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1.
Medicine (Baltimore) ; 100(2): e24088, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33466170

RESUMO

RATIONALE: Takotsubo syndrome (TTS) is characterized by recovery of wall motion abnormalities and acute left ventricular dysfunction, which are often caused by acute physical or emotional stressors. It is rarely reported that TTS can be precipitated by change in position in the patient in the operating room. We report a case of a patient with a thoracic vertebral fracture who presented with TTS precipitated by changing from a supine to a prone position before percutaneous kyphoplasty (PKP) under local anesthesia. PATIENT CONCERNS: A 76-year-old man who was diagnosed with a fracture in a thoracic vertebra was sent to the operating room to undergo PKP under local anesthesia. Approximately 5 minutes after changing from a supine to a prone position, which is necessary for PKP, the patient experienced chest pain, headache, and sweating. DIAGNOSIS: A fracture in a thoracic vertebra; TTS. INTERVENTIONS: As a result of 12-lead electrocardiography, echocardiography, left ventriculogram, and cardiac catheterization, the diagnosis of TTS was retained, and supportive therapy was initiated. OUTCOMES: Two hours later, the patient's symptoms mitigated significantly and the ST segment returned to baseline. Four days later, echocardiography showed normal systolic function without wall motion abnormalities and the patient returned to the orthopedics ward for further treatment. LESSONS: It is necessary for anesthetists to recognize TTS which is life-threatening during monitored anesthetic care (MAC). We highlight the importance of being alerted to the possibility of TTS when managing patients with thoracic vertebral fractures undergoing surgery under local anesthesia.


Assuntos
Posicionamento do Paciente/efeitos adversos , Fraturas da Coluna Vertebral/complicações , Cardiomiopatia de Takotsubo/etiologia , Vértebras Torácicas/lesões , Idoso , Humanos , Cifoplastia , Masculino , Fraturas da Coluna Vertebral/fisiopatologia , Fraturas da Coluna Vertebral/cirurgia , Vértebras Torácicas/fisiopatologia
2.
Biomed Res Int ; 2020: 5348730, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33376726

RESUMO

Background: Dental caries is one of the most common chronic diseases observed in elderly patients. The development of preventive strategies for dental caries in elderly individuals is vital. Objective: The objective of the present study was to construct a generalized regression neural network (GRNN) prediction model for the risk assessment of dental caries among the geriatric residents of Liaoning, China. Methods: A stratified equal-capacity random sampling method was used to randomly select 1144 elderly (65-74 years) residents (gender ratio 1 : 1) of Liaoning, China. Data for the oral assessment, including caries characteristics, and questionnaire survey from each participant were collected. Multivariate logistic regression analysis was then performed to identify the independent predictors. GRNN was applied to establish a prediction model for dental caries. The accuracy of the unconditional logistic regression and the GRNN early warning model was compared. Results: A total of 1144 patients fulfilled the requirements and completed the questionnaires. The caries rate was 68.5%, and the main associated factors were toothache history, residence area, smoking, and drinking. We randomly divided the data for the 1144 participants into a training set (915 cases) and a test set (229 cases). The optimal smoothing factor was 0.7, and the area under the receiver operating characteristic curve for the GRNN model was 0.626 (95% confidence interval, 0.544 to 0.708), with a P value of 0.002. In terms of consistency, sensitivity, and specificity, the GRNN model was better than the traditional unconditional multivariate logistic regression model. Conclusion: Geriatric (65-74 years) residents of Liaoning, China, have a high rate of dental caries. Residents with a history of toothache and smoking habits are more susceptible to the disease. The GRNN early warning model is an accurate and meaningful tool for screening, early diagnosis, and treatment planning for geriatric individuals with a high risk of caries.

3.
Pharmacol Res ; : 105111, 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33065284

RESUMO

Macrophages, a type of myeloid immune cell, play essential roles in fighting against pathogenic invasion and activating T cell-mediated adaptive immune responses. As a major constituent of the tumor microenvironment (TME), macrophages play a complex role in tumorigenesis and tumor progression. They can inhibit tumor growth by releasing proinflammatory cytokines and exerting cytotoxic activities but principally contribute to tumor progression by promoting tumor proliferation, angiogenesis, and metastasis. The tumor-promoting hallmarks of macrophages have aroused widespread interest in targeting tumor-associated macrophages (TAMs) for cancer immunotherapy. Increasing preclinical and clinical studies suggest that TAMs are a promising target for cancer immunotherapy. To date, TAM-targeted therapeutic strategies have mainly been divided into two kinds: inhibiting pro-tumor TAMs and activating anti-tumor TAMs. We reviewed the heterogeneous and plastic characteristics of macrophages in the TME and the feasible strategies to target TAMs in cancer immunotherapy and summarized the complementary effect of TAM-targeted therapy with traditional treatments or other immunotherapies.

4.
Proc Natl Acad Sci U S A ; 117(19): 10414-10421, 2020 05 12.
Artigo em Inglês | MEDLINE | ID: mdl-32350143

RESUMO

The rise of oxygen on the early Earth about 2.4 billion years ago reorganized the redox cycle of harmful metal(loids), including that of arsenic, which doubtlessly imposed substantial barriers to the physiology and diversification of life. Evaluating the adaptive biological responses to these environmental challenges is inherently difficult because of the paucity of fossil records. Here we applied molecular clock analyses to 13 gene families participating in principal pathways of arsenic resistance and cycling, to explore the nature of early arsenic biogeocycles and decipher feedbacks associated with planetary oxygenation. Our results reveal the advent of nascent arsenic resistance systems under the anoxic environment predating the Great Oxidation Event (GOE), with the primary function of detoxifying reduced arsenic compounds that were abundant in Archean environments. To cope with the increased toxicity of oxidized arsenic species that occurred as oxygen built up in Earth's atmosphere, we found that parts of preexisting detoxification systems for trivalent arsenicals were merged with newly emerged pathways that originated via convergent evolution. Further expansion of arsenic resistance systems was made feasible by incorporation of oxygen-dependent enzymatic pathways into the detoxification network. These genetic innovations, together with adaptive responses to other redox-sensitive metals, provided organisms with novel mechanisms for adaption to changes in global biogeocycles that emerged as a consequence of the GOE.


Assuntos
Adaptação Biológica/genética , Arsênico/metabolismo , Oxigênio/metabolismo , Adaptação Biológica/fisiologia , Atmosfera , Evolução Biológica , Planeta Terra , Evolução Planetária , Fósseis , Oxirredução
5.
Ying Yong Sheng Tai Xue Bao ; 31(1): 333-339, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31957412

RESUMO

A large amount of azo dye wastewater is discharged into the environment, with serious risks to ecosystems and human health. Therefore, the development of treatment technology of azo dye wastewater was of practical significance. Photocatalytic methods showed promising application prospects due to easy to implement and effective. In this study, layered black phosphorus nanosheet (LBP) was used as a catalyst through liquid phase exfoliation method. Methyl orange (MO) was employed as a model azo dye to investigate the catalytic mechanism of LBP. The dominant transient species involved in the photocatalytic reaction was probed by quenching and fluorescence probe experiments. Degradation pathways of MO were proposed according to degradation products identified by the liquid chromatography-mass spectrometry. The results showed that degradation rate (kobs) of MO at acidic condition (pH=3.0) or alkaline condition (pH=11.0) was higher than that at neutral condition (pH=7.0). Degradation pathways of MO included that the azo bond was attacked by hydroxyl radicals (·OH) photogenerated by the LBP, and the intermediate products were further oxidized by ·OH to produce N, N-dimethyl-4-(2-p-phenylmethylhydrazine) aniline, 2-(dimethylamino)-5-((4(dimethylamino) phenyl) diazenyl) phenol and N, N-dimethyl-4-nitroaniline.


Assuntos
Ecossistema , Fósforo , Compostos Azo , Águas Residuárias
6.
World J Gastroenterol ; 26(47): 7444-7469, 2020 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-33384547

RESUMO

Although multiple drugs are accessible for recovering liver function in patients, none are considered efficient. Liver transplantation is the mainstay therapy for end-stage liver fibrosis. However, the worldwide shortage of healthy liver donors, organ rejection, complex surgery, and high costs are prompting researchers to develop novel approaches to deal with the overwhelming liver fibrosis cases. Mesenchymal stem cell (MSC) therapy is an emerging alternative method for treating patients with liver fibrosis. However, many aspects of this therapy remain unclear, such as the efficiency compared to conventional treatment, the ideal MSC sources, and the most effective way to use it. Because bone marrow (BM) is the largest source for MSCs, this paper used a systematic review approach to study the therapeutic efficiency of MSCs against liver fibrosis and related factors. We systematically searched multiple published articles to identify studies involving liver fibrosis and BM-MSC-based therapy. Analyzing the selected studies showed that compared with conventional treatment BM-MSC therapy may be more efficient for liver fibrosis in some cases. In contrast, the cotreatment presented a more efficient way. Nevertheless, BM-MSCs are lacking as a therapy for liver fibrosis; thus, this paper also reviews factors that affect BM-MSC efficiency, such as the implementation routes and strategies employed to enhance the potential in alleviating liver fibrosis. Ultimately, our review summarizes the recent advances in the BM-MSC therapy for liver fibrosis. It is grounded in recent developments underlying the efficiency of BM-MSCs as therapy, focusing on the preclinical in vivo experiments, and comparing to other treatments or sources and the strategies used to enhance its potential while mentioning the research gaps.

7.
Zootaxa ; 4603(1): zootaxa.4603.1.5, 2019 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-31717241

RESUMO

In this study, two new brackish-water species of Macrostomum (M. shenda n. sp. and M. spiriger n. sp.) collected from Shenzhen, China, were described based on morphological, histological, and molecular phylogenetic analyses. Macrostomum shenda n. sp. differs from its congeners in the oblique and non-thickening distal opening of its narrow funnel-shaped stylet. In addition, its sperm have neither bristles nor brush, and are surrounded by an undulating membrane in the mid-body region. In M. spiriger n. sp., the stylet is spirally twisted. Its reproductive apparatus has a seminal bursal pore opening exteriorly. Results of the 18S and 28S rDNA phylogenetic analyses also support the establishments of these two new species. Moreover, the 18S and 28S rDNA sequences of some species within Macrostomum in previous studies have been revised to avoid ambiguity, while Macrostomum dongyuanensis Wang Sun, 2015 was re-identified as a new record of M. quiritium Kolasa, 1973 from China.


Assuntos
Platelmintos , Animais , China , DNA Ribossômico , Filogenia , Reprodução
8.
Environ Microbiol ; 21(1): 182-196, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30325092

RESUMO

Little is known about microbial communities in the Ganges River, India and how they respond to intensive anthropogenic inputs. Here we applied shotgun metagenomics sequencing to study microbial community dynamics and function in planktonic samples collected along an approximately 700 km river transect, including urban cities and rural settings in upstream waters, before and after the monsoon rainy season. Our results showed that 11%-32% of the microbes represented terrestrial, sewage and human inputs (allochthonous). Sewage inputs significantly contributed to the higher abundance, by 13-fold of human gut microbiome (HG) associated sequences and 2-fold of antibiotic resistance genes (ARGs) in the Ganges relative to other riverine ecosystems in Europe, North and South America. Metagenome-assembled genome sequences (MAGs) representing allochthonous populations were detectable and tractable across the river after 1-2 days of (downstream) transport (> 200 km apart). Only approximately 8% of these MAGs were abundant in U.S. freshwater ecosystems, revealing distinct biodiversity for the Ganges. Microbial communities in the rainy season exhibited increased alpha-diversity and spatial heterogeneity and showed significantly weaker distance-decay patterns compared with the dry season. These results advance our understanding of the Ganges microbial communities and how they respond to anthropogenic pollution.


Assuntos
Biodiversidade , Monitoramento Ambiental , Microbiota/fisiologia , Rios/microbiologia , Estações do Ano , Esgotos , Cidades , Resistência Microbiana a Medicamentos/genética , Humanos , Índia , Metagenoma , Metagenômica , Microbiota/genética , Plâncton/microbiologia
9.
Medicine (Baltimore) ; 97(51): e12345, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30572422

RESUMO

We developed a bathroom safety management information system to decrease adverse nursing events, and observed the application of the self-developed safety management information system in neonatal bathroom.A total of 3482 newborns receiving neonatal bath and rooming in between May 2015 and May 2017, were enrolled in this study. Of the 3482 newborns, 1727 that did not use the safety management information system from May 1, 2015 to May 31, 2016, were considered as control group; and other 1755 that used the safety management information system from June 1, 2016 to May 31, 2017 were entered in observation group. The accident rate of adverse nursing events, the duration to check wristbands, response time of urgency call, quantitative data recording for nursing procedures, and pregnant women's and their families' satisfaction degree were compared between the 2 groups.The management information system possesses 4 functions including personal identification, nursing operation quantification, monitoring alarm and music function, and guidance on specialized knowledge and skills. The accident rate of adverse nursing events was significantly lower in the observation than in the control group (P < .05). The duration to check wristbands and the response time of urgency call were all significantly shorter in the observation group than in the control group (all P < .05). Quantitative data recording was significantly better in the observation than in the control group (P < .05). Satisfaction degree was significantly higher in the observation group (96.47%) than in the control group (89.69%) (P < .05). The wireless transmission information was exact and safe, and the system was sensitive and reliable.The system not only is clinically practical but also can enhance the safety of newborns and improve pregnant women's and their families' satisfaction degree.


Assuntos
Banhos , Recém-Nascido , Sistemas de Informação Administrativa , Segurança do Paciente , Toaletes , Banhos/métodos , Família , Feminino , Humanos , Monitorização Fisiológica/métodos , Enfermagem Neonatal/educação , Enfermagem Neonatal/métodos , Satisfação do Paciente , Gravidez
10.
Sci Rep ; 7(1): 7741, 2017 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-28798375

RESUMO

The toxic metalloid arsenic has been environmentally ubiquitous since life first arose nearly four billion years ago and presents a challenge for the survival of all living organisms. Its bioavailability has varied dramatically over the history of life on Earth. As life spread, biogeochemical and climate changes cyclically increased and decreased bioavailable arsenic. To elucidate the history of arsenic adaptation across the tree of life, we reconstructed the phylogeny of the arsM gene that encodes the As(III) S-adenosylmethionine (SAM) methyltransferase. Our results suggest that life successfully moved into arsenic-rich environments in the late Archean Eon and Proterozoic Eon, respectively, by the spread of arsM genes. The arsM genes of bacterial origin have been transferred to other kingdoms of life on at least six occasions, and the resulting domesticated arsM genes promoted adaptation to environmental arsenic. These results allow us to peer into the history of arsenic adaptation of life on our planet and imply that dissemination of genes encoding diverse adaptive functions to toxic chemicals permit adaptation to changes in concentrations of environmental toxins over evolutionary history.


Assuntos
Adaptação Biológica/efeitos dos fármacos , Adaptação Biológica/genética , Arsênico/toxicidade , Transferência Genética Horizontal , Metiltransferases/genética , Animais , Archaea/efeitos dos fármacos , Archaea/genética , Arsênico/metabolismo , Bactérias/efeitos dos fármacos , Bactérias/genética , Eucariotos/efeitos dos fármacos , Eucariotos/genética , Fungos/efeitos dos fármacos , Fungos/genética , Metilação , Metiltransferases/metabolismo , Modelos Biológicos , Filogenia
12.
Environ Microbiol ; 19(6): 2468-2482, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28447395

RESUMO

As an analogue of phosphorus, arsenic (As) has a biogeochemical cycle coupled closely with other key elements on the Earth, such as iron, sulfate and phosphate. It has been documented that microbial genes associated with As biotransformation are widely present in As-rich environments. Nonetheless, their presence in natural environment with low As levels remains unclear. To address this issue, we investigated the abundance levels and diversities of aioA, arrA, arsC and arsM genes in estuarine sediments at low As levels across Southeastern China to uncover biogeographic patterns at a large spatial scale. Unexpectedly, genes involved in As biotransformation were characterized by high abundance and diversity. The functional microbial communities showed a significant decrease in similarity along the geographic distance, with higher turnover rates than taxonomic microbial communities based on the similarities of 16S rRNA genes. Further investigation with niche-based models showed that deterministic processes played primary roles in shaping both functional and taxonomic microbial communities. Temperature, pH, total nitrogen concentration, carbon/nitrogen ratio and ferric iron concentration rather than As content in these sediments were significantly linked to functional microbial communities, while sediment temperature and pH were linked to taxonomic microbial communities. We proposed several possible mechanisms to explain these results.


Assuntos
Arsênico/metabolismo , Bactérias/classificação , Bactérias/metabolismo , Biotransformação/fisiologia , Sedimentos Geológicos/microbiologia , Bactérias/genética , Biotransformação/genética , China , Meio Ambiente , RNA Ribossômico 16S/genética , Áreas Alagadas
13.
ACS Chem Biol ; 12(6): 1703-1710, 2017 06 16.
Artigo em Inglês | MEDLINE | ID: mdl-28448716

RESUMO

K-Ras4B is one of the most frequently mutated Ras isoforms in cancer. The signaling activity of K-Ras4B depends on its localization to the plasma membrane (PM), which is mainly mediated by its polybasic farnesylated C-terminus. On top of the constitutive cycles that maintain the PM enrichment of K-Ras4B, conditional phosphorylation at Ser181 located within this motif has been found to be involved in regulating K-Ras4B's cell distribution and signaling activity. However, discordant observations have undermined our understanding of the role this phosphorylation plays. Here, we report an efficient strategy for producing K-Ras4B simultaneously bearing phosphate, farnesyl, and methyl modifications on a preparative scale, a very useful in vitro system when used in concert with model biomembranes. By using this system, we determined that phosphorylation at Ser181 does not fully inhibit membrane binding and clustering of K-Ras4B but reduces its membrane binding affinity, depending on membrane fluidity. In addition, phosphorylated K-Ras4B maintains tight association with its cytosolic shuttle protein PDEδ. After delivering K-Ras4B containing nonhydrolyzable phosphoserine mimetic into cells, the protein displayed a decreasing PM distribution compared with nonphosphorylable K-Ras4B, implying that phosphorylation might facilitate the dissociation of K-Ras4B from the PM. In addition, phosphorylation does not alter the localization of K-Ras4B in the liquid-disordered lipid subdomains of the membrane but slightly alters the thermotropic properties of K-Ras4B-incorporated membranes probably due to minor differences in membrane partitioning and dynamics. These results provide novel mechanistic insights into the role that phosphorylation at Ser181 plays in regulating K-Ras4B's distribution and activity.


Assuntos
Membrana Celular/metabolismo , Proteínas Proto-Oncogênicas p21(ras)/metabolismo , Humanos , Modelos Biológicos , Fosforilação/fisiologia , Agregados Proteicos , Serina/metabolismo
14.
J Pept Sci ; 23(6): 438-444, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28429419

RESUMO

Prions are suspected as pathogen of the fatal transmissible spongiform encephalopathies. Strategies to access homogenous prion protein (PrP) are required to fully comprehend the molecular mechanism of prion diseases. However, the polypeptide fragments from PrP show a high tendency to form aggregates, which is a gigantic obstacle of protein synthesis and purification. In this study, murine prion sequence 90 to 230 that is the core three-dimensional structure domain was constructed from three segments murine PrP (mPrP)(90-177), mPrP(178-212), and mPrP(213-230) by combining protein expression, chemical synthesis and chemical ligation. The protein sequence 90 to 177 was obtained from expression and finally converted into the polypeptide hydrazide by chemical activation of a cysteine in the tail. The other two polypeptide fragments of the C-terminal were obtained by chemical synthesis, which utilized the strategies of isopeptide and pseudoproline building blocks to complete the synthesis of such difficult sequences. The three segments were finally assembled by sequentially using native chemical ligation. This strategy will allow more straightforward access to homogeneously modified PrP variants. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.


Assuntos
Ésteres/síntese química , Peptídeos/síntese química , Proteínas Priônicas/síntese química , Compostos de Sulfidrila/síntese química , Animais , Ésteres/química , Camundongos , Conformação Molecular , Peptídeos/química , Proteínas Priônicas/química , Compostos de Sulfidrila/química
15.
Nat Microbiol ; 2: 16270, 2017 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-28134918

RESUMO

Antibiotic resistance genes (ARGs) have moved from the environmental resistome into human commensals and pathogens, driven by human selection with antimicrobial agents. These genes have increased in abundance in humans and domestic animals, to become common components of waste streams. Estuarine habitats lie between terrestrial/freshwater and marine ecosystems, acting as natural filtering points for pollutants. Here, we have profiled ARGs in sediments from 18 estuaries over 4,000 km of coastal China using high-throughput quantitative polymerase chain reaction, and investigated their relationship with bacterial communities, antibiotic residues and socio-economic factors. ARGs in estuarine sediments were diverse and abundant, with over 200 different resistance genes being detected, 18 of which were found in all 90 sediment samples. The strong correlations of identified resistance genes with known mobile elements, network analyses and partial redundancy analysis all led to the conclusion that human activity is responsible for the abundance and dissemination of these ARGs. Such widespread pollution with xenogenetic elements has environmental, agricultural and medical consequences.


Assuntos
Bactérias/genética , DNA Bacteriano/análise , Farmacorresistência Bacteriana , Estuários , Genes Bacterianos , Sedimentos Geológicos/microbiologia , Bactérias/isolamento & purificação , China , Humanos , Sequências Repetitivas Dispersas , Reação em Cadeia da Polimerase em Tempo Real
17.
Ying Yong Sheng Tai Xue Bao ; 28(6): 1955-1961, 2017 Jun 18.
Artigo em Chinês | MEDLINE | ID: mdl-29745159

RESUMO

This experiment was conducted to study the responses of photosynthetic gas exchange parameters and the chlorophyll fluorescence parameters in leaves to soil drought. Furthermore, the drought resistance abilities of the endangered native Physocarpus amurensis and the introduced P. opulifolius as well as their differences were studied. The results showed that the leaves of P. opulifolius wilted significantly, while the leaf water content and water use efficiency of the native P. amurensis were higher on the 7th day after soil drought. Soil drought reduced the net photosynthetic rate, stomatal conductance, and transpiration rate in the leaves of the two Physocarpus species, while the observed decrease of P. opulifolius was significantly higher than that of P. amurensis. On the 7th day after soil drought, the intercellular CO2 concentration (Ci) of P. opulifolius was higher than that without drought treatment, while the Ci of P. amurensis was lower than that without drought treatment. The electron transfer rate (ETR) and photochemical quenching coefficient (qP) in leaves of P. amurensis were clearly decreased, while differences of the light energy capture efficiency (Fv'/Fm') in the PS2 reaction center were non-significant. However, Fv'/Fm', ETR, and qP in the lea-ves of P. opulifolius were all significantly decreased to greater extents compared to those in P. amurensis. On the 7th day after soil drought, a non-significant change was observed on the relative variable fluorescence (VJ) at site J of the OJIP curve of P. amurensis leaves, while VJ in leaves of P. opuli-folius was increased. The carbon assimilation ability of P. opulifolius leaves and the sensibility of PS2 function to soil drought were significantly higher than those of P. amurensis. The reduction in the photosynthetic capacity induced by soil drought was mainly due to the limitation of the stomatal factors for P. amurensis, but mainly due to the limitation of the non-stomatal factors for P. opulifolius.


Assuntos
Clorofila , Secas , Fotossíntese , Dióxido de Carbono , Folhas de Planta , Água
18.
PLoS One ; 11(11): e0167269, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27898715

RESUMO

Hand, foot, and mouth disease (HFMD) is an arising public health problem in Asia, including China. Epidemiological data is necessary to enable judicious public health responses and interventions. We analyzed the epidemiological and laboratory data of 759,301 HFMD cases reported to the Hunan Provincial Center for Disease Control and Prevention from 1 January 2009 to 31 December 2014. Univariate and multivariable conditional logistic regression analyses were used to identify risk factors of fatality in HFMD. The incidence of HFMD was highest among children aged 1-3 years, compared with other age groups. Of the total HFMD cases, 7,222 (0.95%) were considered severe and 338 (0.04%) were fatal. Enterovirus-A71 was the major cause of severe and fatal cases (65.75% and 88.78%, respectively). For severe cases, the median time from symptom onset to diagnosis was 0.5 days (interquartile range [IQR] 0-1.5 days); the median time from diagnosis to severe illness was 2 days (IQR 1-3 days). For fatal cases, the median time from symptom onset to diagnosis was 0.5 days (IQR 0-1.5 days); the median time from diagnosis to death was 1.5 days (IQR 0.5-2.5 days). In multivariable analysis, the abuse of antibiotic, glucocorticoid and pyrazolone in village clinics at basic medical institutions were identified as independent risk factors for HFMD fatal cases. In conclusion, our results suggest that the future direction to control and respond to HFMD is intensive surveillance of enterovirus-A71 and improving the ability to diagnose disease and treat patients, especially in basic medical institutions.


Assuntos
Enterovirus Humano A/fisiologia , Doença de Mão, Pé e Boca/epidemiologia , Antibacterianos/uso terapêutico , Estudos de Casos e Controles , Criança , Pré-Escolar , China/epidemiologia , Enterovirus Humano A/isolamento & purificação , Feminino , Glucocorticoides/uso terapêutico , Doença de Mão, Pé e Boca/diagnóstico , Doença de Mão, Pé e Boca/tratamento farmacológico , Doença de Mão, Pé e Boca/virologia , Humanos , Incidência , Lactente , Modelos Logísticos , Masculino , Análise Multivariada , Pirazolonas/uso terapêutico , Características de Residência , Fatores de Risco , Sorogrupo , Índice de Gravidade de Doença , Fatores de Tempo
19.
Mol Biosyst ; 12(6): 1764-7, 2016 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-27102587

RESUMO

A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.


Assuntos
Técnicas Biossensoriais , Cisteína Endopeptidases/química , Coloração e Rotulagem , Ubiquitina/química , Cisteína Endopeptidases/metabolismo , Transferência Ressonante de Energia de Fluorescência , Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina/química , Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina/metabolismo , Ubiquitina/metabolismo , Ubiquitina Tiolesterase , Ubiquitinação
20.
Environ Pollut ; 211: 1-8, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26736050

RESUMO

Microbe-mediated arsenic (As) metabolism plays a critical role in global As cycle, and As metabolism involves different types of genes encoding proteins facilitating its biotransformation and transportation processes. Here, we used metagenomic analysis based on high-throughput sequencing and constructed As metabolism protein databases to analyze As metabolism genes in five paddy soils with low-As contents. The results showed that highly diverse As metabolism genes were present in these paddy soils, with varied abundances and distribution for different types and subtypes of these genes. Arsenate reduction genes (ars) dominated in all soil samples, and significant correlation existed between the abundance of arr (arsenate respiration), aio (arsenite oxidation), and arsM (arsenite methylation) genes, indicating the co-existence and close-relation of different As resistance systems of microbes in wetland environments similar to these paddy soils after long-term evolution. Among all soil parameters, pH was an important factor controlling the distribution of As metabolism gene in five paddy soils (p = 0.018). To the best of our knowledge, this is the first study using high-throughput sequencing and metagenomics approach in characterizing As metabolism genes in the five paddy soil, showing their great potential in As biotransformation, and therefore in mitigating arsenic risk to humans.


Assuntos
Arsênico/metabolismo , Bactérias/genética , Genes Microbianos , Microbiologia do Solo , Poluentes do Solo/metabolismo , Arseniatos , Arsênico/análise , Arsenitos , Bactérias/metabolismo , Biotransformação , Genes Bacterianos , Humanos , Metilação , Oryza , Solo/química , Poluentes do Solo/análise
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