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1.
Genes (Basel) ; 13(9)2022 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-36140675

RESUMO

Saline soil is a worldwide distributed resource that seriously harms plants' growth and development. NaCl is the most widely distributed salt in saline soil. As a typical representative of halophytes, Tamarix ramosissima Lcdcb (T. ramosissima) is commonly grown in salinized soil, and halophytes have different abilities to retain more K+ under salt stress conditions. Halophytes can adapt to different salt environments by improving the scavenging activity of reactive oxygen species (ROS) by absorbing and transporting potassium (K+). In this study, electron microscope observation, hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents determination, primary antioxidant enzyme activity determination and transcriptome sequencing analysis were carried out on the leaves of T. ramosissima under NaCl stress at 0 h, 48 h and 168 h. The results showed that H2O2 and MDA contents increased in the 200 mM NaCl + 10 mM KCl and 200 mM NaCl groups, but the content increased the most in the 200 mM NaCl group at 168 h. In addition, the leaves of T. ramosissima in the 200 mM NaCl + 10 mM KCl group had the most salt secretion, and its superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities were all higher than those of the 200 mM NaCl group and significantly higher than those of the control group. According to the results of transcriptome sequencing, it was found that the expression of 39 genes related to antioxidant enzyme activity changed significantly at the transcriptional level. Among them, 15 genes related to antioxidant enzyme activities were upregulated, and 24 genes related to antioxidant enzyme activities were downregulated in the leaves of T. ramosissima when exogenous potassium (K+) was applied under NaCl stress for 48 h; when exogenous potassium (K+) was used for 168 h under NaCl stress, 21 antioxidant enzyme activity-related genes were upregulated, and 18 antioxidant enzyme activity-related genes were downregulated in T. ramosissima leaves. Based on the changes of expression levels at different treatment times, 10 key candidates differentially expressed genes (DEGs) (Unigene0050462, Unigene0014843, Unigene0046159, Unigene0046160, Unigene0008032, Unigene0048033, Unigene0004890, Unigene0015109, Unigene0020552 and Unigene0048538) for antioxidant enzyme activities were further screened. They played an important role in applying exogenous potassium (K+) for 48 h and 168 h to the leaves of T. ramosissima in response to NaCl stress. Their expression levels were dominated by upregulation, which enhanced the activity of antioxidant enzymes, and helped T. ramosissima mitigate NaCl poison and resist NaCl stress. Particularly, Unigene0048538 in glutathione S-transferase (GST) activity had the largest log2 fold-change in the comparison groups of 200 mM NaCl-48 h vs. 200 mM NaCl + 10 mM KCl-48 h and 200 mM NaCl-168 h vs. 200 mM NaCl + 10 mM KCl-168 h. Its expression level was upregulated and played an important role in NaCl toxicity. At the same time, the results of the phylogenetic tree analysis showed that Unigene0048538 had the closest genetic distance to Prunus persica in the evolutionary relationship. In summary, with the increase of exogenous potassium (K+) application time under NaCl stress, T. ramosissima can resist high NaCl stress by enhancing antioxidant enzymes' activity and maintaining the growth of T. ramosissima. Still, it is not enough to completely eliminate NaCl poison. This study provides a theoretical basis for the molecular mechanism of salt tolerance and K+ mitigation of NaCl poison by the representative halophyte T. ramosissima in response to NaCl stress.

2.
Nanomaterials (Basel) ; 12(18)2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36144881

RESUMO

Due to its unique physical and chemical properties, MXene has recently attracted much attention as a promising candidate for wastewater treatment. However, the low water permeation flux of MXene membrane remains a challenge that has not been fully solved. In this study, attapulgite was used to increase the flux of MXene membrane through a facile one-pot method, during which the MXene nanosheets were self-assembled while being intercalated by the attapulgite nanorods to finally form the composite membranes. Under optimal conditions, an increase of water permeation flux of 97.31% could be observed, which was attributed to the broadened nano-channel upon the adequate intercalation of attapulgite nanorods. Its permeation flux and rejection rate for methylene blue (MB) were further studied for diverse applications. In contrast to bare MXene, the permeation flux increased by 61.72% with a still high rejection rate of 90.67%, owing to the size rejection. Overcoming a key technique barrier, this work successfully improved the water permeability of MXene by inserting attapulgite nanorods, heralding the exciting prospects of MXene-based lamellar membrane in dye wastewater treatment.

3.
Eur J Epidemiol ; 2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-36031647

RESUMO

Pyrethroid insecticides have been extensively used worldwide, but few studies explored the prospective association between pyrethroid exposure and incident type 2 diabetes (T2D). We conducted a nested case-control study of 2012 paired cases and controls, and measured eight pyrethroid insecticides in the baseline sera. We used conditional logistic regression models to estimate odds ratios (ORs) with 95% confidence intervals, and constructed multiple-pollutant models to investigate the association of pyrethroid mixture with incident T2D risk. The median concentrations (detection rates) were 3.53 µg/L (92.45%), 0.52 µg/L (99.80%), 1.16 µg/L (90.61%) and 1.43 µg/L (99.95%) for permethrin, cypermethrin, fenvalerate, and deltamethrin, respectively. Compared to participants with serum fenvalerate levels in the first quartile, the multivariable-adjusted ORs of incident T2D were 1.20 (95% CI 0.86-1.67), 1.41 (0.97-2.05), and 2.29 (1.27-4.11) for the second, third and fourth quartile (P trend = 0.01). Spline analysis further confirmed the positive association between serum fenvalerate levels and incident T2D risk (P for overall association = 0.006). Furthermore, mixture models revealed a positive association of pyrethroid mixture with incident T2D risk, with serum fenvalerate ranked as the top contributor (proportion of relative contribution: > 70%). We found that high concentrations of serum pyrethroid insecticides were significantly associated with an increased risk of incident T2D. The elevated risk was largely explained by fenvalerate. Further investigations are urgently needed to confirm our findings and elucidate the underlying mechanisms, given the widespread use of pyrethroids and the global pandemic of diabetes.

4.
Bioresour Technol ; 361: 127757, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35952860

RESUMO

In this study, a bioelectrochemical system consisting of pyrite-based autotrophic denitrification (PAD) and heterotrophic denitrification (HD) was established to polish nitrate wastewater. The loading of electric current (EC) could stimulate the dissolution of pyrite. Appropriate EC (I ≤ 30 mA) was conducive to nitrate removal, too high EC (I = 40 mA) would inhibit nitrate removal and lead to an obvious accumulation of NO2--N and NH4+-N. Microbial analysis revealed that the increase of EC could inhibit the diversity of heterotrophic microbes, but appropriate EC (I = 10 mA) could increase the diversity of autotrophic microbes. The EC loading was conducive to the enrichment of iron autotrophic denitrifiers (Ferritrophicum), pyrite-oxidizing bacteria (Thiobacillus, Sulfurimonas), and sulfur autotrophic denitrifiers (Dechloromonas, Thiobacillus, and Arenimonas). The EC loading enlarged the contribution of PAD, making PAD a dominant pathway in denitrification.


Assuntos
Desnitrificação , Nitratos , Processos Autotróficos , Biofilmes , Reatores Biológicos/microbiologia , Eletrodos , Ferro , Nitratos/metabolismo , Nitrogênio/metabolismo , Poliésteres , Sulfetos
5.
Front Public Health ; 10: 900294, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35958856

RESUMO

Purpose: To explore the effect of human papillomavirus (HPV) status on prognosis and further investigate whether human papillomavirus (HPV) status has an impact on the local treatment strategies for T1-2N0 oropharyngeal squamous cell cancer (OPSCC) patients. Methods: Patients diagnosed with T1-2N0 OPSCC between 2010 and 2015 were included from the Surveillance, Epidemiology, and End Results database. Data were analyzed using propensity score matching (PSM), Chi-square test, Kaplan-Meier survival analysis, and Cox multivariable analyses. Results: A total of 1,004 patients were identified, of whom 595 (59.3%) had HPV-related tumors. Of all the patients, 386 (38.4%) and 618 (61.6%) received definitive radiotherapy and radical surgery, respectively. HPV status had no significant effect on local treatment strategies for early-stage OPSCC (P = 0.817). The 3-year cancer-specific survival (CSS) and overall survival (OS) were 89.6 and 80.1%, respectively. Compared to those with HPV-negative diseases, patients with HPV-positive diseases had better CSS and OS. A total of 222 pairs of patients were completely matched after PSM. The results of multivariate Cox regression analysis showed that patients with HPV-positive disease had significantly better CSS (P = 0.001) and OS (P < 0.001) compared to those with HPV-negative tumors. However, local treatment strategy was not associated with survival outcomes after PSM (CSS, P = 0.771; OS, P = 0.440). The subgroup analysis showed comparable CSS and OS between those treated with radical surgery and definitive radiotherapy regardless of HPV status. Conclusions: HPV status is an independent prognostic factor for the survival of stage T1-2N0 OPSCC patients. Local treatment strategies had no significant effect on the survival of early-stage OPSCC regardless of HPV status. Patients with early-stage OPSCC should be informed regarding the pros and cons of definitive radiotherapy or radical surgery.


Assuntos
Alphapapillomavirus , Carcinoma de Células Escamosas , Neoplasias de Cabeça e Pescoço , Neoplasias Orofaríngeas , Infecções por Papillomavirus , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/cirurgia , Células Epiteliais/patologia , Neoplasias de Cabeça e Pescoço/complicações , Humanos , Neoplasias Orofaríngeas/patologia , Neoplasias Orofaríngeas/cirurgia , Papillomaviridae , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/patologia , Prognóstico , Carcinoma de Células Escamosas de Cabeça e Pescoço/complicações
6.
Int J Mol Sci ; 23(16)2022 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-36012595

RESUMO

Soil salinization is one of the main environmental factors affecting plant growth worldwide. Tamarix ramosissima Ledeb. (T. ramosissima) is a halophyte representative that is widely grown in salinized soils. As an important nutrient element for plant growth, K+ plays an important role in improving the tolerance to salt stress, but the mechanism of reducing the damage caused by NaCl stress to T. ramosissima is less reported. Our results show that the proline content and the Log2 fold-change of proline's relative quantification in the roots of T. ramosissima increased over time with the application of exogenous potassium (K+) for 48 h and 168 h under NaCl stress. Moreover, 13 amino-acid-related metabolic pathways were involved in the resistance of T. ramosissima to salt stress. Mainly, the aldehyde dehydrogenase family genes and tryptophan-synthase-related genes were found at 48 h and 168 h with exogenous potassium applied to the roots of T. ramosissima under NaCl stress, and they regulated their related metabolic accumulation in the arginine and proline metabolism pathways, increasing the effectiveness of inducing NaCl tolerance of T. ramosissima. It is noteworthy that alpha-ketobutyric was produced in the roots of T. ramosissima under NaCl stress for 48 h with the application of exogenous potassium, which is one of the most effective mechanisms for inducing salt tolerance in plants. Meanwhile, we found three DEGs regulating alpha-ketobutyric acid. This study provides a scientific theoretical basis for further understanding the molecular mechanism of K+ alleviating the salinity damage to T. ramosissima caused by NaCl.


Assuntos
Tamaricaceae , Aminoácidos/metabolismo , Raízes de Plantas/metabolismo , Potássio/metabolismo , Prolina/metabolismo , Cloreto de Sódio/metabolismo , Cloreto de Sódio/farmacologia , Solo/química , Estresse Fisiológico
7.
Int J Biol Macromol ; 218: 1-8, 2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-35792312

RESUMO

Polycaprolactone (PCL) nanofibers have become an ideal material for bone tissue engineering due to a series of advantages. Considering the clinical treatment of bone defects, in addition to meeting the golden standard, PCL based nanofibers also need to be multifunctional to anti-inflammatory, antibacterial properties, and enhance the bone regeneration and repair. Herein, we successfully developed the multifunctional PCL/LIG/ZIF-8 composite nanofibers by loading ZIF-8 on electrospun PCL/lignin (PCL/LIG) nanofibers. The prepared composite nanofibers exhibit fairly good wettability and acceptable degradation rate, as well as excellent antioxidative stress and antibacterial properties originating from the incorporated LIG and loaded ZIF-8. Moreover, owing to the synergistic effect of LIG and ZIF-8, the composite nanofibers present excellent osteogenic differentiation, which can be verified in biomineralization experiments and real-time quantitative polymerase chain reaction. These results indicate that the PCL/LIG/ZIF-8 composite nanofibers, as potential healthcare candidate, have a promising applied in the treatment of bone defects.


Assuntos
Nanofibras , Antibacterianos/uso terapêutico , Lignina , Nanofibras/uso terapêutico , Osteogênese , Poliésteres , Engenharia Tecidual/métodos , Tecidos Suporte
8.
Genes (Basel) ; 13(8)2022 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-35893048

RESUMO

Potassium ion (K+) channels and transporters are key components of plant K+ absorption and transportation and play an important role in plant growth and development. This study revealed that K+ channels and transporters are involved in the salt tolerance molecular mechanism and metabolites of the halophyte representative plant Tamarix&nbsp;ramosissima (T. ramosissima) in response to NaCl stress, providing a theoretical basis for the mitigation of salt stress using halophytes. Through transcriptome sequencing and metabolite detection analysis of 0 h, 48 h and 168 h by applying exogenous K+ to the roots of T. ramosissima under NaCl stress, 15 high-quality Clean Data bases were obtained, Q20 reached more than 97%, Q30 reached more than 92%, and GC content reached 44.5%, which is in line with further bioinformatics analysis. Based on the Liquid chromatography-mass spectrometry (LC-MS) analysis, the roots of T. ramosissima were exposed to exogenous potassium for 48 h and 168 h under NaCl stress, and 1510 and 1124 metabolites were identified in positive and negative ion mode, respectively. Through orthogonal projections to latent structures discriminant analysis (OPLS-DA) model analysis, its metabolomic data have excellent predictability and stability. The results of this study showed that there were 37 differentially expressed genes (DEGs) annotated as Class 2 K+ channels (Shaker-like K+ channel and TPK channel) and Class 3 K+ transporters (HAK/KUP/KT, HKT and CPAs transporter families). Among them, 29 DEGs were annotated to the gene ontology (GO) database, and the most genes were involved in the GO Biological Process. In addition, the expression levels of Unigene0014342 in the HAK/KUP/KT transporter and Unigene0088276 and Unigene0103067 in the CPAs transporter both first decreased and then increased when treated with 200 mM NaCl for 48 h and 168 h. However, when treated with 200 mM NaCl + 10 mM KCl for 48 h and 168 h, a continuous upward trend was shown. Notably, the expression level of Unigene0016813 in CPAS transporter continued to increase when treated with 200 mM NaCl and 200 mM NaCl + 10 mM KCl for 48 h and 168 h. 3 DEGs, Unigene0088276, Unigene0016813 and Unigene0103067, were dominated by the positive regulation of their related metabolites, and this correlation was significant. The results showed that these DEGs increased the absorption of K+ and the ratio of K+/Na+ under NaCl stress at 48 h and 168 h after adding exogenous potassium and enhanced the salt tolerance of T. ramosissima. Notably, the expression level of Unigene0103067 in the CPAs transporter was consistently upregulated when 200 mM NaCl + 10 mM KCl was treated for 48 h and 168 h. The positive regulatory metabolites were always dominant, which better helped T. ramosissima resist salt stress. Unigene0103067 plays an important role in enhancing the salt tolerance of T. ramosissima and reducing the toxicity of NaCl in roots. Additionally, phylogenetic tree analysis showed that Unigene0103067 and Reaumuria&nbsp;trigyna had the closest genetic distance in the evolutionary relationship. Finally, 9 DEGs were randomly selected for quantitative real-time PCR (qRT-PCR) verification. Their expression trends were completely consistent with the transcriptome sequencing analysis results, proving that this study's data are accurate and reliable. This study provides resources for revealing the molecular mechanism of NaCl stress tolerance in T. ramosissima and lays a theoretical foundation for cultivating new salt-tolerant varieties.


Assuntos
Potássio , Tamaricaceae , Filogenia , Plantas/metabolismo , Potássio/metabolismo , Cloreto de Sódio/metabolismo , Tamaricaceae/genética , Tamaricaceae/metabolismo , Transcriptoma
9.
Environ Pollut ; 307: 119581, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35680067

RESUMO

Observational studies reported inconsistent results on the association between bisphenol A (BPA) and type 2 diabetes (T2D) risk. Whether genetic factors modified the association remains unclear. The present nested case-control study prospectively investigated the association of BPA with T2D risk, and the interaction and combined effects of diabetes genetic risk score (GRS) and serum BPA on T2D risk. Based on the Dongfeng-Tongji cohort study, 995 incident diabetes cases and 1:1 age- and gender-matched controls were included. T2D was diagnosed based on the American Diabetes Association criteria. Serum BPA concentration was measured at baseline. Diabetes GRS was constructed by 88 diabetes-related SNPs selected from large-scale GWASs. A U-shaped association was observed between serum BPA levels and T2D risk, with the lowest odds of T2D at the serum BPA levels of 1.00 ng/mL (P = 0.001 for nonlinearity). Compared with the middle group, the multivariate-adjusted ORs of T2D in the lowest group and the highest group of serum BPA were 1.52 (95% CI: 1.04, 2.22) and 1.40 (95% CI: 1.08, 1.81), respectively. Both serum BPA levels (ß = 0.107, P = 0.001) and weighted-GRS (w-GRS) (ß = 0.072, P = 0.02) were significantly associated with baseline FPG levels. Participants with both highest w-GRS and serum BPA levels had highest risk of T2D (OR = 2.53, 95%CI: 1.49, 4.31, P = 0.001) and higher baseline FPG levels (ß = 0.218, P = 0.01), compared with those with both lowest w-GRS and serum BPA levels. Non modified effects of serum BPA levels and w-GRS on T2D, baseline FPG levels, and 5-y changes of FPG levels were detected (All Pinteraction > 0.05). Our results suggested a U-shaped association between serum BPA levels and T2D risk. Participants with higher serum BPA levels and diabetes genetic risk had higher FPG levels and higher risk of T2D.


Assuntos
Compostos Benzidrílicos , Diabetes Mellitus Tipo 2 , Fenóis , Compostos Benzidrílicos/farmacologia , Estudos de Casos e Controles , Estudos de Coortes , Diabetes Mellitus Tipo 2/induzido quimicamente , Diabetes Mellitus Tipo 2/genética , Humanos , Fenóis/farmacologia , Fatores de Risco
10.
J Affect Disord ; 312: 198-207, 2022 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-35728679

RESUMO

BACKGROUND: Previous studies have reported that Internet addiction (IA) is an important predictor of aggression. However, little is known whether aggression is a risk factor of IA. This study aims to explore whether IA can predict total and sub-types of aggression, whether aggression also can predict IA, and the potential sex difference. METHOD: A total of 15,977 adolescents aged 12 to 18 were recruited though a stratified cluster sampling across five representative provinces in mainland of China. Young's Internet Addiction Test (IAT) and Buss and Warren's Aggression Questionnaire (BWAQ) were used to measure IA and aggression, respectively. RESULTS: The prevalence of IA and total aggression was 16.8 % and 25.0 %, respectively. Binary logistic regression analysis indicated that IAT score was significantly associated with total and five sub-types of aggression (P < 0.001). However, scores of physical aggression, hostility, and indirect aggression were positively associated with IA (P < 0.001), while verbal aggression and anger were not significant (P > 0.05). Moreover, the interaction of sex and IA was significant for aggression. Specifically, females with IA had higher risks of anger and indirect aggression but had lower risks of physical and verbal aggression compared to males with IA. LIMITATIONS: Cross-sectional design, self-reported information, and only Chinese rural adolescents. CONCLUSIONS: IA is a strong risk factor of total and five sub-types of aggression, while not all sub-types of aggression are predictors of IA. Prevention programs for adolescents' IA and aggression should consider different aggression sub-types and sex.


Assuntos
Comportamento Aditivo , Transtorno de Adição à Internet , Adolescente , Agressão , Comportamento Aditivo/epidemiologia , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Internet , Masculino , Caracteres Sexuais , Inquéritos e Questionários
11.
Int J Immunopathol Pharmacol ; 36: 3946320221104548, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35609253

RESUMO

OBJECTIVES: Glioma is a common type of brain tumor with high incidence and mortality rates. Procollagen C-protease enhancer protein (PCOLCE) has been shown to regulate tumor growth and metastasis in several cancers. However, the role of PCOLCE in glioma is unknown. This study aims to assess the association between PCOLCE and prognosis of glioma, and investigated the potential mechanisms. METHODS: The prognostic value of PCOLCE was determined using data from nine publicly available glioma cohorts. We also investigated the relationship between PCOLCE and glioma immune microenvironment and predicted response to immunotherapy based on the expression levels of PCOLCE. The potential roles of PCOLCE in glioma were also explored and validated in cell experiment. RESULTS: Survival analysis suggested that high-PCOLCE expression was associated with poor prognosis in glioma. Upregulation of PCOLCE enhanced an immune suppressive microenvironment in glioma by regulating immunocyte infiltration and Cancer-Immunity Cycle. Cox and ROC analysis revealed that PCOLCE was a prognostic factor for glioma and could be used to predict survival of the patients. Patients with low-PCOLCE expression were more likely to respond to Immunotherapy with ICI (immune checkpoint inhibitor) and survive longer. Enrichment analysis showed that PCOLCE was associated with multiple tumor-related pathways. Finally, we demonstrated that the knockdown of PCOLCE inhibited glioma development by regulating cell cycle and promoting apoptosis in in vitro experiments. CONCLUSION: PCOLCE promotes glioma progression by regulating multiple tumor-related pathways and immune microenvironment and can be used as a prognostic factor for glioma.


Assuntos
Neoplasias Encefálicas , Glioma , Biomarcadores Tumorais/genética , Humanos , Peptídeo Hidrolases , Pró-Colágeno/metabolismo , Prognóstico , Microambiente Tumoral
12.
Artigo em Inglês | MEDLINE | ID: mdl-35564999

RESUMO

Considerable research has shown that religion operates as a protective factor for one's health. However, there is still a lack of understanding of the mechanisms by which religion is linked to individual health and wellbeing, especially in predominantly secular societies. This study tried to address this gap by developing a theoretical model to examine how religiosity is related to life satisfaction and health perception in a non-Western culture. Macau, a Portuguese colony until 1999, remains a diversified culture because of its intermixed historical background from the East and the West. Through structural equation modeling, the analysis of data collected from a representative sample of Macau residents, using a multistage stratified sampling procedure, indicated a positive link between religiosity and health. Moreover, altruism and prejudice mediated a portion of the relationship between religiosity and health. Additionally, our results demonstrated that Macau residents who were more religious had a higher level of altruism and a lower level of prejudice. The link between religion and prejudice in Macau differs from that of many other cultures, indicating that the effect of religion on prejudice varies by cultural context. In sum, our study showed that even in the shadow of glittering casinos, religion is positively related to health.


Assuntos
Preconceito , Religião , Etnicidade , Humanos , Macau
13.
Biodegradation ; 33(4): 317-331, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35522400

RESUMO

Up to date, the intrinsic association of nitrate loading rate (NLR) with treatment performance of solid-phase denitrification (SPD) systems is still ambiguous. To address this issue, three continuous up-flow bioreactors were configured. They were packed with polycaprolactone (PCL) under a filling ratio of 30%, 60% or 90% and were operated under a varying NLR of 0.34 ± 0.01-3.99 ± 0.12 gN/(L·d). Results showed that the denitrification efficiency was high (RE > 96%) and stable except the case with the highest NLR, which was mainly attributed to the lack of available carbon sources. At the phylum or genus level, most of the detected dominant bacterial taxa were either associated with organics degradation or nitrogen metabolism. The difference in bacterial community structure among the three stages was mainly caused by NLR rather than the filling ratio. Moreover, as the NLR got higher, the Bray-Curtis distance between samples from the same stage became shorter. By the results of gene or enzyme prediction performed in PICRUSt2, the main nitrogen metabolism pathways in these reactors were denitrification, dissimilatory nitrate reduction to ammonium (DNRA), assimilatory nitrate reduction to ammonium (ANRA) and nitrogen fixation. Moreover, aerobic and anaerobic nitrate dissimilation coexisted in the systems with the latter playing a dominant role. Finally, denitrification and DNRA occurred under both high and low NLR conditions while nitrogen fixation and ANRA preferred to occur under low NLR environments. These findings might help guide practical applications.


Assuntos
Compostos de Amônio , Nitratos , Biodegradação Ambiental , Desnitrificação , Nitratos/análise , Nitrogênio/química , Oxirredução , Poliésteres
14.
Front Oncol ; 12: 845036, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35494053

RESUMO

Calcium/calmodulin-dependent protein ID (CAMK1D) is widely expressed in many tissues and involved in tumor cell growth. However, its role in gliomas has not yet been elucidated. This study aimed to investigate the roles of CAMK1D in the proliferation, migration, and invasion of glioma. Through online datasets, Western blot, and immunohistochemical analysis, glioma tissue has significantly lower CAMK1D expression levels than normal brain (NB) tissues, and CAMK1D expression was positively correlated with the WHO classification. Kaplan-Meier survival analysis shows that CAMK1D can be used as a potential prognostic indicator to predict the overall survival of glioma patients. In addition, colony formation assay, cell counting Kit-8, and xenograft experiment identified that knockdown of CAMK1D promotes the proliferation of glioma cells. Transwell and wound healing assays identified that knockdown of CAMK1D promoted the invasion and migration of glioma cells. In the above experiments, the results of overexpression of CAMK1D were all contrary to those of knockdown. In terms of mechanism, this study found that CAMK1D regulates the function of glioma cells by the PI3K/AKT/mTOR pathway. In conclusion, these findings suggest that CAMK1D serves as a prognostic predictor and a new target for developing therapeutics to treat glioma.

15.
Environ Res ; 212(Pt B): 113293, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-35427595

RESUMO

Evidence on the associations of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) with hypertension or blood pressure (BP) levels was limited and inconsistent. The present prospective study aims to evaluate the longitudinal associations of serum levels of PFOA and PFOS with incident hypertension risk and change of blood pressure levels. At baseline 1080 participants (mean age 62 years, 58.9% females) free of hypertension, cardiovascular disease, diabetes, and cancer were followed up for nearly 5 years. Baseline serum levels of PFOA and PFOS were measured with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS). Hypertension was defined as any of (1) self-reported physician-diagnosed hypertension (2) use of hypotension drugs (3) measured systolic BP ≥ 140 mmHg or diastolic BP ≥ 90 mmHg. Change of BP was evaluated as a difference between twice measurements (BP at follow-up visit-BP at baseline). After adjustment for multiple covariates, serum PFOS levels were negatively correlated with risk of hypertension [RR per lg-unit = 0.94 (95% CI: 0.88, 0.99)] and change of systolic BP [ß = -1.48 (95% CI: -2.56, -0.41)]. The highest vs lowest quartiles of PFOS concentration was negatively associated with hypertension risk. Compared with Q1, the RRs (95% CIs) for Q2, Q3, and Q4 were 0.83 (0.67-0.98), 0.81 (0.67-0.97), and 0.81(0.67-0.97), respectively (p for trend = 0.016). The negative associations remained in females but not in males (p for interaction = 0.44). No significant association of PFOA with hypertension risk was observed. Further studies are needed to validate our findings.


Assuntos
Ácidos Alcanossulfônicos , Fluorcarbonetos , Hipertensão , Ácidos Alcanossulfônicos/toxicidade , Pressão Sanguínea , Caprilatos/toxicidade , Cromatografia Líquida , Feminino , Fluorcarbonetos/toxicidade , Humanos , Hipertensão/induzido quimicamente , Hipertensão/epidemiologia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Espectrometria de Massas em Tandem
16.
Small ; 18(14): e2106534, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35182023

RESUMO

Electrocatalytic reduction of oxygen (O2 ) to produce hydrogen peroxide (H2 O2 ) frequently suffers from the low activity and poor selectivity of catalysts owing to the lack of systematic strategies. The resulting enhancement to enable the further design of a new bimetallic catalyst with the synergistic interplay, as exemplified by Cu-Pb catalyst for two-electron oxygen reduction reaction (2e- ORR), is reported here. Critically, in-depth evidence, including density functional theory (DFT) calculations, electrochemical signals, in-situ Raman, and H2 O2 -proof work, allude to a catalytic favor to the 2e- ORR of Cu-Pb.


Assuntos
Chumbo , Oxigênio , Catálise , Peróxido de Hidrogênio
17.
J Gerontol B Psychol Sci Soc Sci ; 77(8): 1442-1453, 2022 08 11.
Artigo em Inglês | MEDLINE | ID: mdl-35092423

RESUMO

OBJECTIVES: Social ties increase in importance in late life and narcissism may be deleterious to these ties. More narcissistic older adults may have more frequent social encounters than less narcissistic people and may prefer weak ties (e.g., acquaintances) over close ones (e.g., family, close friends). They may benefit more from these encounters due to their need for adulation. This study examined how daily social experiences and mood varied by narcissism among older adults. METHODS: Older adults aged 65-92 years (N = 303) completed the Narcissistic Personality Inventory-16 and completed ecological momentary assessments in which they reported number, type, and quality of social contacts and positive and negative mood every 3 h for 5-6 days. RESULTS: In multilevel models, narcissism did not predict the number or pleasantness of social encounters. But more narcissistic older adults reported a greater percentage of stressful discussions with weak ties. With regard to mood, more narcissistic people reported higher negative mood if they had more encounters with weak ties and when discussing something stressful with weak ties. Less narcissistic people reported lowered positive mood after they discussed something stressful with close ties. DISCUSSION: The findings present a nuanced understanding of how the self-centeredness of narcissism may be manifest in late life. More narcissistic people may be less sensitive to close partner's (e.g., family, friends) distress, but their mood may be more susceptible to negative social events, especially with weak ties (e.g., acquaintances).


Assuntos
Narcisismo , Transtornos da Personalidade , Afeto , Idoso , Emoções , Amigos , Humanos
18.
Gerontologist ; 62(4): 519-529, 2022 04 20.
Artigo em Inglês | MEDLINE | ID: mdl-34240145

RESUMO

BACKGROUND AND OBJECTIVES: Environmental gerontology and environmental psychology theories address adaptations of living space for disability and individual preferences. This study combines these perspectives to examine how room décor (i.e., furnishings, design, decoration) corresponds with functional limitations and personality in late life. RESEARCH DESIGN AND METHODS: Older adults aged 65 and older (N = 286) completed interviews regarding living arrangements, functional limitations, personality, and depressive symptoms. Participants provided 3-4 photographs of the room where they spend the most time. Raters coded photographs for physical adaptations for functional limitations and 19 features of décor (e.g., crowding, color), fitting 3 categories: (a) newness, (b) comfort, and (c) cheerfulness. We estimated linear regression models to examine how functional limitations or personality are associated with room décor, and whether living arrangement moderates these links. We also assessed whether room décor moderates functional limitations or personality predicting depressive symptoms. RESULTS: Functional limitations were associated with greater clutter and less brightness. Extraversion was associated with newness and cheerfulness (but not comfort), and conscientiousness with newness and comfort (but not cheerfulness). Openness was associated with more newness and cheerfulness for those who live alone. Moderation models revealed functional limitations were associated with fewer depressive symptoms if the room was more cluttered. Conscientiousness was negatively associated with depressive symptoms when the room was higher on newness or comfort. DISCUSSION AND IMPLICATIONS: Findings generally supported environmental psychology and environmental gerontology perspectives and suggest "goodness of fit" between functional abilities, personal desires, and room characteristics may contribute to benefits of aging in place.


Assuntos
Pessoas com Deficiência , Vida Independente , Atividades Cotidianas/psicologia , Idoso , Humanos , Vida Independente/psicologia , Personalidade , Características de Residência
19.
Ecotoxicol Environ Saf ; 229: 113060, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34890990

RESUMO

Chronic kidney disease (CKD) is a common comorbidity among patients with type 2 diabetes. Exposure to perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) has been linked to poorer kidney function in general population, but the related studies in individuals with diabetes were very limited. We aimed to examine the longitudinal associations of PFOA and PFOS exposure and CKD incidence among diabetes patients. Baseline levels of PFOA and PFOS were measured in serum in 967 diabetes patients from the Dongfeng-Tongji cohort. Multivariable logistic regression models were used to characterize the relationship between serum PFOA and PFOS levels and incident CKD risk (defined as estimated glomerular filtration rate (eGFR) < 60 mL/min/1.73 m2). During 10-years follow-up, 267 incident CKD cases were identified. Only PFOS level was significantly associated with lower risk of CKD incidence (adjusted OR: 0.67; 95%CI: 0.51, 0.88). Such inverse association was only observed among participants with lower eGFR levels (< 70 mL/min/1.73 m2), although the interaction did not achieve statistical significance. Notably, an inverted U-shaped relationship between eGFR and serum PFOS level (Pfor nonlinearity < 0.001) was observed based on the 1825 subjects with available data at baseline. PFOS exposure was negatively associated with CKD incidence in patients with diabetes, especially in those with baseline eGFR levels < 70 mL/min/1.73 m2. This may be explained by the implication of baseline kidney function on the serum PFAS concentrations which in turn affect the relationship between PFOS exposure and the incident CKD risk among diabetes.


Assuntos
Ácidos Alcanossulfônicos , Diabetes Mellitus Tipo 2 , Poluentes Ambientais , Fluorcarbonetos , Insuficiência Renal Crônica , Caprilatos , Diabetes Mellitus Tipo 2/induzido quimicamente , Diabetes Mellitus Tipo 2/epidemiologia , Humanos , Insuficiência Renal Crônica/induzido quimicamente , Insuficiência Renal Crônica/epidemiologia
20.
Gerontologist ; 62(7): 1006-1017, 2022 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34379115

RESUMO

BACKGROUND AND OBJECTIVES: Television viewing is the most common leisure activity in late life and may ease loneliness but encourage sedentary behavior. These associations may be particularly evident among older adults who live alone and who may lack other forms of companionship throughout the day. RESEARCH DESIGN AND METHODS: Adults aged 65+ (N = 257) participated, of whom 34% lived alone. Participants completed an initial interview followed by a 5- to 6-day data collection involving multimethods: (a) Electronically Activated Recorders (30 s every 7 min) provided audio recordings of television viewing, (b) Actical accelerometers objectively measured physical activity, and (c) ecological momentary assessments every 3 hr assessed social interactions. RESULTS: On average, older adults spent approximately 37% of their waking time (6.4 hr a day) watching television. Multilevel models revealed that television viewing occurred when participants were alone or with a spouse and was associated with a greater proportion of time sedentary, lower activity, and higher ratings of loneliness compared to when not watching television. Older adults who lived alone reported greater loneliness during 3-hr intervals when viewing television, but older adults who lived with others spent a greater proportion of time sedentary when viewing television. DISCUSSION AND IMPLICATIONS: Findings are discussed with regard to different rationales and ways of watching television-as compensation for social isolation or as a passive leisure activity with a social partner. We discuss ideas for research on additional aspects of television viewing and screen time in late life.


Assuntos
Solidão , Comportamento Sedentário , Idoso , Exercício Físico , Humanos , Recreação , Televisão
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