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1.
Int J Biol Sci ; 16(11): 1861-1875, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32398955

RESUMO

Induced pluripotent stem cells (iPSCs), derived from reprogramming of somatic cells by a cocktail of transcription factors, have the capacity for unlimited self-renewal and the ability to differentiate into all of cell types present in the body. iPSCs may have therapeutic potential in regenerative medicine, replacing injured tissues or even whole organs. In this study, we examine epigenetic factors embedded in the specific 3-dimensional intrachromosomal architecture required for the activation of endogenous pluripotency genes. Using chromatin RNA in situ reverse transcription sequencing (CRIST-seq), we identified an Oct4-Sox2 binding long noncoding RNA, referred as to Osblr8, that is present in association with pluripotency status. Osblr8 was highly expressed in iPSCs and E14 embryonic stem cells, but it was silenced in fibroblasts. By using shRNA to knock down Osblr8, we found that this lncRNA was required for the maintenance of pluripotency. Overexpression of Osblr8 activated endogenous stem cell core factor genes. Mechanistically, Osblr8 participated in the formation of an intrachromosomal looping structure that is required to activate stem cell core factors during reprogramming. In summary, we have demonstrated that lncRNA Osblr8 is a chromatin architecture modulator of pluripotency-associated master gene promoters, highlighting its critical epigenetic role in reprogramming.

2.
Adv Mater ; : e2000380, 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-32329189

RESUMO

Boosting charge transfer in materials is critical for applications involving charge carriers. Engineering ionic channels in electrode materials can create a skeleton to manipulate their ion and electron behaviors with favorable parameters to promote their capacity and stability. Here, tailoring of the atomic structure in layered potassium niobate (K4 Nb6 O17 ) nanosheets and facilitating their application in lithium and potassium storage by dehydration-triggered lattice rearrangement is reported. The spectroscopy results reveal that the interatomic distances of the NbO coordination in the engineered K4 Nb6 O17 are slightly elongated with increased degrees of disorder. Specifically, the engineered K4 Nb6 O17 shows enhanced electrical and ionic conductivity, which can be attributed to the enlarged interlamellar spacing and subtle distortions in the fine atomic arrangements. Moreover, subsequent experimental results and calculations demonstrate that the energy barrier for Li+ /K+ diffusion is significantly lower than that in pristine K4 Nb6 O17 . Interestingly, the diffusion coefficient of K+ is one order of magnitude higher than that of Li+ , and the engineered K4 Nb6 O17 presents superior electrochemical performance for K+ to Li+ . This work offers an ionic engineering strategy to enable fast and durable charge transfer in materials, holding great promise for providing guidance for the material design of related energy storage systems.

3.
Nucleic Acids Res ; 48(7): 3935-3948, 2020 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-32055844

RESUMO

Formation of a pluripotency-specific chromatin network is a critical event in reprogramming somatic cells into pluripotent status. To characterize the regulatory components in this process, we used 'chromatin RNA in situ reverse transcription sequencing' (CRIST-seq) to profile RNA components that interact with the pluripotency master gene Oct4. Using this approach, we identified a novel nuclear lncRNA Oplr16 that was closely involved in the initiation of reprogramming. Oplr16 not only interacted with the Oct4 promoter and regulated its activity, but it was also specifically activated during reprogramming to pluripotency. Active expression of Oplr16 was required for optimal maintenance of pluripotency in embryonic stem cells. Oplr16 was also able to enhance reprogramming of fibroblasts into pluripotent cells. RNA reverse transcription-associated trap sequencing (RAT-seq) indicated that Oplr16 interacted with multiple target genes related to stem cell self-renewal. Of note, Oplr16 utilized its 3'-fragment to recruit the chromatin factor SMC1 to orchestrate pluripotency-specific intrachromosomal looping. After binding to the Oct4 promoter, Oplr16 recruited TET2 to induce DNA demethylation and activate Oct4 in fibroblasts, leading to enhanced reprogramming. These data suggest that Oplr16 may act as a pivotal chromatin factor to control stem cell fate by modulating chromatin architecture and DNA demethylation.

4.
Theranostics ; 10(1): 353-370, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31903125

RESUMO

Background: Long non-coding RNAs (lncRNAs) constitute an important component of the regulatory apparatus that controls stem cell pluripotency. However, the specific mechanisms utilized by these lncRNAs in the control of pluripotency are not fully characterized. Methods: We utilized a RNA reverse transcription-associated trap sequencing (RAT-seq) approach to profile the mouse genome-wide interaction targets for lncRNAs that are screened by RNA-seq. Results: We identified Peblr20 (Pou5F1 enhancer binding lncRNA 20) as a novel lncRNA that is associated with stem cell reprogramming. Peblr20 was differentially transcribed in fibroblasts compared to induced pluripotent stem cells (iPSCs). Notably, we found that Peblr20 utilized a trans mechanism to interact with the regulatory elements of multiple stemness genes. Using gain- and loss-of-function experiments, we showed that knockdown of Peblr20 caused iPSCs to exit from pluripotency, while overexpression of Peblr20 activated endogenous Pou5F1 expression. We further showed that Peblr20 promoted pluripotent reprogramming. Mechanistically, we demonstrated that Peblr20 activated endogenous Pou5F1 by binding to the Pou5F1 enhancer in trans, recruiting TET2 demethylase and activating the enhancer-transcribed RNAs. Conclusions: Our data reveal a novel epigenetic mechanism by which a lncRNA controls the fate of stem cells by trans-regulating the Pou5F1 enhancer RNA pathway. We demonstrate the potential for leveraging lncRNA biology to enhance the generation of stem cells for regenerative medicine.

5.
Proc Natl Acad Sci U S A ; 117(6): 2815-2823, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-31996477

RESUMO

Existing lithium-ion battery technology is struggling to meet our increasing requirements for high energy density, long lifetime, and low-cost energy storage. Here, a hybrid electrode design is developed by a straightforward reengineering of commercial electrode materials, which has revolutionized the "rocking chair" mechanism by unlocking the role of anions in the electrolyte. Our proof-of-concept hybrid LiFePO4 (LFP)/graphite electrode works with a staged deintercalation/intercalation mechanism of Li+ cations and PF6 - anions in a broadened voltage range, which was thoroughly studied by ex situ X-ray diffraction, ex situ Raman spectroscopy, and operando neutron powder diffraction. Introducing graphite into the hybrid electrode accelerates its conductivity, facilitating the rapid extraction/insertion of Li+ from/into the LFP phase in 2.5 to 4.0 V. This charge/discharge process, in turn, triggers the in situ formation of the cathode/electrolyte interphase (CEI) layer, reinforcing the structural integrity of the whole electrode at high voltage. Consequently, this hybrid LFP/graphite-20% electrode displays a high capacity and long-term cycling stability over 3,500 cycles at 10 C, superior to LFP and graphite cathodes. Importantly, the broadened voltage range and high capacity of the hybrid electrode enhance its energy density, which is leveraged further in a full-cell configuration.

6.
Medicine (Baltimore) ; 98(40): e17308, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31577723

RESUMO

Retroperitoneal laparoscopic pyeloplasty (RLP) is 1 method for treating ureteropelvic junction obstruction (UPJO) in children, but reports are more common in children than in infants younger than 2 years old. The purpose of this study was to evaluate the clinical value of RLP for infants with UPJO.From January 2015 to December 2017, a retrospective analysis of 22 infants aged 2 to 24 (11.95 ±â€Š6.00) months with UPJO who were treated with RLP in our hospital was performed. During the same period, 14 infants who underwent conventional transperitoneal laparoscopic pyeloplasty (TLP) were compared with those who underwent RLP. Postoperative recovery and complications, including bleeding, infection, urinary leakage and anastomotic stenosis, postoperative resumption of oral feeding, postoperative hospitalization time and surgical success rate were evaluated. Drainage and function were assessed with isotope scan at 6 months and later during the yearly follow-up and by intravenous urography (IVU) and mercaptoacetyltriglycine (MAG3) renography.Both groups underwent successful surgery. The operative time in the RLP group was 88 to 205 (120.59 ±â€Š24.59) min, and there was no significant difference compared with the TLP group (P = .767). The estimated intraoperative blood loss was 2 to 10 (3.75 ±â€Š1.59) ml, which was not significantly different between the 2 groups (P = .386). In the RLP group, the mean postoperative resumption of oral feeding was faster than that in the TLP group (3.55 ±â€Š0.74 vs 5.50 ±â€Š0.85 hour, P < .001), and the postoperative hospitalization time was shorter in the TLP group than in the RLP group (6.59 ±â€Š0.50 vs 7.07 ±â€Š0.47 day, P = .007 < .05). Follow-up lasted from 6 months to 3 years, and there was a significant reduction in postoperative hydronephrosis in both groups (P < .05, respectively).RLP is a safe procedure for infants. This procedure is associated with relatively little trauma, a quick recovery and good cosmetic effects. RLP also has the advantages of relatively little interference with the abdominal cavity and sufficient operating space; thus, this technique is worth promoting.


Assuntos
Pelve Renal/cirurgia , Laparoscopia/métodos , Espaço Retroperitoneal/cirurgia , Obstrução Ureteral/cirurgia , Perda Sanguínea Cirúrgica , Pré-Escolar , Feminino , Humanos , Hidronefrose/etiologia , Lactente , Tempo de Internação , Masculino , Nefrotomia , Duração da Cirurgia , Complicações Pós-Operatórias/epidemiologia , Estudos Retrospectivos , Obstrução Ureteral/complicações
7.
Mikrochim Acta ; 186(11): 706, 2019 10 21.
Artigo em Inglês | MEDLINE | ID: mdl-31637521

RESUMO

A glassy carbon electrode (GCE) was coated with N-doped carbon-modified palygorskite and used as an electrochemical sensor for determination of Pb(II) by differential pulse anodic stripping voltammetry. To obtain high reproducibility and sensitivity, optimum experimental conditions for lead deposition are studied. Voltammetric responses of the modified GCE prepared with different ratios of carbon and palygorskite are examined under same conditions. Compared with a bare GCE, a N-doped carbon modified/GCE and a bismuth-modified GCE, N-doped carbon-modified palygorskite greatly improves the performance of GCE. Response is the best and the interfacial impedance is minimized if the fraction of carbon coating is 31%. This indicates that its performance is due to the synergies between palygorskite and N-doped carbon. Figures of merit for the modified GCE include (a) a preconcentration time of 180 s, (b) a detection limit of 0.42 µg·L-1 (2σ criterion), and (c) a linear response in the 4.0 µg·L-1 to 10.0 mg·L-1 Pb(II) concentration range. The method is successfully applied to the determination of Pb(II) in spiked tape water and gives recoveries between 97.1 and 104.3%. Graphical abstract Schematic representation of different adsorption sites of Pb(II) and the optimal carbon content. The wide detection range is attributed to the synergetic effect of N-doped carbon modified palygorskite.

8.
Genome Res ; 29(9): 1521-1532, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31315906

RESUMO

Long noncoding RNAs (lncRNAs) can regulate the activity of target genes by participating in the organization of chromatin architecture. We have devised a "chromatin-RNA in situ reverse transcription sequencing" (CRIST-seq) approach to profile the lncRNA interaction network in gene regulatory elements by combining the simplicity of RNA biotin labeling with the specificity of the CRISPR/Cas9 system. Using gene-specific gRNAs, we describe a pluripotency-specific lncRNA interacting network in the promoters of Sox2 and Pou5f1, two critical stem cell factors that are required for the maintenance of pluripotency. The promoter-interacting lncRNAs were specifically activated during reprogramming into pluripotency. Knockdown of these lncRNAs caused the stem cells to exit from pluripotency. In contrast, overexpression of the pluripotency-associated lncRNA activated the promoters of core stem cell factor genes and enhanced fibroblast reprogramming into pluripotency. These CRIST-seq data suggest that the Sox2 and Pou5f1 promoters are organized within a unique lncRNA interaction network that determines the fate of pluripotency during reprogramming. This CRIST approach may be broadly used to map lncRNA interaction networks at target loci across the genome.


Assuntos
Cromatina/genética , Fator 3 de Transcrição de Octâmero/genética , RNA Longo não Codificante/genética , Fatores de Transcrição SOXB1/genética , Análise de Sequência de RNA/métodos , Animais , Sistemas CRISPR-Cas , Linhagem Celular , Reprogramação Celular , Fibroblastos/citologia , Fibroblastos/metabolismo , Camundongos , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/metabolismo , Regiões Promotoras Genéticas , Sequências Reguladoras de Ácido Nucleico
9.
ACS Nano ; 13(7): 7939-7948, 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31241893

RESUMO

Due to the abundant potassium resource on the Earth's crust, researchers now have become interested in exploring high-performance potassium-ion batteries (KIBs). However, the large size of K+ would hinder the diffusion of K ions into electrode materials, thus leading to poor energy/power density and cycling performance during the depotassiation/potassiation process. So, few-layered V5S8 nanosheets wrapping a hollow carbon sphere fabricated via a facile hollow carbon template induced method could reversibly accommodate K storage and maintain the structure stability. Hence, the as-obtained V5S8@C electrode enables rapid and reversible storage of K+ with a high specific capacity of 645 mAh/g at 50 mA/g, a high rate capability, and long cycling stability, with 360 and 190 mAh/g achieved after 500 and 1000 cycles at 500 and 2000 mA/g, respectively. The excellent electrochemical performance is superior to the most existing electrode materials. The DFT calculations reveal that V5S8 nanosheets have high electrical conductivity and low energy barriers for K+ intercalation. Furthermore, the reaction mechanism of the V5S8@C electrode in KIBs is probed via the in operando synchrotron X-ray diffraction technique, and it indicates that the V5S8@C electrode undergoes a sequential intercalation (KV5S8) and conversion reactions (K2S3) reversibly during the potassiation process.

10.
ACS Appl Mater Interfaces ; 11(5): 5373-5379, 2019 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-30632744

RESUMO

An ingeniously designed porous structure can synergistically optimize the desired properties and maximize the advantages of a material as an electrode for a high-performance energy storage system. The active material with a porous nanostructure could reduce the ion diffusion path and buffer the strain caused by the volume changes during cycling. Furthermore, combining the active material with a three-dimensional (3D) graphene aerogel (GA) matrix is an ideal way to maintain the structural integrity, improve the conductivity, and overcome the aggregation problem of the nanomaterials. Herein, we adopted a facile template-based strategy to derive a composite of 3D hierarchically porous cobalt phosphide nanocubes with a graphene aerogel (CoP@GA). The as-prepared CoP@GA features porous cobalt phosphide nanocubes that are firmly encapsulated and uniformly distributed in the well-defined graphene aerogel skeleton. Benefiting from the hierarchical porosity, structural integrity, and conductive network, the CoP@GA electrode manifests an ultrahigh initial Coulombic efficiency (88.6%), outstanding lithium storage performance in terms of excellent cycling performance (805.3 mAh·g-1 after 200 cycles at 200 mA·g-1), superior high-energy performance (351.8 mAh·g-1 after 4000 cycles at 10 A·g-1), and exceptional rate capability. Moreover, this synthesis protocol could be an instructive precedent for fabricating transition-metal-phosphide-based 3D porous composites with excellent electrochemical performances.

11.
Genome Biol ; 19(1): 218, 2018 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-30537986

RESUMO

BACKGROUND: Friend leukemia virus integration 1 (FLI1), an ETS transcription factor family member, acts as an oncogenic driver in hematological malignancies and promotes tumor growth in solid tumors. However, little is known about the mechanisms underlying the activation of this proto-oncogene in tumors. RESULTS: Immunohistochemical staining showed that FLI1 is aberrantly overexpressed in advanced stage and metastatic breast cancers. Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. Notably, FECR1 utilizes a positive feedback mechanism to activate FLI1 by inducing DNA hypomethylation in CpG islands of the promoter. FECR1 binds to the FLI1 promoter in cis and recruits TET1, a demethylase that is actively involved in DNA demethylation. FECR1 also binds to and downregulates in trans DNMT1, a methyltransferase that is essential for the maintenance of DNA methylation. CONCLUSIONS: These data suggest that FECR1 circular RNA acts as an upstream regulator to control breast cancer tumor growth by coordinating the regulation of DNA methylating and demethylating enzymes. Thus, FLI1 drives tumor metastasis not only through the canonical oncoprotein pathway, but also by using epigenetic mechanisms mediated by its exonic circular RNA.


Assuntos
Neoplasias da Mama/metabolismo , Regulação Neoplásica da Expressão Gênica , Proteína Proto-Oncogênica c-fli-1/metabolismo , Sistemas CRISPR-Cas , DNA (Citosina-5-)-Metiltransferase 1/metabolismo , Metilação de DNA , Humanos , Oxigenases de Função Mista/metabolismo , Metástase Neoplásica , Proteínas Proto-Oncogênicas/metabolismo
12.
Sci Data ; 5: 180255, 2018 11 20.
Artigo em Inglês | MEDLINE | ID: mdl-30457566

RESUMO

Pluripotent stem cells hold great investigative potential for developmental biology and regenerative medicine. Recent studies suggest that long noncoding RNAs (lncRNAs) may function as key regulators of the maintenance and the lineage differentiation of stem cells. However, the underlying mechanisms by which lncRNAs affect the reprogramming process of somatic cells into pluripotent cells remain largely unknown. Using fibroblasts and induced pluripotent stem cells (iPSCs) at different stages of reprogramming, we performed RNA transcriptome sequencing (RNA-Seq) to identify lncRNAs that are differentially-expressed in association with pluripotency. An RNA reverse transcription-associated trap sequencing (RAT-seq) approach was then utilized to generate a database to map the regulatory element network for lncRNA candidates. Integration of these datasets can facilitate the identification of functional lncRNAs that are associated with reprogramming. Identification of lncRNAs that regulate pluripotency may lead to new strategies for enhancing iPSC induction in regenerative medicine.


Assuntos
Reprogramação Celular/genética , Células-Tronco Pluripotentes , RNA Longo não Codificante , Transcriptoma , Diferenciação Celular/genética , Fibroblastos/citologia , Fibroblastos/metabolismo , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/metabolismo , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/metabolismo , RNA Longo não Codificante/análise , RNA Longo não Codificante/genética , Análise de Sequência de RNA
13.
Sci Data ; 5(1): 180255, 2018 11 20.
Artigo em Inglês | MEDLINE | ID: mdl-31745204

RESUMO

Pluripotent stem cells hold great investigative potential for developmental biology and regenerative medicine. Recent studies suggest that long noncoding RNAs (lncRNAs) may function as key regulators of the maintenance and the lineage differentiation of stem cells. However, the underlying mechanisms by which lncRNAs affect the reprogramming process of somatic cells into pluripotent cells remain largely unknown. Using fibroblasts and induced pluripotent stem cells (iPSCs) at different stages of reprogramming, we performed RNA transcriptome sequencing (RNA-Seq) to identify lncRNAs that are differentially-expressed in association with pluripotency. An RNA reverse transcription-associated trap sequencing (RAT-seq) approach was then utilized to generate a database to map the regulatory element network for lncRNA candidates. Integration of these datasets can facilitate the identification of functional lncRNAs that are associated with reprogramming. Identification of lncRNAs that regulate pluripotency may lead to new strategies for enhancing iPSC induction in regenerative medicine.

14.
Chempluschem ; 83(10): 915-923, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31950616

RESUMO

Ternary transition metal oxides (TTMOs) have attracted considerable attention for rechargeable batteries because of their fascinating properties. However, the unsatisfactory electrochemical performance originating from the poor intrinsic electronic conductivity and inferior structural stability impedes their practical applications. Here, the novel hierarchical porous NiO/ß-NiMoO4 heterostructure is fabricated, and exhibits high reversible capacity, superior rate capability, and excellent cycling stability in Li-ion batteries (LIBs), which is much better than the corresponding single-phase NiMoO4 and NiO materials. The significantly enhanced electrochemical properties can be attributed to its superior structural characteristics, including the large surface area, abundant pores, fast charge transfer, and catalytic effect of the intermediate product of metallic nickel. The NiO/ß-NiMoO4 heterostructure delivers a high capacity of 1314 mA h g-1 at 0.2 A g-1 after 100 cycles. Furthermore, even after 400 cycles at 1 A g-1 , the reversible capacity remains at around 500 mA h g-1 . These results indicate that the NiO/ß-NiMoO4 heterostructure shows great potential as an anode material for high-performance LIBs.

15.
Nanoscale ; 9(17): 5677-5685, 2017 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-28426060

RESUMO

Transition metal phosphide (TMP) nanostructures have stimulated increasing interest for use in water splitting owing to their abundant natural sources and high activity for the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER). Typically, the preparation of hierarchical TMPs involves the utilization of expensive or dangerous phosphorus sources, and, in particular, the understanding of topotactic transformations of the precursors to crystalline phases-which could be utilized to enhance electrocatalytic performance-remains very limited. We, herein, report a controllable preparation of CoP/CoP2 nanoparticles well dispersed in flower-like Al2O3 scaffolds (f-CoP/CoP2/Al2O3) as a bifunctional electrocatalyst for the HER and OER via the phosphorization of a flower-like CoAl layered double hydroxide precursor. Characterization by in situ X-ray diffraction (XRD) monitored the topotactic transformation underlying the controllable formation of CoP/CoP2via tuning the phosphorization time. Electrocatalytic tests showed that an f-CoP/CoP2/Al2O3 electrode exhibited a lower onset potential and higher electrocatalytic activity for the HER and OER in the same alkaline electrolyte than electrodes of flower-like and powdered CoP/Al2O3. The enhanced electrochemical performance was experimentally supported by measuring the electrochemically active surface area. The f-CoP/CoP2/Al2O3 composite further generated a current density of 10 mA cm-2 at 1.65 V when used as a bifunctional catalyst for overall water splitting. Our results demonstrate that the preparation route based on the LDH precursor may provide an alternative for investigating diverse TMPs as bifunctional electrocatalysts for water splitting.

16.
Antiviral Res ; 141: 165-173, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28263801

RESUMO

Coxsackie virus cannot be completely eliminated due to restrictive replication and impaired immune response, thus causing persistent infection. IL-10 plays a decisive role in the course of persistent viral infection. Umifenovir is a broad-spectrum antiviral drug, with certain treatment effects on Coxsackie virus infection. Previously, we showed that in addition to inhibiting Coxsackie B4 (CVB4) infection, Umifenovir also down-regulates IL-10 induced by persistent CVB4 virus infection in vitro and in vivo. Here, BALB/c mouse spleen cells infected with CVB4 were used as a model to explore the mechanism by which Umifenovir affects IL-10 expression. We found that subcellular localization of p38 and MAPK-activated protein kinase 2 (MK2) played a very important role in IL-10 secretion, and Umifenovir significantly prevented p38-MK2 complex from exiting the cell nucleus. This in turn blocked the biological functions of the latter pathway, and inhibited the high expression of IL-10 induced by CVB4. These findings suggest that Umifenovir is a potential anti-CVB4 drug; most importantly, Umifenovir could be used to treat IL-10 induced persistent viral infection.


Assuntos
Antivirais/farmacologia , Infecções por Coxsackievirus/tratamento farmacológico , Enterovirus Humano B/efeitos dos fármacos , Indóis/farmacologia , Interleucina-10/metabolismo , Animais , Antivirais/uso terapêutico , Infecções por Coxsackievirus/virologia , Modelos Animais de Doenças , Células HeLa , Humanos , Imunomodulação , Indóis/uso terapêutico , Interleucina-10/sangue , Interleucina-10/genética , Peptídeos e Proteínas de Sinalização Intracelular/química , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Camundongos , Miocardite/tratamento farmacológico , Miocardite/virologia , Proteínas Serina-Treonina Quinases/química , Proteínas Serina-Treonina Quinases/metabolismo , Baço/citologia , Baço/efeitos dos fármacos , Baço/virologia , Proteínas Quinases p38 Ativadas por Mitógeno/química , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
17.
Dalton Trans ; 46(3): 899-906, 2017 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-28004045

RESUMO

New phosphorescent, liquid crystalline cyclometalated tetradentate platinum complexes (Pt-L16, Pt-L12 and Pt-L6) based on the tetradentate C*N^N*C ligands (C*N^N*C = 6,6'-bis(4-(alkoxy)-phenoxy)-2,2'-bipyridine) are designed and synthesized. Their crystal structure, and photophysical, electrochemical and liquid crystal characteristics were investigated. The X-ray structure of Pt-L12 shows a severe distortion of this complex towards a tetrahedral geometry. All complexes are emissive both in degassed solution and in the solid state at room temperature with emission maxima in the red region of the spectrum. Pt-L16 and Pt-L12 show monotropic smectic liquid crystal characteristics. Moreover, these liquid crystal complexes can be aligned on a rubbed nylon-6 glass substrate and produce polarized emission with a dichroic ratio of 5.1.

18.
PLoS One ; 11(1): e0146597, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26751955

RESUMO

BACKGROUND: Although the application of ureteroscopy in the treatment of ureteral calculi during pregnancy has been on the rise, for persistent renal colic patients without ultrasound-detected ureteral calculi, it may represent a clinical dilemma due to the potential risks for both mother and fetus. OBJECTIVE: The aim of the present study is to present our experience with the application of the ureteroscope in the emergency treatment of persistent renal colic patients during pregnancy. METHODS: From March 2009 to September 2014, a total of 117 pregnant women who received ureteroscopy for persistent renal colic were retrospectively analyzed. Patients were divided into three groups according to duration of the persistent renal colic: Group A (within 12 hours; 24 cases); Group B (12 to 24 hours; 76 cases); and Group C (more than 24 hours; 17 cases). The stone-free rate, complications, and other qualitative data were analyzed. RESULTS: Of the 117 patients, 31 patients who were found not to have renal or ureteral calculi received ureteroscopic double-J (DJ) stent insertion, whereas 86 patients who were found with ureteral calculi received ureteroscopic lithotripsy (URSL) and DJ stent insertion. Among them, 24 patients (27.9%) were found with ureteral calculi by ureteroscopy rather than ultrasound. In addition, 73 patients (84.9%) had complete fragmentation of calculi; 12 patients (10.3%) had a threatened abortion (the rates of threatened abortion in Groups A, B and C were 8.3% vs. 6.5% vs. 29.4%; Group C compared with Groups A and B, p<0.05), and one patient (1.2%) had urosepsis (in Group C). However, these complications were cured with conservative treatment, without postpartum infant and maternal complications. CONCLUSION: For pregnant patients with persistent renal colic/ureteral calculi and hydronephrosis, ureteroscopic DJ stent insertion and URSL are effective and safe options when conservative treatment fails, even if no urinary calculi were found by ultrasound. At the same time, for patients with persistent renal colic during pregnancy, early application of ureteroscopy may reduce the risk of preterm birth.


Assuntos
Tratamento de Emergência , Cólica Renal/cirurgia , Ureteroscópios , Adolescente , Adulto , Feminino , Humanos , Gravidez , Adulto Jovem
19.
J Colloid Interface Sci ; 462: 183-90, 2016 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-26454377

RESUMO

Low-content ultrathin coating of non-active alumina (Al2O3) has been extensively utilized as one of the most effective strategies to improve electrochemical performances of electrodes for lithium-ion batteries (LIBs), however, typically by employing expensive atomic layer deposition equipment. We herein demonstrate a simple preparation of high-content and well-dispersed Al2O3 (24.33wt.%)-containing multi-component composite (CoO/Co3O4/N-C/Al2O3) by calcination of melamine/CoAl-layered double hydroxide (CoAl-LDH) mixture. The resulting composite bundles the advantages expected to improve electrochemical performances: (i) bi-active CoO/Co3O4, (ii) highly conductive N-doped carbon, and (iii) N-doped carbon and high-content non-active Al2O3 as buffering reagents, as well as (iv) good distribution of bi- and non-active components resulted from the lattice orientation and confinement effect of the LDH layers. Electrochemical evaluation shows that the composite electrode delivers a highly enhanced reversible capacity of 1078mAhg(-1) after 50cycles at 100mAg(-1), compared with the bi-active CoO/Co3O4 mixtures with and without non-active Al2O3. Transmission electron microscopy/scanning electron microscopy observations and electrochemical impedance spectra experimentally provide the information on the good distributions of multiple components and the improved conductivity underlying the enhancements, respectively. Our LDH precursor-based preparation route may be extended to design and prepare various multi-component transition metal oxides for efficient lithium storage.

20.
Oncotarget ; 6(27): 23764-75, 2015 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-26156017

RESUMO

Breast cancer is the most prevalent malignant disease in women worldwide. In patients with breast cancer, metastasis to distant sites directly determines the survival outcome. However, the molecular mechanism underlying metastasis in breast cancer remains to be defined. In this report, we found that Friend leukemia virus integration 1 (FLI1) proto-oncogene was differentially expressed between the aggressive MDA-MB231 and the non-aggressive MCF-7 breast cancer cells. Congruently, immunohistochemical staining of clinical samples revealed that FLI1 was overexpressed in breast cancers as compared with the adjacent tissues. The abundance of FLI1 protein was strongly correlated with the advanced stage, poor differentiation, and lymph node metastasis in breast cancer patients. Knockdown of FLI1 with small interfering RNAs significantly attenuated the potential of migration and invasion in highly metastatic human breast cancer cells. FLI1 oncoprotein activated the Rho GTPase pathway that is known to play a role in tumor metastasis. This study for the first time identifies FLI1 as a clinically and functionally important target gene of metastasis, providing a rationale for developing FLI1 inhibitors in the treatment of breast cancer.


Assuntos
Neoplasias da Mama/patologia , Invasividade Neoplásica/patologia , Metástase Neoplásica/patologia , Proteína Proto-Oncogênica c-fli-1/metabolismo , Proteínas rho de Ligação ao GTP/metabolismo , Neoplasias da Mama/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Ativação Enzimática , Feminino , Pontos de Checagem da Fase G1 do Ciclo Celular/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Células MCF-7 , Invasividade Neoplásica/genética , Metástase Neoplásica/genética , Proteína Proto-Oncogênica c-fli-1/genética , Interferência de RNA , RNA Interferente Pequeno/genética , Regulação para Cima
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