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1.
Cancer Nurs ; 40(2): 88-93, 2017 Mar/Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26925991

RESUMO

BACKGROUND: Intravenous chemotherapy administration is a high-risk process; attention must be paid to preventing errors that might occur during the administration of chemotherapy. OBJECTIVE: The aim of this study is to investigate whether the healthcare failure mode and effect analysis (HFMEA) is a valid proactive method to apply to chemotherapy administration in the Chinese oncology inpatient setting. METHODS: A multidisciplinary team created a flow diagram of the chemotherapy administration process and potential failure modes were identified and evaluated using a hazard-scoring matrix. Using a decision tree, failure mode recommendations were made. Chemotherapy error rates before and after the HFMEA were compared. RESULTS: A total of 5 failure modes were identified with high hazard scores, and 15 recommendations were made. After the intervention, the chemotherapy error rate decreased significantly from 2.05% to 0.17%. CONCLUSIONS: The complexity of intravenous chemotherapy makes it vulnerable to error, and with serious consequences. Multiple errors can occur during ordering, preparing, compounding, dispensing, and administering the chemotherapy. The process of HFMEA helped reduce the chemotherapy error rate in Chinese hospitalized patients. IMPLICATIONS FOR PRACTICE: Clinicians in oncology can take effective measures to avoid chemotherapy errors using the HFMEA.


Assuntos
Antineoplásicos/administração & dosagem , Análise do Modo e do Efeito de Falhas na Assistência à Saúde , Erros de Medicação/prevenção & controle , Neoplasias/tratamento farmacológico , Administração Intravenosa , China , Hospitalização , Humanos
2.
Med Sci Monit ; 22: 5131-5140, 2016 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-28024138

RESUMO

BACKGROUND We investigated the relationship of the polymorphisms of SET and MYND domain-containing protein 3 (SMYD3) with risk and prognosis of ovarian cancer. MATERIAL AND METHODS The polymerase chain reaction (PCR) amplification method was applied to detect the polymorphisms of variable number of tandem repeats (VNTR) in the SMYD3 gene promoter region for 156 patients with ovarian cancer (case group) and 174 healthy people (control group). Quantitative reverse transcription polymerase chain reaction and Western blot were applied to detect SMYD3 mRNA and protein expressions. RESULTS The frequencies of VNTR genotype 3/3 and allele genotype 3 in the case group were significantly higher than those in the control group, while the frequency of genotype 2/2 in the control group was significantly higher than that in case group (all P<0.05). The proportion of poorly differentiated patients carrying VNTR genotype 3/3 was significantly higher than the proportion of poorly differentiated patients carrying VNTR genotype 2/2+2/3, while the proportion of patients carrying genotype 3/3 with International Federation of Gynecology and Obstetrics (FIGO) stage III-IV disease was significantly higher than the proportion of patients carrying genotype 2/2 +2/3 with FIGO stage III-IV disease (all P<0.05). SMYD3 mRNA and protein expressions were higher in the patients carrying genotype 3/3 than they were in the patients with the 2/2+2/3 genotype (all P<0.05). The 5-year survival rate for patients carrying VNTR genotype 3/3 was significantly lower than that of patients carrying genotype 2/2+2/3, and Cox regression analysis showed that VNTR genotype 3/3 was an independent risk factor for ovarian cancer prognosis (all P<0.05). CONCLUSIONS VNTR genotype 3/3 of the SMYD3 gene was associated with the risk of ovarian cancer. The polymorphism of VNTR genotype could be recognized as an indicator for the poor prognosis of patients with ovarian cancer.


Assuntos
Predisposição Genética para Doença , Histona-Lisina N-Metiltransferase/genética , Repetições Minissatélites/genética , Neoplasias Ovarianas/genética , Adulto , Idoso , Sequência de Bases , Feminino , Frequência do Gene/genética , Humanos , Estimativa de Kaplan-Meier , Modelos Logísticos , Pessoa de Meia-Idade , Análise Multivariada , Prognóstico , Modelos de Riscos Proporcionais , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Medição de Risco , Fatores de Risco , Adulto Jovem
3.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 36(1): 94-8, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-26955686

RESUMO

OBJECTIVE: To observe the effect of Baichanting Compound (BC) on dopamine (DA) in striatum of Parkinson's disease (PD) mice, and to screen the optimal component proportion. METHODS: The PD model was established in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced C57BL/6 mice. By using uniform design, they were intervened by three extracts of BC in different proportions [Acanthopanax senticosus extract (X1): white peony root extract (X2): Uncaria rhynchophylla extract (X3) = 30.00: 34.92: 82.50, 48.00: 19.98: 72.19, 18.00: 44.88: 61.88, 36.00: 29.94: 51.56, 54.00: 15.00: 41.25, 24.00: 39.90: 30.94, 42.00: 24.96: 20.63). Equal volume of 5% carboxymethylcellulose sodium was administered to mice in the model group and the normal group by gastrogavage. All medication was lasted for 20 successive days. The dopamine (DA) content was determined by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS). Except 10 in the normal group, 20 PD model mice were screened and divided into the model group and the BC group (with the optimal proportion) according to random digit table. BC extract in optimal proportion was administered to mice in the BC group by gastrogavage, while equal volume of 5% carboxymethylcellulose sodium was administered to mice in the model group and the normal group by gastrogavage. All medication was lasted for 20 successive days. Praxiology was observed in each group. DA content in striatum was also detected. Results Compared with the normal group, the DA content in striatum decreased significantly in the model group (P < 0.01), suggesting a successful PD modeling. Compared with the model group, the DA content in striatum increased significantly in 1 and 2 groups (P<0.05). According to results of quadratic polynomial stepwise regression statistics, the regression equation obtained was: Y = 0.265 + 0.026 X 2 - 0.056 X 3 + 0.334 x 10(-3) x X1 x X3 + 0.691 x 10(-3) X X3(2). X3 extract was the main factor influencing the effectiveness (P < 0.01). The optimal proportion of BC was predicted by the regression equation: X1 = 54.00 mg/(kg x d), X2 = 44.88 mg/(kg x d), the X3 = 82.50 mg/(kg x d). The pole climbing time was shortened, times of autonomic activities increased, DA content was elevated, all with statistical difference in BC groups (P < 0.01, P < 0.05). CONCLUSION: BC could increase DA content in PD model mice with the optimal proportion as 54.00: 44.88: 82.50.


Assuntos
Dopamina/metabolismo , Medicamentos de Ervas Chinesas/uso terapêutico , Doença de Parkinson/tratamento farmacológico , 1-Metil-4-Fenil-1,2,3,6-Tetra-Hidropiridina , Animais , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/farmacologia , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos C57BL , Atividade Motora , Doença de Parkinson/metabolismo
4.
Chin Med J (Engl) ; 128(18): 2498-504, 2015 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-26365969

RESUMO

BACKGROUND: High-dose chemotherapy followed by autologous stem cell transplantation (ASCT) is a promising approach for lymphomas. This study aimed to evaluate the effect of ifosfamide, cisplatin or carboplatin, and etoposide (ICE)-based regimen as a mobilization regimen on relapsed, refractory, or high-risk aggressive lymphoma. METHODS: From June 2001 to May 2013, patients with lymphomas who mobilized by ICE-based regimen for ASCT were analyzed in this retrospective study. The results of the autologous peripheral blood stem cells collection, toxicity, engraftment after ICE-based mobilization regimen were analyzed in this study. Furthermore, risk factors for overall survival (OS) and progression free survival (PFS) were evaluated by univariate analysis. RESULTS: The stem cells were mobilized using ICE-based regimen plus rituximab or ICE-based regimen alone in 12 patients and 54 patients, respectively. The results of stem cell mobilization were excellent. Ninety-seven percentages of the patients had the stem cell collection of at least 2.0 × 10 6 CD34 + cells/kg and 68% had at least 5 × 10 6 CD34 + cells/kg. Fifty-eight percentage of the patients experienced Grade 4 neutropenia, 20% developed febrile neutropenia, and only 12% had Grade 4 thrombocytopenia. At a median follow-up of 63.8 months, the 5-year PFS and OS were 64.4% and 75.3%, respectively. CONCLUSION: ICE is a powerful regimen for stem cell mobilization in patients with lymphomas.


Assuntos
Antineoplásicos/uso terapêutico , Carboplatina/uso terapêutico , Cisplatino/uso terapêutico , Etoposídeo/uso terapêutico , Mobilização de Células-Tronco Hematopoéticas/métodos , Ifosfamida/uso terapêutico , Linfoma/tratamento farmacológico , Transplante de Células-Tronco/métodos , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Transplante Autólogo , Adulto Jovem
5.
Yao Xue Xue Bao ; 50(5): 627-32, 2015 May.
Artigo em Chinês | MEDLINE | ID: mdl-26234148

RESUMO

Peptide cyclization, a pivotal approach to modifying linear precursors of proteins and pepticles, has been used to enhance their biological activities and serum stabilities. Recently, sortase A (SrtA) from Staphyloccus aureus becomes a promising new technology for efficiently incorporating site specific modifications into proteins, conjugating the cell surface and cyclizing the linear peptides. In this study, we constructed two recombinant expression systems, one with chitin binding domain and the other with six-histidine tag and chitin binding domain on the N-terminal of SrtA, separately. The results of enzymatic kinetics indicate that the two recombinant tags do not impair the transpeptidase activity of SrtA compared with the standard reaction reported under the same reaction condition. The two synthesized peptides with N-ternimal three glycines and C-terminal penta-amino acid motif, LPETG, were cyclized using immobilized and recycled SrtA. The SrtA-based cyclization promises to represent a simple method for easy and efficient enzymatic synthesis of large cyclic peptides.


Assuntos
Aminoaciltransferases/metabolismo , Proteínas de Bactérias/metabolismo , Cisteína Endopeptidases/metabolismo , Enzimas Imobilizadas/metabolismo , Peptídeos Cíclicos/biossíntese , Peptídeos/metabolismo , Ciclização , Cinética , Staphylococcus aureus/enzimologia
6.
Yao Xue Xue Bao ; 49(6): 905-12, 2014 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-25212039

RESUMO

Three cyclotides were isolated from the whole plant of Viola yedoensis in this study. The two, vary peptide E and cycloviolacin Y5, were previously reported, and a novel cycloviolacin VY1 was characterized according to the interpretation of MS/MS fragmentation of peptides which were produced from the reduced and alkylated parent peptide with the digestion of Endo Lys-C, trypsin and chymotrypsin, separately. The stability of remarkable resistance to proteolytic degradation by trypsin and chymotrypsin, and that of thermal denaturation was confirmed again. Besides, the IC50 value of cycloviolacin VY1 against influenza A H1N1 virus was (2.27 +/- 0.20) microg x mL(-1). It is the first cyclotide reported with anti-influenza A H1N1 virus activity in vitro assay.


Assuntos
Antivirais/farmacologia , Ciclotídeos/farmacologia , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Viola/química , Antivirais/isolamento & purificação , Espectrometria de Massas em Tandem
7.
Tumour Biol ; 35(10): 10419-27, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25053592

RESUMO

The objective of this study is to evaluate whether high-dose chemotherapy is more efficacious than standard-dose chemotherapy in the treatment of primary well-differentiated osteosarcoma. The Cochrane systematic evaluation method was adopted. A database search was conducted in MEDLINE, Embase, OVID, the Cochrane Central Register of Controlled Trials database and the Chinese Biomedical Literature CD-ROM Database. The quality of the included studies was jointly evaluated by two reviewers, and homogeneous studies were included for meta-analysis. A total of five studies were included in this meta-analysis, with 1,415 subjects with primary, nonmetastatic, well-differentiated osteosarcoma in the limbs. No statistically significant differences were found between the high-dose chemotherapy group and the low-dose group in 5-year event-free survival [RR 1.04, 95 %CI (0.95, 1.13)], 5-year overall survival [RR 1.02, 95 %CI (0.95, 1.10)], local recurrence rate [RR 0.90, 95 %CI (0.59, 1.39)], proportion of subjects with good histological response [RR 0.93, 95 %CI (0.81, 1.07)], or limb salvage rate [RR 0.97, 95 %CI (0.92, 1.02)]. A statistically significant difference was observed in the 5-year event-free survival between the subjects with good histological response to preoperative chemotherapy and the subjects with poor histological response [RR 1.55, 95 %CI (1.19, 2.00), P < 0.001]. High-dose chemotherapy did not show superior efficacy to low-dose chemotherapy in the treatment of primary well-differentiated osteosarcoma. Further high-quality randomized controlled trials are needed to provide additional reliable evidence for our observation.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Neoplasias Ósseas/tratamento farmacológico , Osteossarcoma/tratamento farmacológico , Neoplasias Ósseas/patologia , Diferenciação Celular , Humanos , Osteossarcoma/patologia , Ensaios Clínicos Controlados Aleatórios como Assunto
8.
Phytochemistry ; 104: 105-13, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24833035

RESUMO

Phytochemical investigation on Forsythia suspensa (Thunb.) Vahl afforded 10 compounds, including quinoid glycosides, lignan glycosides, phenylethanoid glycoside and allylbenzene glycoside together with 13 known ones. Their structures were established based on extensive spectroscopic data analyses, including IR, UV, HRESIMS, 1D NMR and 2D NMR. Absolute configurations were determined by ECD calculation method and chemical degradation. In addition, all compounds were evaluated for their antiviral activity against influenza A (H1N1) virus and several were further evaluated against respiratory syncytial virus (RSV) in vitro. Among them, two previously known compounds showed significant activities against RSV with EC50 values of 3.43 and 6.72 µM.


Assuntos
Antivirais/farmacologia , Forsythia/química , Glicosídeos/farmacologia , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Lignanas/farmacologia , Álcool Feniletílico/farmacologia , Animais , Antivirais/química , Antivirais/isolamento & purificação , Linhagem Celular , Cães , Frutas/química , Glicosídeos/química , Glicosídeos/isolamento & purificação , Humanos , Lignanas/química , Lignanas/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Álcool Feniletílico/química , Álcool Feniletílico/isolamento & purificação , Vírus Sinciciais Respiratórios/efeitos dos fármacos
9.
Asian Pac J Cancer Prev ; 15(2): 763-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24568492

RESUMO

AIM: To study the contribution of genetic variation in RAD51 to risk of esophageal squamous cell carcinoma (ESCC). METHODS: Three single nucleotide polymorphisms (SNPs) in RAD51 (rs1801320, rs4144242 and rs4417527) were genotyped in 316 ESCC patients and 316 healthy controls in Anyang area of China using PCR- RFLP (polymerase chain reaction-restriction fragment length polymorphism). Demographic variables between cases and controls were statistically compared by T test and Chi-square test. Hardy-Weinberg equilibrium was evaluated by the Chi-square test. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to measure any association with ESCC. Haplotype frequencies were estimated by Phase 2.1. RESULT: The genotype frequencies of rs1801320, rs4144242 and rs4417527 in patients with ESCC demonstrated no significant differences from those in control group (P>0.05). When the haplotypes of these three SNPs were constructed and their relationships with ESCC risk investigated, however, CGG was observed to increase the risk (P=0.020, OR=2. 289). CONCLUSIONS: There was no association between the three SNPs of RAD51 and ESCC susceptibility in our Chinese population. However, the CGG haplotype might be a risk factor.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Carcinoma de Células Escamosas/genética , Neoplasias Esofágicas/genética , Polimorfismo de Nucleotídeo Único/genética , Rad51 Recombinase/genética , Carcinoma de Células Escamosas/epidemiologia , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , China/epidemiologia , Neoplasias Esofágicas/epidemiologia , Neoplasias Esofágicas/patologia , Feminino , Seguimentos , Predisposição Genética para Doença , Genótipo , Haplótipos , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Prognóstico , Fatores de Risco
10.
Biomarkers ; 19(1): 81-5, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24410190

RESUMO

The purpose of the study was to explore the significance of FGFR4 protein expression in colorectal cancer. Immunohistochemistry showed 46.8% (148/316) tumors positive for FGFR4 and 7.3% (23/316) for adjacent normal specimens. FGFR4 positivity was correlated with shortened disease free survival (DFS) and overall survival (OS). Multivariate analysis revealed that FGFR4 was an independent prognostic factor. FGFR4 silencing markedly reduced the migration and invasion capacity of colorectal cancer cell lines. These results suggest FGFR4 is a potential prognostic and therapeutic marker for colorectal cancer.


Assuntos
Biomarcadores Tumorais/metabolismo , Neoplasias Colorretais/metabolismo , Receptor Tipo 4 de Fator de Crescimento de Fibroblastos/metabolismo , Idoso , Biomarcadores Tumorais/genética , Linhagem Celular Tumoral , Movimento Celular , Neoplasias Colorretais/mortalidade , Feminino , Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Prognóstico , Modelos de Riscos Proporcionais , Receptor Tipo 4 de Fator de Crescimento de Fibroblastos/genética
11.
Asia Pac J Oncol Nurs ; 1(1): 46-49, 2014 Apr-Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27981082

RESUMO

OBJECTIVE: Nurse-led telephone follow-up is effective in meeting information and psycho-social needs. We explored the potential effects of nurse-led telephone follow-up for patients treated with chemotherapy in China. METHODS: A quasi-experimental study was employed in the research. 300 cases of cancer inpatients in a cancer hospital in Beijing during July-October 2012 were selected by convenience sampling. To compare the satisfaction and response regarding to chemotherapy adverse side effects, patients who discharged on Monday and Friday were provided with telephone follow-up. Patients who discharged on Tuesday, Wednesday and Thursday received routine care. RESULTS: Via telephone follow-up, patient satisfaction relating to nursing care increased. Moreover, their response to chemotherapy adverse side effects showed a significant difference. CONCLUSION: Telephone follow-up by specialist nurses may be a feasible option. It was well received by patients, with no physical or psychological disadvantage.

12.
Yao Xue Xue Bao ; 46(7): 753-61, 2011 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-22010343

RESUMO

Human enterovirus 71 (EV71) is one of the major etiological agents for the hand, foot, and month disease (HFMD) and is causing frequent, widespread occurrence in the mainland of China. The single positive-stranded RNA genome of EV71 is translated into a single polyprotein which is autocleavaged into structural and nonstructural proteins. The functions of many nonstructural proteins characterized in the life cycle of virus are potential targets for blocking viral replication. This article reviews the studies of the structures and functions of nonstructural proteins of EV71 and the anti-enterovirus 71 drugs targeting on these nonstructural proteins.


Assuntos
Antivirais/farmacologia , Enterovirus Humano A/enzimologia , Doença de Mão, Pé e Boca/virologia , Terapia de Alvo Molecular , Peptídeo Hidrolases/química , Proteínas não Estruturais Virais/química , Enterovirus Humano A/genética , Enterovirus Humano A/isolamento & purificação , Doença de Mão, Pé e Boca/tratamento farmacológico , Humanos , Peptídeo Hidrolases/metabolismo , Peptídeo Hidrolases/fisiologia , Inibidores de Proteínas Quinases/farmacologia , RNA Viral/genética , Proteínas não Estruturais Virais/metabolismo , Proteínas não Estruturais Virais/fisiologia , Replicação Viral/efeitos dos fármacos
13.
Ying Yong Sheng Tai Xue Bao ; 21(5): 1264-9, 2010 May.
Artigo em Chinês | MEDLINE | ID: mdl-20707111

RESUMO

By using sustainable yield index (SYI), this paper analyzed the sustainability of maize, rice, and wheat yields at 20 long-term fertilization experiment sites in China under different fertilization system and ecological conditions. The SYI value of test crops varied significantly with fertilization system, crop kind, effective accumulated temperature, and sunshine hour. Irrespective of fertilization, the SYI value of rice was generally higher than that of maize and wheat. Under long-term no fertilization, the SYI values of rice, wheat, and maize were lower, being 0.55, 0.44, and 0.43, respectively. Fertilization, especially a combined application of NPK and manure, promoted the sustainability of crop yields, with the SYI values of rice, wheat, and maize being 0.66, 0.58, and 0.57, respectively. Under the application of N and NK, the SYI values ranged in 0. 36-0. 47. SYI value > 0.55 represented the better sustainability of crop yields, while SYI < 0.45 represented a worse one. Longitude, altitude, and weather factors also affected the SYI values, especially that of maize. Under no fertilization, maize had the largest variation of SYI value, followed by wheat, and rice. Our results indicated that a combined application of chemical fertilizers with manure benefited high and stable yielding, being the optimal fertilization mode for maintaining the sustainability of grain yield.


Assuntos
Conservação dos Recursos Naturais , Produtos Agrícolas/crescimento & desenvolvimento , Ecossistema , Fertilizantes , China , Fatores de Tempo
14.
J Pharm Biomed Anal ; 53(4): 1011-4, 2010 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-20674218

RESUMO

Proteomics technology was for the first time applied to investigate the changes of serum proteins levels in type 2 diabetes mellitus (T2DM) rat model after treated by Chinese traditional medicine Tianqi Jiangtang Capsule (ten normal Wistar rats, ten with T2DM and ten with T2DM administrated by Tianqi Jiangtang Capsule). In addition to two-dimensional polyacrylamide gel electrophoresis (2-DE), serum protein profiling in the three groups was further performed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-TOF/MS). 11 visualized spots were differentially regulated and identified as diabetes-associated proteins. All the samples in three groups were then analyzed by ELISA and estimated the 7 proteins which were found to vary. The distinct effect of T2DM induction on the pattern of rat serum includes the down-regulation of Apolipoprotein E, Apolipoprotein A-I, Ig gamma-2A chain C region, and up-regulation of Transthyretin (TTR), Haptoglobin (Hp), Serum amyloid P-componen (SAP), Prothrombin. The majority of those protein levels were interestingly restored to those of healthy rats after Tianqi Jiangtang Capsule treatment.


Assuntos
Proteínas Sanguíneas/análise , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Tipo 2/tratamento farmacológico , Medicina Tradicional Chinesa , Proteômica , Aloxano , Animais , Cápsulas , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Tipo 2/sangue , Ensaio de Imunoadsorção Enzimática , Haptoglobinas/análise , Pré-Albumina/análise , Ratos , Ratos Wistar , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
15.
Acta Crystallogr Sect E Struct Rep Online ; 67(Pt 1): o69, 2010 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-21522780

RESUMO

In the title compound, C(14)H(11)FN(2)O(2), an intra-molecular O-H⋯N hydrogen bond influences the mol-ecular conformation; the two benzene rings form a dihedral angle of 18.4 (3)°. The F atom is disordered over two positions in a 0.717 (5):0.283 (5) ratio. In the crystal, inter-molecular N-H⋯O hydrogen bonds link the mol-ecules into chains extending along the c axis.

16.
Acta Crystallogr Sect E Struct Rep Online ; 67(Pt 1): o70, 2010 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-21522782

RESUMO

The mol-ecule of the title compound, C(15)H(13)FN(2)O(2), exists in a trans configuration with respect to the methyl-idene unit. The two benzene rings form a dihedral angle of of 64.7 (2)°. In the crystal, mol-ecules are linked through N-H⋯O hydrogen bonds into chains propagating along the c axis.

17.
Yao Xue Xue Bao ; 45(11): 1402-9, 2010 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-21361040

RESUMO

The cyclotides are a family of cyclic "mini" proteins that occur in Violaceae, Rubiaceae and Cucurbitaceae plant families and contain a head-to-tail cyclic backbone and a cystine knot arranged by three disulfide bonds. To study the natural cyclotides of V tianshanica, dried herb was extracted with 50% ethanol, and the concentrated aqueous extract was subjected to a solvent-solvent partitioning between water and hexane, ethyl acetate and n-butanol, separately. The n-butanol extract containing cyclotides was subjected to column chromatography over Sephadex LH-20, eluted with 30% methanol. The subfractions were directly reduced by DTT and analyzed by reverse-phase HPLC. The peaks with different retention times were shown on the profile of RP-HPLC and collected. The cyclotides were speculated based on masses range from 3 000 to 3 500 Da. The purified cyclotides were reduced with DTT, alkylated with iodoacetamide, and then were cleaved with endoproteinase Glu-C, endoproteinase Lys-C and Trypsin, separately. The digested peptides were purified on RP-HPLC and analyzed on MALDI TOF/TOF analyzer. A new cyclotide, cycloviolacin T1 and a reported cyclotide varv E were systemically determined using MALDI TOF/TOF system. So the method for the isolation and characterization of cyclotides was quickly built up in succession.


Assuntos
Ciclotídeos/isolamento & purificação , Viola/química , Sequência de Aminoácidos , Cromatografia Líquida de Alta Pressão , Ciclotídeos/química , Dados de Sequência Molecular , Estrutura Molecular , Plantas Medicinais/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Espectrometria de Massas em Tandem
18.
Yi Chuan ; 32(11): 1187-94, 2010 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-21513171

RESUMO

NADPH-cytochrome P450 reductase (CPR), a partner for P450 monooxygenases, serves as the electron donor to almost all eukaryotic cytochrome P450s. One cDNA (TchCPR) encoding cytochrome P450 reductase of T. chinensis was isolated from callus cells. The cDNA contains an open reading frame of 2154 nucleotides which encodes a protein of 717 amino acid residues. The TchCPR has higher similarity to other CPRs of gumnosperms (>82%) than that of angiosperms (<74%). The recombinant full-length TchCPR and a series of N-terminal truncated constructs with N-terminal fusion of His Tag were obtained and induced to express in E. coli B121(DE3), and then purified using affinity chromatography. The truncated forms of N-terminal more than 61 amino acid residues could be efficiently expressed while the truncated mutant of N-terminal 48 amino acid residues and the wild-type TchCPR were not successfully expressed in E. coli cells. The activity of the truncated TchCPR was assayed by measuring the reduction of cytochrome C. The electron transfer activity of the recombinantly purified CPRT61 was 1.6057 nmol of cytochrome C reduced per min per microg TchCPR reductase, and it is higher than that of the other four truncated forms.


Assuntos
DNA Complementar/genética , NADPH-Ferri-Hemoproteína Redutase/genética , NADPH-Ferri-Hemoproteína Redutase/metabolismo , Taxus/enzimologia , Sequência de Aminoácidos , Clonagem Molecular , Transporte de Elétrons , Evolução Molecular , Expressão Gênica , Dados de Sequência Molecular , NADPH-Ferri-Hemoproteína Redutase/química , NADPH-Ferri-Hemoproteína Redutase/isolamento & purificação , Filogenia , Análise de Sequência de DNA , Taxus/genética
19.
Ying Yong Sheng Tai Xue Bao ; 20(9): 2142-8, 2009 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-20030135

RESUMO

Field experiments were conducted on three typical farmland soils (loess soil, fluvo-aquic soil, and cinnamon fluvo-aquic soil) in Northern China to study the grain yield, phosphorus agronomic efficiency (PAE), and phosphorus use efficiency (PUE) of wheat under effects of long-term fertilizations. Seven treatments were installed, i.e., non-fertilization (CK), nitrogen fertilization (N), nitrogen-potassium fertilization (NK), nitrogen-phosphorus fertilization (NP), nitrogen-phosphorus-potassium fertilization (NPK), NPK plus straw returning (NPKS), and NPK plus manure application (NPKM). The averaged wheat grain yields under long-term P fertilizations (treatments NP, NPK, NPKS, and NPKM) ranged from 2914 kg x hm(-2) to 6219 kg x hm(-2), being 200%-400% higher than those under no P fertilizations (treatments CK, N, and NK), and no significant differences were observed between the P fertilizations. In the early years of the experiment, the PAE in treatment NPK on the loess soil, fluvo-aquic soil, and cinnamon fluvo-aquic soil was 17.0 kg x kg(-1), 20.3 kg x kg(-1), and 13.3 kg x kg(-1), and the PUE was 15.3%, 31.2%, and 23.8%, respectively. After 15-year fertilization, the PAE and PUE in treatment NPK increased annually by 3.9 kg x kg(-1) and 1.3% on loess soil, 2.5 kg x kg(-1) and 0.9% on fluvo-aquic soil, and 2.8 kg x kg(-1) and 1.0% on cinnamon fluvo-aquic soil, respectively. There were no significant differences in the PAE and PUE among the P treatments for the same soils. In Northern China, long-term P fertilization could increase the wheat grain yield and PUE significantly, and the mean annual increase of PAE and PUE in treatment NPKM was higher on loess soil than on fluvo-aquic soil and cinnamon fluvo-aquic soil.


Assuntos
Fertilizantes , Fósforo/metabolismo , Solo/análise , Triticum/metabolismo , Biomassa , China , Fatores de Tempo , Triticum/crescimento & desenvolvimento
20.
Sheng Wu Gong Cheng Xue Bao ; 21(4): 547-52, 2005 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-16176090

RESUMO

Copper ion was necessary for the transcription of all laccase isozyme genes from Trametes sp. AH28-2, with higher concentrations of Cu2+ (1-2 mmol/L) being more favorable to the synthesis of laccase. In the glucose media containing 0.5 mmol/L Cu2+, the laccase activity of the supernate was rather low (44.3 u/L) and had an increase of 60.3% (71.0 u/L) when 4.0 mmol/L o-toluidine was added. Moreover, the activity reached up to 2584 u/L as the glucose was replaced by cellobiose. And Native-PAGE showed that LacA was the main laccase component if fungus was induced by o-toluidine or copper ions. It had been demonstrated by quantitative RT-PCR that the regulation of lacA expression, induced by o-toluidine, occurred at the transcriptional level, with the accumulation of mRNA transcripts being accompanied by the increase of laccase activity of the culture fluid. In addition, the structural gene of lacA interrupted by 10 introns was 2110 bp in length and the corresponding cDNA sequence was 1560 bp encoding a 520 aa protein, which had high similarities with other laccases from basidiomycetes. Furthermore, a length of 1881 bp of 5'-terminal sequence of transcription control of lacA, amplified by the improved inverse PCR, contained a TATA box, seven CAAT boxes as well as a number of putative cis-acting elements important for its expression, including five MREs, nine CreA-binding sites, four XREs, two STREs and seven nitrogen factor binding sites. The existence of these elements was well in agreement with the data obtained from Trametes sp. AH28-2 shaken-flask cultures.


Assuntos
Regiões 5' não Traduzidas/genética , Proteínas Fúngicas/metabolismo , Lacase/metabolismo , Trametes/genética , Sequência de Bases , Clonagem Molecular , Cobre/farmacologia , Proteínas Fúngicas/genética , Regulação Enzimológica da Expressão Gênica , Isoenzimas , Lacase/genética , Dados de Sequência Molecular , Trametes/enzimologia , Transcrição Genética
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