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1.
J Hazard Mater ; 412: 125214, 2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33529835

RESUMO

Our previous study revealed PAHs' wide occurrence in corals from multiple coral reef regions (CRRs) in the South China Sea. However, little is known about their occurrence, distribution, fate, and sources in the ambient environment of these CRRs. This study aimed to resolve these research gaps. The results showed ∑15PAHs (total concentrations of 15 US EPA priority controlled PAHs exclude naphthalene) in the atmosphere (gas-phase: 0.31-49.6 ng m-3; particle-phase: 2.6-649 pg m-3) were mainly influenced by air mass origins. Southwesterly wind caused higher ∑15PAHs than the southeasterly wind. The ∑15PAHs in seawater from the nearshore (462 ± 244 ng L-1) was higher than that from offshore Zhongsha Islands (80.5 ± 72.1 ng L-1) because of the effect of terrigenous pollution and ocean current. Source apportionment indicated that the mixed sources of spilled oil and combustion from neighboring countries were the main contributors to PAHs in these CRRs. The total deposition fluxes showed that PAHs tended to migrate from the atmosphere to seawater. Global warming may inhibit this process, but PAHs still have a migration pattern of atmosphere-ocean-corals, which will further increase the environmental pressure on coral reef ecology.

2.
Plant Cell ; 2021 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-33582814

RESUMO

In wheat (Triticum aestivum L.), breeding efforts have focused intensively on improving grain yield and quality. For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality. Moreover, starch levels in seeds are associated with yield. However, little is known about the mechanisms that coordinate SSP and starch accumulation in wheat. In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation. TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes. Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters. TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb. TaNAC019 also indirectly regulated the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation. Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality. Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.

3.
Free Radic Biol Med ; 166: 1-10, 2021 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-33588051

RESUMO

Lithium chloride (LiCl), a pharmacological compound, was effective in reducing inflammation, but whether it can protect against abdominal aortic aneurysm (AAA) is largely unknown. This study is designed to investigate therapeutic effects of LiCl on AAA and the potential mechanism. Rat AAA models were induced by periaortic application of CaCl2. AAA rats were treated by daily intraperitoneal injection of LiCl or vehicle alone to study the protection effects of LiCl in vivo. Rat primary vascular smooth muscle cells (VSMCs) stimulated with tumor necrosis factor (TNF)-α served as an in vitro model. LiCl treatment prevented the development of AAA through inhibiting the inflammatory cells infiltration and inflammatory cytokines overproduction, as well as attenuating superoxide production and elastin degradation in aorta of AAA rats. Additionally, the downregulation of p-GSK3ß(Ser9) and SIRT1, upregulation of NF-κB(p-65), MMP-2 and MMP-9 in AAA were abolished by LiCl treatment. In vitro by upregulating p-GSK3ß(Ser9), LiCl significantly induced SIRT1 expression, along with inhibition of the NF-κB activation and decreased elastin level elicited in VSMCs by TNF-α stimulation. SIRT1 activator SRT1720 achieved similar repressive effects as LiCl on TNF-α-induced NF-κB activation and decreased elastin in VSMCs. Moreover, administration of LiCl also caused regression of established rats AAA. This study provided the first evidence that LiCl prevented the development of AAA through inhibiting inflammation, MMPs, and superoxide production, and facilitating the biosynthesis of elastin. The beneficial effect of LiCl may be mediated by regulation GSK3ß/SIRT1/NF-κB cascade.

4.
Mol Genet Genomics ; 296(2): 423-435, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33507382

RESUMO

Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system, and the pathogenesis is influenced by genetic susceptibility. Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) play essential roles in complex diseases, including acting as competing endogenous RNAs (ceRNAs). However, the functional roles and regulatory mechanisms of lncRNAs acting as ceRNAs in MS are still unclear. In this study, we identified hub lncRNA ceRNAs in MS based on ceRNA mechanisms and annotated their functions. The lncRNA-associated ceRNA network (LACN) was constructed by integrating the expression profiles of lncRNA/mRNA and miRNA in MS and normal samples, and the experimentally validated interactions of lncRNA-miRNA and mRNA-miRNA. We found three hub lncRNA ceRNAs (XIST, OIP5-AS1, and CTB-89H12.4) using the network analysis and obtained 96 lncRNA-mediated competing triplets (LCTs, lncRNA-miRNA-mRNA) with the hub lncRNA ceRNAs, which constituted 3 hub ceRNA modules. The functional analysis identified 12 pathways enriched by the 3 hub lncRNA ceRNAs, of which 6 were confirmed to be related to MS. For example, XIST was enriched in the 'spliceosome' and 'RNA transport' related to the typing of MS, and CTB-89H12.4 was enriched in the 'mTOR signaling pathway,' a potential therapeutic target for MS. We dissected the expression patterns of the 96 LCTs in MS individually. LCT XIST-miR-326-HNRNPA1, for which the expression pattern in MS revealed that XIST and HNRNPA1 were up-regulated and miR-326 was down-regulated, consisted of risk RNAs for MS that were validated by other research. Therefore, XIST-miR-326-HNRNPA1 might play a central role in the pathogenesis of MS. These results will contribute to the discovery of novel biomarkers and the development of new therapeutic methods for MS.


Assuntos
Ribonucleoproteína Nuclear Heterogênea A1/genética , MicroRNAs/genética , Esclerose Múltipla/genética , RNA Longo não Codificante/genética , Biomarcadores Tumorais/genética , Bases de Dados Genéticas , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Redes Reguladoras de Genes , Predisposição Genética para Doença , Humanos , Anotação de Sequência Molecular , RNA Mensageiro/genética
5.
Chemosphere ; 271: 129589, 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33453486

RESUMO

The epigenetic regulation mechanisms for toxicity induction of nanoplastics in organisms remain largely unknown. In Caenorhabditis elegans, we found that prolonged exposure to 1-100 µg/L polystyrene nanoparticles (PS-NPs) decreased expression of MET-2, a H3K9 methyltransferase. Meanwhile, RNAi knockdown of met-2 suppressed the PS-NPs toxicity in inducing production of reactive oxygen species (ROS) and in decreasing locomotion behavior, which suggesting that the decrease in MET-2 expression reflected a protective response. This resistance to PS-NPs toxicity could be further detected in worms with met-2 RNAi knockdown in both intestinal cells and germline cells. In PS-NPs exposed worms, intestinal RNAi knockdown of met-2 significantly increased expressions of daf-16, bar-1, and elt-2. Intestinal RNAi knockdown of daf-16, bar-1, or elt-2 suppressed the resistance of met-2(RNAi) worms to PS-NPs toxicity, suggesting that MET-2 functioned upstream of ELT-2, BAR-1, and DAF-16 in intestinal cells to control PS-NPs toxicity. Moreover, in PS-NPs exposed worms, germline RNAi knockdown of met-2 significantly decreased expressions of wrt-3 and pat-12. RNAi knockdown of wrt-3 or pat-12 further inhibited the susceptibility of worms overexpressing germline MET-2 to PS-NPs toxicity, suggesting that MET-2 functioned upstream of PAT-12 and WRT-3 in germline cells to control PS-NPs toxicity. Therefore, our data provided an important molecular basis for MET-2-mediated methylation regulation in causing protective response to nanoplastics in organisms.

6.
Sci Total Environ ; 752: 141882, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32889286

RESUMO

Antibiotic residues in mariculture environments have been detected globally, while little information is available about their dynamic levels, source, behavior, and fate during the whole culture process. In this study, the dynamic occurrence, bioaccumulation, source, fate, and human dietary risk of 19 antibiotics were investigated in different breeding stages of mariculture ponds near the Maowei Sea, South China. Fourteen antibiotics, including three sulfonamides (SAs), five fluoroquinolones (FQs), three macrolides (MLs), and two chloramphenicols (CAPs), were detected in the mariculture ponds, with FQs being the most abundant antibiotics. Significant variations of antibiotic concentration occurred during the whole culture process. Severe weather, especially typhoons and rainstorms, resulted in the average highest levels of ∑19antibiotics (mean: 567 ng L-1) in mariculture ponds. The source apportionment estimated for the mariculture ponds showed that direct application was the primary source of antibiotics (91.2%). The antibiotics in mariculture ponds were mainly discharged through aquaculture wastewater (65.8%) and settling particles (33.8%). The estimated annual input of antibiotics into the Maowei Sea was 2.24 times higher through the two main rivers (48.0 kg a-1) than through the mariculture wastewater (24.1 kg a-1). The apparent bioaccumulation factors (ABAFs) confirmed that young and adult tilapia accumulated more sulfamethoxazole (SMX) and norfloxacin (NOX), respectively. The result from the estimated daily intakes suggested that the antibiotics in the seafood could not pose a risk to human health by dietary exposure assessment. CAPSULE: Big variation of antibiotic concentration occurred during the whole culture process in the mariculture farms, and the storm increased antibiotic application.


Assuntos
Tanques , Poluentes Químicos da Água , Antibacterianos/análise , China , Monitoramento Ambiental , Humanos , Poluentes Químicos da Água/análise
7.
J Int Med Res ; 48(12): 300060520976824, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33284728

RESUMO

Ovarian cancer remains the most lethal gynecological malignant tumor, with relapse occurring in approximately 70% of advanced cases. Anlotinib is an oral small-molecule multi-targeted tyrosine kinase inhibitor that can resist neoangiogenesis and inhibit tumor growth. Previous research demonstrated clinical antitumor activity of anlotinib in various cancers. We report the case of an elderly woman with advanced ovarian cancer who received anlotinib after failure of multiple-line chemotherapy. A partial response was observed after six cycles of anlotinib monotherapy, with a reduction in the size of the metastases and significantly decreased serum CA125 levels from 1832.7 U/mL to 118.7 U/mL. She continued to take anlotinib, with a progression-free survival time of more than 4 months. Only mild hypertension was observed during the treatment. Anlotinib monotherapy may be a novel therapeutic option for patients with advanced ovarian cancer.

8.
Environ Pollut ; 267: 115675, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33254612

RESUMO

Occurrence, behavior, and fate of 11 OPEs in multiple environmental matrices, which include air, rainwater, dustfall, paddy soil, irrigation water, and rice plants from nine subtropical paddy fields of South China, were investigated. The total concentrations of 11 OPEs (∑11OPEs) in all matrices are generally higher in the urban areas than in rural areas, and they are higher in summer than in fall. However, both urban and rural areas showed a similar composition profile of OPEs, indicating that the OPEs come from similar sources in the two areas. Except for irrigation water, significant positive correlations of ∑11OPEs were observed between air and the other five matrices. The exchange and partition of OPEs among air, soil, and water demonstrate that most of OPEs were transferred from air into water and soil, and from water into soil. Thus, the air may be an important source of OPEs in the paddy fields, and the soil may act as a principal environmental reservoir of OPEs. The contribution of air-soil exchange, atmospheric deposition (rainwater plus dustfall), and irrigation water to the total input fluxes of OPEs (2100 ± 980 ng/m2/day) reached an average of 19%, 38% (37% + 1%), and 43%, respectively. The water (rainwater plus irrigation water) is the primary medium transferring the OPEs into the paddy fields and contributed to the input flux by 80%. Output flux of OPEs via mature rice plants was about 220 µg/m2, 2% of which were presented in rice, and the remaining 98% may be re-released into the environment through the pathway of straw turnover or burning. Dietary exposure via rice was much higher than inhalation exposure, dust ingestion, and dermal absorption via dust. However, no data shows that all of the intakes via the four exposure pathways could cause the risks to human health at present.

9.
J Glob Health ; 10(2): 020436, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33312503

RESUMO

Background: Poor personal hygiene increases disease risk, however, the prevalence of hygiene practices among adolescents is poorly described in low- and middle-income countries (LMICs). We aimed to assess the hygiene practices among young adolescents in LMICs using data from the Global School-based Student Health Surveys (GSHS). Methods: This population-based study analysed the GSHS data for adolescents aged 12-15 years from 75 LMICs. Data were collected between 2003 and 2015 using standardised, anonymous, self-reported questionnaires. This report focuses on hygiene related behaviours such as tooth brushing, washing hands after using the toilet, washing hands before eating and washing hands with soap. The weighted prevalence and 95% confidential intervals (CIs) for the hygiene practices, and overall and regional estimates were calculated with random-effects meta-analysis. Results: A total of 181 848 young adolescents from 75 LIMCs with available hygiene data were analysed. The overall prevalence for never washing hands were 7.4% (95% confidence interval (CI) = 4.4-10.3) for before eating, 5.9% (95% CI = 3.8-7.9) for after using the toilet, and 9.0% (95% CI = 6.2-11.8) for with soap. For tooth brushing, the overall prevalence estimates were 8.6% (95% CI = 5.5-11.7) for never brushing teeth, 80.9% (95% CI = 74.7-87.1) for 1-3 times per day, and 9.7% (95% CI = 5.8-13.6) for >3 times per day. However, the prevalence of different hygiene practices varied significantly among countries and regions (all P values <0.001). Poor hygiene status, with a prevalence >20%, was still observed in several LMICs (eg, 12 countries for never washing hands and 4 for never brushing teeth). Conclusions: The hygiene practices of young adolescents aged 12-15 years were generally frequent, but poor status was still observed in several LMICs. These findings emphases the need for hygiene and health education targeting young adolescents in LMICs.

10.
Eur J Med Res ; 25(1): 57, 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33176875

RESUMO

BACKGROUND: This article reports a patient who survived severe pneumonia, pyopneumothorax with acute respiratory distress syndrome (ARDS), and septic shock, which is very difficult to treat. CASE PRESENTATION: Antibiotics, continuous renal replacement therapy (CRRT), bronchial lavage and other treatments were used to treat a patient with pneumonia, pyopneumothorax, severe ARDS and septic shock. After comprehensive treatment, the patient was successfully treated and survived for a long time. CONCLUSIONS: There is a low successful clinical treatment rate for patients with pneumonia, pyopneumothorax with severe ARDS and septic shock. The successful treatment of this patient benefited from early and effective empirical therapy, targeted drug selection in the later stage, adequate closed thoracic drainage, repeated bronchial lavage, early CRRT, an appropriate respiratory support mode and parameter setting, immunotherapy and nutritional support therapy. This paper proposes a reference diagnosis and treatment solution for similar cases.

11.
Sci Rep ; 10(1): 20054, 2020 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-33208822

RESUMO

There is a dearth of information on the occurrence and risks of antibiotics in the urban rivers from plateau areas. This study investigated 83 antibiotics in water and sediments of an urban river and effluents of sewage treatment plants (E-STPs) in Xining, Qinghai (northeastern Tibetan Plateau). Fifty-three antibiotics were detected, and the concentrations of individual antibiotics varied in the range of undetected (ND)-552 ng/L in water, ND-164 ng/g in sediments, and ND-3821 ng/L in E-STPs. Seasonal differences of antibiotic concentrations were significant for water samples (p < 0.05) but insignificant for sediments (p > 0.05). In urban area, E-STP is the main source of antibiotics in the river, while runoff from manured cropland contributes partially to antibiotics in the river in the suburban area. The antibiotic compositions in water were different from those in sediments, but were similar to those in E-STPs. Notably, because of strong solar radiation and long sunshine hours in the plateau area, low levels of quinolones, which are sensitive to photolysis, were observed in river water. Moreover, norfloxacin and enrofloxacin, observed in urban river from other regions of China, were not detected in the Huangshui River water. The occurrence of ofloxacin, erythromycin, roxithromycin, clarithromycin, and trimethoprim in E-STPs may induce a possible risk to antibiotic resistance evolution. Trimethoprim, anhydroerythromycin, sulfamethoxazole, sulfapyridine, and clindamycin in river water could pose low to medium risks to aquatic organisms. Further investigation on the occurrence and distribution of antibiotic resistance genes in the Huangshui River is urgently needed.

12.
Artigo em Inglês | MEDLINE | ID: mdl-33083955

RESUMO

Although the production of polybrominated diphenyl ethers (PBDEs) has been phased out over the past decade worldwide, they are still potentially hazardous to the environment due to their persistence and toxicity. This study investigated the levels of 55 PBDEs in water and sediments from the Danjiangkou Reservoir, China. The levels of PBDEs were in the range of not detected (ND)-286.67 ng/L in water and ND-236.04 ng/g in sediments. BDE209 was the predominant PBDE congener and constituted 15-50% and 44-68% of the total PBDEs in water and sediments, respectively. Commercial pentaBDE products (70-5DE, DE-71) were the dominant source of tetraBDE, pentaBDE, and hexaBDE, while commercial octaBDE (79-8DE) and decaBDE (102E and 82-0DE) products were the main sources of nonaBDE and decaBDE in water. PBDEs in sediments mainly stemmed from commercial decaBDE products and combustion sources. BDE-209 posed high ecological risks to aquatic organisms and dominated the total ecological risks of PBDEs. No cancer risks and non-cancer risks were observed for PBDEs. A ranking method based on four criteria, i.e., detection frequency, concentration, ecological risk, and health risks, was proposed, and 17 PBDEs were identified as high priority PBDEs for future monitoring and management in the Danjiangkou Reservoir.

13.
Cell Prolif ; 53(11): e12924, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33078469

RESUMO

OBJECTIVES: Chemoresistance induced by cisplatin has become the major impediment to lung cancer chemotherapy. This study explored the potential chemoresistant genes and underlying mechanisms of chemoresistance in NSCLC. MATERIALS AND METHODS: Gene expression profile was integrated with DNA methylation profile to screen the candidate chemoresistant genes. Bioinformatic analysis and immunohistochemistry were used to analyse the association of a candidate gene with the characteristics of NSCLC patients. Recombinant lentivirus vectors were utilized to overexpress or silence candidate gene. Microarrays and immunoblotting were applied to explore the downstream targets of candidate gene. Xenograft models were established to validate the findings in vitro. RESULTS: An increased ZNF300 expression was detected in three chemoresistant cell lines of NSCLC, and the higher expression of ZNF300 was associated with poor OS of NSCLC patients. Cells with upregulated ZNF300 presented chemoresistance and enhanced aggressive growth compared to cells with downregulated ZNF300. ZNF300 inhibited MAPK/ERK pathways and activated CDK1 through inhibiting WEE1 and MYT1 and modulating MYC/AURKA/BORA/PLK1 axis. ICA and ATRA improved the anti-tumour effect of cisplatin on chemoresistant cells by inducing differentiation. CONCLUSIONS: ZNF300 promotes chemoresistance and aggressive behaviour of NSCLC through regulation of proliferation and differentiation by downregulating MAPK/ERK pathways and regulation of slow-cycling phenotype via activating CDK1 by inhibiting WEE1/MYT1 and modulating MYC/AURKA/BORA/PLK1 axis. Cisplatin, combined with ATRA and ICA, might be beneficial in chemoresistant cases of NSCLC.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Resistencia a Medicamentos Antineoplásicos , Neoplasias Pulmonares/genética , Proteínas Repressoras/genética , Transcriptoma , Animais , Antineoplásicos/farmacologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cisplatino/farmacologia , Metilação de DNA/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Masculino , Camundongos Endogâmicos BALB C , Pessoa de Meia-Idade , Proteínas Repressoras/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transcriptoma/efeitos dos fármacos
14.
Sci Rep ; 10(1): 15179, 2020 09 16.
Artigo em Inglês | MEDLINE | ID: mdl-32938983

RESUMO

Seventy-five wild tilapia samples from six rivers (ten sites) in Guangxi province were collected and analyzed for 53 organochlorine compounds. DDTs, endosulfan, and PCBs were the most dominant compounds found in this study. Tiandong County (TD) and Guigang City (GG) sites were found to be heavily contaminated with high levels of endosulfan (385-925 ng/g lw) and/or DDTs (20.1-422 ng/g lw). The diagnostic ratios indicated that the residues of DDTs and endosulfan in wild tilapia are associated with historical applications as well as the recent introduction of technical DDTs and endosulfan at some sampling sites. The correlation between total length, body mass, and organochlorines (OCs) was higher than the correlation between age and lipid content. There was no significant correlation between organochlorine pesticides (OCPs) and lipid content. Therefore, for organisms, the feeding intensity (related to length and mass) of fish could better reflect degree of pollution than exposure time (age) of fish. The hazardous ratios for the 50th and 95th percentile data of OCPs and PCBs in fish were both below 1, suggesting that daily exposure to OCPs and PCBs yields a lifetime cancer risk lower than 1 in 10,000.

15.
Sci Total Environ ; 745: 141047, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-32758726

RESUMO

TGF-ß signaling pathway is important for the regulation of stress response in organisms. We here used Caenorhabditis elegans to determine the function of DBL-1/TGF-ß signaling pathway in the control of response to nanopolystyrene (100 nm). In DBL-1/TGF-ß signaling pathway, exposure to 1-1000 µg/L nanopolystyrene significantly increased the expressions of dbl-1 encoding a TGF-ß ligand, sma-6 encoding a TGF-ß receptor, sma-4 encoding a Co-Smad, and two genes (mab-31 and sma-9) encoding transcriptional factors. DBL-1 acted in the neurons to control the response to nanopolystyrene. In the neurons, the expression and the function of DBL-1 were under the control of two signaling cascades (SMOC-1-ZAG-1 and SMOC-1-ADT-2). TGF-ß receptor SMA-6 acted in the intestine to control the response to nanopolystyrene. The downstream Co-Smad/SMA-4 and two transcriptional factors (MAB-31 and SMA-9) of SMA-6 in the intestine were further identified to be required for the control of response to nanopolystyrene. In nanopolystyrene exposed nematodes, intestinal MAB-31 activated the mitochondrial Mn-SOD/SOD-3 by modulating DAF-16 activity, and intestinal SMA-9 activated the mitochondrial unfolded protein response by affecting ELT-2 activity. Therefore, the DBL-1/TGF-ß signaling pathway mediated an important neuron-intestine communication in nanopolystyrene exposed nematodes.


Assuntos
Proteínas de Caenorhabditis elegans/genética , Neuropeptídeos , Animais , Caenorhabditis elegans , Fatores de Transcrição GATA , Intestinos , Neurônios , Poliestirenos , Transdução de Sinais , Fatores de Transcrição , Fator de Crescimento Transformador beta
16.
Environ Int ; 143: 105919, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32623222

RESUMO

Environmental exposure to organophosphate esters (OPEs) continues to be a concern. Little is known about their bioaccumulation and trophodynamics, especially in tropical food webs. This study collected seawater and fifteen types of organism from a tropical ecosystem, South China Sea, to investigate the species-specific compositional, bioaccumulation, and trophic transfer of OPEs. The total concentrations of 11 target OPEs (ng/g dw) in the organisms decreased with the increase of their trophic levels in the order: phytoplankton (922) > zooplankton (660) > oysters (309) > crabs (225) > coral tissues (202) > fishes (58.2). The composition profiles (relative abundances) of OPEs were different among the species of organisms, which is likely affected by metabolism and the physicochemical property of OPEs. The trophic biomagnification of tripentyl phosphate (TPTP) in the pelagic food web was unexpected and requires further investigation. The trophic magnification factors (TMFs) of OPEs were generally lower in this tropical aquatic food web than in temperate and frigid aquatic food web. Our analysis suggests that there is a significant positive linear correlation between latitude and TMF. Intakes of OPEs through the consumption of the seafood involved in this work does not pose health risk to adults.

17.
BMC Surg ; 20(1): 162, 2020 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-32698794

RESUMO

BACKGROUND: Intraoperative pulmonary artery (PA) hemorrhage is one of the leading reasons for conversion from uniportal VATS to open thoracotomy, especially for the small incision (≤3 cm) uniportal VATS performed by our department. So, We designed a technology called pretreatment clamping of the pulmonary artery, which may be helpful to solve the problem. METHODS: A retrospective analysis of 19 patients who had pulmonary artery bleeding during uniportal thoracoscopic lobectomy in which one group had undergone preventive pulmonary artery clamping, the clamping group (n = 11), and one group which did not receive preventive clamping, the non-clamping group (n = 8). We compared the rates of conversion from the uniportal VATS approach to open thoracotomy or multi-incision operation, duration of pulmonary artery repair, blood loss, length of postoperative hospital stay and postoperative complications of the two groups. RESULTS: Compared to the non-clamping group, the clamping group had lower rates of conversion to open thoracotomy (0% vs 62.5%, p < 0.05) and lower rates of conversion to multi-incision operations (18.2% of non-clamping converted to 2-port approach vs 12.5% of clamping converted to 2-port approach and 12.5% converted to 3-port approach, p < 0.05). Duration of pulmonary artery repair was reduced in the clamping group (10.1 ± 3.2 min vs 18.3 ± 5.5 min, p < 0.05). The clamping group also had decreased blood loss (23.6 ± 11.2 ml vs 47.5 ± 14.9 ml, p<0.05). There were no significant differences in postoperative hospital stay and postoperative complications between the two groups. CONCLUSION: Pretreatment clamping of the pulmonary artery in VATS lobectomy can decrease conversion rates, decrease blood loss, shorten repairing time of the pulmonary artery, and feasibly can be applied in uniportal thoracoscopic lobectomy.


Assuntos
Hemostasia Cirúrgica/métodos , Neoplasias Pulmonares , Pneumonectomia/métodos , Artéria Pulmonar , Perda Sanguínea Cirúrgica/prevenção & controle , Constrição , Conversão para Cirurgia Aberta , Feminino , Técnicas Hemostáticas , Humanos , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Artéria Pulmonar/cirurgia , Estudos Retrospectivos , Cirurgia Torácica Vídeoassistida
18.
Zhongguo Gu Shang ; 33(7): 596-601, 2020 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-32700480

RESUMO

OBJECTIVE: To explore clinical effect of internal and external fixation combined with second-stage perforator fiap for the treatment of ankle fracture dislocation of Gustilo-Anderson types ⅢB and ⅢC. METHODS: From May 2014 to July 2017, 20 patients with Gustilo-Anderson types ⅢB and ⅢC ankle fracture dislocation were treated with internal and external fixation combined with second-stage perforator fiap, including 14 males and 6 females, aged from 18 to 58 years old with an average of (39.0±9.7) years old;17 patients were type ⅢB and 3 patients were type ⅢC according to Gustilo-Anderson classification;4 patients were type A, 7 patients were type B, and 9 patients were type C according to AO classification. The size of wound ranged from 4 cm×3 cm to 20 cm×9 cm. Second-stage perforator flap, 11 patients were performed with posterior tibial artery perforator flap, 5 patients were performed with fibular artery perforator flap, 1 patient was performed with anterior ankle flap, and 3 patients were performed with posterior tibial artery perforator flap combined with fibular artery perforator flap. Postoperative wound healing, flap survival and fracture healing were observed, AOFAS score was used to evaluate at the latest follow up. RESULTS: All limbs were preserved successfully without amputation. Nine patients occurred superficial infection without deep infection and osteomyelitis occurring. The flaps of 19 patients survived. All patients were followed up for 6 to 18 months with an average of (12.0±2.9) months. The flaps healed well without sinus tract, bone exposure and bone disunion occurring. Fracture healing time ranged from 4 to 10 months with an average of (6.6±1.7) months. PostoperativeAOFAS score was 76.7± 16.4, among which 4 patients got excellent result, 11 patients good, 3 patients fair, and 2 poor. CONCLUSION: Internal and external fixation combined with second stage perforator fiap for the treatment of ankle fracture dislocation of Gustilo-Anderson types ⅢB and ⅢC could effectively close the wound, improve fracture healing and restore appearance and function of limbs to the maximum.


Assuntos
Fratura-Luxação , Retalho Perfurante , Procedimentos Cirúrgicos Reconstrutivos , Lesões dos Tecidos Moles/cirurgia , Adolescente , Adulto , Tornozelo , Feminino , Fixação Interna de Fraturas , Humanos , Masculino , Pessoa de Meia-Idade , Transplante de Pele , Resultado do Tratamento , Adulto Jovem
19.
Oncol Rep ; 44(3): 1224-1232, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32705214

RESUMO

Ovarian cancer is one of the most lethal cancer types in American women. The platinum agent cisplatin, and/or paclitaxel, remains the first­line chemotherapy for ovarian cancer, but the treatment success is severely limited by chemoresistance. As previously reported, persistent STAT3 signaling is associated with resistance to cisplatin and paclitaxel. To investigate whether the STAT3 small molecule inhibitor LLL12 can enhance the treatment effect of cisplatin and paclitaxel in ovarian cancer cells, A2780, SKOV3, CAOV­3 and OVCAR5 cells were treated with LLL12, cisplatin and paclitaxel, alone or combination, and cell viability, cell migration, cell growth and protein expression levels were then evaluated. It was found that, for all four human ovarian cancer cell lines, STAT3 phosphorylation was significantly inhibited by LLL12. The combined treatment of LLL12 with paclitaxel or LLL12 with cisplatin exerted significantly greater inhibition of cell viability, cell migration and cell growth than did monotherapy. In addition, LLL12 and cisplatin in combination, or the three drugs in combination, also led to greater inhibition of cell viability and cell migration than combined cisplatin and paclitaxel treatment, a standard treatment for ovarian cancer. The present results demonstrated that the STAT3 small molecule inhibitor LLL12 is a potent inhibitor of STAT3 phosphorylation, cell viability and migration in human ovarian cancer cells. Combining LLL12 with cisplatin or paclitaxel may be a viable therapeutic approach in the treatment of patients with ovarian cancer exhibiting persistent STAT3 signaling.

20.
Medicine (Baltimore) ; 99(23): e20646, 2020 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-32502051

RESUMO

It is critical to accurately identify patients with severe acute pancreatitis (SAP) in a timely manner. This study aimed to develop a new simplified AP scoring system based on data from Chinese population.We retrospectively analyzed a consecutive series of 585 patients diagnosed with SAP at the Changhai hospital between 2009 and 2017. The new Chinese simple scoring system (CSSS) was derived using logistic regression analysis and was validated in comparison to 4 existing systems using receiver operating characteristic curves.Six variables were selected for incorporation into CSSS, including serum creatinine, blood glucose, lactate dehydrogenase, heart rate, C-reactive protein, and extent of pancreatic necrosis. The new CSSS yields a maximum total score of 9 points. The cut-offs for predicting mortality and severity (discriminating moderately SAP from SAP) were set as 6 points and 4 points respectively. Compared with 4 existing scoring systems, the area under the receiver operating characteristic of CSSS for prediction of mortality was 0.838, similar to acute physiology and chronic health evaluation II (0.844) and higher than Ranson's score (0.702, P < .001), bedside index of severity in acute pancreatitis (0.615), and modified computed tomography severity index (MCTSI) (0.736). For predicting SAP severity, CSSS was the most accurate (0.834), followed by acute physiology and chronic health evaluation II (0.800), Ranson's score (0.702), MCTSI (0.660), and bedside index of severity in acute pancreatitis (0.570). Further, the accuracy of predicting pancreatic infection with CSSS was the highest (0.634), similar to that of MCTSI (0.641).A new prognostic scoring system for SAP was derived and validated in a Chinese sample. This scoring system is a simple and accurate method for prediction of mortality.


Assuntos
Pancreatite Necrosante Aguda/mortalidade , Adulto , Idoso , Biomarcadores/sangue , Glicemia/metabolismo , Proteína C-Reativa/análise , China/epidemiologia , Creatinina/sangue , Feminino , Humanos , L-Lactato Desidrogenase/sangue , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Pancreatite Necrosante Aguda/sangue , Valor Preditivo dos Testes , Estudos Retrospectivos , Índice de Gravidade de Doença
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