factors and the scattered photons achieve âˆ¼ 104. Furthermore, the rotational transition analysis is also included in our work and found that its Franck Condon factors and Einstein coefficients are undistorted. Our work could provide theoretical support and accelerate the laser cooling of AuF molecules in experiments.

17.
Comput Math Methods Med ; 2022: 2287991, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35495885

RESUMO

The existing recognition methods of complex human movements in Wushu have the problem of imperfect kinetic energy model, which leads to low recognition accuracy. A complex human motion recognition method based on bone point features is designed. Identify martial arts movement posture, combine the upward movement of human center of gravity trajectory, establish the kinetic energy model of joints according to the positioning results of extremity points, set the threshold of local spatial differences of human bones with the central node of hip joint as the center point, avoid overcalculation, and optimize the complex motion identification process by combining the characteristics of bone points. Experimental Results. The correct rate of different types of actions identified by this method is 90.1% and 92.7%, and the identification time is 1.2 s and 1.41 s, which shows that this method can identify actions quickly and effectively by combining the feature information of bone points.


Assuntos
Artes Marciais , Movimento , Gravitação , Articulação do Quadril , Humanos , Postura
18.
Chemosphere ; 302: 134848, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35526689

RESUMO

Self-assembled fungus-flexible fiber composite microspheres (SFFMs) were firstly combined with microbially induced calcium precipitation (MICP) in a continuous-flow bioreactor and achieved the efficient removal of fluoride (F-), nitrate (NO3-), and calcium (Ca2+). Under the influent F- of 3.0 mg L-1, pH of 7.0, and HRT of 8 h, the average removal efficiencies reached 77.54%, 99.39%, and 67.25% (0.29, 2.03, and 8.34 mg L-1 h-1), respectively. Fluorescence spectrum and flow cytometry analyses indicated that F- content significantly affected the metabolism and viability of bacteria. SEM images showed that flexible fibers and intertwined hyphae provided effective locations for bacterial colonization in SFFMs. The precipitated products were characterized by XRD and FTIR, which revealed that F- was mainly removed in the form of calcium fluoride and calcium fluorophosphate (CaF2 and Ca5(PO4)3F). High-throughput analysis at different levels demonstrated that Pseudomonas sp. WZ39 acted as the core strain, which played a crucial role in the bioreactor. The mechanism of enhanced denitrification was attributed to minor F- stress and bioaugmentation technology. This study highlighted the superiorities of SFFMs and MICP combined remediation and documented a promising option for F-, NO3-, and Ca2+ removal.


Assuntos
Cálcio , Nitratos , Cálcio na Dieta , Desnitrificação , Fluoretos , Fungos , Microesferas , Óxidos de Nitrogênio
19.
Metab Brain Dis ; 2022 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-35612695

RESUMO

Ischemic stroke (IS) is the most prevalent type of stroke. The early diagnosis and prognosis of IS are crucial for successful therapy and early intervention. Metabolomics, a tool in systems biology based on several innovative technologies, can be used to identify disease biomarkers and unveil underlying pathophysiological processes. Accordingly, in recent years, an increasing number of studies have identified metabolites from cerebral ischemia patients and animal models that could improve the diagnosis of IS and prediction of its outcome. In this paper, metabolomic research is comprehensively reviewed with a focus on describing the metabolic changes and related pathways associated with IS. Most clinical studies use biofluids (e.g., blood or plasma) because their collection is minimally invasive and they are ideal for analyzing changes in metabolites in patients of IS. We review the application of animal models in metabolomic analyses aimed at investigating potential mechanisms of IS and developing novel therapeutic approaches. In addition, this review presents the strengths and limitations of current metabolomic studies on IS, providing a reference for future related studies.

20.
Small ; 18(22): e2201719, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35506200

RESUMO

Na3 V2 (PO4 )2 F3 has attracted wide attention due to its high voltage platform, and stable crystal structure. However, its application is limited by the low electronic conductivity and the ease formation of impurity. In this paper, the spherical Br-doped Na3 V2 (PO4 )2 F3 /C is successfully obtained by a one-step spray drying technology. The hard template polytetrafluoroethylene (PTFE) supplements the loss of fluorine, forming porous structure that accelerates the infiltration of electrolyte. The soft template cetyltrimethylammonium bromide (CTAB) enables doping of bromine and can also control the fluorine content, meanwhile, the self-assembly effect strengthens the structure and refines the size of spherical particles. The loss, compensation, and regulation mechanism of fluorine are investigated. The Br-doped Na3 V2 (PO4 )2 F3 /C sphere exhibits superior rate capability with the capacities of 116.1, 105.1, and 95.2 mAh g-1 at 1, 10, and 30 C, and excellent cyclic performance with 98.3% capacity retention after 1000 cycles at 10 C. The density functional theory (DFT) calculation shows weakened charge localization and enhanced conductivity, meanwhile the diffusion energy barrier of sodium ions is reduced with Br doping. This paper proposes a strategy to construct fluorine-containing polyanions cathode, which enables the precise regulation of structure and morphology, thus leading to superior electrochemical performance.

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